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https://www.readbyqxmd.com/read/29223587/molecular-characterization-of-theileria-equi-in-horses-from-the-state-of-rio-de-janeiro-brazil
#1
Maristela Peckle, Marcus Sandes Pires, Claudia Bezerra da Silva, Renata Lins da Costa, Gabriela Lopes Vivas Vitari, Marcus Vinicius Xavier Senra, Roberto Júnio Pedroso Dias, Huarrisson Azevedo Santos, Carlos Luiz Massard
Theileria equi is one of the etiologic agents of the equine piroplasmosis. This infectious disease is transmitted by ticks and is a worldwide problem in the international horse movement. The 18S rRNA gene of T. equi is often used for genotyping and phylogenetic purpose. This study aimed to analyze the degree of the heterogeneity of the 18S rRNA gene of T. equi in horses from the state of Rio de Janeiro, Brazil. The complete T. equi 18S rRNA sequences were obtained from twenty naturally infected horses. The PCR amplicons were cloned and sequenced...
November 28, 2017: Ticks and Tick-borne Diseases
https://www.readbyqxmd.com/read/29197596/historical-review-and-insights-on-the-livestock-tick-borne-disease-research-of-a-developing-country-the-philippine-scenario
#2
REVIEW
Adrian P Ybañez, Claro N Mingala, Rochelle Haidee D Ybañez
Tick-borne diseases (TBDs) remain to be a global animal health threat. Developing countries like the Philippines is not exempt to this. Despite the potential impact TBDs can give to these countries, local government initiatives and researches remain to be limited. In the Philippines, most epidemiological studies were confined only to specific areas, and predominantly in the Northern Area. Due to its unique geography and limited studies, the current nationwide status of most TBDs could not be clearly established...
November 29, 2017: Parasitology International
https://www.readbyqxmd.com/read/29173535/ticks-circulate-anaplasma-ehrlichia-babesia-and-theileria-parasites-in-north-of-iran
#3
Ahmad Jafar Bekloo, Hasan Bakhshi, Ayoub Soufizadeh, Mohammad Mehdi Sedaghat, Romina Jafar Bekloo, Maryam Roya Ramzgouyan, Asadollah Hosseini Chegeni, Faezeh Faghihi, Zakkyeh Telmadarraiy
Ticks serve as important vectors of some pathogens of medical importance all over the world and identification of their rate of infection plays an important role for further control of diseases. In the current study, we investigated on ticks collected from north of Iran where raising and caring livestock are the main task of the people in order to find evidences of infection of Babesia, Theileria, Anaplasma and Ehrlichia microbial agents. Totally, 609 hard tick species from two genera Hyalomma and Rhipicephalus including; Hy...
December 15, 2017: Veterinary Parasitology
https://www.readbyqxmd.com/read/29155863/molecular-detection-of-tick-borne-pathogens-in-cattle-from-southwestern-ethiopia
#4
Zerihun Hailemariam, Jürgen Krücken, Maximilian Baumann, Jabbar S Ahmed, Peter-Henning Clausen, Ard M Nijhof
Tick-borne diseases (TBDs) cause significant losses among livestock and impact the livelihoods of resource-poor farming communities worldwide. In Ethiopia, detailed studies on the epidemiology of tick-borne pathogens (TBPs) in cattle using sensitive molecular detection methods are scarce. The objective of this study was to determine the prevalence and species composition of bovine TBPs of veterinary significance in local cattle populations. A comprehensive cross-sectional epidemiological study was conducted in cattle populations of Illubabor zone in Southwestern Ethiopia from June to August 2013...
2017: PloS One
https://www.readbyqxmd.com/read/29155280/molecular-analysis-of-tick-borne-protozoan-and-rickettsial-pathogens-in-small-ruminants-from-two-south-african-provinces
#5
Aaron Edmond Ringo, Paul Franck Adjou Moumouni, Moeti Taioe, Charoonluk Jirapattharasate, Mingming Liu, Guanbo Wang, Yang Gao, Huanping Guo, Seung-Hun Lee, Weiqing Zheng, Artemis Efstratiou, Jixu Li, Noboru Inoue, Hiroshi Suzuki, Oriel Thekisoe, Xuenan Xuan
Tick-borne protozoan and rickettsial diseases are a major threat to livestock in tropical and sub-tropical regions of Africa. In this study we investigated the presence and distribution of Theileria spp., Babesia ovis, Anaplasma ovis, Anaplasma phagocytophilum, Ehrlichia ruminantium and SFG Rickettsia in sheep and goats from Free State and KwaZulu-Natal provinces. A total of 91 blood samples were screened in this study, 61 from goats and 30 from sheep. PCR assay was conducted using primers based on Theileria spp...
