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https://www.readbyqxmd.com/read/28532427/microrna-275-and-its-target-vitellogenin-2-are-crucial-in-ovary-development-and-blood-digestion-of-haemaphysalis-longicornis
#1
Jiawei Hao, Jin Luo, Ze Chen, Qiaoyun Ren, Jinxia Guo, Xiaocui Liu, Qiuyu Chen, Feng Wu, Zhen Wang, Jianxun Luo, Hong Yin, Hui Wang, Guangyuan Liu
BACKGROUND: The hard tick Haemaphysalis longicornis is widely distributed in eastern Asia, New Zealand and Australia and is considered the major vector of Theileria and Babesia, harmful parasites to humans and animals. Female ticks need successful blood meals to complete the life-cycle. Therefore, elucidation of the underlying molecular mechanisms of H. longicornis development and reproduction is considered important for developing control strategies against the tick and tick-borne pathogens...
May 22, 2017: Parasites & Vectors
https://www.readbyqxmd.com/read/28502642/molecular-detection-of-tick-borne-pathogens-in-wild-red-foxes-vulpes-vulpes-from-central-italy
#2
Valentina Virginia Ebani, Guido Rocchigiani, Simona Nardoni, Fabrizio Bertelloni, Violetta Vasta, Roberto Amerigo Papini, Ranieri Verin, Alessandro Poli, Francesca Mancianti
Spleen samples from 153 red foxes, shot during regular hunting season in the province of Pisa (Central Italy), were examined to detect DNA of Anaplasma phagocytophilum, Ehrlichia canis, Coxiella burnetii, Francisella tularensis, Hepatozoon canis and Babesia sp./Theileria sp. DNA of vector-borne pathogens was detected in 120 (78.43%; 95% CI: 71.06-84.66%) foxes. Specifically, 75 (49%; 95% CI: 40.86-57.22%) animals scored PCR-positive per H. canis, 68 (44.44%; 95% CI: 36.42-52.69%) for E. canis, 35 (22.88%; 95% CI: 16...
May 11, 2017: Acta Tropica
https://www.readbyqxmd.com/read/28502276/characterization-of-tabanid-flies-diptera-tabanidae-in-south-africa-and-zambia-and-detection-of-protozoan-parasites-they-are-harbouring
#3
Moeti O Taioe, Makhosazana Y Motloang, Boniface Namangala, Amos Chota, Nthatisi I Molefe, Simon P Musinguzi, Keisuke Suganuma, Polly Hayes, Toi J Tsilo, John Chainey, Noboru Inoue, Oriel M M Thekisoe
Tabanids are haematophagous flies feeding on livestock and wildlife. In the absence of information on the relationship of tabanid flies and protozoan parasites in South Africa and Zambia, the current study was aimed at characterizing tabanid flies collected in these two countries as well as detecting protozoan parasites they are harbouring. A total of 527 tabanid flies were collected whereby 70·2% were from South Africa and 29·8% were from Zambia. Morphological analysis revealed a total of five different genera collected from the sampled areas namely: Ancala, Atylotus, Haematopota, Philoliche and Tabanus...
May 15, 2017: Parasitology
https://www.readbyqxmd.com/read/28501503/detection-and-molecular-characterization-of-babesia-theileria-and-hepatozoon-species-in-hard-ticks-collected-from-kagoshima-the-southern-region-in-japan
#4
Tatsunori Masatani, Kei Hayashi, Masako Andoh, Morihiro Tateno, Yasuyuki Endo, Masahito Asada, Kodai Kusakisako, Tetsuya Tanaka, Mutsuyo Gokuden, Nodoka Hozumi, Fumiko Nakadohzono, Tomohide Matsuo
To reveal the distribution of tick-borne parasites, we established a novel nested polymerase chain reaction (PCR) system to detect the most common agents of tick-borne parasitic diseases, namely Babesia, Theileria, and Hepatozoon parasites. We collected host-seeking or animal-feeding ticks in Kagoshima Prefecture, the southernmost region of Kyusyu Island in southwestern Japan. Twenty of the total of 776 tick samples displayed a specific band of the appropriate size (approximately 1.4-1.6kbp) for the 18S rRNA genes in the novel nested PCR (20/776: 2...
