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Soon Auck Hong, Su Hyun Yoo, Jene Choi, Stanley J Robboy, Kyu-Rae Kim
CONTEXT: - Papillary immature metaplasia (PIM) is a known papillary cervical lesion associated with low-risk human papilloma virus (LR-HPV). OBJECTIVE: - To evaluate additional clinicopathologic features and the HPV genotypes of PIM and discuss the presumptive cell of origin. DESIGN: - A total of 26 PIM cases were evaluated by p16INK4a , cytokeratin (CK) 7, and CK17 immunohistochemical stainings. Human papilloma virus genotyping was performed, by using HPV DNA Chip, HPV polymerase chain reaction (PCR), and real-time PCR...
April 13, 2018: Archives of Pathology & Laboratory Medicine
Magnus Aassved Hjort, Håkon Hov, Pegah Abdollahi, Esten Nymoen Vandsemb, Unn-Merete Fagerli, Bendik Lund, Tobias Schmidt Slørdahl, Magne Børset, Torstein Baade Rø
Background: Phosphatase of regenerating liver-3 (PRL-3) is implicated in oncogenesis of hematological and solid cancers. PRL-3 expression increases metastatic potential, invasiveness and is associated with poor prognosis. With this study, we aimed to show a possible oncogenic role of PRL-3 in classical Hodgkin lymphoma (cHL). Methods: PRL-3 expression was measured in 25 cHL patients by immunohistochemistry and gene expression was analyzed from microdissected malignant cells...
2018: Experimental Hematology & Oncology
Enrico Tiacci, Erik Ladewig, Gianluca Schiavoni, Alex Penson, Elisabetta Fortini, Valentina Pettirossi, Yuchun Wang, Ariele Rosseto, Alessandra Venanzi, Sofija Vlasevska, Roberta Pacini, Simonetta Piattoni, Alessia Tabarrini, Alessandra Pucciarini, Barbara Bigerna, Alessia Santi, Alessandro M Gianni, Simonetta Viviani, Antonello Cabras, Stefano Ascani, Barbara Crescenzi, Cristina Mecucci, Laura Pasqualucci, Raul Rabadan, Brunangelo Falini
Dissecting the pathogenesis of classical Hodgkin lymphoma (cHL), a common cancer in young adults, remains challenging due to the rarity of tumor cells in involved tissues (usually <5%). Here, we analyzed the coding genome of cHL by microdissecting tumor and normal cells from 34 patient biopsies, for a total of ~50,000 singly isolated lymphoma cells. We uncovered several recurrently mutated genes, namely STAT6 (32% of cases), GNA13 (24%), XPO1 (18%) and ITPKB (16%), and document the functional role of mutant STAT6 in sustaining tumor cell viability...
April 12, 2018: Blood
Li Gong, Yanhong Li, Xinyi Li, Qian Tu, Xiaorong Mou, Shumei Wang, Yi Wan, Qiang Lu, Juzheng Wang, Wendong Zhang, Shaojun Zhu, Xiujuan Han, Li Yao, Jiarui Zhang, Gaosheng Huang, Wei Zhang
OBJECTIVE: To investigate the association between human parvovirus B19 (B19V) infection in the thymus and myasthenia gravis (MG). METHODS: The presence of human B19V DNA and protein was assessed in 138 samples, including 68 thymic hyperplasia (39 with MG), 58 thymoma (23 with MG), and 12 normal thymus tissues, by nested polymerase chain reaction, immunohistochemistry, laser capture microdissection, and sequencing in a double-blinded manner. RESULTS: B19V DNA was mainly detected in thymic hyperplasia, and the positive rate (41...
