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https://www.readbyqxmd.com/read/29451362/simultaneous-multiple-ms-binding-assays-for-the-dopamine-norepinephrine-and-serotonin-transporters
#1
Patrick Neiens, Angela De Simone, Georg Höfner, Klaus T Wanner
In this work, we present label-free, mass-spectrometry-based binding assays (MS Binding Assays), targeting the human dopamine, norepinephrine, and serotonin transporters (hDAT, hNET, and hSERT) in simultaneous binding experiments. Using a validated LC-ESI-MS/MS method for quantification of the selective dopamine transporter inhibitor (R,R)-4-(2-benzhydryloxyethyl)-1-(4-fluorobenzyl)piperidin-3-ol ((R,R)-D-84), the selective norepinephrine transporter inhibitor (S,S)-reboxetine, and the selective serotonin reuptake inhibitor (S)-citalopram, binding affinities at the three monoamine transporters could be characterized simultaneously in a single binding experiment...
February 16, 2018: ChemMedChem
https://www.readbyqxmd.com/read/29451186/-lactobacillus-acidophilus-and-bifidobacterium-longum-supernatants-upregulate-the-serotonin-transporter-expression-in-intestinal-epithelial-cells
#2
Ya-Nan Cao, Li-Juan Feng, Bang-Mao Wang, Kui Jiang, Shu Li, Xin Xu, Wei-Qiang Wang, Jing-Wen Zhao, Yu-Ming Wang
Background/Aims: Probiotics play a role in relieving irritable bowel syndrome (IBS); however, the underlying mechanism is yet unclear. The aim of the study was to investigate the effects of the supernatants of Lactobacillus acidophilus and Bifidobacterium longum on the expression of serotonin transporter (SERT) messenger ribonucleic acid (mRNA) and protein. Materials and Methods: HT-29 and Caco-2 cells were treated with different concentrations of L. acidophilus and B...
January 2018: Saudi Journal of Gastroenterology: Official Journal of the Saudi Gastroenterology Association
https://www.readbyqxmd.com/read/29447434/alterations-in-melatonin-and-serotonin-signalling-in-the-colonic-mucosa-of-mice-with-dextran-sodium-sulfate-induced-colitis
#3
Sarah J MacEachern, Catherine M Keenan, Evangelia Papakonstantinou, Keith A Sharkey, Bhavik Anil Patel
BACKGROUND AND PURPOSE: Inflammatory bowel disease (IBD) is characterized by pain, bleeding, cramping and altered gastrointestinal (GI) function. Changes in mucosal serotonin (5-HT) signalling occur in animal models of colitis and in humans suffering from IBD. Melatonin is co-released with 5-HT from the mucosa and has a wide variety of actions in the GI tract. Here we examined how melatonin signalling is affected by colitis and determined how this relates to 5-HT signalling. EXPERIMENTAL APPROACH: Using electroanalytical approaches, we investigated how 5-HT release, reuptake and availability as well as melatonin availability are altered in dextran sodium sulfate (DSS)-induced colitis in mice...
February 15, 2018: British Journal of Pharmacology
https://www.readbyqxmd.com/read/29441949/effect-of-serotonin-transporter-genotype-on-self-reported-efficacy-and-activity-changes-of-brain-prefrontal-area-in-response-to-placebo
#4
M Isawa, R Tashiro, C Naruse, Y Yamaguchi, H Itoh, T Nishimura, M Tomi, H Shimada, H Saito, M Mochizuki, E Nakashima
Patients benefit from drug therapy not only through pharmacological mechanisms, but also through non-pharmacological action (placebo effect), which may be mediated in part by the prefrontal area of the brain. We consider that the difference between responders and non-responders to placebo might be related to polymorphisms in the serotonin transporter-linked polymorphic region (5-HTTLPR). To study this idea, we performed a randomized double-blind clinical trial using caffeine and lactose (placebo). Activity in the prefrontal area of the brain was measured in terms of blood flow by means of near-infrared spectroscopy (NIRS) as an objective indicator...
