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https://www.readbyqxmd.com/read/29147003/real-time-wash-free-detection-of-unlabeled-pna-dna-hybridization-using-discrete-fet-sensor
#1
Matti Kaisti, Anssi Kerko, Eero Aarikka, Petri Saviranta, Zhanna Boeva, Tero Soukka, Ari Lehmusvuori
We demonstrate an electrochemical sensor for detection of unlabeled single-stranded DNA using peptide nucleic acid (PNA) probes coupled to the field-effect transistor (FET) gate. The label-free detection relies on the intrinsic charge of the DNA backbone. Similar detection schemes have mainly concentrated on sensitivity improvement with an emphasis on new sensor structures. Our approach focuses on using an extended-gate that separates the FET and the sensing electrode yielding a simple and mass fabricable device...
November 16, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29140167/intra-tester-reliability-and-construct-validity-of-a-hip-abductor-eccentric-strength-test
#2
Richard A Brindle, D David Ebaugh, Clare E Milner
CONTEXT: Side-lying hip abductor strength tests are commonly used to evaluate muscle strength. In a 'break' test the tester applies sufficient force to lower the limb to the table while the patient resists. The peak force is postulated to occur while the leg is lowering, thus representing the participant's eccentric muscle strength. However, it is unclear whether peak force occurs before or after the leg begins to lower. OBJECTIVES: To determine intra-rater reliability and construct validity of a hip abductor eccentric strength test...
November 15, 2017: Journal of Sport Rehabilitation
https://www.readbyqxmd.com/read/29133038/new-metalo-therapeutics-of-nsaids-against-human-breast-cancer-cells
#3
Christina N Banti, Constantina Papatriantafyllopoulou, Anastasios J Tasiopoulos, Sotiris K Hadjikakou
The non steroidal anti-inflammatory drugs (NSAID's)-silver(I) metallodrugs of aspirin (aspH), salicylic acid (salH2), naproxen (napH) acid or p-hydrobenzoic acid (pHbzaH) and the mitochondriotropic triphenylarsine (tpAs) with the formulae [Ag(asp)(tpAs)3] (1), [Ag(salH)(tpAs)3] (2), [Ag(nap)(tpAs)3] (3) and {[Ag(pHbza)(tpAs)3]∙(dmf)} (4) and [Ag(tpAs)3(NO3)] (5) have been synthesized and characterized by m.p., FT-IR, UV-vis and (1)H NMR, spectroscopic techniques and X-ray crystallography. The in vitro cytotoxic activity of 1-5 against human breast adenocarcinoma cancer cells: MCF-7 (positive to estrogen receptors (ERs)) and MDA-MB-231 (negative to estrogen receptors (ERs)) was evaluated...
November 10, 2017: European Journal of Medicinal Chemistry
https://www.readbyqxmd.com/read/29132873/hpv-genotype-profile-in-a-norwegian-cohort-with-asc-us-and-lsil-cytology-with-three-year-cumulative-risk-of-high-grade-cervical-neoplasia
#4
A K Lie, A Tropé, G B Skare, T Bjørge, C M Jonassen, K Brusegard, S Lönnberg
OBJECTIVE: To explore the HPVgenotype profile in Norwegian women with ASC-US/LSIL cytology and the subsequent risk of high-grade cervical neoplasia (CIN 3+). METHODS: In this observational study delayed triage of ASC-US/LSIL of 6058 women were included from 2005 to 2010. High-risk HPV detection with Hybrid Capture 2 (HC2) was used and the HC2+ cases were genotyped with in-house nmPCR. Women were followed-up for histologically confirmed CIN3+ within three years of index HPV test by linkage to the screening databases at the Cancer Registry of Norway...
November 11, 2017: Gynecologic Oncology
https://www.readbyqxmd.com/read/29132760/hepatitis-c-in-sub-saharan-africa-the-current-status-and-recommendations-for-achieving-elimination-by-2030
#5
REVIEW
Mark W Sonderup, Mary Afihene, Reidwaan Ally, Betty Apica, Yaw Awuku, Lina Cunha, Geoffrey Dusheiko, Neliswa Gogela, Marie-Jeanne Lohouès-Kouacou, Phillip Lam, Olufunmilayo Lesi, Papa Saliou Mbaye, Emmanuel Musabeyezu, Betty Musau, Olesegun Ojo, John Rwegasha, Barbara Scholz, Abate B Shewaye, Christian Tzeuton, Chris Kassianides, C Wendy Spearman
In 2016, WHO adopted a strategy for the elimination of viral hepatitis by 2030. Africa, and more specifically, sub-Saharan Africa, carries a substantial portion of the global burden of viral hepatitis, especially chronic hepatitis B and hepatitis C virus infections. The task that lies ahead for sub-Saharan Africa to achieve elimination is substantial, but not insurmountable. Major developments in the management of hepatitis C have put elimination within reach, but several difficulties will need to be navigated on the path to elimination...
