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https://www.readbyqxmd.com/read/29149086/physiological-peculiarities-of-lignin-modifying-enzyme-production-by-the-white-rot-basidiomycete-coriolopsis-gallica-strain-bcc-142
#1
Vladimir Elisashvili, Eva Kachlishvili, Mikheil D Asatiani, Ramona Darlington, Katarzyna H Kucharzyk
Sixteen white-rot Basidiomycota isolates were screened for production of lignin-modifying enzymes (LME) in glycerol- and mandarin peel-containing media. In the synthetic medium, Cerrena unicolor strains were the only high laccase (Lac) (3.2-9.4 U/mL) and manganese peroxidase (MnP) (0.56-1.64 U/mL) producers while one isolate Coriolopsis gallica was the only lignin peroxidase (LiP) (0.07 U/mL) producer. Addition of mandarin peels to the synthetic medium promoted Lac production either due to an increase in fungal biomass (Funalia trogii, Trametes hirsuta, and T...
November 17, 2017: Microorganisms
https://www.readbyqxmd.com/read/29148030/incidence-and-quantitative-analysis-of-dissociated-optic-nerve-fiber-layer-appearance-real-loss-of-retinal-nerve-fiber-layer
#2
Yoon J Kim, Kyoung S Lee, Soo G Joe, June-Gone Kim
PURPOSE: To assess the association between appearance of dissociated optic nerve fiber layer (DONFL) after internal limiting membrane (ILM) peeling and changes in the macular nerve fiber layer (NFL) area by spectral-domain optical coherence tomography (SD-OCT). METHODS: This study included 132 consecutive patients who had successfully undergone vitrectomy with ILM peeling for idiopathic epiretinal membrane or macular holes and had been followed up for ≥6 months...
November 4, 2017: European Journal of Ophthalmology
https://www.readbyqxmd.com/read/29146916/deposition-of-topological-silicene-germanene-and-stanene-on-graphene-covered-sic-substrates
#3
Filipe Matusalem, Daniel S Koda, Friedhelm Bechstedt, Marcelo Marques, Lara K Teles
Growth of X-enes, such as silicene, germanene and stanene, requires passivated substrates to ensure the survival of their exotic properties. Using first-principles methods, we study as-grown graphene on polar SiC surfaces as suitable substrates. Trilayer combinations with coincidence lattices with large hexagonal unit cells allow for strain-free group-IV monolayers. In contrast to the Si-terminated SiC surface, van der Waals-bonded honeycomb X-ene/graphene bilayers on top of the C-terminated SiC substrate are stable...
November 16, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29146338/analysis-of-changes-in-volatile-constituents-and-expression-of-genes-involved-in-terpenoid-metabolism-in-oleocellosis-peel
#4
Jiao Xie, Lili Deng, Yahan Zhou, Shixiang Yao, Kaifang Zeng
Oleocellosis is a serious physiological disorder in citrus fruit that mainly results in appearance and quality deterioration. It has been well established that the occurrence of oleocellosis is highly correlated with the release of peel oil from citrus fruit, while there is little information on the dynamic changes in the content of the volatile constituents and the expression of genes involved in terpenoid metabolism during oleocellosis development. In the present research, large changes in the volatile profiles and gene expression in terpenoid metabolism were observed in oleocellosis peels compared to healthy ones...
March 15, 2018: Food Chemistry
https://www.readbyqxmd.com/read/29144280/selective-ion-permeation-through-strained-and-charged-graphene-membranes
#5
Kun Li, Yi Tao, Zhongwu Li, Jingjie Sha, Yunfei Chen
By means of molecular dynamics (MD) simulations and density functional theory (DFT) calculations, we demonstrate that stretched and charged graphene can act as ion sieve membranes. It is observed that loading 30% strain on graphene can induce pores in the dense electron cloud to allow ions to pass through the aromatic rings. Meanwhile, charged surface is helpful to peel the hydration layers from the ions and decrease the energy barrier for ion translocation through nanopores. Our results suggest that with membrane charge density of 6...
