Read by QxMD icon Read

Microdissection testis

M C Lardone, F Argandoña, M Flórez, A Parada-Bustamante, M Ebensperger, C Palma, A Piottante, A Castro
Several observational studies have showed a combination of lower testosterone (T) to LH ratio and higher estradiol (E2 ) to T ratio in secretory infertile men compared to men with normal spermatogenesis, suggesting a steroidogenic dysfunction of Leydig cells (Lc) that may involve increased aromatase activity. Low T/LH ratio is associated with Lc hyperplasia, which together with LH hyperstimulation may represent compensation for impaired T production. Aromatase expression and oestrogen production are mainly detected in Lc of the testis, although Sertoli and germ cells also contribute to testicular aromatase activity...
January 2017: Andrology
Britta Klein, Thomas Haggeney, Daniela Fietz, Sivanjah Indumathy, Kate L Loveland, Mark Hedger, Sabine Kliesch, Wolfgang Weidner, Martin Bergmann, Hans-Christian Schuppe
STUDY QUESTION: Which immune cells and cytokine profiles are characteristic for testicular germ cell neoplasia and what consequences does this have for the understanding of the related testicular immunopathology? SUMMARY ANSWER: The unique immune environment of testicular germ cell neoplasia comprises B cells and dendritic cells as well as high transcript levels of IL-6 and other B cell supporting or T helper cell type 1 (Th1)-driven cytokines and thus differs profoundly from normal testis or inflammatory lesions associated with hypospermatogenesis...
October 2016: Human Reproduction
Qiong Yu, Xiuli Gu, Xuejun Shang, Honggang Li, Chengliang Xiong
Cell-free seminal mRNA (cfs-mRNA) contains testis-specific transcripts from bilateral testes. This study determined the presence of DEAD box polypeptide 4 (DDX4) in cfs-mRNA to identify and characterize the incidence of Sertoli cell-only (SCO) syndrome in men with non-obstructive azoospermia (NOA). DDX4 cfs-mRNA was determined in 315 men with NOA, and compared with testicular samples obtained by microdissection from 19 NOA patients. Karyotype and azoospermia factor microdeletion analysis were performed, and clinical features were evaluated...
August 2016: Reproductive Biomedicine Online
Craig Niederberger
No abstract text is available yet for this article.
May 2015: Journal of Urology
Aaron M Bernie, Ranjith Ramasamy, Peter N Schlegel
Azoospermia in men requires microsurgical reconstruction or a procedure for sperm retrieval with assisted reproduction to allow fertility. While the chance of successful retrieval of sperm in men with obstructive azoospermia approaches >90%, the chances of sperm retrieval in men with non-obstructive azoospermia (NOA) are not as high. Conventional procedures such as fine needle aspiration of the testis, testicular biopsy and testicular sperm extraction are successful in 20-45% of men with NOA. With microdissection testicular sperm extraction (micro-TESE), the chance of successful retrieval can be up to 60%...
2013: Basic and Clinical Andrology
Yasuharu Nishimura, Yusuke Tomita, Akira Yuno, Yoshihiro Yoshitake, Masanori Shinohara
Recent genome-wide cDNA microarray analysis of gene expression profiles in comprehensive tumor types coupled with isolation of cancer tissues by laser-microbeam microdissection have revealed ideal tumor-associated antigens (TAAs) that are frequently overexpressed in various cancers including head and neck squamous cell cancer (HNSCC) and lung cancer, but not in most normal tissues except for testis, placenta, and fetal organs. Preclinical studies using HLA-transgenic mice and human T cells in vitro showed that TAA-derived CTL-epitope short peptides (SPs) are highly immunogenic and induce HLA-A2 or -A24-restricted CTLs...
May 2015: Cancer Science
Joanna Noelke, Joachim Wistuba, Oliver S Damm, Daniela Fietz, Jonathan Gerber, Marion Gaehle, Ralph Brehm
The Sertoli cell (SC)-specific knockout (KO) of connexin43 (Cx43) results in spermatogenic arrest at the level of spermatogonia and/or SC-only syndrome. Histology of the interstitial compartment suggests Leydig cell (LC) hyperplasia. Our aim has been to investigate possible effects of the SC-specific KO of Cx43 (SCCx43KO) on interstitial LC. We therefore counted LC via the optical dissector method (per microliter of testicular tissue and per testis) and found LC to be significantly increased in SCCx43KO(-/-) compared with wild-type mice...
