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https://www.readbyqxmd.com/read/28188085/species-of-ticks-and-carried-pathogens-in-owned-dogs-in-spain-results-of-a-one-year-national-survey
#1
Agustín Estrada-Peña, Xavier Roura, Angel Sainz, Guadalupe Miró, Laia Solano-Gallego
This study presents the results of a national survey in Spain on the distribution of ticks on owned dogs, their phenology and the associated pathogens over one year. In the study, 1628 adult ticks were collected on 660 dogs presented to 26 veterinary practices, of which 507 dogs (76.8%) carried at least one adult tick. The primary species of ticks were Rhipicephalus sanguineus s.l. (53%), Dermacentor reticulatus (9%), Ixodes ricinus (9%), and I. hexagonus (4%). Parasitism by two species of these ticks was rare...
February 3, 2017: Ticks and Tick-borne Diseases
https://www.readbyqxmd.com/read/28169066/standardization-and-validation-of-simple-pcr-duplex-pcr-and-rapd-in-comparison-to-blood-smear-examination-for-diagnosing-bovine-tropical-theileriosis
#2
Vikrant Sudan, Daya Shanker, Amit Jaiswal, Amit Singh, Vijay Pandey
Bovine Tropical Theileriosis (BTT) is an important vector-borne protozoan disease that imposing serious constraints on the health and productivity of domestic cattle. It is matter of common fact that following recovery from primary infection, cattle become persistent carriers and act as reservoirs of infection thereby, playing a critical role in disease epidemiology. The present study describes the comparative diagnostic efficiency of simplex PCR, duplex PCR and RAPD assays for detection of Theileria annulata in cattle...
February 3, 2017: Biologicals: Journal of the International Association of Biological Standardization
https://www.readbyqxmd.com/read/28155040/-what-makes-a-parasite-transforming-insights-into-cancer-from-the-agents-of-an-exotic-pathology-theileria-spp
#3
K M Cheeseman, J B Weitzman
Theileria are obligate eukaryotic intracellular parasites of cattle. The diseases they cause, Tropical theileriosis and East Coast Fever, cause huge economic loss in East African, Mediterranean and central and South-East Asian countries. These apicomplexan parasites are the only intracellular eukaryotic parasites known to transform their host cell and represent a unique model to study host-parasite interactions and mechanisms of cancer onset. Here, we review how Theileria parasites induce transformation of their leukocyte host cell and discuss similarities with tumorigenesis...
February 2, 2017: Bulletin de la Société de Pathologie Exotique
https://www.readbyqxmd.com/read/28153803/development-of-duplex-pcr-for-simultaneous-detection-of-theileria-spp-and-anaplasma-spp-in-sheep-and-goats
#4
Yanyan Cui, Yan Zhang, Fuchun Jian, Longxian Zhang, Rongjun Wang, Shuxuan Cao, Xiaoxing Wang, Yaqun Yan, Changshen Ning
Theileria spp. and Anaplasma spp., which are important tick-borne pathogens (TBPs), impact the health of humans and animals in tropical and subtropical areas. Theileria and Anaplasma co-infections are common in sheep and goats. Following alignment of the relevant DNA sequences, two primer sets were designed to specifically target the Theileria spp. 18S rRNA and Anaplasma spp. 16S rRNA gene sequences. Genomic DNA from the two genera was serially diluted tenfold for testing the sensitivities of detection of the primer sets...
January 30, 2017: Experimental Parasitology
https://www.readbyqxmd.com/read/28151085/natural-infection-of-the-south-american-tapir-tapirus-terrestris-by-theileria-equi
#5
Alexandre Welzel Da Silveira, Gustavo Gomes De Oliveira, Leandro Menezes Santos, Lucas Bezerra da Silva Azuaga, Claudia Regina Macedo Coutinho, Jessica Teles Echeverria, Tamires Ramborger Antunes, Carlos Alberto do Nascimento Ramos, Alda Izabel de Souza
Theileria equi is a tick-borne piroplasm considered endemic in equines in Brazil. The cohabitation of domestic and wild animals in areas of extensive cattle breeding favors the close contact between different species and the sharing of vectors and, consequently, pathogens. We report the natural infection of a young South American tapir ( Tapirus terrestris ) by Theileria equi in Mato Grosso do Sul, Brazil. Although it was not possible to associate the clinical and hematologic status of the animal with the infection by the protozoan parasite, our report represents an alert on the sharing of pathogens between domestic and wild animals...
