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Glioma AND exosome

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https://www.readbyqxmd.com/read/29076027/serum-exosomal-mir-301a-as-a-potential-diagnostic-and-prognostic-biomarker-for-human-glioma
#1
Fengming Lan, Qin Qing, Qiang Pan, Man Hu, Huiming Yu, Xiao Yue
PURPOSE: Exosomal miRNAs that play an important role in cell-cell communication have attracted major attention as potential diagnostic and prognostic biomarkers for various cancers. The aim of this study was to determine the diagnostic/prognostic significance of serum exosomal miR-301a in glioma patients. METHODS: Quantitative real-time PCR was used to determine the serum exosomal expression levels of miR-301a. Kaplan-Meier survival analyses, Cox regression analyses and ROC working curve analyses were applied to assess the diagnostic and prognostic values of miR-301a in glioma patients...
October 26, 2017: Cellular Oncology (Dordrecht)
https://www.readbyqxmd.com/read/29076001/a-microrna-signature-from-serum-exosomes-of-patients-with-glioma-as-complementary-diagnostic-biomarker
#2
Alessandra Santangelo, Pietro Imbrucè, Beatrice Gardenghi, Laura Belli, Rina Agushi, Anna Tamanini, Silvia Munari, Alessandra Maria Bossi, Ilaria Scambi, Donatella Benati, Raffaella Mariotti, Gianfranco Di Gennaro, Andrea Sbarbati, Albino Eccher, Giuseppe Kenneth Ricciardi, Elisa Maria Ciceri, Francesco Sala, Giampietro Pinna, Giuseppe Lippi, Giulio Cabrini, Maria Cristina Dechecchi
Malignant gliomas, the most frequent primary brain tumors, are characterized by a dismal prognosis. Reliable biomarkers complementary to neuroradiology in the differential diagnosis of gliomas and monitoring for post-surgical progression are unmet needs. Altered expression of several microRNAs in tumour tissues from patients with gliomas compared to normal brain tissue have been described, thus supporting the rationale of using microRNA-based biomarkers. Although different circulating microRNAs were proposed in association with gliomas, they have not been introduced into clinical practice so far...
October 26, 2017: Journal of Neuro-oncology
https://www.readbyqxmd.com/read/29074968/coding-and-noncoding-landscape-of-extracellular-rna-released-by-human-glioma-stem-cells
#3
Zhiyun Wei, Arsen O Batagov, Sergio Schinelli, Jintu Wang, Yang Wang, Rachid El Fatimy, Rosalia Rabinovsky, Leonora Balaj, Clark C Chen, Fred Hochberg, Bob Carter, Xandra O Breakefield, Anna M Krichevsky
Tumor-released RNA may mediate intercellular communication and serve as biomarkers. Here we develop a protocol enabling quantitative, minimally biased analysis of extracellular RNAs (exRNAs) associated with microvesicles, exosomes (collectively called EVs), and ribonucleoproteins (RNPs). The exRNA complexes isolated from patient-derived glioma stem-like cultures exhibit distinct compositions, with microvesicles most closely reflecting cellular transcriptome. exRNA is enriched in small ncRNAs, such as miRNAs in exosomes, and precisely processed tRNA and Y RNA fragments in EVs and exRNPs...
October 26, 2017: Nature Communications
https://www.readbyqxmd.com/read/29050084/-recent-progress-in-application-of-exosome-in-diagnosis-and-treatment-of-gliomas
#4
W W Xu, L M Wang, L H Teng
No abstract text is available yet for this article.
