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https://www.readbyqxmd.com/read/28644127/mpi-depletion-enhances-o-glcnacylation-of-p53-and-suppresses-the-warburg-effect
#1
Charles DeRossi, Nataly Shtraizent, Shikha Nayar, Ravi Sachidanandam, Liora S Katz, Adam Prince, Anna P Koh, Adam Vincek, Yoav Hadas, Yujin Hoshida, Donald K Scott, Efrat Eliyahu, Hudson H Freeze, Kirsten C Sadler, Jaime Chu
Rapid cellular proliferation in early development and cancer depends on glucose metabolism to fuel macromolecule biosynthesis. Metabolic enzymes are presumed regulators of this glycolysis-driven metabolic program, known as the Warburg effect, however few have been identified. We uncover a previously unappreciated role for Mannose phosphate isomerase (MPI) as a metabolic enzyme required to maintain Warburg metabolism in zebrafish embryos and in both primary and malignant mammalian cells. The functional consequences of MPI loss are striking: glycolysis is blocked and cells die...
June 23, 2017: ELife
https://www.readbyqxmd.com/read/28642062/lrh-1-senses-signaling-from-phosphatidylcholine-to-regulate-the-expansion-growth-of-digestive-organs-via-synergy-with-wnt-%C3%AE-catenin-signaling-in-zebrafish
#2
Gang Zhai, Jia Song, Tingting Shu, Junjun Yan, Xia Jin, Jiangyan He, Zhan Yin
Liver receptor homolog-1 (LRH-1) is an orphan nuclear receptor that is critical for the growth and proliferation of cancer cells and other biological processes, including lipid transportation and metabolism, sexual determination and steroidogenesis. However, because homozygous lrh-1(-/-) mice die in utero, the regulatory mechanisms involved in embryonic development mediated by this receptor are poorly understood. In the present study, we performed transcription activator-like effector nuclease (TALEN)-mediated loss-of-function assays, taking advantage of zebrafish external fertilization, to investigate the function of lrh-1...
April 27, 2017: Journal of Genetics and Genomics, Yi Chuan Xue Bao
https://www.readbyqxmd.com/read/28621412/the-not-so-long-history-of-zebrafish-research-in-israel
#3
Janna Blechman, Gil Levkowitz, Yoav Gothilf
The zebrafish has become a model of choice in fundamental and applied life sciences and is widely used in various fields of biomedical research as a human disease model for cancer, metabolic and neurodegenerative diseases, and regenerative medicine. The transparency of the zebrafish embryo allows real-time visualization of the development and morphogenesis of practically all of its tissues and organs. Zebrafish are amenable to genetic manipulation, for which innovative genetic and molecular techniques are constantly being introduced...
2017: International Journal of Developmental Biology
https://www.readbyqxmd.com/read/28614716/the-notch1-snail1-mef2c-pathway-regulates-growth-and-self-renewal-in-embryonal-rhabdomyosarcoma
#4
Myron S Ignatius, Madeline N Hayes, Riadh Lobbardi, Eleanor Y Chen, Karin M McCarthy, Prethish Sreenivas, Zainab Motala, Adam D Durbin, Aleksey Molodtsov, Sophia Reeder, Alexander Jin, Sivasish Sindiri, Brian C Beleyea, Deepak Bhere, Matthew S Alexander, Khalid Shah, Charles Keller, Corinne M Linardic, Petur G Nielsen, David Malkin, Javed Khan, David M Langenau
Tumor-propagating cells (TPCs) share self-renewal properties with normal stem cells and drive continued tumor growth. However, mechanisms regulating TPC self-renewal are largely unknown, especially in embryonal rhabdomyosarcoma (ERMS)-a common pediatric cancer of muscle. Here, we used a zebrafish transgenic model of ERMS to identify a role for intracellular NOTCH1 (ICN1) in increasing TPCs by 23-fold. ICN1 expanded TPCs by enabling the de-differentiation of zebrafish ERMS cells into self-renewing myf5+ TPCs, breaking the rigid differentiation hierarchies reported in normal muscle...
June 13, 2017: Cell Reports
https://www.readbyqxmd.com/read/28611298/tumor-diversity-and-evolution-revealed-through-radseq
#5
Elizabeth B Perry, Alvin Makohon-Moore, Caihong Zheng, Charles K Kaufman, Jun Cai, Christine A Iacobuzio-Donahue, Richard M White
Cancer is an evolutionary disease, and there is increasing interest in applying tools from evolutionary biology to understand cancer progression. Restriction-site associated DNA sequencing (RADseq) was developed for the field of evolutionary genetics to study adaptation and identify evolutionary relationships among populations. Here we apply RADseq to study tumor evolution, which allows for unbiased sampling of any desired frequency of the genome, overcoming the selection bias and cost limitations inherent to exome or whole-genome sequencing...
