keyword
MENU ▼
Read by QxMD icon Read
search

Oxygen sensitive sequence

keyword
https://www.readbyqxmd.com/read/27907091/real-time-fluorescence-measurements-of-ros-and-ca2-in-ischemic-reperfused-rat-hearts-detectable-increases-occur-only-after-mitochondrial-pore-opening-and-are-attenuated-by-ischemic-preconditioning
#1
Tatyana Andrienko, Philippe Pasdois, Andreas Rossbach, Andrew P Halestrap
Mitochondrial permeability transition pore (mPTP) opening is critical for ischemia / reperfusion (I/R) injury and is associated with increased [Ca2+] and reactive oxygen species (ROS). Here we employ surface fluorescence to establish the temporal sequence of these events in beating perfused hearts subject to global I/R. A bespoke fluorimeter was used to synchronously monitor surface fluorescence and reflectance of Langendorff-perfused rat hearts at multiple wavelengths, with simultaneous measurements of hemodynamic function...
2016: PloS One
https://www.readbyqxmd.com/read/27886462/loss-of-ppr3-ppr4-ppr6-or-ppr10-perturbs-iron-homeostasis-and-leads-to-apoptotic-cell-death-in-schizosaccharomyces-pombe
#2
Yang Su, Yanmei Yang, Ying Huang
Pentatricopeptide repeat (PPR) proteins characterized by tandem arrays of a degenerate 35-amino-acid repeat belong to a large family of RNA-binding proteins that are involved in posttranscriptional control of organelle gene expression. PPR proteins are ubiquitous in eukaryotes, and particularly prevalent in higher plants. Schizosaccharomyces pombe has ten PPR proteins. Among them, ppr3, ppr4, ppr6 and ppr10 participate in mitochondrial posttranscriptional processes and are required for mitochondrial electron transport chain (ETC) function...
November 25, 2016: FEBS Journal
https://www.readbyqxmd.com/read/27883213/oxidative-stress-and-amyloid-toxicity-insights-from-yeast
#3
Mauro B França, Karina C Lima, Elis C A Eleutherio
Alzheimer's disease is the most common neurodegenerative disorder. One of the factors that promotes neurodegeneration is the accumulation of senile plaques formed by Aβ peptide. In this paper it was analyzed if oxidative stress is cause or consequence of amyloid cascade and the role of antioxidant defense system in this process, using S. cerevisiae (with a multicopy plasmid containing the Aβ1-42 sequence) as experimental model. Cells grown on glycerol were more tolerant than when grown on glucose, strengthening the role of the antioxidant defense system against Aβ accumulation...
November 24, 2016: Journal of Cellular Biochemistry
https://www.readbyqxmd.com/read/27863297/degenerate-cysteine-patterns-mediate-two-redox-sensing-mechanisms-in-the-papillomavirus-e7-oncoprotein
#4
Gabriela Camporeale, Juan R Lorenzo, Maria G Thomas, Edgardo Salvatierra, Silvia S Borkosky, Marikena G Risso, Ignacio E Sánchez, Gonzalo de Prat Gay, Leonardo G Alonso
Infection with oncogenic human papillomavirus induces deregulation of cellular redox homeostasis. Virus replication and papillomavirus-induced cell transformation require persistent expression of viral oncoproteins E7 and E6 that must retain their functionality in a persistent oxidative environment. Here, we dissected the molecular mechanisms by which E7 oncoprotein can sense and manage the potentially harmful oxidative environment of the papillomavirus-infected cell. The carboxy terminal domain of E7 protein from most of the 79 papillomavirus viral types of alpha genus, which encloses all the tumorigenic viral types, is a cysteine rich domain that contains two classes of cysteines: strictly conserved low reactive Zn(+2) binding and degenerate reactive cysteine residues that can sense reactive oxygen species (ROS)...
November 12, 2016: Redox Biology
https://www.readbyqxmd.com/read/27854210/skeletal-muscle-quantitative-nuclear-magnetic-resonance-imaging-and-spectroscopy-as-an-outcome-measure-for-clinical-trials
#5
Pierre G Carlier, Benjamin Marty, Olivier Scheidegger, Paulo Loureiro de Sousa, Pierre-Yves Baudin, Eduard Snezhko, Dmitry Vlodavets
Recent years have seen tremendous progress towards therapy of many previously incurable neuromuscular diseases. This new context has acted as a driving force for the development of novel non-invasive outcome measures. These can be organized in three main categories: functional tools, fluid biomarkers and imagery. In the latest category, nuclear magnetic resonance imaging (NMRI) offers a considerable range of possibilities for the characterization of skeletal muscle composition, function and metabolism. Nowadays, three NMR outcome measures are frequently integrated in clinical research protocols...
