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https://www.readbyqxmd.com/read/29456627/growth-and-lipid-accumulation-by-different-nutrients-in-the-microalga-chlamydomonas-reinhardtii
#1
Lei Yang, Jun Chen, Shan Qin, Min Zeng, Yongguang Jiang, Lang Hu, Peng Xiao, Wenlong Hao, Zhangli Hu, Anping Lei, Jiangxin Wang
Background: Individual nutrient depletion is widely used to induce lipid accumulation in microalgae, which also causes cell growth inhibition and decreases the total biomass. Thus, improving the lipid accumulation without biomass loss in the nutrient deficiency cells becomes a potential cost-effective treatment for cheaper biofuels. Methods: In this study, the effects of different nutritional conditions on the growth and contents of lipids in Chlamydomonas reinhardtii were compared, and the metabolic profiles under different nutritional conditions were also investigated...
2018: Biotechnology for Biofuels
https://www.readbyqxmd.com/read/29456548/gaba-shunt-in-durum-wheat
#2
REVIEW
Petronia Carillo
Plant responses to salinity are complex, especially when combined with other stresses, and involve many changes in gene expression and metabolic fluxes. Until now, plant stress studies have been mainly dealt only with a single stress approach. However, plants exposed to multiple stresses at the same time, a combinatorial approach reflecting real-world scenarios, show tailored responses completely different from the response to the individual stresses, due to the stress-related plasticity of plant genome and to specific metabolic modifications...
2018: Frontiers in Plant Science
https://www.readbyqxmd.com/read/29455069/photo-crosslinkable-injectable-sericin-hydrogel-as-3d-biomimetic-extracellular-matrix-for-minimally-invasive-repairing-cartilage
#3
Chao Qi, Jia Liu, Yang Jin, Luming Xu, Guobin Wang, Zheng Wang, Lin Wang
Millions of patients worldwide suffer from cartilage injury and age/disease-related cartilage degeneration. However, cartilage, such as articular cartilage, is poor at self-regeneration. Current treatments are often invasive with limited efficacy. Developing minimal invasive strategies for effective cartilage repair is highly desired. Here, we report an injectable, photo-crosslinkable sericin hydrogel as a biomimetic extracellular matrix for minimal invasively repairing cartilage. Sericin was functionalized to be sericin methacryloyl (SerMA), which formed an in situ hydrogel upon UV light irradiation via photo-crosslinking...
February 9, 2018: Biomaterials
https://www.readbyqxmd.com/read/29453261/molecular-and-functional-study-of-a-branching-sucrase-like-glucansucrase-reveals-an-evolutionary-intermediate-between-two-subfamilies-of-the-gh70-enzymes
#4
Minghui Yan, Bing-Hua Wang, Xiaofen Xu, Peng Chang, Feng Hang, Zhengjun Wu, Chunping You, Zhenmin Liu
Family GH70 glucansucrases (GSs) catalyze the synthesis of α-glucans from sucrose, a reaction widely seen in lactic acid bacteria (LAB). These enzymes have been implicated in many aspects of the microbial life. Products of GSs have great commercial value as food supplements and medical materials, therefore these enzymes have attracted much attention from both fields of science and industry. However, certain issues concerning the origin and evolution of GS are still to be addressed, although an increasing number of GH70 enzymes have been characterized...
February 16, 2018: Applied and Environmental Microbiology
https://www.readbyqxmd.com/read/29452110/exploring-the-impact-of-house-screening-intervention-on-entomological-indices-and-incidence-of-malaria-in-arba-minch-town-southwest-ethiopia-a-randomized-control-trial
#5
Solomon Kinde Getawen, Temesgen Ashine, Fekadu Massebo, Daniel Woldeyes, Bernt Lindtjørn
House is the major site for malaria infection where most human-vector contact takes place. Hence, improving housing might reduce the risk of malaria infection by limiting house entry of vectors. This study aimed to explore the impact of screening doors and windows with wire meshes on density and entomological inoculation rate (EIR) of malaria vector, and malaria incidence, and assess the acceptability, durability, and cost of the intervention. The susceptibility status of malaria vector was also assessed. A two-arm randomized trial was done in Arba Minch Town, southwest Ethiopia...
