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https://www.readbyqxmd.com/read/28937651/perennial-grass-and-native-wildflowers-a-synergistic-approach-to-habitat-management
#1
Shereen S Xavier, Dawn M Olson, Alisa W Coffin, Timothy C Strickland, Jason M Schmidt
Marginal agricultural land provides opportunities to diversify landscapes by producing biomass for biofuel, and through floral provisioning that enhances arthropod-mediated ecosystem service delivery. We examined the effects of local spatial context (adjacent to woodland or agriculture) and irrigation (irrigation or no irrigation) on wildflower bloom and visitation by arthropods in a biofeedstocks-wildflower habitat buffer design. Twenty habitat buffer plots were established containing a subplot of Napier grass (Pennisetum perpureum Schumach) for biofeedstock, three commercial wildflower mix subplots, and a control subplot containing spontaneous weeds...
September 22, 2017: Insects
https://www.readbyqxmd.com/read/28937649/synthesis-and-enhanced-ethanol-gas-sensing-properties-of-the-g-c%C3%A2-n%C3%A2-nanosheets-decorated-tin-oxide-flower-like-nanorods-composite
#2
Yan Wang, Jianliang Cao, Cong Qin, Bo Zhang, Guang Sun, Zhanying Zhang
Flower-like SnO₂/g-C₃N₄ nanocomposites were synthesized via a facile hydrothermal method by using SnCl₄·5H₂O and urea as the precursor. The structure and morphology of the as-synthesized samples were characterized by using the X-ray powder diffraction (XRD), electron microscopy (FESEM and TEM), and Fourier transform infrared spectrometer (FT-IR) techniques. SnO₂ displays the unique 3D flower-like microstructure assembled with many uniform nanorods with the lengths and diameters of about 400-600 nm and 50-100 nm, respectively...
September 22, 2017: Nanomaterials
https://www.readbyqxmd.com/read/28936847/-analysis-and-evaluation-of-alkaloids-and-flavonoids-in-flower-of-sophora-flavescens-from-shanxi-province
#3
Huang-Qin Zhang, Zhen-Hua Zhu, Da-Wei Qian, Ze-Bin Weng, Sheng Guo, Jin-Ao Duan, Zhen-Hong Lei, An-Ping Li
This study intends to explore the potential resource-orientedutilization value of the flower of Sophora flavescents by analyzing alkaloids and flavonoids in the flower of S. flavescens from Shanxi province. This study established a rapid UPLC-TQ-MS/MS method that is used for determination of seven alkaloids and seven flavonoids in the flower of S.flavescens. The different florescences all have the seven detected alkaloids such as cytisine, oxy-matrine, oxy-sophocarpine, sophoridine, N-methylcytisine, matrine, sophocarpine...
December 2016: Zhongguo Zhong Yao za Zhi, Zhongguo Zhongyao Zazhi, China Journal of Chinese Materia Medica
https://www.readbyqxmd.com/read/28936706/biodiversity-and-ecology-of-flower-associated-actinomycetes-in-different-flowering-stages-of-protea-repens
#4
Zander R Human, Casparus J Crous, Francois Roets, Stephanus N Venter, Michael J Wingfield, Z Wilhelm de Beer
Actinomycete bacteria have previously been reported from reproductive structures (infructescences) of Protea (sugarbush/suikerbos) species, a niche dominated by fungi in the genera Knoxdaviesia and Sporothrix. It is probable that these taxa have symbiotic interactions, but a lack of knowledge regarding their diversity and general ecology precludes their study. We determined the diversity of actinomycetes within Protea repens inflorescence buds, open inflorescences, young and mature infructescences, and leaf litter surrounding these trees...