December 2017: Lancet. Gastroenterology & Hepatology
https://www.readbyqxmd.com/read/29130370/normal-adult-and-adolescent-performance-on-tasit-s-a-short-version-of-the-assessment-of-social-inference-test
#6
Skye McDonald, Cynthia Honan, Samantha K Allen, Rebecca El-Helou, Michelle Kelly, Fiona Kumfor, Olivier Piguet, Jessica L Hazelton, Christine Padgett, Hannah A D Keage
There is a need for short, reliable, sensitive assessment tools to measure social cognition. The Awareness of Social Inference Test (TASIT) is an ecologically valid instrument that uses videos of actors engaged in everyday conversations to assess emotion perception, the ability to detect lies, sarcasm and sincerity, and the ability to judge what others are thinking, intending, feeling, and saying. A recently developed short version of TASIT retains the structure of the original test and its clinical sensitivity...
November 13, 2017: Clinical Neuropsychologist
https://www.readbyqxmd.com/read/29126896/the-continuous-challenge-of-af-ablation-from-foci-to-rotational-activity
#7
REVIEW
Sanjiv M Narayan, Mohan N Vishwanathan, Christopher A B Kowalewski, Tina Baykaner, Miguel Rodrigo, Junaid A B Zaman, Paul J Wang
Pulmonary vein isolation (PVI) is central to ablation approaches for atrial fibrillation (AF), yet many patients still have arrhythmia recurrence after one or more procedures despite the latest technology for PVI. Ablation of rotational or focal sources for AF, which lie outside the pulmonary veins in many patients, is a practical approach that has been shown to improve success by many groups. Localized sources lie in atrial regions shown mechanistically to sustain AF in optical mapping and clinical studies of human AF, as well as computational and animal studies...
November 7, 2017: Portuguese Journal of Cardiology: An Official Journal of the Portuguese Society of Cardiology
https://www.readbyqxmd.com/read/29118123/estimating-initial-viral-levels-during-hiv-siv-reactivation-from-latency
#8
Mykola Pinkevych, Christine M Fennessey, Deborah Cromer, Martin Tolstrup, Ole S Søgaard, Thomas A Rasmussen, Brandon F Keele, Miles P Davenport
HIV viremia rebounds rapidly after treatment interruption, and a variety of strategies are being explored to reduce or control viral reactivation post-treatment. This viral rebound arises from reactivation of individual latently infected cells, which spread during ongoing rounds of productive infection. The level of virus produced by the initial individual reactivating cells is not known, although it may have major implications for the ability of different immune interventions to control viral rebound. Here we use data from both HIV and SIV treatment interruption studies to estimate the initial viral load post-interruption and thereby the initial individual reactivation event...
November 8, 2017: Journal of Virology
https://www.readbyqxmd.com/read/29113033/association-between-lameness-and-indicators-of-dairy-cow-welfare-based-on-locomotion-scoring-body-and-hock-condition-leg-hygiene-and-lying-behavior
#9
REVIEW
Mohammed B Sadiq, Siti Z Ramanoon, Wan Mastura Shaik Mossadeq, Rozaihan Mansor, Sharifah Salmah Syed-Hussain
Dairy cow welfare is an important consideration for optimal production in the dairy industry. Lameness affects the welfare of dairy herds by limiting productivity. Whilst the application of LS systems helps in identifying lame cows, the technique meets with certain constraints, ranging from the detection of mild gait changes to on-farm practical applications. Recent studies have shown that certain animal-based measures considered in welfare assessment, such as body condition, hock condition and leg hygiene, are associated with lameness in dairy cows...
November 5, 2017: Animals: An Open Access Journal From MDPI
https://www.readbyqxmd.com/read/29105339/prognostic-value-of-cd133-cd54-cd44-circulating-tumor-cells-in-colorectal-cancer-with-liver-metastasis
#10
Chao Fang, Chuanwen Fan, Cun Wang, Qiaorong Huang, Wentong Meng, Yongyang Yu, Lie Yang, Jiankun Hu, Yuan Li, Xianming Mo, Zongguang Zhou
In the previous study, we had showed the expression of CD133(+) CD54(+) CD44(+) cellular subpopulation of circulating tumor cells (CTCs) was significantly associated with liver metastasis of colorectal cancer (CRC). This study aimed to explore whether this subpopulation of CTCs have a prognostic value in CRC patients. Flow cytometry was used to detect the expression of cellular subpopulations of CTCs with CD133, CD54, and CD44 in 152 CRC patients, between December 2013 and October 2014. The impact of clinicopathological factors and the expression of cellular subpopulations of CTCs on overall survival were then analyzed...
