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https://www.readbyqxmd.com/read/28721719/imprinted-nanovelcro-microchips-for-isolation-and-characterization-of-circulating-fetal-trophoblasts-toward-noninvasive-prenatal-diagnostics
#1
Shuang Hou, Jie-Fu Chen, Min Song, Yazhen Zhu, Yu Jen Jan, Szu Hao Chen, Tzu-Hua Weng, Dean-An Ling, Shang-Fu Chen, Tracy Ro, An-Jou Liang, Tom Lee, Helen Jin, Man Li, Lian Liu, Yu-Sheng Hsiao, Peilin Chen, Hsiao-Hua Yu, Ming-Song Tsai, Margareta D Pisarska, Angela Chen, Li-Ching Chen, Hsian-Rong Tseng
Circulating fetal nucleated cells (CFNCs) in maternal blood offer an ideal source of fetal genomic DNA for noninvasive prenatal diagnostics (NIPD). We developed a class of nanoVelcro microchips to effectively enrich a subcategory of CFNCs, i.e., circulating trophoblasts (cTBs) from maternal blood, which can then be isolated with single-cell resolution by a laser capture microdissection (LCM) technique for downstream genetic testing. We first established a nanoimprinting fabrication process to prepare the LCM-compatible nanoVelcro substrates...
July 19, 2017: ACS Nano
https://www.readbyqxmd.com/read/28716282/serum-amyloid-p-component-a-novel-potential-player-in-vessel-degeneration-in-cadasil
#2
Akihito Nagatoshi, Mitsuharu Ueda, Akihiko Ueda, Masayoshi Tasaki, Yasuteru Inoue, Yihong Ma, Teruaki Masuda, Mayumi Mizukami, Sayaka Matsumoto, Takayuki Kosaka, Takayuki Kawano, Takaaki Ito, Yukio Ando
In cerebral autosomal dominant arteriopathy with subcortical infarcts and leukoencephalopathy (CADASIL), granular osmiophilic material (GOM) may play some roles in inducing cerebrovascular events. To elucidate the pathogenesis of CADASIL, we used laser microdissection and liquid chromatography-tandem mass spectrometry to analyze cerebrovascular lesions of patients with CADASIL for GOM. The analyses detected serum amyloid P component (SAP), annexin A2, and periostin as the proteins with the largest increase in the samples, which also demonstrated NOTCH3...
August 15, 2017: Journal of the Neurological Sciences
https://www.readbyqxmd.com/read/28708506/expression-and-localization-of-cyclooxygenases-in-the-oviduct-of-laying-hens-during-the-ovulatory-cycle
#3
M Elhamouly, N Isobe, Y Yoshimura
Prostaglandins (PGs) play important roles in regulation of the functions of the hen oviduct. However, little is known about the expression and localization of the rate-limiting cyclooxygenases (COX-1 and COX-2) in the oviduct. The aim of this study was to determine the COXs expression and localization in the different segments of the oviduct and to investigate changes in their expression levels during the ovulatory cycle of laying hens. White Leghorn laying hens were killed at 0, 4, 7, 16 and 24 h after oviposition, and samples from the infundibulum, magnum, isthmus, uterus, and vagina were collected...
June 13, 2017: Theriogenology
https://www.readbyqxmd.com/read/28702092/pat-h-ms-coupled-with-laser-microdissection-to-study-histone-post-translational-modifications-in-selected-cell-populations-from-pathology-samples
#4
Roberta Noberini, Rémi Longuespée, Cristina Richichi, Giancarlo Pruneri, Mark Kriegsmann, Giuliana Pelicci, Tiziana Bonaldi
BACKGROUND: Aberrations in histone post-translational modifications (hPTMs) have been linked with various pathologies, including cancer, and could not only represent useful biomarkers but also suggest possible targetable epigenetic mechanisms. We have recently developed an approach, termed pathology tissue analysis of histones by mass spectrometry (PAT-H-MS), that allows performing a comprehensive and quantitative analysis of histone PTMs from formalin-fixed paraffin-embedded pathology samples...
