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https://www.readbyqxmd.com/read/28443119/parsing-the-regulatory-network-between-small-rnas-and-target-genes-in-ethylene-pathway-in-tomato
#1
Yunxiang Wang, Qing Wang, Lipu Gao, Benzhong Zhu, Zheng Ju, Yunbo Luo, Jinhua Zuo
Small RNAs are a class of short non-coding endogenous RNAs that play essential roles in many biological processes. Recent studies have reported that microRNAs (miRNAs) are also involved in ethylene signaling in plants. LeERF1 is one of the ethylene response factors (ERFs) in tomato that locates in the downstream of ethylene signal transduction pathway. To elucidate the intricate regulatory roles of small RNAs in ethylene signaling pathway in tomato, the deep sequencing and bioinformatics methods were combined to decipher the small RNAs landscape in wild and sense-/antisense-LeERF1 transgenic tomato fruits...
2017: Frontiers in Plant Science
https://www.readbyqxmd.com/read/28402161/ein2-mediated-signaling-is-involved-in-pre-invasion-defense-in-nicotiana-benthamiana-against-potato-late-blight-pathogen-phytophthora-infestans
#2
Soriya Rin, Yuri Mizuno, Yusuke Shibata, Mayuka Fushimi, Shinpei Katou, Ikuo Sato, Sotaro Chiba, Kazuhito Kawakita, Daigo Takemoto
Nicotiana benthamiana ABCG1 and ABCG2 are ABC transporters which are probably involved in the export of capsidiol, the major phytoalexin of Nicotiana species. While capsidiol export by these transporters plays an essential role in post-invasion defense against Phytophthora infestans, they also export unidentified antimicrobial compound(s) involved in pre-invasion defense. In this study, promoter activity of NbABCG2 (Pabcg2a) was analyzed using a GFP marker. Expression of GFP under the control of Pabcg2a was significantly increased by co-expression with the INF1 elicitor from P...
April 12, 2017: Plant Signaling & Behavior
https://www.readbyqxmd.com/read/28400495/constitutively-active-arabidopsis-map-kinase-3-triggers-defense-responses-involving-salicylic-acid-and-summ2-resistance-protein
#3
Baptiste Genot, Julien Lang, Souha Berriri, Marie Garmier, Françoise Gilard, Stéphanie Pateyron, Katrien Haustraete, Dominique Van Der Straeten, Heribert Hirt, Jean Colcombet
Mitogen-activated protein kinases (MAPKs) are important regulators of plant immunity. Most of the knowledge about the function of these pathways is derived from loss-of-function approaches. Using a gain-of-function approach, we investigated the responses controlled by a constitutively active (CA) MPK3 in Arabidopsis thaliana. CA-MPK3 plants are dwarfed and display a massive de-repression of defense genes associated with spontaneous cell death as well as accumulation of reactive oxygen species (ROS), phytoalexins and the stress-related hormones ethylene and salicylic acid (SA)...
April 11, 2017: Plant Physiology
https://www.readbyqxmd.com/read/28388654/triplin-a-small-molecule-reveals-copper-ion-transport-in-ethylene-signaling-from-atx1-to-ran1
#4
Wenbo Li, Randy F Lacey, Yajin Ye, Juan Lu, Kuo-Chen Yeh, Youli Xiao, Laigeng Li, Chi-Kuang Wen, Brad M Binder, Yang Zhao
Copper ions play an important role in ethylene receptor biogenesis and proper function. The copper transporter RESPONSIVE-TO-ANTAGONIST1 (RAN1) is essential for copper ion transport in Arabidopsis thaliana. However it is still unclear how copper ions are delivered to RAN1 and how copper ions affect ethylene receptors. There is not a specific copper chelator which could be used to explore these questions. Here, by chemical genetics, we identified a novel small molecule, triplin, which could cause a triple response phenotype on dark-grown Arabidopsis seedlings through ethylene signaling pathway...
April 2017: PLoS Genetics
https://www.readbyqxmd.com/read/28344560/nitric-oxide-has-a-concentration-dependent-effect-on-the-cell-cycle-acting-via-ein2-in-arabidopsis-thaliana-cultured-cells
#5
Galina V Novikova, Luis A J Mur, Alexander V Nosov, Artem A Fomenkov, Kirill S Mironov, Anna S Mamaeva, Evgeny S Shilov, Victor Y Rakitin, Michael A Hall
Ethylene is known to influence the cell cycle (CC) via poorly characterized roles whilst nitric oxide (NO) has well-established roles in the animal CC but analogous role(s) have not been reported for plants. As NO and ethylene signaling events often interact we examined their role in CC in cultured cells derived from Arabidopsis thaliana wild-type (Col-0) plants and from ethylene-insensitive mutant ein2-1 plants. Both NO and ethylene were produced mainly during the first 5 days of the sub-cultivation period corresponding to the period of active cell division...