January 2, 2018: Die Pharmazie
https://www.readbyqxmd.com/read/29433608/brain-stem-serotonin-transporter-availability-in-maternal-uniparental-disomy-and-deletion-prader-willi-syndrome
#5
Rajeev Krishnadas, Sally-Ann Cooper, Alice Nicol, Sally Pimlott, Sarita Soni, Anthony J Holland, Laura McArthur, Jonathan Cavanagh
Prader-Willi syndrome (PWS) is a rare condition because of the deletion of paternal chromosomal material (del PWS), or a maternal uniparental disomy (mUPD PWS), at 15q11-13. Affective psychosis is more prevalent in mUPD PWS. We investigated the relationship between the two PWS genetic variants and brain-stem serotonin transporter (5-HTT) availability in adult humans. Mean brain-stem 5-HTT availability determined by [123I]-beta-CIT single photon emission tomography was lower in eight adults with mUPD PWS compared with nine adults with del PWS (mean difference -0...
January 2018: British Journal of Psychiatry: the Journal of Mental Science
https://www.readbyqxmd.com/read/29427306/impaired-fear-extinction-in-serotonin-transporter-knockout-rats-is-associated-with-increased-5-hydroxymethylcytosine-in-the-amygdala
#6
Ling Shan, Hang-Yuan Guo, Corina N A M van den Heuvel, Joop van Heerikhuize, Judith R Homberg
AIMS: One potential risk factor for posttraumatic stress disorder (PTSD) involves the low activity (short; s) allelic variant of the serotonin transporter-linked polymorphic region (5-HTTLPR), possibly due to reduced prefrontal control over the amygdala. Evidence shows that DNA methylation/demethylation is crucial for fear extinction in these brain areas and is associated with neuronal activation marker c-Fos expression. We hypothesized that impaired fear extinction in serotonin transporter knockout (5-HTT-/- ) rats is related to changes in DNA (de) methylation and c-Fos expression in the prefrontal cortex (PFC) and/or amygdala...
February 9, 2018: CNS Neuroscience & Therapeutics
https://www.readbyqxmd.com/read/29427069/new-scaffold-for-lead-compounds-to-treat-methamphetamine-use-disorders
#7
Na-Ra Lee, Guangrong Zheng, Peter A Crooks, Michael T Bardo, Linda P Dwoskin
Despite increased methamphetamine use worldwide, pharmacotherapies are not available to treat methamphetamine use disorder. The vesicular monoamine transporter-2 (VMAT2) is an important pharmacological target for discovery of treatments for methamphetamine use disorder. VMAT2 inhibition by the natural product, lobeline, reduced methamphetamine-evoked dopamine release, methamphetamine-induced hyperlocomotion, and methamphetamine self-administration in rats. Compared to lobeline, lobelane exhibited improved affinity and selectivity for VMAT2 over nicotinic acetylcholine receptors...
February 9, 2018: AAPS Journal
https://www.readbyqxmd.com/read/29427067/individual-biological-sensitivity-to-environmental-influences-testing-the-differential-susceptibility-properties-of-the-5httlpr-polymorphism-in-relation-to-depressive-symptoms-and-delinquency-in-two-adolescent-general-samples
#8
Cecilia Åslund, Kent W Nilsson
The gene-environment interaction research field in psychiatry has traditionally been dominated by the diathesis-stress framework, where certain genotypes are assumed to confer increased risk for adverse outcomes in a stressful environment. In later years, theories of differential susceptibility, or biological sensitivity, suggest that candidate genes that interact with environmental events do not exclusively confer a risk for behavioural or psychiatric disorders but rather seem to alter the sensitivity to both positive and negative environmental influences...
