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https://www.readbyqxmd.com/read/27920212/inhibition-of-antigen-specific-and-non-specific-stimulation-of-bovine-t-and-b-cells-by-lymphostatin-from-attaching-and-effacing-escherichia-coli
#1
Robin L Cassady-Cain, Elizabeth A Blackburn, Charlotte R Bell, Elizaveta Elshina, Jayne C Hope, Mark P Stevens
Enterohaemorrhagic E. coli (EHEC) and enteropathogenic E. coli (EPEC) are enteric bacterial pathogens of worldwide importance. Most EPEC and non-O157 EHEC strains express lymphostatin (also known as LifA), a chromosomally-encoded 365 kDa protein. We previously demonstrated that lymphostatin is a putative glycosyltransferase that is important in intestinal colonisation of cattle by EHEC serogroup O5, O111 and O26 strains. However, the nature and consequences of the interaction between lymphostatin and immune cells from the bovine host are ill-defined...
December 5, 2016: Infection and Immunity
https://www.readbyqxmd.com/read/27913878/piroplasms-in-brown-hyaenas-parahyaena-brunnea-and-spotted-hyaenas-crocuta-crocuta-in-namibia-and-south-africa-are-closely-related-to-babesia-lengau
#2
Richard E J Burroughs, Barend L Penzhorn, Ingrid Wiesel, Nancy Barker, Ilse Vorster, Marinda C Oosthuizen
The objective of our study was identification and molecular characterization of piroplasms and rickettsias occurring in brown (Parahyaena brunnea) and spotted hyaenas (Crocuta crocuta) from various localities in Namibia and South Africa. Whole blood (n = 59) and skin (n = 3) specimens from brown (n = 15) and spotted hyaenas (n = 47) were screened for the presence of Babesia, Theileria, Ehrlichia and Anaplasma species using the reverse line blot (RLB) hybridization technique. PCR products of 52/62 (83...
December 2, 2016: Parasitology Research
https://www.readbyqxmd.com/read/27908771/molecular-detection-and-genetic-characterization-of-babesia-theileria-and-anaplasma-amongst-apparently-healthy-sheep-and-goats-in-the-central-region-of-turkey
#3
Mo Zhou, Shinuo Cao, Ferda Sevinc, Mutlu Sevinc, Onur Ceylan, Sepil Ekici, Charoonluk Jirapattharasate, Paul Franck Adjou Moumouni, Mingming Liu, Guanbo Wang, Aiko Iguchi, Patrick Vudriko, Hiroshi Suzuki, Xuenan Xuan
Babesia spp., Theileria spp. and Anaplasma spp. are significant tick-borne pathogens of livestock globally. In this study, we investigated the presence and distribution of Babesia ovis, Theileria ovis and Anaplasma ovis in 343 small ruminants (249 sheep and 94 goats) from 13 towns in the Central Anatolia region of Turkey using species-specific PCR assays. The PCR were conducted using the primers based on the B. ovis ssu rRNA (BoSSUrRNA), T. ovis ssu rRNA (ToSSUrRNA) and A. ovis major surface protein 4 (AoMSP4) genes, respectively...
November 16, 2016: Ticks and Tick-borne Diseases
https://www.readbyqxmd.com/read/27904685/phylogenetic-analysis-of-basic-helix-loop-helix-transcription-factors-in-the-genome-of-a-typical-human-disease-vector
#4
Meng-Yun Chen, Ying Dong, Rui-Xue Chang, Qian-Qian Ang, Ran Zhang, Yan-Yan Wu, Yi-Hui Xu, Wen-Sheng Lu, Xiao-Dong Zheng
Ixodes scapularis, the black-legged tick, is one of the most common human-disease vectors and transmits Borrelia species, such as B. burgdorferi, as well as Theileria microti, Anaplasma phagocytophilum, etc. As basic helix-loop-helix (bHLH) transcription factors have been recognized for many years as important regulators of various developmental processes, we performed phylogenetic analysis of the black-legged tick genome in order to identify the number and family of bHLH transcription factors. Because bHLH family members have been identified in many organisms, including silkworm and fruit fly, we were able to conduct this survey and identify 58 putative bHLH transcription factors...
2016: American Journal of Translational Research
https://www.readbyqxmd.com/read/27894847/pcr-screening-of-tick-borne-agents-in-sensitive-conservation-areas-southeast-portugal
#5
Maria Margarida Santos-Silva, Pedro Melo, Nuno Santos, Sandra Antunes, Luís Raposo Duarte, Joana Ferrolho, Natacha Milhano, Patrícia Tavares Santos, Ana Domingos, Ana Sofia Santos
The Southeast region of Portugal, particularly the Guadiana valley, is currently the reintroduction territory of Lynx pardinus (Iberian lynx), one of the most endangered felids in the world that is only found in the Iberian Peninsula. Over the last century, populations have declined, placing L. pardinus at extremely high risk of extinction in the wild and relying on reintroduction projects. Among the aspects taken into account in the establishment of new populations is the sanitary status of the selected habitats, especially concerning infectious diseases, including tick-borne pathogens (TBPs)...