March 27, 2017: Ticks and Tick-borne Diseases
https://www.readbyqxmd.com/read/28497743/seroprevalence-of-theileria-equi-and-babesia-caballi-in-horses-in-spain
#5
Maria Guadalupe Montes Cortés, José Luis Fernández-García, Miguel Ángel Habela Martínez-Estéllez
Equine piroplasmoses are enzootic parasitic diseases distributed worldwide with high incidence in tropical and subtropical regions. In Spain, there is insufficient epidemiological data about equine piroplasmoses. The main aim of the present study was therefore to estimate the prevalence of Theileria equi and Babesia caballi in five regions and obtain information about the risk factors. This study was conducted in the central and south-western regions of Spain, using indirect fluorescence antibody testing (IFAT) in 3,100 sera samples from apparently healthy horses of different ages, breeds, coat colours, genders and geographical locations...
2017: Parasite: Journal de la Société Française de Parasitologie
https://www.readbyqxmd.com/read/28495200/genotyping-of-theileria-lestoquardi-from-sheep-and-goats-in-sudan-to-support-control-of-malignant-ovine-theileriosis
#6
A M Ali, D A Salih, M N Njahira, S K Hassan, A M El Hussein, Z Liu, H Yin, R Pelle, R A Skilton
Theileriosis, caused by parasitic protozoa of the genus Theileria parasites, are among the major tick-borne diseases of ruminant livestock. The largest economic losses are attributed in particular to those caused by the leukoproliferative species of Theileria: T. parva, T. annulata and T. lestoquardi. Theileria lestoquardi is transmitted by Hyalomma ticks and causes malignant ovine theileriosis (MOT), a disease that is particularly prevalent in Sudan. The disease is considered of a high economic importance in Sudan, where export of sheep is a major component of the national economy...
May 30, 2017: Veterinary Parasitology
https://www.readbyqxmd.com/read/28483728/selection-of-genetic-markers-to-determine-diversity-in-theileria-annulata-populations-after-recombination
#7
Hüseyin Bilgin Bilgiç, Ahmet Hakan Ünlü, Ayça Aksulu, Serkan Bakırcı, Selin Hacılarlıoğlu, Hasan Eren, William Weir, Tülin Karagenç
OBJECTIVE: Selecting polymorphic mini- and microsatellite markers to determine genetic diversity and chromosomal regions exhibiting elevated rates of recombination in Theileria annulata populations after recombination. METHODS: The Unipro UGENE software was used to select markers. A score at which 10 times more tandem repeats (TRs) were identified in the real DNA sequence than those in the scrambled sequences of T. annulata was used as the cutoff. TRs containing minimum three nucleotides in length for microsatellite and six nucleotides for minisatellite regions and having a repeat motif identity between 96%-100% with the unit size repeated minimum three times were screened through the whole genome using the suffix array algorithm...
March 2017: Türkiye Parazitolojii Dergisi
https://www.readbyqxmd.com/read/28482923/transplacental-transmission-of-theileria-orientalis-occurs-at-a-low-rate-in-field-affected-cattle-infection-in-utero-does-not-appear-to-be-a-major-cause-of-abortion
#8
Emma Swilks, Shayne A Fell, Jade F Hammer, Narelle Sales, Gaye L Krebs, Cheryl Jenkins
BACKGROUND: Bovine theileriosis, caused by the haemoprotozoan Theileria orientalis, is an emerging disease in East Asia and Australasia. Previous studies have demonstrated transplacental transmission of various Theileria spp. but molecular confirmation of transplacental transmission of T. orientalis has never been confirmed in the field. In this study, cow-calf (< 48 h old) pairs were sampled across 3 herds; opportunistic samples from aborted foetuses or stillborn calves were also examined...
