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https://www.readbyqxmd.com/read/29446696/expression-profiles-of-stress-related-genes-in-islets-from-donors-with-progressively-impaired-glucose-metabolism
#1
Marcus Lundberg, Anton Stenwall, Angie Tegehall, Olle Korsgren, Oskar Skog
It is currently unknown how the islet transcriptional pattern changes as glucose metabolism deteriorates and progresses to fulminant type 2 diabetes (T2D). In this study, we hypothesized that islets from donors with elevated HbA1c levels, but not yet diagnosed with T2D, would show signs of cell stress on a transcriptional level. Laser capture microdissection and qPCR arrays including 330 genes related to mitochondria, oxidative stress, or the unfolded protein response were used to extract and analyze islets from organ donors with HbA1c <5...
February 15, 2018: Islets
https://www.readbyqxmd.com/read/29445151/the-vesicle-trafficking-regulator-pn_scd1-is-demethylated-and-overexpressed-in-florets-of-apomictic-paspalum-notatum-genotypes
#2
Marika Bocchini, Giulio Galla, Fulvio Pupilli, Michele Bellucci, Gianni Barcaccia, Juan Pablo A Ortiz, Silvina C Pessino, Emidio Albertini
Apomixis (asexual reproduction through seeds) is considered a deviation of the sexual reproductive pathway leading to the development of clonal progenies genetically identical to the mother plant. Here we used the Methylation-Sensitive Amplification Polymorphism (MSAP) technique to characterize cytosine methylation patterns occurring in florets of sexual and aposporous Paspalum notatum genotypes, in order to identify epigenetically-controlled genes putatively involved in apomixis development. From twelve polymorphic MSAP-derived sequences, one (PN_6...
February 14, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29440233/comprehensive-characterisation-of-compartment-specific-long-non-coding-rnas-associated-with-pancreatic-ductal-adenocarcinoma
#3
Luis Arnes, Zhaoqi Liu, Jiguang Wang, Hans Carlo Maurer, Irina Sagalovskiy, Marta Sanchez-Martin, Nikhil Bommakanti, Diana C Garofalo, Dina A Balderes, Lori Sussel, Kenneth P Olive, Raul Rabadan
OBJECTIVE: Pancreatic ductal adenocarcinoma (PDA) is a highly metastatic disease with limited therapeutic options. Genome and transcriptome analyses have identified signalling pathways and cancer driver genes with implications in patient stratification and targeted therapy. However, these analyses were performed in bulk samples and focused on coding genes, which represent a small fraction of the genome. DESIGN: We developed a computational framework to reconstruct the non-coding transcriptome from cross-sectional RNA-Seq, integrating somatic copy number alterations (SCNA), common germline variants associated to PDA risk and clinical outcome...
February 10, 2018: Gut
https://www.readbyqxmd.com/read/29438521/leydig-cell-dysfunction-is-associated-with-post-transcriptional-deregulation-of-cyp17a1-in-men-with-sertoli-cell-only-syndrome
#4
M C Lardone, F Argandoña, M Lorca, A Piottante, M Flórez, C Palma, M Ebensperger, A Castro
STUDY QUESTION: Is the expression of steroidogenic enzyme 17α-Hydroxylase/17,20-Lyase (CYP17A1) downregulated in Leydig cells (LCs) of men with spermatogenic failure and compensated impairment of LC function i.e. a low testosterone to LH (T/LH) ratio? SUMMARY ANSWER: Although the transcriptional expression of CYP17A1 is increased, its protein expression is decreased, in isolated LCs of men with spermatogenic failure and reduced serum T/LH. WHAT IS KNOWN ALREADY: Primary spermatogenic defects have been associated with functional and morphological abnormalities of LCs, characterized by decreased serum testosterone (T) levels, decreased T/LH, increased 17β-estradiol (E2) and E2/T ratio, and larger clusters of LCs (LC hyperplasia)...
