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https://www.readbyqxmd.com/read/29932956/broad-spectrum-proteomics-analysis-of-the-inferior-colliculus-following-acute-hydrogen-sulfide-exposure
#1
Dong-Suk Kim, Poojya Anantharam, Andrea Hoffmann, Mitchell L Meade, Nadja Grobe, Jeffery M Gearhart, Elizabeth M Whitley, Belinda Mahama, Wilson K Rumbeiha
Acute exposure to high concentrations of H2 S causes severe brain injury and long-term neurological disorders, but the mechanisms involved are not known. To better understand the cellular and molecular mechanisms involved in acute H2 S-induced neurodegeneration we used a broad-spectrum proteomic analysis approach to identify key molecules and molecular pathways involved in the pathogenesis of acute H2 S-induced neurotoxicity and neurodegeneration. Mice were subjected to acute inhalation exposure of up to750 ppm of H2 S...
June 19, 2018: Toxicology and Applied Pharmacology
https://www.readbyqxmd.com/read/29931454/tissue-specific-chemical-profiling-and-quantitative-analysis-of-bioactive-components-of-cinnamomum-cassia-by-combining-laser-microdissection-with-uplc-q-tof-ms
#2
Wenwen Zhou, Zhitao Liang, Ping Li, Zhongzhen Zhao, Jun Chen
BACKGROUND: Cinnamomi Cortex, the dried stem bark of Cinnamomum cassia Presl (Rougui in Chinese) has been widely used in traditional Chinese medicine, cooking and perfumery for thousands of years. Traditionally, the Cinnamomi Cortex of thick size is considered to be of good quality; however, there is no scientific data to support this point. Considering that essential oils are the main bioactive components, Cinnamomi Cortex of greater variety and amount essential oils is thought to be of better quality...
June 21, 2018: Chemistry Central Journal
https://www.readbyqxmd.com/read/29930304/methodology-for-y-chromosome-capture-a-complete-genome-sequence-of-y-chromosome-using-flow-cytometry-laser-microdissection-and-magnetic-streptavidin-beads
#3
M J Alvarez-Cubero, O Santiago, C Martínez-Labarga, B Martínez-García, R Marrero-Díaz, A Rubio-Roldan, A M Pérez-Gutiérrez, P Carmona-Saez, J A Lorente, L J Martinez-Gonzalez
This study is a comparison of the efficiency of three technologies used for Y chromosome capture and the next-generation sequencing (NGS) technologies applied for determining its whole sequence. Our main findings disclose that streptavidin-biotin magnetic particle-based capture methodology offers better and a deeper sequence coverage for Y chromosome capture, compared to chromosome sorting and microdissection procedures. Moreover, this methodology is less time consuming and the most selective for capturing only Y chromosomal material, in contrast with other methodologies that result in considerable background material from other, non-targeted chromosomes...
June 21, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29929439/comparison-of-acute-gene-expression-profiles-of-islet-cells-obtained-via-laser-capture-microdissection-between-alloxan-and-streptozotocin-treated-rats
#4
Yuki Kato, Yusaku Masago, Chiaki Kondo, Erika Yogo, Mikinori Torii, Atsuko Hishikawa, Takeshi Izawa, Mitsuru Kuwamura, Jyoji Yamate
To identify the molecular profiles of islets from alloxan (ALX)- and streptozotocin (STZ)-treated rats, a microarray-based global gene expression analysis was performed on frozen islets isolated via laser capture microdissection. At 6 weeks old, rats were injected with ALX (40 mg/kg) or STZ (50 or 100 mg/kg) and then euthanized 24 hr later. Histopathological analysis showed β-cell necrosis, macrophage infiltration, and islet atrophy. The extent of these changes was more notable in the STZ groups than in the ALX group...
