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https://www.readbyqxmd.com/read/28335825/canine-tick-borne-diseases-in-pet-dogs-from-romania
#1
Martin O Andersson, Conny Tolf, Paula Tamba, Mircea Stefanache, Jonas Waldenström, Gerhard Dobler, Lidia Chițimia-Dobler
BACKGROUND: Tick-borne diseases are of substantial concern worldwide for animals as well as humans. Dogs have been a human companion for millennia, and their significant impact on human life renders disease in dogs to be of great concern. Tick-borne diseases in dogs represent a substantial diagnostic challenge for veterinarians in that clinical signs are often diffuse and overlapping. In addition, co-infections with two or more pathogens enhance this problem further. Molecular methods are useful to disentangle co-infections and to accurately describe prevalence and geographical distribution of tick-borne diseases...
March 23, 2017: Parasites & Vectors
https://www.readbyqxmd.com/read/28316415/molecular-and-pathological-studies-on-natural-cases-of-bovine-theileriosis
#2
Anamika Gupta, Kuldip Gupta, Geeta Devi Leishangthem, Mandeep Singh Bal, N K Sood, Amarjit Singh
The present communication is a part of study conducted on 32 cases of bovine lymphadenopathies. Out of which, six cases of bovine theileriosis were diagnosed on the basis of peripheral blood smear examination and gross lesions in lymph nodes and visceral organs. Nested PCR using two primer sets N516/N517 and 14136/294 was conducted on samples collected from affected lymph nodes confirms Theileria annulata infection in five out of six cases. Sequencing analysis of amplified product showed 92, 94 and 93 % homology of isolate TH1_Bovine, TH2_Bovine and THEN_Bovine for T...
March 2017: Journal of Parasitic Diseases: Official Organ of the Indian Society for Parasitology
https://www.readbyqxmd.com/read/28316407/theileria-annulata-induced-brisket-oedema-in-a-bull-and-its-successful-treatment
#3
B Sudhakara Reddy, S Sivajothi
In the present case, jugular vein engorgement and oedema of the brisket region which mimic the signs of pseudopericarditis due to theileriosis in a bull was reported. An adult Ongole crossbred bull with history of weakness, anorexia and oedema at brisket region was referred to Teaching Veterinary Clinical Complex, College of Veterinary Science, Proddatur. Physical examination revealed the presence of ticks over the body, right and left prescapular lymphadenopathy and oedema at brisket region. Blood examination revealed the presence of piroplasms in erythrocytes, schizont in lymphocytes, anaemia and lymphopenia...
March 2017: Journal of Parasitic Diseases: Official Organ of the Indian Society for Parasitology
https://www.readbyqxmd.com/read/28288762/horses-infected-by-piroplasms-different-from-babesia-caballi-and-theileria-equi-species-identification-and-risk-factors-analysis-in-italy
#4
Stefania Zanet, Marina Bassano, Anna Trisciuoglio, Ivo Taricco, Ezio Ferroglio
Equine Piroplasmosis (EP) caused by Theileria equi and Babesia caballi is a disease affecting the health and the international movement of horses. In order to assess prevalence of Piroplasmid infection in the Northwestern part of Italy and to evaluate the associated risk factors, whole blood was collected from 135 horses from 7 different stables across the study area. PCR and sequencing were used to assess prevalence of infection and to identify detected Piroplasms to species level. A total of 23 horses (P=17...
March 15, 2017: Veterinary Parasitology
https://www.readbyqxmd.com/read/28286324/effects-of-dihydroorotate-dehydrogenase-dhodh-inhibitors-on-the-growth-of-theileria-equi-and-babesia-caballi-in%C3%A2-vitro
#5
Ketsarin Kamyingkird, Shinuo Cao, Bumduuren Tuvshintulga, Akram Salama, Ahmed Abdelmoniem Mousa, Artemis Efstratiou, Yoshifumi Nishikawa, Naoaki Yokoyama, Ikuo Igarashi, Xuenan Xuan
Theileria equi and Babesia caballi are the causative agents of equine piroplasmosis (EP), which affects equine production in various parts of the world. However, a safe and effective drug is not currently available for treatment of EP. Dihydroorotate dehydrogenase (DHODH) is the fourth enzyme in the de novo pyrimidine synthesis pathway and has been known as a novel drug target for several apicomplexan protozoan parasites. In this study, we evaluated four DHODH inhibitors; atovaquone (ATV), leflunomide (LFN), brequinar (Breq), and 7-hydroxy-5-[1,2,4] triazolo [1,5,a] pyrimidine (TAZ) on the growth of T...