November 3, 2017: Cancer Medicine
https://www.readbyqxmd.com/read/29105225/is-the-self-reporting-of-failed-back-surgery-syndrome-patients-treated-with-spinal-cord-stimulation-in-line-with-objective-measurements
#11
Lisa Goudman, Iris Smet, Peter Mariën, Mats De Jaeger, Sander De Groote, Eva Huysmans, Koen Putman, Jean-Pierre Van Buyten, Ronald Buyl, Maarten Moens
OBJECTIVES: To understand the subjective pain experience of patients, healthcare providers rely heavily on self-reporting. However, to quantify this unique pain experience, objective parameters are not yet available in daily clinical practice. With regard to patients with failed back surgery syndrome (FBSS) treated with spinal cord stimulation (SCS), pain therapists may recover the individual functional information about the patient's posture from the implantable pulse generator (IPG) of the stimulator...
November 3, 2017: Neuromodulation: Journal of the International Neuromodulation Society
https://www.readbyqxmd.com/read/29104709/paper-based-assays-for-urine-analysis
#12
REVIEW
Eric Lepowsky, Fariba Ghaderinezhad, Stephanie Knowlton, Savas Tasoglu
A transformation of the healthcare industry is necessary and imminent: hospital-centered, reactive care will soon give way to proactive, person-centered care which focuses on individuals' well-being. However, this transition will only be made possible through scientific innovation. Next-generation technologies will be the key to developing affordable and accessible care, while also lowering the costs of healthcare. A promising solution to this challenge is low-cost continuous health monitoring; this approach allows for effective screening, analysis, and diagnosis and facilitates proactive medical intervention...
September 2017: Biomicrofluidics
https://www.readbyqxmd.com/read/29100742/outcome-of-neoadjuvant-chemoradiation-in-mri-staged-locally-advanced-rectal-cancer-retrospective-analysis-of-123-chinese-patients
#13
Shing Fung Lee, Chi Leung Chiang, Francis Ann Shing Lee, Yiu Wah Wong, Chi Ming Poon, Frank Chi Sing Wong, Stewart Yuk Tung
BACKGROUND: For advanced rectal cancer with involved or threatened mesorectal fascia (MRF), current standard is pre-operative long course chemoradiotherapy (PLCRT) with either capecitabine or 5-fluorouracil (5-FU). However, few Chinese data on its clinical outcome are available, especially for those with pelvic MRI staging. METHODS: Between Jan-2009 and Oct-2014, 123 consecutive patients with biopsy proven adenocarcinoma of rectum, all with pelvic MRI staging, selected for PLCRT after multi-disciplinary team discussion were recruited...
November 1, 2017: Journal of the Formosan Medical Association, Taiwan Yi Zhi
https://www.readbyqxmd.com/read/29097317/brain-microstructure-by-multi-modal-mri-is-the-whole-greater-than-the-sum-of-its-parts
#14
Mara Cercignani, Samira Bouyagoub
The MRI signal is dependent upon a number of sub-voxel properties of tissue, which makes it potentially able to detect changes occurring at a scale much smaller than the image resolution. This "microstructural imaging" has become one of the main branches of quantitative MRI. Despite the exciting promise of unique insight beyond the resolution of the acquired images, its widespread application is limited by the relatively modest ability of each microstructural imaging technique to distinguish between differing microscopic substrates...
October 26, 2017: NeuroImage
https://www.readbyqxmd.com/read/29091611/oblique-incidence-reflectivity-difference-application-for-morphology-detection
#15
Honglei Zhan, Kun Zhao, Huibin Lü, Kuijuan Jin, Guozhen Yang, Xiaohong Chen
Analogous with scanning electron microscopy, we use an oblique-incidence reflectivity difference (OIRD) approach for morphology detection. By scanning the active carbon clusters in a one-dimensional way and the reservoir rocks in a two-dimensional way, the morphology of the samples' surface can be revealed in OIRD signal images. High OIRD signals of active carbon samples refer to the centralized distribution areas of carbon, and the fluctuations are caused by the uneven distribution of carbon pellets. OIRD intensity is proportional to the thickness of materials...