November 25, 2016: Molecular and Cellular Probes
https://www.readbyqxmd.com/read/27890079/detection-of-theileria-parva-in-tissues-of-cattle-undergoing-severe-east-coast-fever-disease-show-significant-parasite-dna-accumulation-in-the-spleen
#6
Cassandra L Olds, Tasha Paul, Glen A Scoles
Infiltration and proliferation of Theileria parva infected lymphocytes in bovine host lymphoid organs is one of the hallmarks of T. parva infection. The relative abundance of parasites within infected host tissues, both lymphoid and non-lymphoid is however unknown. Using quantitative PCR, we have shown that significantly higher concentrations of T. parva DNA are detected in the spleens of cattle undergoing severe disease compared to other organs.
December 15, 2016: Veterinary Parasitology
https://www.readbyqxmd.com/read/27876977/a-study-on-prevalence-and-identification-of-ovine-theileria-and-babesia-infection-in-zabol-using-pcr-method
#7
Neda Sharifi, Maryam Ganjali, Reza Nabavi, Dariush Saadati
Infection with Babesia and Theileria Causes high mortality and economical losses in livestock and has a relatively high prevalence in Iran. In Zabol, animals are susceptible to this disease because of presence of vector ticks, weather conditions and smuggle animal across the border and they cause great damages to the economy and production. However, few studies have been done for differentiation of Theileria and Babesia in sheep in this area. The purpose of the present study was to determine the prevalence of Babesiosis and Theileriosis in sheep in Zabol...
December 2016: Journal of Parasitic Diseases: Official Organ of the Indian Society for Parasitology
https://www.readbyqxmd.com/read/27876976/variation-in-clinical-markers-in-cattle-naturally-infected-with-bovine-tropical-theileriosis
#8
Rahul Parashar, Vikrant Sudan, Amit Kumar Jaiswal, Daya Shanker
124 cattle naturally infected with Theileria annulata were inspected for clinical markers. Clinical manifestations of general weakness, reduced appetite, pyrexia reaching 40-42 °C and lachrymal discharge were seen in more than 75 % of the infected animals. Presence of ticks on body, general dullness and depression, recumbency/prostration, oedema of dependent body parts, diarrhea, hypersalivation, pale mucous membranes and yellow colored urine were revealed by more than 50 % of the infected animals. Clinical manifestations of dehydration, abdominal distension/ascites, jaundice and haemoglobinuria were revealed by more than 25 % of the infected animals less than 25 % of the infected animals showed clinical manifestations of exophthalmia, constipation, melena, buccal cavity erosions, congested mucous membranes, nasal discharge and tachypnoea...
December 2016: Journal of Parasitic Diseases: Official Organ of the Indian Society for Parasitology
https://www.readbyqxmd.com/read/27876944/seasonal-incidence-of-parasitic-diseases-in-bovines-of-south-western-gujarat-junagadh-india
#9
Binod Kumar, B R Maharana, Amit Prasad, Joice P Joseph, Bhavika Patel, J S Patel
The current study was conducted to investigate the incidence of parasitic diseases in bovines which were sick and brought at veterinary hospital for treatment. A total of 366 samples were investigated from cattle (n = 175) and buffaloes (n = 191) presented at Teaching Veterinary Clinical Complex (TVCC), Veterinary College, Junagadh, Gujarat during January to December 2014. Examination of Giemsa-stained peripheral blood smears exhibited that 58.6 % of cattle and 41.2 % of buffaloes were infected with haemoparasites comprising Babesia bigemina, Theileria annulata, and Anaplasma marginale @ of 54...
December 2016: Journal of Parasitic Diseases: Official Organ of the Indian Society for Parasitology
https://www.readbyqxmd.com/read/27876911/detection-of-theileria-annulata-carriers-in-holstein-friesian-bos-taurus-taurus-and-sistani-bos-taurus-indicus-cattle-breeds-by-polymerase-chain-reaction-in-sistan-region-iran
#10
Hamidreza Majidiani, Reza Nabavi, Maryam Ganjali, Dariush Saadati
Theileria annulata is common in tropical and subtropical regions especially in Iran and causes great economic losses in cattle industry. In Iran the epidemiological aspects of bovine theileriosis in different breeds of cattle is poorly understood. The aim of present study is comparison of the number of T. annulata carriers in the two major cattle breeds (Holstein-Friesian and Sistani) in Sistan of Iran by giemsa and polymerase chain reaction (PCR) methods. During winter 2013, 160 native cattle, from the two major breeds in Sistan, with the mean age of more than one year and without typical clinical symptoms of theileriosis were selected...
