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https://www.readbyqxmd.com/read/27905550/deep-sequencing-of-transcriptome-profiling-of-gstm2-knock-down-in-swine-testis-cells
#1
Yuqi Lv, Yi Jin, Yongqiang Zhou, Jianjun Jin, Zhenfa Ma, Zhuqing Ren
Glutathione-S-transferases mu 2 (GSTM2), a kind of important Phase II antioxidant enzyme of eukaryotes, is degraded by nonsense mediated mRNA decay due to a C27T substitution in the fifth exon of pigs. As a reproductive performance-related gene, GSTM2 is involved in embryo implantation, whereas, functional deficiency of GSTM2 induces pre- or post-natal death in piglets potentially. To have some insight into the role of GSTM2 in embryo development, high throughput RNA sequencing is performed using the swine testis cells (ST) with the deletion of GSTM2...
December 1, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27707917/identification-of-ifn-stimulated-gene-isg-proteins-that-inhibit-human-parainfluenza-virus-type-3
#2
M A G Rabbani, Michael Ribaudo, Ju-Tao Guo, Sailen Barik
: A major arm of cellular innate immunity is type I interferon (IFN), represented by IFN-α and IFN-β. Type I IFN transcriptionally induces a large number of cellular genes, collectively known as IFN-Stimulated Genes or ISGs, which act as antivirals. The IFIT (interferon-induced proteins with tetratricopeptide repeats)-family proteins constitute a major subclass of ISG, and are characterized by multiple tetratricopeptide repeats (TPRs). Here, we have interrogated IFIT proteins for their ability to inhibit the growth of human parainfluenza virus type 3 (PIV3), a nonsegmented negative-strand RNA virus of the Paramyxoviridae family and major cause of respiratory disease in children...
October 5, 2016: Journal of Virology
https://www.readbyqxmd.com/read/27275413/comprehensive-and-computational-analysis-of-genes-in-human-umbilical-vein-endothelial-cells-responsive-to-x-irradiation
#3
Yukihiro Furusawa, Qing-Li Zhao, Yuichi Hattori, Yoshiaki Tabuchi, Toshiyasu Iwasaki, Takaharu Nomura, Takashi Kondo
Radiation exposure such as A-bomb or radiation therapy is considered a major health-risk factor for cardiovascular disease. In order to understand the molecular mechanisms underlying the inflammatory reaction frequently encountered in the vascular system after exposure to ionizing radiation, we carried out a global scale microarray and computational gene expression analyses on human umbilical endothelial cells (HUVECs) exposed to X-ray (2.5 Gy). The gene ontology analysis revealed that the down-regulated genes were associated with cell cycle regulation, whereas the up-regulated genes were associated with inflammatory responses, in particular, the type 1 interferon response...
June 2016: Genomics Data
https://www.readbyqxmd.com/read/27244557/phylogenetic-sleuthing-reveals-pair-of-paralogous-genes
#4
Jamie E Henzy, Welkin E Johnson
The complex evolutionary history of the IFIT family of antiviral genes has been shaped by continuous interactions between mammalian hosts and their many viruses.
2016: ELife
https://www.readbyqxmd.com/read/27240734/evolution-guided-functional-analyses-reveal-diverse-antiviral-specificities-encoded-by-ifit1-genes-in-mammals
#5
Matthew D Daugherty, Aaron M Schaller, Adam P Geballe, Harmit S Malik
IFIT (interferon-induced with tetratricopeptide repeats) proteins are critical mediators of mammalian innate antiviral immunity. Mouse IFIT1 selectively inhibits viruses that lack 2'O-methylation of their mRNA 5' caps. Surprisingly, human IFIT1 does not share this antiviral specificity. Here, we resolve this discrepancy by demonstrating that human and mouse IFIT1 have evolved distinct functions using a combination of evolutionary, genetic and virological analyses. First, we show that human IFIT1 and mouse IFIT1 (renamed IFIT1B) are not orthologs, but are paralogs that diverged >100 mya...
2016: ELife
https://www.readbyqxmd.com/read/27137933/interferon-regulatory-factor-8-irf8-impairs-induction-of-interferon-induced-with-tetratricopeptide-repeat-motif-ifit-gene-family-members
#6
Christine L White, Patricia M Kessler, Benjamin K Dickerman, Keiko Ozato, Ganes C Sen
The chromosomally clustered interferon-induced with tetratricopeptide repeat motif (IFIT) gene family members share structural features at the gene and protein levels. Despite these similarities, different IFIT genes have distinct inducer- and cell type-specific induction patterns. Here, we investigated the mechanism for the observed differential induction of the mouse Ifit1, Ifit2, and Ifit3 genes in B cells and demonstrated that the repressive effect of the transcription factor interferon regulatory factor 8 (IRF8), which is highly expressed in B cells, played an essential role in this regulation...
