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Immunohistochemistry for hepatic metastatic tumors

Alex D Michaels, Timothy E Newhook, Sara J Adair, Sho Morioka, Bernadette J Goudreau, Sarbajeet Nagdas, Matthew G Mullen, Jesse B Persily, Timothy N J Bullock, Craig L Slingluff, Kodi S Ravichandran, J Thomas Parsons, Todd W Bauer
Purpose: Patients with pancreatic ductal adenocarcinoma (PDAC) who undergo surgical resection and adjuvant chemotherapy have an expected survival of only 2 years due to disease recurrence, frequently in the liver. We investigated the role of liver macrophages in progression of PDAC micrometastases to identify adjuvant treatment strategies that could prolong survival. Experimental Design: A murine splenic injection model of hepatic micrometastatic PDAC was used with five patient-derived PDAC tumors. The impact of liver macrophages on tumor growth was assessed by (i) depleting mouse macrophages in nude mice with liposomal clodronate injection, and (ii) injecting tumor cells into nude versus NOD- scid -gamma mice...
March 15, 2018: Clinical Cancer Research: An Official Journal of the American Association for Cancer Research
Jessica Beck, Margaret A Miller, Chad Frank, Dee DuSold, José Antonio Ramos-Vara
Thyroid transcription factor-1 (TTF-1) is a specific and sensitive marker for canine pulmonary tumors but is also expressed in thyroid carcinomas, which commonly metastasize to lung. Napsin A and surfactant protein A (SP-A) are used in the histologic diagnosis of non-small-cell lung cancer in humans but have not been thoroughly evaluated in neoplasms of dogs. The objective of this study was to compare the efficacy of immunohistochemistry for SP-A, napsin A, and TTF-1 in the diagnosis of canine pulmonary carcinomas...
January 1, 2017: Veterinary Pathology
Veronica Prieto, Johannes M Ludwig, Alton B Farris, Ganji Purnachandra Nagaraju, Taoreed O Lawal, Bassel El-Rayes, Hyun S Kim
RATIONALE AND OBJECTIVES: To develop a human metastatic colorectal cancer (mCRC) model in a rabbit liver. MATERIALS AND METHODS: Immunosuppression in 4 adult New Zealand White rabbits weighing 3.5 to 4.5 kg was induced with daily subcutaneous injection of 15 mg/kg Cyclosporine A (CsA). On day 3 open mini-laparotomy was performed and 0.2 ml (1.8x105 cells) suspension of HCT-116 and HT-29 human CRC cells were injected into the left and right medial lobe respectively...
2017: PloS One
Hidefumi Kubo, Hitoshi Yamazaki, Takemichi Okada, Yoshihito Takahashi, Yatsushi Nishi, Hiroaki Yokomori
BACKGROUND: Hepatic angiomyolipomas are a rare, benign group of mesenchymal tumors in the liver. Hepatic angiomyolipoma is sometimes misdiagnosed as hepatocellular carcinoma, and there is the possibility of a malignant transformation. Hence, the accurate diagnosis of this disorder is necessary. CASE PRESENTATION: A 64-year-old Japanese man was observed to have a space-occupying lesion of 15-mm diameter in the liver during a follow-up examination for a previously resected cecal carcinoma...
March 22, 2017: Journal of Medical Case Reports
Xia Ying, Su-Xia Han, Chen-Chen He, Cong-Ya Zhou, Yi-Ping Dong, Meng-Jiao Cai, Xin Sui, Cheng-Xian Ma, Xiao Sun, Yuan-Yuan Zhang, Wen-Li Gou, Clifford Mason, Qing Zhu
PURPOSE: To identify Heptocellular carcinoma (HCC) associated antigens by proteomics, and validate whether autoantibodies against tumor-associated antigens (TAAs) could be used for diagnosis and conditional monitoring. RESULTS: The 78 kDa glucose regulated protein (GRP78) was selected as a candidate TAA. The titers of autoantibodies against 78 kDa glucose regulated protein (GRP78) from patients with HCC, liver cirrhosis (LC), and chronic hepatitis (CH) were significantly higher than that from normal controls (P<0...
