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https://www.readbyqxmd.com/read/28448048/time-resolved-electrospray-ionization-hydrogen-deuterium-exchange-mass-spectrometry-for-studying-protein-structure-and-dynamics
#1
Cristina Lento, Shaolong Zhu, Kerene A Brown, Ruth Knox, Peter Liuni, Derek J Wilson
Intrinsically disordered proteins (IDPs) have long been a challenge to structural biologists due to their lack of stable secondary structure elements. Hydrogen-Deuterium Exchange (HDX) measured at rapid time scales is uniquely suited to detect structures and hydrogen bonding networks that are briefly populated, allowing for the characterization of transient conformers in native ensembles. Coupling of HDX to mass spectrometry offers several key advantages, including high sensitivity, low sample consumption and no restriction on protein size...
April 17, 2017: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/28448040/determination-of-high-affinity-antibody-antigen-binding-kinetics-using-four-biosensor-platforms
#2
Danlin Yang, Ajit Singh, Helen Wu, Rachel Kroe-Barrett
Label-free optical biosensors are powerful tools in drug discovery for the characterization of biomolecular interactions. In this study, we describe the use of four routinely used biosensor platforms in our laboratory to evaluate the binding affinity and kinetics of ten high-affinity monoclonal antibodies (mAbs) against human proprotein convertase subtilisin kexin type 9 (PCSK9). While both Biacore T100 and ProteOn XPR36 are derived from the well-established Surface Plasmon Resonance (SPR) technology, the former has four flow cells connected by serial flow configuration, whereas the latter presents 36 reaction spots in parallel through an improvised 6 x 6 crisscross microfluidic channel configuration...
April 17, 2017: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/28448036/using-microfluidic-devices-to-measure-lifespan-and-cellular-phenotypes-in-single-budding-yeast-cells
#3
Ke Zou, Diana S Ren, Qi Ou-Yang, Hao Li, Jiashun Zheng
Budding yeast Saccharomyces cerevisiae is an important model organism in aging research. Genetic studies have revealed many genes with conserved effects on the lifespan across species. However, the molecular causes of aging and death remain elusive. To gain a systematic understanding of the molecular mechanisms underlying yeast aging, we need high-throughput methods to measure lifespan and to quantify various cellular and molecular phenotypes in single cells. Previously, we developed microfluidic devices to track budding yeast mother cells throughout their lifespan while flushing away newborn daughter cells...
March 30, 2017: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/28447798/spontaneous-droplet-motion-on-a-periodic-compliant-substrate
#4
Tianshu Liu, Nichole K Nadermann, Zhenping He, Steven H Strogatz, Chung-Yuen Hui, Anand Jagota
Droplet motion arises in many natural phenomena, ranging from the familiar gravity-driven slip and arrest of raindrops on windows, to the directed transport of droplets for water harvesting by plants and animals under dry conditions. Delib-erate transportation and manipulation of droplets is also important in many technological applications, including drop-let-based microfluidic chemical reactors and for thermal management. Droplet motion usually requires gradients of sur-face energy or temperature, or external vibration to overcome contact angle hysteresis...
April 27, 2017: Langmuir: the ACS Journal of Surfaces and Colloids
https://www.readbyqxmd.com/read/28447797/signal-transductions-of-beas-2b-cells-in-response-to-carcinogenic-pm2-5-exposure-based-on-microfluidic-system
#5
Lulu Zheng, Sixiu Liu, Guoshun Zhuang, Jian Xu, Qi Liu, Xinlian Zhang, Congrui Deng, Zhigang Guo, Wang Zhao, Tingna Liu, Yiqi Wang, Yuxiao Zhang, Jing Lin, Qiongzhen Wang, Guodong Sui
PM2.5 (particulate matter less than 2.5 micrometers in diameter) is considered as a harmful carcinogen. Determining the precise relationship between the chemical constituents of PM2.5 in the air and cancer progression could aid the treatment of environment related disease and establishing risk reduction strategies. Herein, we used transcriptomics (RNA-seq) and integrated microfluidic system to identify the global gene expression and differential target proteins expression induced by ambient fine particles collected from the heavy haze in China...