September 21, 2017: Antonie Van Leeuwenhoek
https://www.readbyqxmd.com/read/28936666/identification-of-cis-regulatory-modules-that-function-in-the-male-germline-of-flowering-plants
#5
Benjamin Peters, Jack Aidley, Murray Cadzow, David Twell, Lynette Brownfield
The male germline of flowering plants develops within the vegetative cell of the male gametophyte and displays a distinct transcriptional profile. Key to understanding the development of this unique cell lineage is determining how gene expression is regulated within germline cells. This knowledge impacts upon our understanding of cell specification, differentiation, and plant fertility. Here, we describe methods to identify cis-regulatory modules (CRMs) that act as key regulatory regions in the promoters of germline-expressed genes...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28936665/phosphoprotein-enrichment-from-tobacco-mature-pollen-crude-protein-extract
#6
Jan Fíla, David Honys
Protein phosphorylation was repeatedly shown to be the most dynamic post-translational modification mediated by a huge orchestra of protein kinases and phosphatases. Upon landing on a stigma, pollen grain dehydration and activation are accompanied by changes in protein phosphorylation together with the translation activation of stored mRNAs. To enable studies of the total phosphoproteome, it is usually necessary to apply various enrichment techniques. In this chapter, one of these protocols that worked previously well on tobacco mature pollen is presented in more detail...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28936662/manual-isolation-of-living-cells-from-the-arabidopsis-thaliana-female-gametophyte-by-micromanipulation
#7
Maria Englhart, Lucija Šoljić, Stefanie Sprunck
The few-celled female gametophyte, or embryo sac, of flowering plants is not easily accessible as it is buried within the sporophytic tissues of the ovule. Nevertheless, it has become an attractive model system to study the molecular mechanisms underlying patterning and cell type specification, as well as fertilization of the two female gametes, the egg and the central cell. While female gametes, zygotes, and early embryos can be manually isolated from the embryo sacs in maize, wheat, tobacco, and rice by micromanipulation, this approach had been considered impossible for the much smaller embryo sac of the model plant Arabidopsis thaliana...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28936661/isolation-of-rice-sperm-cells-for-transcriptional-profiling
#8
Scott D Russell, Daniel S Jones, Sarah Anderson, Xinkun Wang, Venkatesan Sundaresan, Xiaoping Gou
The male germline of flowering plants displays unexpectedly divergent transcriptional profiles compared to other cell types and tissues of plants. As these are among the smallest cells, and are harbored within pollen, isolating a pure collection of germline RNA presents unusual challenges. The sperm cells of rice represent a particularly challenging subject for study as the pollen are unusually short lived upon release from the anther, and the marker gene sequences that make FACS possible in Arabidopsis have not yet been introduced into rice...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28936655/immunolocalization-of-agps-and-pectins-in-quercus-suber-gametophytic-structures
#9
Mário Luís da Costa, Ana Lúcia Lopes, Maria Isabel Amorim, Sílvia Coimbra
The arabinogalactan proteins (AGPs) are highly glycosylated proteins, ubiquitous in plants that have been linked to numerous aspects of sexual reproduction in several plant species, including the monoecious tree species Quercus suber. AGPs are found in cell membranes and cell walls of all types of tissues, including reproductive cells and organs. Pectins are cell wall components that also have been shown to change in composition and quantity during the maturations of the male and female gametophyte in cork oak...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28936653/live-cell-imaging-of-auxin-and-cytokinin-signaling-in-maize-female-gametophytes
#10
Antony M Chettoor, Matthew M S Evans
The plant life cycle is characterized by the alternation of generations between genetically active diploid sporophytes and haploid gametophytes. The gametophytes of flowering plants are sexually dimorphic. While the male gametophyte consists of only three cells (two sperm and a vegetative cell) and is released by the parent sporophyte, the female gametophyte (or embryo sac) is more complex and remains imbedded within diploid sporophyte tissues. In maize, the female gametophyte is embedded in a large ovule surrounded with multiple nucellar cell layers impeding live-cell imaging approaches to study embryo sac functions...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28936650/analysis-of-fluorescent-reporter-activity-in-the-male-germline-during-pollen-development-by-confocal-microscopy
