keyword
MENU ▼
Read by QxMD icon Read
search

degradation

keyword
https://www.readbyqxmd.com/read/28817833/fmnl2-destabilises-commd10-to-activate-nf-%C3%AE%C2%BAb-pathway-in-invasion-and-metastasis-of-colorectal-cancer
#1
S S Yang, X M Li, M Yang, X L Ren, J L Hu, X H Zhu, F F Wang, Z C Zeng, J Y Li, Z Q Cheng, W T Liao, Y Q Ding, J Guan, L Liang
BACKGROUND: Diaphanous-related formins (DRFs), actin necleator, have been known to participate in the progression of cancer cells. We previously reported that FMNL2 (Formin-like2), a member of DRFs, was a positive regulator in colorectal cancer (CRC) metastasis, yet proteins and pathways required for the function of this pro-invasive DRFs remain to be identified. METHODS: The relationship between FMNL2 and COMMD10 was examined using Co-IP, GST pull-down, immunofluorescence and in vitro ubiquitination assay...
August 17, 2017: British Journal of Cancer
https://www.readbyqxmd.com/read/28817810/tgf-%C3%AE-1-is-involved-in-vitamin-d-induced-chondrogenic-differentiation-of-bone-marrow-derived-mesenchymal-stem-cells-by-regulating-the-erk-jnk-pathway
#2
Xiaorui Jiang, Botao Huang, Huiying Yang, Guishi Li, Chunlei Zhang, Guangshi Yang, Feng Lin, Guodong Lin
BACKGROUND/AIMS: Osteoarthritis (OA) is characterized by degradation of cartilage, sole cell type of which is chondrocytes. Bone marrow-derived mesenchymal stem cells (BMSCs) possess multipotency and can be directionally differentiated into chondrocytes under stimulation. This study was aimed to explore the possible roles of vitamin D and transforming growth factor-β1 (TGF-β1) in the chondrogenic differentiation of BMSCs. METHODS: BMSCs were isolated from femurs and tibias of rats and characterized by flow cytometry...
August 16, 2017: Cellular Physiology and Biochemistry
https://www.readbyqxmd.com/read/28817705/cell-surface-dynamics-and-cellular-distribution-of-endogenous-fcrn
#3
Lena D'Hooghe, Andrew D Chalmers, Sam Heywood, Paul Whitley
A major role for FcRn is the salvage of pinocytosed IgG and albumin from a degradative fate in lysosomes. FcRn achieves this by binding IgG in a pH-dependent manner in acidic endosomes and recycling it to the plasma membrane to be released at neutral pH. This is important in maintaining high serum IgG and albumin levels and has the potential to be exploited to modulate the pharmacokinetics of antibody-based therapeutics. Although FcRn is responsible for the recycling of IgG, the dynamic behaviour of endogenous FcRn is not well understood...
2017: PloS One
https://www.readbyqxmd.com/read/28817702/global-gene-expression-analysis-of-the-response-of-physic-nut-jatropha-curcas-l-to-medium-and-long-term-nitrogen-deficiency
#4
Qi Kuang, Sheng Zhang, Pingzhi Wu, Yaping Chen, Meiru Li, Huawu Jiang, Guojiang Wu
Jatropha curcas L. is an important biofuel plant with excellent tolerance of barren environments. However, studies on the regulatory mechanisms that operate in this plant in response to nitrogen (N) shortage are scarce. In this study, genome-wide transcriptional profiles of the roots and leaves of 8-week old physic nut seedlings were analyzed after 2 and 16 days of N starvation. Enrichment results showed that genes associated with N metabolism, processing and regulation of RNA, and transport predominated among those showing alterations in expression...
2017: PloS One
https://www.readbyqxmd.com/read/28817671/immunohistochemical-analysis-reveals-variations-in-proteasome-tissue-expression-in-c-elegans
#5
Elisa Mikkonen, Caj Haglund, Carina I Holmberg
The ubiquitin-proteasome system (UPS) plays a crucial part in normal cell function by mediating intracellular protein clearance. We have previously shown that UPS-mediated protein degradation varies in a cell type-specific manner in C. elegans. Here, we use formalin-fixed, paraffin-embedded C. elegans sections to enable studies on endogenous proteasome tissue expression. We show that the proteasome immunoreactivity pattern differs between cell types and within subcellular compartments in adult wild-type (N2) C...