November 16, 2017: Nanotechnology
https://www.readbyqxmd.com/read/29143676/the-future-of-viral-hepatitis-testing-innovations-in-testing-technologies-and-approaches
#6
REVIEW
Rosanna W Peeling, Debrah I Boeras, Francesco Marinucci, Philippa Easterbrook
A large burden of undiagnosed hepatitis virus cases remains globally. Despite the 257 million people living with chronic hepatitis B virus infection, and 71 million with chronic viraemic HCV infection, most people with hepatitis remain unaware of their infection. Advances in rapid detection technology have created new opportunities for enhancing access to testing and care, as well as monitoring of treatment. This article examines a range of other technological innovations that can be leveraged to provide more affordable and simplified approaches to testing for HBV and HCV infection and monitoring of treatment response...
November 1, 2017: BMC Infectious Diseases
https://www.readbyqxmd.com/read/29143673/the-heptestcontest-a-global-innovation-contest-to-identify-approaches-to-hepatitis-b-and-c-testing
#7
Joseph D Tucker, Kathrine Meyers, John Best, Karyn Kaplan, Razia Pendse, Kevin A Fenton, Isabelle Andrieux-Meyer, Carmen Figueroa, Pedro Goicochea, Charles Gore, Azumi Ishizaki, Giten Khwairakpam, Veronica Miller, Antons Mozalevskis, Michael Ninburg, Ponsiano Ocama, Rosanna Peeling, Nick Walsh, Massimo G Colombo, Philippa Easterbrook
BACKGROUND: Innovation contests are a novel approach to elicit good ideas and innovative practices in various areas of public health. There remains limited published literature on approaches to deliver hepatitis testing. The purpose of this innovation contest was to identify examples of different hepatitis B and C approaches to support countries in their scale-up of hepatitis testing and to supplement development of formal recommendations on service delivery in the 2017 World Health Organization hepatitis B and C testing guidelines...
November 1, 2017: BMC Infectious Diseases
https://www.readbyqxmd.com/read/29143619/diagnostic-accuracy-of-tests-to-detect-hepatitis-b-surface-antigen-a-systematic-review-of-the-literature-and-meta-analysis
#8
Ali Amini, Olivia Varsaneux, Helen Kelly, Weiming Tang, Wen Chen, Debrah I Boeras, Jane Falconer, Joseph D Tucker, Roger Chou, Azumi Ishizaki, Philippa Easterbrook, Rosanna W Peeling
BACKGROUND: Chronic Hepatitis B Virus (HBV) infection is characterised by the persistence of hepatitis B surface antigen (HBsAg). Expanding HBV diagnosis and treatment programmes into low resource settings will require high quality but inexpensive rapid diagnostic tests (RDTs) in addition to laboratory-based enzyme immunoassays (EIAs) to detect HBsAg. The purpose of this review is to assess the clinical accuracy of available diagnostic tests to detect HBsAg to inform recommendations on testing strategies in 2017 WHO hepatitis testing guidelines...
November 1, 2017: BMC Infectious Diseases
https://www.readbyqxmd.com/read/29143615/diagnostic-accuracy-of-tests-to-detect-hepatitis-c-antibody-a-meta-analysis-and-review-of-the-literature
#9
Weiming Tang, Wen Chen, Ali Amini, Debi Boeras, Jane Falconer, Helen Kelly, Rosanna Peeling, Olivia Varsaneux, Joseph D Tucker, Philippa Easterbrook
BACKGROUND: Although direct-acting antivirals can achieve sustained virological response rates greater than 90% in Hepatitis C Virus (HCV) infected persons, at present the majority of HCV-infected individuals remain undiagnosed and therefore untreated. While there are a wide range of HCV serological tests available, there is a lack of formal assessment of their diagnostic performance. We undertook a systematic review and meta-analysis to evaluate he diagnostic accuracy of available rapid diagnostic tests (RDT) and laboratory based EIA assays in detecting antibodies to HCV...
November 1, 2017: BMC Infectious Diseases
https://www.readbyqxmd.com/read/29142559/visual-and-anatomical-outcome-of-macular-hole-surgery-at-a-tertiary-healthcare-facility
#10
Komalta Kumari, Muhammad Ali Tahir, Alyscia Cheema
Objective: To assess visual and anatomical outcome of full thickness macular hole (FTMH) surgery with ILM peeling using brilliant blue G dye. Methods: Thirty patients who had clinically evident macular hole were selected. Pre-operative Optical Coherence Tomography (OCT) was done. In all cases vitrectomy was performed via 23guage 3 ports pars plana (3PPV) vitrectomy system and Brilliant blue G dye, 0.5ml dye was injected over macula which resulted in light blue stain of ILM and peeling was performed around hole in circular motion and after gas fluid exchange gas tamponade with SF6 was done...