April 11, 2017: Oncotarget
Jonathan R Brody, Cinthya S Yabar, Mahsa Zarei, Joseph Bender, Lynn M Matrisian, Lola Rahib, Craig Heartwell, Kimberly Mason, Charles J Yeo, Stephen C Peiper, Wei Jiang, Katelyn Varieur, Subha Madhavan, Emanuel Petricoin, Danielle Fortuna, Mark Curtis, Zi-Xuan Wang, Michael J Pishvaian, Jordan M Winter
Isocitrate dehydrogenase 1 (IDH1) is a metabolic enzyme implicated in cancer cell metabolic reprogramming. This is underscored by the detection of functional, somatic IDH1 mutations frequently found in secondary glioblastoma. To our knowledge, there has never been a reported, validated case of an IDH1 mutation in a pancreatic ductal adenocarcinoma (PDA). Herein, we present a case of a patient with metastatic PDA that harbored a potentially actionable, albeit rare, IDH1 mutation. As part of the Know Your Tumor project (Pancreatic Cancer Action Network), a 48-year-old female was diagnosed with metastatic PDA and subsequently started on standard of care chemotherapy, during which her hepatic lesions progressed...
July 28, 2016: Cancer Biology & Therapy
David C Qian, Xiangjun Xiao, Jinyoung Byun, Arief A Suriawinata, Stephanie C Her, Christopher I Amos, Richard J Barth
PURPOSE: We have previously demonstrated that patients with metastatic colorectal cancer who exhibit immune responses to a dendritic cell (DC) vaccine have superior recurrence-free survival following surgery, compared with patients in whom responses do not occur. We sought to characterize the patterns of T-lymphocyte infiltration and somatic mutations in metastases that are associated with and predictive of response to the DC vaccine. EXPERIMENTAL DESIGN: Cytotoxic, memory, and regulatory T cells in resected metastases and surrounding normal liver tissue from 22 patients (11 responders and 11 nonresponders) were enumerated by immunohistochemistry prior to vaccine administration...
January 15, 2017: Clinical Cancer Research: An Official Journal of the American Association for Cancer Research
Hannes H Brandt, Valérie Nißler, Roland S Croner
The high incidence of tumor recurrence after resection of metastatic liver lesions remains an unsolved problem. Small tumor cell deposits, which are not detectable by routine clinical imaging, may be stimulated by hepatic regeneration factors after liver resection. It is not entirely clear, however, which factors are crucial for tumor recurrence. The presented mouse model may be useful to explore the mechanisms that play a role in the development of recurrent malignant lesions after liver resection. The model combines the easy-to-perform and reproducible techniques of defined amounts of liver tissue removal and tumor induction (by injection) in mice...
April 9, 2016: Journal of Visualized Experiments: JoVE
Alice Bartolini, Sabrina Cardaci, Simona Lamba, Daniele Oddo, Caterina Marchiò, Paola Cassoni, Carla Azzurra Amoreo, Giorgio Corti, Alessandro Testori, Federico Bussolino, Renata Pasqualini, Wadih Arap, Davide Corà, Federica Di Nicolantonio, Serena Marchiò
PURPOSE: KRAS mutations confer adverse prognosis to colorectal cancer, and no targeted therapies have shown efficacy in this patient subset. Paracrine, nongenetic events induced by KRAS-mutant tumor cells are expected to result in specific deregulation and/or relocation of tumor microenvironment (TME) proteins, which in principle can be exploited as alternative therapeutic targets. EXPERIMENTAL DESIGN: A multimodal strategy combining ex vivo/in vitro phage display screens with deep-sequencing and bioinformatics was applied to uncover TME-specific targets in KRAS-mutant hepatic metastasis from colorectal cancer...
October 1, 2016: Clinical Cancer Research: An Official Journal of the American Association for Cancer Research
Jianhua Liu, Haosheng Jin, Hongxia Tian, Guoda Lian, Shaojie Chen, Jiayu Li, Xuchao Zhang, Dong Ma
The present study aimed to investigate anaplastic lymphoma kinase (ALK) status in hepatocellular carcinoma (HCC) and to evaluate whether abnormalities in expression were associated with patient prognosis. ALK status was investigated using immunohistochemistry (IHC), reverse transcription-quantitative polymerase chain reaction (RT-qPCR) and fluorescence in situ hybridization (FISH) assays in 342 HCC patients. In addition, rapid amplification of complementary DNA ends-coupled PCR sequencing was performed, in order to confirm the presence of ALK abnormalities in patients exhibiting ALK messenger RNA (mRNA) overexpression...