March 3, 2016: Journal of Neuromuscular Diseases
https://www.readbyqxmd.com/read/27812217/activation-of-bicyclic-nitro-drugs-by-a-novel-nitroreductase-ntr2-in-leishmania
#6
Susan Wyllie, Adam J Roberts, Suzanne Norval, Stephen Patterson, Bernardo J Foth, Matthew Berriman, Kevin D Read, Alan H Fairlamb
Drug discovery pipelines for the "neglected diseases" are now heavily populated with nitroheterocyclic compounds. Recently, the bicyclic nitro-compounds (R)-PA-824, DNDI-VL-2098 and delamanid have been identified as potential candidates for the treatment of visceral leishmaniasis. Using a combination of quantitative proteomics and whole genome sequencing of susceptible and drug-resistant parasites we identified a putative NAD(P)H oxidase as the activating nitroreductase (NTR2). Whole genome sequencing revealed that deletion of a single cytosine in the gene for NTR2 that is likely to result in the expression of a non-functional truncated protein...
November 2016: PLoS Pathogens
https://www.readbyqxmd.com/read/27799778/the-potential-role-for-use-of-mitochondrial-dna-copy-number-as-predictive-biomarker-in-presbycusis
#7
Masoumeh Falah, Massoud Houshmand, Mohammad Najafi, Maryam Balali, Saeid Mahmoudian, Alimohamad Asghari, Hessamaldin Emamdjomeh, Mohammad Farhadi
OBJECTIVES: Age-related hearing impairment, or presbycusis, is the most common communication disorder and neurodegenerative disease in the elderly. Its prevalence is expected to increase, due to the trend of growth of the elderly population. The current diagnostic test for detection of presbycusis is implemented after there has been a change in hearing sensitivity. Identification of a pre-diagnostic biomarker would raise the possibility of preserving hearing sensitivity before damage occurs...
2016: Therapeutics and Clinical Risk Management
https://www.readbyqxmd.com/read/27793828/discordant-temporal-turnovers-of-sediment-bacterial-and-eukaryotic-communities-in-response-to-dredging-non-resilience-and-functional-changes
#8
Na Zhang, Xian Xiao, Meng Pei, Xiang Liu, Yuting Liang
: To study the stability and succession of sediment microbial and macrobenthic communities in response to anthropogenic disturbance, a time-series sampling was conducted before, during and one year after dredging in the Guan River in Changzhou, China, which was performed with cutter suction dredgers from April 10 to May 20, 2014. The microbial communities were analyzed by sequencing bacterial 16S rRNA and eukaryotic 18S rRNA gene amplicons with Illumina MiSeq and the macrobenthic community was identified using a morphological approach simultaneously...
October 28, 2016: Applied and Environmental Microbiology
https://www.readbyqxmd.com/read/27790189/photosynthetic-versatility-in-the-genome-of-geitlerinema-sp-pcc-9228-formerly-oscillatoria-limnetica-solar-lake-a-model-anoxygenic-photosynthetic-cyanobacterium
#9
Sharon L Grim, Gregory J Dick
Anoxygenic cyanobacteria that use sulfide as the electron donor for photosynthesis are a potentially influential but poorly constrained force on Earth's biogeochemistry. Their versatile metabolism may have boosted primary production and nitrogen cycling in euxinic coastal margins in the Proterozoic. In addition, they represent a biological mechanism for limiting the accumulation of atmospheric oxygen, especially before the Great Oxidation Event and in the low-oxygen conditions of the Proterozoic. In this study, we describe the draft genome sequence of Geitlerinema sp...
2016: Frontiers in Microbiology
https://www.readbyqxmd.com/read/27775841/evaluation-of-an-fmri-uspio-based-assay-in-healthy-human-volunteers
#10
Richard Baumgartner, William Cho, Alexandre Coimbra, Christopher Chen, Zaiqi Wang, Arie Struyk, Narayanaswamy Venketasubramanian, May Low, Cindy Gargano, Fuqiang Zhao, Donald Williams, Torsten Reese, Stephanie Seah, Dai Feng, Sonya Apreleva, Esben Petersen, Jeffrey L Evelhoch
PURPOSE: To present the testretest and contrast dose effect results of cerebral blood volume (CBV) functional MRI (fMRI) in healthy human volunteers using ferumoxytol (Feraheme), an ultrasmall-superparamagnetic iron oxide (USPIO) nanoparticle. MATERIALS AND METHODS: This was an open-label, two-period, fixed-sequence study in healthy young volunteers. In eight subjects, using a 3 Tesla field strength system, blood oxygen level dependent (BOLD) and CBV fMRI were acquired in response to a visual black-and-white checkboard stimulation paradigm using an escalating ferumoxytol dose design (250, 350, and 510 mg iron)...
