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Immunohistochemistry for carcinoma of unknown primary

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https://www.readbyqxmd.com/read/28634564/pax8-distinguishes-diffuse-large-b-cell-lymphoma-mimicking-sarcoma
#1
Michelle S Hirsch, Alessandra F Nascimento
PAX8 is important for embryogenesis of the thyroid, Müllerian system, and upper urinary/renal tract, and expression of PAX8 has been described in carcinomas from each of these sites. The sensitivity and specificity of the polyclonal PAX8 antibody in a large cohort of epithelial tumors as well as lymphomas have been previously determined, the latter because polyclonal PAX8 is known to be immunoreactive in nonneoplastic B-cell lymphocytes which are often used as the positive internal control for immunohistochemistry...
2017: Case Reports in Pathology
https://www.readbyqxmd.com/read/28552827/metastatic-thyroid-carcinoma-without-identifiable-primary-tumor-within-the-thyroid-gland-a-retrospective-study-of-a-rare-phenomenon
#2
Bin Xu, Theresa Scognamiglio, Perry R Cohen, Manju L Prasad, Adnan Hasanovic, R Michael Tuttle, Nora Katabi, Ronald A Ghossein
Metastatic papillary thyroid carcinoma without an identifiable primary tumor despite extensive microscopic examination of the thyroid gland is a rare but true phenomenon. We retrieved seven of such cases and described in details the clinical and pathologic features of these tumors. BRAF V600E immunohistochemistry and Sequenom molecular profile were conducted in selected cases. All patients harbored metastatic disease in the central (n=3), lateral (n=3), or both neck compartments (n=1). The histotype of the metastatic disease was papillary thyroid carcinoma (PTC, n=5), poorly differentiated thyroid carcinoma (PDTC) in association with a PTC columnar variant (n=1), and anaplastic thyroid carcinoma (ATC) in association with a PTC tall cell variant (n=1)...
May 25, 2017: Human Pathology
https://www.readbyqxmd.com/read/28457039/carcinoma-of-unknown-primary-presenting-as-peripheral-symmetric-gangrene
#3
Abhenil Mittal, Sanchit Sharma, Vineet Govinda Gupta, R Ragesh, Neeraj Nischal, Ajay Gogia, Manish Soneja, Chandan J Das, S K Sharma
Cancer of unknown primary accounts for almost 4-5% of all invasive cancers and consists of tumors from various primary sources with considerable heterogeneity in biology and behaviour. Most of these tumors present with symptoms due to distant metastasis. Histology, immunohistochemistry and molecular profiling is the mainstay for diagnosis. In most cases of adenocarinoma associated with paraneoplastic acral vascular syndrome (PAVS), a site in the lung, ovary or uterus is discernible. Here we report a case of metastatic adenoarcinoma of unknown primary presenting as PAVS, a case which to the best of our knowledge has not been reported in published literature...
February 2017: Journal of the Association of Physicians of India
https://www.readbyqxmd.com/read/28454329/overexpression-of-heat-shock-factor-1-is-associated-with-a-poor-prognosis-in-esophageal-squamous-cell-carcinoma
#4
Yukiko Tsukao, Makoto Yamasaki, Yasuhiro Miyazaki, Tomoki Makino, Tsuyoshi Takahashi, Yukinori Kurokawa, Hiroshi Miyata, Kiyokazu Nakajima, Shuji Takiguchi, Koshi Mimori, Masaki Mori, Yuichiro Doki
Heat-shock factor 1 (HSF1) is the primary regulator of the response to various stressors. A previous study showed that HSF1 expression is associated with a poor prognosis in breast cancer and hepatocellular carcinoma; however, the prognostic significance of HSF1 in esophageal squamous cell carcinoma (ESCC) is unknown. Therefore, the present study investigated the association between HSF1 expression and the clinicopathological parameters of patients, as well as the association between HSF1 expression, and heat shock protein (Hsp)27, Hsp70 and Hsp90 expression induced by HSF1, by cDNA microarray and immunohistochemistry analyses...
March 2017: Oncology Letters
https://www.readbyqxmd.com/read/28440661/morphological-features-and-prognostic-significance-of-arid1a-deficient-esophageal-adenocarcinomas
#5
Michael G Drage, Mingkhwan Tippayawong, Agoston T Agoston, Yifan Zheng, Raphael Bueno, Jason L Hornick, Robert D Odze, Amitabh Srivastava
CONTEXT: - The clinicopathologic and prognostic significance of ARID1A mutation in esophageal adenocarcinoma (EAC) is unknown. OBJECTIVE: - To determine the morphological correlates and prognostic significance of ARID1A-deficient EAC. DESIGN: - One hundred twenty cases of primary EAC were evaluated for a predetermined set of histologic features and immunohistochemistry for ARID1A, p53, and MLH1 performed on EAC, as well as adjacent Barrett esophagus and Barrett esophagus-associated dysplasia, when feasible...
April 25, 2017: Archives of Pathology & Laboratory Medicine
https://www.readbyqxmd.com/read/28423684/prognostic-clinicopathologic-factors-in-carcinoma-of-unknown-primary-origin-a-study-of-106-consecutive-cases
#6
Junjeong Choi, Ji Hae Nahm, Sang Kyum Kim
A heterogeneous group of cancers for which the site of origin remains occult after detailed investigations is defined as carcinomas of unknown primary origin (CUPs). Because patients with CUP have a dismal prognosis, we have analyzed CUPs to highlight the implication of clinicopathologic factors related with patient survival. A total of 106 consecutive cases of CUP were collected. A two-step strategy of immunohistochemistry to assess CUPs according NCCN Guidelines is used to separate carcinomatous tumors and subtype carcinomas...
