keyword
MENU ▼
Read by QxMD icon Read
search

Kenn

keyword
https://www.readbyqxmd.com/read/28214373/improved-detection-of-zika-virus-rna-in-human-and-animal-specimens-by-a-novel-highly-sensitive-and-specific-real-time-rt-pcr-assay-targeting-the-5-untranslated-region-of-zika-virus
#1
Jasper Fuk-Woo Chan, Cyril Chik-Yan Yip, Kah-Meng Tee, Zheng Zhu, Jessica Oi-Ling Tsang, Kenn Ka-Heng Chik, Terance Gi-Wai Tsang, Chris Chung-Sing Chan, Vincent Kwok-Man Poon, Siddharth Sridhar, Feifei Yin, Ivan Fan-Ngai Hung, Sandy Ka-Yee Chau, Anna Jinxia Zhang, Kwok-Hung Chan, Kwok-Yung Yuen
OBJECTIVE AND METHOD: We developed and evaluated five novel real-time RT-PCR assays targeting conserved regions in the 5'-untranslated region (5'-UTR), envelope (E'), non-structural protein 2A (NS2A), NS5, and 3'-UTR of the ZIKV genome. RESULTS: The ZIKV-5'-UTR assay exhibited the lowest in-vitro limit of detection (5-10 RNA copies/reaction and 3.0×10(-1) plaque-forming units/ml). Compared to the modified version of a widely adopted RT-PCR assay targeting the ZIKV-E gene, the ZIKV-5'-UTR assay showed better sensitivity in human clinical specimens, and representative mouse specimens, including many organs which are known to be involved in human ZIKV infection but difficult to obtain in clinical settings...
February 18, 2017: Tropical Medicine & International Health: TM & IH
https://www.readbyqxmd.com/read/28211687/isolation-and-structure-elucidation-of-glucosylated-colchicinoids-from-the-seeds-of-gloriosa-superba-by-lc-dad-spe-nmr
#2
Yancho Zarev, Kenn Foubert, Iliana Ionkova, Sandra Apers, Luc Pieters
Four new colchicinoids were isolated from the seeds of Gloriosa superba together with the known compounds colchicoside (4) and 3-de-O-methylcolchicine-3-O-β-d-glucopyranosyl-(1→4)-3-O-β-d-glucopyranoside (6), by means of conventional column chromatography and LC-DAD-SPE-NMR. The new compounds were identified as N-deacetyl-N-formyl-3-de-O-methylcolchicine-3-O-β-d-glucopyranoside (1), 3-de-O-methylcolchicine-3-O-β-d-glucopyranosyl-(1→6)-3-O-β-d-glucopyranoside (2), N-deacetyl-N-formyl-3-de-O-methylcolchicine-3-O-β-d-glucopyranosyl-(1→6)-3-O-β-d-glucopyranoside (3), and 3-de-O-methylcolchicine-3-O-β-d-glucopyranosyl-(1→3)-3-O-β-d-glucopyranoside (5)...
February 17, 2017: Journal of Natural Products
https://www.readbyqxmd.com/read/28202373/is-fatigue-associated-with-cognitive-dysfunction-in-early-parkinson-s-disease
#3
Benzi M Kluger, Kenn Freddy Pedersen, Ole-Bjorn Tysnes, Solgunn O Ongre, Bjørg Øygarden, Karen Herlofson
INTRODUCTION: Fatigue is a common and disabling symptom which may be seen in early Parkinson's disease (PD). Our understanding of the phenomenology and etiology of fatigue in PD is limited. The objective of this study was to determine whether fatigue was related to cognition in early PD patients. METHODS: The study is part of the Norwegian ParkWest project, a population-based cohort study, comprising 184 de novo, drug-naïve patients with PD. PD was diagnosed according to the Gelb criteria...
