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Mohamed Abdo Rizk, Akram Salama, Shimaa Abd-El-Salam El-Sayed, Ahmed Elsify, Maged El-Ashkar, Hussam Ibrahim, Mohamed Youssef, Sabry El-Khodery
In present study, blood samples were collected randomly from 439 cows at three main regions of Egypt (northern, central and southern). Molecular diagnosis of Babesia and Theileria infections by PCR amplification of DNA (gene) fragments, then cloning and sequencing of the positive samples were conducted. A questionnaire was created to imply the assumed risk factors and logistic regression statistical analysis was carried out to appraise the potential factors on the animal level. The results revealed that 49 (11...
December 20, 2017: Acta Parasitologica
K E Lawrence, S F Forsyth, B Vaatstra, A M J McFadden, D Pulford, K Govindaraju, W Pomroy
AIMS: To present the haematology and biochemistry profiles for cattle in New Zealand naturally infected with Theileria orientalis Ikeda type and investigate if the results differed between adult dairy cattle and calves aged <6 months. METHODS: Haematology and biochemistry results were obtained from blood samples from cattle which tested positive for T. orientalis Ikeda type by PCR, that were submitted to veterinary laboratories in New Zealand between October 2012 and November 2014...
October 11, 2017: New Zealand Veterinary Journal
Maria do Socorro Costa de Oliveira Braga, Francisca Neide Costa, Débora Regina Maia Gomes, Daniele Rosa Xavier, Marcos Rogério André, Luiz Ricardo Gonçalves, Carla Roberta Freschi, Rosangela Zacarias Machado
Equine piroplasmosisis, a tick-borne disease caused by the intra-erythrocytic protozoans Babesia caballi and Theileria equi, has economic importance due to the international trade and the increased movement of horses all over the world. The goal of this study was to evaluate the occurrence of phylogenetic diversity of T. equi and B. caballi genotypes among infected equids from São Luís Island, state of Maranhão, northeastern Brazil. Between December of 2011 and June of 2012, EDTA-blood and serum samples were collected from 139 equids (90 donkeys, 39 horses and 10 mules)...
July 2017: Revista Brasileira de Parasitologia Veterinária, Brazilian Journal of Veterinary Parasitology
Amj McFadden, M Hart, I M Bueno, H J Ha, Acg Heath, D J Pulford
The aim of the study was to observe changes in haematocrit (HCT) over time in a New Zealand South Island dairy herd affected by an outbreak of Theileria-associated bovine anaemia (TABA; Ikeda). A secondary aim was to relate individual cow HCTs to the amount of Theileria orientalis Ikeda DNA present in the blood, as measured by cycle threshold values, using a quantitative PCR (qPCR). Over a 6 month period, blood samples from 19 randomly selected cattle were monitored from a herd of 600 dairy cows. The sampling interval was approximately fortnightly for the first six weeks, followed by sampling at between four and six week intervals...
October 15, 2017: Veterinary Parasitology
Esther G Kimaro, Siobhan M Mor, Paul Gwakisa, Jenny-Ann Toribio
Theileria parva causes an economically devastating tick-borne disease called East Coast fever (ECF), which affects cattle in central, eastern and southern Africa. Determination of seasonal infection rates for T. parva is crucial for epidemiological understanding and for strengthening ECF management practices. However, this information is lacking for most pastoralist areas with high livestock density, such as the Monduli District in the Maasai steppe, northern Tanzania. A cross-sectional study was carried out to estimate the prevalence of T...
November 15, 2017: Veterinary Parasitology
Sandra Huber, Romina Theiler, Daniel de Quervain, Olga Wiens, Tulin Karagenc, Volker Heussler, Dirk Dobbelaere, Kerry Woods
[This corrects the article DOI: 10.1128/mSphere.00215-17.].
September 2017: MSphere
André Pereira, Ricardo Parreira, António José Cotão, Mónica Nunes, Maria Luísa Vieira, Fábia Azevedo, Lenea Campino, Carla Maia
Ticks are vectors of many human and animal pathogens. The aim of this study was to screen bacteria and protozoa from ticks infesting domestic animals and wildlife collected in central and southern Portugal. A total of 593 ticks, comprising 465 (78.4%) adults, 122 (20.6%) nymphs, and six (1.0%) larvae, were collected from 283 hosts of 25 different species (4 domestic and 21 wild). Overall, the analysis of DNA extracts prepared from ticks collected from hosts of 11 different species in the districts of Castelo Branco, Portalegre, Lisboa, Setúbal, Beja and Faro, revealed the presence of genomic sequences from Anaplasma sp...