August 2015: Cell and Tissue Research
T Aydin, M Sofikerim, B Yucel, M Karadag, F Tokat
Non-obstructive azoospermia (NOA) is characterised by absence of sperm in the ejaculate. Significant relationship between the pattern of the testis histopathology of NOA and successful sperm retrieval rate is well known. In this study, we assess efficacy of testicular histopathology on sperm retrieval rates and intracytoplasmic sperm injection results after microdissection testicular sperm extraction in cases of non-obstructive azoospermia. It is a retrospective analysis of 111 NOA patients who have histopathological confirmation...
2015: Journal of Obstetrics and Gynaecology: the Journal of the Institute of Obstetrics and Gynaecology
Meric Karacan, Murat Ulug, Ayse Arvas, Ziya Cebi, Serdar Erkan, Teksen Camlıbel
OBJECTIVE: To determine live birth rate via m-TESE and ICSI in men who had a previous conventional testicular biopsy. STUDY DESIGN: Retrospective study was conducted to analyze 86 m-TESE procedures for ICSI in NOA patients who had a previous conventional TESE. Only motile spermatozoa were used for ICSI and all other forms were discarded. Women under the age of 42 years and who produced at least 3 oocytes in response to controlled ovarian stimulation were included in the study...
December 2014: European Journal of Obstetrics, Gynecology, and Reproductive Biology
Boback M Berookhim, Gianpiero D Palermo, Nikica Zaninovic, Zev Rosenwaks, Peter N Schlegel
OBJECTIVE: To study the outcomes of microdissection testicular sperm extraction (microTESE) among men with pure Sertoli cell-only histology on diagnostic testicular biopsy. DESIGN: Retrospective cohort study. SETTING: Tertiary referral center. PATIENT(S): Six hundred forty patients with pure Sertoli cell-only histology on testicular biopsy who underwent microTESE by a single surgeon. INTERVENTION(S): MicroTESE...
November 2014: Fertility and Sterility
Jas S Kalsi, Paras Shah, Yau Thum, Asif Muneer, David J Ralph, Suks Minhas
OBJECTIVE: To assess the outcome of micro-dissection testicular exploration sperm extraction (m-TESE) as a salvage treatment in men with non-obstructive azoospermia (NOA) in whom no sperm was previously found on single/multiple TESE or testicular sperm aspiration (TESA). PATIENTS AND METHODS: In all, 58 men with NOA underwent m-TESE. All the patients had previously undergone either single/multiple TESE or TESA with no sperm found. All the patients underwent an m-TESE using a standard technique...
September 2015: BJU International
Mehmet Erol Yildirim, Akif Koc, Ikbal Cekmen Kaygusuz, Hüseyin Badem, Omer Faruk Karatas, Ersin Cimentepe, Dogan Unal
PURPOSE: To evaluate the predictive power of luteinizing hormone (LH), follicle-stimulating hormone (FSH), testosterone, testicular biopsy histology and male age were evaluated with respect to the success of sperm retrieval in a microdissection testicular sperm extraction (microTESE) procedure, pregnancy and live birth rates. MATERIALS AND METHODS: We examined the data of 131 infertile men with non-obstructive azoospermia, who have undergone microTESE operation...
July 2014: Urology Journal
Marta Melis, Giacomo Diaz, David E Kleiner, Fausto Zamboni, Juraj Kabat, Jinping Lai, Giulia Mogavero, Ashley Tice, Ronald E Engle, Steven Becker, Charles R Brown, Jeffrey C Hanson, Jaime Rodriguez-Canales, Michael Emmert-Buck, Sugantha Govindarajan, Michael Kew, Patrizia Farci
BACKGROUND: The molecular mechanisms whereby hepatitis B virus (HBV) induces hepatocellular carcinoma (HCC) remain elusive. We used genomic and molecular techniques to investigate host-virus interactions by studying multiple areas of the same liver from patients with HCC. METHODS: We compared the gene signature of whole liver tissue (WLT) versus laser capture-microdissected (LCM) hepatocytes along with the intrahepatic expression of HBV. Gene expression profiling was performed on up to 17 WLT specimens obtained at various distances from the tumor center from individual livers of 11 patients with HCC and on selected LCM samples...