June 24, 2016: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/27135915/distinct-expression-of-interferon-induced-protein-with-tetratricopeptide-repeats-ifit-1-2-3-and-other-antiviral-genes-between-subsets-of-dendritic-cells-induced-by-dengue-virus-2-infection
#7
Jingshu Zhang, Daniel Man-Yuen Sze, Benjamin Yat-Ming Yung, Petrus Tang, Wei-June Chen, Kwok-Hung Chan, Polly Hang-Mei Leung
Dengue virus (DENV) infection is an emerging public health hazard threatening inhabitants of the tropics and sub-tropics. Dendritic cells (DCs) are one of the major targets of DENV and the initiators of the innate immune response against the virus. However, current in vitro research on the DENV-DC interaction is hampered by the low availability of ex vivo DCs and donor variation. In the current study, we attempted to develop a novel in vitro DC model using immature DCs derived from the myeloid leukaemia cell line MUTZ-3 (IMDCs) to investigate the DENV-DC interaction...
August 2016: Immunology
https://www.readbyqxmd.com/read/27060565/the-south-pacific-epidemic-strain-of-zika-virus-replicates-efficiently-in-human-epithelial-a549-cells-leading-to-ifn-%C3%AE-production-and-apoptosis-induction
#8
Etienne Frumence, Marjolaine Roche, Pascale Krejbich-Trotot, Chaker El-Kalamouni, Brice Nativel, Philippe Rondeau, Dorothée Missé, Gilles Gadea, Wildriss Viranaicken, Philippe Desprès
Zika virus (ZIKV) is an emerging flavivirus since the first epidemics in South Pacific in 2007. The recent finding that ZIKV is now circulating in Western Hemisphere and can be associated to severe human diseases, warrants the need for its study. Here we evaluate the susceptibility of human lung epithelial A549 cells to South Pacific epidemic strain of ZIKV isolated in 2013. We showed that ZIKV growth in A549 cells is greatly efficient. ZIKV infection resulted in the secretion of IFN-β followed by the expression of pro-inflammatory cytokines such as IL-1β, and transcriptional activity of IFIT genes...
June 2016: Virology
https://www.readbyqxmd.com/read/26916433/when-your-cap-matters-structural-insights-into-self-vs-non-self-recognition-of-5-rna-by-immunomodulatory-host-proteins
#9
REVIEW
Daisy W Leung, Gaya K Amarasinghe
Cytosolic recognition of viral RNA is important for host innate immune responses. Differential recognition of self vs non-self RNA is a considerable challenge as the inability to differentiate may trigger aberrant immune responses. Recent work identified the composition of the RNA 5', including the 5' cap and its methylation state, as an important determinant of recognition by the host. Recent studies have advanced our understanding of the modified 5' RNA recognition and viral antagonism of RNA receptors. Here, we will discuss RIG-I and IFIT proteins as examples of host proteins that detect dsRNA and ssRNA, respectively...
February 2016: Current Opinion in Structural Biology
https://www.readbyqxmd.com/read/26423178/interferon-stimulated-gene-isg-60-as-well-as-isg56-and-isg54-positively-regulates-tlr3-ifn-%C3%AE-stat1-axis-in-u373mg-human-astrocytoma-cells
#10
Tadaatsu Imaizumi, Hidemi Yoshida, Ryo Hayakari, Fei Xing, Lian Wang, Tomoh Matsumiya, Kunikazu Tanji, Shogo Kawaguchi, Manabu Murakami, Hiroshi Tanaka
Treatment of cells with interferons (IFNs) induces the phosphorylation of signal transducer and activator of transcription 1 (STAT1), leading to the expression of hundreds of IFN-stimulated genes (ISGs). ISGs exert various antiviral and pro-inflammatory reactions. We have previously reported that ISG56 and ISG54 are induced by polyinosinic-polycytidylic acid (poly IC), an authentic agonist for Toll-like receptor 3 (TLR3), in U373MG human astrocytoma cells. ISG56 and ISG54 are also named as IFN-induced proteins with tetratricopeptide repeats (IFIT) 1 and IFIT2, respectively...