February 7, 2017: Parkinsonism & related Disorders
https://www.readbyqxmd.com/read/28185815/novel-antiviral-activity-and-mechanism-of-bromocriptine-as-a-zika-virus-ns2b-ns3-protease-inhibitor
#4
Jasper Fuk-Woo Chan, Kenn Ka-Heng Chik, Shuofeng Yuan, Cyril Chik-Yan Yip, Zheng Zhu, Kah-Meng Tee, Jessica Oi-Ling Tsang, Chris Chung-Sing Chan, Vincent Kwok-Man Poon, Gang Lu, Anna Jinxia Zhang, Kin-Kui Lai, Kwok-Hung Chan, Richard Yi-Tsun Kao, Kwok-Yung Yuen
Zika virus (ZIKV) infection is associated with congenital malformations in infected fetuses and severe neurological and other systemic complications in adults. There are currently limited anti-ZIKV treatment options that are readily available and safe for use in pregnancy. In this drug repurposing study, bromocriptine was found to have inhibitory effects on ZIKV replication in cytopathic effect inhibition, virus yield reduction, and plaque reduction assays. Time-of-drug-addition assay showed that bromocriptine exerted anti-ZIKV activity between 0 and 12 h post-ZIKV inoculation, corroborating with post-entry events in the virus replication cycle prior to budding...
February 6, 2017: Antiviral Research
https://www.readbyqxmd.com/read/28164379/peptide-nucleotide-antibiotic-microcin-c-is-a-potent-inducer-of-stringent-response-and-persistence-in-both-sensitive-and-producing-cells
#5
Julia Piskunova, Etienne Maisonneuve, Elsa Germain, Kenn Gerdes, Konstantin Severinov
Microcin C (McC) is a peptide-nucleotide antibiotic that inhibits aspartyl-tRNA synthetase. Here, we show that McC is a strong inducer of persistence in Escherichia coli. Persistence induced by McC is mediated by (p)ppGpp and requires chromosomally encoded toxin-antitoxin modules. McC-producing cells have increased persistence levels due to a combined effect of McC imported from the cultured medium and intracellularly synthesized antibiotic. McC-producing cells also induce persistence in sensitive cells during co-cultivation, underscoring complex interactions in bacterial communities where an antagonistic compound produced by one community member can benefit other members by increasing their ability to withstand antibiotics...
February 6, 2017: Molecular Microbiology
https://www.readbyqxmd.com/read/28160118/production-of-chemicals-from-c1-gases-co-co2-by-clostridium-carboxidivorans
#6
REVIEW
Ánxela Fernández-Naveira, Haris Nalakath Abubackar, María C Veiga, Christian Kennes
Bioprocesses in conventional second generation biorefineries are mainly based on the fermentation of sugars obtained from lignocellulosic biomass or agro-industrial wastes. An alternative to this process consists in gasifying those same feedstocks or even other carbon-containing materials to obtain syngas which can also be fermented by some anaerobic bacteria to produce chemicals or fuels. Carbon monoxide, carbon dioxide and hydrogen, which are the main components of syngas, are also found in some industrial waste gases, among others in steel industries...
March 2017: World Journal of Microbiology & Biotechnology
https://www.readbyqxmd.com/read/28152554/in-vitro-and-in-vivo-study-of-the-gastrointestinal-absorption-and-metabolisation-of-hymenocardine-a-cyclopeptide-alkaloid
#7
Emmy Tuenter, Sebastiaan Bijttebier, Kenn Foubert, Annelies Breynaert, Sandra Apers, Nina Hermans, Luc Pieters
Hymenocardine is a cyclopeptide alkaloid present in the root bark of Hymenocardia acida. In traditional African medicine, the leaves and roots of this plant are used to treat malaria, and moderate in vitro antiplasmodial activity has been reported for hymenocardine. However, in view of its peptide-like nature, potential metabolisation after oral ingestion has to be taken into account when considering in vivo experiments. In this study, the stability and small intestinal absorption of hymenocardine was assessed using an in vitro gastrointestinal dialysis model...
