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https://www.readbyqxmd.com/read/28342148/further-characterization-and-independent-validation-of-a-dna-aptamer-quantum-dot-based-magnetic-sandwich-assay-for-campylobacter
#1
John G Bruno, Jeffrey C Sivils
Previously reported DNA aptamers developed against surface proteins extracted from Campylobacter jejuni were further characterized by aptamer-based Western blotting and shown to bind epitopes on proteins weighing ~16 and 60 kD from reduced C. jejuni and Campylobacter coli lysates. Proteins of these approximate weights have also been identified in traditional antibody-based Western blots of Campylobacter spp. Specificity of the capture and reporter aptamers from the previous report was further validated by aptamer-based ELISA-like (ELASA) colorimetric microplate assay...
March 24, 2017: Folia Microbiologica
https://www.readbyqxmd.com/read/28337234/is-computerised-tomography-better-than-fibreoptic-gastroscopy-for-early-detection-of-gastric-varices
#2
Burak Suvak, Murat Kekilli, Yavuz Beyazıt, Sarper Okten, Alpaslan Tanoglu, Nurgul Sasmaz
INTRODUCTION: Video endoscopic diagnosis of gastric varices is particularly limited, owing to the deep submucosal or subserosal location of the varices and the normal appearance of the overlying mucosa. AIM: We present and emphasise the value of computerised tomography (CT) examination in the early detection of gastric varices (GVs). MATERIAL AND METHODS: In this retrospective study, a total of 216 consecutive patients with cirrhosis were evaluated at the Turkiye Yuksek Ihtisas Training and Research Hospital between September 2008 and March 2011...
2017: Przegla̜d Gastroenterologiczny
https://www.readbyqxmd.com/read/28328241/perfusion-ct-in-acute-stroke-effectiveness-of-automatically-generated-colour-maps
#3
Maja Ukmar, Ferruccio Degrassi, Roberta Antea Pozzi Mucelli, Francesca Neri, Fabio Pozzi Mucelli, Maria Assunta Cova
OBJECTIVE: To evaluate the accuracy of perfusion CT (pCT) in the definition of the infarcted core and the penumbra, comparing the data obtained from the evaluation of parametric maps [cerebral blood volume (CBV), cerebral blood flow (CBF) and mean transit time (MTT)] with software-generated colour maps. METHODS: A retrospective analysis was performed to identify patients with suspected acute ischaemic strokes and who had undergone unenhanced CT and pCT carried out within 4...
April 2017: British Journal of Radiology
https://www.readbyqxmd.com/read/28327901/data-resources-for-the-identification-and-interpretation-of-actionable-mutations-by-clinicians
#4
A Prawira, T J Pugh, T L Stockley, L L Siu
Following initial characterization of the reference human genome, initiatives have evolved worldwide to identify genomic aberrations in cancer with the aim of deriving diagnostic, prognostic and predictive information. However, the functional and clinical relevance of many somatic variants in cancer are presently unknown and there is no consensus definition of "actionability" for genomic aberrancies. Therefore, while robust detection of a variety of genetic aberrations in clinical specimens remains a technical hurdle, the greater challenge lies in the interpretation of these alterations...
January 24, 2017: Annals of Oncology: Official Journal of the European Society for Medical Oncology
https://www.readbyqxmd.com/read/28327364/telomere-length-analysis-in-down-syndrome-birth
#5
Pranami Bhaumik, Mandar Bhattacharya, Priyanka Ghosh, Sujoy Ghosh, Subrata Kumar Dey
Human reproductive fitness depends upon telomere chemistry. Maternal age, meiotic nondisjunction error and telomere length of mother of trisomic child are someway associated. Reports exhibiting maternal inheritance of telomere length in Down syndrome child are very scanty. To investigate this, we collected peripheral blood from 170 mothers of Down syndrome child and 186 age matched mothers of euploid child with their newly born babies. Telomere length was measured by restriction digestion - southern blotting technique...
