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https://www.readbyqxmd.com/read/28339615/viable-offspring-after-imaging-of-ca2-oscillations-and-visualization-of-the-cortical-reaction-in-mouse-eggs%C3%A2
#1
Yuhkoh Satouh, Kaori Nozawa, Kazuo Yamagata, Takao Fujimoto, Masahito Ikawa
During mammalian fertilization, egg Ca 2+ oscillations are known to play pivotal roles in triggering downstream events such as resumption of the cell cycle and the establishment of blocks to polyspermy. However, viable offspring have not been obtained after monitoring Ca 2+ oscillations, and their spatiotemporal links to subsequent events are still to be examined. Therefore, the development of imaging methods to avoid phototoxic damage while labeling these events is required. Here, we examined the usefulness of genetically encoded Ca 2+ indicators for optical imaging (GECOs), in combination with spinning-disk confocal imaging...
February 3, 2017: Biology of Reproduction
https://www.readbyqxmd.com/read/28330174/salicornia-evaluating-the-halophytic-extremophile-as-a-food-and-a-pharmaceutical-candidate
#2
REVIEW
Seema Patel
Food insecurity is a major issue in current scenario where a large section of mankind is at risk of insufficient diet. As food productivity has its limits, the prospecting of unutilized or underutilized flora as food candidates is collectively recognized as a sustainable option. In the past decade, a number of obscure plants have been identified to be rich in dietary components and deemed fit for integration into the food platter. This review discusses a candidate Salicornia, belonging to family Amaranthaceae...
June 2016: 3 Biotech
https://www.readbyqxmd.com/read/28322147/molecular-characterization-and-antioxidant-potential-of-three-wild-culinary-medicinal-mushrooms-from-tripura-northeast-india
#3
Aparajita Roy Das, Madhusmita Borthakur, Ajay Krishna Saha, Santa Ram Joshi, Panna Das
The aim of this study was to characterize 3 wild culinary-medicinal mushrooms using molecular tools and to analyze their antioxidant activity. Antioxidant properties were studied by evaluating free radical scavenging, reducing power, and chelating effect. The mushrooms were identified as Lentinus squarrosulus, L. tuber-regium, and Macrocybe gigantean by amplifying internal transcribed spacer regions of ribosomal DNA. The results demonstrated that the methanolic extract of M. gigantean has the highest free radical scavenging effect and chelating effect, whereas the methanolic extract of L...
2017: International Journal of Medicinal Mushrooms
https://www.readbyqxmd.com/read/28322143/in-vitro-and-in-vivo-antidiabetic-evaluation-of-selected-culinary-medicinal-mushrooms-agaricomycetes
#4
Varinder Singh, Gurleen Kaur Bedi, Richa Shri
Management of type 2 diabetes by delaying or preventing glucose absorption using natural products is gaining significant attention. Edible mushrooms are well documented for their nutritional and medicinal properties. This investigation was designed to evaluate the antidiabetic activity of aqueous extracts of selected culinary-medicinal mushrooms, namely, Pleurotus ostreatus, Calocybe indica, and Volvariella volvacea, using in vitro models (α-amylase inhibition assay, glucose uptake by yeast cells, and glucose adsorption capacity)...
2017: International Journal of Medicinal Mushrooms
https://www.readbyqxmd.com/read/28320235/methanol-extract-of-nigella-sativa-seed-induces-changes-in-the-levels-of-neurotransmitter-amino-acids-in-male-rat-brain-regions
#5
Tarek El-Naggar, María Emilia Carretero, Carmen Arce, María Pilar Gómez-Serranillos
CONTEXT: Nigella sativa L. (Ranunculaceae) (NS) has been used for medicinal and culinary purposes. Different parts of the plant are used to treat many disorders. OBJECTIVE: This study investigates the effects of NS methanol extract on brain neurotransmitter amino acid levels. MATERIALS AND METHODS: We measured the changes in aspartate, glutamate, glycine and γ-aminobutyric acid in five brain regions of male Wistar rats after methanol extract treatment...
December 2017: Pharmaceutical Biology
https://www.readbyqxmd.com/read/28303042/effect-of-dehulling-germination-and-cooking-on-nutrients-anti-nutrients-fatty-acid-composition-and-antioxidant-properties-in-lentil-lens-culinaris
#6
R S Pal, Anuradha Bhartiya, Pradhuman Yadav, Lakshmi Kant, K K Mishra, J P Aditya, A Pattanayak
The changes in chemical composition, antioxidant activity and fatty acid composition of lentil flour after dehulling, germination and cooking of seeds were investigated. Dehulling showed no significant effect on protein content, however, protein content decreased in most of the varieties after germination and cooking. Total soluble sugars (TSS) content increased significantly after dehulling (2.0-41.64 %) and cooking (2.08-31.07 %) whereas, germination had no significant effect on TSS content. Total lipids increased significantly after dehulling (21...
