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Silio Lima Moura, Laura Martinez Fajardo, Leonardo Dos Anjos Cunha, Maria Del Pilar Taboada Sotomayor, Francisco Bolivar Correto Machado, Luiz Fernando Araújo Ferrão, Maria Isabel Pividori
This study addresses the rational design of a magnetic molecularly imprinted polymer (magnetic-MIP) for the selective recognition of the hormone levothyroxine. The theoretical study was carried out by the density functional theory (DFT) computations considering dispersion interaction energies, and using the D2 Grimme's correction. The B97-D/def2-SV(P)/PCM method is used not only for studying the structure of the template the and monomer-monomer interactions, but also to assess the stoichiometry, noncovalent binding energies, solvation effects and thermodynamics properties such as binding energy...
January 31, 2018: Biosensors & Bioelectronics
Manuel Gregoritza, Kathrin Abstiens, Moritz Graf, Achim M Goepferich
Reducing burst effects, providing controlled release, and safeguarding biologics against degradation are a few of several highly attractive applications for microgels in the field of controlled release. However, the incorporation of proteins into microgels without impairing stability is highly challenging. In this proof of concept study, the combination of microfluidics and thiol-ene photoclick chemistry was evaluated for the fabrication of antibody-loaded microgels with narrow size distribution. Norbornene-modified eight-armed poly(ethylene glycol) with an average molecular mass of 10,000 Da, 20,000 Da, or 40,000 Da were prepared as macromonomers for microgel formation...
February 19, 2018: European Journal of Pharmaceutics and Biopharmaceutics
F Kashanian, M M Masoudi, A Shamloo, M Habibi-Rezaei, A A Moosavi-Movahedi
Miniaturized systems based on the principles of microfluidics are widely used in various fields, such as biochemical and biomedical applications. Systematic design processes are demanded the proper use of these microfluidic devices based on mathematical simulations. Aggregated proteins (e.g., inclusion bodies) in solution with chaotropic agents (such as urea) at high concentration in combination with reducing agents are denatured. Refolding methods to achieve the native proteins from inclusion bodies of recombinant protein relying on denaturant dilution or dialysis approaches for suppressing protein aggregation is very important in the industrial field...
February 22, 2018: Bioprocess and Biosystems Engineering
Jimmy Ly, Noel S Ha, Shilin Cheung, R Michael van Dam
Miniaturized synthesis of positron emission tomography (PET) tracers is poised to offer numerous advantages including reduced tracer production costs and increased availability of diverse tracers. While many steps of the tracer production process have been miniaturized, there has been relatively little development of microscale systems for the quality control (QC) testing process that is required by regulatory agencies to ensure purity, identity, and biological safety of the radiotracer before use in human subjects...
February 22, 2018: Analytical and Bioanalytical Chemistry
Lalit Bansal, Apratim Sanyal, Prasenjit Kabi, Binita Pathak, Saptarshi Basu
Evaporating sessile functional droplets act as the fundamental building block that control the cumulative outcome of many industrial and biological applications such as surface patterning, 3D printing, photonic crystals, DNA sequencing, to name a few. Additionally, a drying single sessile droplet forms a high throughput processing technique using low material volume which is especially suitable for medical diagnosis. Sessile droplet also provides an elementary platform to study and analyze fundamental interfacial processes at various lengthscale ranging from macroscopically observable wetting and evaporation to microfluidic transport to interparticle forces operating at a nanometric lengthscale...
February 22, 2018: Langmuir: the ACS Journal of Surfaces and Colloids
Casey W Quinn, David Cate, Daniel Miller-Lionberg, Thomas Reilly, John Volckens, Charles S Henry
Metal contamination of natural and drinking water systems poses hazards to public and environmental health. Quantifying metal concentrations in water typically requires sample collection in the field followed by expensive laboratory analysis that can take days to weeks to obtain results. The objective of this work was to develop a low-cost, field-deployable method to quantify trace levels of copper in drinking water by coupling solid-phase extraction/preconcentration with a microfluidic paper-based analytical device...