September 2017: Pakistan Journal of Medical Sciences Quarterly
https://www.readbyqxmd.com/read/29142390/a-comprehensive-study-on-fast-dispersible-and-slow-releasing-characteristic-of-orange-peel-pectin-in-relation-to-established-synthetic-polymer
#11
Pranati Srivastava, Mahendra Singh, Shilpi Bhargava
Introduction: In the present work, the method to extract, isolate, and characterize orange peel pectin using soxhlation, and thereafter, the use of this polymer-polymer in the formulation of fast dispersable and slow-releasing tablet has been studied. Thereafter, the evaluation and comparison of fast dispersible/slow-releasing tablets using orange peel pectin versus prepared using sodium starch glycolate (SSG) were carried out. Materials and Methods: In the present investigation, extraction methodology was employed for isolation of pectin from orange peels...
October 2017: Pharmacognosy Magazine
https://www.readbyqxmd.com/read/29142163/strain-improvement-studies-on-microbacterium-foliorum-ga2-for-production-of-%C3%AE-amylase-in-solid-state-fermentation-biochemical-characteristics-and-wash-performance-analysis-at-low-temperatures
#12
Roohi, Mohammed Kuddus
Microbacterium foliorum GA2, an alkali-tolerant bacterium, was randomly mutated using UV radiation and sodium azide to obtain a mutant with a higher cold-active extracellular amylolytic activity. A mutant, designated as MFSD20, was selected owing to its higher amylase activity at 20°C. Under optimized conditions, amylase production was achieved best with raw banana peels (5000 units) in solid-state fermentation (SSF). The enzyme was purified by salt precipitation and chromatographic methods and afterwards characterized biochemically...
November 15, 2017: Journal of General and Applied Microbiology
https://www.readbyqxmd.com/read/29137742/ultrasound-coupled-with-supercritical-carbon-dioxide-for-exfoliation-of-graphene-simulation-and-experiment
#13
Yanzhe Gai, Wucong Wang, Ding Xiao, Yaping Zhao
Ultrasound coupled with supercritical CO2 has become an important method for exfoliation of graphene, but behind which a peeling mechanism is unclear. In this work, CFD simulation and experiment were both investigated to elucidate the mechanism and the effects of the process parameters on the exfoliation yield. The experiments and the CFD simulation were conducted under pressure ranging from 8MPa to 16MPa, the ultrasonic power ranging from 12W to 240W and the frequency of 20kHz. The numerical analysis of fluid flow patterns and pressure distributions revealed that the fluid shear stress and the periodical pressure fluctuation generated by ultrasound were primary factors in exfoliating graphene...
March 2018: Ultrasonics Sonochemistry
https://www.readbyqxmd.com/read/29137576/perioperative-assessment-of-older-surgical-patients-using-a-frailty-index-feasibility-and-association-with-adverse-post-operative-outcomes
#14
H Lin, N M Peel, I A Scott, D L Vardesh, P Sivalingam, R L McBride, J J Morong, M J Nelson, R E Hubbard
This study aimed to examine the feasibility of using a frailty index (FI) based on comprehensive geriatric assessment (CGA), to assess the level of frailty in older surgical patients preoperatively and to evaluate the association of FI-CGA with poorer postoperative outcomes. Two hundred and forty-six patients aged ≥70 years undergoing intermediate- to high-risk surgery in a tertiary hospital were recruited. Frailty was assessed using a 57-item FI-CGA form, with fit, intermediate frail, and frail patients defined as FI ≤0...
November 2017: Anaesthesia and Intensive Care
https://www.readbyqxmd.com/read/29135801/timing-and-outcome-of-surgery-for-persistent-macular-hole
#15
Rutul Patel, Mahesh Gopalakrishnan, Anantharaman Giridhar
PURPOSE: To evaluate the efficacy and optimum timing for resurgery in case of persistent macular hole (MH). METHODS: Twenty-five eyes of 25 patients who underwent fluid-air exchange with intravitreal C3F8 for persistent MH after vitrectomy + internal limiting membrane peeling + SF6 were included in the study. Best-corrected visual acuity, optical coherence tomography features including base diameter and configuration of MH, and time duration between the two surgeries were noted...
