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https://www.readbyqxmd.com/read/29765378/epithelial-c5ar1-signaling-enhances-uropathogenic-escherichia-coli-adhesion-to-human-renal-tubular-epithelial-cells
#1
Yun Song, Kun-Yi Wu, Weiju Wu, Zhao-Yang Duan, Ya-Feng Gao, Liang-Dong Zhang, Tie Chong, Malgorzata A Garstka, Wuding Zhou, Ke Li
Recent work in a murine model of ascending urinary tract infection has suggested that C5a/C5aR1 interactions play a pathogenic role in the development of renal infection through enhancement of bacterial adhesion/colonization to renal tubular epithelial cells (RTECs). In the present study, we extended these observations to human. We show that renal tubular epithelial C5aR1 signaling is involved in promoting uropathogenic Escherichia coli (UPEC) adhesion/invasion of host cells. Stimulation of primary cultures of RTEC with C5a resulted in significant increases in UPEC adhesion/invasion of the RTEC...
2018: Frontiers in Immunology
https://www.readbyqxmd.com/read/29755469/urinary-peptides-as-a-novel-source-of-t-cell-allergen-epitopes
#2
Ricardo da Silva Antunes, John Pham, Curtis McMurtrey, William H Hildebrand, Elizabeth Phillips, Simon Mallal, John Sidney, Paula Busse, Bjoern Peters, Véronique Schulten, Alessandro Sette
Mouse allergy in both laboratory workers and in inner-city children is associated with allergic rhinitis and asthma, posing a serious public health concern. Urine is a major source of mouse allergens, as mice spray urine onto their surroundings, where the proteins dry up and become airborne on dust particles. Here, we tested whether oligopeptides that are abundant in mouse urine may contribute to mouse allergic T cell response. Over 1,300 distinct oligopeptides were detected by mass spectrometry analysis of the low molecular weight filtrate fraction of mouse urine (LoMo)...
2018: Frontiers in Immunology
https://www.readbyqxmd.com/read/29753141/a-lead-analysis-based-on-amine-functionalized-bimodal-mesoporous-silica-nanoparticles-in-human-biological-samples-by-ultrasound-assisted-ionic-liquid-trap-micro-solid-phase-extraction
#3
Hamid Shirkhanloo, Sara Davari Ahranjani
A simple and applied method based on ultrasound assisted-Ionic liquid trap-micro solid phase extraction (USA-ILT-μSPE) was demonstrated for preconcentration/separation of lead (Pb) in human blood and urine samples. By proposed method, the amine-functionalized bimodal mesoporous silica nanoparticles (NH2 -UVM7 ) as solid phase sorbent was used for lead analysis in human biological samples at pH ≈ 7.0. After shaking/centrifuging of sample, the Pb (II) was extracted with NH2 -UVM7 without any chelating agents, trapped in ionic liquid phase and settled down in conical centrifuge tube as a residual solution [Pb→:NH2 -UVM7 ]...
May 4, 2018: Journal of Pharmaceutical and Biomedical Analysis
https://www.readbyqxmd.com/read/29752752/long-term-safety-and-efficacy-of-the-novel-%C3%AE-3-adrenoreceptor-agonist-vibegron-in-japanese-patients-with-overactive-bladder-a-phase-iii-prospective-study
#4
Masaki Yoshida, Hidehiro Kakizaki, Satoru Takahashi, Shinji Nagai, Takafumi Kurose
OBJECTIVES: To evaluate the long-term safety and efficacy of vibegron 50 mg and 100 mg, a novel β3 -adrenoreceptor agonist, in Japanese patients with overactive bladder. METHODS: This was a 1-year, multicenter, open-label, non-controlled study. After a 1-week observation phase, patients were treated with vibegron for 52 weeks. When the efficacy was insufficient after an 8-week treatment with 50 mg, the dose was increased to 100 mg and maintained for an additional 44 weeks...
May 11, 2018: International Journal of Urology: Official Journal of the Japanese Urological Association
https://www.readbyqxmd.com/read/29752646/mri-features-after-prostatic-artery-embolization-for-the-treatment-of-medium-and-large-volume-benign-hyperplasia
#5
Hongtao Zhang, Yanguang Shen, Jingjing Pan, Haiyi Wang, Yan Zhong, Yingwei Wang, Huiyi Ye
PURPOSE: To assess magnetic resonance imaging (MRI) features after prostatic artery embolization (PAE) for the treatment of medium- and large-volume benign prostatic hyperplasia and to correlate prostate volume with clinical indexes. METHODS: We retrospectively evaluated 28 patients who underwent PAE. MRI examinations of the prostate were performed to evaluate signal intensity changes and the characteristics of infarcted areas. Prostate volume and the apparent diffusion coefficient (ADC) were measured at an average of 10 days post-PAE and at 1, 3, 6, and 12 months post-PAE...