2017: Clinical Epigenetics
https://www.readbyqxmd.com/read/28700589/ank1-is-up-regulated-in-laser-captured-microglia-in-alzheimer-s-brain-the-importance-of-addressing-cellular-heterogeneity
#5
Diego Mastroeni, Shobana Sekar, Jennifer Nolz, Elaine Delvaux, Katie Lunnon, Jonathan Mill, Winnie S Liang, Paul D Coleman
Recent epigenetic association studies have identified a new gene, ANK1, in the pathogenesis of Alzheimer's disease (AD). Although strong associations were observed, brain homogenates were used to generate the data, introducing complications because of the range of cell types analyzed. In order to address the issue of cellular heterogeneity in homogenate samples we isolated microglial, astrocytes and neurons by laser capture microdissection from CA1 of hippocampus in the same individuals with a clinical and pathological diagnosis of AD and matched control cases...
2017: PloS One
https://www.readbyqxmd.com/read/28699896/eb1-protein-alteration-characterizes-sporadic-but-not-ulcerative-colitis-associated-colorectal-cancer
#6
Timo Gemoll, Sophie L Kollbeck, Karl F Karstens, Gia G Hò, Sonja Hartwig, Sarah Strohkamp, Katharina Schillo, Christoph Thorns, Martina Oberländer, Kathrin Kalies, Stefan Lehr, Jens K Habermann
BACKGROUND: While carcinogenesis in Sporadic Colorectal Cancer (SCC) has been thoroughly studied, less is known about Ulcerative Colitis associated Colorectal Cancer (UCC). This study aimed to identify and validate differentially expressed proteins between clinical samples of SCC and UCC to elucidate new insights of UCC/SCC carcinogenesis and progression. RESULTS: Multiplex-fluorescence two-dimensional gel electrophoresis (2-D DIGE) and mass spectrometry identified 67 proteoforms representing 43 distinct proteins...
July 4, 2017: Oncotarget
https://www.readbyqxmd.com/read/28698100/adenosquamous-gallbladder-carcinoma-multigene-hotspot-mutational-profiling-reveals-a-monoclonal-origin-of-the-two-components
#7
Francesca Galuppini, Roberta Salmaso, Elisa Valentini, Cristiano Lanza, Isacco Maretto, Donato Nitti, Massimo Rugge, Matteo Fassan
Adenosquamous carcinoma (ASC) of the gallbladder is a rare malignant tumor that is characterized by a coexisting of glandular and squamous components. In a case of ASC, we performed hotspot multigene mutational profiling of 164 hotspot regions of eleven cancer-associated genes (AKT1, APC, BRAF, CTNNB1, KIT, KRAS, NRAS, PDGFRA, PIK3CA, PTEN and TP53) in the two microdissected components. Both tumor phenotypes resulted characterized by a p.E542K point mutation in the PIK3CA gene, whereas adenocarcinoma component revealed also a TP53 Q331* homozygous stop mutation...
May 23, 2017: Pathology, Research and Practice
https://www.readbyqxmd.com/read/28696241/ethambutol-partitioning-in-tuberculous-pulmonary-lesions-explains-its-clinical-efficacy
#8
Matthew Zimmerman, Jodi Lestner, Brendan Prideaux, Paul O'Brien, Isabela Freedman, Chao Chen, Jillian Dietzold, Isaac Daudelin, Firat Kaya, Landry Blanc, Pei-Yu Chen, Steven Park, Padmini Salgame, Jansy Sarathy, Véronique Dartois
Clinical trials and practice have shown that ethambutol is an important component of the first line tuberculosis (TB) regime. This contrasts the drug's rather modest potency and lack of activity against non-growing persister mycobacteria. The standard plasma-based pharmacokinetic-pharmacodynamic profile of ethambutol suggests that the drug may be of limited clinical value. Here we hypothesized that this apparent contradiction may be explained by favorable penetration of the drug into TB lesions. First we utilized novel in vitro lesion pharmacokinetic assays and predicted good penetration of the drug into lesions...
