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dsRNA, virus, J2

Haroun Zangger, Catherine Ronet, Chantal Desponds, F Matthew Kuhlmann, John Robinson, Mary-Anne Hartley, Florence Prevel, Patrik Castiglioni, Francine Pratlong, Patrick Bastien, Norbert Müller, Laurent Parmentier, Nancy Gore Saravia, Stephen M Beverley, Nicolas Fasel
BACKGROUND: Patients suffering from cutaneous leishmaniasis (CL) caused by New World Leishmania (Viannia) species are at high risk of developing mucosal (ML) or disseminated cutaneous leishmaniasis (DCL). After the formation of a primary skin lesion at the site of the bite by a Leishmania-infected sand fly, the infection can disseminate to form secondary lesions. This metastatic phenotype causes significant morbidity and is often associated with a hyper-inflammatory immune response leading to the destruction of nasopharyngeal tissues in ML, and appearance of nodules or numerous ulcerated skin lesions in DCL...
2013: PLoS Neglected Tropical Diseases
Sarah J Richardson, Abby Willcox, David A Hilton, Sisko Tauriainen, Heikki Hyoty, Adrian J Bone, Alan K Foulis, Noel G Morgan
BACKGROUND: The detection of viral infection in paraffin-embedded, formalin-fixed tissue is notoriously difficult and often requires inherent knowledge about the specific virus being sought. For this reason, there is an ongoing need for reagents and methods which can identify a range of different virus types in paraffin embedded tissue. OBJECTIVES: The aim of this study was to optimise and validate the use of antisera directed against dsRNA (>50 bp in length) in paraffin-embedded formalin-fixed tissue samples...
November 2010: Journal of Clinical Virology: the Official Publication of the Pan American Society for Clinical Virology
B Morgun, A Richter, S D Deshmukh, V Stepanyuk, Katalin Kálai, G Nagy, L Hufnagel, Noémi Lukács
Expression of antibodies or antibody fragments in plants is a useful tool for producing active antibody derivatives for diagnostic or pharmaceutical purposes as well as for immunomodulation. We investigated the effect of cellular expression site on the stability and yield of double-stranded RNA (dsRNA)-specific single-chain Fv-fragments (scFv) in transgenic tobacco. Two antibodies (J2 and P6) belonging to the V23(J558) heavy chain variable gene family but differing in the light chain variable domain were used...
June 2006: Acta Biologica Hungarica
B V Morgun, S Deshmuk, V Stepaniuk, T Pasternak, N Lukach
Antibody expression and immunomodulation are modern molecular techniques to produce pharmaceuticals and to interfere with cellular metabolism or pathogen infectivity in plants. Nonetheless, there is still no generally applicable strategy to express correctly folded active antibodies or antibody fragments in different cell compartments. To facilitate expression, single-chain antibody fragments (scFvs) were made of mouse monoclonal antibodies, J2 and P6 that specifically recognize double-stranded RNA (dsRNA)...
May 2005: T︠S︡itologii︠a︡ i Genetika
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