April 27, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/28447416/microfluidics-in-flow-cytometry-and-related-techniques
#6
REVIEW
M C Béné
Technological advances in laboratory automation are now well understood and applied as they considerably improved the speed and robustness of haematological laboratory data, in the companion fields of blood analyzers and flow cytometry. Still rather confidential is the field of microfluidics, mostly confined so far to academic settings and research laboratories. The literature in the field of microfluidics is growing and applications in hematology range from cell counting to flow cytometry, cell sorting, or ex vivo testing...
May 2017: International Journal of Laboratory Hematology
https://www.readbyqxmd.com/read/28447097/rapid-large-area-fabrication-of-multiscale-through-hole-membranes
#7
Dongha Tahk, Sang-Min Paik, Jungeun Lim, Seokyoung Bang, Soojung Oh, Hyunryul Ryu, Noo Li Jeon
There are many proposed mechanisms by which single cells can be trapped; among them is the through-hole membrane for the characterization of individual microorganisms. Due to the small scale of the fabricated pores, the construction of through-hole membranes on a large scale and with relatively large areas faces many difficulties. This paper describes novel fabrication methods for a large-area, freestanding micro/nano through-hole membrane constructed from versatile membrane materials using through-hole membranes on a microfluidic chip (THMMC)...
April 27, 2017: Lab on a Chip
https://www.readbyqxmd.com/read/28447031/microfluidic-concentric-gradient-generator-design-for-high-throughput-cell-based-studies
#8
Elishai Ezra Tsur, Michal Zimerman, Idan Maor, Avner Elrich, Yaakov Nahmias
Gradients of diffusible signaling molecules play important role in various processes, ranging from cell differentiation to toxicological evaluation. Microfluidic technology provides an accurate control of tempospatial conditions. However, current microfluidic platforms are not designed to handle multiple gradients and cell populations simultaneously. Here, we demonstrate a rapidly adaptable microfluidic design able to expose multiple cell populations to an array of chemical gradients. Our design is based on pressure-equilibrated concentric channels and a pressure-dissipating control layer, facilitating the seeding of multiple cell populations in a single device...
2017: Frontiers in Bioengineering and Biotechnology
https://www.readbyqxmd.com/read/28446964/strategies-for-the-acquisition-of-transcriptional-and-epigenetic-information-in-single-cells
#9
REVIEW
Guang Li, Elda Dzilic, Nick Flores, Alice Shieh, Sean M Wu
As the basic unit of living organisms, each single cell has unique molecular signatures and functions. Our ability to uncover the transcriptional and epigenetic signature of single cells has been hampered by the lack of tools to explore this area of research. The advent of microfluidic single cell technology along with single cell genome-wide DNA amplification methods had greatly improved our understanding of the expression variation in single cells. Transcriptional expression profile by multiplex qPCR or genome-wide RNA sequencing has enabled us to examine genes expression in single cells in different tissues...
March 2017: Journal of Thoracic Disease
https://www.readbyqxmd.com/read/28446924/differential-leukocyte-counting-via-fluorescent-detection-and-image-processing-on-a-centrifugal-microfluidic-platform
#10
Max L Balter, Alvin I Chen, C Amara Colinco, Alexander Gorshkov, Brian Bixon, Vincent Martin, Alexander Fromholtz, Timothy J Maguire, Martin L Yarmush
Centrifugal microfluidics has received much attention in the last decade for the automation of blood testing at the point-of-care, specifically for the detection of chemistries, proteins, and nucleic acids. However, the detection of common blood cells on-disc, particularly leukocytes, remains a challenge. In this paper, we present two analytical methods for enumerating leukocytes on a centrifugal platform using a custom-built fluorescent microscope, acridine orange nuclear staining, and image processing techniques...