October 8, 2017: Zhonghua Bing Li Xue za Zhi Chinese Journal of Pathology
https://www.readbyqxmd.com/read/29016925/divergent-evolution-of-temozolomide-resistance-in-glioblastoma-stem-cells-is-reflected-in-extracellular-vesicles-and-coupled-with-radiosensitization
#5
Delphine Garnier, Brian Meehan, Thomas Kislinger, Paul Daniel, Ankit Sinha, Bassam Abdulkarim, Ichiro Nakano, Janusz Rak
Background: Glioblastoma (GBM) is almost invariably fatal due to failure of standard therapy. The relapse of GBM following surgery, radiation and systemic temozolomide (TMZ) is attributed to the ability of glioma stem cells (GSCs) to survive, evolve and repopulate the tumor mass, events on which therapy exerts a poorly understood influence. Methods: Here we explore the molecular and cellular evolution of TMZ resistance as it emerges in vivo (xenograft models) in a series of human GSCs with either proneural (PN) or mesenchymal (MES) molecular characteristics...
July 28, 2017: Neuro-oncology
https://www.readbyqxmd.com/read/29016843/mesenchymal-stem-cells-as-natural-bio-factories-for-exosomes-carrying-mir-124a-in-the-treatment-of-gliomas
#6
Frederick M Lang, Anwar Hossain, Joy Gumin, Eric N Momin, Yuzaburo Shimizu, Dan Ledbetter, Tal Shahar, Shinji Yamashita, Brittany Parker-Kerrigan, Juan Fueyo, Raymond Sawaya, Frederick F Lang
Background: MicroRNAs (miRs) are promising new therapeutics for glioblastoma. However, which miRs are most effective against glioblastomas and how these miRs should be delivered are major unanswered problems. Methods: To identify potent anti-glioma miRs, we selected eight miRs based on a literature search and screened them against a panel of glioma stem cell lines (GSCs), representing all TCGA-defined glioblastoma subtypes. To address delivery, we tested the hypothesis that ex vivo-cultured bone marrow-derived mesenchymal stem cells (MSCs) can package miRs into exosomes and that these engineered exosomes can systemically deliver antiglioma miRs to glioblastomas...
August 14, 2017: Neuro-oncology
https://www.readbyqxmd.com/read/28948499/cd133-positive-u87-glioblastoma-cells-derived-exosomal-micrornas-in-hypoxia-versus-normoxia-microenviroment
#7
Guobin Zhang, Yunsheng Zhang, Sen Cheng, Zhen Wu, Fusheng Liu, Junting Zhang
Hypoxia is a major regulator of glioma development and aggressiveness. However, how CD133 positive U87 glioblastoma cells adapt to hypoxia and communicate with their surrounding microenvironment during tumor development remain important questions. Communication with host cells and stroma via exosomes represents one pathway by which tumors can modify their surroundings to achieve a tumor-permissive environment. MicroRNAs are thought to be essential actors of tumorigenesis as they are able to control the expression of numerous genes...
October 2017: Journal of Neuro-oncology
https://www.readbyqxmd.com/read/28855213/exosomes-from-glioma-associated-mesenchymal-stem-cells-increase-the-tumorigenicity-of-glioma-stem-like-cells-via-transfer-of-mir-1587
#8
Javier Figueroa, Lynette M Phillips, Tal Shahar, Anwar Hossain, Joy Gumin, Hoon Kim, Andrew J Bean, George A Calin, Juan Fueyo, Edgar T Walters, Raghu Kalluri, Roel G Verhaak, Frederick F Lang
Tumor-stromal communications impact tumorigenesis in ways that are incompletely understood. Here, we show that glioma-associated human mesenchymal stem cells (GA-hMSC), a newly identified stromal component of glioblastoma, release exosomes that increase the proliferation and clonogenicity of tumor-initiating glioma stem-like cells (GSC). This event leads to a significantly greater tumor burden and decreased host survival compared with untreated GSCs in orthotopic xenografts. Analysis of the exosomal content identified miR-1587 as a mediator of the exosomal effects on GSCs, in part via downregulation of the tumor-suppressive nuclear receptor corepressor NCOR1...