March 9, 2017: Experimental Parasitology
https://www.readbyqxmd.com/read/28286144/characterization-of-theileria-equi-genotypes-in-horses-in-israel-the-palestinian-authority-and-jordan
#6
Dafna Ketter-Ratzon, Sharon Tirosh-Levy, Yaarit Nachum-Biala, Tal Saar, Lara Qura'n, Doni Zivotofsky, Ziad Abdeen, Gad Baneth, Amir Steinman
Equine theileriosis caused by Theileria equi is endemic in the Middle East, where it causes a severe disease as well as widespread subclinical infection. The aim of this study was to evaluate the diversity of T. equi genotypes in Israel and the neighboring Palestinian Authority and Jordan. Blood samples from 355 horses from Israel, the Palestinian Authority and Jordan were tested for the prevalence of T. equi DNA. Two hundred and fourteen (60%) were found positive for T. equi infection by PCR. Of those, the 18S rRNA (1458bp) and the EMA-1 (745bp) genes of T...
March 4, 2017: Ticks and Tick-borne Diseases
https://www.readbyqxmd.com/read/28285591/black-backed-jackals-canis-mesomelas-are-natural-hosts-of-babesia-rossi-the-virulent-causative-agent-of-canine-babesiosis-in-sub-saharan-africa
#7
Barend L Penzhorn, Ilse Vorster, Robert F Harrison-White, Marinda C Oosthuizen
BACKGROUND: Babesia rossi, which is transmitted by Haemaphysalis spp. and is highly virulent to domestic dogs, occurs only in sub-Saharan Africa. Since dogs are not native to the region, it has been postulated that the natural host of B. rossi is an indigenous African canid. Although various attempts at artificial infection indicated that black-backed jackals (Canis mesomelas) could become subclinically infected with B. rossi, data on occurrence of B. rossi in free-ranging jackals was lacking...
March 13, 2017: Parasites & Vectors
https://www.readbyqxmd.com/read/28281774/parasites-of-domestic-and-wild-animals-in-south-africa-l-ixodid-ticks-infesting-horses-and-donkeys
#8
Ivan G Horak, Heloise Heyne, Ali Halajian, Shalaine Booysen, Willem J Smit
The aim of the study was to determine the species spectrum of ixodid ticks that infest horses and donkeys in South Africa and to identify those species that act as vectors of disease to domestic livestock. Ticks were collected opportunistically from 391 horses countrywide by their owners or grooms, or by veterinary students and staff at the Faculty of Veterinary Science, University of Pretoria. Ticks were also collected from 76 donkeys in Limpopo Province, 2 in Gauteng Province and 1 in North West province...
February 28, 2017: Onderstepoort Journal of Veterinary Research
https://www.readbyqxmd.com/read/28249769/rapid-diagnosis-of-theileria-annulata-by-recombinase-polymerase-amplification-combined-with-a-lateral-flow-strip-lf-rpa-in-epidemic-regions
#9
Fangyuan Yin, Junlong Liu, Aihong Liu, Youquan Li, Jianxun Luo, Guiquan Guan, Hong Yin
Rapid and accurate diagnosis of Theileria annulata infection contributes to the formulation of strategies to eradicate this parasite. A simple and efficient diagnostic tool, recombinase polymerase amplification (RPA) combined with a lateral flow (LF) strip, was used in detection of Theileria and compared to other methods that require expensive instruments and skilled personnel. Herein, we established and optimized an LF-RPA method to detect the cytochrome b gene of T. annulata mitochondrial DNA from experimentally infected and field-collected blood samples...
February 21, 2017: Veterinary Parasitology
https://www.readbyqxmd.com/read/28231338/genes-encoding-two-theileria-parva-antigens-recognized-by-cd8-t-cells-exhibit-sequence-diversity-in-south-sudanese-cattle-populations-but-the-majority-of-alleles-are-similar-to-the-muguga-component-of-the-live-vaccine-cocktail
#10
Diaeldin A Salih, Roger Pelle, Joram M Mwacharo, Moses N Njahira, Wani L Marcellino, Henry Kiara, Agol K Malak, Abdel Rahim M El Hussein, Richard Bishop, Robert A Skilton
East Coast fever (ECF), caused by Theileria parva infection, is a frequently fatal disease of cattle in eastern, central and southern Africa, and an emerging disease in South Sudan. Immunization using the infection and treatment method (ITM) is increasingly being used for control in countries affected by ECF, but not yet in South Sudan. It has been reported that CD8+ T-cell lymphocytes specific for parasitized cells play a central role in the immunity induced by ITM and a number of T. parva antigens recognized by parasite-specific CD8+ T-cells have been identified...