December 21, 2016: Analytical Methods: Advancing Methods and Applications
https://www.readbyqxmd.com/read/28446756/a-chemically-patterned-microfluidic-paper-based-analytical-device-c-%C3%A2%C2%B5pad-for-point-of-care-diagnostics
#11
Trinh Lam, Jasmine P Devadhasan, Ryan Howse, Jungkyu Kim
A chemically patterned microfluidic paper-based analytical device (C-µPAD) is developed to create fluidic networks by forming hydrophobic barriers using chemical vapor deposition (CVD) of trichlorosilane (TCS) on a chromatography paper. By controlling temperature, pattern size, and CVD duration, optimal conditions were determined by characterizing hydrophobicity, spreading patterns, and flow behavior on various sized fluidic patterns. With these optimal conditions, we demonstrated glucose assay, immunoassay, and heavy metal detection on well-spot C-µPAD and lateral flow C-µPAD...
April 26, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28446020/direct-writing-of-microfluidic-footpaths-by-pyro-ehd-printing
#12
Sara Coppola, Giuseppe Nasti, Michele Todino, Federico Olivieri, Veronica Vespini, Pietro Ferraro
A direct writing method for fabricating microfluidic footpaths by pyro-Electrohydrodynamic (EHD) jet printing is reported. Here we propose the use of a nozzle-free 3D printing technique for the fabrication of printed structures that can be embedded in a variety of soft, transparent, flexible and biocompatible polymers, and thus easily integrated into lab-on-chip (LOC) devices. We prove the advantage of the high resolution and flexibility of pyro-EHD printing for the realization of microfluidic channels well below the standard limit in dimension of conventional ink-jet printing technique and simply adaptable to the final user desires in terms of geometry and materials...
April 27, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/28446009/applications-of-microfluidic-devices-for-urology
#13
REVIEW
Se Jik Han, Hun-Kuk Park, Kyung Sook Kim
Microfluidics is considered an important technology that is suitable for numerous biomedical applications, including cancer diagnosis, metastasis, drug delivery, and tissue engineering. Although microfluidics is still considered to be a new approach in urological research, several pioneering studies have been reported in recent years. In this paper, we reviewed urological research works using microfluidic devices. Microfluidic devices were used for the detection of prostate and bladder cancer and the characterization of cancer microenvironments...
April 2017: International Neurourology Journal
https://www.readbyqxmd.com/read/28445813/microfluidic-integrated-patterned-ito-immunosensor-for-rapid-detection-of-prostate-specific-membrane-antigen-biomarker-in-prostate-cancer
#14
Rajesh Seenivasan, Chandra K Singh, Jay W Warrick, Nihal Ahmad, Sundaram Gunasekaran
An optically transparent patterned indium tin oxide (ITO) three-electrode sensor integrated with a microfluidic channel was designed for label-free immunosensing of prostate-specific membrane antigen (PSMA), a prostate cancer (PCa) biomarker, expressed on prostate tissue and circulating tumor cells but also found in serum. The sensor relies on cysteamine capped gold nanoparticles (N-AuNPs) covalently linked with anti-PSMA antibody (Ab) for target specificity. A polydimethylsiloxane (PDMS) microfluidic channel is used to efficiently and reproducibly introduce sample containing soluble proteins/cells to the sensor...
April 11, 2017: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/28445605/high-throughput-cell-mechanical-phenotyping-for-label-free-titration-assays-of-cytoskeletal-modifications
#15
Stefan Golfier, Philipp Rosendahl, Alexander Mietke, Maik Herbig, Jochen Guck, Oliver Otto
The mechanical fingerprint of cells is inherently linked to the structure of the cytoskeleton and can serve as a label free marker for cell homeostasis or pathologic states. How cytoskeletal composition affects the physical response of cells to external loads has been intensively studied with a spectrum of techniques, yet quantitative and statistically powerful investigations in the form of titration assays are hampered by the low throughput of most available methods. In this study, we employ real-time deformability cytometry (RT-DC), a novel microfluidic tool to examine the effects of biochemically modified F-actin and microtubule stability and nuclear chromatin structure on cell deformation in a human leukemia cell line (HL60)...