December 2016: Journal of Parasitic Diseases: Official Organ of the Indian Society for Parasitology
https://www.readbyqxmd.com/read/27863521/seroprevalence-of-bovine-theileriosis-in-northern-china
#11
Yaqiong Li, Zhijie Liu, Junlong Liu, Jifei Yang, Qian Li, Pengfei Guo, Guiquan Guan, Guangyuan Liu, Jianxun Luo, Hong Yin, Youquan Li
BACKGROUND: Bovine theileriosis is a common disease transmitted by ticks, and can cause loss of beef and dairy cattle worldwide. Here, an indirect enzyme-linked immunosorbent assay (iELISA) based on Theileria luwenshuni surface protein (TlSP) was developed and used to carry out a seroepidemiological survey of bovine theileriosis in northern China. METHODS: We used the BugBuster Ni-NTA His•Bind Purification Kit to purify recombinant TlSP (rTlSP), which was subsequently analyzed by Western Blotting to evaluate cross-reactivity with other pathogen-positive sera...
November 18, 2016: Parasites & Vectors
https://www.readbyqxmd.com/read/27845269/mitochondrial-genomes-of-two-babesia-taxa-from-sheep-in-china-as-a-foundation-for-population-genetic-and-epidemiological-investigations
#12
Tao Wang, Guiquan Guan, Pasi K Korhonen, Anson V Koehler, Neil D Young, Ross S Hall, Hong Yin, Robin B Gasser
Here, we sequenced, assembled and annotated the mitochondrial (mt) genomes of two operational taxonomic units of Babesia from sheep from China using a deep sequencing-coupled approach. Then, we defined and compared the gene order of these mt genomes (~5.8 to 6.2kb in size), assessed sequence differences in mt genes among Babesia taxa and evaluated genetic relationships among these taxa and related apicomplexans (Theileria) for which mt genomic data sets were available. We also identified mt genetic regions that might be useful as markers for future population genetic and molecular epidemiological studies of Babesia from small ruminants...
November 11, 2016: Infection, Genetics and Evolution
https://www.readbyqxmd.com/read/27835919/pka-and-apicomplexan-parasite-diseases
#13
M Haidar, G Ramdani, E J Kennedy, G Langsley
The cAMP-dependent protein kinase PKA is a well-characterized member of the serine-threonine protein AGC kinase family and is the effector kinase of cAMP signaling. As such, PKA is involved in the control of a wide variety of cellular processes including metabolism, cell growth, gene expression and apoptosis. cAMP-dependent PKA signaling pathways play important roles during infection and virulence of various pathogens. Since fluxes in cAMP are involved in multiple intracellular functions, a variety of different pathological infectious processes can be affected by PKA signaling pathways...
November 11, 2016: Hormone and Metabolic Research, Hormon- und Stoffwechselforschung, Hormones et Métabolisme
https://www.readbyqxmd.com/read/27832128/mitochondrial-genome-sequences-and-structures-aid-in-the-resolution-of-piroplasmida-phylogeny
#14
Megan E Schreeg, Henry S Marr, Jaime L Tarigo, Leah A Cohn, David M Bird, Elizabeth H Scholl, Michael G Levy, Brian M Wiegmann, Adam J Birkenheuer
The taxonomy of the order Piroplasmida, which includes a number of clinically and economically relevant organisms, is a hotly debated topic amongst parasitologists. Three genera (Babesia, Theileria, and Cytauxzoon) are recognized based on parasite life cycle characteristics, but molecular phylogenetic analyses of 18S sequences have suggested the presence of five or more distinct Piroplasmida lineages. Despite these important advancements, a few studies have been unable to define the taxonomic relationships of some organisms (e...
2016: PloS One
https://www.readbyqxmd.com/read/27832060/comparative-bioinformatics-analysis-of-transcription-factor-genes-indicates-conservation-of-key-regulatory-domains-among-babesia-bovis-babesia-microti-and-theileria-equi
#15
REVIEW
Heba F Alzan, Donald P Knowles, Carlos E Suarez
Apicomplexa tick-borne hemoparasites, including Babesia bovis, Babesia microti, and Theileria equi are responsible for bovine and human babesiosis and equine theileriosis, respectively. These parasites of vast medical, epidemiological, and economic impact have complex life cycles in their vertebrate and tick hosts. Large gaps in knowledge concerning the mechanisms used by these parasites for gene regulation remain. Regulatory genes coding for DNA binding proteins such as members of the Api-AP2, HMG, and Myb families are known to play crucial roles as transcription factors...