February 9, 2018: Journal of Neural Transmission
https://www.readbyqxmd.com/read/29423959/low-resilience-to-stress-is-associated-with-candidate-gene-expression-alterations-in-the-dopaminergic-signalling-pathway
#9
Esfandiar Azadmarzabadi, Arvin Haghighatfard, Alireza Mohammadi
BACKGROUND: In stressful situations, a person's ability to appropriately complete tasks with minimal anxiety is known as stress resilience. Genetic variants in neuropeptide Y, Corticotropin releasing hormone receptor 1 (CRHR1), and serotonin transporter have been previously reported to be associated with low resilience, but the relationship between low resilience and the dopaminergic signalling pathway is not well understood. Here, we aimed to describe the association between comprehensive psychological characteristics and messenger RNA levels of dopamine receptor D1 (DRD1), dopamine receptor D2 (DRD2), dopamine receptor D3 (DRD3), dopamine receptor D4 (DRD4), dopamine receptor D5 (DRD5), COMT, Dopamine Beta-Hydroxylase (DBH), Tyrosine hydroxylase (TH), monoamine oxidase A (MAOM), dopa decarboxylase (DDC), dopamine transporter (DAT), serotonin transporter (5-HTT), brain-derived neurotrophic factor (BDNF) and glial cell line-derived neurotrophic factor (GDNF) genes based on peripheral blood samples from 400 subjects who react differently to major life event stressors...
February 8, 2018: Psychogeriatrics: the Official Journal of the Japanese Psychogeriatric Society
https://www.readbyqxmd.com/read/29423392/neurochemical-alterations-in-sudden-unexplained-perinatal-deaths-a-review
#10
REVIEW
Nazeer Muhammad, Muhammad Sharif, Javeria Amin, Riffat Mehboob, Syed Amir Gilani, Nargis Bibi, Hasnain Javed, Naseer Ahmed
Sudden unexpected perinatal collapse is a major trauma for the parents of victims. Sudden infant death syndrome (SIDS) is unexpected and mysterious death of an apparently healthy neonate from birth till 1 year of age without any known causes, even after thorough postmortem investigations. However, the incidence of sudden intrauterine unexplained death syndrome (SIUDS) is seven times higher as compared with SIDS. This observation is approximated 40-80%. Stillbirth is defined as death of a fetus after 20th week of gestation or just before delivery at full term without a known reason...
2018: Frontiers in Pediatrics
https://www.readbyqxmd.com/read/29416077/prefrontal-cortex-modulates-the-correlations-between-brain-derived-neurotrophic-factor-level-serotonin-and-the-autonomic-nervous-system
#11
Wei Hung Chang, I Hui Lee, Mei Hung Chi, Shih-Hsien Lin, Kao Chin Chen, Po See Chen, Nan Tsing Chiu, Wei Jen Yao, Yen Kuang Yang
Top-down regulation in the human brain and anatomical connections between the prefrontal cortex (PFC) and specific catecholamine-related regions have been well-studied. However, the way in which the PFC modulates downstream neuro-networks in terms of serotonin and the autonomic nervous system (ANS) by variation in the level of brain-derived neurotrophic factor (BDNF) is still unclear. We recruited sixty-seven healthy subjects. Serotonin transporter (SERT) availability was examined by SPECT with [ 123 I]ADAM analysis; heart rate variability (HRV) testing was performed, and the BDNF level was measured...
February 7, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29414147/clobenpropit-a-histamine-h3-receptor-antagonist-inverse-agonist-inhibits-3h-dopamine-uptake-by-human-neuroblastoma-sh-sy5y-cells-and-rat-brain-synaptosomes
#12
Elvia Mena-Avila, Ricardo Márquez-Gómez, Guillermo Aquino-Miranda, Gustavo Nieto-Alamilla, José-Antonio Arias-Montaño
BACKGROUND: Clobenpropit, a potent antagonist/inverse agonist at the histamine H3 receptor (H3R), reduced the cytotoxic action of 6-hydroxydopamine (6-OHDA) in neuroblastoma SH-SY5Y cells transfected with the human H3R. We therefore set out to study whether this effect involved a receptor-independent action on dopamine transport. METHODS: The uptake of [3H]-dopamine was assayed in SH-SY5Y cells and rat striatal or cerebro-cortical isolated nerve terminals (synaptosomes)...