February 9, 2018: Molecular Human Reproduction
https://www.readbyqxmd.com/read/29438174/presence-or-absence-of-significant-hpve4-expression-in-high-grade-anal-intraepithelial-neoplasia-with-p16-ki-67-positivity-indicates-distinct-patterns-of-neoplasia-a-study-combining-immunohistochemistry-and-laser-capture-microdissection-pcr
#5
Annemiek Leeman, Edyta C Pirog, John Doorbar, Miekel M van de Sandt, Folkert J van Kemenade, David Jenkins, Wim G V Quint
Progression of anal intraepithelial neoplasia (AIN) involves transition from productive to transforming human papillomavirus (HPV) infection. Grading aims to distinguish productive low-grade AIN from high-grade anal intraepithelial neoplasia (HGAIN) with risk of cancer. We describe immunohistochemical patterns in AIN adding a novel marker for initiation of the productive phase of the HPV life cycle (panHPVE4) to those for cell cycle activity (Ki-67) and transforming activity of HPVE7 gene (p16). We studied 67 anal biopsies for suspected anal neoplasia (17 normal, 15 AIN1, 20 AIN2, 15 AIN3) from 54 men who have sex with men at New York Presbyterian Hospital, USA...
February 12, 2018: American Journal of Surgical Pathology
https://www.readbyqxmd.com/read/29435937/laser-capture-microdissection-of-epithelium-from-a%C3%A2-wound-healing-model-for-microrna-analysis
#6
Alyne Simões, Zujian Chen, Yan Zhao, Lin Chen, Virgilia Macias, Luisa A DiPietro, Xiaofeng Zhou
MicroRNAs are ~22 nucleotide-long noncoding RNAs influencing many cellular processes (including wound healing) by their regulatory functions on gene expression. The ability to analyze microRNA in different cells at the wound site is essential for understanding the critical role(s) of microRNA during various phases of wound healing. Laser capture micro-dissection (LCM) is an effective method to distinguish between relevant and non-relevant cells or tissues and enables the researcher to obtain homogeneous, ultra-pure samples from heterogeneous starting material...
2018: Methods in Molecular Biology
https://www.readbyqxmd.com/read/29435921/isolation-of-viral-infected-brain-regions-for-mirna-profiling-from-formalin-fixed-paraffin-embedded-tissues-by-laser-capture-microdissection
#7
Anna Majer, Stephanie A Booth
Brain is a highly heterogeneous organ with numerous layers of specialized cells. Viral infection further adds complexity to downstream analysis because only a subpopulation of the brain is infected. In these instances, molecular changes that occur within infected cells are not truly reflected when whole tissue is used for downstream analysis. Laser capture microdissection (LCM) is a tool that allows for the selection and isolation of cells or regions of interest as determined by microscopic observation. It provides a platform for visually selecting the tissue that truly represents the material one wishes to study, such as viral infected cells...
2018: Methods in Molecular Biology
https://www.readbyqxmd.com/read/29434944/microrna-196a-5p-is-a-potential-prognostic-marker-of-delayed-lymph-node-metastasis-in-early-stage-tongue-squamous-cell-carcinoma
#8
Tessho Maruyama, Kazuhide Nishihara, Masato Umikawa, Akira Arasaki, Toshiyuki Nakasone, Fumikazu Nimura, Akira Matayoshi, Kimiko Takei, Saori Nakachi, Ken-Ichi Kariya, Naoki Yoshimi
MicroRNAs (miRs) are expected to serve as prognostic tools for cancer. However, many miRs have been reported as prognostic markers of recurrence or metastasis in oral squamous cell carcinoma patients. We aimed to determine the prognostic markers in early-stage tongue squamous cell carcinoma (TSCC). Based on previous studies, we hypothesized that miR-10a, 10b, 196a-5p, 196a-3p, and 196b were prognostic markers and we retrospectively performed miR expression analyses using formalin-fixed paraffin-embedded sections of surgical specimens...