January 1, 2018: Toxicologic Pathology
https://www.readbyqxmd.com/read/29928911/cadm1-is-a-diagnostic-marker-in-early-stage-mycosis-fungoides-multicenter-study-of-58-cases
#5
Akihiko Yuki, Satoru Shinkuma, Ryota Hayashi, Hiroki Fujikawa, Taisuke Kato, Erina Homma, Yohei Hamade, Osamu Onodera, Masao Matsuoka, Hiroshi Shimizu, Hiroaki Iwata, Riichiro Abe
BACKGROUND: Mycosis fungoides (MF) is the most common cutaneous T-cell lymphoma (CTCL). Early-stage MF patches or plaques often resemble inflammatory skin disorders (ISDs), including psoriasis and atopic dermatitis. Cell adhesion molecule 1 (CADM1), initially identified as a tumor suppressor gene in human non-small-cell lung cancer, has been reported as a diagnostic marker for adult T-cell leukemia/lymphoma (ATLL). OBJECTIVE: We investigated CADM1 expression in MF neoplastic cells, especially during early stages, and evaluated its usefulness as a diagnostic marker for MF...
June 18, 2018: Journal of the American Academy of Dermatology
https://www.readbyqxmd.com/read/29921690/neoadjuvant-intensive-androgen-deprivation-therapy-selects-for-prostate-tumor-foci-with-diverse-subclonal-oncogenic-alterations
#6
Adam G Sowalsky, Huihui Ye, Manoj Bhasin, Eliezer M Van Allen, Massimo Loda, Rosina T Lis, Laleh Montaser-Kouhsari, Carla Calagua, Fen Ma, Joshua W Russo, Rachel J Schaefer, Olga S Voznesensky, Zhenwei Zhang, Glenn J Bubley, Bruce Montgomery, Elahe A Mostaghel, Peter S Nelson, Mary-Ellen Taplin, Steven P Balk
Primary prostate cancer (PCa) can have extensive microheterogeneity, but its contribution to the later emergence of metastatic castration-resistant PCa (mCRPC) remains unclear. In this study, we microdissected residual PCa foci in radical prostatectomies from 18 men treated with neoadjuvant intensive androgen deprivation therapy (leuprolide, abiraterone acetate, and prednisone) and analyzed them for resistance mechanisms. Transcriptome profiling showed reduced but persistent androgen receptor (AR) activity in residual tumors with no increase in neuroendocrine differentiation...
June 19, 2018: Cancer Research
https://www.readbyqxmd.com/read/29921228/laser-capture-microdissection-for-transcriptomic-profiles-in-human-skin-biopsies
#7
Silvia Santoro, Ignazio Diego Lopez, Raffaella Lombardi, Andrea Zauli, Ana Maria Osiceanu, Melissa Sorosina, Ferdinando Clarelli, Silvia Peroni, Daniele Cazzato, Margherita Marchi, Angelo Quattrini, Giancarlo Comi, Raffaele Adolfo Calogero, Giuseppe Lauria, Filippo Martinelli Boneschi
BACKGROUND: The acquisition of reliable tissue-specific RNA sequencing data from human skin biopsy represents a major advance in research. However, the complexity of the process of isolation of specific layers from fresh-frozen human specimen by laser capture microdissection, the abundant presence of skin nucleases and RNA instability remain relevant methodological challenges. We developed and optimized a protocol to extract RNA from layers of human skin biopsies and to provide satisfactory quality and amount of mRNA sequencing data...
June 19, 2018: BMC Molecular Biology
https://www.readbyqxmd.com/read/29920741/sperm-retrieval-rate-and-reproductive-outcome-of-infertile-men-with-azoospermia-factor-c-deletion
#8
Marjan Sabbaghian, Anahita Mohseni Meybodi, Alemeh Rafaee, Sadaf Saba, Mohammadreza Zamanian, Mohammad Ali Sadighi Gilani
To evaluate the success rate in sperm retrieval (SR) through microdissection testicular sperm extraction (micro-TESE) in infertile azoospermia factor c (AZFc)-deleted men and determining their reproductive outcomes following ICSI, medical records of couples with AZFc-deleted male partners were reviewed on patient's age, serum hormone levels, karyotype, testicular pathology and pregnancy outcomes. A comparison on age and serum hormone level was conducted between groups with positive and negative sperm retrieval in both azoospermic and oligozoospermic AZFc-deleted men...