October 24, 2016: Journal of Magnetic Resonance Imaging: JMRI
https://www.readbyqxmd.com/read/27769568/specific-frontostriatal-circuits-for-impaired-cognitive-flexibility-and-goal-directed-planning-in-obsessive-compulsive-disorder-evidence-from-resting-state-functional-connectivity
#11
Matilde M Vaghi, Petra E Vértes, Manfred G Kitzbichler, Annemieke M Apergis-Schoute, Febe E van der Flier, Naomi A Fineberg, Akeem Sule, Rashid Zaman, Valerie Voon, Prantik Kundu, Edward T Bullmore, Trevor W Robbins
BACKGROUND: A recent hypothesis has suggested that core deficits in goal-directed behavior in obsessive-compulsive disorder (OCD) are caused by impaired frontostriatal function. We tested this hypothesis in OCD patients and control subjects by relating measures of goal-directed planning and cognitive flexibility to underlying resting-state functional connectivity. METHODS: Multiecho resting-state acquisition, combined with micromovement correction by blood oxygen level-dependent sensitive independent component analysis, was used to obtain in vivo measures of functional connectivity in 44 OCD patients and 43 healthy comparison subjects...
August 11, 2016: Biological Psychiatry
https://www.readbyqxmd.com/read/27743509/remote-vital-parameter-monitoring-in-neonatology-robust-unobtrusive-heart-rate-detection-in-a-realistic-clinical-scenario
#12
Nikolai Blanik, Konrad Heimann, Carina Pereira, Michael Paul, Vladimir Blazek, Boudewijn Venema, Thorsten Orlikowsky, Steffen Leonhardt
Vital parameter monitoring of term and preterm infants during incubator care with self-adhesive electrodes or sensors directly positioned on the skin [e.g. photoplethysmography (PPG) for oxygen saturation or electrocardiography (ECG)] is an essential part of daily routine care in neonatal intensive care units. For various reasons, this kind of monitoring contains a lot of stress for the infants. Therefore, there is a need to measure vital parameters (for instance respiration, temperature, pulse, oxygen saturation) without mechanical or conductive contact...
December 1, 2016: Biomedizinische Technik. Biomedical Engineering
https://www.readbyqxmd.com/read/27720124/enhanced-sensing-of-dengue-virus-dna-detection-using-o2-plasma-treated-silicon-nanowire-based-electrical-biosensor
#13
S F A Rahman, N A Yusof, U Hashim, R Hushiarian, M Nuzaihan M N, M N Hamidon, R M Zawawi, M F M Fathil
Dengue Virus (DENV) has become one of the most serious arthropod-borne viral diseases, causing death globally. The existing methods for DENV detection suffer from the late stage treatment due to antibodies-based detection which is feasible only after five days following the onset of the illness. Here, we demonstrated the highly effective molecular electronic based detection utilizing silicon nanowire (SiNW) integrated with standard complementary metal-oxide-semiconductor (CMOS) process as a sensing device for detecting deoxyribonucleic acid (DNA) related to DENV in an early stage diagnosis...
October 26, 2016: Analytica Chimica Acta
https://www.readbyqxmd.com/read/27704200/prominent-cerebral-veins-on-susceptibility-weighted-imaging-swi-in-pulmonary-embolism
#14
Bilge Öztoprak
OBJECTIVES: Clinical applications of susceptibility-weighted imaging (SWI) are increasing steadily. The aim of this study is to investigate the appearance of cerebral veins on SWI, which is very sensitive to the deoxyhaemoglobin level in vessels, in pulmonary embolism (PE). METHODS: The cranial SWI images of 19 patients with PE and 22 controls from September 2013 through March 2016 were retrospectively examined for the presence of prominent cerebral veins. MRI findings were correlated with blood oxygen levels...
October 4, 2016: European Radiology
https://www.readbyqxmd.com/read/27703196/degradation-of-redox-sensitive-proteins-including-peroxiredoxins-and-dj-1-is-promoted-by-oxidation-induced-conformational-changes-and-ubiquitination
#15
In-Kang Song, Jae-Jin Lee, Jin-Hwan Cho, Jihye Jeong, Dong-Hae Shin, Kong-Joo Lee
Reactive oxygen species (ROS) are key molecules regulating various cellular processes. However, what the cellular targets of ROS are and how their functions are regulated is unclear. This study explored the cellular proteomic changes in response to oxidative stress using H2O2 in dose- and recovery time-dependent ways. We found discernible changes in 76 proteins appearing as 103 spots on 2D-PAGE. Of these, Prxs, DJ-1, UCH-L3 and Rla0 are readily oxidized in response to mild H2O2 stress, and then degraded and active proteins are newly synthesized during recovery...