June 3, 2017: Oncotarget
https://www.readbyqxmd.com/read/28606834/greb1-regulates-convergent-extension-movement-and-pituitary-development-in-zebrafish
#6
Shi-Zhu Li, Wei Liu, Zhi Li, Wen-Hua Li, Yang Wang, Li Zhou, Jian-Fang Gui
greb1, growth regulation by estrogen in breast cancer 1, is an important estrogen response gene, which participates in breast cancer formation. However, the role of greb1 in development is largely unknown. In this study, we have characterized greb1 expression patterns and revealed its regulative role in convergent extension movement and pituitary development in zebrafish. In zebrafish adults, greb1 was revealed to be expressed mainly in pituitary, hypothalamus, liver, and gonads. During embryogenesis, RT-PCR and WISH revealed its spatial and temporal expression specific to embryonic margin during gastrulation, central nervous system during segmentation and brain ventricular regions from 24hpf...
June 9, 2017: Gene
https://www.readbyqxmd.com/read/28602865/anti-angiogenic-activity-of-water-extract-from-euphorbia-pekinensis-rupr
#7
Wenting Zhang, Bin Liu, Yaru Feng, Jie Liu, Zhiqiang Ma, Jian Zheng, Qing Xia, Yuanyuan Ni, Farong Li, Ruichao Lin
ETHNOPHARMACOLOGICAL RELEVANCE: Euphorbia pekinensis Rupr. (EP) is a Euphorbia species of Euphorbiaceae, which is widely used in traditional Chinese medicine. It has been reported to exhibit therapeutic effects on solid tumors, leukemias, and malignant ascites although underlying molecular mechanisms are poorly delineated. Anti-angiogenic therapy is a recognized strategy for treating cancer-based solid tumors, and is also associated with malignant ascites treatment. STUDY AIM: To study the anti-angiogenic properties of the water extract of EP vinegar preparation (WEVEP)...
June 7, 2017: Journal of Ethnopharmacology
https://www.readbyqxmd.com/read/28589491/synthesis-and-characterization-of-novel-bmi1-inhibitors-targeting-cellular-self-renewal-in-hepatocellular-carcinoma
#8
Monica Bartucci, Mohamed S Hussein, Eric Huselid, Kathleen Flaherty, Michele Patrizii, Saurabh V Laddha, Cindy Kui, Rachel A Bigos, John A Gilleran, Mervat M S El Ansary, Mona A M Awad, S David Kimball, David J Augeri, Hatem E Sabaawy
BACKGROUND: Hepatocellular carcinoma (HCC) represents one of the most lethal cancers worldwide due to therapy resistance and disease recurrence. Tumor relapse following treatment could be driven by the persistence of liver cancer stem-like cells (CSCs). The protein BMI1 is a member of the polycomb epigenetic factors governing cellular self-renewal, proliferation, and stemness maintenance. BMI1 expression also correlates with poor patient survival in various cancer types. OBJECTIVE: We aimed to elucidate the extent to which BMI1 can be used as a potential therapeutic target for CSC eradication in HCC...
June 6, 2017: Targeted Oncology
https://www.readbyqxmd.com/read/28585811/dinuclear-ru-ii-bipy-2-derivatives-structural-biological-and-in-vivo-zebrafish-toxicity-evaluation
#9
Oscar A Lenis-Rojas, Catarina Roma-Rodrigues, Alexandra R Fernandes, Fernanda Marques, David Pérez-Fernández, Jorge Guerra-Varela, Laura Sánchez, Digna Vázquez-García, Margarita López-Torres, Alberto Fernández, Jesús J Fernández
Ruthenium-based drugs exhibit interesting properties as potential anticancer pharmaceuticals. We herein present the synthesis and characterization of a new family of ruthenium complexes with formulas [{Ru(bipy)2}2(μ-L)][CF3SO3]4 (L = bptz, 1a) and [{Ru(bipy)2}2(μ-L)][CF3SO3]2 (L = arphos, 2a; dppb, 3a; dppf, 4a), which were synthesized from the Ru(II) precursor compound cis-Ru(bipy)2Cl2. The complexes were characterized by elemental analysis, mass spectrometry, (1)H and (31)P{(1)H} NMR, IR spectroscopy, and conductivity measurements...