#11
David Twell, Lynette Brownfield
The male germline of flowering plants develops within the vegetative cell of the male gametophyte (pollen). The germline is established by asymmetric division of the microspore to form the generative cell. Mitotic division of the generative cell then produces the two sperm cells required for double fertilization. These differentiate to produce the proteins required for gamete attachment and fusion. An important aspect of understanding germline development is the characterization of germline gene expression...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28936647/3d-imaging-of-whole-mount-ovules-at-cellular-resolution-to-study-female-germline-development-in-rice
#12
Ethel Mendocilla-Sato, Wenjing She, Célia Baroux
Recent advances in fluorescence-based staining of cellular compartments coupled with confocal microscopy imaging have allowed the visualization of three-dimensional (3D) structures with cellular resolution in various intact plant tissues and species. Such approaches are of particular interest for the analysis of the reproductive lineage in plants including the meiotic precursor cells deeply embedded within the ovary of the gynoecium enclosed in the flower. Yet, their relative inaccessibility and the lack of optical clarity of plant tissues prevent robust staining and imaging across several cell layers...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28936220/de-novo-assembly-of-the-camellia-nitidissima-transcriptome-reveals-key-genes-of-flower-pigment-biosynthesis
#13
Xingwen Zhou, Jiyuan Li, Yulin Zhu, Sui Ni, Jinling Chen, Xiaojuan Feng, Yunfeng Zhang, Shuangquan Li, Hongguang Zhu, Yuanguang Wen
The golden camellia, Camellia nitidissima Chi., is a well-known ornamental plant that is known as "the queen of camellias" because of its golden yellow flowers. The principal pigments in the flowers are carotenoids and flavonol glycosides. Understanding the biosynthesis of the golden color and its regulation is important in camellia breeding. To obtain a comprehensive understanding of flower development in C. nitidissima, a number of cDNA libraries were independently constructed during flower development. Using the Illumina Hiseq2500 platform, approximately 71...
2017: Frontiers in Plant Science
https://www.readbyqxmd.com/read/28935951/overexpression-of-annexin-gene-annsp2-enhances-drought-and-salt-tolerance-through-modulation-of-aba-synthesis-and-scavenging-ros-in-tomato
#14
Raina Ijaz, Javeria Ejaz, Shenghua Gao, Tengfei Liu, Muhammad Imtiaz, Zhibiao Ye, Taotao Wang
Drought and high salinity are two major abiotic stresses that significantly affect agricultural crop productivity worldwide. Annexins are a multigene family that plays an essential role in plant stress responses and various cellular processes. Here, the AnnSp2 gene was cloned from drought-resistant wild tomato (Solanum pennellii) and functionally characterized in cultivated tomato. AnnSp2 protein was localized in the nucleus and had higher expression in leave, flower and fruit. It was induced by several phytohormones and some abiotic stresses...
September 21, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28935204/the-taishan-robinia-pseudoacacia-polysaccharides-enhance-immune-effects-of-rabbit-haemorrhagic-disease-virus-inactivated-vaccines
#15
Shifa Yang, Guiming Li, Zengcheng Zhao, Minyan Feng, Jian Fu, Zhongli Huang, Minxun Song, Shuqian Lin
Robinia pseudoacacia flower, a common component in traditional Chinese medicine, has long been well-known for its high pharmaceutical value. This study aimed to assess the immunopotentiating effects of Taishan Robinia Pseudoacacia polysaccharides (TRPPS) in rabbits inoculated with a rabbit haemorrhagic disease virus (RHDV) inactivated vaccine. The rabbits were administered with the RHDV vaccine in conjunction with varying concentrations of TRPPS, and their blood samples were collected at different time points to analyze the ratio and number of blood lymphocytes...
September 18, 2017: Microbial Pathogenesis
https://www.readbyqxmd.com/read/28934913/flavonoid-compounds-related-to-seed-coat-color-of-wheat
#16
Noriko Kohyama, Makiko Chono, Hiroyuki Nakagawa, Yosuke Matsuo, Hiroshi Ono, Hitoshi Matsunaka
In red wheat, reddish-brown pigments accumulate in testa of mature seeds. Half-cut wheat seeds were immersed in p-dimethylaminocinnamaldehyde (DMACA) reagent that stains flavanol structures blue. Testa of 10-40 days after flowering (DAF) in red wheat ("Norin 61" and "Satonosora") seeds were stained blue and the reagent color changed to blue with 10-25 DAF seeds. No blue staining was observed in white wheat ("Tamaizumi") seeds during maturation. "Norin 61" seed coats at 10 DAF contained dihydroquercetin, dihydromyricetin, (+)-catechin, procyanidin B3, and prodelphinidin B3, which were identified by HPLC-diode array detector and LC-MS/MS analyses...