April 2016: Neuroscience Research
https://www.readbyqxmd.com/read/26053856/differential-expression-profile-of-chicken-embryo-fibroblast-df-1-cells-infected-with-cell-adapted-infectious-bursal-disease-virus
#11
Raymond K Hui, Frederick C Leung
RNA-Seq was used to unveil the transcriptional profile of DF-1 cells at the early stage of caIBDV infection. Total RNAs were extracted from virus-infected cells at 0, 6 and 12 hpi. RNA-Seq datasets of respective samples mapped to 56.5-57.6% of isoforms in the reference genome Galgal4.73. At 6 hpi, 23 isoforms underwent an elevated expression, while 128 isoforms were up-regulated and 5 were down-regulated at 12 hpi in the virus-infected group. Besides, 10 isoforms were exclusively expressed in the virus-infected cells...
2015: PloS One
https://www.readbyqxmd.com/read/26021086/-inhibition-of-replication-and-transcription-of-wsn-influenza-a-virus-by-ifit-family-genes
#12
Lidan Hou, Jing Li, Hongren Qu, Limin Yang, Yajun Chen, Qianqian Du, Wenjun Liu
IFIT family genes are a kind of interferon stimulated genes (ISGs), and play important roles in antiviral sector and immunity regulation. To study the regulatory effect of IFIT family genes during influenza A virus (IAV) infection, we used RNA-sequencing analysis (RNA-Seq) technique and found that when 293T cells were infected by A/WSN/33 (WSN), the concentration of IFIT family genes were increased. Further study reveals that overexpression of IFIT2 or IFIT3 could inhibit IAV replication and transcription, and cause the dose-dependent inhibition of polymerase activity of vRNP...
January 2015: Sheng Wu Gong Cheng Xue Bao, Chinese Journal of Biotechnology
https://www.readbyqxmd.com/read/25923090/prevalence-of-anti-thyroid-peroxidase-in-adults-with-type-1-diabetes-participating-in-pozna%C3%A5-prospective-study
#13
Agata Grzelka, Aleksandra Araszkiewicz, Aleksandra Uruska, Dorota Zozulińska-Ziółkiewicz
BACKGROUND: Type 1 diabetes (DM 1) is frequently associated with autoimmune thyroid diseases (AITD). Screening for AITD in adults is rarely performed. The aim of this study was to evaluate the prevalence of anti-thyroid peroxidase (anti-TPO) and thyroid function and their association with metabolic control in adults participating in Poznan Prospective Study (PoProStu). MATERIAL AND METHODS: The analysis included 74 patients (26 women and 48 men) aged 38.5 (IQR: 34...
January 2015: Advances in Clinical and Experimental Medicine: Official Organ Wroclaw Medical University
https://www.readbyqxmd.com/read/25905045/comparative-proteomic-analyses-demonstrate-enhanced-interferon-and-stat-1-activation-in-reovirus-t3d-infected-hela-cells
#14
COMPARATIVE STUDY
Peyman Ezzati, Krysten Komher, Giulia Severini, Kevin M Coombs
As obligate intracellular parasites, viruses are exclusively and intimately dependent upon their host cells for replication. During replication viruses induce profound changes within cells, including: induction of signaling pathways, morphological changes, and cell death. Many such cellular perturbations have been analyzed at the transcriptomic level by gene arrays and recent efforts have begun to analyze cellular proteomic responses. We recently described comparative stable isotopic (SILAC) analyses of reovirus, strain type 3 Dearing (T3D)-infected HeLa cells...
2015: Frontiers in Cellular and Infection Microbiology
https://www.readbyqxmd.com/read/25902155/dengue-virus-infection-of-primary-endothelial-cells-induces-innate-immune-responses-changes-in-endothelial-cells-function-and-is-restricted-by-interferon-stimulated-responses
#15
Julie K Calvert, Karla J Helbig, David Dimasi, Michaelia Cockshell, Michael R Beard, Stuart M Pitson, Claudine S Bonder, Jillian M Carr
Although endothelial cell (EC) infection is not widespread during dengue virus (DENV) infection in vivo, the endothelium is the site of the pathogenic effects seen in severe DENV disease. In this study, we investigated DENV infection of primary EC and defined factors that influence infection in this cell type. Consistent with in vivo findings where EC infection is infrequent, only 3%-15% of EC became productively DENV-2-infected in vitro. This low level infection could not be attributed to inhibition by heparin, EC donor variation, heterogeneity, or biological source...