November 1, 2017: Cancer Research
https://www.readbyqxmd.com/read/28844885/glioma-progression-through-the-prism-of-heat-shock-protein-mediated-extracellular-matrix-remodeling-and-epithelial-to-mesenchymal-transition
#9
REVIEW
Y Rajesh, Angana Biswas, Mahitosh Mandal
Glial tumor is one of the intrinsic brain tumors with high migratory and infiltrative potential. This essentially contributes to the overall poor prognosis by circumvention of conventional treatment regimen in glioma. The underlying mechanism in gliomagenesis is bestowed by two processes- Extracellular matrix (ECM) Remodeling and Epithelial to mesenchymal transition (EMT). Heat Shock Family of proteins (HSPs), commonly known as "molecular chaperons" are documented to be upregulated in glioma. A positive correlation also exists between elevated expression of HSPs and invasive capacity of glial tumor...
October 15, 2017: Experimental Cell Research
https://www.readbyqxmd.com/read/28687495/extracellular-vesicles-novel-promising-delivery-systems-for-therapy-of-brain-diseases
#10
REVIEW
David Rufino-Ramos, Patrícia R Albuquerque, Vitor Carmona, Rita Perfeito, Rui Jorge Nobre, Luis Pereira de Almeida
Extracellular vesicles (EVs) are cell-derived membrane vesicles virtually secreted by all cells, including brain cells. EVs are a major term that includes apoptotic bodies, microvesicles and exosomes. The release of EVs has been recognized as an important modulator in cross-talking between neurons, astrocytes, microglia and oligodendrocytes, not only in central nervous system (CNS) physiology but also in neurodegenerative and neuroinflammatory disease states as well as in brain tumors, such as glioma. EVs are able to cross the blood brain barrier (BBB), spread to body fluids and reach distant tissues...
July 4, 2017: Journal of Controlled Release: Official Journal of the Controlled Release Society
https://www.readbyqxmd.com/read/28656228/glioma-cells-enhance-angiogenesis-and-inhibit-endothelial-cell-apoptosis-through-the-release-of-exosomes-that-contain-long-non-coding-rna-ccat2
#11
Hai-Li Lang, Guo-Wen Hu, Bo Zhang, Wei Kuang, Yong Chen, Lei Wu, Guo-Hai Xu
Angiogenesis is a key event in the progression of gliomas. Exosomes, as signaling extracellular organelles, modulate the tumor microenvironment and promote angiogenesis and tumor progression. We previously demonstrated that long intergenic non-coding RNA CCAT2 (linc-CCAT2) was overexpressed in glioma tissues and functioned to promote glioma progression. Therefore, this study aimed to explore an underlying mechanism of glioma cell-affected angiogenesis. First, qRT-PCR was used to determine the expression level of linc-CCAT2 in 4 glioma cell lines and 293T cells, and the results revealed that the U87-MG cells exhibited the highest expression level...
August 2017: Oncology Reports
https://www.readbyqxmd.com/read/28583903/interleukin-13-conjugated-quantum-dots-for-identification-of-glioma-initiating-cells-and-their-extracellular-vesicles
#12
A B Madhankumar, Oliver D Mrowczynski, Suhag R Patel, Cody L Weston, Brad E Zacharia, Michael J Glantz, Christopher A Siedlecki, Li-Chong Xu, James R Connor
Cadmium selenide (CdSe) based quantum dots modified with polyethylene glycol and chemically linked to interleukin-13 (IL13) were prepared with the aim of identifying the high affinity receptor (IL13Rα2) which is expressed in glioma stem cells and exosomes secreted by these cancer stem cells. IL13 conjugated quantum dots (IL13QD) were thoroughly characterized for their physicochemical properties including particle size and surface morphology. Furthermore, the specific binding of the IL13QD to glioma cells and to glioma stem cells (GSC) was verified using a competitive binding study...