February 13, 2018: Acta Tropica
https://www.readbyqxmd.com/read/29449599/red-blood-cells-are-dynamic-reservoirs-of-cytokines
#6
Elisabeth Karsten, Edmond Breen, Benjamin R Herbert
Red blood cells (RBCs) have been shown to affect immune function and can induce inflammatory responses after transfusion. The transfusion of washed RBCs can significantly reduce adverse effects, however, the soluble factors that may mediate these effects have not been identified. Previous studies have identified, but not quantified, a small number of chemokines associated with RBCs. We isolated RBCs from healthy volunteers and quantified of a panel of 48 cytokines, chemokines, and growth factors in the lysate, cytosol, and conditioned media of these cells using Luminex ® technology...
February 15, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29448811/production-of-a-new-mucilage-compound-in-lepidium-sativum-callus-by-optimizing-in-vitro-growth-conditions
#7
Pooran Golkar, Fatemeh Hadian, Mehrana Koohi Dehkordi
The mucilage in Lepidium sativum L. is considered a biologically active compound with diverse medicinal properties. Different explants (hypocotyls and leaf) were transferred to Murashige and Skoog (MS) medium supplemented with twelve different plant growth regulator combinations under two different incubations (light and dark). The best mucilage production from callus (36.76% g g -1 dry weight) was obtained in the MS medium supplemented with 1 mg L -1 of 2, 4-D and 2 mg L -1 of BAP under the light condition...
February 15, 2018: Natural Product Research
https://www.readbyqxmd.com/read/29448697/characterization-of-ddab-cholesterol-vesicles-and-its-comparison-with-lipid-cholesterol-vesicles
#8
Fumihiko Iwasaki, Keishi Suga, Yukihiro Okamoto, Hiroshi Umakoshi
Vesicles prepared by synthetic surfactant, DDAB (dilauryldimethylammonium bromide), were modified with cholesterol and their membrane surface properties of the vesicle were characterized through the analyses of fluorescent probes, such as Laurdan (6-lauroyl-2-dimethylaminonaphthalene) and DPH (1,6-diphenyl-1,3,5-hexatriene). The self-assembly of DDAB with cholesterol showed stable vesicle structure with a mean diameter of 127 nm through the dynamic light scattering analysis. While the DDAB vesicle showed high polarity and high fluidity, the modification of the DDAB vesicle with cholesterol lead to the formation of "heterogeneous phase" on the vesicle membrane...
March 1, 2018: Journal of Nanoscience and Nanotechnology
https://www.readbyqxmd.com/read/29447478/dendritic-platforms-for-biomimicry-and-biotechnological-applications
#9
Kalpana Nagpal, Anand Mohan, Sourav Thakur, Pradeep Kumar
Dendrimers, commonly referred to as polymeric trees, offer endless opportunities for biotechnological and biomedical applications. By controlling the type, length, and molecular weight of the core, branches and end groups, respectively, the chemical functionality and topology of dendrimeric archetypes can be customized which further can be applied to achieve required solubility, biodegradability, diagnosis and other applications. Given the physicochemical variability of the dendrimers and their hybrids, this review attempts to discuss a full spectrum of recent advances and strides made by these "perfectly designed structures"...
February 15, 2018: Artificial Cells, Nanomedicine, and Biotechnology
https://www.readbyqxmd.com/read/29447428/uv-b-radiation-delays-flowering-time-through-changes-in-the-prc2-complex-activity-and-mir156-levels-in-arabidopsis-thaliana
#10
Marcela Dotto, María Sol Gómez, María Soledad Soto, Paula Casati
UV-B is a high-energy component of the solar radiation perceived by the plant and induces a number of modifications in plant growth and development, including changes in flowering time. However, the molecular mechanisms underlying these changes are largely unknown. In the present work, we demonstrate that Arabidopsis plants grown under white light supplemented with UV-B show a delay in flowering time, and this developmental reprogramming is mediated by the UVR8 photoreceptor. Using a combination of gene expression analyses and UV-B irradiation of different flowering mutants, we gained insight into the pathways involved in the observed flowering time delay in UV-B exposed Arabidopsis plants...
