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Glioma AND exosome

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https://www.readbyqxmd.com/read/28583903/interleukin-13-conjugated-quantum-dots-for-identification-of-glioma-initiating-cells-and-their-extracellular-vesicles
#1
A B Madhankumar, Oliver Mrowczynski, Suhag Patel, Cody Weston, Brad Zacharia, Michael Glantz, Christopher Siedlecki, Lichong Xu, James R Connor
Cadmium selenide (CdSe) based quantum dots modified with polyethylene glycol and chemically linked to interleukin-13 (IL13) were prepared with the aim of identifying the high affinity receptor (IL13Rα2) which is expressed in glioma stem cells and exosomes secreted by these cancer stem cells. IL13 conjugated quantum dots (IL13QD) were thoroughly characterized for their physicochemical properties including particle size and surface morphology. Furthermore, the specific binding of the IL13QD to glioma cells and to glioma stem cells (GSC) was verified using a competitive binding study...
June 2, 2017: Acta Biomaterialia
https://www.readbyqxmd.com/read/28574310/exosomes-as-a-biomarker-platform-for-detecting-epidermal-growth-factor-receptor-positive-high-grade-gliomas
#2
Sasidhar Venkata Manda, Yogesh Kataria, Babul Reddy Tatireddy, Balasubramaniam Ramakrishnan, Boola Gnana Ratnam, Rahul Lath, Alok Ranjan, Amitava Ray
OBJECTIVE High-grade glial brain tumors are often characterized by an elevated expression of the tumorigenic epidermal growth factor receptor variant III ( EGFRvIII). The authors sought to establish a clinically adaptive protocol as a noninvasive diagnostic tool for EGFRvIII detection through serum exosomes. METHODS Purity of serum exosome/RNA was confirmed by electron microscopy and flow cytometry and through an RNA bioanalyzer profile. EGFRvIII amplification was initially established by semiquantitative polymerase chain reaction in tumor tissues and exosomes...
June 2, 2017: Journal of Neurosurgery
https://www.readbyqxmd.com/read/28410224/glioma-stem-cells-derived-exosomes-promote-the-angiogenic-ability-of-endothelial-cells-through-mir-21-vegf-signal
#3
Xu Sun, Xiaotang Ma, Jinju Wang, Yuhui Zhao, Yue Wang, Ji C Bihl, Yanfang Chen, Chuanlu Jiang
Glioma stem cells (GSCs) play an important role in glioblastoma prognosis. Exosomes (EXs) mediate cell communication by delivering microRNAs (miRs). Glioblastoma has a high level of miR-21 which could upregulate vascular endothelial growth factor (VEGF) expression. We hypothesized GSC-EXs can promote the angiogenic ability of endothelial cells (ECs) through miR-21/VEGF signal. GSCs were isolated from U-251 cells with stem cell marker CD133. GSCs transfected without or with scramble or miR-21 mimics were used to produce GSC-EXscon, GSC-EXssc and GSC-EXsmiR-21...
May 30, 2017: Oncotarget
https://www.readbyqxmd.com/read/28358371/cell-based-therapy-using-mir-302-367-expressing-cells-represses-glioblastoma-growth
#4
Mohamed Fareh, Fabien Almairac, Laurent Turchi, Fanny Burel-Vandenbos, Philippe Paquis, Denys Fontaine, Sandra Lacas-Gervais, Marie-Pierre Junier, Hervé Chneiweiss, Thierry Virolle
Glioblastomas are incurable primary brain tumors that affect patients of all ages. The aggressiveness of this cancer has been attributed in part to the persistence of treatment-resistant glioblastoma stem-like cells. We have previously discovered the tumor-suppressor properties of the microRNA cluster miR-302-367, representing a potential treatment for glioblastoma. Here, we attempted to develop a cell-based therapy by taking advantage of the capability of glioma cells to secrete exosomes that enclose small RNA molecules...
March 30, 2017: Cell Death & Disease
https://www.readbyqxmd.com/read/28338200/glioma-cells-promote-angiogenesis-through-the-release-of-exosomes-containing-long-non-coding-rna-pou3f3
#5
H-L Lang, G-W Hu, Y Chen, Y Liu, W Tu, Y-M Lu, L Wu, G-H Xu
OBJECTIVE: Angiogenesis is a key event in the progression of gliomas, and emerging evidence suggests that exosomes are signaling extracellular organelles that modulate the tumor microenvironment and promote angiogenesis and tumor progression. This study aimed to explore the mechanism by which glioma-derived exosomes affect angiogenesis. MATERIALS AND METHODS: qRT-PCR was used to determine the expression level of linc-POU3F3 in glioma tissue as well as glioma cell lines...