June 19, 2018: Andrologia
https://www.readbyqxmd.com/read/29912187/laser-capture-microdissection-of-highly-pure-trabecular-meshwork-from-mouse-eyes-for-gene-expression-analysis
#9
Caleb Sutherland, Yu Wang, Robert V Brown, Julie Foley, Beth Mahler, Kyathanahalli S Janardhan, Ramesh C Kovi, Anton M Jetten
Laser capture microdissection (LCM) has allowed gene expression analysis of single cells and enriched cell populations in tissue sections. LCM is a great tool for the study of the molecular mechanisms underlying cell differentiation and the development and progression of various diseases, including glaucoma. Glaucoma, which comprises a family of progressive optic neuropathies, is the most common cause of irreversible blindness worldwide. Structural changes and damage within the trabecular meshwork (TM) can result in increased intraocular pressure (IOP), which is a major risk factor for developing glaucoma...
June 3, 2018: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/29899869/direct-molecular-dissection-of-tumor-parenchyma-from-tumor-stroma-in-tumor-xenograft-using-mass-spectrometry-based-glycoproteomics
#10
Xiaoying Ye, Brian T Luke, Bih-Rong Wei, Jan A Kaczmarczyk, Jadranka Loncarek, Jennifer E Dwyer, Donald J Johann, Richard G Saul, Dwight V Nissley, Frank McCormick, Gordon R Whiteley, Josip Blonder
The most widely used cancer animal model is the human-murine tumor xenograft. Unbiased molecular dissection of tumor parenchyma versus stroma in human-murine xenografts is critical for elucidating dysregulated protein networks/pathways and developing therapeutics that may target these two functionally codependent compartments. Although antibody-reliant technologies (e.g., immunohistochemistry, imaging mass cytometry) are capable of distinguishing tumor-proper versus stromal proteins, the breadth or extent of targets is limited...
May 29, 2018: Oncotarget
https://www.readbyqxmd.com/read/29892011/transcriptome-analysis-highlights-nuclear-control-of-chloroplast-development-in-the-shoot-apex
#11
Vijay Dalal, Shlomi Dagan, Gilgi Friedlander, Elinor Aviv, Ralph Bock, Dana Charuvi, Ziv Reich, Zach Adam
In dicots, the key developmental process by which immature plastids differentiate into photosynthetically competent chloroplasts commences in the shoot apical meristem (SAM), within the shoot apex. Using laser-capture microdissection and single-cell RNA sequencing methodology, we studied the changes in the transcriptome along the chloroplast developmental pathway in the shoot apex of tomato seedlings. The analysis revealed the presence of transcripts for different chloroplast functions already in the stem cell-containing region of the SAM...
June 11, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29888431/differential-proteomic-analysis-between-small-cell-lung-carcinoma-sclc-and-pulmonary-carcinoid-tumors-reveals-molecular-signatures-for-malignancy-in-lung-cancer
#12
Kiyonaga Fujii, Yuka Miyata, Ikuya Takahashi, Hirotaka Koizumi, Hisashi Saji, Masahiro Hoshikawa, Masayuki Takagi, Toshihide Nishimura, Haruhiko Nakamura
PURPOSE: The molecular underpinnings that may prognosticate survival and increase our understanding of tumor development and progression are still poorly understood. This study aimed to define the molecular signatures for malignancy in small cell lung carcinoma (SCLC), which is known for its highly aggressive clinical features and poor prognosis. EXPERIMENTAL DESIGN: Using clinical specimens, we performed a comparative proteomic analysis of high-grade SCLCs and low-grade pulmonary carcinoid tumors (PCTs), both of which are types of neuroendocrine tumors...
