Andrew J Kueh, Maria I Bergamasco, Anna Quaglieri, Belinda Phipson, Connie S N Li-Wai-Suen, Ingrid M Lönnstedt, Yifang Hu, Zhi-Ping Feng, Chris Woodruff, Rose E May, Stephen Wilcox, Alexandra L Garnham, Michael P Snyder, Gordon K Smyth, Terence P Speed, Tim Thomas, Anne K Voss
In the conventional model of transcriptional activation, transcription factors bind to response elements and recruit co-factors, including histone acetyltransferases. Contrary to this model, we show that the histone acetyltransferase KAT7 (HBO1/MYST2) is required genome wide for histone H3 lysine 14 acetylation (H3K14ac). Examining neural stem cells, we find that KAT7 and H3K14ac are present not only at transcribed genes but also at inactive genes, intergenic regions, and in heterochromatin. KAT7 and H3K14ac were not required for the continued transcription of genes that were actively transcribed at the time of loss of KAT7 but indispensable for the activation of repressed genes...
January 13, 2023: Cell Reports