May 12, 2018: La Radiologia Medica
https://www.readbyqxmd.com/read/29745000/impacts-of-serum-25-oh-vitamin-d-level-on-lower-urinary-tract-symptoms-in-male-a-step-forward-to-decrease-overactive-bladder
#6
Sangjun Yoo, Sohee Oh, Hyung Suk Kim, Han Seok Choi, Juhyun Park, Sung Yong Cho, Hwancheol Son, Hyeon Jeong, Hae Won Lee, Min Chul Cho
OBJECTIVES: To evaluate the impacts of serum vitamin D level on male lower urinary tract symptoms (LUTS). MATERIALS AND METHODS: From 2014 to 2017, 434 male patients with LUTS were included. The impacts of vitamin D on LUTS were evaluated using multivariate analysis to adjust for age, body mass index, prostate specific antigen, testosterone, Hemoglobin A1c, physical activity, and prostate volume. To exclude the effect of seasons, we also analyzed the impacts during each season...
May 9, 2018: BJU International
https://www.readbyqxmd.com/read/29741118/15-years-survey-of-safety-and-efficacy-of-serenoa-repens-extract-in-benign-prostatic-hyperplasia-patients-with-risk-of-progression
#7
Andrey Zinovievich Vinarov, Leonid Grigorievich Spivak, Darina Vladimirovna Platonova, Leonid Mikhailovich Rapoport, Dmitry Olegovich Korolev
AIM: The aim of this study is to investigate the efficacy and safety of long-term therapy with Serenoa repens extract with regard to halting benign prostatic hyperplasia progression. MATERIAL AND METHODS: An open non-comparative observational study of the continuous use of S. repens plant extract at a dosage of 320 mg once a day for 15 years was performed in 30 patients at risk for benign prostatic hyperplasia progression. Changes in IPSS (International Prostatic Symptoms Scale) and QoL (Quality of life) scores and changes in Qmax, voided volume, residual urine volume, and prostate volume were evaluated during the study...
May 1, 2018: Urologia
https://www.readbyqxmd.com/read/29738807/environmental-and-biological-monitoring-on-an-oncology-ward-during-a-complete-working-week
#8
Michael Koller, Antje Böhlandt, Christopher Haberl, Dennis Nowak, Rudolf Schierl
Workplace exposure to antineoplastic drugs (AD) is still of evident concern to all occupationally exposed persons in the healthcare sector as residues in relevant concentrations continue to be present. With respect to the carcinogenic and mutagenic potential of ADs and their toxicity on reproduction, occupational exposure should be kept as low as reasonably achievable (ALARA). In the oncology patient care, the medical staff is involved both in chemotherapy administration and handling of AD-contaminated body fluids of the patients...
May 5, 2018: Toxicology Letters
https://www.readbyqxmd.com/read/29738695/an-approach-to-analytical-validation-and-testing-of-body-fluid-assays-for-the-automated-clinical-laboratory
#9
Darci R Block, Lucas J Ouverson, Craig A Wittwer, Amy K Saenger, Nikola A Baumann
BACKGROUND: Biochemical analysis of body fluids may lend insight into the pathogenesis of disease. Most commercially-available clinical laboratory methods are intended for blood-derived specimens and/or urine and laboratories must characterize the analytical performance of these methods for other specimen types such as body fluids. The aim of this work is to demonstrate one approach for characterizing analytical performance of assays for clinical analysis of body fluids. METHODS: Residual waste samples were obtained from clinically ordered testing...
May 5, 2018: Clinical Biochemistry
https://www.readbyqxmd.com/read/29738184/-correlation-between-tcm-syndrome-types-and-clinical-symptoms-of-benign-prostatic-hyperplasia-with-chronic-prostatitis
#10
Xin-Fei Huang, Ke-Qin Ning, Qing Wang, Tao Liu, Ying He, Jian-Guo Xue, Li-Qin Dai, Yong-Kang Zhu
Objective: To investigate the correlation between the syndrome types of traditional Chinese medicine (TCM) and clinical symptoms of benign prostatic hyperplasia (BPH) with chronic prostatitis (BPH-CP). METHODS: We selected 150 cases of BPH-CP in this study and divided them into 7 TCM syndrome types. Using univariate and multivariate logistic regression analyses, we studied the correlation of each TCM syndrome type with the age, disease course, prostate volume, postvoid residual urine volume (PVR), prostate-specific antigen (PSA) level, maximum urinary flow rate (Qmax), and International Prostate Symptoms Score (IPSS)...
