keyword
MENU ▼
Read by QxMD icon Read
search

SRPK

keyword
https://www.readbyqxmd.com/read/27739913/metabolic-interplay-between-cytosolic-phosphoenolpyruvate-carboxylase-and-mitochondrial-alternative-oxidase-in-thermogenic-skunk-cabbage-symplocarpus-renifolius
#1
Md Abu Sayed, Yui Umekawa, Kikukatsu Ito
Skunk cabbage (Symplocarpus renifolius) blooms in early spring and its inflorescence, referred to as the spadix, can produce enough heat to melt snow. Here, we investigated glycolytic carbon flow at the PEP branch-point in thermogenic spadices. Our analyses revealed that petals and pistils in thermogenic florets exhibited higher expression of SrPEPC and SrAOX transcripts than those of SrPK, SrPEPCK, and SrPEPtase. Moreover, enzymatic analyses showed high activities of PEPC in the extracts from thermogenic florets...
October 14, 2016: Plant Signaling & Behavior
https://www.readbyqxmd.com/read/26244849/potential-antileukemia-effect-and-structural-analyses-of-srpk-inhibition-by-n-2-piperidin-1-yl-5-trifluoromethyl-phenyl-isonicotinamide-srpin340
#2
Raoni Pais Siqueira, Éverton de Almeida Alves Barbosa, Marcelo Depólo Polêto, Germanna Lima Righetto, Thiago Vargas Seraphim, Rafael Locatelli Salgado, Joana Gasperazzo Ferreira, Marcus Vinícius de Andrade Barros, Leandro Licursi de Oliveira, Angelo Brunelli Albertoni Laranjeira, Márcia Rogéria Almeida, Abelardo Silva Júnior, Juliana Lopes Rangel Fietto, Jörg Kobarg, Eduardo Basílio de Oliveira, Robson Ricardo Teixeira, Júlio César Borges, Jose Andrés Yunes, Gustavo Costa Bressan
Dysregulation of pre-mRNA splicing machinery activity has been related to the biogenesis of several diseases. The serine/arginine-rich protein kinase family (SRPKs) plays a critical role in regulating pre-mRNA splicing events through the extensive phosphorylation of splicing factors from the family of serine/arginine-rich proteins (SR proteins). Previous investigations have described the overexpression of SRPK1 and SRPK2 in leukemia and other cancer types, suggesting that they would be useful targets for developing novel antitumor strategies...
2015: PloS One
https://www.readbyqxmd.com/read/25229152/a-theoretical-study-of-srpk-interaction-with-the-flexible-domains-of-hepatitis-b-capsids
#3
Jehoon Kim, Jianzhong Wu
Hepatitis B virus (HBV) controls genome encapsidation and reverse transcription from a single-stranded RNA to a double-stranded DNA through the flexible C-terminal domain (CTD) of the capsid proteins. Although the microscopic structure of the nucleocapsid plays a critical role in the life cycle of HBV, the location of CTD residues at different stages of viral replication remains poorly understood. In this work, we report the radial distributions of individual amino-acid residues of the CTD tails for both empty and RNA-containing HBV capsids by using a coarse-grained model for the key biological components and the classical density functional theory...
September 16, 2014: Biophysical Journal
https://www.readbyqxmd.com/read/25187541/induction-of-viral-7-methyl-guanosine-cap-independent-translation-and-oncolysis-by-mitogen-activated-protein-kinase-interacting-kinase-mediated-effects-on-the-serine-arginine-rich-protein-kinase
#4
Michael C Brown, Jeffrey D Bryant, Elena Y Dobrikova, Mayya Shveygert, Shelton S Bradrick, Vidyalakshmi Chandramohan, Darell D Bigner, Matthias Gromeier
UNLABELLED: Protein synthesis, the most energy-consuming process in cells, responds to changing physiologic priorities, e.g., upon mitogen- or stress-induced adaptations signaled through the mitogen-activated protein kinases (MAPKs). The prevailing status of protein synthesis machinery is a viral pathogenesis factor, particularly for plus-strand RNA viruses, where immediate translation of incoming viral RNAs shapes host-virus interactions. In this study, we unraveled signaling pathways centered on the ERK1/2 and p38α MAPK-interacting kinases MNK1/2 and their role in controlling 7-methyl-guanosine (m(7)G) "cap"-independent translation at enterovirus type 1 internal ribosomal entry sites (IRESs)...