2014: Journal of Translational Medicine
Matthew Wosnitzer, Marc Goldstein, Matthew P Hardy
Azoospermia is classified as obstructive azoospermia (OA) or non-obstructive azoospermia (NOA), each having very different etiologies and treatments. The etiology, diagnosis, and management of azoospermia were reviewed and relevant literature summarized. Differentiation between these two etiologies is of paramount importance and is contingent upon thorough history and physical examination and indicated laboratory/genetic testing. OA occurs secondary to obstruction of the male reproductive tract, and is diagnosed through a combination of history/physical examination, laboratory testing, genetics (CFTR for congenital OA), and imaging studies...
2014: Spermatogenesis
Natasha R Catlin, Susan M Huse, Kim Boekelheide
Over the past decade, laser capture microdissection (LCM) has grown as a tool for gene expression profiling of small numbers of cells from tumor samples and of specific cell populations in complex tissues. LCM can be used to study toxicant effects on selected cell populations within the testis at different stages of spermatogenesis. There are several LCM-related hurdles to overcome, including issues inherent to the method itself, as well as biases that result from amplifying the LCM-isolated RNA. Many technical issues associated with the LCM method are addressed here, including increasing RNA yield and obtaining more accurate quantification of RNA yields...
December 2014: Toxicologic Pathology
Daniela Fietz, Clara Ratzenböck, Katja Hartmann, Oksana Raabe, Sabine Kliesch, Wolfgang Weidner, Jörg Klug, Martin Bergmann
Estrogen signaling is considered to play an important role in spermatogenesis, spermiogenesis and male fertility. Estrogens can act via the two nuclear estrogen receptors ESR1 (ERα) and ESR2 (ERβ) or via the intracellular G-protein-coupled estrogen receptor 1 (GPER, formerly GPR30). Several reports on the localization and expression of all three receptors in the human testis have been published but are controversial particularly in case of ERα. Contrary to previous studies, we decided therefore to evaluate expression of all three receptors in the testis by a number of different methods and in comparison with MCF-7 cells...
October 2014: Histochemistry and Cell Biology
Natasha R Catlin, Susan M Huse, Kim Boekelheide
Testicular effects of chemical mixtures may differ from those of the individual chemical constituents. This study assessed the co-exposure effects of the model germ cell- and Sertoli cell-specific toxicants, X-irradiation (x-ray), and 2,5-hexanedione (HD), respectively. In high-dose studies, HD has been shown to attenuate x-ray-induced germ cell apoptosis. Adult rats were exposed to different levels of x-ray (0.5 Gy, 1 Gy, and 2 Gy) or HD (0.33%), either alone or in combination. To assess cell type-specific attenuation of x-ray effects with HD co-exposure, we used laser capture microdissection (LCM) to enrich the targeted cell population and examine a panel of apoptosis-related transcripts using PCR arrays...
December 2014: Toxicologic Pathology
Nicholas Haddad, Khalid Al-Rabeeah, Ronald Onerheim, Armand Zini
OBJECTIVE: To report a case of an infertile man with nonobstructive azoospermia who underwent simultaneous radical orchiectomy for testicular cancer and testicular sperm extraction (TESE) for preservation of fertility. DESIGN: Case report and literature review. SETTING: University teaching hospital. PATIENT(S): A couple being treated for infertility. INTERVENTION(S): Radical orchiectomy with simultaneous TESE...
April 2014: Fertility and Sterility
Ranjith Ramasamy, Nikunj N Trivedi, Jennifer E Reifsnyder, Gianpiero D Palermo, Zev Rosenwaks, Peter N Schlegel
OBJECTIVE: To evaluate the effect of male age on the outcome of microdissection testicular sperm extraction (micro-TESE) and assisted reproductive technology. DESIGN: Clinical retrospective study. SETTING: Center for reproductive medicine at a tertiary university hospital. PATIENT(S): One thousand sixty-seven men with nonobstructive azoospermia. INTERVENTION(S): Micro-TESE, with intracytoplasmic sperm injection when sperm found...
March 2014: Fertility and Sterility
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"