February 2018: Oncology Letters
https://www.readbyqxmd.com/read/29434872/regulator-of-g-protein-signaling-5-enhances-portal-vein-invasion-in-hepatocellular-carcinoma
#9
Yumi Umeno, Sachiko Ogasawara, Jun Akiba, Satoshi Hattori, Hironori Kusano, Osamu Nakashima, Hironori Koga, Takuji Torimura, Ryoji Yamakawa, Hirohisa Yano
Portal vein invasion (PVI) is a major prognostic factor in hepatocellular carcinoma (HCC). The aim of the present study was to identify molecules that regulate PVI. Sections of cancerous tissue, paired noncancerous tissue and the PVI area were collected from 3 frozen HCC sections, using laser microdissection. The present study focused on 3 upregulated molecules, integrin β3 (ITGB3), secreted phosphoprotein 1 (SPP1) and regulator of G-protein signaling 5 (RGS5), and 2 molecules that were downregulated in PVI tissue compared with cancer tissue, metallothionein 1G (MT1G) and metallothionein 1H (MT1H), as determined by cDNA microarray analysis...
February 2018: Oncology Letters
https://www.readbyqxmd.com/read/29434651/combining-laser-assisted-microdissection-lam-and-rna-seq-allows-to-perform-a-comprehensive-transcriptomic-analysis-of-epidermal-cells-of-arabidopsis-embryo
#10
Kaori Sakai, Ludivine Taconnat, Nero Borrega, Jennifer Yansouni, Véronique Brunaud, Christine Paysant-Le Roux, Etienne Delannoy, Marie-Laure Martin Magniette, Loïc Lepiniec, Jean Denis Faure, Sandrine Balzergue, Bertrand Dubreucq
Background: Genome-wide characterization of tissue- or cell-specific gene expression is a recurrent bottleneck in biology. We have developed a sensitive approach based on ultra-low RNA sequencing coupled to laser assisted microdissection for analyzing different tissues of the small Arabidopsis embryo. Methods and results: We first characterized the number of genes detected according to the quantity of tissue yield and total RNA extracted. Our results revealed that as low as 0...
2018: Plant Methods
https://www.readbyqxmd.com/read/29432451/microexon-gene-transcriptional-profiles-and-evolution-provide-insights-into-blood-processing-by-the-schistosoma-japonicum-esophagus
#11
Xiao-Hong Li, Ricardo DeMarco, Leandro X Neves, Sally R James, Katherine Newling, Peter D Ashton, Jian-Ping Cao, R Alan Wilson, William Castro-Borges
BACKGROUND: Adult schistosomes have a well-developed alimentary tract comprising an oral sucker around the mouth, a short esophagus and a blind ending gut. The esophagus is not simply a muscular tube for conducting blood from the mouth to gut but is divided into compartments, surrounded by anterior and posterior glands, where processing of ingested blood is initiated. Self-cure of rhesus macaques from a Schistosoma japonicum infection appears to operate by blocking the secretory functions of these glands so that the worms cease feeding and slowly starve to death...
February 12, 2018: PLoS Neglected Tropical Diseases
https://www.readbyqxmd.com/read/29427253/lack-of-connexin-40-decreases-the-calcium-sensitivity-of-renin-secreting-juxtaglomerular-cells
#12
Dominik Steppan, Lisa Geis, Lin Pan, Kenneth Gross, Charlotte Wagner, Armin Kurtz
The so-called calcium paradoxon of renin describes the phenomenon that exocytosis of renin from juxtaglomerular cells of the kidney is stimulated by lowering of the extracellular calcium concentration. The yet poorly understood effect of extracellular calcium on renin secretion appears to depend on the function of the gap junction protein connexin 40 (Cx40) in renin-producing cells. This study aimed to elucidate the role of Cx40 for the calcium dependency of renin secretion in more detail by investigating if Cx40 function is really essential for the influence of extracellular calcium on renin secretion, if and how Cx40 affects intracellular calcium dynamics in renin-secreting cells and if Cx40-mediated gap junctional coupling of renin-secreting cells with the mesangial cell area is relevant for the influence of extracellular calcium on renin secretion...
