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https://www.readbyqxmd.com/read/29439876/identification-of-theileria-fuliginosa-like-species-in-ixodes-australiensis-ticks-from-western-grey-kangaroos-macropus-fuliginosus-in-western-australia
#1
Siew-May Loh, Andrea Paparini, Una Ryan, Peter Irwin, Charlotte Oskam
Piroplasms, including the genera Babesia and Theileria, are intra-erythrocytic protozoa that are generally transmitted by ticks and are the aetiological agents for piroplasmosis in animals, as well as humans, worldwide. In Australia, numerous studies have been conducted on piroplasms in domestic animals; however, less is known about these protozoa in ticks from native wildlife. The present study characterised piroplasms in Ixodes australiensis (n = 119) and Amblyomma triguttatum (n = 35) ticks collected from kangaroos in Western Australia (WA)...
February 8, 2018: Ticks and Tick-borne Diseases
https://www.readbyqxmd.com/read/29429808/immune-parameters-to-p67c-antigen-adjuvanted-with-isa206vg-correlate-with-protection-against-east-coast-fever
#2
Anna Lacasta, Stephen Mwalimu, Elisabeth Kibwana, Rosemary Saya, Elias Awino, Thomas Njoroge, Jane Poole, Nicholas Ndiwa, Roger Pelle, Vishvanath Nene, Lucilla Steinaa
East Coast fever (ECF) is a lymphoproliferative disease caused by the tick-transmitted protozoan parasite Theileria parva. ECF is one of the most serious cattle tick-borne diseases in Sub-Saharan Africa. We have previously demonstrated that three doses of the C-terminal part of the sporozoite protein p67 (p67C) adjuvanted with ISA206VG confers partial protection against ECF at a herd level. We have tested the efficacy of two doses of this experimental vaccine, as reducing the vaccination regimen would facilitate its deployment in the field...
February 8, 2018: Vaccine
https://www.readbyqxmd.com/read/29426590/detection-and-molecular-characterization-of-tick-borne-pathogens-infecting-sheep-and-goats-in-blue-nile-and-west-kordofan-states-in-sudan
#3
Seung-Hun Lee, Ehab Mossaad, Abdalla Mohamed Ibrahim, Ahmed Ali Ismail, Paul Franck Adjou Moumouni, Mingming Liu, Aaron Edmond Ringo, Yang Gao, Huanping Guo, Jixu Li, Artemis Efstratiou, Peter Musinguzi, Tamador E E Angara, Keisuke Suganuma, Noboru Inoue, Xuenan Xuan
Tick-borne pathogens (TBPs) are common in livestock of sub-Saharan Africa. However, information regarding TBPs in sheep and goats in Sudan is limited. In this study, 178 blood samples of sheep and goats in Blue Nile and West Kordofan states were investigated for TBPs using PCR. Overall, 110 (61.8%) samples were found to be infected with at least one of the following pathogens: Anaplasma ovis, Theileria ovis, and Ehrlichia ruminantium. Babesia ovis and T. lestoquardi were not identified. A. ovis was the most prevalent pathogen (n = 107, 60...
February 1, 2018: Ticks and Tick-borne Diseases
https://www.readbyqxmd.com/read/29426483/in-vitro-growth-inhibition-of-theileria-equi-by-bumped-kinase-inhibitors
#4
Fernanda Gimenez, Siddra A Hines, Ryan Evanoff, Kayode K Ojo, Wesley C Van Voorhis, Dustin J Maly, Rama S R Vidadala, Robert H Mealey
INTRODUCTION: Theileria equi, an etiologic agent of equine piroplasmosis, is a tick-transmitted hemoprotozoan of the phylum Apicomplexa. Recent outbreaks of piroplasmosis in the United States have renewed interest in safe and effective treatment options. Although imidocarb dipropionate (IMD) is the drug of choice for clearance of T. equi, adverse reactions and recently documented resistance support the need for alternative therapeutic strategies. The recently described bumped kinase inhibitors (BKIs) are a new class of compounds that could potentially be used as safe and effective alternatives to IMD...