2017: Frontiers in Physiology
https://www.readbyqxmd.com/read/28329733/comparison-of-transcriptional-expression-patterns-of-carotenoid-metabolism-in-cabernet-sauvignon-grapes-from-two-regions-with-distinct-climate
#6
Wei-Kai Chen, Ke-Ji Yu, Bin Liu, Yi-Bin Lan, Run-Ze Sun, Qiang Li, Fei He, Qiu-Hong Pan, Chang-Qing Duan, Jun Wang
The downstream flux of carotenoid metabolism in grape berries includes the biosynthesis of norisoprenoids, a group of important aroma compounds, and the production of ABA, a well-known plant hormone. This study focused on the transcriptional profiling comparison of genes participating in the biosynthesis of carotenoids, norisoprenoids, and ABA in Vitis vinifera 'Cabernet Sauvignon' grapes at pea size, veraison, and ripening stages. The grapes were obtained from Changli (CL, eastern China) and Gaotai (GT, western China) regions and analyzed using RNA-sequencing technology...
March 9, 2017: Journal of Plant Physiology
https://www.readbyqxmd.com/read/28301712/hypoxia-responsive-erfs-involved-in-post-deastringency-softening-of-persimmon-fruit
#7
Miao-Miao Wang, Qing-Gang Zhu, Chu-Li Deng, Zheng-Rong Luo, Ning-Jing Sun, Donald Grierson, Xue-Ren Yin, Kun-Song Chen
Removal of astringency by endogenously formed acetaldehyde, achieved by postharvest anaerobic treatment, is of critical importance for many types of persimmon fruit. Although an anaerobic environment accelerates de-astringency it also has the deleterious effect of promoting excessive softening, reducing shelf-life and marketability. Some hypoxia-responsive ethylene response factor (ERFs) participate in anaerobic de-astringency, but their role in accelerated softening was unclear. Undesirable rapid softening induced by high CO2 (95%) was ameliorated by adding the ethylene inhibitor 1-MCP (1 μl L(-1) ), resulting in reduced astringency while maintaining firmness, suggesting that CO2 -induced softening involves ethylene signalling...
March 16, 2017: Plant Biotechnology Journal
https://www.readbyqxmd.com/read/28300216/ein3-and-sos2-synergistically-modulate-plant-salt-tolerance
#8
Ruidang Quan, Juan Wang, Dexin Yang, Haiwen Zhang, Zhijin Zhang, Rongfeng Huang
Ethylene biosynthesis and the ethylene signaling pathway regulate plant salt tolerance by activating the expression of downstream target genes such as those related to ROS and Na(+)/K(+) homeostasis. The Salt Overly Sensitive (SOS) pathway regulates Na(+)/K(+) homeostasis in Arabidopsis under salt stress. However, the connection between these two pathways is unclear. Through genetic screening, we identified two sos2 alleles as salt sensitive mutants in the ein3-1 background. Neither Ethylene-Insensitive 2 (EIN2) nor EIN3 changed the expression patterns of SOS genes including SOS1, SOS2, SOS3 and SOS3-like Calcium Binding Protein 8 (SCaBP8), but SOS2 activated the expression of one target gene of EIN3, Ethylene and Salt-inducible ERF 1 (ESE1)...