June 11, 2018: Proteomics. Clinical Applications
https://www.readbyqxmd.com/read/29887316/palmitate-deranges-erythropoietin-production-via-transcription-factor-atf4-activation-of-unfolded-protein-response
#13
Thitinun Anusornvongchai, Masaomi Nangaku, Tzu-Ming Jao, Chia-Hsien Wu, Yu Ishimoto, Hiroshi Maekawa, Tetsuhiro Tanaka, Akira Shimizu, Masayuki Yamamoto, Norio Suzuki, Ryoji Sassa, Reiko Inagi
Lipotoxicity plays an important role in the progression of chronic kidney damage via various mechanisms, such as endoplasmic reticulum stress. Several studies proposed renal lipotoxicity in glomerular and tubular cells but the effect of lipid on renal erythropoietin (EPO)-producing (REP) cells in the interstitium has not been elucidated. Since renal anemia is caused by derangement of EPO production in REP cells, we evaluated the effect of palmitate, a representative long-chain saturated fatty acid, on EPO production and the endoplasmic reticulum stress pathway...
June 7, 2018: Kidney International
https://www.readbyqxmd.com/read/29884700/deep-sequencing-reveals-transient-segregation-of-t-cell-repertoires-in-splenic-t-cell-zones-during-an-immune-response
#14
Johannes Textor, Anke Fähnrich, Martin Meinhardt, Cornelia Tune, Sebastian Klein, Rene Pagel, Peter König, Kathrin Kalies, Jürgen Westermann
Immunological differences between hosts, such as diverse TCR repertoires, are widely credited for reducing the risk of pathogen spread and adaptation in a population. Within-host immunological diversity might likewise be important for robust pathogen control, but to what extent naive TCR repertoires differ across different locations in the same host is unclear. T cell zones (TCZs) in secondary lymphoid organs provide secluded microenvironmental niches. By harboring distinct TCRs, such niches could enhance within-host immunological diversity...
June 8, 2018: Journal of Immunology: Official Journal of the American Association of Immunologists
https://www.readbyqxmd.com/read/29883638/a-comprehensive-spatial-temporal-transcriptomic-analysis-of-differentiating-nascent-mouse-lens-epithelial-and-fiber-cells
#15
Yilin Zhao, Deyou Zheng, Ales Cvekl
Elucidation of both the molecular composition and organization of the ocular lens is a prerequisite to understand its development, function, pathology, regenerative capacity, as well as to model lens development and disease using in vitro differentiation of pluripotent stem cells. Lens is comprised of the anterior lens epithelium and posterior lens fibers, which form the bulk of the lens. Lens fibers differentiate from lens epithelial cells through cell cycle exit-coupled differentiation that includes cellular elongation, accumulation of crystallins, cytoskeleton and membrane remodeling, and degradation of organelles within the central region of the lens...
June 5, 2018: Experimental Eye Research
https://www.readbyqxmd.com/read/29881994/transcriptome-pathology-correlation-identifies-interplay-between-tdp-43-and-the-expression-of-its-kinase-ck1e-in-sporadic-als
#16
Florian Krach, Ranjan Batra, Emily C Wheeler, Anthony Q Vu, Ruth Wang, Kasey Hutt, Stuart J Rabin, Michael W Baughn, Ryan T Libby, Sandra Diaz-Garcia, Jennifer Stauffer, Elaine Pirie, Shahram Saberi, Maria Rodriguez, Assael A Madrigal, Zacharias Kohl, Beate Winner, Gene W Yeo, John Ravits
Sporadic amyotrophic lateral sclerosis (sALS) is the most common form of ALS, however, the molecular mechanisms underlying cellular damage and motor neuron degeneration remain elusive. To identify molecular signatures of sALS we performed genome-wide expression profiling in laser capture microdissection-enriched surviving motor neurons (MNs) from lumbar spinal cords of sALS patients with rostral onset and caudal progression. After correcting for immunological background, we discover a highly specific gene expression signature for sALS that is associated with phosphorylated TDP-43 (pTDP-43) pathology...