2017: PloS One
https://www.readbyqxmd.com/read/28188085/species-of-ticks-and-carried-pathogens-in-owned-dogs-in-spain-results-of-a-one-year-national-survey
#11
Agustín Estrada-Peña, Xavier Roura, Angel Sainz, Guadalupe Miró, Laia Solano-Gallego
This study presents the results of a national survey in Spain on the distribution of ticks on owned dogs, their phenology and the associated pathogens over one year. In the study, 1628 adult ticks were collected on 660 dogs presented to 26 veterinary practices, of which 507 dogs (76.8%) carried at least one adult tick. The primary species of ticks were Rhipicephalus sanguineus s.l. (53%), Dermacentor reticulatus (9%), Ixodes ricinus (9%), and I. hexagonus (4%). Parasitism by two species of these ticks was rare...
February 3, 2017: Ticks and Tick-borne Diseases
https://www.readbyqxmd.com/read/28169066/standardization-and-validation-of-simple-pcr-duplex-pcr-and-rapd-in-comparison-to-blood-smear-examination-for-diagnosing-bovine-tropical-theileriosis
#12
Vikrant Sudan, Daya Shanker, Amit Jaiswal, Amit Singh, Vijay Pandey
Bovine Tropical Theileriosis (BTT) is an important vector-borne protozoan disease that imposing serious constraints on the health and productivity of domestic cattle. It is matter of common fact that following recovery from primary infection, cattle become persistent carriers and act as reservoirs of infection thereby, playing a critical role in disease epidemiology. The present study describes the comparative diagnostic efficiency of simplex PCR, duplex PCR and RAPD assays for detection of Theileria annulata in cattle...
February 3, 2017: Biologicals: Journal of the International Association of Biological Standardization
https://www.readbyqxmd.com/read/28155040/-what-makes-a-parasite-transforming-insights-into-cancer-from-the-agents-of-an-exotic-pathology-theileria-spp
#13
K M Cheeseman, J B Weitzman
Theileria are obligate eukaryotic intracellular parasites of cattle. The diseases they cause, Tropical theileriosis and East Coast Fever, cause huge economic loss in East African, Mediterranean and central and South-East Asian countries. These apicomplexan parasites are the only intracellular eukaryotic parasites known to transform their host cell and represent a unique model to study host-parasite interactions and mechanisms of cancer onset. Here, we review how Theileria parasites induce transformation of their leukocyte host cell and discuss similarities with tumorigenesis...
February 2, 2017: Bulletin de la Société de Pathologie Exotique
https://www.readbyqxmd.com/read/28153803/development-of-duplex-pcr-for-simultaneous-detection-of-theileria-spp-and-anaplasma-spp-in-sheep-and-goats
#14
Yanyan Cui, Yan Zhang, Fuchun Jian, Longxian Zhang, Rongjun Wang, Shuxuan Cao, Xiaoxing Wang, Yaqun Yan, Changshen Ning
Theileria spp. and Anaplasma spp., which are important tick-borne pathogens (TBPs), impact the health of humans and animals in tropical and subtropical areas. Theileria and Anaplasma co-infections are common in sheep and goats. Following alignment of the relevant DNA sequences, two primer sets were designed to specifically target the Theileria spp. 18S rRNA and Anaplasma spp. 16S rRNA gene sequences. Genomic DNA from the two genera was serially diluted tenfold for testing the sensitivities of detection of the primer sets...
January 31, 2017: Experimental Parasitology
https://www.readbyqxmd.com/read/28151085/natural-infection-of-the-south-american-tapir-tapirus-terrestris-by-theileria-equi
#15
Alexandre Welzel Da Silveira, Gustavo Gomes De Oliveira, Leandro Menezes Santos, Lucas Bezerra da Silva Azuaga, Claudia Regina Macedo Coutinho, Jessica Teles Echeverria, Tamires Ramborger Antunes, Carlos Alberto do Nascimento Ramos, Alda Izabel de Souza
Theileria equi is a tick-borne piroplasm considered endemic in equines in Brazil. The cohabitation of domestic and wild animals in areas of extensive cattle breeding favors the close contact between different species and the sharing of vectors and, consequently, pathogens. We report the natural infection of a young South American tapir ( Tapirus terrestris ) by Theileria equi in Mato Grosso do Sul, Brazil. Although it was not possible to associate the clinical and hematologic status of the animal with the infection by the protozoan parasite, our report represents an alert on the sharing of pathogens between domestic and wild animals...