November 15, 2017: Parasitology International
https://www.readbyqxmd.com/read/29119682/molecular-identification-of-tick-borne-pathogens-infecting-cattle-in-mymensingh-district-of-bangladesh-reveals-emerging-species-of-anaplasma-and-babesia
#6
B C Roy, J Krücken, J S Ahmed, S Majumder, M P Baumann, P-H Clausen, A M Nijhof
Tick-borne diseases are considered a major hindrance to the health and productive performance of cattle in Bangladesh. To elucidate the epidemiology of tick-borne pathogens (TBPs) in local cattle, a cross-sectional study was performed in the 12 subdistricts (Upazilas) of Mymensingh district in Bangladesh. Blood samples and ticks were collected from 384 clinically healthy cattle kept by 135 farmers from 96 randomly selected villages. DNA extracted from the blood samples was subsequently screened by polymerase chain reaction (PCR) and a Reverse Line Blot (RLB) hybridization assay using an in-house prepared chemiluminescence solution for the presence of Anaplasma, Ehrlichia, Rickettsia, Babesia and Theileria spp...
November 8, 2017: Transboundary and Emerging Diseases
https://www.readbyqxmd.com/read/29114145/studies-on-theileriosis-in-goats-from-karnataka-south-india
#7
R Shruthi, P M Thimmareddy, G S Mamatha, B M Chandranaik, G C Puttalakshmamma
A study was conducted to determine the prevalence of theileriosis in goats from southern districts of Karnataka. Out of 47 goat blood samples examined by microscopy, 68.08% (32/47) were positive for Theileria spp. The parasitemia ranged from 1.0 to 1.8 and 0.1-0.9% in clinical and carrier animals respectively. Out of 325 ticks collected from goats, 92.6 (301) and 7.38 (24)% of the ticks were found to be Haemaphysalis and Rhipicephalus respectively. Hemaphysalis kutchensis (90.15%) was found to be the predominant species followed by R...
December 2017: Journal of Parasitic Diseases: Official Organ of the Indian Society for Parasitology
https://www.readbyqxmd.com/read/29103263/hematological-changes-associated-with-theileria-orientalis-infection-in-korean-indigenous-cattle
#8
Suhee Kim, Do-Hyeon Yu, Sung-Woo Kang, Jeong-Byoung Chae, Kyoung-Seong Choi, Hyeon-Cheol Kim, Bae-Keun Park, Joon-Seok Chae, Jinho Park
Tick-borne pathogens can cause serious problems in grazing cattle. However, little information is available on tick-mediated diseases in cattle grazing on mountains. Thus, this study aimed to understand the potential problems related to tick-borne diseases in grazing cattle through the investigation of prevalent tick-transmitted infections, and their associated hematological changes, in terms of season and grazing type in Korean indigenous cattle (=Hanwoo). Hanwoo cattle from 3 regions of the Republic of Korea (=Korea) were either maintained indoors or placed on grassy mountains from spring to fall of 2014 and 2015...
October 2017: Korean Journal of Parasitology
https://www.readbyqxmd.com/read/29081773/potential-sabotage-of-host-cell-physiology-by-apicomplexan-parasites-for-their-survival-benefits
#9
REVIEW
Shalini Chakraborty, Sonti Roy, Hiral Uday Mistry, Shweta Murthy, Neena George, Vasundhra Bhandari, Paresh Sharma
Plasmodium, Toxoplasma, Cryptosporidium, Babesia, and Theileria are the major apicomplexan parasites affecting humans or animals worldwide. These pathogens represent an excellent example of host manipulators who can overturn host signaling pathways for their survival. They infect different types of host cells and take charge of the host machinery to gain nutrients and prevent itself from host attack. The mechanisms by which these pathogens modulate the host signaling pathways are well studied for Plasmodium, Toxoplasma, Cryptosporidium, and Theileria, except for limited studies on Babesia...
2017: Frontiers in Immunology
https://www.readbyqxmd.com/read/29078748/whole-genome-assembly-of-babesia-ovata-and-comparative-genomics-between-closely-related-pathogens
#10
Junya Yamagishi, Masahito Asada, Hassan Hakimi, Takeshi Q Tanaka, Chihiro Sugimoto, Shin-Ichiro Kawazu
BACKGROUND: Babesia ovata, belonging to the phylum Apicomplexa, is an infectious parasite of bovids. It is not associated with the manifestation of severe symptoms, in contrast to other types of bovine babesiosis caused by B. bovis and B. bigemina; however, upon co-infection with Theileria orientalis, it occasionally induces exacerbated symptoms. Asymptomatic chronic infection in bovines is usually observed only for B. ovata. Comparative genomic analysis could potentially reveal factors involved in these distinguishing characteristics; however, the genomic and molecular basis of these phenotypes remains elusive, especially in B...