February 15, 2018: Veterinary Parasitology
https://www.readbyqxmd.com/read/29409517/isolation-and-purification-of-glycosylphosphatidylinositols-gpis-in-the-schizont-stage-of-theileria-annulata-and-determination-of-antibody-response-to-gpi-anchors-in-vaccinated-and-infected-animals
#5
Toktam Abbasnia, Ahmad Asoodeh, Gholamreza Habibi, Alireza Haghparast
BACKGROUND: Tropical theileriosis is widely distributed from North Africa to East Asia. It is a tick-borne disease caused by Theileria annulata, an obligate two-host intracellular protozoan parasite of cattle. Theileria annulata use leukocytes and red blood cells for completion of the life-cycle in mammalian hosts. The stage of Theileria annulata in monocytes and B lymphocytes of cattle is an important step in pathogenicity and diagnosis of the disease. Glycosylphosphatidylinositols (GPIs) are a distinct class of glycolipid structures found in eukaryotic cells and are implicated in several biological functions...
February 6, 2018: Parasites & Vectors
https://www.readbyqxmd.com/read/29408266/ancient-diversity-and-geographical-sub-structuring-in-african-buffalo-theileria-parva-populations-revealed-through-metagenetic-analysis-of-antigen-encoding-loci
#6
Johanneke D Hemmink, Tatjana Sitt, Roger Pelle, Lin-Mari de Klerk-Lorist, Brian Shiels, Philip G Toye, W Ivan Morrison, William Weir
An infection and treatment protocol involving infection with a mixture of three parasite isolates and simultaneous treatment with oxytetracycline is currently used to vaccinate cattle against Theileria parva. While vaccination results in high levels of protection in some regions, little or no protection is observed in areas where animals are challenged predominantly by parasites of buffalo origin. A previous study involving sequencing of two antigen-encoding genes from a series of parasite isolates indicated that this is associated with greater antigenic diversity in buffalo-derived T...
February 2, 2018: International Journal for Parasitology
https://www.readbyqxmd.com/read/29391689/prevalence-of-theileria-annulata-in-dairy-cattle-in-nyala-south-darfur-state-sudan
#7
Ismail A Abaker, Diaeldin A Salih, Lima M El Haj, Rawia E Ahmed, Manal M Osman, Awadia M Ali
Aim: This study was conducted in dairy cattle in Nyala, South Darfur State, during the period from June to September 2015, to study the prevalence of bovine tropical theileriosis. Materials and Methods: Apparently, healthy cattle of different age groups, different breeds, and from both sexes were randomly selected from seven locations. Three age groups of cattle were selected, group one <1 year old, group two 1-3 years old, and group three older than 3 years...
December 2017: Veterinary World
https://www.readbyqxmd.com/read/29391688/prevalence-of-carrier-state-theileriosis-in-lactating-cows
#8
Niranjana Sahoo, Bikash Kumar Behera, Hemant Kumar Khuntia, Manojita Dash
Aim: The objective of this study was to examine the carrier status of theileriosis among apparently healthy cross-bred jersey cattle population of Odisha using conventional blood smear examination and polymerase chain reaction (PCR). Materials and Methods: A total of 34 apparently healthy cross-bred Jersey lactating cows were considered in this study. Blood samples were subjected to microscopic examination after staining with Giemsa stain and PCR based molecular diagnosis using two sets of primer, i...
December 2017: Veterinary World
https://www.readbyqxmd.com/read/29373078/molecular-detection-of-theileria-annulata-in-cattle-from-different-regions-of-punjab-pakistan-using-recombinase-polymerase-amplification-and-pcr
#9
Muhammad Adeel Hassan, Junlong Liu, Muhammad Sohail Sajid, Altaf Mahmood, Shuai Yang Zhao, Qamar Abbas, Guiquan Guan, Hong Yin, Jianxun Luo
Piroplasmosis is one of the most important diseases of livestock, constraining optimal production and leading to economic loss. This study was carried out to detect Theileria annulata using 2 different molecular techniques: recombinase polymerase amplification (RPA) and conventional PCR. Blood samples were collected from 274 ticks infesting asymptomatic cattle from several counties of Chakwal, Faisalabad, and Jhang districts of Punjab province of Pakistan using FTA cards. Following extraction of genomic DNA, each sample was subjected to RPA optimized to amplify a 281 bp fragment of the Enolase gene for T...