February 2, 2017: Journal of Wildlife Diseases
https://www.readbyqxmd.com/read/28131376/molecular-evidence-of-tick-borne-hemoprotozoan-parasites-theileria-ovis-and-babesia-ovis-and-bacteria-in-ticks-and-blood-from-small-ruminants-in-northern-algeria
#6
Atef Aouadi, Hamza Leulmi, Mehdi Boucheikhchoukh, Ahmed Benakhla, Didier Raoult, Philippe Parola
Using qPCR, standard PCR and/or sequencing, we investigated the presence of tick-associated microorganisms in ticks and blood from sheep and goats from Souk Ahras, Algeria. Borrelia theileri, was detected in (7/120, 5.8%) blood from sheep and (13/120, 10.8%) goats. Anaplasma ovis was screened in (38/73, 52%) Rhipicephalus bursa and (5/22, 22.7%) R. turanicus and in (74/120, 61.7%), (65/120, 54.2%) blood of sheep and goats respectively. Coxiella burnetii tested positive in R. bursa (4/73, 5.5%) and (7/120, 5...
February 2017: Comparative Immunology, Microbiology and Infectious Diseases
https://www.readbyqxmd.com/read/28127369/molecular-identification-of-hemoprotozoan-parasites-in-camels-camelus-dromedarius-of-iran
#7
Alireza Sazmand, Barbara Eigner, Mohammad Mirzaei, Seyed Hossein Hekmatimoghaddam, Josef Harl, Georg Gerhard Duscher, Hans-Peter Fuehrer, Anja Joachim
BACKGROUND: Although camels represent a valuable source of food, wool and hide in many countries, in-depth information about their vector-borne pathogens is scarce compared to other animals. The aim of the current study was to characterize vector-borne protozoa in the blood of dromedaries from Iran by molecular tools. METHODS: From June to July 2014, 200 peripheral blood samples were collected from asymptomatic one-humped camels in two provinces of Kerman and Sistan- va-Baloochestan in central and southeastern Iran...
October 2016: Iranian Journal of Parasitology
https://www.readbyqxmd.com/read/28118556/investigation-into-individual-health-and-exposure-to-infectious-agents-of-platypuses-ornithorhynchus-anatinus-in-two-river-catchments-in-northwest-tasmania
#8
James W Macgregor, Carly S Holyoake, Sarah A Munks, Joanne H Connolly, Ian D Robertson, Patricia A Fleming, Kristin S Warren
Changes in the health of individuals within wildlife populations can be a cause or effect of population declines in wildlife species. Aspects of individual platypus ( Ornithorhynchus anatinus ) health have been reported. However, holistic studies investigating potential synergistic effects of both pathogens and environmental factors are needed to expand understanding of platypus individual health. We collected baseline data on the health of platypuses in two Tasmanian river catchments (including evidence of the potentially fatal fungal disease mucormycosis) and on individual, demographic, geographic, and temporal patterns associated with health data results...
January 24, 2017: Journal of Wildlife Diseases
https://www.readbyqxmd.com/read/28118097/a-loop-mediated-isothermal-amplification-lamp-assay-targeting-16s-rrna-gene-for-rapid-detection-of-anaplasma-phagocytophilum-infection-in-sheep-and-goats
#9
Changshen Ning, Jinhong Wang, Yan Zhang, Xiaoxing Wang, Yanyan Cui, Yaqun Yan, Rongjun Wang, Fuchun Jian, Longxian Zhang
Anaplasma phagocytophilum is a zoonotic pathogen and the causative agent of human granulocytic anaplasmosis (HGA) in humans and tick-borne fever in various kinds of animals. In the present study, a loop-mediated isothermal amplification (LAMP) assay for rapid detection of A. phagocytophilum was developed using primers specific to 16S rRNA gene of this organism. The LAMP assay was performed at 65 C for 60 min and terminated at 80 C for 10 min. The optimal reaction conditions, under which no cross-reaction was observed with other closely related tick borne parasites (Anaplasma bovis, Anaplasma ovis, Theileria luwenshuni, Babesia motasi and Schistosoma japonicum) was established...
January 24, 2017: Journal of Parasitology
https://www.readbyqxmd.com/read/28116531/dermacentor-reticulatus-a-putative-vector-of-babesia-cf-microti-syn-theileria-annae-piroplasm
#10
Adnan Hodžić, Johanna Zörer, Georg Gerhard Duscher
Babesia cf. microti (syn. Theileria annae, Babesia microti-like, Babesia vulpes) is a recently recognized tick-borne piroplasm that infects domestic and wild carnivores. Although Ixodes hexagonus is considered as the leading candidate responsible for the transmission, its capacity to act as a competent vector has not yet been confirmed. This study reports the occurrence of B.cf. microti in unfed Dermacentor reticulatus for the first time, suggesting that this tick species may be implicated in the life cycle of this canine parasite...