December 2017: Zhonghua Nan Ke Xue, National Journal of Andrology
https://www.readbyqxmd.com/read/29732213/association-between-detrusor-muscle-function-and-level-of-the-spinal-cord-injury
#11
Maryam Kooshesh, Mahdi Safdarian, Abolghasem Nikfallah, Alexander R Vaccaro, Vafa Rahimi-Movaghar
Introduction: Traumatic spinal cord injury (TSCI) is among the most severe disabilities with an estimation of 2.5 million people affected worldwide. The purpose of this study was to investigate the association between detrusor muscle function and the level of the spinal cord injury. Material and methods: All patients with TSCI who underwent urodynamic evaluation at the Brain and Spinal Injury Research Center (BASIR) of Imam Khomeini hospital complex from March 2014 to March 2016 were retrospectively entered in this cross-sectional study...
2018: Central European Journal of Urology
https://www.readbyqxmd.com/read/29731093/graft-contrast-induced-nephropathy-caused-by-prerenal-transplant-computed-tomography-a-case-report
#12
S D Hwang, K-M Park, S W Lee, J-Y Han, M-J Kim, J H Song
BACKGROUND: We report a case of posttransplant contrast-induced nephropathy (CIN) that occurred after performing computed tomography (CT) scanning for pretransplant cardiac and vascular evaluation. CASE PRESENTATION: The patient had an 8-year history of hemodialysis and was admitted to the hospital for a kidney transplant from a deceased donor. Cardiac CT imaging and 3-dimensional low-extremity CT angiography were performed to confirm the patient's cardiac and iliac artery function...
May 2018: Transplantation Proceedings
https://www.readbyqxmd.com/read/29730838/thulium-vapoenucleation-of-the-prostate-versus-holmium-laser-enucleation-of-the-prostate-for-the-treatment-of-large-volume-prostates-preliminary-6-month-safety-and-efficacy-results-of-a-prospective-randomized-trial
#13
B Becker, T R W Herrmann, A J Gross, C Netsch
PURPOSE: We compared the perioperative and postoperative characteristics of thulium vapoenucleation and holmium laser enucleation of the prostate for the treatment of large volume benign prostatic hyperplasia. MATERIALS AND METHODS: A total of 94 patients with benign prostatic hyperplasia and a median prostate size of 80 (IQR 46.75-100) cc were either randomized to thulium vapoenucleation or holmium laser enucleation of the prostate. Patients were assessed preoperatively, 1 and 6 months postoperatively...
May 5, 2018: World Journal of Urology
https://www.readbyqxmd.com/read/29729702/blood-metabolism-study-on-protection-of-residual-renal-function-of-hemodialysis-patients-by-traditional-chinese-medicine-kidney-flaccidity-compound
#14
Qiong-Dan Hu, Wei-Hua Wu, Yan Zeng, Ji Wen, Xiao-Jun Li, Wei Pan, Mao-Ping Zhang, Bo Hu, Chun-Yan Lei, Junming Fan
In recent years, metabolomics using high-performance liquid chromatography (UPLC) has been used to study the metabolic profiles in plasma, urine, stool and tissue in animal model of chronic kidney disease (CKD). In the previous work, we found that traditional Chinese medicine (TCM) "Kidney Flaccidity Compound" (KFC) based on "kidney flaccidity theory" can improve renal function and quality of life of patients with kidney disease. This study aimed to investigate the metabolic profiles in peripheral blood of hemodialysis patients administrated by KFC for 1...
April 30, 2018: Cellular and Molecular Biology
https://www.readbyqxmd.com/read/29727542/-3-1-bladder-function-restoration-combined-with-holmium-laser-enucleation-of-the-prostate-for-benign-prostatic-hyperplasia-with-acontractile-detrusor
#15
Xiang Wan, Chong Liu, Huan Xu, Meng Gu, Yan-Bo Chen, Yu-Bing Peng, Qi Chen, Zhi-Kang Cai, Zhong Wang
Objective: To investigate the clinical effect of "3+1" bladder function restoration combined with holmium laser enucleation of the prostate (HoLEP) in the treatment of benign prostatic hyperplasia (BPH) with acontractile detrusor (ACD). METHODS: We treated 35 BPH patients with ACD by HoLEP followed by "3+1" bladder function restoration, that is, a 3-phase bladder function training plus simultaneous 1-drug medication after surgery. We recorded and analyzed the detrusor pressure, post-void residual urine volume (PVR), maximum urinary flow rate (Qmax), International Prognostic Scoring System (IPSS) scores, quality of life (QoL), voluntary micturition, satisfaction with the bladder function, hydronephrosis, ureterectasia, renal function, and urinary tract infection of the patients before and after treatment...