May 8, 2017: Parasites & Vectors
https://www.readbyqxmd.com/read/28449722/prevalence-of-tick-borne-haemoparasites-in-small-ruminants-in-turkey-and-diagnostic-sensitivity-of-single-pcr-and-rlb
#9
Huseyin Bilgin Bilgic, Serkan Bakırcı, Onur Kose, Ahmet Hakan Unlu, Selin Hacılarlıoglu, Hasan Eren, William Weir, Tulin Karagenc
BACKGROUND: Tick-borne haemoparasitic diseases (TBHDs), caused by Theileria, Babesia, Anaplasma and Ehrlichia, are common in regions of the world where the distributions of host, pathogen and vector overlap. Many of these diseases threaten livestock production and some also represent a concern to human public health. The primary aim of this study was to determine the prevalence of the above-mentioned pathogens in a large number of blood samples (n = 1979) collected from sheep (n = 1727) and goats (n = 252) in Turkey...
April 27, 2017: Parasites & Vectors
https://www.readbyqxmd.com/read/28427963/molecular-investigation-of-piroplasma-infection-in-white-yaks-bos-grunniens-in-gansu-province-china
#10
Sitong Li, Junlong Liu, Aihong Liu, Youquan Li, Shuqing Wang, Shufang Wang, Hong Yin, Jianxun Luo, Guiquan Guan
Piroplasmosis, including theileriosis and babesiosis, is a tick-borne haemoprotozoan disease responsible for huge economic losses to livestock industry. In China, the biology of piroplasms infective to cattle was well understood on the basis of pathogen isolations and molecular epidemiological surveys in the past few decades. But very limited information about the infection status of piroplasms in white yak (Bos grunniens), a semi-wild and endemic breed, has been recorded, so far. A total of 350 blood samples was collected from white yaks in 11 towns of Tianzhu Tibetan Autonomous County of Gansu province, China, during April to July 2015...
April 17, 2017: Acta Tropica
https://www.readbyqxmd.com/read/28416183/inhibition-of-the-in-vitro-growth-of-babesia-bigemina-babesia-caballi-and-theileria-equi-parasites-by-trifluralin-analogues
#11
Marta G Silva, Donald P Knowles, Sandra Antunes, Ana Domingos, Maria A Esteves, Carlos E Suarez
Bovine and equine babesiosis caused by Babesia bovis, Babesia bigemina and Babesia caballi, along with equine theileriosis caused by Theileria equi are global tick-borne hemoprotozoan diseases characterized by fever, anemia, weight losses and abortions. A common feature of these diseases are transition from acute to chronic phases, in which parasites may persist in the hosts for life. Antiprotozoal drugs are important for managing infection and disease. Previous research demonstrated that trifluralin analogues, designated (TFLAs) 1-15, which specifically bind to regions of alpha-tubulin protein in plants and protozoan parasites, have the ability to inhibit the in vitro growth of B...
April 10, 2017: Ticks and Tick-borne Diseases
https://www.readbyqxmd.com/read/28411879/apicomplexan-autophagy-and-modulation-of-autophagy-in-parasite-infected-host-cells
#12
REVIEW
Perle Laté de Laté, Miguel Pineda, Margaret Harnett, William Harnett, Sébastien Besteiro, Gordon Langsley
Apicomplexan parasites are responsible for a number of important human pathologies. Obviously, as Eukaryotes they share a number of cellular features and pathways with their respective host cells. One of them is autophagy, a process involved in the degradation of the cell's own components. These intracellular parasites nonetheless seem to present a number of original features compared to their very evolutionarily distant host cells. In mammals and other metazoans, autophagy has been identified as an important contributor to the defence against microbial pathogens...