April 9, 2018: Clinical Microbiology and Infection
Hsin-Hsiung Chen, Li-Kai Tsai, Kuan-Yu Liao, Tung-Chien Wu, Yun-Hsin Huang, Yuan-Chun Huang, Szu-Wei Chang, Pei-Yu Wang, Yeou-Ping Tsao, Show-Li Chen
BACKGROUND: Nuclear receptor interaction protein (NRIP) is a calcium/calmodulin (CaM) binding protein. Nuclear receptor interaction protein interacts with CaM to activate calcineurin and CaMKII signalling. The conventional NRIP knockout mice (global knockout) showed muscular abnormality with reduction of muscle oxidative functions and motor function defects. METHODS: To investigate the role of NRIP on neuromuscular system, we generated muscle-restricted NRIP knockout mice [conditional knockout (cKO)]...
April 2, 2018: Journal of Cachexia, Sarcopenia and Muscle
John R Lozada, Thais Basili, Fresia Pareja, Barbara Alemar, Arnaud C Paula, Rodrigo Gularte-Merida, Dilip D Giri, Patrizia Querzoli, Gabor Cserni, Emad A Rakha, Maria Pia Foschini, Jorge S Reis-Filho, Edi Brogi, Britta Weigelt, Felipe C Geyer
AIMS: Solid papillary breast carcinoma resembling the tall cell variant of papillary thyroid neoplasms (BPTC), also known as solid papillary carcinoma with reverse polarity, is a rare histologic type of breast cancer that morphologically resembles the tall cell variant of papillary thyroid carcinoma. BPTCs are characterized by IDH2 R172 hotspot somatic mutations or mutually exclusive TET2 somatic mutations, concurrently with mutations affecting PI3K pathway-related genes. We sought to characterize their histology, and investigate the frequency of IDH2 and PIK3CA mutations in an independent cohort of BPTCs, as well as in conventional solid papillary carcinomas (SPCs)...
March 30, 2018: Histopathology
Rauni Klami, Harri Mankonen, Antti Perheentupa
Non-obstructive azoospermia (NOA) is the most severe form of male infertility, defined by lack of spermatozoa in the ejaculate caused by impaired spermatogenesis. The chance of biological fatherhood of these men has been improved since the introduction of microdissection testicular sperm extraction (MD-TESE) combined with intracytoplasmic sperm injection. A thorough patient evaluation preoperatively is essential to recognize any underlying conditions, and to assist in patient counseling on the sperm recovery rate and pregnancy results...
March 27, 2018: Reproductive Biology
Masako Ishikawa, Kentaro Nakayama, Kohei Nakamura, Ruriko Ono, Kaori Sanuki, Hitomi Yamashita, Tomoka Ishibashi, Toshiko Minamoto, Kouji Iida, Sultana Razia, Noriyoshi Ishikawa, Satoru Kyo
Aim: Endometriosis-related ovarian neoplasms (ERONs) have recently attracted considerable attention; however, the prevalence and patterns of ARID1A and POLE mutations in ERONs have not been studied in detail. The aim of this study was to investigate not only the carcinogenesis of ERONs, but also the prognostic significance of several gene mutations in this cohort. We used DNA purified from only tumor epithelial cells, from which fibroblasts were removed, using a specific method we called "liquid microdissection"...
March 13, 2018: Oncotarget
Beth Frazer, Hugh Kearney, Jane Cryan, Alan Beausang, Francesca Brett, Michael A Farrell, Patrick G Buckley
The presence of oligodendroglioma-like areas in pilocytic astrocytoma may give rise to pathologic diagnostic uncertainty. This study aims to determine if the oligodendroglioma-like areas present in some pilocytic astrocytomas (PA) possess the signature 1p/19q codeletion that is characteristic of classical oligodendroglioma. Array comparative genomic hybridization was carried out on 12 PA samples, from which oligodendroglioma-like areas were microdissected and used as the template DNA source. 1p/19q codeletions were not found in any of the oligodendroglioma areas in PAs...