January 2016: Oncology Letters
Yongkook Kim, Yohei Hosoda, Masaya Nishino, Miho Okano, Junji Kawada, Masaru Yamasaki, Ken-ichi Nagai, Masayosi Yasui, Masaki Okuyama, Toshimasa Tsujinaka
A 62-year-old man was diagnosed with gastric cancer and underwent distal gastrectomy, and D1+b lymph node dissection. He was diagnosed postoperatively with T1b (sm2) N0M0, StageⅠA gastric adenocarcinoma and did not receive any adjuvant chemotherapy after surgery. One year and 6 months after gastrectomy, blood analysis indicated high levels of carcinoembryonic antigen (CEA 262.1 ng/mL) while abdominal computed tomography (CT) revealed multiple liver tumors (S7: 15 mm, S7/8: 20 mm). The patient was diagnosed with metachronous multiple liver metastases from gastric cancer...
November 2015: Gan to Kagaku Ryoho. Cancer & Chemotherapy
Michele Diana, Eric Robinet, Yu-Yin Liu, Andras Legnèr, Seong-Ho Kong, Luigi Schiraldi, Francesco Marchegiani, Peter Halvax, Lee Swanstrom, Bernard Dallemagne, Jacques Marescaux
BACKGROUND: Tumor-specific fluorescent antibodies, which can be recognized at a cellular or tissue level using optical imaging such as confocal laser endomicroscopy (CLE), could provide a means for rapid and accurate tumor diagnosis and staging. The aim of this study was to evaluate the ability of CLE to detect the presence of tagged cells within lymph nodes in an original simulated metastatic model. MATERIALS AND METHODS: A solution of indocyanine green containing a suspension of porcine hepatocytes, marked with carboxy-fluorescein-succinimidyl-ester (CFSE), was injected endoscopically in the gastric submucosa of 10 pigs...
December 2016: Annals of Surgical Oncology
Eran Sadot, Bas Groot Koerkamp, Julie N Leal, Jinru Shia, Mithat Gonen, Peter J Allen, Ronald P DeMatteo, T Peter Kingham, Nancy Kemeny, Leslie H Blumgart, William R Jarnagin, Michael I DʼAngelica
OBJECTIVES: The impact of margin width on overall survival (OS) in the context of other prognostic factors after resection for colorectal liver metastases is unclear. We evaluated the relationship between resection margin and OS utilizing high-resolution histologic distance measurements. METHODS: A single-institution prospectively maintained database was queried for all patients who underwent an initial complete resection of colorectal liver metastases between 1992 and 2012...
September 2015: Annals of Surgery
Kumiko Tatsuda, Tatsuo Kanda, Takashi Ishikawa, Seiichi Hirota, Ken Nishikura, Kazuhito Yajima, Shin-Ichi Kosugi, Katsuyoshi Hatakeyama
It is unknown how long the risk of developing secondary resistance to imatinib persists in patients with gastrointestinal stromal tumors (GISTs). Here we report a case of a patient with a metastatic gastric GIST who developed secondary resistance to imatinib 70 months after initiation of imatinib therapy. A 62-year-old woman with a gastric GIST underwent total gastrectomy with pancreaticosplenectomy. Immunohistochemistry revealed a KIT-positive GIST. The mitotic index of the tumor was 13/50 high-power fields, indicating a high-risk malignancy...
August 2011: Clinical Journal of Gastroenterology
Fanlong Liu, Pingjiang Ye, Tienan Bi, Lisong Teng, Charlie Xiang, Haohao Wang, Yifei Li, Ketao Jin, Xiaozhou Mou
BACKGROUND/AIMS: Increased expression of polymeric immunoglobulin receptor (pIgR) in tumor tissue has been detected in various cancer forms. However, the clinical relevance of pIgR in colon cancer hepatic metastasis remains uncertain. The aim of this study was to assess the prognostic value of pIgR in patients with colon carcinoma hepatic metastasis after hepatic resection. METHODOLOGY: Genome-wide gene expression analysis was used to evaluate the expression of pIgR in cryopreserved tissue from liver metastases of colon cancer and in the corresponding primary colon cancer tissues from one patient with hepatic metastatic colon cancer...