February 9, 2018: Pflügers Archiv: European Journal of Physiology
https://www.readbyqxmd.com/read/29413225/microdissection-testicular-sperm-extraction-micro-tese-predictive-value-of-preoperative-hormonal-levels-and-pathology-in-non-obstructive-azoospermia
#13
Alper Eken, Feray Gulec
The aim of this study was to evaluate the predictive value of preoperative hormonal levels and pathology, as well as the outcome of microsurgical testicular sperm extraction in patients with non-obstructive azoospermia (NOA), presenting to our clinic for treatment of infertility. The records of 145 men with NOA who underwent microdissection testicular sperm extraction (micro-TESE) between March 2013 and November 2016 were studied. The patient's age, testicular volume, hormonal profile for follicle-stimulating hormone (FSH), luteinizing hormone (LH), and testosterone (TT), and testicular pathology were recorded...
February 2018: Kaohsiung Journal of Medical Sciences
https://www.readbyqxmd.com/read/29412457/prospective-patient-stratification-into-robust-cancer-cell-intrinsic-subtypes-from-colorectal-cancer-biopsies
#14
Matthew Alderdice, Susan D Richman, Simon Gollins, Peter Stewart, Chris Hurt, Richard Adams, Amy McCorry, Aideen Roddy, Dale Vimalachandran, Claudio Isella, Enzo Medico, Tim Maughan, Darragh G McArt, Mark Lawler, Philip D Dunne
Colorectal cancer (CRC) biopsies underpin accurate diagnosis, but are also relevant for patient stratification in molecularly-guided clinical trials. The consensus molecular subtypes (CMS) and colorectal cancer intrinsic subtypes (CRIS) transcriptional signatures have potential clinical utility for improving prognostic/predictive patient assignment. However, their ability to provide robust classification, particularly in pre-treatment biopsies from multiple regions or at different time points remains untested...
February 7, 2018: Journal of Pathology
https://www.readbyqxmd.com/read/29401583/argininosuccinate-synthetase-1-contributes-to-gastric-cancer-invasion-and-progression-by-modulating-autophagy
#15
Chung-Ying Tsai, Hsiang-Cheng Chi, Lang-Ming Chi, Huang-Yu Yang, Ming-Ming Tsai, Kam-Fai Lee, Hsiang-Wei Huang, Li-Fang Chou, Ann-Joy Cheng, Chih-Wei Yang, Chia-Siu Wang, Kwang-Huei Lin
Argininosuccinate synthetase 1 (ASS1) is a rate-limited enzyme in arginine biosynthesis. The oncogenic potential of ASS1 in terms of prognosis and cancer metastasis in arginine prototrophic gastric cancer (GC) remains unclear at present. We identify differentially expressed proteins in microdissected GC tumor cells relative to adjacent nontumor epithelia by isobaric mass tag for relative and absolute quantitation proteomics analysis. GC cells with stable expression or depletion of ASS1 were further analyzed to identify downstream molecules...
January 10, 2018: FASEB Journal: Official Publication of the Federation of American Societies for Experimental Biology
https://www.readbyqxmd.com/read/29396107/oligomeric-amyloid-%C3%AE-preferentially-targets-neuronal-and-not-glial-mitochondrial-encoded-mrnas
#16
Diego Mastroeni, Jennifer Nolz, Omar M Khdour, Shobana Sekar, Elaine Delvaux, Lori Cuyugan, Winnie S Liang, Sidney M Hecht, Paul D Coleman
INTRODUCTION: Our laboratories have demonstrated that accumulation of oligomeric amyloid β (OAβ) in neurons is an essential step leading to OAβ-mediated mitochondrial dysfunction. METHODS: Alzheimer's disease (AD) and matching control hippocampal neurons, astrocytes, and microglia were isolated by laser-captured microdissection from the same subjects, followed by whole-transcriptome sequencing. Complementary in vitro work was performed in OAβ-treated differentiated SH-SY5Y, followed by the use of a novel CoQ10 analogue for protection...