October 20, 2017: Applied Optics
https://www.readbyqxmd.com/read/29091396/donor-acceptor-conjugated-macrocycles-synthesis-and-host-guest-coassembly-with-fullerene-toward-photovoltaic-application
#16
Si-Qi Zhang, Zhen-Yu Liu, Wei-Fei Fu, Feng Liu, Chuan-Ming Wang, Chun-Qi Sheng, Yi-Fei Wang, Ke Deng, Qing-Dao Zeng, Li-Jin Shu, Jun-Hua Wan, Hong-Zheng Chen, Thomas P Russell
Electron-rich (donor) and electron-deficient (acceptor) units to construct donor-acceptor (D-A) conjugated macrocycles were investigated to elucidate their interactions with electron-deficient fullerene. Triphenylamine and 4,7-bisthienyl-2,1,3-benzothiadiazole were alternately linked through acetylene, as the donor and acceptor units, respectively, for pentagonal 3B2A and hexagonal 4B2A macrocycles. As detected by scanning tunneling microscopy, both D-A macrocycles were found to form an interesting concentration-controlled nanoporous monolayer on highly oriented pyrolytic graphite, which could effectively capture fullerene...
November 15, 2017: ACS Nano
https://www.readbyqxmd.com/read/29090118/the-risks-of-renal-angiomyolipoma-reviewing-the-evidence
#17
REVIEW
Raouf M Seyam, Waleed K Alkhudair, Said A Kattan, Mohamed F Alotaibi, Hassan M Alzahrani, Waleed M Altaweel
Renal angiomyolipoma (RAML), though a rare benign tumor, may impose a significant morbidity or even mortality due to its unique characteristics and the complications subsequent to its treatment. The classic tumor variant is composed of smooth muscular, vascular, and fatty components. The most straightforward diagnosis is when the fat component is abundant and gives a characteristic appearance on different imaging studies. In fat-poor lesions, however, the diagnosis is difficult and presumed a renal cell carcinoma...
2017: Journal of Kidney Cancer and VHL
https://www.readbyqxmd.com/read/29081918/seroprevalence-and-risk-factors-for-peste-des-petits-ruminants-and-selected-differential-diagnosis-in-sheep-and-goats-in-tanzania
#18
Emeli Torsson, Mikael Berg, Gerald Misinzo, Ida Herbe, Tebogo Kgotlele, Malin Päärni, Nils Roos, Anne-Lie Blomström, Karl Ståhl, Jonas Johansson Wensman
Introduction: Livestock husbandry is critical for food security and poverty reduction in a low-income country like Tanzania. Infectious disease is one of the major constraints reducing the productivity in this sector. Peste des petits ruminants (PPR) is one of the most important diseases affecting small ruminants, but other infectious diseases may also be present. Objective: The objective of this study was to determine the seroprevalence and risk factors for exposure to PPR, contagious caprine pleuropneumonia (CCPP), foot-and-mouth disease (FMD), bluetongue (BT), and bovine viral diarrhoea (BVD) in sheep and goats in Tanzania...
2017: Infection Ecology & Epidemiology
https://www.readbyqxmd.com/read/29080474/v4-shape-features-for-contour-representation-and-object-detection
#19
Hui Wei, Zheng Dong, Luping Wang
Cortical area V4 lies in the middle of the visual pathway involved with object recognition. Neurons in V4 selectively respond to different curve fragments along the object contour. In this paper, we propose a computational model that captures the shape features extracted by V4 neurons. The computational model emulated the information processing mechanism in the visual cortex. It extracted curve segments that V4 neurons respond to and quantitatively represented features of the curve segments. The proposed V4 shape features could describe object contours accurately and efficiently...
September 28, 2017: Neural Networks: the Official Journal of the International Neural Network Society
https://www.readbyqxmd.com/read/29079984/pollution-source-localization-in-an-urban-water-supply-network-based-on-dynamic-water-demand
#20
Xuesong Yan, Zhixin Zhu, Tian Li
Urban water supply networks are susceptible to intentional, accidental chemical, and biological pollution, which pose a threat to the health of consumers. In recent years, drinking-water pollution incidents have occurred frequently, seriously endangering social stability and security. The real-time monitoring for water quality can be effectively implemented by placing sensors in the water supply network. However, locating the source of pollution through the data detection obtained by water quality sensors is a challenging problem...
October 27, 2017: Environmental Science and Pollution Research International
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