September 20, 2017: Ticks and Tick-borne Diseases
Keyla Carstens Marques de Sousa, Marina Pugnaghi Fernandes, Heitor Miraglia Herrera, Carla Roberta Freschi, Rosangela Zacarias Machado, Marcos Rogério André
Piroplasmoses are one of the most prevalent arthropod-borne diseases of animals. The present work aimed to investigate the occurrence of piroplasmid in wild mammals, domestic dogs and ectoparasites in southern Pantanal region, central-western Brazil. For that purpose, blood or tissue samples from 31 Nasua nasua, 78 Cerdocyon thous, 7 Leopardus pardalis, 42 dogs, 110 wild rodents, and 30 marsupials, and 1582 ticks were submitted to PCR assays for piroplasmid targeting 18SrRNA and hps70 genes. Seven dogs, one C...
September 20, 2017: Ticks and Tick-borne Diseases
Jan Šlapeta, Stefan Saverimuttu, Larry Vogelnest, Cheryl Sangster, Frances Hulst, Karrie Rose, Paul Thompson, Richard Whittington
The short-beaked echidna (Tachyglossus aculeatus) and the platypus (Ornithorhynchus anatinus) are iconic egg-laying monotremes (Mammalia: Monotremata) from Australasia. The aim of this study was to demonstrate the utility of diversity profiles in disease investigations of monotremes. Using small subunit (18S) rDNA amplicon deep-sequencing we demonstrated the presence of apicomplexan parasites and confirmed by direct and cloned amplicon gene sequencing Theileria ornithorhynchi, Theileria tachyglossi, Eimeria echidnae and Cryptosporidium fayeri...
September 13, 2017: Infection, Genetics and Evolution
Malak Haidar, Perle Latré de Laté, Eileen J Kennedy, Gordon Langsley
One powerful application of cell penetrating peptides is the delivery into cells of molecules that function as specific competitors or inhibitors of protein-protein interactions. Ablating defined protein-protein interactions is a refined way to explore their contribution to a particular cellular phenotype in a given disease context. Cell-penetrating peptides can be synthetically constrained through various chemical modifications that stabilize a given structural fold with the potential to improve competitive binding to specific targets...
September 7, 2017: Bioorganic & Medicinal Chemistry
Yang Zhang, Bayin Chahan, Shifang Liu, Ruiqi Song, Yongchang Li, Huercha, Qingyong Guo, Hui Wu, Yutao Zhu
In order to found the epidemiological situation of T. equi in the horse herds in Ili Prefecture of Xinjiang Province, 723 blood samples collected from 4 counties and districts were test for T. equi through microscopic detection and Polymerase chain Reaction (PCR). In the result, we found that the 295 of 723 blood samples (40.8%) were positive for T. equi infection. The results showed that the choosed counties have a varying degrees infection. To our knowledge, this is the first time that we detected T. equi infection using the molecular techniques from Ili in Xinjiang Uygur Autonomous region...
September 15, 2017: Veterinary Parasitology
Peter O'Donoghue
A range of protistan parasites occur in the blood of vertebrates and are transmitted by haematophagous invertebrate vectors. Some 48 genera are recognized in bood primarily on the basis of parasite morphology and host specificity; including extracellular kinetoplastids (trypanosomatids) and intracellular apicomplexa (haemogregarines, haemococcidia, haemosporidia and piroplasms). Gene sequences are available for a growing number of species and molecular phylogenies often link parasite and host or vector evolution...
December 2017: International Journal for Parasitology. Parasites and Wildlife
C E Suarez, R P Bishop, H F Alzan, W A Poole, B M Cooke
Apicomplexan parasites such as Babesia, Theileria, Eimeria, Cryptosporidium and Toxoplasma greatly impact animal health globally, and improved, cost-effective measures to control them are urgently required. These parasites have complex multi-stage life cycles including obligate intracellular stages. Major gaps in our understanding of the biology of these relatively poorly characterised parasites and the diseases they cause severely limit options for designing novel control methods. Here we review potentially important shared aspects of the biology of these parasites, such as cell invasion, host cell modification, and asexual and sexual reproduction, and explore the potential of the application of relatively well-established or newly emerging genetic manipulation methods, such as classical transfection or gene editing, respectively, for closing important gaps in our knowledge of the function of specific genes and proteins, and the biology of these parasites...