2017: PloS One
https://www.readbyqxmd.com/read/28817657/metaproteomics-analysis-of-the-functional-insights-into-microbial-communities-of-combined-hydrogen-and-methane-production-by-anaerobic-fermentation-from-reed-straw
#6
Xuan Jia, Bei-Dou Xi, Ming-Xiao Li, Yang Yang, Yong Wang
A metaproteomic approach was used to analyse the proteins expressed and provide functional evidence of key metabolic pathways in the combined production of hydrogen and methane by anaerobic fermentation (CHMP-AF) for reed straw utilisation. The functions and structures of bacteria and archaea populations show significant succession in the CHMP-AF process. There are many kinds of bacterial functional proteins, mainly belonging to phyla Firmicutes, Proteobacteria, Actinobacteria and Bacteroidetes, that are involved in carbohydrate metabolism, energy metabolism, lipid metabolism, and amino acid metabolism...
2017: PloS One
https://www.readbyqxmd.com/read/28817614/development-of-a-technique-for-psyllium-husk-mucilage-purification-with-simultaneous-microencapsulation-of-curcumin
#7
André Álvares Monge Neto, Rita de Cássia Bergamasco, Flávio Faria de Moraes, Antônio Medina Neto, Rosane Marina Peralta
This study focused on evaluating a technique for the psyllium husk mucilage (PHM) purification with simultaneous microencapsulation of curcumin. PHM was extracted with water and purified with ethanol. For the mucilage purification and simultaneous microencapsulation, an ethanolic solution of curcumin was used. After dehydration, the samples were analysed by instrumental techniques and evaluated for thermal stability. The presence of curcumin in the solution did not impair the yield of precipitated polysaccharide...
2017: PloS One
https://www.readbyqxmd.com/read/28817428/interprofessional-qi-training-enhances-competency-and-qi-productivity-among-graduates-findings-from-nationwide-children-s-hospital
#8
Thomas Bartman, Karen Heiser, Andrew Bethune, Wallace Crandall, Richard McClead, J Terrance Davis, Richard J Brilli
PURPOSE: Significant resources are expended on quality improvement (QI) training courses. The authors sought to determine whether education provided in QI course training improves self-assessed QI content competence and QI-related productivity among course graduates. METHOD: "Quality Improvement Essentials" is a four-month didactic and experiential course designed to prepare multidisciplinary professionals to participate in and lead QI efforts at Nationwide Children's Hospital (NCH)...
August 16, 2017: Academic Medicine: Journal of the Association of American Medical Colleges
https://www.readbyqxmd.com/read/28817274/notch-inhibitors-from-calotropis-gigantea-that-induce-neuronal-differentiation-of-neural-stem-cells
#9
Tatsuro Yoneyama, Midori A Arai, Ryuta Akamine, Kazune Koryudzu, Anna Tsuchiya, Samir K Sadhu, Firoj Ahmed, Motoyuki Itoh, Ryuichi Okamoto, Masami Ishibashi
Neurodegenerative diseases such as Alzheimer's disease and Parkinson's disease occur due to loss of the structure and function of neurons. For the potential treatment of neurodegenerative diseases, accelerators of neuronal differentiation of neural stem cells (NSCs) have been focused on and a cell-based assay system for measuring Notch signaling pathway activity was constructed. Using this assay system, eight compounds isolated from Calotropis gigantea were identified as inhibitors of the Notch signaling pathway...
August 17, 2017: Journal of Natural Products
https://www.readbyqxmd.com/read/28817270/magnetic-field-activated-sensing-of-mrna-in-living-cells
#10
Saira F Bakshi, Nataliia Guz, Andrey Zakharchenko, Han Deng, Alexei Tumanov, Craig D Woodworth, Sergiy Minko, Dmitry M Kolpashchikov, Evgeny Katz
Detection of specific mRNA in living cells has attracted significant attention in the last decade. Probes that can be easily delivered into cells and activated at the desired time can contribute to understand-ing translation, trafficking and degradation of mRNA. Here we report a new strategy termed Magnetic Field-Activated Binary De-oxyribozyme (MaBiDZ) sensor that enables both efficient delivery and temporal control of mRNA sensing by magnetic field. MaBiDZ uses two species of magnetic beads conjugated with different com-ponents of a multicomponent deoxyribozyme (DZ) sensor...