October 27, 2017: BMC Genomics
https://www.readbyqxmd.com/read/29066849/chemotherapeutic-efficacies-of-a-clofazimine-and-diminazene-aceturate-combination-against-piroplasm-parasites-and-their-at-rich-dna-binding-activity-on-babesia-bovis
#11
Bumduuren Tuvshintulga, Mahmoud AbouLaila, Thillaiampalam Sivakumar, Dickson Stuart Tayebwa, Sambuu Gantuya, Khandsuren Naranbaatar, Aki Ishiyama, Masato Iwatsuki, Kazuhiko Otoguro, Satoshi Ōmura, Mohamad Alaa Terkawi, Azirwan Guswanto, Mohamed Abdo Rizk, Naoaki Yokoyama, Ikuo Igarashi
Recently, we reported that clofazimine (CF) has an anti-piroplasm activity, but it could not completely eliminate parasites in the host. The currently available anti-piroplasm drug, diminazene aceturate (DA), has sometimes been reported to have toxic side effects. In the present study, we evaluated the combination treatment with CF and DA against piroplasms both in vitro and in vivo. Additionally, mRNA level and DNA amounts were analyzed in CF‒ and DA‒treated Babesia bovis by a qPCR. The CF-DA combination had additive effects on Babesia bovis, B...
October 24, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29056423/effect-of-recombinant-glutathione-s-transferase-as-vaccine-antigen-against-rhipicephalus-appendiculatus-and-rhipicephalus-sanguineus-infestation
#12
Gabriela Alves Sabadin, Luís Fernando Parizi, Irene Kiio, Marina Amaral Xavier, Renata da Silva Matos, Maria Izabel Camargo-Mathias, Naftaly Wang'ombe Githaka, Vish Nene, Itabajara da Silva Vaz
The ticks Rhipicephalus appendiculatus and Rhipicephalus sanguineus are the main vectors of Theileria parva and Babesia spp. in cattle and dogs, respectively. Due to their impact in veterinary care and industry, improved methods against R. appendiculatus and R. sanguineus parasitism are under development, including vaccines. We have previously demonstrated the induction of a cross-protective humoral response against Rhipicephalus microplus following vaccination with recombinant glutathione S-transferase from Haemaphysalis longicornis tick (rGST-Hl), suggesting that this protein could control tick infestations...
October 19, 2017: Vaccine
https://www.readbyqxmd.com/read/29035848/animal-level-risk-factors-associated-with-babesia-and-theileria-infections-in-cattle-in-egypt
#13
Mohamed Abdo Rizk, Akram Salama, Shimaa Abd-El-Salam El-Sayed, Ahmed Elsify, Maged El-Ashkar, Hussam Ibrahim, Mohamed Youssef, Sabry El-Khodery
In present study, blood samples were collected randomly from 439 cows at three main regions of Egypt (northern, central and southern). Molecular diagnosis of Babesia and Theileria infections by PCR amplification of DNA (gene) fragments, then cloning and sequencing of the positive samples were conducted. A questionnaire was created to imply the assumed risk factors and logistic regression statistical analysis was carried out to appraise the potential factors on the animal level. The results revealed that 49 (11...
December 20, 2017: Acta Parasitologica
https://www.readbyqxmd.com/read/29020888/clinical-haematology-and-biochemistry-profiles-of-cattle-naturally-infected-with-theileria-orientalis-ikeda-type-in-new-zealand
#14
K E Lawrence, S F Forsyth, B L Vaatstra, Amj McFadden, D J Pulford, K Govindaraju, W E Pomroy
AIMS: To present the haematology and biochemistry profiles for cattle in New Zealand naturally infected with Theileria orientalis Ikeda type and investigate if the results differed between adult dairy cattle and calves aged <6 months. METHODS: Haematology and biochemistry results were obtained from blood samples from cattle which tested positive for T. orientalis Ikeda type by PCR, that were submitted to veterinary laboratories in New Zealand between October 2012 and November 2014...
January 2018: New Zealand Veterinary Journal
https://www.readbyqxmd.com/read/28977247/genetic-diversity-of-piroplasmids-species-in-equids-from-island-of-s%C3%A3-o-lu%C3%A3-s-northeastern-brazil
#15
Maria do Socorro Costa de Oliveira Braga, Francisca Neide Costa, Débora Regina Maia Gomes, Daniele Rosa Xavier, Marcos Rogério André, Luiz Ricardo Gonçalves, Carla Roberta Freschi, Rosangela Zacarias Machado
Equine piroplasmosisis, a tick-borne disease caused by the intra-erythrocytic protozoans Babesia caballi and Theileria equi, has economic importance due to the international trade and the increased movement of horses all over the world. The goal of this study was to evaluate the occurrence of phylogenetic diversity of T. equi and B. caballi genotypes among infected equids from São Luís Island, state of Maranhão, northeastern Brazil. Between December of 2011 and June of 2012, EDTA-blood and serum samples were collected from 139 equids (90 donkeys, 39 horses and 10 mules)...