March 2017: Journal of Food Science and Technology
https://www.readbyqxmd.com/read/28299619/double-and-triple-positive-tumor-markers-predict-early-recurrence-and-poor-survival-in-patients-with-hepatocellular-carcinoma-within-the-milan-criteria-and-child-pugh-class-a
#7
Tomoki Ryu, Yuko Takami, Yoshiyuki Wada, Masaki Tateishi, Hajime Matsushima, Kazuhiro Mikagi, Hideki Saitsu
The prognostic implications of the expression patterns of three tumor markers, alpha-fetoprotein (AFP), the Lens culinaris agglutinin-reactive fraction of AFP (AFP-L3) and des-γ-carboxy prothrombin (DCP), have been evaluated in patients with hepatocellular carcinoma (HCC). From January 1994 to December 2014, 1182 consecutive patients underwent hepatic resection and surgical microwave ablation for HCC at our institution. This study analyzed 475 patients within the Milan criteria and Child-Pugh class A. Cumulative overall survival (OS) and disease-free survival (DFS) rates were analyzed relative to the number of positive tumor markers...
March 15, 2017: Journal of Gastrointestinal Surgery: Official Journal of the Society for Surgery of the Alimentary Tract
https://www.readbyqxmd.com/read/28296720/usefulness-of-afp-afp-l3-and-pivka-ii-and-their-combinations-in-diagnosing-hepatocellular-carcinoma
#8
Sang Joon Park, Jae Young Jang, Soung Won Jeong, Young Kyu Cho, Sae Hwan Lee, Sang Gyune Kim, Sang-Woo Cha, Young Seok Kim, Young Deok Cho, Hong Soo Kim, Boo Sung Kim, Suyeon Park, Hae In Bang
Alpha-fetoprotein (AFP), Lens culinaris-agglutinin-reactive fraction of AFP (AFP-L3), and protein induced by vitamin K absence or antagonist-II (PIVKA-II) are widely used as tumor markers for the diagnosis of hepatocellular carcinoma (HCC). This study compared the diagnostic values of AFP, AFP-L3, and PIVKA-II individually and in combination to find the best biomarker or biomarker panel.Seventy-nine patients with newly diagnosed HCC and 77 non-HCC control patients with liver cirrhosis were enrolled. AFP, AFP-L3, and PIVKA-II were measured in the same serum samples using microchip capillary electrophoresis and a liquid-phase binding assay on an automatic analyzer...
March 2017: Medicine (Baltimore)
https://www.readbyqxmd.com/read/28267567/safety-assessment-of-mushrooms-in-dietary-supplements-by-combining-analytical-data-with-in-silico-toxicology-evaluation
#9
Karen M VanderMolen, Jason G Little, Vincent P Sica, Tamam El-Elimat, Huzefa A Raja, Nicholas H Oberlies, Timothy R Baker, Catherine Mahony
Despite growing popularity in dietary supplements, many medicinal mushrooms have not been evaluated for their safe human consumption using modern techniques. The multifaceted approach described here relies on five key principles to evaluate the safety of non-culinary fungi for human use: (1) identification by sequencing the nuclear ribosomal internal transcribed spacer (ITS) region (commonly referred to as ITS barcoding), (2) screening an extract of each fungal raw material against a database of known fungal metabolites, (3) comparison of these extracts to those prepared from grocery store-bought culinary mushrooms using UHPLCPDA-ELS-HRMS, (4) review of the toxicological and chemical literature for each fungus, and (5) evaluation of data establishing presence in-market...
March 4, 2017: Food and Chemical Toxicology
https://www.readbyqxmd.com/read/28266846/ligand-binding-properties-of-the-lentil-lipid-transfer-protein-molecular-insight-into-the-possible-mechanism-of-lipid-uptake
#10
Zakhar O Shenkarev, Daria N Melnikova, Ekaterina I Finkina, Stanislav V Sukhanov, Ivan A Boldyrev, Albina K Gizatullina, Konstantin S Mineev, Alexander Sergeevich Arseniev, Tatiana V Ovchinnikova
The lentil lipid transfer protein, designated as Lc-LTP2, was isolated from the Lens culinaris seeds. The protein belongs to LTP1 subfamily and consists of 93 amino acid residues. Its spatial structure contains four α-helices (H1-H4) and a long C-terminal tail. Here, we report the ligand-binding properties of Lc-LTP2. The fluorescent TNS binding assay revealed that the Lc-LTP2 affinity for saturated and unsaturated fatty acids was enhanced with a decrease in acyl chain length. Measurements of boundary potential in planar lipid bilayers and calcein dye-leakage in vesicular systems revealed preferential interaction of Lc-LTP2 with the negatively charged membranes...