August 17, 2017: Journal of the American Chemical Society
https://www.readbyqxmd.com/read/28817267/flavoring-chemicals-and-aldehydes-in-e-cigarette-emissions
#11
Skylar Klager, Jose Vallarino, Piers MacNaughton, David C Christiani, Quan Lu, Joseph G Allen
Regulations on e-cigarettes in the U.S. do not provide guidelines on the chemical content of e-cigarette liquids. We evaluated emissions of aldehydes and flavoring chemicals in e-cigarette vapor under typical usage conditions. We selected 24 e-cigarette flavors from the top selling disposable e-cigarette brands. E-cigarettes were connected to a pump drawing air for two second puffs with sixty-second intervals between puffs. The vapor was analyzed for the presence of aldehydes using high-performance liquid chromatography-ultraviolet detector and for the presence of flavoring chemicals with gas chromatography and an electron capture detector...
August 17, 2017: Environmental Science & Technology
https://www.readbyqxmd.com/read/28817181/introduction-of-hsa-mir-103a-and-hsa-mir-1827-and-hsa-mir-137-as-new-regulators-of-wnt-signaling-pathway-and-their-relation-to-colorectal-carcinoma
#12
Ali Fasihi, Bahram M Soltani, Amir Atashi, Shirzad Nasiri
Wnt signaling is hyper-activated in most of human cancers including colorectal carcinoma (CRC). Therefore, the introduction of new regulators for Wnt pathway possesses promising diagnostic and therapeutic applications in cancer medicine. Bioinformatics analysis introduced hsa-miR-103a, hsa-miR-1827, and hsa-miR-137 as potential regulators of Wnt signaling pathway. Here, we intended to examine the effect of these human miRNAs on Wnt signaling pathway components, on the cell cycle progression in CRC originated cell lines and their expression in CRC tissues...
August 17, 2017: Journal of Cellular Biochemistry
https://www.readbyqxmd.com/read/28817106/ag-coated-heterostructures-of-zno-tio%C3%A2-delaminated-montmorillonite-as-solar-photocatalysts
#13
Carolina Belver, Mariana Hinojosa, Jorge Bedia, Montserrat Tobajas, Maria Ariadna Alvarez, Vicente Rodríguez-González, Juan Jose Rodriguez
Heterostructures based on ZnO-TiO₂/delaminated montmorillonite coated with Ag have been prepared by sol-gel and photoreduction procedures, varying the Ag and ZnO contents. They have been thoroughly characterized by XRD, WDXRF, UV-Vis, and XPS spectroscopies, and N₂ adsorption, SEM, and TEM. In all cases, the montmorillonite was effectively delaminated with the formation of TiO₂ anatase particles anchored on the clay layer's surface, yielding porous materials with high surface areas. The structural and textural properties of the heterostructures synthesized were unaffected by the ZnO incorporated...
August 17, 2017: Materials
https://www.readbyqxmd.com/read/28817091/production-of-synthetic-phosphoanhydrite-and-its-use-as-a-binder-in-self-leveling-underlayments-slu
#14
Cecília Ogliari Schaefer, Malik Cheriaf, Janaíde Cavalcante Rocha
An experimental study was conducted to investigate the potential use of phosphogypsum (PG) to produce self-leveling underlayments. The study was designed in two stages. Initially a phosphoanhydrite (PA) was produced by heating phosphogypsum at temperatures of 350 °C, 450 °C, 550 °C, and 650 °C. Two periods of heating were applied (2 and 4 h). The formation of anhydrite was determined by thermogravimetric analysis (DTA-TG) and confirmed by X-ray diffraction (XRD). The results show that anhydrite II was obtained at temperatures above 450 °C, and at higher calcination temperatures the PA solubility was lower...
August 17, 2017: Materials
https://www.readbyqxmd.com/read/28817071/topological-interference-management-for-k-user-downlink-massive-mimo-relay-network-channel
#15
Poongundran Selvaprabhu, Sunil Chinnadurai, Jun Li, Moon Ho Lee
In this paper, we study the emergence of topological interference alignment and the characterizing features of a multi-user broadcast interference relay channel. We propose an alternative transmission strategy named the relay space-time interference alignment (R-STIA) technique, in which a K -user multiple-input-multiple-output (MIMO) interference channel has massive antennas at the transmitter and relay. Severe interference from unknown transmitters affects the downlink relay network channel and degrades the system performance...