February 2017: Biomedical Journal
https://www.readbyqxmd.com/read/28410591/eurasian-golden-jackal-as-host-of-canine-vector-borne-protists
#13
Barbora Mitková, Kristýna Hrazdilová, Gianluca D'Amico, Georg Gerhard Duscher, Franz Suchentrunk, Pavel Forejtek, Călin Mircea Gherman, Ioana Adriana Matei, Angela Monica Ionică, Aikaterini Alexandra Daskalaki, Andrei Daniel Mihalca, Jan Votýpka, Pavel Hulva, David Modrý
BACKGROUND: Jackals are medium-sized canids from the wolf-like clade, exhibiting a unique combination of ancestral morphotypes, broad trophic niches, and close phylogenetic relationships with the wolf and dog. Thus, they represent a potential host of several pathogens with diverse transmission routes. Recently, populations of the Eurasian golden jackal Canis aureus have expanded into the Western Palaearctic, including most of Europe. The aim of our study was to examine Eurasian golden jackals from Romania, Czech Republic and Austria for a wide spectrum of vector-borne protists and to evaluate the role of this species as a reservoir of disease for domestic dogs and/or humans...
April 14, 2017: Parasites & Vectors
https://www.readbyqxmd.com/read/28376903/the-prevalence-and-impact-of-babesia-canis-and-theileria-sp-in-free-ranging-grey-wolf-canis-lupus-populations-in-croatia
#14
Ana Beck, Doroteja Huber, Adam Polkinghorne, Andrea Gudan Kurilj, Valerija Benko, Vladimir Mrljak, Slaven Reljić, Josip Kusak, Irena Reil, Relja Beck
BACKGROUND: Babesia spp. and Theileria spp. are important emerging causes of disease in dogs. Alongside these domesticated hosts, there is increasing recognition that these piroplasms can also be found in a range of wild animals with isolated reports describing the presence of these pathogen in foxes (Vulpes vulpes) and captive grey wolves (Canis lupus). The prevalence and impact of these infections in free-ranging populations of canids are unknown. To gain a better insight into the epidemiology and pathogenesis of piroplasm infections in free-ranging grey wolves, pathological and molecular investigations into captive and free-ranging grey wolves in Croatia were performed...
April 4, 2017: Parasites & Vectors
https://www.readbyqxmd.com/read/28361802/molecular-characterization-of-babesia-and-theileria-species-in-ticks-collected-in-the-outskirt-of-monte-romano-lazio-region-central-italy
#15
Luciano Toma, Marco Di Luca, Fabiola Mancini, Francesco Severini, Carmela Mariano, Giancarlo Nicolai, Valentina Laghezza Masci, Alessandra Ciervo, Anna Maria Fausto, Simone Mario Cacciò
BACKGROUND: In 2012-2013, an investigation was carried out in the Viterbo province, Lazio region, on ticks and tick-borne Apicomplexan protozoa of the Babesia and Theileria genera. This followed the reporting of high density of ticks by soldiers operating in a military shooting range, and the signaling by owners and local veterinary authorities of several cases of babesiosis among cattle. METHODS: A total of 422 ticks were collected from 35 heads, whereas 96 ticks were collected by dragging...
January 2017: Annali Dell'Istituto Superiore di Sanità
https://www.readbyqxmd.com/read/28358861/pcr-diagnosis-of-tick-borne-pathogens-in-maharashtra-state-india-indicates-fitness-cost-associated-with-carrier-infections-is-greater-for-crossbreed-than-native-cattle-breeds
#16
Sunil W Kolte, Stephen D Larcombe, Suresh G Jadhao, Swapnil P Magar, Ganesh Warthi, Nitin V Kurkure, Elizabeth J Glass, Brian R Shiels
Tick-borne pathogens (TBP) are responsible for significant economic losses to cattle production, globally. This is particularly true in countries like India where TBP constrain rearing of high yielding Bos taurus, as they show susceptibility to acute tick borne disease (TBD), most notably tropical theileriosis caused by Theileria annulata. This has led to a programme of cross breeding Bos taurus (Holstein-Friesian or Jersey) with native Bos indicus (numerous) breeds to generate cattle that are more resistant to disease...