March 19, 2017: Mechanisms of Ageing and Development
https://www.readbyqxmd.com/read/28324755/sedentary-behavior-different-types-of-operationalization-influence-outcome-measures
#6
Malou H J Fanchamps, Hendrika J G van den Berg-Emons, Henk J Stam, Johannes B J Bussmann
INTRODUCTION: Sedentary behavior (SB) influences health status independently of physical activity. The formal definition of SB is: "any waking behavior characterized by an energy expenditure ≤1.5 METs while in a sitting or reclining posture". However, measuring SB mostly does not include both the intensity and postural component. The aim of this study was to quantify the effect of type of operationalization of SB on total sedentary time and the pattern of SB. METHODS: 53 healthy subjects were measured 24h with a multi-sensor activity monitor that provides a valid one-second detection of body postures and movements and a calculated intensity measure...
March 6, 2017: Gait & Posture
https://www.readbyqxmd.com/read/28323622/a-quality-of-life-assessment-and-the-correlation-between-generic-and-disease-specific-questionnaires-scores-in-outpatients-with-chronic-liver-disease-pilot-study
#7
Milica Obradovic, Zoran Gluvic, Nina Petrovic, Milan Obradovic, Ratko Tomasevic, Predrag Dugalic, Esma R Isenovic
INTRODUCTION: Chronic liver diseases (CLD) are important cause of morbidity and mortality in general population. The aim of this study was to analyze potential differences between patients with CLD and healthy control group, and to estimate the severity of CLD by using simple questionnaires: general health questionnaire (GHQ-12) and chronic liver disease questionnaire (CLDQ). METHODS: A cross-sectional pilot study was performed in Zemun Clinical Hospital during years 2014 and 2015...
March 20, 2017: Romanian Journal of Internal Medicine, Revue Roumaine de Médecine Interne
https://www.readbyqxmd.com/read/28322735/stella-collaborates-in-distinct-mesendodermal-cell-subpopulations-at-the-fetal-placental-interface-in-the-mouse-gastrula
#8
Adam D Wolfe, Adriana M Rodriguez, Karen M Downs
The allantois-derived umbilical component of the chorio-allantoic placenta shuttles fetal blood to and from the chorion, thereby ensuring fetal-maternal exchange. The progenitor populations that establish and supply the fetal-umbilical interface lie, in part, within the base of the allantois, where the germ line is claimed to segregate from the soma. Results of recent studies in the mouse have reported that STELLA (DPPA-3, PGC7) co-localizes with PRDM1 (BLIMP1), the bimolecular signature of putative primordial germ cells (PGCs) throughout the fetal-placental interface...
March 16, 2017: Developmental Biology
https://www.readbyqxmd.com/read/28317477/circulating-biomarkers-of-collagen-metabolism-and-prognosis-of-heart-failure-with-reduced-or-mid-range-ejection-fraction
#9
Joana Moura Ferreira, Sara Moura Ferreira, Maria João Ferreira, Inês Falcão-Pires
BACKGROUND: Change in the architecture and composition of cardiac extracellular matrix is a key element in adverse cardiac remodeling that occurs during heart failure. Most of the times, the result is the development of fibrosis, which has a huge impact on the pathophysiology and clinical outcomes of heart failure syndromes. Several molecules related to collagen metabolism detectable in the blood have been proposed as biomarkers of myocardial fibrosis. Despite concerns regarding the true ability of these biomarkers to reflect quantitative and qualitative changes in myocardial collagen, a growing body of evidence suggests that they may play a potential role in several aspects of heart failure management...
March 17, 2017: Current Pharmaceutical Design
https://www.readbyqxmd.com/read/28315696/auditory-deficits-in-amusia-extend-beyond-poor-pitch-perception
#10
Kelly L Whiteford, Andrew J Oxenham
Congenital amusia is a music perception disorder believed to reflect a deficit in fine-grained pitch perception and/or short-term or working memory for pitch. Because most measures of pitch perception include memory and segmentation components, it has been difficult to determine the true extent of pitch processing deficits in amusia. It is also unclear whether pitch deficits persist at frequencies beyond the range of musical pitch. To address these questions, experiments were conducted with amusics and matched controls, manipulating both the stimuli and the task demands...
