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https://www.readbyqxmd.com/read/26109017/dmek-lenticule-preparation-using-an-air-dissection-technique-central-versus-peripheral-injection
#1
COMPARATIVE STUDY
Sepehr Feizi, Mohammad Ali Javadi
PURPOSE: To compare 2 sites of air injection to prepare donor lenticules for Descemet membrane (DM) endothelial keratoplasty. METHODS: Fifty-one human corneoscleral buttons from donors aged 4 to 57 years were used. Corneoscleral buttons were placed endothelial side up and a 27-G needle was inserted just outside Schwalbe line and advanced immediately beneath the endothelium. Air was injected centrally inside the 8-mm zone (group 1) or peripherally approximately 1 mm in front of the limbus (group 2)...
January 2016: European Journal of Ophthalmology
https://www.readbyqxmd.com/read/23289492/phenotypic-findings-in-c1qtnf5-retinopathy-late-onset-retinal-degeneration
#2
Vasileios Soumplis, Panagiotis I Sergouniotis, Anthony G Robson, Michel Michaelides, Anthony T Moore, Graham E Holder, Andrew R Webster
PURPOSE: To describe the ocular and electrophysiological phenotype of four patients with late-onset retinal degeneration (LORD). METHODS: Clinical examination, fundus and anterior segment photography, fundus autofluorescence imaging and spectral domain optical coherence tomography (SD-OCT) were performed. Three patients underwent pattern and full-field electroretinography (ERG). Patient DNA was screened for the c.686C>G, p.Ser163Arg mutation in C1QTNF5. RESULTS: All affected individuals had a family history suggestive of autosomal dominant inheritance with full penetrance...
May 2013: Acta Ophthalmologica
https://www.readbyqxmd.com/read/21490574/organotypic-slice-culture-of-gfp-expressing-mouse-embryos-for-real-time-imaging-of-peripheral-nerve-outgrowth
#3
Isabel Brachmann, Kerry L Tucker
For many purposes, the cultivation of mouse embryos ex vivo as organotypic slices is desirable. For example, we employ a transgenic mouse line (tauGFP) in which the enhanced version of the green fluorescent protein (EGFP) is exclusively expressed in all neurons of the developing central and peripheral nervous system(1), allowing the possibility to both film the innervation of the forelimb and to manipulate this process with pharmacological and genetic techniques(2). The most critical parameter in the successful cultivation of such slice cultures is the method by which the slices are prepared...
2011: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/20018688/autoreactivity-in-an-hiv-1-broadly-reactive-neutralizing-antibody-variable-region-heavy-chain-induces-immunologic-tolerance
#4
Laurent Verkoczy, Marilyn Diaz, T Matt Holl, Ying-Bin Ouyang, Hilary Bouton-Verville, S Munir Alam, Hua-Xin Liao, Garnett Kelsoe, Barton F Haynes
We previously reported that some of the rare broadly reactive, HIV-1 neutralizing antibodies are polyreactive, leading to the hypothesis that induction of these types of neutralizing antibody may be limited by immunologic tolerance. However, the notion that such antibodies are sufficiently autoreactive to trigger B cell tolerance is controversial. To test directly whether rare neutralizing HIV-1 antibodies can activate immunologic tolerance mechanisms, we generated a knock-in mouse in which the Ig heavy chain (HC) variable region rearrangement (V(H)DJ(H)) from the polyreactive and broadly neutralizing human monoclonal antibody 2F5 was targeted into the mouse Igh locus...
January 5, 2010: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/15515767/-to-establish-and-observe-the-experimental-lens-regeneration-model-in-rabbits
#5
Xialin Liu, Xinyu Zhang, Yizhi Liu, Xinhua Liu
PURPOSE: To establish the lens regeneration model in New Zealand rabbits, observe the lens regeneration process to investigate the mechanism of lens epithelial cell growth and lens regeneration. METHODS: Lens extraction by endocapsular phacoemulsification was performed on 8 experimental eyes in New Zealand albino rabbits weighing approximately 1.5 - 2.5 kg. To restore a relative intact capsule bag, a little needle tip was inserted through the limbal wound to perform a 2 mm-lined anterior capsulotomy, which just permitted the insertion of a 21G phacoemulsification tip...
