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https://www.readbyqxmd.com/read/28087978/identification-of-gonadotropin-releasing-hormone-metabolites-in-greyhound-urine
#1
David Palmer, Katie Rademaker, Ingrid Martin, Joan Hessell, Rob Howitt
Gonadotropin-releasing hormone (GnRH) is a 10-residue peptide hormone that induces secretion of luteinizing hormone (LH) and follicle-stimulating hormone into the blood from the pituitary gland. In males, LH acts on the testes to produce testosterone. The performance enhancing potential of testosterone makes administration of exogenous GnRH a concern in sports doping control. Detection of GnRH abuse is challenging owing to its rapid clearance from the body and degradation in urine. Following recent investigations of GnRH abuse in racing greyhounds in New Zealand, we carried out a GnRH administration study in greyhounds in an attempt to identify GnRH metabolites that might provide more facile detection of GnRH abuse; little information is available on in vivo metabolites of exogenous GnRH in any species and none in dogs...
January 14, 2017: Drug Testing and Analysis
https://www.readbyqxmd.com/read/28077887/examination-of-the-predictive-power-of-electromyography-and-urodynamic-study-in-patients-with-cauda-equina-syndrome-horse-tail-syndrome
#2
Mohammadreza Shahmohammadi, Reza Jalil Khoshuod, Alireza Zali, Amir Saied Seddeghi, Nima Mohseni Kabir
BACKGROUND: Cauda equina syndrome is a rare disorder that causes loss of Lumbar plexus function (nerve roots) lower than conus medullaris. No risk factor has been defined for this disease yet. Due to the high morbidity of Cauda equina syndrome and lack of sufficient information about the connection between the disease and urodynamic findings and EMG (Electromyography) findings, the need for this comprehensive study is felt. OBJECTIVE: The aim is to determine the predictive power of findings resulted from urodynamics and electromyography of perineal region and around sphincter in the clinical cure rate of urination in patients with urinary retention followed by Cauda equina syndrome...
October 2016: Acta Informatica Medica: AIM
https://www.readbyqxmd.com/read/28077541/the-role-of-bicarbonate-precursors-in-balanced-fluids-during-haemorrhagic-shock-with-and-without-compromised-liver-function
#3
B Ergin, A Kapucu, P Guerci, C Ince
BACKGROUND: Lactate, acetate, and gluconate are anions used in balanced resuscitation fluids, of which lactate and acetate are considered bicarbonate precursors. This study investigated the role of the liver in the ability of balanced and unbalanced solutions to correct acid-base alterations and renal haemodynamics and microvascular oxygenation in a rat model of resuscitated haemorrhagic shock. METHODS: Ringer's lactate, Ringer's acetate, PlasmaLyte, or normal saline were administered following haemorrhagic shock in the presence or absence of a 70% partial liver resection...
October 2016: British Journal of Anaesthesia
https://www.readbyqxmd.com/read/28074773/low-dose-dialysis-combined-with-low-protein-intake-can-maintain-nitrogen-balance-in-peritoneal-dialysis-patients-in-poor-economies%C3%A2
#4
Chun-Yan Su, Tao Wang, Xin-Hong Lu, Sha Ma, Wen Tang, Pei-Yu Wang
OBJECTIVE: Due to limited economic conditions, we tried to provide "fitted" dialysis doses instead of the doses recommended by the international guidelines to the individual patients. In the present cross-sectional study, we studied the dialysis adequacy and nutritional status of 5 peritoneal dialysis patients who had a low dialysis dose (2 bags, 4,000 mL/day). METHODS: The 3-day dietary records were reviewed to calculate patients' energy, protein, and nitrogen intake (NI)...
January 11, 2017: Clinical Nephrology
https://www.readbyqxmd.com/read/28069228/cu-sns-sno2-nanoparticles-as-novel-sorbent-for-dispersive-micro-solid-phase-extraction-of-atorvastatin-in-human-plasma-and-urine-samples-by-high-performance-liquid-chromatography-with-uv-detection-application-of-central-composite-design-ccd
#5
Mehdi Dastkhoon, Mehrorang Ghaedi, Arash Asfaram, Maryam Arabi, Abbas Ostovan, Alireza Goudarzi
Separation and detection of residual drug in biological fluids has directly related to human health in term of their beneficial or side effects. In biological fluid samples (complex matrix which containing drug at very low level) conduction of preliminary efficient technique as good choice for pre-concentration and clean-up of real sample before their instrumental analysis is highly recommended. In this result technique, based on nano-structure material which poses higher available surface area and larger member of reactive sites led to significant improvement in characteristic performance of analytical method...