July 10, 2017: Antimicrobial Agents and Chemotherapy
https://www.readbyqxmd.com/read/28687755/the-integrated-pathway-of-tgf%C3%AE-snail-with-tnf%C3%AE-nf%C3%AE%C2%BAb-may-facilitate-the-tumor-stroma-interaction-in-the-emt-process-and-colorectal-cancer-prognosis
#9
Hui Li, Anjing Zhong, Si Li, Xianwen Meng, Xue Wang, Fangying Xu, Maode Lai
Substantial evidence has shown that epithelial-mesenchymal transition (EMT) plays critical roles in colorectal cancer (CRC) development and prognosis. To uncover the pivotal regulators that function in the cooperative interactions between cancer cells and their microenvironment and consequently affect the EMT process, we carried out a systematic analysis and evaluated prognosis in CRC specimens. Tumor buds and their surrounding stroma were captured using laser microdissection. We used gene expression profiling, bioinformatics analysis and regulatory network construction for molecular selection...
July 7, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28687161/deregulation-of-mir-126-3p-in-basal-like-breast-cancers-stroma-and-its-clinical-significance
#10
Jing Li, Jin Liang Ping, Bo Ma, Ying Rong Chen, Li Qin Li
INTRODUCTION: The aim of this study was to investigate miR-126-3p expression in stroma and tumor cells of basal-like breast cancer tissues, in an effort to elucidate the potential effect of miR-126-3p on tumor microenvironment and progress of basal-like breast cancer. METHODS: Expression levels of miR-126-3p in 33 paired basal-like breast cancer tissues were assayed by real-time quantitative PCR. Tumor cells and normal epithelial cell were isolated from ten paired basal-like breast cancer tissues and matched adjacent tissues, separately, using laser capture microdissect(LCM)-based PCR method...
May 31, 2017: Pathology, Research and Practice
https://www.readbyqxmd.com/read/28679987/analysis-of-deposited-peptides-in-amyloid-lesions-of-al-amyloidosis-by-mass-spectrometry
#11
Toshiya Sugimoto, Masayoshi Tasaki, Taro Yamashita, Mitsuharu Ueda, Yukio Ando, Konen Obayashi, Yutaka Okuno, Hiroyuki Hata
Although immunoglobulin light chain (LC) plays a critical role in AL amyloidosis, detailed characteristics of deposited LC are not well known. In this study, LC peptides were analyzed by a combination of laser microdissection, liquid chromatography, and mass spectrometry (LC-MS/MS) in 65 patients with AL amyloidosis. Constant regions of LC were detected in all patients. Kappa- or lambda-types were detected in 20 and 45 patients, respectively. Various types of constant regions of LC were found; however, IGLC3 and IGKC4 were the most frequently detected...
2017: [Rinshō Ketsueki] the Japanese Journal of Clinical Hematology
https://www.readbyqxmd.com/read/28677147/looking-beyond-human-papillomavirus-hpv-genotype-16-and-18-defining-hpv-genotype-distribution-in-cervical-cancers-in-australia-prior-to-vaccination
#12
Julia Ml Brotherton, Sepehr N Tabrizi, Samuel Phillips, Jan Pyman, Alyssa M Cornall, Neil Lambie, Lyndal Anderson, Margaret Cummings, Diane Payton, James P Scurry, Marsali Newman, Raghwa Sharma, Marion Saville, Suzanne M Garland
Australia has implemented a high coverage HPV vaccination program but has not to date established the distribution of HPV types that occur in cervical cancers in Australia. This information is important for determining the potential for cervical cancer prevention with both current and broader spectrum HPV vaccines. We analysed 847 cervical cancers diagnosed 2005-2015 in tertiary centres in the three most populous Australian states with resolution of specimens containing multiple HPV types using laser-capture microdissection...