December 2002: Yan Ke Xue Bao, Eye Science
https://www.readbyqxmd.com/read/11287583/multiple-immediate-early-gene-deficient-herpes-simplex-virus-vectors-allowing-efficient-gene-delivery-to-neurons-in-culture-and-widespread-gene-delivery-to-the-central-nervous-system-in-vivo
#6
C E Lilley, F Groutsi, Z Han, J A Palmer, P N Anderson, D S Latchman, R S Coffin
Herpes simplex virus (HSV) has several potential advantages as a vector for delivering genes to the nervous system. The virus naturally infects and remains latent in neurons and has evolved the ability of highly efficient retrograde transport from the site of infection at the periphery to the site of latency in the spinal ganglia. HSV is a large virus, potentially allowing the insertion of multiple or very large transgenes. Furthermore, HSV does not integrate into the host chromosome, removing any potential for insertional activation or inactivation of cellular genes...
May 2001: Journal of Virology
https://www.readbyqxmd.com/read/10908315/the-d2s-dopamine-receptor-stimulates-phospholipase-d-activity-a-novel-signaling-pathway-for-dopamine
#7
S E Senogles
The D2 dopamine receptor isoforms signal to a variety of cellular effectors in both the central nervous system and periphery. Two alternative splice forms of the D2 dopamine receptor exist, the D2s (short) and D2l (long), which has an insertion of 29 amino acids in the third intracellular loop (). In cells of the anterior lobe of the pituitary, D2 dopamine receptors (both forms) are present on lactotroph cells coupled to the inhibition of adenylyl cyclase, activation of voltage-gated calcium channels, and inhibition of potassium channels...
August 2000: Molecular Pharmacology
https://www.readbyqxmd.com/read/9665811/genes-expressed-after-retinoic-acid-mediated-differentiation-of-embryoid-bodies-are-likely-to-be-expressed-during-embryo-development
#8
S Gajović, K Chowdhury, P Gruss
In order to test if retinoic acid-mediated differentiation of embryoid bodies can be used as an in vitro preselection method for ES cell lines generated by gene trap, we correlated gene expression after in vitro differentiation and in 11.5-day embryos. Fifty-two genes captured by gene trap and expressed in undifferentiated embryonic stem cells were analyzed. Most genes expressed after differentiation in vitro were also expressed during embryo development. In order to correlate the expression patterns in vitro and in vivo, the in vitro expression in the center and in the periphery of the embryoid body outgrowths was observed...
July 10, 1998: Experimental Cell Research
https://www.readbyqxmd.com/read/6541556/polygonal-arrays-of-microfilaments-in-epithelial-cells-of-the-intact-lens
#9
COMPARATIVE STUDY
N S Rafferty, D L Scholz
Polygonal arrays of microfilaments have been discovered to line the inner apical plasma membrane of anterior epithelial cells of the intact rabbit lens. When tangential sections are studied with the electron microscope, the polygonal arrays are seen to consist of central vertices interconnected by rays of filaments. The rays near the cell periphery insert into the lateral plasma membrane. The vertices are spaced about 1 micron apart and appear to be attached to the apical plasma membrane. The polygonal arrays have little depth as judged by stereo-pairs and are incorporated within the dense band of microfilaments seen in cross-section at the epithelio-fiber junction...
September 1984: Current Eye Research
https://www.readbyqxmd.com/read/632321/microtubular-apparates-of-melanophores-three-dimensional-organization
#10
M Schliwa
Microtubular organization in the melanophores of the angelfish, Pterophyllum scalare, has been studied by serial thin sectioning. The course of microtubules has been followed in sets of transverse serial sections taken from the centrosphere and a segment of a cell process, respectively. Microtubules arise from a prominent zone in the cell center, the central apparatus, which is composed of numerous, small, electron-dense aggregates. the number of these loosely distributed densities is highest in the center of the centrosphere, but they may also be found at its periphery...
March 1978: Journal of Cell Biology
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