May 2017: Ultrasonics Sonochemistry
https://www.readbyqxmd.com/read/28063841/external-physical-vibration-lithecbole-promote-the-stone-clearance-of-upper-urinary-stone-after-retrograde-intrarenal-surgery-a-prospective-multi-center-randomized-controlled-trial
#6
Wenqi Wu, Zhou Yang, Changbao Xu, Xiaojian Gu, Sixing Yang, Songbai Liao, Rongjiang Wang, Wenxi Gao, Zhangqun Ye, Guohua Zeng
OBJECTIVE: To assess the efficacy and safety of External Physical Vibration Lithecbole (EPVL) for the treatment of residual stone after retrograde intrarenal surgery (RIRS). MATERIALS AND METHODS: A total of 173 patients (128 male and 45 female) were selected for the study. All the patients had residual fragments after RIRS for the renal or upper ureteral stones. They were prospectively randomized into two groups, one received EPVL one week after RIRS (group ER), another only underwent RIRS as a control (group CR)...
January 4, 2017: Journal of Urology
https://www.readbyqxmd.com/read/28062085/thulium-laser-enucleation-thulep-versus-transurethral-resection-of-the-prostate-in-saline-turis-a-randomized-prospective-trial-to-compare-intra-and-early-postoperative-outcomes
#7
G Bozzini, M Seveso, S Melegari, O de Francesco, N M Buffi, G Guazzoni, M Provenzano, A Mandressi, G Taverna
OBJECTIVE: To compare clinical intra and early postoperative outcomes between thulium laser transurethral enucleation of the prostate (ThuLEP) and transurethral bipolar resection of the prostate (TURis) for treating benign prostatic hyperplasia (BPH) in a prospective randomized trial. METHODS: The study randomized 208 consecutive patients with BPH to ThuLEP (n=102) or TURis (n=106). For all patients were evaluated preoperatively with regards to blood loss, catheterization time, irrigation volume, hospital stay and operative time...
January 3, 2017: Actas Urologicas Españolas
https://www.readbyqxmd.com/read/28060892/the-utility-of-post-void-residual-volume-versus-sphincter-electromyography-to-distinguish-between-multiple-system-atrophy-and-parkinson-s-disease
#8
Tatsuya Yamamoto, Masato Asahina, Yoshitaka Yamanaka, Tomoyuki Uchiyama, Shigeki Hirano, Miki Fuse, Yasuko Koga, Ryuji Sakakibara, Satoshi Kuwabara
OBJECTIVE: To determine the ability of sphincter electromyography (EMG) and post-void residual urine volume (PVR) during a free-flow study and a pressure-flow study (PFS) for distinguishing multiple system atrophy (MSA) from Parkinson's disease (PD). METHODS: We retrospectively reviewed 241 case records; both urodynamic study and sphincter EMG were performed in patients with MSA (n = 147) and PD (n = 94). RESULTS: There was a statistically significant difference (p < 0...
2017: PloS One
https://www.readbyqxmd.com/read/28046142/nitrogen-utilization-efficiency-and-prediction-of-nitrogen-excretion-in-sheep-offered-fresh-perennial-ryegrass
#9
Y G Zhao, A W Gordon, N E O'Connell, T Yan
Nitrogen excretion from sheep production systems is an important source of nitrate, ammonia, and nitrous oxide responsible for groundwater pollution and global warming. The present study aimed to identify key parameters influencing N utilization efficiency and develop prediction equations for manure N, feces N, and urine N outputs in sheep. Data used were collected from 82 sheep offered fresh perennial ryegrass () as the sole diet in 6 metabolism experiments (data from non-grass-only diets were not used). Sheep were from breeds of Highlander, Texel, Scottish Blackface, and Swaledale at the age of 5 to 18 mo and weighing from 24...
December 2016: Journal of Animal Science
https://www.readbyqxmd.com/read/28046138/detection-of-residues-in-urine-and-tissues-of-sheep-treated-with-trace-levels-of-dietary-ractopamine-hcl
#10
D J Smith, W L Shelver, A Marx
Qualitative assays are sometimes used as the sole basis for detecting drug residues in live animals or in animal products. Such assays have become increasingly sensitive as detection technologies have improved, yet the limitations of such assays to discriminate purposeful and accidental drug exposures remain poorly defined. A study was conducted to determine the ability of a ractopamine lateral flow assay to accurately detect incurred ractopamine residues in contaminated feeds and in sheep fed trace quantities of ractopamine HCl...