February 15, 2018: Plant, Cell & Environment
https://www.readbyqxmd.com/read/29446620/polydopamine-coated-main-chain-liquid-crystal-elastomer-as-optically-driven-artificial-muscle
#11
Hongmiao Tian, Zhijian Wang, Yilong Chen, Jinyou Shao, Tong Gao, Shengqiang Cai
Optically-driven active materials have received much attention because their deformation and motion can be controlled remotely, instantly and precisely in a contactless way. In this article, we investigated an optically-actuated elastomer with rapid response: polydopamine (PDA)-coated liquid crystal elastomer (LCE). Due to the photothermal effect of PDA coating and thermal responsiveness of LCE, the elastomer film contracted significantly with near infrared (NIR) irradiation. With a fixed strain, light-induced actuating stress in the film could be as large as 1...
February 15, 2018: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/29444675/current-and-future-distribution-of-aedes-aegypti-and-aedes-albopictus-diptera-culicidae-in-who-eastern-mediterranean-region
#12
Els Ducheyne, Nhu Nguyen Tran Minh, Nabil Haddad, Ward Bryssinckx, Evans Buliva, Frédéric Simard, Mamunur Rahman Malik, Johannes Charlier, Valérie De Waele, Osama Mahmoud, Muhammad Mukhtar, Ali Bouattour, Abdulhafid Hussain, Guy Hendrickx, David Roiz
BACKGROUND: Aedes-borne diseases as dengue, zika, chikungunya and yellow fever are an emerging problem worldwide, being transmitted by Aedes aegypti and Aedes albopictus. Lack of up to date information about the distribution of Aedes species hampers surveillance and control. Global databases have been compiled but these did not capture data in the WHO Eastern Mediterranean Region (EMR), and any models built using these datasets fail to identify highly suitable areas where one or both species may occur...
February 14, 2018: International Journal of Health Geographics
https://www.readbyqxmd.com/read/29444322/physiological-reactions-to-long-term-fishing-in-the-barents-sea
#13
B Netterstrøm, Å M Hansen, D Isaacson, A M Simonsen, P Weihe
Background: Fishing in distant waters for months may induce physiological stress. Aims: To assess the physiological stress reactions in fishermen working for 2-3 months continuously in 6-h shifts on trawlers in the Barents Sea. Methods: The crew of five trawlers fishing in the Barents Sea from January to April 2004 were invited to participate. In the week before and 5-7 days after the trip, the following measures were collected: salivary cortisol four times a day, 24-h urinary cortisol, blood pressure, heart rate, serum cholesterol, serum high-density lipoprotein (HDL-cholesterol), HbA1c (glycosylated haemoglobin) and weight...
February 10, 2018: Occupational Medicine
https://www.readbyqxmd.com/read/29443990/photonic-hook-a-new-curved-light-beam
#14
Liyang Yue, Oleg V Minin, Zengbo Wang, James N Monks, Alexander S Shalin, Igor V Minin
It is well known that electromagnetic radiation propagates along a straight line, but this common sense was broken by the artificial curved light-the Airy beam. In this Letter, we demonstrate a new type of curved light beam besides the Airy beam, the so-called "photonic hook." This photonic hook is a curved high-intensity focus by a dielectric trapezoid particle illuminated by a plane wave. The difference between the phase velocity and the interference of the waves inside the particle causes the phenomenon of focus bending...
February 15, 2018: Optics Letters
https://www.readbyqxmd.com/read/29439710/semet-attenuates-ota-induced-pcv2-replication-promotion-by-inhibiting-autophagy-by-activating-the-akt-mtor-signaling-pathway
#15
Gang Qian, Dandan Liu, Junfa Hu, Fang Gan, Lili Hou, Nianhui Zhai, Xingxiang Chen, Kehe Huang
Porcine circovirus type 2 (PCV2) is recognized as the causative agent of porcine circovirus-associated diseases. PCV2 replication could be promoted by low doses of ochratoxin A (OTA) as in our previous study and selenium has been shown to attenuate PCV2 replication. However, the underlying mechanism remains unclear. The aim of the study was to investigate the effects of selenomethionine (SeMet), the major component of organic selenium, on OTA-induced PCV2 replication promotion and its potential mechanism. The present study demonstrates that OTA could promote PCV2 replication as measured by cap protein expression, viral titer, viral DNA copies and the number of infected cells...