March 2017: European Review for Medical and Pharmacological Sciences
https://www.readbyqxmd.com/read/28107450/systemic-t-cells-immunosuppression-of-glioma-stem-cell-derived-exosomes-is-mediated-by-monocytic-myeloid-derived-suppressor-cells
#6
Rossana Domenis, Daniela Cesselli, Barbara Toffoletto, Evgenia Bourkoula, Federica Caponnetto, Ivana Manini, Antonio Paolo Beltrami, Tamara Ius, Miran Skrap, Carla Di Loreto, Giorgia Gri
A major contributing factor to glioma development and progression is its ability to evade the immune system. Nano-meter sized vesicles, exosomes, secreted by glioma-stem cells (GSC) can act as mediators of intercellular communication to promote tumor immune escape. Here, we investigated the immunomodulatory properties of GCS-derived exosomes on different peripheral immune cell populations. Healthy donor peripheral blood mononuclear cells (PBMCs) stimulated with anti-CD3, anti-CD28 and IL-2, were treated with GSC-derived exosomes...
2017: PloS One
https://www.readbyqxmd.com/read/28039693/exosomal-communication-in-glioma-a-review
#7
Hongsheng Xu, Kezhong Zhang, Hailiang Zong, Ming Shang, Kai Li, Xiaoguang He
Cancer research has revealed the existence of cancer stem cells (CSCs). However, the influence of the surrounding stromal cells present in the microenvironment on CSCs is still poorly understood. The latest studies on gliomas suggested that the microenvironment of human gliomas contains both glioma stem cells (GSCs) and glioma associated (GA)-mesenchymal stem cells (MSCs; (GA-MSCs). Also, studies have suggested that nano- sized vesicles, termed exosomes, have been recently observed to contribute towards intercellular communication within the tumor niche...
November 2016: Journal of B.U.ON.: Official Journal of the Balkan Union of Oncology
https://www.readbyqxmd.com/read/27993726/size-dependent-cellular-uptake-of-exosomes
#8
Federica Caponnetto, Ivana Manini, Miran Skrap, Timea Palmai-Pallag, Carla Di Loreto, Antonio Paolo Beltrami, Daniela Cesselli, Enrico Ferrari
The ability of exosomes to elicit specific cellular responses suggests that they may be increasingly used as therapeutics. Their vesicular nature makes them suitable as potential nanocarriers for drugs or nucleic acids delivery. Here we address the question whether the method of preparation of enriched exosomal fractions can affect their uptake by cells and their ability to trigger a response. We compared ultracentrifugation and polymer-based precipitation methods on supernatants of glioma-associated stem cells isolated from a high-grade glioma patient...
April 2017: Nanomedicine: Nanotechnology, Biology, and Medicine
https://www.readbyqxmd.com/read/27902458/dna-sequences-within-glioma-derived-extracellular-vesicles-can-cross-the-intact-blood-brain-barrier-and-be-detected-in-peripheral-blood-of-patients
#9
Noemí García-Romero, Josefa Carrión-Navarro, Susana Esteban-Rubio, Elisa Lázaro-Ibáñez, María Peris-Celda, Marta M Alonso, Juan Guzmán-De-Villoria, Carlos Fernández-Carballal, Ana Ortiz de Mendivil, Sara García-Duque, Carmen Escobedo-Lucea, Ricardo Prat-Acín, Cristóbal Belda-Iniesta, Angel Ayuso-Sacido
Tumor-cell-secreted extracellular vesicles (EVs) can cross the disrupted blood-brain barrier (BBB) into the bloodstream. However, in certain gliomas, the BBB remains intact, which might limit EVs release. To evaluate the ability of tumor-derived EVs to cross the BBB, we used an orthotopic xenotransplant mouse model of human glioma-cancer stem cells featuring an intact BBB. We demonstrated that all types of tumor cells-derived EVs-apoptotic bodies, shedding microvesicles and exosomes-cross the intact BBB and can be detected in the peripheral blood, which provides a minimally invasive method for their detection compared to liquid biopsies obtained from cerebrospinal fluid (CSF)...
January 3, 2017: Oncotarget
https://www.readbyqxmd.com/read/27837435/exosomal-mir-221-targets-dnm3-to-induce-tumor-progression-and-temozolomide-resistance-in-glioma
#10
Jian-Kai Yang, Ji-Peng Yang, Jing Tong, Shi-Yuan Jing, Bo Fan, Feng Wang, Guo-Zhu Sun, Bao-Hua Jiao
MicroRNA is an important regulator of glioblastoma. This study aims at validating microRNA-221 (miR-221) as a biomarker for glioblastoma, and understanding how miR-221 regulates glioblastoma progression. Using clinical samples, miR-221 expression was analyzed by quantitative reverse-transcriptase PCR (qPCR). SHG-44 cells were treated with anti-miR-221 or U87MG-derived exosomes followed by monitoring changes in cell viability, migration and temozolomide (TMZ) resistance. Bioinformatics approach was used to identify targets of miR-221...