January 26, 2018: Journal of Parasitology
https://www.readbyqxmd.com/read/29343295/cytotoxic-t-lymphocytes-from-cattle-sharing-the-same-mhc-class-i-haplotype-and-immunized-with-live-theileria-parva-sporozoites-differ-in-antigenic-specificity
#10
Lucilla Steinaa, Nicholas Svitek, Elias Awino, Rosemary Saya, Philip Toye
OBJECTIVES: The objective of this study was to assess whether cytotoxic T cells (CTL) generated by the live vaccine, known as "ITM Muguga cocktail", which is used for the cattle disease East Cost fever (ECF) in Sub-Saharan Africa, showed a broad reactivity against many different strains of the causative parasite Theileria parva. We also assessed whether immune responses were similar in cattle expressing the same MHC class I haplotypes. RESULTS: The antigenic specificity of CTL from MHC class I-matched cattle vaccinated with the Muguga cocktail were different...
January 17, 2018: BMC Research Notes
https://www.readbyqxmd.com/read/29337258/adding-injury-to-infection-the-relationship-between-injury-status-and-genetic-diversity-of-theileria-infecting-plains-zebra-equus-quagga
#11
Edward M King'ori, Vincent Obanda, Ephantus M Ndambiri, Steven M Runo, Patrick I Chiyo
Asymptomatic tick-borne infections are a common feature in wild herbivores. In human-dominated habitats, snare injuries to wild herbivores are common and are likely to co-occur with enzootic infections. The influence of injury on pattern, course and outcome of enzootic infection in wild herbivores is unknown. We identified Theileria species infecting zebra and assessed the relationship between host injury-status and parasitaemia, parasite diversity and selection regimes. We also determined host leucocyte differential as this can reveal mechanisms by which injuries influence infections...
January 11, 2018: Infection, Genetics and Evolution
https://www.readbyqxmd.com/read/29331578/molecular-detection-and-characterisation-of-babesia-and-theileria-in-australian-hard-ticks
#12
Brooke Storey-Lewis, Ann Mitrovic, Brent McParland
Babesia and Theileria are intraerythrocytic protozoans of the phylum Apicomplexa. These species are capable of infecting wild and domestic animals and have historically caused great economic loss in the agricultural industry. In recent years human babesiosis has been deemed an emerging zoonosis in North America, Europe and Asia. The first locally acquired case of babesiosis in Australia, caused by Babesia microti, was reported in March 2012. A number of native Babesia and Theileria species have been identified in Australian marsupials, however their associated tick vectors and threat to human health is unknown...
December 24, 2017: Ticks and Tick-borne Diseases
https://www.readbyqxmd.com/read/29322051/genetic-variation-of-the-%C3%AE-tubulin-gene-of-babesia-caballi-strains
#13
María Guadalupe Montes-Cortés, José Luis Fernández-García, Miguel Ángel Habela Martínez-Estéllez
Background: Equine piroplasmosis is caused by two haemoprotozoan parasites: Babesia caballi and Theileria equi. Negative economic impact on international trade has been associated to endemic sites. This is the reason why carrier detection requires reliable diagnostic methods. Various diagnostic modalities can be used alone or in combination including PCR. However, genetic variation of commonly used genes is still of debate. The aim of this research was to sequence the β-tubulin gene of a B...
September 2017: Journal of Arthropod-borne Diseases
https://www.readbyqxmd.com/read/29317885/seroprevalence-and-risk-factors-for-theileria-equi-infection-in-equines-from-khyber-pakhtunkhwa-province-pakistan
#14
Muhammad Jamal Khan Afridi, Abdul Hafeez Mian, Muhammad Saqib, Ghazanfar Abbas, Javid Ali, Muhammad Khalid Mansoor, Awais Ur Rahman Sial, Imaad Rasheed, Muhammad Hammad Hussain
Background: Theileria equi is a tick borne protozoan parasite which causes piroplasmosis among equines worldwide. The present study was aimed to determine seroprevalence of T. equi in donkeys, horses, and mules from two equine populated districts (Peshawar and Charsadda) of Khyber Pakhtunkhwa (KPK), Pakistan. Methods: A total of 393 equine (195 horses, 194 donkeys and 4 mules) serum samples were collected from five and four randomly selected localities in Charsadda (n = 193) and Peshawar (n = 200), respectively...
October 2017: Iranian Journal of Parasitology
https://www.readbyqxmd.com/read/29297802/prevalence-and-molecular-identification-of-piroplasmids-in-iranian-dromedaries-camelus-dromedarius
#15
Somayeh Bahrami, Mohammad Reza Tabandeh, Ali Reza Ganjali Tafreshi
Camels ( Camelus dromedarius) are important, multipurpose local animals in Iran. Despite their importance, camelid parasitic diseases have not received adequate attention in the veterinary literature. The present study investigated the prevalence of, and molecularly identified, camel piroplasms in Iran. Blood samples from 248 camels from five different regions of Iran were screened for the presence of piroplasmid infection using an 18SrRNA polymerase chain reaction (PCR) sequencing method. Of the 248 samples, 16 were positive for piroplasms via PCR (6...
