Francisco Chagas Filho, Janyerson Dannys Pereira Silva, Caio Petri, João Santos Lima Almendra, Ícaro Araújo de Sousa, Suzana Maria Galvão Cavalcanti, Bagnólia A Silva, Margareth Fátima Formiga Melo, Paulo Marques da Silva Cavalcanti
γ-Aminobutyric acid (GABA) is the main inhibitory neurotransmitter in adult central nervous system (CNS) synapses, but it excites immature CNS neurons as well as neurons in the myenteric plexus. The present work aimed to determine whether GABA-induced nonadrenergic, noncholinergic (NANC) neuronal-mediated relaxation of the rat duodenum is dependent on the activity of Na+ K+ Cl- cotransporters (NKCC) and requires calcium influx. In the presence of guanethidine (3 µmol/L), atropine (3 µmol/L), and indomethacin (1 µmol/L), relaxations induced by GABA (100 µmol/L), KCl (5-10 mmol/L) and electrical field stimulation (1-8 Hz, 2 ms, 60 V), but not those induced by bradykinin (10-100 nmol/L) were abolished by lidocaine (300 µmol/L)...
July 26, 2022: Canadian Journal of Physiology and Pharmacology