October 2017: Zhonghua Nan Ke Xue, National Journal of Andrology
https://www.readbyqxmd.com/read/29726659/-a-dosage-regimen-of-m-receptor-blocker-after-turp-for-severe-bph-with-predominant-urine-storage-symptoms
#16
Jian-Liang Cai, Da Chen, Yu-Feng Song, Jing-Chao Han, Jing-Tian Xiao, Ning-Chen Li, Ming Xia, Yan-Qun Na
Objective: To study the dosage regimen of oral M-receptor blocker following transurethral resection of the prostate (TURP) for severe benign prostate hyperplasia (BPH) with predominant urine storage period symptoms (USPSs) and its clinical effect. METHODS: Severe BPH patients with predominant USPSs received oral tolterodine (2 mg q12d or 4 mg qd) 6 hours after TURP for 4 weeks. The medication continued for another 2 weeks in case of recurrence of USPSs or until the 12th week in case of repeated recurrence...
September 2017: Zhonghua Nan Ke Xue, National Journal of Andrology
https://www.readbyqxmd.com/read/29726643/-expressions-of-interleukin-17-and-interleukin-8-in-the-prostatic-tissue-of-the-patients-with-bph-or-bph-with-inflammation
#17
Rui Gao, Qi-Xiang Liu, Hui-Liang Zhou, Lin-Sheng Cao, Tao Jiang, Song-Xi Tang, Yi-Lang Ding
Objective: To investigate the expressions of interleukin-17 (IL-17) and interleukin-8 (IL-8) in benign prostatic hyperplasia (BPH) and BPH complicated with histological inflammation and their significance. METHODS: According to the results of HE staining, we divided 60 cases of BPH treated by transurethral resection of the prostate (TURP) into a BPH group (n = 23) and a BPH with inflammation group (n = 37). We analyzed the clinical data of the patients and determined the mRNA and protein expressions of IL-17 and IL-8 by immunohistochemistry, real-time fluorescence quantitative PCR, and Western blot, respectively...
August 2017: Zhonghua Nan Ke Xue, National Journal of Andrology
https://www.readbyqxmd.com/read/29719001/optimized-application-of-the-secreted-nano-luciferase-reporter-system-using-an-affinity-purification-strategy
#18
JingZhe Li, ZhiLan Guo, Takashi Sato, Bo Yuan, YanYan Ma, Dan Qian, JuYing Zhong, MengMeng Jin, Peng Huang, LuYang Che, Yi Wang, Yan Lei, ChangZhen Liu
Secreted Nano-luciferase (secNluc) is a newly engineered secreted luciferase that possesses advantages of high structural stability, long half-life, and glow-type kinetics together with high light emission intensity, and thus would become one of the most valuable tools for bioluminescence assays. However, like other secreted luciferases, secNluc has to mix with the components in the conditioned medium surrounding test cells, or in the biological samples such as blood or urine after being secreted. These components may interfere with secNluc-catalyzed bioluminescence reactions and thus limit the application of the secNluc reporter system...
2018: PloS One
https://www.readbyqxmd.com/read/29716443/corticosteroids-in-liver-and-urine-in-sicilian-cattle-by-a-lc-ms-ms-method
#19
Vita Giaccone, Andrea Macaluso, Gaetano Cammilleri, Antonio Vella, Vincenzo Ferrantelli
The presence of corticosteroid residues was assessed in urine and liver samples from livestock of Sicily. A total of 630 bovine samples were collected from farms and slaughterhouses. The samples were analyzed using solid-phase extraction (SPE) coupled with ultra-performance liquid chromatography tandem mass spectrometry (UHPLC-MS/MS). All the corticosteroids found were under the maximum residue limit imposed by Commission Regulation (EC) 37/2010. About 4% of liver samples showed dexamethasone levels above the limit of detection (LOD), with a mean of 1...
May 2, 2018: Food Additives & Contaminants. Part B, Surveillance
https://www.readbyqxmd.com/read/29710514/pathophysiological-investigations-anxiolytic-effects-and-interaction-of-a-semisynthetic-riparin-with-benzodiazepine-receptors
#20
Éverton José Ferreira de Araújo, Luís Mário Rezende-Júnior, Layana Karine Farias Lima, Marcelo Pereira da Silva-Júnior, Oskar Almeida Silva, Benedito Pereira de Sousa Neto, Antonia Amanda Cardoso de Almeida, Stanley Juan Chavez Gutierrez, Adriana da Rocha Tomé, Luciano da Silva Lopes, Paulo Michel Pinheiro Ferreira, Francisco das Chagas Alves Lima
We have reported Riparin A as a promising antiparasitic molecule ​​against Leishmania amazonensis promastigotes. This work evaluated the acute oral toxicity of Riparin A and its anxiolytic effects using in vivo models and computational tools. Mice were submitted to acute oral toxicity tests (Guideline OECD 423). Later, anxiety assays with Riparin A (50, 100 and 200 mg/kg: elevated plus maze, light/dark box and marble burying) were performed. Theoretical calculations analyzed interaction of Riparin A with gamma-amino butyric acid (GABA) receptors...
April 24, 2018: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
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