March 16, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28293853/assessing-attraction-of-nematodes-to-host-roots-using-pluronic-gel-medium
#9
Valerie M Williamson, Rasa Čepulytė
Nematodes and other organisms perceive and respond to plant root exudates. These exudates are affected by the condition and genetic makeup of the plant. Attraction of the root-knot nematode Meloidogyne hapla to roots is altered in plants with mutations affecting ethylene signaling, suggesting that the root exudates to which the nematode responds are modulated by ethylene signaling. Nematode interactions with roots have been difficult to observe directly due to the opaqueness of soil. A medium based on the block copolymer Pluronic F-127 has been useful for studying these interactions...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28293850/analysis-of-growth-and-molecular-responses-to-ethylene-in-etiolated-rice-seedlings
#10
Biao Ma, Jin-Song Zhang
Ethylene plays a key role in various submergence responses of rice plants, but the mechanism of ethylene action remains largely unclear in rice. Regarding the differences between rice and Arabidopsis in ethylene-regulated processes, rice plants may possess divergent mechanisms in ethylene signaling in addition to the conserved aspects. Forward genetic analysis is essential to fully understand the ethylene signaling mechanism in rice. Here, we describe a method for screening ethylene-response mutants and evaluating ethylene responsiveness in etiolated rice seedlings...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28293846/circular-dichroism-and-fluorescence-spectroscopy-to-study-protein-structure-and-protein-protein-interactions-in-ethylene-signaling
#11
Mareike Kessenbrock, Georg Groth
Circular dichroism (CD) spectroscopy is an invaluable technique to analyze secondary structure and functional folding of recombinant purified proteins. CD spectroscopy can also be applied to detect changes in protein secondary structure related to the pH or redox conditions found in different cellular compartments or to the interaction with other molecules. Another biophysical technique to monitor conformational changes and interaction with small molecule ligands or biological macromolecules is protein fluorescence spectroscopy making use of the aromatic amino acid tryptophan as a sensitive intrinsic fluorescent probe...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28293845/kinase-assay-for-constitutive-triple-response-1-ctr1-in-arabidopsis-thaliana
#12
Han Yong Lee, Gyeong Mee Yoon
Protein kinases are central components of signal transduction pathways in the cell. They catalyze the phosphorylation of substrate proteins, resulting in changes of the activity, localization, stability, and protein interactions of the substrates, ultimately coordinating the activity of important cellular processes. CONSTITUTIVE TRIPLE RESPONSE 1 (CTR1) is a Raf-like protein kinase that functions as a negative regulator in the phytohormone ethylene signaling pathway. CTR1 physically interacts with ethylene receptors via its N-terminal domain at the endoplasmic reticulum, and is involved in suppressing ethylene signaling in the absence of ethylene...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28293844/localization-of-the-ethylene-receptor-signaling-complex-to-the-endoplasmic-reticulum-analysis-by-two-phase-partitioning-and-density-gradient-centrifugation
#13
G Eric Schaller
Ethylene receptors and other elements of the ethylene-signal transduction pathway localize to membranes of the endoplasmic reticulum (ER). New players in the ethylene signaling pathway continue to be discovered and so it is important to have methods by which to diagnose their cellular localization. Two methods for microsome isolation and fractionation are described here that can assist in determining if a protein localizes to the ER: aqueous two-phase partitioning and equilibrium density-gradient centrifugation...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28293843/analysis-of-ethylene-receptor-interactions-by-co-immunoprecipitation-assays
#14
Zhiyong Gao, G Eric Schaller
Ethylene receptors are predominantly localized to the endoplasmic reticulum (ER) membrane, and coordinate ethylene signal output through protein-protein interactions with each other and additional signaling components. Here, we describe a co-immunoprecipitation (Co-IP) assay based on the use of the Tandem Affinity Purification (TAP) tag to examine the interactions of ethylene receptors in plant extracts. Human IgG-agarose beads are used to pull down TAP-tagged versions of the protein of interest from detergent extracts of Arabidopsis membranes, and the precipitate then is analyzed immunologically for co-purification of the ethylene receptors...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28224919/characteristics-and-regulatory-pathway-of-the-prupesep1-sepallata-gene-during-ripening-and-softening-in-peach-fruits
#15
Jinjin Li, Fang Li, Ming Qian, Mingyu Han, Hangkong Liu, Dong Zhang, Juanjuan Ma, Caiping Zhao
SEPALLATA genes are members of a subfamily of MADS-box transcription factors, and have essential roles in floral organ development and fruit ripening. In this study, the PrupeSEP1 gene was cloned from peach flesh. Its deduced amino acid sequence was very similar to that of MdMADS8 and MdMADS9 in apple and MADS-RIN-like in strawberry. During storage of melting flesh (MF) peach, the pattern of SEP1 expression was similar to that of ethylene biosynthesis and ethylene signal transduction-related gene expression (EIN2 and ETR2)...
April 2017: Plant Science: An International Journal of Experimental Plant Biology
https://www.readbyqxmd.com/read/28167024/a-novel-ethylene-responsive-factor-citerf13-plays-a-role-in-photosynthesis-regulation
#16
Xiu-Lan Xie, Xiao-Jian Xia, Sheng Kuang, Xi-Li Zhang, Xue-Ren Yin, Jing-Quan Yu, Kun-Song Chen
Ethylene responsive factors (ERFs) act as critical downstream components of the ethylene signalling pathway in regulating plant development and stress responses. However little is known about its role in regulation of photosynthesis. Here, we identified an ethylene-inducible ERF gene in citrus, CitERF13. Transient over-expression of CitERF13 in N. tabacum leaves, resulted in a significant decrease in net photosynthetic rate. Closer examination of photosynthetic activity of PSII and PSI indicated that CitERF13 overexpression led to declines of Fv/Fm, Y(II) and Y(I)...