February 13, 2018: Veterinary Research
https://www.readbyqxmd.com/read/29439569/thylakoid-containing-artificial-cells-for-the-inhibition-investigation-of-light-driven-electron-transfer-during-photosynthesis
#16
Wei Zong, Xunan Zhang, Chao Li, Xiaojun Han
The fabrication of artificial cells containing nature components is challenging. Herein we construct a thylakoid containing artificial cell (TA-cell) by forming multicompartmental structure inside giant unilamellar vesicles (GUVs) using osmotic stress. The thylakoids are selectively loaded inside each compartment to mimic "chloroplast". The TA-cells are able to carry out photosynthesis upon light on. The TA-cells keep their 50% functionality of electron transfer for 12 days, which is twice of those of free thylakoids...
February 13, 2018: ACS Synthetic Biology
https://www.readbyqxmd.com/read/29439339/plasma-antioxidant-status-in-patients-with-alzheimer-s-disease-and-cognitively-intact-elderly-a-meta-analysis-of-case-control-studies
#17
Kathryn Mullan, Chris R Cardwell, Bernadette McGuinness, Jayne V Woodside, Gareth J McKay
Serum antioxidants may afford neuroprotection against Alzheimer's disease (AD) via correction of the pro-oxidative imbalance but findings reported have been inconsistent. We compared the pooled mean difference in serum levels of ten dietary antioxidants between patients with AD and cognitively intact controls from 52 studies in meta-analyses using random-effects models. Patients with AD had significantly lower plasma levels of α-carotene, β-carotene, lycopene, lutein, vitamin A, C, and E, and uric acid. No significant difference was observed for plasma levels of β-cryptoxanthin and zeaxanthin...
2018: Journal of Alzheimer's Disease: JAD
https://www.readbyqxmd.com/read/29439091/evaluation-of-galectin-1-and-galectin-3-as-prospective-biomarkers-in-keratoconus
#18
Frans Eberth Costa Andrade, Joyce Luciana Covre, Lisandra Ramos, Rossen Mihaylov Hazarbassanov, Myrna Serapião Dos Santos, Mauro Campos, José Álvaro Pereira Gomes, Cristiane Damas Gil
AIMS: To evaluate the expression of β-galactoside-binding proteins galectin (Gal)-1 and Gal-3 in patients with keratoconus (KC) and postcorneal collagen cross-linking (CXL) treatment in vitro. METHODS: Tear fluid, cornea samples and conjunctival impression cytology specimens from control and KC patients were used to evaluate Gal-1 and Gal-3 expressions. Primary keratocytes were isolated by collagenase digestion from surgically removed corneas of five normal or KC human corneal buttons and cultured in Dulbecco's modified eagle medium/Ham's F12 medium supplemented with 2% fetal bovine serum...
February 8, 2018: British Journal of Ophthalmology
https://www.readbyqxmd.com/read/29437989/galactoglycerolipid-lipase-pgd1-is-involved-in-thylakoid-membrane-remodeling-in-response-to-adverse-environmental-conditions-in-chlamydomonas
#19
Zhi-Yan Du, Ben Franklin Lucker, Krzysztof Zienkiewicz, Tarryn E Miller, Agnieszka Zienkiewicz, Barbara B Sears, David M Kramer, Christoph Benning
Photosynthesis occurs in the thylakoid membrane, where the predominant lipid is monogalactosyldiacylglycerol (MGDG). As environmental conditions change, photosynthetic membranes have to adjust. In this study, we used a loss-of-function Chlamydomonas reinhardtii mutant deficient in the MGDG-specific lipase PGD1 (PLASTID GALACTOGLYCEROLIPID DEGRADATION1) to investigate the link between MGDG turnover, chloroplast ultrastructure, and the production of reactive oxygen species (ROS) in response to different adverse environmental conditions...
February 5, 2018: Plant Cell
https://www.readbyqxmd.com/read/29436719/artificial-selection-sheds-light-on-developmental-mechanisms-of-limb-elongation
#20
Marta Marchini, Campbell Rolian
Species diversity in limb lengths and proportions is thought to have evolved adaptively in the context of locomotor and habitat specialization, but the heritable cellular processes that drove this evolution within species are poorly understood. In this study, we take a novel "micro-evo-devo" approach, using artificial selection on relative limb length to amplify phenotypic variation in a population of mice, known as Longshanks, to examine the cellular mechanisms of postnatal limb development that contribute to intraspecific limb length variation...
February 13, 2018: Evolution; International Journal of Organic Evolution
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