September 22, 2017: Bioscience, Biotechnology, and Biochemistry
https://www.readbyqxmd.com/read/28934701/hydrothermal-synthesis-of-4zno%C3%A2-b2o3%C3%A2-h2o-ln-3-ln-eu-tb-phosphors-morphology-tunable-and-luminescence-properties
#17
Shiwei Cao, Yang Jiao, Weifang Han, Chunhua Ge, Bo Song, Jie Wang, Xiangdong Zhang
4ZnO·B2O3·H2O:Ln(3+) (Ln=Eu, Tb) phosphors with different morphologies have been successfully synthesized via one-step hydrothermal method through regulating the molar amount of Eu(3+) and Tb(3+). Comprehensive scanning electron microscopy (SEM), X-ray diffraction (XRD) Fourier transform infrared spectrum (FT-IR) and inductively coupled plasma atomic emission spectrometer (ICP-AES) characterizations all confirm that obtained products are 4ZnO·B2O3·H2O:Ln(3+) (Ln=Eu, Tb). The experimental results displayed that the morphology and photoluminescence of compounds is regularly changed with increased the molar amount of rare earth ions...
September 18, 2017: Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy
https://www.readbyqxmd.com/read/28934240/microbial-characterization-of-bee-pollen-from-the-vesuvius-area-collected-by-using-three-different-traps
#18
Gianluigi Mauriello, Annachiara De Prisco, Gennaro Di Prisco, Antonietta La Storia, Emilio Caprio
Flower pollen is collected by honeybee foragers, adhered on their rear legs and transported into the hives in the form of pellets. Once in the hives, bee pollen is moisturised with nectar and bee mouth secretions and due to enzymatically modifications it becomes the so-called bee-bread, the protein reservoir of young bees. Bee pollen can be artificially removed from bee legs and collected by using specific systems, the bee pollen traps. Bee pollen is commercialized for human consumption as fresh product and after freezing or drying...
2017: PloS One
https://www.readbyqxmd.com/read/28934034/transfiguring-light-and-symphonic-blooming-a-wabi-sabi-story-of-humanbecoming-and-human-caring
#19
Lois S Kelley
Transfiguring Light and Symphonic Blooming is word-art to describe the flowering of the humanbecoming and human caring schools of thought over time. A Wabi Sabi story journey was created to turn the tide in a world where the building of a unique body of knowledge for nursing has fallen into disfavor with nursing itself. Using nature-aligned paintings and a Japanese story-art aesthetic with poetry, the beauty of falling away to unitary essentials and holding the value of "both-and" in unitary consciousness was illuminated...
October 2017: Nursing Science Quarterly
https://www.readbyqxmd.com/read/28933100/-decipherment-of-ancient-literature-about-danshen
#20
Ai-Ping Deng, Lan-Ping Guo, Zhi-Lai Zhan, Lu-Qi Huang
Danshen has a long history in China as a commen traditional Chinese medicine, but many other members of the Salvia family is also used as Danshen in particular regions. The name of Danshen were analyzed according to the color, efficacity and etc. To investigate the real origin of Danshen, the stalk, leaf, flower and radix in chronicle and pictures of ancient literature were deciphered. It showed that in the past dynasties, Salvia miltiorrhiza was the most likely plant used as Danshen. The rigin and quality evaluation of Danshen were also summerized, provided new insight and proof for the development and utilization of Danshen...
November 2016: Zhongguo Zhong Yao za Zhi, Zhongguo Zhongyao Zazhi, China Journal of Chinese Materia Medica
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