November 2014: Journal of Virology
https://www.readbyqxmd.com/read/25187540/mitogen-activated-protein-kinase-interacting-kinase-regulates-mtor-akt-signaling-and-controls-the-serine-arginine-rich-protein-kinase-responsive-type-1-internal-ribosome-entry-site-mediated-translation-and-viral-oncolysis
#5
Michael C Brown, Mikhail I Dobrikov, Matthias Gromeier
UNLABELLED: Translation machinery is a major recipient of the principal mitogenic signaling networks involving Raf-ERK1/2 and phosphoinositol 3-kinase (PI3K)-mechanistic target of rapamycin (mTOR). Picornavirus internal ribosomal entry site (IRES)-mediated translation and cytopathogenic effects are susceptible to the status of such signaling cascades in host cells. We determined that tumor-specific cytotoxicity of the poliovirus/rhinovirus chimera PVSRIPO is facilitated by Raf-ERK1/2 signals to the mitogen-activated protein kinase (MAPK)-interacting kinase (MNK) and its effects on the partitioning/activity of the Ser/Arg (SR)-rich protein kinase (SRPK) (M...
November 2014: Journal of Virology
https://www.readbyqxmd.com/read/25007957/mir-9-regulates-the-post-transcriptional-level-of-vegf165a-by-targeting-srpk-1-in-arpe-19-cells
#6
Changshin Yoon, Daejin Kim, Seonghan Kim, Ga Bin Park, Dae Young Hur, Jae Wook Yang, Sae Gwang Park, Yeong Seok Kim
PURPOSE: To investigate the effect of the overexpression of miRNA-9 to the ratio of pro- and anti-angiogenic isoforms of vascular endothelial growth factor (VEGF) in human retinal pigment cells (ARPE-19). METHODS: Oxidative stress was induced to ARPE-19 cells by 4-hydroxynonenal (4-HNE), tert-butyl hydroperoxide (t-BH), and hypoxia chamber with 1% O₂. Expression patterns of miRNAs were validated by qPCR. Relative mRNA levels of VEGF and PEDF were measured by semi-quantitative PCR...
September 2014: Graefe's Archive for Clinical and Experimental Ophthalmology
https://www.readbyqxmd.com/read/24984036/recruiting-a-silent-partner-for-activation-of-the-protein-kinase-srpk1
#7
Brandon E Aubol, Joseph A Adams
The SRPK family of protein kinases regulates mRNA splicing by phosphorylating an essential group of factors known as SR proteins, so named for a C-terminal domain enriched in arginine-serine dipeptide repeats (RS domains). SRPKs phosphorylate RS domains at numerous sites altering SR protein subcellular localization and splicing function. The RS domains in these splicing factors differ considerably in overall length and dipeptide layout. Despite their importance, little is known about how these diverse RS domains interact with SRPKs and regulate SR protein phosphorylation...
July 22, 2014: Biochemistry
https://www.readbyqxmd.com/read/23887803/topical-antiangiogenic-srpk1-inhibitors-reduce-choroidal-neovascularization-in-rodent-models-of-exudative-amd
#8
COMPARATIVE STUDY
Melissa V Gammons, Oleg Fedorov, David Ivison, Chunyun Du, Tamsyn Clark, Claire Hopkins, Masatoshi Hagiwara, Andrew D Dick, Russell Cox, Steven J Harper, Jules C Hancox, Stefan Knapp, David O Bates
PURPOSE: Exudative AMD (wet AMD) is treated by monthly injection into the eye of anti-VEGF proteins. VEGF is alternatively spliced to produce numerous isoforms that differ in angiogenic activity. Serine-rich protein kinase-1 (SRPK1) has been identified as a regulator of pro-angiogenic VEGF splicing by phosphorylating serine-rich splicing factor-1 (SRSF1), which binds to VEGF pre-mRNA. We tested the hypothesis that topical (eye drop) SRPK1-selective inhibitors could be generated that reduce pro-angiogenic isoforms, and prevent choroidal neovascularization in vivo...