March 16, 2017: Neuropsychologia
https://www.readbyqxmd.com/read/28315218/use-of-humanized-rs-atl8-reporter-system-for-detection-of-allergen-specific-ige-sensitization-in-human-food-allergy
#11
Eman Ali Ali, Ryosuke Nakamura, Franco H Falcone
Allergen-specific Immunoglobulin E (IgE) determination lies at the heart of diagnosis of sensitization to food and other allergens. In the past few years, reporter systems capable of detecting the presence of allergen-specific IgE have been developed by several labs. These rely on humanized rat basophil leukemia cell lines stably transfected with reporter genes such as firefly luciferase. In this chapter, we describe protocols for the use of the RS-ATL8 cell line (IgE cross-linking-induced luciferase expression; EXiLE) in 96-well and 384-well formats...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28306248/temperature-sensing-using-thermal-population-of-low-lying-energy-levels-with-sm0-01gd0-99-vo4
#12
Jiajia Cai, Lu Zhao, Fangfang Hu, Xiantao Wei, Yonghu Chen, Min Yin, Chang-Kui Duan
A temperature sensing scheme is proposed that is based on the dramatic temperature dependence of the photoluminescence when Sm(3+) dopants are excited from thermally populated (6)H7/2,9/2 levels, rather than the ground level (6)H5/2, to the (4)G5/2 luminescent level. The scheme has the advantage of eliminating laser heating and background Stokes-type scattering noise. Experimental realization was carried out on a (Sm0.01Gd0.99)VO4 sample by detecting the intensities at 550-580 nm using excitation wavelengths of 601...
March 17, 2017: Inorganic Chemistry
https://www.readbyqxmd.com/read/28306142/the-effect-of-smoking-on-inflammatory-and-bone-remodeling-markers-in-gingival-crevicular-fluid-and-subgingival-microbiota-following-periodontal-therapy
#13
D F Bunaes, M Mustafa, H G Mohamed, S A Lie, K N Leknes
BACKGROUND AND OBJECTIVE: Periodontal health is mediated by suppressing microorganisms inducing a local inflammatory host response. Smoking may impair this process. This study compares gingival crevicular fluid levels of inflammatory and bone remodeling markers in heavy smokers and non-smokers following active and supportive periodontal therapy in patients with chronic periodontitis. MATERIAL AND METHODS: Gingival crevicular fluid and subgingival plaque were collected from the deepest periodontal pocket in 50 patients, 25 smokers and 25 non-smokers, at baseline (T0), following active (T1) and 12 mo of supportive periodontal therapy (T2)...
March 17, 2017: Journal of Periodontal Research
https://www.readbyqxmd.com/read/28303334/an-insect-inspired-model-for-visual-binding-ii-functional-analysis-and-visual-attention
#14
Brandon D Northcutt, Charles M Higgins
We have developed a neural network model capable of performing visual binding inspired by neuronal circuitry in the optic glomeruli of flies: a brain area that lies just downstream of the optic lobes where early visual processing is performed. This visual binding model is able to detect objects in dynamic image sequences and bind together their respective characteristic visual features-such as color, motion, and orientation-by taking advantage of their common temporal fluctuations. Visual binding is represented in the form of an inhibitory weight matrix which learns over time which features originate from a given visual object...
March 16, 2017: Biological Cybernetics
https://www.readbyqxmd.com/read/28302888/investigation-of-productivity-in-a-south-indian-malabari-goat-herd-shows-opportunities-for-planned-animal-health-management-to-improve-food-security
#15
N D Sargison, S A J Ivil, J Abraham, S P S Abubaker, A M Hopker, S Mazeri, I A Otter, N Otter
Here the authors report the objective veterinary clinical measurement of productivity in a representative south Indian Malabari goat herd. The authors show failure to meet pragmatic production targets that are commensurate with the animals' genetic potential or adequate to meet the demands of global food security. The authors suggest that this situation may have arisen as a consequence of animal husbandry constraints and protein undernutrition and imply the involvement of nematode parasitism. Benzimidazole resistance was detected in Haemonchus species, showing the need for better understanding of the principles of sustainable helminth parasite control within the southern Indian context...
March 18, 2017: Veterinary Record
https://www.readbyqxmd.com/read/28293109/effects-of-a-structured-20-session-slow-cortical-potential-based-neurofeedback-program-on-attentional-performance-in-children-and-adolescents-with-attention-deficit-hyperactivity-disorder-retrospective-analysis-of-an-open-label-pilot-approach-and-6-month-follow
#16
Johanna S Albrecht, Sarah Bubenzer-Busch, Anne Gallien, Eva Lotte Knospe, Tilman J Gaber, Florian D Zepf
OBJECTIVE: The aim of this approach was to conduct a structured electroencephalography-based neurofeedback training program for children and adolescents with attention-deficit hyperactivity disorder (ADHD) using slow cortical potentials with an intensive first (almost daily sessions) and second phase of training (two sessions per week) and to assess aspects of attentional performance. PATIENTS AND METHODS: A total of 24 young patients with ADHD participated in the 20-session training program...