November 2016: PLoS Neglected Tropical Diseases
https://www.readbyqxmd.com/read/27825733/a-comparison-of-dna-extraction-protocols-from-blood-spotted-on-fta-cards-for-the-detection-of-tick-borne-pathogens-by-reverse-line-blot-hybridization
#16
Zerihun Hailemariam, Jabbar Sabir Ahmed, Peter-Henning Clausen, Ard Menzo Nijhof
An essential step in the molecular detection of tick-borne pathogens (TBPs) in blood is the extraction of DNA. When cooled storage of blood under field conditions prior to DNA extraction in a dedicated laboratory is not possible, the storage of blood on filter paper forms a promising alternative. We evaluated six DNA extraction methods from blood spotted on FTA Classic(®) cards (FTA cards), to determine the optimal protocol for the subsequent molecular detection of TBPs by PCR and the Reverse Line Blot hybridization assay (RLB)...
January 2017: Ticks and Tick-borne Diseases
https://www.readbyqxmd.com/read/27817011/genetic-characterization-of-theileria-orientalis-from-cattle-in-the-republic-of-korea
#17
Jinho Park, Yu-Jung Han, Du-Gyeong Han, Jeong-Byoung Chae, Joon-Seok Chae, Do-Hyeon Yu, Young-Sung Lee, Bae-Keun Park, Hyeon-Cheol Kim, Kyoung-Seong Choi
Theileria orientalis is the causative agent of benign theileriosis, which is distributed mainly in Asian countries, and causes serious economic losses in the livestock industry. The present study was performed to investigate the epidemiology of T. orientalis infections in cattle in the Republic of Korea (ROK) and to characterize the genetic diversity of T. orientalis based on the major piroplasm surface protein (MPSP) genes. In 2015, between July and August, blood samples were collected from 138 asymptomatic cattle in four different geographical regions (Hoengseong, Jeongeup, Namwon, and Jeju island) in the ROK...
November 6, 2016: Parasitology Research
https://www.readbyqxmd.com/read/27810201/oxidative-stress-and-dna-damage-in-horses-naturally-infected-with-theileria-equi
#18
M Radakovic, D Davitkov, S Borozan, S Stojanovic, J Stevanovic, V Krstic, Z Stanimirovic
The aim of this study was to determine the concentrations of oxidative stress parameters and DNA damage in horses infected by Theileria equi. Initial screening of 110 horses with duplex PCR enabled the selection of 30 infected horses with T. equi and 30 free of infection (control). Specimens from the 60 horses were further analysed by determining the following oxidative stress parameters: extent of haemolysis (EH), plasma free haemoglobin (PHb), catalase (CAT), Cu,Zn superoxide dismutase (SOD1), paraoxonase (PON1), nitrite (NO2(-)), total nitrate and nitrite (NOx), malondialdehyde (MDA) and free thiol groups (-SH)...
November 2016: Veterinary Journal
https://www.readbyqxmd.com/read/27809988/babesia-ovata-taxonomy-phylogeny-and-epidemiology
#19
REVIEW
Thillaiampalam Sivakumar, Ikuo Igarashi, Naoaki Yokoyama
Babesia ovata, which is transmitted by Haemaphysalis longicornis, is an intraerythrocytic protozoan parasite of cattle. Based on its morphology, B. ovata is classified as a large-type Babesia. The developmental stages of B. ovata have been described both in cattle and the tick vector. In infected adult female ticks, the parasite is transovarially transmitted to the tick eggs. The sexual reproduction of B. ovata has been demonstrated in the tick midgut. The diagnostic tools that are currently available for the specific detection of B...
October 15, 2016: Veterinary Parasitology
https://www.readbyqxmd.com/read/27809975/detection-of-theileria-orientalis-in-mosquito-blood-meals-in-the-united-kingdom
#20
M Fernández de Marco, V A Brugman, L M Hernández-Triana, L Thorne, L P Phipps, N I Nikolova, A R Fooks, N Johnson
Theileria spp. are tick-borne protozoan parasites that infect a wide range of wild and domestic animals. In this study, the utility of xenosurveillance of blood-fed specimens of Culiseta annulata for detecting the presence of piroplasms in livestock was investigated. Blood-fed mosquitoes were collected at Elmley National Nature Reserve, Kent, United Kingdom. All specimens were morphologically identified, and DNA barcoding was used to confirm the morphological identification. Both the vertebrate host species and Theileria genome was detected within the bloodmeal by real-time PCR...
October 15, 2016: Veterinary Parasitology
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