June 7, 2018: Acta Neuropathologica
https://www.readbyqxmd.com/read/29879699/differentiation-and-evolution-of-the-w-chromosome-in-the-fish-species-of-megaleporinus-characiformes-anostomidae
#17
Lucas Caetano de Barros, Diovani Piscor, Patricia P Parise-Maltempi, Eliana Feldberg
The W chromosome of Megaleporinus trifasciatus was isolated in order to analyze its behavior in the karyotype of this and other species of the family, including forms with differentiated and undifferentiated sex chromosomes. The chromosome was microdissected, and the WMt probe was prepared for the chromosome painting procedure. M. trifasciatus was also cross-hybridized (cross-FISH) using existing probes available for M. macrocephalus (WMm) and M. elongatus (WMe). Two Leporinus species and Semaprochilodus taeniurus, representing a clade close to the Anostomidae, were also cross-hybridized with the objective to better understand the evolution of the sex chromosomes...
June 8, 2018: Sexual Development: Genetics, Molecular Biology, Evolution, Endocrinology, Embryology, and Pathology of Sex Determination and Differentiation
https://www.readbyqxmd.com/read/29879393/bnip3l-nix-is-required-for-elimination-of-mitochondria-endoplasmic-reticulum-and-golgi-apparatus-during-eye-lens-organelle-free-zone-formation
#18
Lisa A Brennan, Rebecca McGreal-Estrada, Caitlin M Logan, Ales Cvekl, A Sue Menko, Marc Kantorow
The formation and life-long growth of the ocular lens depends on the continuous differentiation of lens epithelial cells into lens fiber cells. To achieve their mature structure and transparent function, newly formed lens fiber cells undergo a series of cellular remodeling events including the complete elimination of cellular organelles to form the lens organelle-free zone (OFZ). To date, the mechanisms and requirements for organelle elimination by lens fiber cells remain to be fully elucidated. In previous studies, we detected the presence of mitochondria contained within autophagolysosomes throughout human and chick lenses suggesting that proteins targeting mitochondria for degradation by mitophagy could be required for the elimination of mitochondria during OFZ formation...
June 4, 2018: Experimental Eye Research
https://www.readbyqxmd.com/read/29879147/interrogation-of-transcriptomic-changes-associated-with-drug-induced-hepatic-sinusoidal-dilatation-in-colorectal-cancer
#19
Monika A Jarzabek, William R Proctor, Jennifer Vogt, Rupal Desai, Patrick Dicker, Gary Cain, Rajiv Raja, Jens Brodbeck, Dale Stevens, Eric P van der Stok, John W M Martens, Cornelis Verhoef, Priti S Hegde, Annette T Byrne, Jacqueline M Tarrant
Drug-related sinusoidal dilatation (SD) is a common form of hepatotoxicity associated with oxaliplatin-based chemotherapy used prior to resection of colorectal liver metastases (CRLM). Recently, hepatic SD has also been associated with anti-delta like 4 (DLL4) cancer therapies targeting the NOTCH pathway. To investigate the hypothesis that NOTCH signaling plays an important role in drug-induced SD, gene expression changes were examined in livers from anti-DLL4 and oxaliplatin-induced SD in non-human primate (NHP) and patients, respectively...
2018: PloS One
https://www.readbyqxmd.com/read/29873224/quantification-of-biogenic-amines-from-individual-gfp-labelled-drosophila-cells-by-maldi-tof-mass-spectrometry
#20
Max Diesner, Susanne Neupert
Cell-cell communication plays a crucial role in orchestrating and modulating neural circuits. To understand such interactions, it is vital to determine and quantify the involved messenger molecules such as neuropeptides and biogenic amines on the level of single cells. In this study, we used single-cell mass spectrometry (SCMS) to qualify and quantify octopamine (OA) and tyramine (TA) from isolated single cells from intact brains of the fruit fly Drosophila melanogaster. Our workflow involved targeted GFP-guided single-cell microdissection, on-plate chemical derivatization with 4-hydroxy-3-methoxycinnamaldehyde (CA) or 2,5-dimethyl-1H-pyrrole-3,4-dicarbaldehyde (DPD) for increasing ion stability and ion signal intensity, and isotopically marked internal standards for quantification by MALDI-TOF MS...
June 6, 2018: Analytical Chemistry
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