March 7, 2017: Biochemistry
https://www.readbyqxmd.com/read/28265493/transcriptome-analysis-of-polygonum-minus-reveals-candidate-genes-involved-in-important-secondary-metabolic-pathways-of-phenylpropanoids-and-flavonoids
#11
Kok-Keong Loke, Reyhaneh Rahnamaie-Tajadod, Chean-Chean Yeoh, Hoe-Han Goh, Zeti-Azura Mohamed-Hussein, Zamri Zainal, Ismanizan Ismail, Normah Mohd Noor
BACKGROUND: Polygonum minus is an herbal plant in the Polygonaceae family which is rich in ethnomedicinal plants. The chemical composition and characteristic pungent fragrance of Polygonum minus have been extensively studied due to its culinary and medicinal properties. There are only a few transcriptome sequences available for species from this important family of medicinal plants. The limited genetic information from the public expressed sequences tag (EST) library hinders further study on molecular mechanisms underlying secondary metabolite production...
2017: PeerJ
https://www.readbyqxmd.com/read/28264475/association-between-dietary-share-of-ultra-processed-foods-and-urinary-concentrations-of-phytoestrogens-in-the-us
#12
Eurídice Martínez Steele, Carlos A Monteiro
The aim of this study was to examine the relationship between dietary contribution of ultra-processed foods and urinary phytoestrogen concentrations in the US. Participants from cross-sectional 2009-2010 National Health and Nutrition Examination Survey aged 6+ years, selected to measure urinary phytoestrogens and with one 24-h dietary recall were evaluated (2692 participants). Food items were classified according to NOVA (a name, not an acronym), a four-group food classification based on the extent and purpose of industrial food processing...
February 28, 2017: Nutrients
https://www.readbyqxmd.com/read/28255312/radioprotective-effects-of-lentil-sprouts-against-x-ray-radiation
#13
Abbas Haghparast, Kamran Mansouri, Samane Moradi, Fatemeh Dadashi, Saeed Eliasi, Mahdi Sobhani, Kambiz Varmira
The present study investigated the radioprotective efficacy of lentil (Lens culinaris) sprouts against X-ray radiation-induced cellular damage. Lentil seeds were dark germinated at low temperature and the sprout extract was prepared in PBS. Free radical scavenging of extract was evaluated using 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay and then the radioprotective potency of extract (0 to 1000 μg/mL) on the lymphocyte cells was determined by lactate dehydrogenases assay. Moreover, micronuclei assay was assessed using the cytokinesis-block technique...
February 2017: Research in Pharmaceutical Sciences
https://www.readbyqxmd.com/read/28245170/crude-flavonoid-extract-of-medicinal-herb-zingibar-officinal-inhibits-proliferation-and-induces-apoptosis-in-hepatocellular-carcinoma-cells
#14
Osama A Abu-Zinadah, Ayman I Elkady, Rania Abd El Hamid Hussein
There is an urgent need to improve the clinical management of hepatocellular carcinoma (HCC), one of the most common causes of global cancer-related deaths. Zingibar officinale is a medicinal herb used throughout history for both culinary and medicinal purposes. It has antioxidant, anticarcinogenic, and free radical scavenging properties. Previously, we proved that the crude flavonoid extract of Z. officinale (CFEZO) inhibited growth and induced apoptosis in several cancer cell lines. However, the effect of the CFEZO on an HCC cell line has not yet been evaluated...
December 21, 2016: Oncology Research
https://www.readbyqxmd.com/read/28241862/transcriptome-analysis-of-lentil-lens-culinaris-medikus-in-response-to-seedling-drought-stress
#15
Dharmendra Singh, Chandan Kumar Singh, Jyoti Taunk, Ram Sewak Singh Tomar, Ashish Kumar Chaturvedi, Kishor Gaikwad, Madan Pal
BACKGROUND: Drought stress is one of the most harmful abiotic stresses in crop plants. As a moderately drought tolerant crop, lentil is a major crop in rainfed areas and a suitable candidate for drought stress tolerance research work. Screening for drought tolerance stress under hydroponic conditions at seedling stage with air exposure is an efficient technique to select genotypes with contrasting traits. Transcriptome analysis provides valuable resources, especially for lentil, as here the information on complete genome sequence is not available...