October 2017: International Journal for Parasitology
Munir Aktas, Sezayi Ozubek
Hemotropic mycoplasmas are emerging zoonotic pathogens that cause mild to severe hemolytic anemia. Blood samples and thin blood smears were collected from six sheep exhibiting clinical symptoms typical of hemolytic anemia. Coccoid and ring-shaped M. ovis were found on the surface of erythrocytes of infected sheep. Parasitemia ranged from 0.7% to 3.7% in individual smears. Clinical cases caused by Mycoplasma ovis were evaluated, and prevalence, based on DNA screening for the 16S rRNA gene of hemoplasmas in apparently healthy sheep (n=328) and goats (n=272) from the same area, was 11...
September 2017: Veterinary Microbiology
Lydia Calleja-Bueno, Ángel Sainz, Mercedes García-Sancho, Fernando Rodríguez-Franco, Juan V González-Martín, Alejandra Villaescusa
Mediterranean theileriosis is one of the most fatal theilerioses, with considerable economic impact on livestock production. The potential consequences of asymptomatic infection on the animal's health and on the epidemiology in endemic regions are still unclear. The objectives of this study were to determine the molecular prevalence of T. annulata in a representative population of asymptomatic cattle in extensive management in Madrid, Central Spain, an area where practitioners frequently report cases of clinical theileriosis, and to evaluate the existence of associations between infection by this pathogen and haematological, biochemical and epidemiological data...
August 25, 2017: Ticks and Tick-borne Diseases
L N Wise, L S Kappmeyer, M G Silva, S N White, J F Grause, D P Knowles
Certain countries including the United States remain non-endemic for particular infectious diseases such as equine piroplasmosis through import restrictions and surveillance. Endemic regions often employ premunition as the primary method to control disease, however in non-endemic countries, chemosterilization combined with methods to confirm parasite elimination are required to maintain disease-free status. The ability of imidocarb diproprionate (ID) to clear persistent Theileria equi infection from infected horses has been shown through the inability of treated horses to transmit via blood transfer...
August 25, 2017: Ticks and Tick-borne Diseases
Lan He, Xiaoyan Miao, Jinfang Hu, Yuan Huang, Pei He, Junwei He, Long Yu, Ngabu Malobi, Ligang Shi, Junlong Zhao
Canine piroplasmosis is a significant disease in dogs caused by Babesia and Theileria parasites. The clinical manifestations range from mild illness to serious disease depending on the parasite species and the physical condition of the infected dog. Canine piroplasmosis has been reported to be prevalent in China. However, no molecular evidence of the disease has been reported in pet dogs from Wuhan. In this study, 118 blood samples were randomly collected from pet dogs in veterinary clinics. The blood samples were subjected to both microscopic examination and reverse line blot (RLB) hybridization assays to detect piroplasm infection...
2017: Frontiers in Microbiology
(no author information available yet)
No abstract text is available yet for this article.
September 4, 2017: New Zealand Veterinary Journal
Sandra Huber, Romina Theiler, Daniel de Quervain, Olga Wiens, Tulin Karangenc, Volker Heussler, Dirk Dobbelaere, Kerry Woods
Theileria is an apicomplexan parasite whose presence within the cytoplasm of a leukocyte induces cellular transformation and causes uncontrolled proliferation and clonal expansion of the infected cell. The intracellular schizont utilizes the host cell's own mitotic machinery to ensure its distribution to both daughter cells by associating closely with microtubules (MTs) and incorporating itself within the central spindle. We show that CLASP1, an MT-stabilizing protein that plays important roles in regulating kinetochore-MT attachment and central spindle positioning, is sequestered at the Theileria annulata schizont surface...
July 2017: MSphere
Yanyan Cui, Xiaoxing Wang, Yan Zhang, Yaqun Yan, Haiju Dong, Fuchun Jian, Ke Shi, Longxian Zhang, Rongjun Wang, Changshen Ning
Tick-borne diseases (TBDs) impose a significant constraint to livestock production world widely. In this paper, we presented a case of TBD in a cattle farm in Henan, China. 35 blood samples (7 samples sent by veterinarian, 28 samples gathered by our colleagues) were collected from ill, surviving and asymptomatic cattle and microscopic observation and PCR assays were conducted to characterize the pathogens. Genus Ixodes feeding on these cattle were collected and identified. Theileria annulata-like and Anaplasma marginale-like pathogens were observed in the blood smears stained with Giemsa staining under microscope...
August 28, 2017: Acta Tropica
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