Diandra Doppler, Mukul Sonker, Ana Egatz-Gomez, Alice Grieco, Sahba Zaare, Rebecca Jernigan, Jose Domingo Meza-Aguilar, Mohammad T Rabbani, Abhik Manna, Roberto C Alvarez, Konstantinos Karpos, Jorvani Cruz Villarreal, Garrett Nelson, Jay-How Yang, Jackson Carrion, Katherine Morin, Gihan K Ketawala, Angel L Pey, Miguel Angel Ruiz-Fresneda, Juan Luis Pacheco-Garcia, Juan A Hermoso, Reza Nazari, Raymond Sierra, Mark S Hunter, Alexander Batyuk, Christopher J Kupitz, Robert E Sublett, Stella Lisova, Valerio Mariani, Sébastien Boutet, Raimund Fromme, Thomas D Grant, Sabine Botha, Petra Fromme, Richard A Kirian, Jose Manuel Martin-Garcia, Alexandra Ros
Droplet injection strategies are a promising tool to reduce the large amount of sample consumed in serial femtosecond crystallography (SFX) measurements at X-ray free electron lasers (XFELs) with continuous injection approaches. Here, we demonstrate a new modular microfluidic droplet injector (MDI) design that was successfully applied to deliver microcrystals of the human NAD(P)H:quinone oxidoreductase 1 (NQO1) and phycocyanin. We investigated droplet generation conditions through electrical stimulation for both protein samples and implemented hardware and software components for optimized crystal injection at the Macromolecular Femtosecond Crystallography (MFX) instrument at the Stanford Linac Coherent Light Source (LCLS)...
June 9, 2023: Lab on a Chip