2017: Neuropsychiatric Disease and Treatment
https://www.readbyqxmd.com/read/28290127/jumping-the-gun-faster-response-latencies-to-deceptive-questions-in-a-realistic-scenario
#17
Tessa Mapala, Lara Warmelink, Sally A Linkenauger
Most theories of lie detection assume that lying increases cognitive load, resulting in longer response latencies during questioning. However, the studies supporting this theory are typically laboratory-based, in settings with no specific validity in security contexts. Consequently, using virtual reality (VR), we investigated how response latencies were influenced in an ecologically valid environment of interest to security professionals. In a highly realistic airport security terminal presented in VR, a security officer asked participants yes/no questions about their belongings...
March 13, 2017: Psychonomic Bulletin & Review
https://www.readbyqxmd.com/read/28286563/antispasmodic-drugs-in-colonoscopy-a-review-of-their-pharmacology-safety-and-efficacy-in-improving-polyp-detection-and-related-outcomes
#18
REVIEW
Santosh Sanagapalli, Kriti Agnihotri, Rupert Leong, Crispin John Corte
Antispasmodic drugs are cheap, effective and generally safe. They may improve outcomes in colonoscopy, however their use has not been consistent or widespread. This manuscript reviews the three most commonly used antispasmodics in colonoscopy, namely, hyoscine butylbromide (and related ammonium compounds), glucagon and peppermint oil. The pharmacology, action and safety of the agents, as well as the evidence for them improving colonoscopic outcomes will be discussed. In addition to polyp detection, other colonoscopic outcome endpoints of interest include cecal and ileal intubation, and patient comfort...
January 2017: Therapeutic Advances in Gastroenterology
https://www.readbyqxmd.com/read/28283040/functional-variation-in-allelic-methylomes-underscores-a-strong-genetic-contribution-and-reveals-novel-epigenetic-alterations-in-the-human-epigenome
#19
Warren A Cheung, Xiaojian Shao, Andréanne Morin, Valérie Siroux, Tony Kwan, Bing Ge, Dylan Aïssi, Lu Chen, Louella Vasquez, Fiona Allum, Frédéric Guénard, Emmanuelle Bouzigon, Marie-Michelle Simon, Elodie Boulier, Adriana Redensek, Stephen Watt, Avik Datta, Laura Clarke, Paul Flicek, Daniel Mead, Dirk S Paul, Stephan Beck, Guillaume Bourque, Mark Lathrop, André Tchernof, Marie-Claude Vohl, Florence Demenais, Isabelle Pin, Kate Downes, Hendrick G Stunnenberg, Nicole Soranzo, Tomi Pastinen, Elin Grundberg
BACKGROUND: The functional impact of genetic variation has been extensively surveyed, revealing that genetic changes correlated to phenotypes lie mostly in non-coding genomic regions. Studies have linked allele-specific genetic changes to gene expression, DNA methylation, and histone marks but these investigations have only been carried out in a limited set of samples. RESULTS: We describe a large-scale coordinated study of allelic and non-allelic effects on DNA methylation, histone mark deposition, and gene expression, detecting the interrelations between epigenetic and functional features at unprecedented resolution...
March 10, 2017: Genome Biology
https://www.readbyqxmd.com/read/28281689/an-innovative-application-of-time-domain-spectroscopy-on-localized-surface-plasmon-resonance-sensing
#20
Meng-Chi Li, Ying-Feng Chang, Huai-Yi Wang, Yu-Xen Lin, Chien-Cheng Kuo, Ja-An Annie Ho, Cheng-Chung Lee, Li-Chen Su
White-light scanning interferometry (WLSI) is often used to study the surface profiles and properties of thin films because the strength of the technique lies in its ability to provide fast and high resolution measurements. An innovative attempt is made in this paper to apply WLSI as a time-domain spectroscopic system for localized surface plasmon resonance (LSPR) sensing. A WLSI-based spectrometer is constructed with a breadboard of WLSI in combination with a spectral centroid algorithm for noise reduction and performance improvement...
March 10, 2017: Scientific Reports
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