October 5, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27696646/diet-induced-changes-of-redox-potential-underlie-compositional-shifts-in-the-rumen-archaeal-community
#16
Nir Friedman, Eran Shriker, Ben Gold, Thomer Durman, Raphy Zarecki, Eytan Ruppin, Itzhak Mizrahi
Dietary changes are known to affect gut community structure, but questions remain about the mechanisms by which diet induces shifts in microbiome membership. Here we addressed these questions in the rumen microbiome ecosystem - a complex microbial community that resides in the upper digestive tract of ruminant animals and is responsible for the degradation of the ingested plant material. Our dietary intervention experiments revealed that diet affects the most abundant taxa within the microbiome and that a specific group of methanogenic archaea of the order Methanomicrobiales is highly sensitive to its changes...
October 1, 2016: Environmental Microbiology
https://www.readbyqxmd.com/read/27664046/hypoxia-and-inflammatory-bowel-disease
#17
Eoin P Cummins, Daniel Crean
Inflammatory bowel disease (IBD) is a general term to describe inflammatory diseases of the gastrointestinal tract such as Crohn's disease and ulcerative colitis. IBD affects approximately 1 in 200 individuals and exerts a significant health and quality of life burden on patients. Surgical intervention can be curative in ulcerative colitis but there is currently no cure for Crohn's disease. Since this is the case, and the fact that patients are often diagnosed at a young age, IBD exerts a significant financial burden on the health care system, and society as a whole...
September 20, 2016: Microbes and Infection
https://www.readbyqxmd.com/read/27639094/physiological-genomic-and-transcriptional-diversity-in-responses-to-boron-deficiency-in-rapeseed-genotypes
#18
Yingpeng Hua, Ting Zhou, Guangda Ding, Qingyong Yang, Lei Shi, Fangsen Xu
Allotetraploid rapeseed (Brassica napus L. AnAnCnCn, 2n=4x=38) is highly susceptible to boron (B) deficiency, a widespread limiting factor that causes severe losses in seed yield. The genetic variation in the sensitivity to B deficiency found in rapeseed genotypes emphasizes the complex response architecture. In this research, a B-inefficient genotype, 'Westar 10' ('W10'), responded to B deficiencies during vegetative and reproductive development with an over-accumulation of reactive oxygen species, severe lipid peroxidation, evident plasmolysis, abnormal floral organogenesis, and widespread sterility compared to a B-efficient genotype, 'Qingyou 10' ('QY10')...
October 2016: Journal of Experimental Botany
https://www.readbyqxmd.com/read/27630577/mitochondrial-dna-sequence-and-lack-of-response-to-anoxia-in-the-annual-killifish-austrofundulus-limnaeus
#19
Josiah T Wagner, Florisela Herrejon Chavez, Jason E Podrabsky
The annual killifish Austrofundulus limnaeus inhabits ephemeral ponds in regions of Venezuela, South America. Permanent populations of A. limnaeus are maintained by production of stress-tolerant embryos that are able to persist in the desiccated sediment. Previous work has demonstrated that A. limnaeus have a remarkable ability to tolerate extended periods of anoxia and desiccating conditions. After considering temperature, A. limnaeus embryos have the highest known tolerance to anoxia when compared to any other vertebrate yet studied...
2016: Frontiers in Physiology
https://www.readbyqxmd.com/read/27616641/functional-magnetic-resonance-imaging-of-the-cervical-spinal-cord-during-thermal-stimulation-across-consecutive-runs
#20
Kenneth A Weber, Yufen Chen, Xue Wang, Thorsten Kahnt, Todd B Parrish
The spinal cord is the first site of nociceptive processing in the central nervous system and has a role in the development and perpetuation of clinical pain states. Advancements in functional magnetic resonance imaging are providing a means to non-invasively measure spinal cord function, and functional magnetic resonance imaging may provide an objective method to study spinal cord nociceptive processing in humans. In this study, we tested the validity and reliability of functional magnetic resonance imaging using a selective field-of-view gradient-echo echo-planar-imaging sequence to detect activity induced blood oxygenation level-dependent signal changes in the cervical spinal cord of healthy volunteers during warm and painful thermal stimulation across consecutive runs...
December 2016: NeuroImage
keyword
keyword
83180
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"