December 2017: Journal of Zoo and Wildlife Medicine: Official Publication of the American Association of Zoo Veterinarians
https://www.readbyqxmd.com/read/29291385/serological-detection-and-molecular-characterization-of-piroplasmids-in-equids-in-brazil
#16
Maria Isabel Botelho Vieira, Márcio Machado Costa, Mateus Tonial de Oliveira, Luiz Ricardo Gonçalves, Marcos Rogério André, Rosangela Zacarias Machado
Equine piroplasmosis is a disease caused by the hemoparasites Babesia caballi and Theileria equi and is considered to be the most important parasitic infection affecting Equidae. The objective of the present study was to carry out an epidemiological molecular and serological survey for the presence of these two protozoal organisms in equids from the northwestern region of the State of Rio Grande do Sul (RS), south Brazil. For this purpose, blood samples were collected from 90 equids in the city of Passo Fundo, RS, Brazil...
March 2018: Acta Tropica
https://www.readbyqxmd.com/read/29258832/characterization-of-the-theileria-parva-sporozoite-proteome
#17
James Nyagwange, Edwin Tijhaar, Nicola Ternette, Fredrick Mobegi, Kyle Tretina, Joana C Silva, Roger Pelle, Vishvanath Nene
East Coast fever is a lymphoproliferative disease caused by the tick-borne protozoan parasite Theileria parva. The sporozoite stage of this parasite, harboured and released from the salivary glands of the tick Rhipicephalus appendiculatus during feeding, invades and establishes infection in bovine lymphocytes. Blocking this initial stage of invasion presents a promising vaccine strategy for control of East Coast fever and can in part be achieved by targeting the major sporozoite surface protein p67. To support research on the biology of T...
December 16, 2017: International Journal for Parasitology
https://www.readbyqxmd.com/read/29237791/multiplexed-tandem-pcr-mt-pcr-assay-using-the-major-piroplasm-surface-protein-gene-for-the-diagnosis-of-theileria-orientalis-infection-in-cattle
#18
Hagos Gebrekidan, Robin B Gasser, Mark A Stevenson, Abdul Jabbar
Oriental theileriosis is an economically important tick-borne disease of bovines, particularly in the Asia-Pacific region, and is caused by one or more genotypes of the Theileria orientalis complex (1, 2).….
December 13, 2017: Journal of Clinical Microbiology
https://www.readbyqxmd.com/read/29223587/molecular-characterization-of-theileria-equi-in-horses-from-the-state-of-rio-de-janeiro-brazil
#19
Maristela Peckle, Marcus Sandes Pires, Claudia Bezerra da Silva, Renata Lins da Costa, Gabriela Lopes Vivas Vitari, Marcus Vinicius Xavier Senra, Roberto Júnio Pedroso Dias, Huarrisson Azevedo Santos, Carlos Luiz Massard
Theileria equi is one of the etiologic agents of the equine piroplasmosis. This infectious disease is transmitted by ticks and is a worldwide problem in the international horse movement. The 18S rRNA gene of T. equi is often used for genotyping and phylogenetic purpose. This study aimed to analyze the degree of the heterogeneity of the 18S rRNA gene of T. equi in horses from the state of Rio de Janeiro, Brazil. The complete T. equi 18S rRNA sequences were obtained from twenty naturally infected horses. The PCR amplicons were cloned and sequenced...
November 28, 2017: Ticks and Tick-borne Diseases
https://www.readbyqxmd.com/read/29197596/historical-review-and-insights-on-the-livestock-tick-borne-disease-research-of-a-developing-country-the-philippine-scenario
#20
REVIEW
Adrian P Ybañez, Claro N Mingala, Rochelle Haidee D Ybañez
Tick-borne diseases (TBDs) remain to be a global animal health threat. Developing countries like the Philippines is not exempt to this. Despite the potential impact TBDs can give to these countries, local government initiatives and researches remain to be limited. In the Philippines, most epidemiological studies were confined only to specific areas, and predominantly in the Northern Area. Due to its unique geography and limited studies, the current nationwide status of most TBDs could not be clearly established...
November 29, 2017: Parasitology International
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