June 3, 2017: Acta Biomaterialia
https://www.readbyqxmd.com/read/28574310/exosomes-as-a-biomarker-platform-for-detecting-epidermal-growth-factor-receptor-positive-high-grade-gliomas
#13
Sasidhar Venkata Manda, Yogesh Kataria, Babul Reddy Tatireddy, Balasubramaniam Ramakrishnan, Boola Gnana Ratnam, Rahul Lath, Alok Ranjan, Amitava Ray
OBJECTIVE High-grade glial brain tumors are often characterized by an elevated expression of the tumorigenic epidermal growth factor receptor variant III ( EGFRvIII). The authors sought to establish a clinically adaptive protocol as a noninvasive diagnostic tool for EGFRvIII detection through serum exosomes. METHODS Purity of serum exosome/RNA was confirmed by electron microscopy and flow cytometry and through an RNA bioanalyzer profile. EGFRvIII amplification was initially established by semiquantitative polymerase chain reaction in tumor tissues and exosomes...
June 2, 2017: Journal of Neurosurgery
https://www.readbyqxmd.com/read/28410224/glioma-stem-cells-derived-exosomes-promote-the-angiogenic-ability-of-endothelial-cells-through-mir-21-vegf-signal
#14
Xu Sun, Xiaotang Ma, Jinju Wang, Yuhui Zhao, Yue Wang, Ji C Bihl, Yanfang Chen, Chuanlu Jiang
Glioma stem cells (GSCs) play an important role in glioblastoma prognosis. Exosomes (EXs) mediate cell communication by delivering microRNAs (miRs). Glioblastoma has a high level of miR-21 which could upregulate vascular endothelial growth factor (VEGF) expression. We hypothesized GSC-EXs can promote the angiogenic ability of endothelial cells (ECs) through miR-21/VEGF signal. GSCs were isolated from U-251 cells with stem cell marker CD133. GSCs transfected without or with scramble or miR-21 mimics were used to produce GSC-EXscon, GSC-EXssc and GSC-EXsmiR-21...
May 30, 2017: Oncotarget
https://www.readbyqxmd.com/read/28358371/cell-based-therapy-using-mir-302-367-expressing-cells-represses-glioblastoma-growth
#15
Mohamed Fareh, Fabien Almairac, Laurent Turchi, Fanny Burel-Vandenbos, Philippe Paquis, Denys Fontaine, Sandra Lacas-Gervais, Marie-Pierre Junier, Hervé Chneiweiss, Thierry Virolle
Glioblastomas are incurable primary brain tumors that affect patients of all ages. The aggressiveness of this cancer has been attributed in part to the persistence of treatment-resistant glioblastoma stem-like cells. We have previously discovered the tumor-suppressor properties of the microRNA cluster miR-302-367, representing a potential treatment for glioblastoma. Here, we attempted to develop a cell-based therapy by taking advantage of the capability of glioma cells to secrete exosomes that enclose small RNA molecules...
March 30, 2017: Cell Death & Disease
https://www.readbyqxmd.com/read/28338200/glioma-cells-promote-angiogenesis-through-the-release-of-exosomes-containing-long-non-coding-rna-pou3f3
#16
H-L Lang, G-W Hu, Y Chen, Y Liu, W Tu, Y-M Lu, L Wu, G-H Xu
OBJECTIVE: Angiogenesis is a key event in the progression of gliomas, and emerging evidence suggests that exosomes are signaling extracellular organelles that modulate the tumor microenvironment and promote angiogenesis and tumor progression. This study aimed to explore the mechanism by which glioma-derived exosomes affect angiogenesis. MATERIALS AND METHODS: qRT-PCR was used to determine the expression level of linc-POU3F3 in glioma tissue as well as glioma cell lines...