May 2014: Hepato-gastroenterology
Melanie Johncilla, Joseph Misdraji, Daniel S Pratt, Agoston T Agoston, Gregory Y Lauwers, Amitabh Srivastava, Leona A Doyle
Ipilimumab is a monoclonal antibody that inhibits the CTLA4 receptor on cytotoxic T lymphocytes, resulting in immune-mediated tumor cell death. Ipilimumab is most often used in the treatment of metastatic melanoma, and rarely liver toxicity necessitating cessation of treatment occurs. The aim of this study was to characterize the histologic features and clinical course of ipilimumab-associated hepatitis. Eleven patients with clinical suspicion of ipilimumab-induced hepatitis, due to the development of abnormal liver function tests (LFTs) while receiving treatment, and who underwent liver biopsy, were identified over a 6-year period...
August 2015: American Journal of Surgical Pathology
Yong Li, Da-Jiang Li, Jian Chen, Wei Liu, Jian-Wei Li, Peng Jiang, Xin Zhao, Fei Guo, Xiao-Wu Li, Shu-Guang Wang
OBJECTIVE: To explore the expression of lamininγ2 in extrahepatic cholangiocarcinoma (EHCC) tissues and its influence on tumor invasion and metastasis. MATERIALS AND METHODS: Paraffin embedding samples of cancer, para-cancer, lymph node metastatic and hepatic metastatic tissues from 79 patients undergoing EHCC resection were collected. Expression of lamininγ2 was detected by immunohistochemistry and its relationship with clinical pathological characteristics and the prognosis of EHCC patients were analyzed...
2015: Asian Pacific Journal of Cancer Prevention: APJCP
Y H Shen, S Chen, W T Zhang, Y Ji, L Yu, H C Sun, S J Qiu, N Ren, J Zhou
OBJECTIVE: The objective was to study the clinicopathologic features, grading, treatment protocols, and prognostic of gastroenteropancreatic neuroendocrine tumor (NET) with liver metastasis and primary hepatic NET. MATERIALS AND METHODS: The clinical data of 34 patients with hepatic NET were retrospectively reviewed. According to the primary tumor location and 2010 World Health Organization classification, the cases were categorized to analyze the clinicopathologic features, treatment condition, and prognostic factors...
December 2014: Journal of Cancer Research and Therapeutics
Li Tao, Jin-Kun Yang, Ying Gu, Xin Zhou, Ai-Guang Zhao, Jian Zheng, Ying-Jie Zhu
AIM: To examine the effect of Weichang'an (WCA) and 5-fluorouracil (5-FU) on colorectal tumor and hepatic metastasis in a mouse model. METHODS: Quantitative determination of hesperidin, the effective component in WCA decoction, was performed using HPLC. In vitro cytotoxicity of WCA was determined by treating HCT-116 cells with WCA diluents or serum extracted from rats that received WCA by oral gavage for 1 wk and MTT assays. Forty-eight nude mice received cecum implantation with tumor blocks subcutaneously amplified from human colon cancer cell line HCT-116...
January 28, 2015: World Journal of Gastroenterology: WJG
Stephen M Lagana, Marcela Salomao, Helen E Remotti, A S Knisely, Roger K Moreira
AIMS: Bile salt export pump (BSEP) is a transporter expressed exclusively at hepatic canaliculi and drives bile-salt efflux. Minimal data exist about BSEP expression in tumours. We hypothesized that BSEP immunohistochemistry would be specific for hepatocellular carcinoma (HCC). METHODS AND RESULTS: Tissue microarrays, including 48 HCC, 41 cholangiocarcinomas and 24 metastatic tumours in liver, were immunostained for BSEP. Expression was compared with common markers of hepatocytic differentiation including CD10, hepatocyte paraffin-1 antigen (HepPar-1), carcinoembryonic antigen, arginase-1 (ARG) and glypican-3 (GPC-3)...
March 2015: Histopathology
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