June 6, 2017: Inorganic Chemistry
https://www.readbyqxmd.com/read/28579318/the-zebrafish-kidney-mutant-zeppelin-reveals-that-brca2-fancd1-is-essential-for-pronephros-development
#10
Paul T Kroeger, Bridgette E Drummond, Rachel Miceli, Michael McKernan, Gary F Gerlach, Amanda N Marra, Annemarie Fox, Kristen K McCampbell, Ignaty Leshchiner, Adriana Rodriguez-Mari, Ruth BreMiller, Ryan Thummel, Alan J Davidson, John Postlethwait, Wolfram Goessling, Rebecca A Wingert
The zebrafish kidney is conserved with other vertebrates, making it an excellent genetic model to study renal development. The kidney collects metabolic waste using a blood filter with specialized epithelial cells known as podocytes. Podocyte formation is poorly understood but relevant to many kidney diseases, as podocyte injury leads to progressive scarring and organ failure. zeppelin (zep) was isolated in a forward screen for kidney mutants and identified as a homozygous recessive lethal allele that causes reduced podocyte numbers, deficient filtration, and fluid imbalance...
June 1, 2017: Developmental Biology
https://www.readbyqxmd.com/read/28574600/diallyl-trisulfides-a-natural-histone-deacetylase-inhibitor-attenuate-hif-1%C3%AE-synthesis-and-decreases-breast-cancer-metastasis
#11
Zhonghong Wei, Yunlong Shan, Li Tao, Yuping Liu, Zhijie Zhu, Zhaoguo Liu, Yuanyuan Wu, Wenxing Chen, Aiyun Wang, Yin Lu
Intratumoral hypoxia promotes the distant metastasis of cancer subclones. The clinical expression level of hypoxia-inducible factor-1α (HIF-1α) reflects the prognosis of a variety of cancers, especially breast cancer. Histone deacetylase (HDAC) inhibitors can target HIF-1α protein due to von Hippel-Lindau protein-dependent degradation. Dietary organosulfur compounds, such as those in garlic, have been reported as HDAC inhibitors. The effects of diallyl sulfide (DAS), diallyl disulfide (DADS) and diallyl trisulfide (DATS) on the ratio of firefly/Renilla luciferase activity in hypoxic MDA-MB-231 cells were determined...
June 2, 2017: Molecular Carcinogenesis
https://www.readbyqxmd.com/read/28570545/transplantation-of-zebrafish-pediatric-brain-tumors-into-immune-competent-hosts-for-long-term-study-of-tumor-cell-behavior-and-drug-response
#12
Mattie J Casey, Katarzyna Modzelewska, Daniela Anderson, James Goodman, Elena F Boer, Laura Jimenez, Douglas Grossman, Rodney A Stewart
Tumor cell transplantation is an important technique to define the mechanisms controlling cancer cell growth, migration, and host response, as well as to assess potential patient response to therapy. Current methods largely depend on using syngeneic or immune-compromised animals to avoid rejection of the tumor graft. Such methods require the use of specific genetic strains that often prevent the analysis of immune-tumor cell interactions and/or are limited to specific genetic backgrounds. An alternative method in zebrafish takes advantage of an incompletely developed immune system in the embryonic brain before 3 days, where tumor cells are transplanted for use in short-term assays (i...
May 17, 2017: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/28570143/tank-to-bedside-zebrafish-and-the-cancer-moonshot
#13
Lisa A Schimmenti
No abstract text is available yet for this article.
June 1, 2017: Zebrafish
https://www.readbyqxmd.com/read/28562620/reprimo-tissue-specific-expression-pattern-is-conserved-between-zebrafish-and-human
#14
Ricardo J Figueroa, Gonzalo Carrasco-Avino, Ignacio A Wichmann, Martin Lange, Gareth I Owen, Arndt F Siekmann, Alejandro H Corvalán, Juan C Opazo, Julio D Amigo
Reprimo (RPRM), a member of the RPRM gene family, is a tumor-suppressor gene involved in the regulation of the p53-mediated cell cycle arrest at G2/M. RPRM has been associated with malignant tumor progression and proposed as a potential biomarker for early cancer detection. However, the expression and role of RPRM, as well as its family, are poorly understood and their physiology is as yet unstudied. In this scenario, a model system like the zebrafish could serve to dissect the role of the RPRM family members in vivo...
2017: PloS One
https://www.readbyqxmd.com/read/28557653/efficient-transduction-of-zebrafish-melanoma-cell-lines-and-embryos-using-lentiviral-vectors
#15
Maurizio Fazio, Serine Avagyan, Ellen van Rooijen, William Mannherz, Charles K Kaufman, Riadh Lobbardi, David M Langenau, Leonard I Zon
The establishment of in vitro cultures of zebrafish cancer cells has expanded the potential of zebrafish as a disease model. However, the lack of effective methods for gene delivery and genetic manipulation has limited the experimental applications of these cultures. To overcome this barrier, we tested and optimized vesicular stomatitis virus glycoprotein (VSV-G) pseudotyped lentiviral and retroviral vector transduction protocols. We show that lentivirus successfully and efficiently transduced zebrafish melanoma cell lines in vitro, allowing antibiotic selection, fluorescence-based sorting, and in vivo allotransplantation...