February 2, 2017: Planta Medica
https://www.readbyqxmd.com/read/28127922/excess-mortality-in-persons-with-severe-mental-disorders-a-multilevel-intervention-framework-and-priorities-for-clinical-practice-policy-and-research-agendas
#8
Nancy H Liu, Gail L Daumit, Tarun Dua, Ralph Aquila, Fiona Charlson, Pim Cuijpers, Benjamin Druss, Kenn Dudek, Melvyn Freeman, Chiyo Fujii, Wolfgang Gaebel, Ulrich Hegerl, Itzhak Levav, Thomas Munk Laursen, Hong Ma, Mario Maj, Maria Elena Medina-Mora, Merete Nordentoft, Dorairaj Prabhakaran, Karen Pratt, Martin Prince, Thara Rangaswamy, David Shiers, Ezra Susser, Graham Thornicroft, Kristian Wahlbeck, Abe Fekadu Wassie, Harvey Whiteford, Shekhar Saxena
Excess mortality in persons with severe mental disorders (SMD) is a major public health challenge that warrants action. The number and scope of truly tested interventions in this area remain limited, and strategies for implementation and scaling up of programmes with a strong evidence base are scarce. Furthermore, the majority of available interventions focus on a single or an otherwise limited number of risk factors. Here we present a multilevel model highlighting risk factors for excess mortality in persons with SMD at the individual, health system and socio-environmental levels...
February 2017: World Psychiatry: Official Journal of the World Psychiatric Association (WPA)
https://www.readbyqxmd.com/read/28117126/the-role-of-ct-scan-in-postoperative-evaluation-of-distal-radius-fractures-retrospective-analysis-in-regard-to-complications-and-revision-rates
#9
Michael G Jakubietz, Laura Mages, Robert K Zahn, Werner Kenn, Rafael G Jakubietz, Rainer H Meffert
BACKGROUND: Intraarticular fractures of the distal radius present a challenging problem for surgeons. While preoperative CT scans are considered helpful to understand the type of fracture and to choose an adequate approach, the role of postoperative CT scans is not yet clearly defined. The aim of this study was to analyze indications for postoperative CT scans and to evaluate its potential therapeutic consequence in regard to detection of complications and its influence on revision rates in intraarticular fractures...
January 20, 2017: Journal of Orthopaedic Science: Official Journal of the Japanese Orthopaedic Association
https://www.readbyqxmd.com/read/28108638/natural-course-of-mild-cognitive-impairment-in-parkinson-disease-a-5-year-population-based-study
#10
Kenn Freddy Pedersen, Jan Petter Larsen, Ole-Bjørn Tysnes, Guido Alves
OBJECTIVE: To examine the incidence, progression, and reversion of mild cognitive impairment in patients with Parkinson disease (PD-MCI) over 5 years. METHODS: A population-based cohort of patients with incident PD underwent repeated neuropsychological testing of attention, executive function, memory, and visuospatial abilities at baseline (n = 178), 1 year (n = 175), 3 years (n = 163), and 5 years (n = 150). Patients were classified as PD-MCI and diagnosed with dementia according to published criteria...
January 20, 2017: Neurology
https://www.readbyqxmd.com/read/28107443/identification-of-the-pla2g6-c-1579g-a-missense-mutation-in-papillon-dog-neuroaxonal-dystrophy-using-whole-exome-sequencing-analysis
#11
Masaya Tsuboi, Manabu Watanabe, Kazumi Nibe, Natsuko Yoshimi, Akihisa Kato, Masahiro Sakaguchi, Osamu Yamato, Miyuu Tanaka, Mitsuru Kuwamura, Kazuya Kushida, Takashi Ishikura, Tomoyuki Harada, James Kenn Chambers, Sumio Sugano, Kazuyuki Uchida, Hiroyuki Nakayama
Whole exome sequencing (WES) has become a common tool for identifying genetic causes of human inherited disorders, and it has also recently been applied to canine genome research. We conducted WES analysis of neuroaxonal dystrophy (NAD), a neurodegenerative disease that sporadically occurs worldwide in Papillon dogs. The disease is considered an autosomal recessive monogenic disease, which is histopathologically characterized by severe axonal swelling, known as "spheroids," throughout the nervous system. By sequencing all eleven DNA samples from one NAD-affected Papillon dog and her parents, two unrelated NAD-affected Papillon dogs, and six unaffected control Papillon dogs, we identified 10 candidate mutations...