February 27, 2017: BMC Genomics
https://www.readbyqxmd.com/read/28236404/petroleum-ether-sub-fraction-of-rosemary-extract-improves-hyperlipidemia-and-insulin-resistance-by-inhibiting-srebps
#16
Zhi-Shen Xie, Ling-Jun Zhong, Xiao-Meng Wan, Meng-Ning Li, Hua Yang, Ping Li, Xiao-Jun Xu
As a culinary and medicinal herb, rosemary is widely used. The present work aimed to investigate the effects of rosemary extracts on metabolic diseases and the underlying mechanisms of action. Liver cells stably expressing SREBP reporter were used to evaluate the inhibitory effects of different fractions of rosemary extracts on SREBP activity. The obese mice induced by Western-type diet were orally administered with rosemary extracts or vehicle for 7 weeks, the plasma and tissue lipids were analyzed. SREBPs and their target genes were measured by quantitative RT-PCR...
October 2016: Chinese Journal of Natural Medicines
https://www.readbyqxmd.com/read/28235614/anti-inflammatory-activity-of-the-essential-oil-obtained-from-ocimum-basilicum-complexed-with-%C3%AE-cyclodextrin-%C3%AE-cd-in-mice
#17
Lindaiane Bezerra Rodrigues, Anita Oliveira Brito Pereira Bezerra Martins, Jaime Ribeiro-Filho, Francisco Rafael Alves Santana Cesário, Fyama Ferreira E Castro, Thaís Rodrigues de Albuquerque, Maria Neyze Martins Fernandes, Bruno Anderson Fernandes da Silva, Lucindo José Quintans Júnior, Adriano Antunes de Sousa Araújo, Paula Dos Passos Menezes, Paula Santos Nunes, Isabella Gonçalves Matos, Henrique Douglas Melo Coutinho, Almir Goncalves Wanderley, Irwin Rose Alencar de Menezes
Cyclodextrins (CDs) are cyclic oligosaccharides can enhance the bioavailability of drugs. Ocimum basilicum is an aromatic plant found in Brazil used in culinary. The essential oil of this plant presents anti-edematogenic and anti-inflammatory activities in acute and chronic inflammation. The aim of this study was to investigate the anti-inflammatory effects of the essential oil obtained from O. basilicum complexed with β - cyclodextrin (OBEO/β-CD) in mice. The complexation with β-cyclodextrin (β-CD) was performed by different methods and analyzed by differential scanning calorimetry (DSC), thermogravimetry (TG) and scanning electron microscopy (SEM)...
February 22, 2017: Food and Chemical Toxicology
https://www.readbyqxmd.com/read/28231138/essential-oils-in-foods-from-ancient-times-to-the-21st-century
#18
EDITORIAL
Esther Sendra
Medicinal plants and culinary herbs have been used since ancient times. Essential oils (EO) are a mixture of numerous compounds, mainly terpenes, alcohols, acids, esters, epoxides, aldehydes, ketones,aminesandsulfides,thatareprobablyproducedbyplantsasaresponsetostress[1].[...].
June 14, 2016: Foods (Basel, Switzerland)
https://www.readbyqxmd.com/read/28231130/evaluation-of-turmeric-powder-adulterated-with-metanil-yellow-using-ft-raman-and-ft-ir-spectroscopy
#19
Sagar Dhakal, Kuanglin Chao, Walter Schmidt, Jianwei Qin, Moon Kim, Diane Chan
Turmeric powder (Curcuma longa L.) is valued both for its medicinal properties and for its popular culinary use, such as being a component in curry powder. Due to its high demand in international trade, turmeric powder has been subject to economically driven, hazardous chemical adulteration. This study utilized Fourier Transform-Raman (FT-Raman) and Fourier Transform-Infra Red (FT-IR) spectroscopy as separate but complementary methods for detecting metanil yellow adulteration of turmeric powder. Sample mixtures of turmeric powder and metanil yellow were prepared at concentrations of 30%, 25%, 20%, 15%, 10%, 5%, 1%, and 0...
May 17, 2016: Foods (Basel, Switzerland)
https://www.readbyqxmd.com/read/28230238/integrating-straw-yield-and-quality-into-multi-dimensional-improvement-of-lentil-lens-culinaris
#20
Ashraf Alkhtib, J Wamatu, T Tolemariam Ejeta, B Rischkowsky
BACKGROUND: Lentil straw is an important source of fodder for livestock in Africa, South Asia and the Middle East. However, improvement programs of lentil do not pay attention to straw traits, neither are straw traits considered in release criteria of new varieties. This study aimed to determine whether straw traits can be integrated into multi-trait improvement of lentil. RESULTS: Wide genotypic variation (P<0.001) was found in grain yield, straw yield and nutritive value of straw...
February 23, 2017: Journal of the Science of Food and Agriculture
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