February 22, 2018: Environmental Science & Technology
Shang Sun, Wenhong Yang, Chen Zhang, Jixiang Jing, Yisheng Gao, Xiaoyi Yu, Qinghai Song, Shumin Xiao
Structural colors arising from all-dielectric nanostructures are very promising for high-resolution color nanoprinting and high-density optical storage. However, once the all-dielectric nanostructures are fabricated, their optical performances are usually static or change slowly, significantly limiting the practical applications in advanced displays. Herein, we experimentally demonstrate the real-time tunable colors with microfluidic reconfigurable all-dielectric metasurfaces. The metasurface is composed of an array of TiO2 nanoblocks, which are embedded in a polymeric microfluidic channel...
February 22, 2018: ACS Nano
Jeremy M Schieferstein, Ashtamurthy S Pawate, Michael J Varel, Sudipto Guha, Ieva Astrauskaite, Robert B Gennis, Paul J A Kenis
Crystallization of membrane proteins is a critical step for uncovering atomic resolution 3-D structures and elucidating structure-function relationships. Microseeding, the process of transferring sub-microscopic crystal nuclei from initial screens into new crystallization experiments, is an effective, yet underutilized approach to grow crystals suitable for X-ray crystallography. Here, we report simplified methods for crystallization of membrane proteins that utilize microseeding in X-ray transparent microfluidic chips...
February 22, 2018: Lab on a Chip
John P Goertz, Ian M White
Medical diagnostics and basic research in low-resource settings require automated reactions controlled in a simple, portable manner. Here, we present a novel platform that enables simple automation of multi-step reactions to facilitate robust, hands-free assay operation without complex microfluidics or paperfluidics. We separate reagent zones in a conventional PCR tube via solid layers of purified higher alkanes. Reagents can be mixed on demand by simply raising the temperature above the melting point of the alkane partition that separates the two zones...
February 22, 2018: Analytical Chemistry
Stephanie R Harrison, Thomas Scambler, Lylia Oubussad, Chi Wong, Miriam Wittmann, Michael F McDermott, Sinisa Savic
Tumor necrosis factor (TNF)-receptor-associated periodic fever syndrome (TRAPS) is a rare monogenic autoinflammatory disorder characterized by mutations in the TNFRSF1A gene, causing TNF-receptor 1 (TNFR1) misfolding, increased cellular stress, activation of the unfolded protein response (UPR), and hyperresponsiveness to lipopolysaccharide (LPS). Both microRNA (miR)-146a and miR-155 provide negative feedback for LPS-toll-like receptor 2/4 signaling and cytokine production, through regulation of nuclear factor kappa B (NF-κB)...
2018: Frontiers in Immunology
Jared L Wilmoth, Peter W Doak, Andrea Timm, Michelle Halsted, John D Anderson, Marta Ginovart, Clara Prats, Xavier Portell, Scott T Retterer, Miguel Fuentes-Cabrera
The factors leading to changes in the organization of microbial assemblages at fine spatial scales are not well characterized or understood. However, they are expected to guide the succession of community development and function toward specific outcomes that could impact human health and the environment. In this study, we put forward a combined experimental and agent-based modeling framework and use it to interpret unique spatial organization patterns of H1-Type VI secretion system (T6SS) mutants of P . aeruginosa under spatial confinement...
2018: Frontiers in Microbiology
Xu Hou, Jianyu Li, Alexander B Tesler, Yuxing Yao, Miao Wang, Lingli Min, Zhizhi Sheng, Joanna Aizenberg
Microscale flows of fluids are mainly guided either by solid matrices or by liquid-liquid interfaces. However, the solid matrices are plagued with persistent fouling problems, while liquid-liquid interfaces are limited to low-pressure applications. Here we report a dynamic liquid/solid/gas material containing both air and liquid pockets, which are formed by partially infiltrating a porous matrix with a functional liquid. Using detailed theoretical and experimental data, we show that the distribution of the air- and liquid-filled pores is responsive to pressure and enables the formation and instantaneous recovery of stable liquid-liquid interfaces that sustain a wide range of pressures and prevent channel contamination...
February 21, 2018: Nature Communications
Nachiket Shembekar, Hongxing Hu, David Eustace, Christoph A Merten
Monoclonal antibodies are a main player in modern drug discovery. Many antibody screening formats exist, each with specific advantages and limitations. Nonetheless, it remains challenging to screen antibodies for the binding of cell-surface receptors (the most important class of all drug targets) or for the binding to target cells rather than purified proteins. Here, we present a high-throughput droplet microfluidics approach employing dual-color normalized fluorescence readout to detect antibody binding. This enables us to obtain quantitative data on target cell recognition, using as little as 33 fg of IgG per assay...