December 2016: Journal of Animal Science
https://www.readbyqxmd.com/read/28043112/the-influence-of-androgen-deprivation-therapy-on-prostate-size-and-voiding-symptoms-in-prostate-cancer-patients-in-korea
#11
Hoon Choi, Hong Chung, Jae Young Park, Jeong Gu Lee, Jae Hyun Bae
PURPOSE: The goal of this study is to investigate the effects of androgen deprivation therapy (ADT) on total prostate volume and lower urinary tract symptoms (LUTS). METHODS: Between January 2007 and June 2014, 110 patients who received androgen deprivation treatment were enrolled in this retrospective study. Clinical parameters and urodynamic parameters along with changes at follow-up were analyzed. Factors such as reduction in prostate volume, changes in LUTS, and prostate volume tertiles were compared 1 year after ADT...
December 2016: International Neurourology Journal
https://www.readbyqxmd.com/read/28043107/pregnancy-with-a-successful-vaginal-delivery-following-augmentation-enterocystoplasty-for-ketamine-cystitis-a-case-report
#12
Chiung-Hui Peng, Shang-Jen Chang, Stephen S Yang
A 28-year-old female with a 1-year history of ketamine abuse developed ketamine-associated urinary symptoms that were refractory to conservative treatment after the complete cessation of ketamine use. Smooth voiding with increased bladder capacity and minimal postvoid residual urine volume were achieved by performing an augmentation enterocystoplasty. An uneventful pregnancy with the vaginal delivery of a healthy baby occurred postoperatively.
December 2016: International Neurourology Journal
https://www.readbyqxmd.com/read/28040503/are-interferential-electrical-stimulation-and-diaphragmatic-breathing-exercises-beneficial-in-children-with-bladder-and-bowel-dysfunction
#13
Vesna D Zivkovic, Ivona Stankovic, Lidija Dimitrijevic, Mirjana Kocic, Hristina Colovic, Marina Vlajkovic, Andjelka Slavkovic, Milica Lazovic
OBJECTIVE: To evaluate the effects of interferential current (IC) stimulation and diaphragmatic breathing exercises (DBE) in children with bladder and bowel dysfunction. PATIENTS AND METHODS: Seventy-nine children with dysfunctional voiding and chronic constipation who were failures of primary care interventions were included in the prospective clinical study. All the children were checked for their medical history regarding LUTS and bowel habits. Physical examination including abdominal and anorectal digital examination were performed...
December 28, 2016: Urology
https://www.readbyqxmd.com/read/28029295/effectiveness-of-manual-bladder-expression-in-paraplegic-dogs
#14
Darren R Carwardine, Jeremy H Rose, Thomas R Harcourt-Brown, Nicolas Granger
OBJECTIVE To determine the effectiveness of manual bladder expression in paraplegic dogs by comparing urine volumes measured by use of intermittent catheterization and ultrasonography. ANIMALS 36 paraplegic dogs. PROCEDURES 93 measurements of bladder volume were collected for the 36 dogs. Residual urine volume was determined by use of intermittent urethral catheterization and estimated by use of ultrasonography. RESULTS Manual bladder expression voided a mean of 49% of urine from the bladder in this population of dogs...
January 2017: American Journal of Veterinary Research
https://www.readbyqxmd.com/read/28009939/comparative-efficacy-and-tolerability-of-antimuscarinic-agents-and-the-selective-%C3%AE-3-adrenoceptor-agonist-mirabegron-for-the-treatment-of-overactive-bladder-which-is-more-preferable-as-an-initial-treatment
#15
Minoru Kobayashi, Akinori Nukui, Takao Kamai
OBJECTIVES: The objective of the present study was to evaluate comparative efficacy and tolerability of antimuscarinics and mirabegron as primary and salvage therapy in patients with overactive bladder (OAB). METHODS: Patients ≥50 years with OAB symptoms were enrolled. Patients were initially treated with antimuscrinics or mirabegron for 8 weeks. When patients were refractory or intolerant to an initial treatment, drugs were switched to the other. The initial and second-line efficacy was assessed at baseline, 4 and 8 weeks after the treatment having the following parameters of the International Prostatic Symptom Score (IPSS), quality of life (QOL) index, Overactive Bladder Symptom Score (OABSS) and post-void residual (PVR) urine volume...