September 5, 2013: Investigative Ophthalmology & Visual Science
https://www.readbyqxmd.com/read/23828492/serine-arginine-protein-kinases-new-players-in-neurodegenerative-diseases
#9
REVIEW
Chi Bun Chan, Keqiang Ye
Serine-arginine protein kinases (SRPKs) are a group of serine kinases that recognize and phosphorylate protein substrates with serine-arginine dipeptide repeats. They are mainly involved in regulating pre-mRNA splicing via phosphorylating splicing factors, such as ASF/SF2 and SC35. Nevertheless, the functions of SRPKs in the nervous system are sketchy, although the kinases have significant expression in neurons. Our recent studies demonstrate that one of the SRPK members, SRPK2, participates in the neuronal survival, cell cycle progression, and memory determination in Alzheimer's disease...
2013: Reviews in the Neurosciences
https://www.readbyqxmd.com/read/23761094/srpk1-inhibition-modulates-vegf-splicing-to-reduce-pathological-neovascularization-in-a-rat-model-of-retinopathy-of-prematurity
#10
Melissa V R Gammons, Andrew D Dick, Steven J Harper, David O Bates
PURPOSE: We tested the hypothesis that recombinant human VEGF-A165b and the serine arginine protein kinase (SRPK) inhibitor, SRPIN340, which controls splicing of the VEGF-A pre-mRNA, prevent neovascularization in a rodent model of retinopathy of prematurity (ROP). METHODS: In the 50/10 oxygen-induced retinopathy (50/10 OIR) model that exposes newborn rats to repeated cycles of 24 hours of 50% oxygen alternating with 24 hours of 10% oxygen, pups received intraocular injections of SRPIN340, vehicle, VEGF165b, anti-VEGF antibody, or saline...
August 27, 2013: Investigative Ophthalmology & Visual Science
https://www.readbyqxmd.com/read/23707382/partitioning-rs-domain-phosphorylation-in-an-sr-protein-through-the-clk-and-srpk-protein-kinases
#11
Brandon E Aubol, Ryan M Plocinik, Jonathan C Hagopian, Chen-Ting Ma, Maria L McGlone, Reeti Bandyopadhyay, Xiang-Dong Fu, Joseph A Adams
SR proteins are essential splicing factors whose biological function is regulated through phosphorylation of their C-terminal RS domains. Prior studies have shown that cytoplasmic-nuclear translocalization of the SR protein SRSF1 is regulated by multisite phosphorylation of a long Arg-Ser repeat in the N-terminus of the RS domain while subnuclear localization is controlled by phosphorylation of a shorter Arg-Ser repeat along with several Ser-Pro dipeptides in the C-terminus of the RS domain. To better understand how these two kinases partition Arg-Ser versus Ser-Pro specificities, we monitored the phosphorylation of SRSF1 by CLK1 and SRPK1...
August 23, 2013: Journal of Molecular Biology
https://www.readbyqxmd.com/read/23559848/specific-inhibition-of-serine-arginine-rich-protein-kinase-attenuates-choroidal-neovascularization
#12
Zhenyu Dong, Kousuke Noda, Atsuhiro Kanda, Junichi Fukuhara, Ryo Ando, Miyuki Murata, Wataru Saito, Masatoshi Hagiwara, Susumu Ishida
PURPOSE: To investigate the applicability of serine/arginine-rich protein kinase (SRPK)-specific inhibitor, SRPIN340, for attenuation of choroidal neovascularization (CNV) formation using a mouse model. METHODS: Laser photocoagulation was performed to induce CNV in C57BL/6J mice, followed by intravitreal injection of SRPIN340 or vehicle. Seven days after the treatment, the CNV size was evaluated using a flatmount technique. Protein levels of vascular endothelial growth factor (VEGF) and inflammation-associated molecules, such as monocyte chemoattractant protein (MCP)-1 and intercellular adhesion molecule (ICAM)-1, in the retinal pigment epithelium-choroid complex were measured with enzyme-linked immunosorbent assay...