July 5, 2017: International Journal of Cancer. Journal International du Cancer
https://www.readbyqxmd.com/read/28676534/expression-patterns-of-henmt1-and-piwil1-in-human-testis-implications-for-transposon-expression
#13
Anna-Lena Hempfling, Shu Ly Lim, David Adelson, Jemma Evans, Anne E O'Connor, Zhipeng Qu, Sabine Kliesch, Wolfgang Weidner, M K O'Bryan, Martin Bergmann
This study aimed to define the expression patterns of HENMT1 and PIWI proteins in human testis and investigate their association with transposon expression, infertility sub-type or development of testicular germ cell tumours (TGCT). Testis biopsies showing normal spermatogenesis were used to identify normal localization patterns of HENMT1 and PIWIL1 by immunolocalisation and RT-PCR after laser microdissection. 222 testis biopsies representing normal spermatogenesis, hypospermatogenesis, spermatogenic arrests, Sertoli cell-only (SCO) and testicular germ cell tumours (TGCT) were analysed by RT-qPCR for expression of HENMT1/PIWIL1/PIWIL2/PIWIL3/PIWIL4 and LINE-1...
July 4, 2017: Reproduction: the Official Journal of the Society for the Study of Fertility
https://www.readbyqxmd.com/read/28673354/the-footprint-of-the-ageing-stroma-in-older-patients-with-breast-cancer
#14
Barbara Brouwers, Debora Fumagalli, Sylvain Brohee, Sigrid Hatse, Olivier Govaere, Giuseppe Floris, Kathleen Van den Eynde, Yacine Bareche, Patrick Schöffski, Ann Smeets, Patrick Neven, Diether Lambrechts, Christos Sotiriou, Hans Wildiers
BACKGROUND: Tumours are not only composed of malignant cells but also consist of a stromal micro-environment, which has been shown to influence cancer cell behaviour. Because the ageing process induces accumulation of senescent cells in the body, this micro-environment is thought to be different in cancers occurring in old patients compared with younger patients. More specifically, senescence-related fibroblastic features, such as the senescence-associated secretory profile (SASP) and the induction of autophagy, are suspected to stimulate tumour growth and progression...
July 3, 2017: Breast Cancer Research: BCR
https://www.readbyqxmd.com/read/28666423/precision-oncology-using-a-limited-number-of-cells-optimization-of-whole-genome-amplification-products-for-sequencing-applications
#15
Shonan Sho, Colin M Court, Paul Winograd, Sangjun Lee, Shuang Hou, Thomas G Graeber, Hsian-Rong Tseng, James S Tomlinson
BACKGROUND: Sequencing analysis of circulating tumor cells (CTCs) enables "liquid biopsy" to guide precision oncology strategies. However, this requires low-template whole genome amplification (WGA) that is prone to errors and biases from uneven amplifications. Currently, quality control (QC) methods for WGA products, as well as the number of CTCs needed for reliable downstream sequencing, remain poorly defined. We sought to define strategies for selecting and generating optimal WGA products from low-template input as it relates to their potential applications in precision oncology strategies...
July 1, 2017: BMC Cancer
https://www.readbyqxmd.com/read/28665959/increased-migration-of-antigen-presenting-cells-to-newly-formed-lymphatic-vessels-in-transplanted-kidneys-by-glycol-split-heparin
#16
Ditmer T Talsma, Kirankumar Katta, Miriam Boersema, Saritha Adepu, Annamaria Naggi, Giangiacomo Torri, Coen Stegeman, Gerjan Navis, Harry van Goor, Jan-Luuk Hillebrands, Saleh Yazdani, Jacob van den Born
BACKGROUND: Chronic renal transplant dysfunction is characterized by loss of renal function and tissue remodeling, including chronic inflammation and lymph vessel formation. Proteoglycans are known for their chemokine presenting capacity. We hypothesize that interruption of the lymphatic chemokine-proteoglycan interaction interferes with the lymphatic outflow of leukocytes from the renal graft and might decrease the anti-graft allo-immune response. METHODS: In a rat renal chronic transplant dysfunction model (female Dark-Agouti to male Wistar Furth), chemokines were profiled by qRT-PCR in microdissected tubulo-interstitial tissue...