November 10, 2017: Retina
https://www.readbyqxmd.com/read/29134675/analytical-approach-dissipation-pattern-and-risk-assessment-of-pesticide-residue-in-green-leafy-vegetables-a-comprehensive-review
#16
REVIEW
Waziha Farha, A M Abd El-Aty, Md Musfiqur Rahman, Ji Hoon Jeong, Ho-Chul Shin, Jing Wang, Sung Shik Shin, Jae-Han Shim
The category of "leafy vegetables" comprises a wide range of plants, including cabbage, lettuce, leeks, spinach, Swiss chard, and kale, and it forms a significant component of the human diet. Typically, leafy vegetables are low in calories and fat, are great sources of vitamins, protein, dietary fibre, minerals (including iron, calcium, and nitrates), and are rich in phytochemicals. To counter the impact of pests on vegetables, a broad variety of pesticides is used. Because of their large surface areas, leafy vegetables are expected to have high residual pesticide levels...
November 14, 2017: Biomedical Chromatography: BMC
https://www.readbyqxmd.com/read/29134123/green-synthesis-of-capped-gold-nanoparticles-and-their-effect-on-gram-positive-and-gram-negative-bacteria
#17
Yashvant Rao, Gajendra K Inwati, Man Singh
Aim: We report synthesis of capped gold nanoparticles (C-AuNPs) of ≈20-30 nm by reducing HAuCl4 with flower and leaf extracts of Ocimum tenuiflorum, leaves of Azadirachta indica and Mentha spicata and peel of Citrus sinensis plants. Methods: Atomic force microscopy (AFM) and transmission electron microscopy (TEM) determined their size, shape and topographical structures. The C-AuNPs with UV-Vis spectrophotometer produced a maximum absorption within 530-535 nm wavelengths...
November 2017: Future Science OA
https://www.readbyqxmd.com/read/29133668/vitrectomy-for-full-thickness-macular-hole-in-adult-onset-coats-disease
#18
Vinod Kumar, Pradeep Kumar, Gaurav Garg, Saurabh Damodaran
The occurrence of full thickness macular hole in Coats' disease is extremely rare. The purpose of this case report is to report pars plana vitrectomy for the treatment of full thickness macular hole in a patient with adult onset Coats disease. A young male presented with decreased vision in his right eye because of full thickness macular hole. The macular hole was found to be associated with adult onset Coats' disease that was evident on ultra-wide field imaging. The patient underwent laser photocoagulation to the vascular telangiectasia followed by pars plana vitrectomy, large internal limiting membrane peeling and gas tamponade...
November 2017: Indian Journal of Ophthalmology
https://www.readbyqxmd.com/read/29130985/heptamethoxyflavone-reduces-phosphodiesterase-activity-and-t-cell-growth-in-vitro
#19
Yui Hamada, Mitsunari Nakajima, Kazuna Tsuzuki, Yoshiaki Amakura, Morio Yoshimura, Satoshi Okuyama, Yoshiko Furukawa
In a previous study, we reported that interleukin-4 production was reduced in spleen cells of mice administered 3,5,6,7,8,3',4'-heptamethoxyflavone (HMF), which is a polymethoxyflavone found at high concentrations in the peel of various citrus fruits. In this study, we investigated the function of HMF on the growth of T cells cultured from the spleens of mice. HMF decreased the reduction of 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) by anti-CD3/CD28 antibody-stimulated mouse spleen cells...
November 8, 2017: International Archives of Allergy and Immunology
https://www.readbyqxmd.com/read/29130237/in-situ-forming-oxidised-hyaluronic-acid-adipic-acid-dihydrazide-hydrogel-for-prevention-of-epidural-fibrosis-after-laminectomy
#20
M H Hu, K C Yang, Y H Sun, Y C Chen, S H Yang, F H Lin
Post-operative epidural fibrosis is a biological response after laminectomy that may lead to clinical symptoms, such as radicular pain. An ideal material for prevention of epidural fibrosis should be able to inhibit fibroblast adhesions and reduce formation of scar tissue. An injectable hydrogel would be the material of choice for this purpose, since it could fill an irregular surgical defect completely, gelate in situ and be delivered in a minimally-invasive manner. The objective of this study was to evaluate, in vitro and in vivo, the cytocompatibility and anti-adhesive effect of an oxidised hyaluronic acid/adipic acid dihydrazide (oxi-HA/ADH) hydrogel...
November 13, 2017: European Cells & Materials
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