November 11, 2016: Journal of Neuro-oncology
https://www.readbyqxmd.com/read/27771233/the-emergent-role-of-exosomes-in-glioma
#11
REVIEW
J Gourlay, A P Morokoff, R B Luwor, H-J Zhu, A H Kaye, S S Stylli
Extracellular vesicles (EVs) are known mediators of intercellular communication for both normal and tumour cells. With the capability to transfer nucleic acids, proteins and lipids, EVs are able to influence numerous functional and pathological aspects of both donor and recipient cells. The tumour microenvironment possesses a high level of complex heterogeneity, particularly within the most prominent brain malignancy, glioblastoma multiforme (GBM). This complexity relies on a network-based communication between many different components of the local niche, including the various cell types, stroma, blood vessels, secreted factors and surrounding matrix...
January 2017: Journal of Clinical Neuroscience: Official Journal of the Neurosurgical Society of Australasia
https://www.readbyqxmd.com/read/26983831/exosomes-as-tools-to-suppress-primary-brain-tumor
#12
REVIEW
Mark Katakowski, Michael Chopp
Exosomes are small microvesicles released by cells that efficiently transfer their molecular cargo to other cells, including tumor. Exosomes may pass the blood-brain barrier and have been demonstrated to deliver RNAs contained within to brain. As they are non-viable, the risk profile of exosomes is thought to be less than that of cellular therapies. Exosomes can be manufactured at scale in culture, and exosomes can be engineered to incorporate therapeutic miRNAs, siRNAs, or chemotherapeutic molecules. As natural biological delivery vehicles, interest in the use of exosomes as therapeutic delivery agents is growing...
April 2016: Cellular and Molecular Neurobiology
https://www.readbyqxmd.com/read/26711578/the-limited-capacity-of-malignant-glioma-derived-exosomes-to-suppress-peripheral-immune-effectors
#13
J Bryan Iorgulescu, Michael E Ivan, Michael Safaee, Andrew T Parsa
Tumor-derived microvesicular exosomes permit intercellular communication both locally and systemically by delivering a snapshot of the tumor cell's constituents. We thus investigated whether exosomes mediate malignant glioma's facility for inducing peripheral immunosuppression. In Western blot and RT-PCR analyses, glioma-derived exosomes displayed exosome-specific markers, but failed to recapitulate the antigen-presentation machinery, surface co-modulatory signals, or immunosuppressive mediator status of their parent tumor cells...
January 15, 2016: Journal of Neuroimmunology
https://www.readbyqxmd.com/read/26620801/phosphorylation-negatively-regulates-exosome-mediated-secretion-of-cryab-in-glioma-cells
#14
Rajshekhar A Kore, Edathara C Abraham
Exosomes mediate secretion of crystallin alphaB (cryAB), a well characterized molecular chaperone with anti-apoptotic activity. However, the mechanisms governing its packaging and secretion remained unexplored. In glioma cells, notwithstanding extensive phosphorylation of cryAB at Ser59 followed by Ser45 (Ser19 is largely unphosphorylated), we discovered that the majority of secreted exosomal cryAB is nonphosphorylated. Transient ectopic expression of a yellow fluorescent protein (YFP) tagged triple phosphomimic (3-SD) cryAB construct in cryAB absent glioma cells led to the formation of large cytosolic inclusions...
February 2016: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/26284486/exosomal-levels-of-mirna-21-from-cerebrospinal-fluids-associated-with-poor-prognosis-and-tumor-recurrence-of-glioma-patients
#15
Rui Shi, Pei-Yin Wang, Xin-Yi Li, Jian-Xin Chen, Yan Li, Xin-Zhong Zhang, Chen-Guang Zhang, Tao Jiang, Wen-Bin Li, Wei Ding, Shu-Jun Cheng
Glioma is a most common type of primary brain tumors. Extracellular vesicles, in the form of exosomes, are known to mediate cell-cell communication by transporting cell-derived proteins and nucleic acids, including various microRNAs (miRNAs). Here we examined the cerebrospinal fluid (CSF) from patients with recurrent glioma for the levels of cancer-related miRNAs, and evaluated the values for prognosis by comparing the measures of CSF-, serum-, and exosome-contained miR-21 levels. Samples from seventy glioma patients following surgery were compared with those from brain trauma patients as a non-tumor control group...