2017: PloS One
https://www.readbyqxmd.com/read/28665924/somatic-pik3ca-mutations-are-present-in-multiple-tissues-of-facial-infiltrating-lipomatosis
#17
Javier A Couto, Dennis J Konczyk, Matthew P Vivero, Harry P W Kozakewich, Joseph Upton, Xi Fu, Bonnie L Padwa, John B Mulliken, Matthew L Warman, Arin K Greene
BACKGROUND: Facial infiltrating lipomatosis (FIL) is a congenital disorder that causes overgrowth of one side of the face. The purpose of this study was to determine if PIK3CA mutations are present in tissues outside of the subcutaneous adipose. METHODS: FIL tissues from 3 patients were dissected to enrich for cells from skin, subcutaneous tissue, orbicularis oris muscle, buccal fat, zygomatic bone, and mucosal neuroma. Endothelial cells within affected tissue also were enriched using CD31-microbeads...
June 30, 2017: Pediatric Research
https://www.readbyqxmd.com/read/28665451/discordance-in-ras-mutations-between-primary-colon-tumor-and-metastases-a-real-event-or-a-matter-of-methodology
#18
Alfonso De Stefano, Mario Rosanova, Umberto Malapelle, Maurizio Martini, Stefano De Falco, Laura Attademo, Giovanni Fiore, Tonia Cenci, Claudio Bellevicine, Sabino De Placido, Giancarlo Troncone, Chiara Carlomagno
BACKGROUND: Analysis of K- and N-RAS mutations is mandatory before planning treatment of metastatic colorectal cancer, because only RAS wild-type (WT) patients can benefit from treatment with anti-EGFR monoclonal antibodies (cetuximab and panitumumab). CASE REPORT: Here we report the case of a 69-year-old male patient affected by metastatic sigmoid cancer. He underwent left hemicolectomy, and histology diagnosed a well-differentiated, pT4, node-positive adenocarcinoma; KRAS analysis performed with direct sequencing identified a mutation in exon 2 of the KRAS gene (GGT->GTT)...
June 20, 2017: International Journal of Biological Markers
https://www.readbyqxmd.com/read/28664915/kinase-driven-metabolic-signalling-as-a-predictor-of-response-to-carboplatin-paclitaxel-adjuvant-treatment-in-advanced-ovarian-cancers
#19
Maria Isabella Sereni, Elisa Baldelli, Guido Gambara, Antonella Ravaggi, K Alex Hodge, David S Alberts, Jose M Guillen-Rodriguez, Ting Dong, Maurizio Memo, Franco Odicino, Roberto Angioli, Lance A Liotta, Sergio L Pecorelli, Emanuel F Petricoin, Mariaelena Pierobon
BACKGROUND: The biological mechanisms underlying early- and advanced-stage epithelial ovarian cancers (EOCs) are still poorly understood. This study explored kinase-driven metabolic signalling in early and advanced EOCs, and its role in tumour progression and response to carboplatin-paclitaxel treatment. METHODS: Tumour epithelia were isolated from two independent sets of primary EOC (n=72 and 30 for the discovery and the validation sets, respectively) via laser capture microdissection...
June 29, 2017: British Journal of Cancer
https://www.readbyqxmd.com/read/28663230/tissue-specific-microrna-expression-patterns-in-four-types-of-kidney-disease
#20
Maria Angeles Baker, Seth J Davis, Pengyuan Liu, Xiaoqing Pan, Anna Marie Williams, Kenneth A Iczkowski, Sean T Gallagher, Kaylee Bishop, Kevin R Regner, Yong Liu, Mingyu Liang
MicroRNAs contribute to the development of kidney disease. Previous analyses of microRNA expression in human kidneys, however, were limited by tissue heterogeneity or the inclusion of only one pathologic type. In this study, we used laser-capture microdissection to obtain glomeruli and proximal tubules from 98 human needle kidney biopsy specimens for microRNA expression analysis using deep sequencing. We analyzed specimens from patients with diabetic nephropathy (DN), FSGS, IgA nephropathy (IgAN), membranoproliferative GN (MPGN) (n=19-23 for each disease), and a control group (n=14)...
June 29, 2017: Journal of the American Society of Nephrology: JASN
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