January 20, 2018: Alzheimer's & Dementia: the Journal of the Alzheimer's Association
https://www.readbyqxmd.com/read/29395098/distinctive-molecular-signature-and-activated-signaling-pathways-in-aortic-smooth-muscle-cells-of-patients-with-myocardial-infarction
#17
Thidathip Wongsurawat, Chin Cheng Woo, Antonis Giannakakis, Xiao Yun Lin, Esther Sok Hwee Cheow, Chuen Neng Lee, Mark Richards, Siu Kwan Sze, Intawat Nookaew, Vladimir A Kuznetsov, Vitaly Sorokin
BACKGROUND AND AIMS: We aim to identify significant transcriptome alterations of vascular smooth muscle cells (VSMCs) in the aortic wall of myocardial infarction (MI) patients. Providing a robust transcriptomic signature, we aim to highlight the most likely aberrant pathway(s) in MI VSMCs. METHODS AND RESULTS: Laser-captured microdissection (LCM) was used to obtain VSMCs from aortic wall tissues harvested during coronary artery bypass surgery. Microarray gene analysis was applied to analyse VSMCs from 17 MI and 19 non-MI patients...
January 29, 2018: Atherosclerosis
https://www.readbyqxmd.com/read/29392864/an-efficient-method-for-eccrine-gland-isolation-from-human-scalp
#18
F Jimenez, M Alam, I Hernandez, E Poblet, J A Hardman, R Paus
We describe a simple and efficient method to isolate eccrine sweat glands from the human scalp. This method is inspired by the hair graft harvesting method used in hair transplantation. Based on the recently described anatomical relationship between the scalp hair follicle and the eccrine gland, we have found that scalp follicular unit grafts are an excellent eccrine gland isolation source, especially for the coiled component. In order to make the gland visible for stereoscopic microdissection, the follicular units need to be previously stained with a vital dye like Methylene Blue or Neutral Red...
February 2, 2018: Experimental Dermatology
https://www.readbyqxmd.com/read/29392703/probing-glioblastoma-tissue-heterogeneity-with-laser-capture-microdissection
#19
Jean-Pierre Gagner, David Zagzag
Among various methods now available to isolate distinct cell populations or even single cells for DNA/RNA and proteomic analysis, laser capture microdissection (LCM) offers a unique opportunity to study cells in their topological contexts. This chapter focuses on the preparation of LCM membrane slides, tissue staining and laser microdissection of cells of interest from frozen or formalin-fixed, paraffin-embedded glioblastoma tissue.
2018: Methods in Molecular Biology
https://www.readbyqxmd.com/read/29390136/mir-374-5p-mir-379-5p-and-mir-503-5p-regulate-proliferation-and-hypertrophic-differentiation-of-growth-plate-chondrocytes-in-male-rats
#20
Youn Hee Jee, Jinhee Wang, Shanna Yue, Melissa Jennings, Samuel J H Clokie, Ola Nilsson, Julian Lui, Jeffrey Baron
Growth plate chondrocytes undergo sequential differentiation to form the resting (RZ), proliferative (PZ), and hypertrophic zones (HZ). The important role of microRNAs (miRNAs) in the growth plate was previously revealed by cartilage-specific ablation of Dicer, an enzyme essential for biogenesis of many miRNAs. To identify specific miRNAs that regulate differentiation of PZ chondrocytes to HZ chondrocytes, we microdissected individual growth plate zones from juvenile rats and performed miRNA profiling using a solution hybridization method and also miRNA-seq...
January 30, 2018: Endocrinology
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