August 2015: Journal of Interferon & Cytokine Research
https://www.readbyqxmd.com/read/25889446/mir-146a-suppresses-cellular-immune-response-during-japanese-encephalitis-virus-jaoars982-strain-infection-in-human-microglial-cells
#16
Nikhil Sharma, Ruhi Verma, Kanhaiya Lal Kumawat, Anirban Basu, Sunit K Singh
BACKGROUND: Japanese encephalitis virus (JEV) is the causative agent of Japanese encephalitis which is more prevalent in South and Southeast Asia. JEV is a neurotropic virus which infiltrates into the brain through vascular endothelial cells. JEV infects neurons and microglial cells which causes neuronal damage and inflammation. However, JEV also evades the cellular immune response to survive in host cells. Viruses are known to modulate the expression of microRNAs, which in turn modulate cellular immune response by targeting expression of antiviral genes...
2015: Journal of Neuroinflammation
https://www.readbyqxmd.com/read/25816333/identification-and-expression-analysis-of-the-interferon-induced-protein-with-tetratricopeptide-repeats-5-ifit5-gene-in-duck-anas-platyrhynchos-domesticus
#17
Bin Wang, Yang Chen, Chunyu Mu, Yanhui Su, Ran Liu, Zhengyang Huang, Yang Li, Qingming Yu, Guobin Chang, Qi Xu, Guohong Chen
The interferon-induced proteins with tetratricopeptide repeats (IFITs) protein family mediates antiviral effects by inhibiting translation initiation, cell proliferation, and migration in the interferon (IFN) dependent innate immune system. Several members of this family, including IFIT1, IFIT2, IFIT3 and IFIT5, have been heavily studied in mammals. Avian species contain only one family member, IFIT5, and little is known about the role of this protein in birds. In this study, duck IFIT5 (duIFIT5) full-length mRNA was cloned by reverse transcription polymerase chain reaction (RT-PCR) and rapid amplification of the cDNA ends (RACE)...
2015: PloS One
https://www.readbyqxmd.com/read/25779435/mir-146a-suppresses-cellular-immune-response-during-japanese-encephalitis-virus-jaoars982-strain-infection-in-human-microglial-cells
#18
Nikhil Sharma, Ruhi Verma, Kanhaiya Lal Kumawat, Anirban Basu, Sunit K Singh
BACKGROUND: Japanese encephalitis virus (JEV) is the causative agent of Japanese encephalitis which is more prevalent in South and Southeast Asia. JEV is a neurotropic virus which infiltrates into the brain through vascular endothelial cells. JEV infects neurons and microglial cells which causes neuronal damage and inflammation. However, JEV also evades the cellular immune response to survive in host cells. Viruses are known to modulate the expression of microRNAs, which in turn modulate cellular immune response by targeting expression of antiviral genes...
December 2015: Journal of Neuroinflammation
https://www.readbyqxmd.com/read/25772570/promotion-of-expression-of-interferon-stimulated-genes-in-u937-monocytic-cells-by-hiv-rnas-measured-using-stable-isotope-labeling-with-amino-acids-in-cell-culture-silac
#19
Yulan Li, Bin Wen, Ran Chen, Feng Jiang, Xiaofang Zhao, Xin Deng
Type I interferon (IFN) exerts strong antiviral activity, particularly against human immunodeficiency virus (HIV), and although several viral proteins have been shown to deregulate IFN induction, little is known about the induction of type I IFNs by HIV RNAs. In the present study, we used the stable isotope labeling with amino acids in cell culture (SILAC) method to determine the proteomic profile in U937 monocytic cells after transfection with viral RNA of HIV. We then used a western blot assay to validate the proteomic results...
May 2015: Archives of Virology
https://www.readbyqxmd.com/read/25428874/interferon-induced-ifit-proteins-their-role-in-viral-pathogenesis
#20
REVIEW
Volker Fensterl, Ganes C Sen
A major component of the protective antiviral host defense is contributed by the intracellular actions of the proteins encoded by interferon-stimulated genes (ISGs); among these are the interferon-induced proteins with tetratricopeptide repeats (IFITs), consisting of four members in human and three in mouse. IFIT proteins do not have any known enzyme activity. Instead, they inhibit virus replication by binding and regulating the functions of cellular and viral proteins and RNAs. Although all IFITs are comprised of multiple copies of the degenerate tetratricopeptide repeats, their distinct tertiary structures enable them to bind different partners and affect host-virus interactions differently...
March 2015: Journal of Virology
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