April 26, 2017: Cytoskeleton
https://www.readbyqxmd.com/read/28445168/enhanced-photoluminescence-of-corrugated-al2o3-film-assisted-by-colloidal-cdse-quantum-dots
#16
Zhongchen Bai, Licai Hao, Zhengping Zhang, Zhaoling Huang, Shuijie Qin
We present the enhanced photoluminescence (PL) of a corrugated Al2O3 film enabled by colloidal CdSe quantum dots. The colloidal CdSe quantum dots are fabricated directly on a corrugated Al2O3 substrate using an electrochemical deposition (ECD) method in a microfluidic system. The photoluminescence is excited by using a 150 nm diameter ultraviolet laser spot of a scanning near-field optical microscope. Owing to the electron transfer from the conduction band of the CdSe quantum dots to that of Al2O3, the enhanced photoluminescence effect is observed, which results from the increase in the recombination rate of electrons and holes on the Al2O3 surface and the reduction in the fluorescence of the CdSe quantum dots...
May 19, 2017: Nanotechnology
https://www.readbyqxmd.com/read/28445076/image-based-phenotypic-screening-with-human-primary-t-cells-using-one-dimensional-imaging-cytometry-with-self-tuning-statistical-gating-algorithms
#17
Steve S Wang, Daniel J Ehrlich
The parallel microfluidic cytometer (PMC) is an imaging flow cytometer that operates on statistical analysis of low-pixel-count, one-dimensional (1D) line scans. It is highly efficient in data collection and operates on suspension cells. In this article, we present a supervised automated pipeline for the PMC that minimizes operator intervention by incorporating multivariate logistic regression for data scoring. We test the self-tuning statistical algorithms in a human primary T-cell activation assay in flow using nuclear factor of activated T cells (NFAT) translocation as a readout and readily achieve an average Z' of 0...
April 1, 2017: SLAS Discovery
https://www.readbyqxmd.com/read/28443874/inertial-migration-and-axial-control-of-deformable-capsules
#18
Christian Schaaf, Holger Stark
The mechanical deformability of single cells is an important indicator for various diseases such as cancer, blood diseases and inflammation. Lab-on-a-chip devices allow to separate such cells from healthy cells using hydrodynamic forces. We perform hydrodynamic simulations based on the lattice-Boltzmann method and study the behavior of an elastic capsule in a microfluidic channel flow in the inertial regime. While inertial lift forces drive the capsule away from the channel center, its deformability favors migration in the opposite direction...
April 26, 2017: Soft Matter
https://www.readbyqxmd.com/read/28443860/fabrication-of-large-area-polymer-microfilter-membranes-and-their-application-for-particle-and-cell-enrichment
#19
Javier Alejandro Hernández-Castro, Kebin Li, Anne Meunier, David Juncker, Teodor Veres
A vacuum assisted UV micro-molding (VAUM) process is proposed for the fabrication of freestanding and defect-free polymer membranes based on a UV-curable methacrylate polymer (MD 700). VAUM is a highly flexible and powerful method for fabricating low cost, robust, large-area membranes over 9 × 9 cm(2) with pore sizes from 8 to 20 μm in diameter, 20 to 100 μm in thickness, high aspect ratio (the thickness of the polymer over the diameter of the hole is up to 15 : 1), high porosity, and a wide variety of geometrical characteristics...
April 26, 2017: Lab on a Chip
https://www.readbyqxmd.com/read/28443845/passive-and-active-colloidal-chemotaxis-in-a-microfluidic-channel-mesoscopic-and-stochastic-models
#20
Laurens Deprez, Pierre de Buyl
Chemotaxis is the response of a particle to a gradient in the chemical composition of the environment. While it was originally observed for biological organisms, it is of great interest in the context of synthetic active particles such as nanomotors. Experimental demonstration of chemotaxis for chemically-powered colloidal nanomotors was reported in the literature in the context of chemo-attraction in a still fluid or in a microfluidic channel where the gradient is sustained by a specific inlet geometry. In this work, we use mesoscopic particle-based simulations of the colloid and solvent to demonstrate chemotaxis in a microfluidic channel...
April 26, 2017: Soft Matter
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