August 17, 2017: Sensors
https://www.readbyqxmd.com/read/28816606/scale-up-of-puf-immobilized-fungal-chitosanase-lipase-preparation-production
#16
Katarzyna Struszczyk-Świta, Łukasz Stańczyk, Mirosława Szczęsna-Antczak, Tadeusz Antczak
Mucor circinelloides IBT-83 mycelium that exhibits both lipolytic (AL) and chitosanolytic (ACH) activities was immobilized into polyurethane foam (PUF) in a 30 L laboratory fermenter. The process of immobilization was investigated in terms of: the carrier porosity, its type, amount and shape, location inside the fermenter, mixing and aeration parameters during the culture, as well as downstream processing operations. The selected conditions allowed for immobilization of approx. 7 g of defatted and dried mycelium in 1 g of carrier, i...
August 17, 2017: Preparative Biochemistry & Biotechnology
https://www.readbyqxmd.com/read/28816416/offsetting-global-warming-induced-elevated-greenhouse-gas-emissions-from-an-arable-soil-by-biochar-application
#17
Chris Bamminger, Christian Poll, Sven Marhan
Global warming will likely enhance greenhouse gas (GHG) emissions from soils. Due to its slow decomposability, biochar is widely recognized as effective in long-term soil carbon (C) sequestration and in mitigation of soil GHG emissions. In a long-term soil warming experiment (+2.5 °C, since July 2008) we studied the effect of applying high-temperature Miscanthus biochar (0, 30 t ha(-1) , since August 2013) on GHG emissions and their global warming potential (GWP) during two years in a temperate agroecosystem...
August 17, 2017: Global Change Biology
https://www.readbyqxmd.com/read/28816384/intracellular-delivery-of-functional-native-antibodies-under-hypoxic-conditions-by-using-a-biodegradable-silica-nanoquencher
#18
Shao Q Yao, Peiyan Yuan, Hailong Zhang, Linghui Qian, Xin Mao, Shubo Du, Changmin Yu, Bo Peng
Antibodies are important biopharmaceuticals, but almost all antibody-based drugs are limited to targeting antigens located at the cell exterior due to their inability to enter the cell interior. Available methods for intracellular delivery of antibodies have major shortcomings. Herein we report an approach to encapsulate native antibodies in a biodegradable silica nanoquencher (BS-qNP) which could undergo efficient cellular uptake and intracellular degradation to release antibodies under hypoxic conditions...
August 17, 2017: Angewandte Chemie
https://www.readbyqxmd.com/read/28816361/msel-1l-deficiency-affects-vasculogenesis-and-neural-stem-cell-lineage-commitment
#19
Cardano M, Diaferia G R, Conti L, Baronchelli S, Sessa A, Broccoli V, Barbieri A, De Blasio P, Biunno I
mSEL-1L is a highly conserved ER-resident type I protein, involved in the degradation of misfolded peptides through the ubiquitin-proteasome system (UPS), a pathway known to control the plasticity of the vascular smooth muscle cells (VSMC) phenotype and survival. In this article we demonstrate that mSEL-1L deficiency interferes with the murine embryonic vascular network, showing particular irregularities in the intracranic and intersomitic neurovascular units and in the cerebral capillary microcirculation. During murine embryogenesis, mSEL-1L is expressed in cerebral areas known to harbor progenitor neural cells, while in the adult brain the protein is specifically restricted to the stem cell niches, co-localizing with Sox2 and Nestin...
August 17, 2017: Journal of Cellular Physiology
https://www.readbyqxmd.com/read/28816350/characteristics-and-functionality-of-appetite-reducing-thylakoid-powders-produced-by-three-different-drying-processes
#20
Karolina Östbring, Ingegerd Sjöholm, Henrietta Sörenson, Andrej Ekholm, Charlotte Erlanson-Albertsson, Marilyn Rayner
BACKGROUND: Thylakoids, a chloroplast membrane extracted from green leaves, is a promising functional ingredient with appetite reducing properties via their lipase-inhibiting effect. Thylakoids in powder form has been evaluated in animal and human models but no comprehensive study has been conducted on powder characteristics. The aim was to investigate the effects of different isolation methods and drying techniques (drum-drying, spray-drying, freeze-drying) on thylakoids' physicochemical and functional properties...
August 17, 2017: Journal of the Science of Food and Agriculture
keyword
keyword
83135
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"