2017: PloS One
https://www.readbyqxmd.com/read/28056080/comparison-of-ipaq-sf-and-two-other-physical-activity-questionnaires-with-accelerometer-in-adolescent-boys
#12
Triin Rääsk, Jarek Mäestu, Evelin Lätt, Jaak Jürimäe, Toivo Jürimäe, Uku Vainik, Kenn Konstabel
Self-report measures of physical activity (PA) are easy to use and popular but their reliability is often questioned. Therefore, the general aim of the present study was to investigate the association of PA questionnaires with accelerometer derived PA, in a sample of adolescent boys. In total, 191 pubertal boys (mean age 14.0 years) completed three self-report questionnaires and wore an accelerometer (ActiGraph GT1M) for 7 consecutive days. The PA questionnaires were: International Physical Activity Questionnaire-Short Form (IPAQ-SF), Tartu Physical Activity Questionnaire (TPAQ), and the Inactivity subscale from Domain-Specific Impulsivity (DSI) scale...
2017: PloS One
https://www.readbyqxmd.com/read/28049643/polyphosphate-nanoparticles-on-the-platelet-surface-trigger-contact-system-activation
#13
Johan J F Verhoef, Arjan D Barendrecht, Katrin F Nickel, Kim Dijkxhoorn, Ellinor Kenne, Linda Labberton, Owen J T McCarty, Raymond Schiffelers, Harry F Heijnen, Antoni P Hendrickx, Huub Schellekens, Marcel H Fens, Steven de Maat, Thomas Renné, Coen Maas
Polyphosphate is an inorganic polymer that can potentiate several interactions in the blood coagulation system. Blood platelets contain polyphosphate, and the secretion of platelet-derived polyphosphate has been associated with increased thrombus formation and activation of coagulation factor XII. However, the small polymer size of secreted platelet polyphosphate limits its capacity to activate factor XII in vitro. Thus, the mechanism by which platelet polyphosphate contributes to thrombus formation remains unclear...
January 3, 2017: Blood
https://www.readbyqxmd.com/read/28012898/development-of-a-high-throughput-multiplexed-real-time-rt-pcr-assay-for-detection-of-human-pegivirus-1-and-2
#14
Matthew Frankel, Kenn Forberg, Kelly E Coller, Michael G Berg, John Hackett, Gavin Cloherty, George J Dawson
Human Pegivirus 2 (HPgV-2) was recently identified in the bloodstream of HCV-infected and multiply transfused individuals. Initial reports show HPgV-2 circulates at a low prevalence in HCV co-infected individuals, necessitating testing of large cohorts of samples to identify infected persons. The identification of additional HPgV-2 cases was facilitated by the development of a high throughput and reliable molecular reverse transcription polymerase chain reaction (RT-PCR) assay intended for use on the automated Abbott m2000 system with a capability of extracting and testing 96 samples at once...
December 22, 2016: Journal of Virological Methods
https://www.readbyqxmd.com/read/28000003/long-term-risk-of-falls-in-an-incident-parkinson-s-disease-cohort-the-norwegian-parkwest-study
#15
Ylva Hivand Hiorth, Guido Alves, Jan Petter Larsen, Jörn Schulz, Ole-Bjørn Tysnes, Kenn Freddy Pedersen
The objective of this study is to examine the frequency, development, concomitants, and risk factors of falls in a population-based incident Parkinson's disease (PD) cohort. One hundred eighty-one drug-naïve patients with incident PD and 173 normal controls recruited from the Norwegian ParkWest study were prospectively monitored over 7 years. Information on falls was obtained biannually from patients, and at baseline and after 1, 3, 5, and 7 years of follow-up in control subjects. Generalized estimating equation models for correlated data were applied to investigate concomitant features of falls and risk factors for incident falls during 7 years of follow-up in PD...
December 20, 2016: Journal of Neurology
https://www.readbyqxmd.com/read/27987864/1d-nmr-methods-for-determination-of-degree-of-cross-linking-and-bdde-substitution-positions-in-ha-hydrogels
#16
Frida J Wende, Suresh Gohil, Lars I Nord, Anne Helander Kenne, Corine Sandström
Hyaluronic acid polymers cross-linked with BDDE are today among the most used hydrogels for biomedical applications. The physical properties of the hydrogels depend, among other parameters, on the degree of cross-linking of HA. Another parameter likely to affect the physical properties is the substitution position of the linker on the HA functional groups. A NMR-based method for the determination of these parameters in hyaluronic acid hydrogels is presented. The method is based on the degradation of HA cross linked hydrogels by chondroitinase ABC followed by one-dimensional (1)H and (13)C NMR analysis...