February 2, 2017: Journal of Wildlife Diseases
https://www.readbyqxmd.com/read/28131376/molecular-evidence-of-tick-borne-hemoprotozoan-parasites-theileria-ovis-and-babesia-ovis-and-bacteria-in-ticks-and-blood-from-small-ruminants-in-northern-algeria
#16
Atef Aouadi, Hamza Leulmi, Mehdi Boucheikhchoukh, Ahmed Benakhla, Didier Raoult, Philippe Parola
Using qPCR, standard PCR and/or sequencing, we investigated the presence of tick-associated microorganisms in ticks and blood from sheep and goats from Souk Ahras, Algeria. Borrelia theileri, was detected in (7/120, 5.8%) blood from sheep and (13/120, 10.8%) goats. Anaplasma ovis was screened in (38/73, 52%) Rhipicephalus bursa and (5/22, 22.7%) R. turanicus and in (74/120, 61.7%), (65/120, 54.2%) blood of sheep and goats respectively. Coxiella burnetii tested positive in R. bursa (4/73, 5.5%) and (7/120, 5...
February 2017: Comparative Immunology, Microbiology and Infectious Diseases
https://www.readbyqxmd.com/read/28127369/molecular-identification-of-hemoprotozoan-parasites-in-camels-camelus-dromedarius-of-iran
#17
Alireza Sazmand, Barbara Eigner, Mohammad Mirzaei, Seyed Hossein Hekmatimoghaddam, Josef Harl, Georg Gerhard Duscher, Hans-Peter Fuehrer, Anja Joachim
BACKGROUND: Although camels represent a valuable source of food, wool and hide in many countries, in-depth information about their vector-borne pathogens is scarce compared to other animals. The aim of the current study was to characterize vector-borne protozoa in the blood of dromedaries from Iran by molecular tools. METHODS: From June to July 2014, 200 peripheral blood samples were collected from asymptomatic one-humped camels in two provinces of Kerman and Sistan- va-Baloochestan in central and southeastern Iran...
October 2016: Iranian Journal of Parasitology
https://www.readbyqxmd.com/read/28118556/investigation-into-individual-health-and-exposure-to-infectious-agents-of-platypuses-ornithorhynchus-anatinus-in-two-river-catchments-in-northwest-tasmania
#18
James W Macgregor, Carly S Holyoake, Sarah A Munks, Joanne H Connolly, Ian D Robertson, Patricia A Fleming, Kristin S Warren
Changes in the health of individuals within wildlife populations can be a cause or effect of population declines in wildlife species. Aspects of individual platypus ( Ornithorhynchus anatinus ) health have been reported. However, holistic studies investigating potential synergistic effects of both pathogens and environmental factors are needed to expand understanding of platypus individual health. We collected baseline data on the health of platypuses in two Tasmanian river catchments (including evidence of the potentially fatal fungal disease mucormycosis) and on individual, demographic, geographic, and temporal patterns associated with health data results...
January 24, 2017: Journal of Wildlife Diseases
https://www.readbyqxmd.com/read/28118097/a-loop-mediated-isothermal-amplification-lamp-assay-targeting-16s-rrna-gene-for-rapid-detection-of-anaplasma-phagocytophilum-infection-in-sheep-and-goats
#19
Changshen Ning, Jinhong Wang, Yan Zhang, Xiaoxing Wang, Yanyan Cui, Yaqun Yan, Rongjun Wang, Fuchun Jian, Longxian Zhang
Anaplasma phagocytophilum is a zoonotic pathogen and the causative agent of human granulocytic anaplasmosis (HGA) in humans and tick-borne fever in various kinds of animals. In the present study, a loop-mediated isothermal amplification (LAMP) assay for rapid detection of A. phagocytophilum was developed using primers specific to 16S rRNA gene of this organism. The LAMP assay was performed at 65 C for 60 min and terminated at 80 C for 10 min. The optimal reaction conditions, under which no cross-reaction was observed with other closely related tick borne parasites (Anaplasma bovis, Anaplasma ovis, Theileria luwenshuni, Babesia motasi and Schistosoma japonicum) was established...
January 24, 2017: Journal of Parasitology
https://www.readbyqxmd.com/read/28116531/dermacentor-reticulatus-a-putative-vector-of-babesia-cf-microti-syn-theileria-annae-piroplasm
#20
Adnan Hodžić, Johanna Zörer, Georg Gerhard Duscher
Babesia cf. microti (syn. Theileria annae, Babesia microti-like, Babesia vulpes) is a recently recognized tick-borne piroplasm that infects domestic and wild carnivores. Although Ixodes hexagonus is considered as the leading candidate responsible for the transmission, its capacity to act as a competent vector has not yet been confirmed. This study reports the occurrence of B.cf. microti in unfed Dermacentor reticulatus for the first time, suggesting that this tick species may be implicated in the life cycle of this canine parasite...
March 2017: Parasitology Research
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