December 23, 2016: Lower Urinary Tract Symptoms
https://www.readbyqxmd.com/read/28008957/transurethral-enucleation-of-prostate-with-button-electrode-plasmakinetic-vaporization-for-the-treatment-of-benign-prostatic-hyperplasia
#16
Bo Peng, Jianhua Huang, Guangchun Wang, Haimin Zhang, Min Liu
Benign prostatic hyperplasia (BPH) is a common disease in aged men. In this study, we investigated the efficacy and safety of transurethral enucleation of prostate with button electrode plasmakinetic vaporization for the treatment of BPH. 60 patients diagnosed with BPH who were treated in our hospital from August to December, 2014 by enucleation with button electrode were retrospectively reviewed, and operation time, urinary catheter indwelling time, continuous bladder irrigation time, operation related complications, maximum urinary flow rate (Qmax), post-void residual urine volume (PVR), International Prostate Symptom Score (IPSS), quality of life assessment (QOL), perioperative hemoglobin and electrolytes were recorded...
December 23, 2016: Scientific Reports
https://www.readbyqxmd.com/read/28007369/pentobarbital-in-the-context-of-possible-suicides-analysis-of-a-case
#17
Paula Melo, Pedro Costa, Maria José Quintas, André Castro, Sónia Tarelho, João Miguel Franco, Helena M Teixeira
Pentobarbital is a barbiturate, acting as a central nervous system depressant (CNS), being used for its anticonvulsant, sedative, hypnotic and anaesthetic properties. Barbiturates were replaced by benzodiazepines, leading to a decrease in poisoning cases with these compounds. However, pentobarbital is still used in many countries as an anaesthetic in veterinary medicine. Due to its properties, this compound is sought after by people who wish to commit suicide, acquiring it on the black market. The authors present an unusual fatal pentobarbital intoxication case, in a 37 years-old male salesperson, with no known connection with the veterinary field, being more difficult to obtain this compound...
December 8, 2016: Forensic Science International
https://www.readbyqxmd.com/read/27995009/therapeutic-effects-of-electrical-stimulation-on-overactive-bladder-a-meta-analysis
#18
REVIEW
De Ting Zhu, Xiao Jun Feng, Yun Zhou, Jian Xian Wu
BACKGROUND: To systematically evaluate the therapeutic effect of electrical stimulation (ES) on overactive bladder (OB). METHOD: We retrieved information by searching databases from PubMed, CBM-disc, The Cochrane Library, ScienceDirect (from Elsevier publishers) and Springer publishers up to March 2016. We looked for randomized controlled trials that studied ES in OB treatment with subject headings and keywords using literature searches and manual retrieval. References of included studies were reviewed...
2016: SpringerPlus
https://www.readbyqxmd.com/read/27983781/one-shot-in-vitro-evolution-generated-an-antibody-fragment-for-testing-urinary-cotinine-with-more-than-40-fold-enhanced-affinity
#19
Hiroyuki Oyama, Izumi Morita, Yuki Kiguchi, Erika Banzono, Kasumi Ishii, Satoshi Kubo, Yoshiro Watanabe, Anna Hirai, Chiaki Kaede, Mitsuhiro Ohta, Norihiro Kobayashi
Immunoassays for cotinine, a major nicotine metabolite, in the urine are useful for monitoring the degree of tobacco smoke exposure. However, hybridoma-based anti-cotinine antibodies lack sufficient binding affinity to perform practically sensitive measurements, and thus most cotinine assays still rely on polyclonal antibodies. Here, we describe the generation of a mutant single-chain Fv fragment (scFv) that was used in an enzyme-linked immunosorbent assay (ELISA) to determine urinary cotinine levels in passive smokers...
December 16, 2016: Analytical Chemistry
https://www.readbyqxmd.com/read/27979279/molecularly-imprinted-polymer-grafted-paper-based-method-for-the-detection-of-17%C3%AE-estradiol
#20
Lei Xiao, Zhen Zhang, Chuanchen Wu, Liying Han, Hongyan Zhang
17β-estradiol (17β-E2) usually is used to promote the growth of animal. Abuse of 17β-E2 has become a global food security problem, because the residues in foods can cause endocrine disorder through the food chain. A novel molecularly imprinted polymer (MIP) grafted paper-based method for the sensitive and specific detection of 17β-E2 was reported in this work. Results showed that the MIP's optimum synthetic conditions were as follows: 12 mL of acetonitrile was chosen as the solvent; the molar ratio of template molecule, functional monomer and cross linker was 1:12:12...
April 15, 2017: Food Chemistry
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