August 30, 2017: Pharmacological Reports: PR
https://www.readbyqxmd.com/read/29414128/the-effects-of-5-httlpr-and-bdnf-val66met-polymorphisms-on-neurostructural-changes-in-major-depressive-disorder
#13
Kyu-Man Han, Sunyoung Choi, Aram Kim, June Kang, Eunsoo Won, Woo-Suk Tae, Yong-Ku Kim, Min-Soo Lee, Byung-Joo Ham
The serotonin-transporter-linked polymorphic region (5-HTTLPR) and brain-derived neurotrophic factor (BDNF) Val66Met polymorphism have been implicated in the pathophysiology of major depressive disorder (MDD). We aimed to investigate the effects of genetic variants of the 5-HTTLPR and BDNF Val66Met polymorphisms and their interactions with MDD on cortical volume and white matter integrity. Ninety-five patients with MDD and 65 healthy participants aged 20-65 years were recruited. The subjects were genotyped for the 5-HTTLPR and BDNF Val66Met polymorphisms and scanned with T1-weighted and diffusion tensor imaging...
February 2, 2018: Psychiatry Research
https://www.readbyqxmd.com/read/29412172/in-vitro-pharmacology-of-ambroxol-potential-serotonergic-sites-of-action
#14
J D Hull, R A Lyon
AIMS: Ambroxol is a muco-active agent with multiple, clinically relevant effects in the airway. Despite its widespread use and well documented clinical efficacy, there are few data on its mechanism of action and receptor pharmacology beyond sodium channel blockade and inhibition of guanylate cyclase. Accordingly, in vitro studies were conducted to determine its overall receptor pharmacology and possible sites of action. MATERIALS AND METHODS: In vitro radioligand binding/enzyme inhibition studies were conducted at 62 receptors, ion channels and enzymes using standard techniques...
February 2, 2018: Life Sciences
https://www.readbyqxmd.com/read/29411704/-genetic-association-analyses-of-the-endocannabinoid-system-on-anxious-phenotype
#15
Judit Lazary, Nora Eszlari, Gabriella Juhasz, Gyorgy Bagdy
BACKGROUND: Accumulating data confirmed that the endocannabinoid system (ECS) is involved in the regulation of stress response and emotional processes, therefore ECS became an important pharmacological target as a potential anxiolytic. Although unequivocal data from animal studies confirmed the relevancy of the ECS in anxious phenotype, human genetic data are poorly available in the literature in this field. In the presented studies we tested possible associations between anxious phenotype and the cannabinoid receptor 1 and the fatty acid amide hydrolase gene polymorphisms...
December 2017: Neuropsychopharmacologia Hungarica
https://www.readbyqxmd.com/read/29407665/risky-driving-and-the-persistent-effect-of-a-randomized-intervention-focusing-on-impulsivity-the-role-of-the-serotonin-transporter-promoter-polymorphism
#16
Diva Eensoo, Marika Paaver, Mariliis Vaht, Helle-Mai Loit, Jaanus Harro
Road traffic accidents are a serious public health issue, and real-life traffic offences are an excellent indicator of the behavioural tendencies of impulsivity and risk-taking. We have previously reported on short-term efficacy of a brief intervention in driving schools to reduce traffic risks (Paaver et al., Accid. Anal. Prev., 2013; 50, 430-437), and have now addressed the question of whether does the impact of the intervention last for a few years, and whether traffic behaviour and the intervention effect are associated with the serotonin transporter polymorphism (5-HTTLPR) genotype as the central serotonin system is strongly associated with impulse control...