2013: Molecular Vision
https://www.readbyqxmd.com/read/23505451/functional-analysis-of-the-aspergillus-nidulans-kinome
#13
Colin P De Souza, Shahr B Hashmi, Aysha H Osmani, Peter Andrews, Carol S Ringelberg, Jay C Dunlap, Stephen A Osmani
The filamentous fungi are an ecologically important group of organisms which also have important industrial applications but devastating effects as pathogens and agents of food spoilage. Protein kinases have been implicated in the regulation of virtually all biological processes but how they regulate filamentous fungal specific processes is not understood. The filamentous fungus Aspergillus nidulans has long been utilized as a powerful molecular genetic system and recent technical advances have made systematic approaches to study large gene sets possible...
2013: PloS One
https://www.readbyqxmd.com/read/22854045/the-conserved-kinase-srpk-regulates-karyosome-formation-and-spindle-microtubule-assembly-in-drosophila-oocytes
#14
Benjamin J Loh, C Fiona Cullen, Nina Vogt, Hiroyuki Ohkura
In Drosophila oocytes, after the completion of recombination, meiotic chromosomes form a compact cluster called the karyosome within the nucleus, and later assemble spindle microtubules without centrosomes. Although these oocyte-specific phenomena are also observed in humans, their molecular basis is not well understood. Here, we report essential roles for the conserved kinase SRPK in both karyosome formation and spindle microtubule assembly in oocytes. We have identified a female-sterile srpk mutant through a cytological screen for karyosome defects...
October 1, 2012: Journal of Cell Science
https://www.readbyqxmd.com/read/22727668/the-akt-srpk-sr-axis-constitutes-a-major-pathway-in-transducing-egf-signaling-to-regulate-alternative-splicing-in-the-nucleus
#15
Zhihong Zhou, Jinsong Qiu, Wen Liu, Yu Zhou, Ryan M Plocinik, Hairi Li, Qidong Hu, Gourisanker Ghosh, Joseph A Adams, Michael G Rosenfeld, Xiang-Dong Fu
Pre-mRNA splicing is regulated by developmental and environmental cues, but little is known about how specific signals are transduced in mammalian cells to regulate this critical gene expression step. Here, we report massive reprogramming of alternative splicing in response to EGF signaling. By blocking individual branches in EGF signaling, we found that Akt activation plays a major role, while other branches, such as the JAK/STAT and ERK pathways, make minor contributions to EGF-induced splicing. Activated Akt next branches to SR protein-specific kinases, rather than mTOR, by inducing SRPK autophosphorylation that switches the splicing kinases from Hsp70- to Hsp90-containing complexes...
August 10, 2012: Molecular Cell
https://www.readbyqxmd.com/read/22683458/interacting-factors-and-cellular-localization-of-sr-protein-specific-kinase-dsk1
#16
Zhaohua Tang, Maria Luca, Laura Taggart-Murphy, Jessica Portillio, Cathey Chang, Ayse Guven, Ren-Jang Lin, Johanne Murray, Antony Carr
Schizosaccharomyces pombe Dsk1 is an SR protein-specific kinase (SRPK), whose homologs have been identified in every eukaryotic organism examined. Although discovered as a mitotic regulator with protein kinase activity toward SR splicing factors, it remains largely unknown about what and how Dsk1 contributes to cell cycle and pre-mRNA splicing. In this study, we investigated the Dsk1 function by determining interacting factors and cellular localization of the kinase. Consistent with its reported functions, we found that pre-mRNA processing and cell cycle factors are prominent among the proteins co-purified with Dsk1...