March 29, 2018: Clinical Neuropathology
Ying Xu, Weixing Wu, Yunxia Fan, Shuyi Jiang, Xiaoyu Jia, Wenhui Su
BACKGROUND/AIMS: Transforming growth factor-β3 (TGF-β3) has been proved to perturb the blood-testis barrier (BTB) by accelerating junction protein endocytosis in Sertoli cells (SCs) to accommodate the traversing of preleptotene spermatocytes across the BTB around stage VIII in rat. Yet the molecular network underlying the impairment of TGF-β3 on BTB integrity is not fully elucidated. Our study herein was designed to investigate the participation of microRNA-142-3p (miR-142-3p), which has been reported to affect TGF-β3 signaling via different pathways, during BTB dynamics and the corresponding mechanisms...
March 22, 2018: Cellular Physiology and Biochemistry
James K Liu
We present a case of a giant Rathke's cleft cyst with significant superior extension into the third and right lateral ventricles and lateral extension into the left Sylvian fissure and over the anterior clinoid process. An extended modification of the frontotemporal approach was performed using a combined frontotemporal transbasal approach. This allowed wide exposure to both transsylvian and subfrontal corridors to the retrochiasmatic space. This video atlas demonstrates the operative technique and surgical nuances of the skull base approach, microdissection of the tumor, and safe handling of the neurovascular structures...
April 2018: Journal of Neurological Surgery. Part B, Skull Base
James K Liu, Jean Anderson Eloy
We present a pediatric case of a retrochiasmatic craniopharyngioma in the suprasellar region with third ventricular extension that was resected through a purely endoscopic endonasal approach (EEA) via the transplanum transtuberculum corridor. The patient is a 12-year-old boy who presented with progressive visual loss and panhypopituitarism. The EEA allows direct visualization of the undersurface of the optic chiasm and hypothalamus so that safe and meticulous tumor dissection can be performed to preserve these critical neurovascular structures...
April 2018: Journal of Neurological Surgery. Part B, Skull Base
Wenting Jiang, Han Wang, Yongmei Cui, Yiyan Lei, Yuefeng Wang, Di Xu, Neng Jiang, Yangshan Chen, Yu Sun, Yang Zhang, Jessica Cao, Zunfu Ke
Background: Circulating tumor cells (CTCs) detection, an approach considered to be "liquid biopsy", is a potential alternative method in clinical use for early diagnosis of solid tumor progression. Methods: In this study, we developed a poly (lactic- co -glycolic acid) (PLGA) - nanofiber (PN)-NanoVelcro chip as an efficient device for simple and rapid capture of CTCs from peripheral blood. We evaluated the device performance by assessing the capture efficiency and purity...
2018: International Journal of Nanomedicine
Florian Schlotter, Arda Halu, Shinji Goto, Mark C Blaser, Simon C Body, Lang H Lee, Hideyuki Higashi, Daniel M DeLaughter, Joshua D Hutcheson, Payal Vyas, Tan Pham, Maximillian A Rogers, Amitabh Sharma, Christine E Seidman, Joseph Loscalzo, Jonathan G Seidman, Masanori Aikawa, Sasha A Singh, Elena Aikawa
Background -No pharmacological therapy exists for calcific aortic valve disease (CAVD), which confers a dismal prognosis without invasive valve replacement. The search for therapeutics and early diagnostics is challenging since CAVD presents in multiple pathological stages. Moreover, it occurs in the context of a complex, multi-layered tissue architecture, a rich and abundant extracellular matrix phenotype, and a unique, highly plastic and multipotent resident cell population. Methods -A total of 25 human stenotic aortic valves obtained from valve replacement surgeries were analyzed by multiple modalities, including transcriptomics and global unlabeled and label-based tandem-mass-tagged proteomics...
March 27, 2018: Circulation
Gleb N Artemov, Mikhail I Gordeev, Alina A Kokhanenko, Anton V Moskaev, Alena I Velichevskaya, Vladimir N Stegniy, Igor V Sharakhov, Maria V Sharakhova
BACKGROUND: Anopheles beklemishevi is a member of the Maculipennis group of malaria mosquitoes that has the most northern distribution among other members of the group. Although a cytogenetic map for the larval salivary gland chromosomes of this species has been developed, a high-quality standard cytogenetic photomap that enables genomics and population genetics studies of this mosquito at the adult stage is still lacking. METHODS: In this study, a cytogenetic map for the polytene chromosomes of An...