July 2017: Revista Brasileira de Parasitologia Veterinária, Brazilian Journal of Veterinary Parasitology
https://www.readbyqxmd.com/read/28969833/monitoring-theileria-orientalis-ikeda-associated-bovine-anaemia-in-affected-cattle-over-time
#16
Amj McFadden, M Hart, I M Bueno, H J Ha, Acg Heath, D J Pulford
The aim of the study was to observe changes in haematocrit (HCT) over time in a New Zealand South Island dairy herd affected by an outbreak of Theileria-associated bovine anaemia (TABA; Ikeda). A secondary aim was to relate individual cow HCTs to the amount of Theileria orientalis Ikeda DNA present in the blood, as measured by cycle threshold values, using a quantitative PCR (qPCR). Over a 6 month period, blood samples from 19 randomly selected cattle were monitored from a herd of 600 dairy cows. The sampling interval was approximately fortnightly for the first six weeks, followed by sampling at between four and six week intervals...
October 15, 2017: Veterinary Parasitology
https://www.readbyqxmd.com/read/28969779/seasonal-occurrence-of-theileria-parva-infection-and-management-practices-amongst-maasai-pastoralist-communities-in-monduli-district-northern-tanzania
#17
Esther G Kimaro, Siobhan M Mor, Paul Gwakisa, Jenny-Ann Toribio
Theileria parva causes an economically devastating tick-borne disease called East Coast fever (ECF), which affects cattle in central, eastern and southern Africa. Determination of seasonal infection rates for T. parva is crucial for epidemiological understanding and for strengthening ECF management practices. However, this information is lacking for most pastoralist areas with high livestock density, such as the Monduli District in the Maasai steppe, northern Tanzania. A cross-sectional study was carried out to estimate the prevalence of T...
November 15, 2017: Veterinary Parasitology
https://www.readbyqxmd.com/read/28959741/erratum-for-huber-et-al-the-microtubule-stabilizing-protein-clasp1-associates-with-the-theileria-annulata-schizont-surface-via-its-kinetochore-binding-domain
#18
Sandra Huber, Romina Theiler, Daniel de Quervain, Olga Wiens, Tulin Karagenc, Volker Heussler, Dirk Dobbelaere, Kerry Woods
[This corrects the article DOI: 10.1128/mSphere.00215-17.].
September 2017: MSphere
https://www.readbyqxmd.com/read/28958791/tick-borne-bacteria-and-protozoa-detected-in-ticks-collected-from-domestic-animals-and-wildlife-in-central-and-southern-portugal
#19
André Pereira, Ricardo Parreira, António José Cotão, Mónica Nunes, Maria Luísa Vieira, Fábia Azevedo, Lenea Campino, Carla Maia
Ticks are vectors of many human and animal pathogens. The aim of this study was to screen bacteria and protozoa from ticks infesting domestic animals and wildlife collected in central and southern Portugal. A total of 593 ticks, comprising 465 (78.4%) adults, 122 (20.6%) nymphs, and six (1.0%) larvae, were collected from 283 hosts of 25 different species (4 domestic and 21 wild). Overall, the analysis of DNA extracts prepared from ticks collected from hosts of 11 different species in the districts of Castelo Branco, Portalegre, Lisboa, Setúbal, Beja and Faro, revealed the presence of genomic sequences from Anaplasma sp...
September 20, 2017: Ticks and Tick-borne Diseases
https://www.readbyqxmd.com/read/28941935/diversity-of-piroplasmids-among-wild-and-domestic-mammals-and-ectoparasites-in-pantanal-wetland-brazil
#20
Keyla Carstens Marques de Sousa, Marina Pugnaghi Fernandes, Heitor Miraglia Herrera, Carla Roberta Freschi, Rosangela Zacarias Machado, Marcos Rogério André
Piroplasmoses are one of the most prevalent arthropod-borne diseases of animals. The present work aimed to investigate the occurrence of piroplasmid in wild mammals, domestic dogs and ectoparasites in southern Pantanal region, central-western Brazil. For that purpose, blood or tissue samples from 31 Nasua nasua, 78 Cerdocyon thous, 7 Leopardus pardalis, 42 dogs, 110 wild rodents, and 30 marsupials, and 1582 ticks were submitted to PCR assays for piroplasmid targeting 18SrRNA and hps70 genes. Seven dogs, one C...
September 20, 2017: Ticks and Tick-borne Diseases
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