January 23, 2017: Parasitology Research
https://www.readbyqxmd.com/read/28103801/parasite-infection-of-public-databases-a-data-mining-approach-to-identify-apicomplexan-contaminations-in-animal-genome-and-transcriptome-assemblies
#11
Janus Borner, Thorsten Burmester
BACKGROUND: Contaminations from various exogenous sources are a common problem in next-generation sequencing. Another possible source of contaminating DNA are endogenous parasites. On the one hand, undiscovered contaminations of animal sequence assemblies may lead to erroneous interpretation of data; on the other hand, when identified, parasite-derived sequences may provide a valuable source of information. RESULTS: Here we show that sequences deriving from apicomplexan parasites can be found in many animal genome and transcriptome projects, which in most cases derived from an infection of the sequenced host specimen...
January 19, 2017: BMC Genomics
https://www.readbyqxmd.com/read/28096609/studies-on-alterations-of-clinical-and-hemato-biochemical-parameters-before-and-after-treatment-in-calves-naturally-infected-with-theileriosis
#12
J P Kachhawa, Surender Kumar, Ankita Sharma, A P Singh, Anil Ahuja
AIM: The aim was to determine hemato-biochemical alterations and to determine the better treatment of theileriosis in naturally infected calves. MATERIALS AND METHODS: A total of 74 Holstein crossbred calves below 6 months of age, of either sex were included for present investigation in Bikaner. Based on the clinical examinations and laboratory results, 20 calves included for hemato-biochemical studies (before and after treatment) and divided into two groups (having 10 calves each)...
December 2016: Veterinary World
https://www.readbyqxmd.com/read/28055573/guidelines-for-the-detection-of-babesia-and-theileria-parasites
#13
Laetitia Lempereur, Relja Beck, Isabel Fonseca, Cátia Marques, Ana Duarte, Marcos Santos, Sara Zúquete, Jacinto Gomes, Gernot Walder, Ana Domingos, Sandra Antunes, Gad Baneth, Cornelia Silaghi, Patricia Holman, Annetta Zintl
The genera Babesia and Theileria (phylum Apicomplexa, order Piroplasmida) are mainly transmitted by Ixodid ticks in which the sexual part of their life cycle followed by sporogony takes place. They include protozoan parasites that infect erythrocytes of a variety of vertebrate hosts, including domestic and wild animals, with some Babesia spp. also infecting humans. Babesia sporozoites transmitted in the tick's saliva during the bloodmeal directly infect erythrocytes, where they asexually multiply to produce pear-shaped merozoites in the process of merogony; whereas a pre-erythrocytic schizogonic life stage in leukocytes is found in Theileria and precedes merogony in the erythrocytes...
January 2017: Vector Borne and Zoonotic Diseases
https://www.readbyqxmd.com/read/28043378/molecular-evidence-of-a-new-babesia-sp-in-goats
#14
Sezayi Ozubek, Munir Aktas
In this study, a novel Babesia sp. infecting goats was detected and its phylogenetic relationship to related species was determined. A total of 200 blood samples collected from sheep (n=78) and goats (n=122) were examined in the study. The V4 hypervariable region of the 18S rRNA gene of the novel Babesia sp. was amplified by PCR and analysed using a reverse line blot hybridization assay adapted for small ruminants. Samples from seven goats hybridized to Theileria/Babesia catch-all and Babesia catch-all probes and did not hybridize to any species-specific probe tested, suggesting the presence of an unrecognized Babesia species or genotype...
January 15, 2017: Veterinary Parasitology
https://www.readbyqxmd.com/read/28030350/prevalence-and-molecular-characterization-of-theileria-equi-and-babesia-caballi-in-jereed-horses-in-erzurum-turkey
#15
Esin Guven, Hamza Avcioglu, Ahmet Deniz, İbrahim Balkaya, Ugur Abay, Şevki Yavuz, Muzaffer Akyüz
Equine piroplasmosis (EP) is a hemoprotozoan tick-borne disease with worldwide distribution that is caused by Theileria equi and Babesia caballi. There are studies reporting the presence of equine piroplasmosis in Turkey but the situation in Erzurum is unknown. The aim of the current study was to determine the situation of equine piroplasmosis in jeered horses in Erzurum. Between April and August 2015, a total of 125 Arabian horse were examined and blood samples were collected. At the time of sampling, animals were also examined for tick infestations and clinical signs...