2017: PloS One
https://www.readbyqxmd.com/read/28355109/identification-of-12-piroplasms-infecting-ten-tick-species-in-china-using-reverse-line-blot-hybridization
#17
Mirza Omar Abdallah, Qingli Niu, Jifei Yang, Muhammad Adeel Hassan, Peifa Yu, Guiquan Guan, Ze Chen, Guangyuan Liu, Jianxun Luo, Hong Yin
Piroplasmosis, a disease of domestic and wild animals is caused by tick-borne protozoa in the genera of Theileria and Babesia. There was limited information available about the prevalence of piroplasmosis in ticks in China. To assess potential threat of piroplasmosis in China, we investigated the infections of ovine and bovine Babesia and Theileria species in ticks collected from cattle, yaks, sheep, horses and camels in several regions of China where tick-borne diseases have been reported. A total of 652 ticks were collected from the animals in 6 provinces of China...
March 13, 2017: Journal of Parasitology
https://www.readbyqxmd.com/read/28351721/a-rapid-simple-and-sensitive-loop-mediated-isothermal-amplification-method-to-detect-anaplasma-bovis-in-sheep-and-goats-samples
#18
Jinhong Wang, Yan Zhang, Yanyan Cui, Yaqun Yan, Xiaoxing Wang, Rongjun Wang, Fuchun Jian, Longxian Zhang, Changshen Ning
A loop-mediated isothermal amplification (LAMP) technique has been widely used in detecting the nucleic acid of various pathogenic bacteria. In this study, a set of four LAMP primers was designed to specifically test Anaplasma bovis. The LAMP assay was performed at 62°C for 60min in a water bath. The specificity was confirmed by amplifying A. bovis isolate, while no cross reaction was observed with other five pathogens (Anaplasma bovis, Anaplasma phagocytophilum, Theileria luwenshuni, Babesia motasi and Schistosoma japonicum)...
March 25, 2017: Parasitology International
https://www.readbyqxmd.com/read/28335825/canine-tick-borne-diseases-in-pet-dogs-from-romania
#19
Martin O Andersson, Conny Tolf, Paula Tamba, Mircea Stefanache, Jonas Waldenström, Gerhard Dobler, Lidia Chițimia-Dobler
BACKGROUND: Tick-borne diseases are of substantial concern worldwide for animals as well as humans. Dogs have been a human companion for millennia, and their significant impact on human life renders disease in dogs to be of great concern. Tick-borne diseases in dogs represent a substantial diagnostic challenge for veterinarians in that clinical signs are often diffuse and overlapping. In addition, co-infections with two or more pathogens enhance this problem further. Molecular methods are useful to disentangle co-infections and to accurately describe prevalence and geographical distribution of tick-borne diseases...
March 23, 2017: Parasites & Vectors
https://www.readbyqxmd.com/read/28316415/molecular-and-pathological-studies-on-natural-cases-of-bovine-theileriosis
#20
Anamika Gupta, Kuldip Gupta, Geeta Devi Leishangthem, Mandeep Singh Bal, N K Sood, Amarjit Singh
The present communication is a part of study conducted on 32 cases of bovine lymphadenopathies. Out of which, six cases of bovine theileriosis were diagnosed on the basis of peripheral blood smear examination and gross lesions in lymph nodes and visceral organs. Nested PCR using two primer sets N516/N517 and 14136/294 was conducted on samples collected from affected lymph nodes confirms Theileria annulata infection in five out of six cases. Sequencing analysis of amplified product showed 92, 94 and 93 % homology of isolate TH1_Bovine, TH2_Bovine and THEN_Bovine for T...
March 2017: Journal of Parasitic Diseases: Official Organ of the Indian Society for Parasitology
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