September 29, 2015: Oncotarget
https://www.readbyqxmd.com/read/26172304/exposure-to-als-ftd-csf-generates-tdp-43-aggregates-in-glioblastoma-cells-through-exosomes-and-tnts-like-structure
#16
Xuebing Ding, Mingming Ma, Junfang Teng, Robert K F Teng, Shuang Zhou, Jingzheng Yin, Ekokobe Fonkem, Jason H Huang, Erxi Wu, Xuejing Wang
Amyotrophic lateral sclerosis (ALS) and frontotemporal dementia (FTD) represent a continuum of devastating neurodegenerative diseases, characterized by transactive response DNA-binding protein of 43 kDa (TDP-43) aggregates accumulation throughout the nervous system. Despite rapidly emerging evidence suggesting the hypothesis of 'prion-like propagation' of TDP-43 positive inclusion in the regional spread of ALS symptoms, whether and how TDP-43 aggregates spread between cells is not clear. Herein, we established a cerebrospinal fluid (CSF)-cultured cell model to dissect mechanisms governing TDP-43 aggregates formation and propagation...
September 15, 2015: Oncotarget
https://www.readbyqxmd.com/read/26155418/extracellular-vesicle-mediated-transfer-of-functional-rna-in-the-tumor-microenvironment
#17
Kirsten Ridder, Alexandra Sevko, Janina Heide, Maria Dams, Anne-Kathleen Rupp, Jadranka Macas, Julia Starmann, Marc Tjwa, Karl H Plate, Holger Sültmann, Peter Altevogt, Viktor Umansky, Stefan Momma
Extracellular vesicles (EVs) have been shown to transfer various molecules, including functional RNA between cells and this process has been suggested to be particularly relevant in tumor-host interactions. However, data on EV-mediated RNA transfer has been obtained primarily by in vitro experiments or involving ex vivo manipulations likely affecting its biology, leaving their physiological relevance unclear. We engineered glioma and carcinoma tumor cells to express Cre recombinase showing their release of EVs containing Cre mRNA in various EV subfractions including exosomes...
June 2015: Oncoimmunology
https://www.readbyqxmd.com/read/26155415/exosomes-isolated-from-plasma-of-glioma-patients-enrolled-in-a-vaccination-trial-reflect-antitumor-immune-activity-and-might-predict-survival
#18
Laurent Muller, Sylvia Muller-Haegele, Masato Mitsuhashi, William Gooding, Hideho Okada, Theresa L Whiteside
Exosomes in plasma of glioma patients hold promise as biomarkers of prognosis. We aimed to determine whether changes in total exosomal protein and mRNA expression levels could serve as surrogate markers of immunological and clinical responses in glioma patients receiving antitumor vaccines. Exosomes were isolated from pre/post-vaccine plasma specimens in 20/22 patients enrolled in a phase I/II trial with the antitumor vaccine. Exosomal protein content was analyzed and mRNA expression levels for 24 genes were simultaneously assessed by qRT-PCR...
June 2015: Oncoimmunology
https://www.readbyqxmd.com/read/25721810/extracellular-vesicles-in-the-biology-of-brain-tumour-stem-cells-implications-for-inter-cellular-communication-therapy-and-biomarker-development
#19
REVIEW
Ichiro Nakano, Delphine Garnier, Mutsuko Minata, Janusz Rak
Extracellular vesicles (EVs) act as carriers of molecular and oncogenic signatures present in subsets of tumour cells and tumour-associated stroma, and as mediators of intercellular communication. These processes likely involve cancer stem cells (CSCs). EVs represent a unique pathway of cellular export and cell-to-cell transfer of insoluble molecular regulators such as membrane receptors, signalling proteins and metabolites, thereby influencing the functional integration of cancer cell populations. While mechanisms that control biogenesis, cargo and uptake of different classes of EVs (exosomes, microvesicles, ectosomes, large oncosomes) are poorly understood, they likely remain under the influence of stress-responses, microenvironment and oncogenic processes that define the biology and heterogeneity of human cancers...
April 2015: Seminars in Cell & Developmental Biology
https://www.readbyqxmd.com/read/25680514/exosomes-from-dendritic-cells-loaded-with-chaperone-rich-cell-lysates-elicit-a-potent-t-cell-immune-response-against-intracranial-glioma-in-mice
#20
Ning Bu, Haiqin Wu, Guilian Zhang, Shuqin Zhan, Ru Zhang, Hong Sun, Yun Du, Li Yao, Huqing Wang
Chaperone-rich cell lysates (CRCLs) may play an important role in the development of anti-tumor vaccines. Tumor-derived CRCLs have been reported to activate dendritic cells (DCs) to elicit potent anti-tumor activity. However, the role of DC-derived exosomes (DEXs) secreted from DCs loaded with CRCLs in the treatment of tumors has not been clearly determined. In the present study, DEXs were generated from DCs loaded with CRCLs derived from GL261 glioma cells. These DEXs, designated DEX (CRCL-GL261), were then used to treat DCs to create DEX (CRCL-GL261)-DCs...
July 2015: Journal of Molecular Neuroscience: MN
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