March 2017: European Review for Medical and Pharmacological Sciences
https://www.readbyqxmd.com/read/28107450/systemic-t-cells-immunosuppression-of-glioma-stem-cell-derived-exosomes-is-mediated-by-monocytic-myeloid-derived-suppressor-cells
#17
Rossana Domenis, Daniela Cesselli, Barbara Toffoletto, Evgenia Bourkoula, Federica Caponnetto, Ivana Manini, Antonio Paolo Beltrami, Tamara Ius, Miran Skrap, Carla Di Loreto, Giorgia Gri
A major contributing factor to glioma development and progression is its ability to evade the immune system. Nano-meter sized vesicles, exosomes, secreted by glioma-stem cells (GSC) can act as mediators of intercellular communication to promote tumor immune escape. Here, we investigated the immunomodulatory properties of GCS-derived exosomes on different peripheral immune cell populations. Healthy donor peripheral blood mononuclear cells (PBMCs) stimulated with anti-CD3, anti-CD28 and IL-2, were treated with GSC-derived exosomes...
2017: PloS One
https://www.readbyqxmd.com/read/28039693/exosomal-communication-in-glioma-a-review
#18
Hongsheng Xu, Kezhong Zhang, Hailiang Zong, Ming Shang, Kai Li, Xiaoguang He
Cancer research has revealed the existence of cancer stem cells (CSCs). However, the influence of the surrounding stromal cells present in the microenvironment on CSCs is still poorly understood. The latest studies on gliomas suggested that the microenvironment of human gliomas contains both glioma stem cells (GSCs) and glioma associated (GA)-mesenchymal stem cells (MSCs; (GA-MSCs). Also, studies have suggested that nano- sized vesicles, termed exosomes, have been recently observed to contribute towards intercellular communication within the tumor niche...
November 2016: Journal of B.U.ON.: Official Journal of the Balkan Union of Oncology
https://www.readbyqxmd.com/read/27993726/size-dependent-cellular-uptake-of-exosomes
#19
Federica Caponnetto, Ivana Manini, Miran Skrap, Timea Palmai-Pallag, Carla Di Loreto, Antonio Paolo Beltrami, Daniela Cesselli, Enrico Ferrari
The ability of exosomes to elicit specific cellular responses suggests that they may be increasingly used as therapeutics. Their vesicular nature makes them suitable as potential nanocarriers for drugs or nucleic acids delivery. Here we address the question whether the method of preparation of enriched exosomal fractions can affect their uptake by cells and their ability to trigger a response. We compared ultracentrifugation and polymer-based precipitation methods on supernatants of glioma-associated stem cells isolated from a high-grade glioma patient...
April 2017: Nanomedicine: Nanotechnology, Biology, and Medicine
https://www.readbyqxmd.com/read/27902458/dna-sequences-within-glioma-derived-extracellular-vesicles-can-cross-the-intact-blood-brain-barrier-and-be-detected-in-peripheral-blood-of-patients
#20
Noemí García-Romero, Josefa Carrión-Navarro, Susana Esteban-Rubio, Elisa Lázaro-Ibáñez, María Peris-Celda, Marta M Alonso, Juan Guzmán-De-Villoria, Carlos Fernández-Carballal, Ana Ortiz de Mendivil, Sara García-Duque, Carmen Escobedo-Lucea, Ricardo Prat-Acín, Cristóbal Belda-Iniesta, Angel Ayuso-Sacido
Tumor-cell-secreted extracellular vesicles (EVs) can cross the disrupted blood-brain barrier (BBB) into the bloodstream. However, in certain gliomas, the BBB remains intact, which might limit EVs release. To evaluate the ability of tumor-derived EVs to cross the BBB, we used an orthotopic xenotransplant mouse model of human glioma-cancer stem cells featuring an intact BBB. We demonstrated that all types of tumor cells-derived EVs-apoptotic bodies, shedding microvesicles and exosomes-cross the intact BBB and can be detected in the peripheral blood, which provides a minimally invasive method for their detection compared to liquid biopsies obtained from cerebrospinal fluid (CSF)...
January 3, 2017: Oncotarget
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