March 2017: Plant Science: An International Journal of Experimental Plant Biology
https://www.readbyqxmd.com/read/28130221/gene-expression-analysis-in-musa-acuminata-during-compatible-interactions-with-meloidogyne-incognita
#17
Nancy Eunice Niño Castañeda, Gabriel Sergio Costa Alves, Rosane Mansan Almeida, Edson Perito Amorim, Claudia Fortes Ferreira, Roberto Coiti Togawa, Marcos Mota Do Carmo Costa, Priscila Grynberg, Jansen Rodrigo Pereira Santos, Juvenil Enrique Cares, Robert Neil Gerard Miller
Background and Aims: Endoparasitic root-knot nematodes (RKNs) ( Meloidogyne spp.) cause considerable losses in banana ( Musa spp.), with Meloidogyne incognita a predominant species in Cavendish sub-group bananas. This study investigates the root transcriptome in Musa acuminata genotypes 4297-06 (AA) and Cavendish Grande Naine (CAV; AAA) during early compatible interactions with M. incognita . Methods: Roots were analysed by brightfield light microscopy over a 35 d period to examine nematode penetration and morphological cell transformation...
March 1, 2017: Annals of Botany
https://www.readbyqxmd.com/read/28118091/paraburkholderia-phytofirmans-psjn-protects-arabidopsis-thaliana-against-a-virulent-strain-of-pseudomonas-syringae-through-the-activation-of-induced-resistance
#18
Tania Timmermann, Grace Armijo, Raúl Donoso, Aldo Seguel, Loreto Holuigue, Bernardo González
Paraburkholderia phytofirmans PsJN is a plant growth-promoting rhizobacterium (PGPR) that stimulates plant growth and improves tolerance to abiotic stresses. This study analyzed whether strain PsJN can reduce plant disease severity and proliferation of the virulent strain Pseudomonas syringae pv. tomato DC3000, in Arabidopsis plants, through the activation of induced resistance. Arabidopsis plants previously exposed to strain PsJN showed a reduction in disease severity and pathogen proliferation in leaves compared with noninoculated, infected plants...
March 16, 2017: Molecular Plant-microbe Interactions: MPMI
https://www.readbyqxmd.com/read/28112434/label-free-deep-shotgun-proteomics-reveals-protein-dynamics-during-tomato-fruit-tissues-development
#19
Jedrzej Szymanski, Yishai Levin, Alon Savidor, Dario Breitel, Louise Chappell-Maor, Uwe Heinig, Nadine Töpfer, Asaph Aharoni
Current innovations in mass-spectrometry-based technologies allow deep coverage of protein expression. Despite its immense value and in contrast to transcriptomics, only a handful of studies in crop plants engaged with global proteome assays. Here, we present large-scale shotgun proteomics profiling of tomato fruit across two key tissues and five developmental stages. A total of 7738 individual protein groups were identified and reliably measured at least in one of the analyzed tissues or stages. The depth of our assay enabled identification of 61 differentially expressed transcription factors, including renowned ripening-related regulators and elements of ethylene signaling...
January 23, 2017: Plant Journal: for Cell and Molecular Biology
https://www.readbyqxmd.com/read/28073951/nod-factors-potentiate-auxin-signaling-for-transcriptional-regulation-and-lateral-root-formation-in-medicago-truncatula
#20
Violaine Herrbach, Ximena Chirinos, David Rengel, Kokoévi Agbevenou, Rémy Vincent, Stéphanie Pateyron, Stéphanie Huguet, Sandrine Balzergue, Asher Pasha, Nicholas Provart, Clare Gough, Sandra Bensmihen
Nodulation (Nod) factors (NFs) are symbiotic molecules produced by rhizobia that are essential for establishment of the rhizobium-legume endosymbiosis. Purified NFs can stimulate lateral root formation (LRF) in Medicago truncatula, but little is known about the molecular mechanisms involved. Using a combination of reporter constructs, pharmacological and genetic approaches, we show that NFs act on early steps of LRF in M. truncatula, independently of the ethylene signaling pathway and of the cytokinin receptor MtCRE1, but in interaction with auxin...
January 1, 2017: Journal of Experimental Botany
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