May 30, 2017: Zebrafish
https://www.readbyqxmd.com/read/28557449/antimalarial-activities-of-alkyl-cyclohexenone-derivatives-isolated-from-the-leaves-of-poupartia-borbonica
#16
Allison Ledoux, Alexis St-Gelais, Ewa Cieckiewicz, Olivia Jansen, Annélise Bordignon, Bertrand Illien, Nicolas Di Giovanni, Arnaud Marvilliers, Floriane Hoareau, Hélène Pendeville, Joëlle Quetin-Leclercq, Michel Frédérich
Bioactivity-guided fractionation of the ethyl acetate extract of the leaves of Poupartia borbonica led to the isolation of three new alkyl cyclohexenone derivatives 1-3, and named Poupartone A-C. The structures of the new compounds were elucidated by 1D and 2D NMR spectroscopic data analysis and MS, whereas calculated and experimental ECD spectra were used to define the absolute configurations. These compounds were active against 3D7 and W2 Plasmodium falciparum strains with IC50 values between 0.55 and 1.81 μM...
May 30, 2017: Journal of Natural Products
https://www.readbyqxmd.com/read/28548928/crosstalk-between-tongue-carcinoma-cells-extracellular-vesicles-and-immune-cells-in-in-vitro-and-in-vivo-models
#17
Ahmed Al-Samadi, Shady Adnan Awad, Katja Tuomainen, Yue Zhao, Abdelhakim Salem, Mataleena Parikka, Tuula Salo
The crosstalk between immune cells, cancer cells, and extracellular vesicles (EVs) secreted by cancer cells remains poorly understood. We created three-dimensional (3D) cell culture models using human leiomyoma discs and Myogel to study the effects of immune cells on highly (HSC-3) and less (SCC-25) invasive oral tongue squamous cell carcinoma (OTSCC) cell lines. Additionally, we studied the effects of EVs isolated from these cell lines on the cytotoxicity of CD8+ T and NK cells isolated from three healthy donors...
May 10, 2017: Oncotarget
https://www.readbyqxmd.com/read/28534941/two-natural-eudesmane-type-sesquiterpenes-from-laggera-alata-inhibit-angiogenesis-and-suppress-breast-cancer-cell-migration-through-vegf-and-angiopoietin-2-mediated-signaling-pathways
#18
Ning Liang, Yaolan Li, Hau Yin Chung
Eudesmane-type sesquiterpenes are natural sesquiterpenes with anti-inflammatory properties, but their anti-angiogenic activities are not known. The present study demonstrated that 5α-hydroxycostic acid and hydroxyisocostic acid, two eudesmane-type sesquiterpenes (ETSs), isolated from the herb Laggera alata, possessed anti-angiogenic effects. Under non-toxic dosage, ETSs suppressed VEGF‑induced proliferation in human umbilical vein endothelial cells (HUVECs) and vessel formation in zebrafish embryos. Moreover, ETSs inhibited VEGF-stimulated HUVEC migration, stress fibers and tube formation...
May 17, 2017: International Journal of Oncology
https://www.readbyqxmd.com/read/28526577/changes-in-tumor-cell-heterogeneity-after-chemotherapy-treatment-in-a-xenograft-model-of-glioblastoma
#19
Alessandra M Welker, Brian D Jaros, Min An, Christine E Beattie
Glioblastoma (GBM) is a highly aggressive brain cancer with limited treatments and poor patient survival. GBM tumors are heterogeneous containing a complex mixture of dividing cells, differentiated cells, and cancer stem cells. It is unclear, however, how these different cell populations contribute to tumor growth or whether they exhibit differential responses to chemotherapy. Here we set out to address these questions using a zebrafish xenograft transplant model (Welker et al., 2016). We found that a small population of differentiated vimentin-positive tumor cells, but a majority of Sox2-positive putative cancer stem cells, were dividing during tumor growth...
May 17, 2017: Neuroscience
https://www.readbyqxmd.com/read/28525947/dianhydrogalactitol-a-potential-multitarget-agent-inhibits-glioblastoma-migration-invasion-and-angiogenesis
#20
Xia Jiang, Yinmei Huang, Xiaojie Wang, Qiaofang Liang, Yingjie Li, Fuji Li, Xuanhao Fu, Chonghuan Huang, Huagang Liu
The complexity of cancer has led to single-target agents exhibiting lower-than-desired clinical efficacy. Drugs with multiple targets provide a feasible option for the treatment of complex tumors. Multitarget anti-angiogenesis agents are among the new generation of anticancer drugs and have shown favorable clinical efficacy. Dianhydrogalactitol (DAG) is a chemotherapeutic agent for chronic myeloid leukemia and lung cancer. Recently, it has been tested in phase II trials of glioblastoma treatment; however, mechanisms of DAG in glioblastoma have not been elucidated...
July 2017: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
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