October 1, 2012: Experimental Cell Research
https://www.readbyqxmd.com/read/22137642/regulation-and-differential-role-of-the-tissue-factor-isoforms-in-cardiovascular-biology
#17
REVIEW
Andreas Eisenreich, Ursula Rauch
Alternative pre-mRNA splicing is an essential mechanism regulating protein diversity and functional plasticity of the proteome in response to environmental changes. Several factors are involved in this regulatory mechanism, such as serine/arginine-rich (SR) proteins, the Cdc2-like kinase (Clk) family, the dual-specificity tyrosine phosphorylation regulated kinases, the SR protein kinases (SRPK) 1 and 2, the protein kinase B (PKB,Akt), and the DNA topoisomerase I (DNA topo I). Dynamic changes of the phosphorylation state of SR proteins, mediated by the kinases mentioned previously, play an important role in alternative splicing regulation...
August 2010: Trends in Cardiovascular Medicine
https://www.readbyqxmd.com/read/22114561/a-kinase-chaperones-hepatitis-b-virus-capsid-assembly-and-captures-capsid-dynamics-in-vitro
#18
Chao Chen, Joseph Che-Yen Wang, Adam Zlotnick
The C-terminal domain (CTD) of Hepatitis B virus (HBV) core protein is involved in regulating multiple stages of the HBV lifecycle. CTD phosphorylation correlates with pregenomic-RNA encapsidation during capsid assembly, reverse transcription, and viral transport, although the mechanisms remain unknown. In vitro, purified HBV core protein (Cp183) binds any RNA and assembles aggressively, independent of phosphorylation, to form empty and RNA-filled capsids. We hypothesize that there must be a chaperone that binds the CTD to prevent self-assembly and nonspecific RNA packaging...
November 2011: PLoS Pathogens
https://www.readbyqxmd.com/read/21600902/regulating-sr-protein-phosphorylation-through-regions-outside-the-kinase-domain-of-srpk1
#19
Ryan M Plocinik, Sheng Li, Tong Liu, Kendra L Hailey, Jennifer Whitesides, Jennifer Whitehouse, Chen-Ting Ma, Xiang-Dong Fu, Gourisankar Gosh, Virgil L Woods, Patricia A Jennings, Joseph A Adams
SR proteins (splicing factors containing arginine-serine repeats) are essential splicing factors whose phosphorylation by the SR-specific protein kinase (SRPK) family regulates nuclear localization and mRNA processing activity. In addition to an N-terminal extension with unknown function, SRPKs contain a large, nonhomologous spacer insert domain (SID) that bifurcates the kinase domain and anchors the kinase in the cytoplasm through interactions with chaperones. While structures for the kinase domain are now available, constructs that include regions outside this domain have been resistant to crystallographic elucidation...
July 1, 2011: Journal of Molecular Biology
https://www.readbyqxmd.com/read/21555914/srsf2-is-required-for-sodium-butyrate-mediated-p21-waf1-induction-and-premature-senescence-in-human-lung-carcinoma-cell-lines
#20
Valerie Edmond, Christian Brambilla, Elisabeth Brambilla, Sylvie Gazzeri, Beatrice Eymin
Sodium butyrate (NaBu) is a histone deacetylase inhibitor that exhibits numerous antiproliferative activities in various cancer cell lines, notably through the accumulation of the well-known cyclin-dependent kinase inhibitor p21(WAF1) . SRSF2 belongs to the family of SR proteins that are crucial regulators of constitutive and alternative pre-mRNA splicing. Previous studies demonstrated that NaBu alters pre-mRNA splicing patterns through upregulation of SR proteins expression in non-tumor cells. In this study, we show that NaBu also induces the accumulation of SRSF2 in human lung carcinoma cell lines...
June 15, 2011: Cell Cycle
keyword
keyword
82790
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"