February 10, 2017: Carbohydrate Polymers
https://www.readbyqxmd.com/read/27984742/back-to-the-roots-deep-view-into-the-evolutionary-history-of-adp-ribosylation-opened-by-the-dna-targeting-toxin-antitoxin-module-dartg
#17
Alexander Harms, Kenn Gerdes
In this issue of Molecular Cell, Jankevicius et al. (2016) characterize the DarTG toxin-antitoxin module in which the DarT toxin ADP-ribosylates single-stranded DNA and the DarG antitoxin counteracts DarT by direct binding and by enzymatic removal of the ADP-ribosylation.
December 15, 2016: Molecular Cell
https://www.readbyqxmd.com/read/27980159/mechanisms-of-bacterial-persistence-during-stress-and-antibiotic-exposure
#18
REVIEW
Alexander Harms, Etienne Maisonneuve, Kenn Gerdes
Bacterial persister cells avoid antibiotic-induced death by entering a physiologically dormant state and are considered a major cause of antibiotic treatment failure and relapsing infections. Such dormant cells form stochastically, but also in response to environmental cues, by various pathways that are usually controlled by the second messenger (p)ppGpp. For example, toxin-antitoxin modules have been shown to play a major role in persister formation in many model systems. More generally, the diversity of molecular mechanisms driving persister formation is increasingly recognized as the cause of physiological heterogeneity that underlies collective multistress and multidrug tolerance of persister subpopulations...
December 16, 2016: Science
https://www.readbyqxmd.com/read/27940278/short-term-effects-of-supplemental-oxygen-on-6-minute-walk-test-outcomes-in-copd-patients-a-randomized-placebo-controlled-single-blind-cross-over-trial
#19
Inga Jarosch, Rainer Gloeckl, Eva Damm, Anna-Lena Schwedhelm, David Buhrow, Andreas Jerrentrup, Martijn A Spruit, Klaus Kenn
BACKGROUND: The acute effect of supplemental oxygen during exercise has been shown to differ largely among patients with COPD. It is unknown what the oxygen response is influenced by. METHODS: In a randomized and single-blinded fashion, 124 COPD patients underwent one 6-minute walk test on supplemental oxygen (6MWTO2) and one on compressed room air (6MWTRA) after a practice 6MWT. Both gases wedre delivered via standard nasal prongs (2 liters/min). For analyses, patients were stratified based on PaO2 values: (a) 34 patients with resting hypoxemia (HYX), (b) 43 patients with exercise-induced hypoxemia (EIH) and (c) 31 normoxemic patients (NOX) were compared...
December 8, 2016: Chest
https://www.readbyqxmd.com/read/27933893/isolation-and-structure-elucidation-by-lc-dad-ms-and-lc-dad-spe-nmr-of-cyclopeptide-alkaloids-from-the-roots-of-ziziphus-oxyphylla-and-evaluation-of-their-antiplasmodial-activity
#20
Emmy Tuenter, Rizwan Ahmad, Kenn Foubert, Adnan Amin, Maria Orfanoudaki, Paul Cos, Louis Maes, Sandra Apers, Luc Pieters, Vassiliki Exarchou
Nine cyclopeptide alkaloids (1-9), of which five (compounds 2, 3, 5, 8, and 9) are described herein for the first time, were isolated from roots of Ziziphus oxyphylla by means of conventional separation methods as well as semipreparative HPLC with DAD and ESIMS detection and LC-DAD-SPE-NMR. Structure elucidation was done by spectroscopic means. Nummularine-R (1), a previously known constituent from this species, was isolated along with its new derivatives O-desmethylnummularine-R (2) and O-desmethylnummularine-R N-oxide (3)...
November 23, 2016: Journal of Natural Products
keyword
keyword
83086
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"