March 1, 2017: Acta Parasitologica
https://www.readbyqxmd.com/read/28028631/molecular-detection-and-genetic-diversity-of-bovine-babesia-spp-theileria-orientalis-and-anaplasma-marginale-in-beef-cattle-in-thailand
#16
Charoonluk Jirapattharasate, Paul Franck Adjou Moumouni, Shinuo Cao, Aiko Iguchi, Mingming Liu, Guanbo Wang, Mo Zhou, Patrick Vudriko, Artemis Efstratiou, Tanasak Changbunjong, Sivapong Sungpradit, Parntep Ratanakorn, Walasinee Moonarmart, Poonyapat Sedwisai, Thekhawet Weluwanarak, Witsanu Wongsawang, Hiroshi Suzuki, Xuenan Xuan
Babesia spp., Theileria orientalis, and Anaplasma marginale are significant tick-borne pathogens that affect the health and productivity of cattle in tropical and subtropical areas. In this study, we used PCR to detect the presence of Babesia bovis, Babesia bigemina, and T. orientalis in 279 beef cattle from Western Thailand and A. marginale in 608 beef cattle from the north, northeastern, and western regions. The PCRs were performed using species-specific primers based on the B. bovis spherical body protein 2 (BboSBP2), B...
February 2017: Parasitology Research
https://www.readbyqxmd.com/read/28013043/a-field-evaluation-of-an-isothermal-dna-amplification-assay-for-the-detection-of-theileria-annulata-infection-in-cattle
#17
Jacinto Gomes, Marcos Santos, Ana Amaro, Isabel Pereira da Fonseca, Gabriela Santos-Gomes, João Inácio
A loop-mediated isothermal amplification (LAMP) assay was evaluated for the detection of Theileria annulata infection in cattle. The results were compared with a real-time PCR used for the quantification of T. annulata parasitaemia. One hundred bovine blood samples from 16 cattle farms were tested with LAMP and real-time PCR, with T. annulata DNA being detected in 66% and 67% of the samples, respectively. The results showed that the LAMP assay detects a parasitaemia as low as 0.00025%, indicating a high analytical sensitivity of LAMP for clinical diagnosis of bovine theileriosis...
December 22, 2016: Molecular and Cellular Probes
https://www.readbyqxmd.com/read/28011185/novel-rickettsia-and-emergent-tick-borne-pathogens-a-molecular-survey-of-ticks-and-tick-borne-pathogens-in-shimba-hills-national-reserve-kenya
#18
Micky M Mwamuye, Edward Kariuki, David Omondi, James Kabii, David Odongo, Daniel Masiga, Jandouwe Villinger
Ticks are important vectors of emerging and re-emerging zoonoses, the majority of which originate from wildlife. In recent times, this has become a global public health concern that necessitates surveillance of both known and unknown tick-borne pathogens likely to be future disease threats, as well as their tick vectors. We carried out a survey of the diversity of ticks and tick-borne pathogens in Kenya's Shimba Hills National Reserve (SHNR), an area with intensified human-livestock-wildlife interactions, where we collected 4297 questing ticks (209 adult ticks, 586 nymphs and 3502 larvae)...
September 5, 2016: Ticks and Tick-borne Diseases
https://www.readbyqxmd.com/read/27986546/molecular-epidemiological-survey-of-bacterial-and-parasitic-pathogens-in-hard-ticks-from-eastern-china
#19
Xiang-Ye Liu, Xiang-Yao Gong, Chen Zheng, Qi-Yuan Song, Ting Chen, Jing Wang, Jie Zheng, Hong-Kuan Deng, Kui-Yang Zheng
Ticks are able to transmit various pathogens-viruses, bacteria, and parasites-to their host during feeding. Several molecular epidemiological surveys have been performed to evaluate the risk of tick-borne pathogens in China, but little is known about pathogens circulating in ticks from eastern China. Therefore, this study aimed to investigate the presence of bacteria and parasites in ticks collected from Xuzhou, a 11258km(2) region in eastern China. In the present study, ticks were collected from domestic goats and grasses in urban districts of Xuzhou region from June 2015 to July 2016...
March 2017: Acta Tropica
https://www.readbyqxmd.com/read/27942962/evaluating-an-indirect-rmpsp-enzyme-linked-immunosorbent-assay-for-the-detection-of-bovine-theileria-infection-in-china
#20
Shuaiyang Zhao, Junlong Liu, Hongxi Zhao, Youquan Li, Junren Xie, Aihong Liu, Muhammad-Adeel Hassan, Hong Yin, Guiquan Guan, Jianxun Luo
Bovine theileriosis, a tick-borne protozoan disease caused by Theileria annulata, Theileria orientalis and Theileria sinensis, is widespread in China and is a serious economic problem for the Chinese livestock industry. In this study, recombinant major piroplasma surface proteins (MPSP) of T. annulata, T. orientalis and T. sinensis based on MPSP genes were expressed in Escherichia coli BL21(DE3). The immunogenicity and specificity of the three purified recombinant MPSP proteins were evaluated with the reference positive sera of T...
February 2017: Parasitology Research
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