February 1, 2018: Accident; Analysis and Prevention
https://www.readbyqxmd.com/read/29407218/membrane-cholesterol-removal-and-replenishment-affect-rat-and-monkey-brain-monoamine-transporters
#17
Marc Morissette, Nicolas Morin, Claude Rouillard, Thérèse Di Paolo
The dopamine transporter (DAT) is abundantly expressed in the striatum where it removes extracellular dopamine into the cytosol of presynaptic nerve terminals. It is the target of drugs of abuse and antidepressants. There is a loss of the DAT in Parkinson's disease affecting release of levodopa implicated in levodopa-induced dyskinesias. This study investigated the effect of cholesterol on DAT, serotonin transporter (SERT) and vesicular monoamine transporter 2 (VMAT2) in monkey and rat brains in vitro. DAT protein levels measured by Western blot remained unchanged with in vitro methyl-β-cyclodextrin (MCD) incubations to remove membrane cholesterol or with incubations to increase membrane cholesterol content...
January 30, 2018: Neuropharmacology
https://www.readbyqxmd.com/read/29392233/regular-physical-activity-prevents-development-of-chronic-muscle-pain-through-modulation-of-supraspinal-opioid-and-serotonergic-mechanisms
#18
Renan G Brito, Lynn A Rasmussen, Kathleen A Sluka
Introduction: It is generally believed that exercise produces its effects by activating central opioid receptors; there are little data that support this claim. The periaqueductal gray (PAG) and rostral ventromedial medulla (RVM) are key nuclei in opioid-induced analgesia, and opioids interact with serotonin to produce analgesia. Objectives: The purpose was to examine central inhibitory mechanisms involved in analgesia produced by wheel running. Methods: C57/Black6 mice were given access to running wheels in their home cages before induction of chronic muscle hyperalgesia and compared with those without running wheels...
September 2017: Pain Reports (Baltimore, Md.)
https://www.readbyqxmd.com/read/29391756/effect-of-lactobacillus-rhamnosus-gg-supernatant-on-serotonin-transporter-expression-in-rats-with-post-infectious-irritable-bowel-syndrome
#19
Ya-Nan Cao, Li-Juan Feng, Yuan-Yuan Liu, Kui Jiang, Mao-Jun Zhang, Yi-Xin Gu, Bang-Mao Wang, Jia Gao, Ze-Lan Wang, Yu-Ming Wang
AIM: To evaluate the effect of Lactobacillus rhamnosus GG supernatant (LGG-s) on the expression of serotonin transporter (SERT) in rats with post-infectious irritable bowel syndrome (PI-IBS). METHODS: Campylobacter jejuni 81-176 (1010 CFU/mL) was used to induce intestinal infection to develop a PI-IBS model. After evaluation of the post-infectious phase by biochemical tests, DNA agarose gel electrophoresis, abdominal withdrawal reflex (AWR) test, and the intestinal motility test, four PI-IBS groups received different concentrations of LGG-s for 4 wk...
January 21, 2018: World Journal of Gastroenterology: WJG
https://www.readbyqxmd.com/read/29388323/effects-of-repeated-long-term-psychosocial-stress-and-acute-cannabinoid-exposure-on-mouse-corticostriatal-circuitries-implications-for-neuropsychiatric-disorders
#20
Jordi Tomas-Roig, Fabiana Piscitelli, Vanesa Gil, Ester Quintana, Lluís L Ramió-Torrentà, Jose Antonio Del Río, Timothy Patrick Moore, Hope Agbemenyah, Gabriela Salinas, Claudia Pommerenke, Stephan Lorenzen, Tim Beißbarth, Sigrid Hoyer-Fender, Vincenzo Di Marzo, Ursula Havemann-Reinecke
INTRODUCTION: Vulnerability to psychiatric manifestations is achieved by the influence of genetic and environment including stress and cannabis consumption. Here, we used a psychosocial stress model based on resident-intruder confrontations to study the brain corticostriatal-function, since deregulation of corticostriatal circuitries has been reported in many psychiatric disorders. CB1 receptors are widely expressed in the central nervous system and particularly, in both cortex and striatum brain structures...
January 31, 2018: CNS Neuroscience & Therapeutics
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