March 27, 2018: Parasites & Vectors
Ana Gordon, Shravan Kumar Kannan, Karine Gousset
Microproteomic studies have improved our knowledge of cell biology. Yet, with MS analysis, accuracy can be lost for protein identification and quantification when using heterogeneous samples. Laser capture microdissection (LCM) allows for the enrichment of specific subsets of cells to study their proteome, however, sample fixation is necessary. Unfortunately, fixation hampers MS results due to protein crosslinking. The aim of this study was to identify both a fixation protocol and an extraction method that returns the best yield of proteins for downstream MS analysis, while preserving cellular structures...
March 26, 2018: Proteomics
Aparna B Baxi, Camille Lombard-Banek, Sally A Moody, Peter Nemes
The molecular program by which embryonic ectoderm is induced to form neural tissue is essential to understanding normal and impaired development of the central nervous system. Xenopus has been a powerful vertebrate model in which to elucidate this process. However, abundant vitellogenin (yolk) proteins in cells of the early Xenopus embryo interfere with protein detection by high-resolution mass spectrometry (HRMS), the technology of choice for identifying these gene products. Here, we systematically evaluated strategies of bottom-up proteomics to enhance proteomic detection from the neural ectoderm (NE) of X...
March 26, 2018: ACS Chemical Neuroscience
Raphael Röthlisberger, Valerie Aurore, Susanne Boemke, Hannes Bangerter, Mathias Bergmann, George N Thalmann, Valentin Djonov
INTRODUCTION: The aim of this study was to investigate the nerve supply to the structures in the male lesser pelvis and review its clinical relevance, especially during nerve sparing surgery. MATERIAL AND METHODS: Three formalin-embalmed and 16 Thiel-embalmed male hemipelves were used. They were microdissected after repeated treatments with nitric acid diluted 1:10 with milliQ-water. RESULTS: The inferior hypogastric plexus is a fan-ike structure lateral from the rectum on the fascia of the levator ani...
March 25, 2018: Clinical Anatomy
Xavier Raffoux, Mickael Bourge, Fabrice Dumas, Olivier C Martin, Matthieu Falque
Allelic recombination due to meiotic crossovers is a major driver of genome evolution, as well as a key player for the selection of high-performing genotypes in economically important species. Therefore, we developed a high throughput and low cost method to measure recombination rates and crossover patterning (including interference) in large populations of the budding yeast S. cerevisiae. Recombination and interference were analyzed by flow cytometry, which allows escaping time-consuming steps such as tetrad microdissection or spore growth...
March 25, 2018: Yeast
Ning Wang, Zi-Han Liu, Hong Zou, Li-Juan Pang, Wen-Yi Gu, Jian-Ming Hu, Dong-Mei Li, Jin Zhao, Jun Zhang, Chun-Xia Liu, Wen-Jie Zhang, Yan Qi, Feng Li
Synovial sarcoma (SS) is a mesenchymal malignant neoplasm showing characteristics of epithelial-mesenchymal biphasic differentiation. SS is of uncertain cellular origin; however, studies have suggested that SS originates from a somatic stem cell population.In this study, we aimed to determine whether differential morphological features of the epithelial-mesenchymal transition (EMT) contributed to the tumorigenesis of SS invasion and metastasis. Twelve paraffin-embedded formalin-fixed tissue (FFPE) SS tissue specimens were obtained, and laser capture microdissection (LCM) with the ArcturusXT system and small chip method (SCM) were used to isolate and purify spindle and epithelial cells from SS specimens...
March 25, 2018: Clinical and Experimental Pharmacology & Physiology
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