February 20, 2018: Cell Reports
Mathias Girault, Thomas Beneyton, Deniz Pekin, Lionel Buisson, Sabrina Bichon, Celine Charbonnier, Yolanda Del Amo, Jean Christophe Baret
One way for phytoplankton to survive to orthophosphate depletion is to utilize the dissolved organic phosphorus by expressing alkaline phosphatase. The actual methods to assay the alkaline phosphate activity - either in bulk or as a presence-absence of enzyme activity - fail to provide information on individual living cells. In this context, we develop a new microfluidic method to compartmentalize cells in 0.5 nanoliter water-in-oil droplets and measure the alkaline phosphatase activity at the single cell level...
February 21, 2018: Analytical Chemistry
Chan Ho Park, Sangmin Lee, Ghasidit Pornnoppadol, Yoon Sung Nam, Shin-Hyun Kim, Bumjoon J Kim
We report the design of a novel microcapsule platform for in-situ pH sensing and photothermal heating, involving the encapsulation of pH-responsive polymer-coated MoS2 nanosheets (NSs) in microcapsules with an aqueous core and a semipermeable polymeric shell. The MoS2 NSs were functionalized with pH-responsive polymers having fluorescent groups at the distal end to provide pH-sensitive Förster resonance energy transfer (FRET) effect. The pH-responsive polymers were carefully designed to produce a dramatic change in polymer conformation, which translated to a change in FRET efficiency near pH 7...
February 21, 2018: ACS Applied Materials & Interfaces
Changqing Yin, Yuhui Wang, Jia Ji, Bo Cai, Hao Chen, Zhonghua Yang, Kun Wang, Changliang Luo, Wu-Wen Zhang, Chunhui Yuan, Fubing Wang
Here, we constructed a novel dual-antibody-functionalized microfluidic device by employing antibodies against PSMA and EpCAM to capture CTCs from PCa patients in order to capture multiple subpopulations of CTCs at different metastatic stages. In vitro experiments with dual capture system for capturing both LnCAP and LnCAP-EMT cells have shown significantly enhanced capture efficiency as compared to that of EpCAM single capture system. Furthermore, dual capture system could successfully identify CTCs in 20 out of 24 (83...
February 21, 2018: Analytical Chemistry
A Dawson, C Dyer, J Macfie, J Davies, L Karsai, J Greenman, M Jacobsen
[This corrects the article DOI: 10.1063/1.4964813.].
January 2018: Biomicrofluidics
Amir Reza Peimani, Georg Zoidl, Pouya Rezai
The zebrafish is a lower vertebrate model organism offering multiple applications for both fundamental and biomedical research into the nervous system from genes to behaviour. Investigation of zebrafish larvae's movement in response to various stimuli, which involves the dopaminergic system, is of interest in the field of sensory-motor integration. Nevertheless, the conventional methods of movement screening in Petri dishes and multi-well plates are mostly qualitative, uncontrollable, and inaccurate in terms of stimulus delivery and response analysis...
January 2018: Biomicrofluidics
Hamed Haddadi, Hamed Naghsh-Nilchi, Dino Di Carlo
Label-free separation of viable cancer cells using vortical microfluidic flows has been introduced as a feasible cell collection method in oncological studies. Besides the clinical importance, the physics of particle interactions with the vortex that forms in a wall-confined geometry of a microchannel is a relatively new area of fluid dynamics. In our previous work [Haddadi and Di Carlo, J. Fluid. Mech. 811 , 436-467 (2017)], we have introduced distinct aspects of inertial flow of dilute suspensions over cavities in a microchannel such as breakdown of the separatrix and formation of stable limit cycle orbits for finite size polystyrene particles...
January 2018: Biomicrofluidics
Samar Damiati, Uday B Kompella, Safa A Damiati, Rimantas Kodzius
Microfluidic devices present unique advantages for the development of efficient drug carrier particles, cell-free protein synthesis systems, and rapid techniques for direct drug screening. Compared to bulk methods, by efficiently controlling the geometries of the fabricated chip and the flow rates of multiphase fluids, microfluidic technology enables the generation of highly stable, uniform, monodispersed particles with higher encapsulation efficiency. Since the existing preclinical models are inefficient drug screens for predicting clinical outcomes, microfluidic platforms might offer a more rapid and cost-effective alternative...
February 16, 2018: Genes
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