March 8, 2017: Oncotarget
https://www.readbyqxmd.com/read/28421464/a-novel-tp53-mutation-associated-with-twist1-and-sip1-expression-in-an-aggressive-adrenocortical-carcinoma
#7
Daniel Bulzico, Davi Coe Torres, Gerson Moura Ferreira, Bruno Ricardo Barreto Pires, Paulo Antônio Silvestre de Faria, Rocio Hassan, Eliana Abdelhay, Mario Vaisman, Leonardo Vieira Neto
Adrenocortical carcinomas (ACC) are very rare tumors related to TP53 mutations mostly in childhood onset cases. Epithelial-mesenchymal transition (EMT) transcription factors TWIST1 and Smad interacting protein 1 (SIP1) are related to poorer outcomes in other malignancies, but their role in ACC is unknown. We describe a case of an advanced metastatic ACC (Weiss-score of 9) in a patient at age 76. After primary tumor resection, mitotane therapy was started as palliation to low-volume liver metastasis. After a 2-year period of stable disease, the patient died due to brain metastasis...
April 18, 2017: Endocrine Pathology
https://www.readbyqxmd.com/read/28415703/bromodomain-protein-4-is-a-novel-predictor-of-survival-for-gastric-carcinoma
#8
Yixin Zhu, Weijin Yang, Guangnian Ji, Nan Lin, Weihang Wu, Ping Xiong, Chenxin Zheng, Lei Yan, Peng Wan, Yu Wang
Expression of bromodomain protein 4 (BRD4) has been reported to predict a worse prognosis in solid tumors. However, its expression profile and prognostic value in gastric carcinoma (GC) remains unknown. Here we investigated BRD4 expression in GC and explored its association with patient survival. Tissue samples were obtained from 95 GC patients who underwent surgical resection to remove the primary tumor from January 2009 to December 2010. Immunohistochemistry was used to detect the expression of BRD4 in GC tissues and adjacent normal tissues...
May 9, 2017: Oncotarget
https://www.readbyqxmd.com/read/28391557/cutaneous-basal-cell-carcinoma-with-distant-metastasis-to-thorax-and-bone-a-clinicopathological-and-immunohistochemical-study-of-15-cases
#9
Annikka Weissferdt, Neda Kalhor, Cesar A Moran
Basal cell carcinoma (BCC) is the most common cutaneous malignancy and is characterized by a high cure rate after local excision. Metastatic BCC (mBCC) is an exceedingly rare event, associated with poor prognosis. We report clinicopathological and immunohistochemical features of 15 mBCC metastatic to the thorax and bone. The patients were 14 males and one female with a mean age at diagnosis of cutaneous BCC of 53 years and at metastasis of 64 years. Primary BCC were primarily located in the head and neck or torso and microscopically included five nodular, three basosquamous, three mixed, and one each of superficial, infiltrative, micronodular, and morpheaform types...
June 2017: Virchows Archiv: An International Journal of Pathology
https://www.readbyqxmd.com/read/28336434/vav1-expression-is-increased-in-esophageal-squamous-cell-carcinoma-and-indicates-poor-prognosis
#10
Xiaolei Zhu, Huan Jin, Ziwei Xia, Xianxian Wu, Mingjian Yang, Hongdian Zhang, Xiaobin Shang, Runfen Cheng, Zhongli Zhan, Zhentao Yu
Recently, Vav1 has been suggested to play an essential role in the progression of human cancers. However, the correlation between Vav1 expression and prognosis of esophageal squamous cell carcinoma (ESCC) is still unknown. The aim of this study was to investigate Vav1 expression and its prognostic value in ESCC. The expression of Vav1 was detected by real-time quantitative reverse transcription polymerase chain reaction (qRT-PCR) and Western blotting in ESCC tissues and matched nontumorous tissues. Immunohistochemistry (IHC) was carried out to detect Vav1 expression in paraffin samples from 112 primary ESCC patients...
April 29, 2017: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/28025080/expression-of-mtorc1-2-related-proteins-in-primary-and-brain-metastatic-lung-adenocarcinoma
#11
MULTICENTER STUDY
Ildikó Krencz, Anna Sebestyén, Katalin Fábián, Ágnes Márk, Judit Moldvay, András Khoor, László Kopper, Judit Pápay
Brain metastases (BMs) are common complications of adenocarcinomas (ADCs) of the lung and are associated with a poor prognosis. Although an increasing amount of data indicates that dysregulated activity of mammalian target of rapamycin (mTOR) can influence the metastatic potential of various tumors, the role of mTOR complexes in the development of BMs from ADCs of the lung is largely unknown. To estimate mTOR activity, we studied the expression of mTOR-related proteins (mTORC1: p-mTOR, p-S6; mTORC2: p-mTOR, Rictor) in primary (n=67) and brain metastatic (n=67) lung ADCs, including 15 paired tissue samples, using immunohistochemistry and tissue microarrays...
April 2017: Human Pathology
https://www.readbyqxmd.com/read/27927654/expression-of-sodium-dependent-dicarboxylate-transporter-1-nadc1-slc13a2-in-normal-and-neoplastic-human-kidney
#12
Hyun-Wook Lee, Mary E Handlogten, Gunars Osis, William L Clapp, Dara N Wakefield, Jill W Verlander, I David Weiner
Regulated dicarboxylate transport is critical for acid-base homeostasis, prevention of calcium nephrolithiasis, regulation of collecting duct sodium chloride transport, and the regulation of blood pressure. Although luminal dicarboxylate reabsorption via NaDC1 (SLC13A2) is believed to be the primary mechanism regulating renal dicarboxylate transport, the specific localization of NaDC1 in the human kidney is currently unknown. This study's purpose was to determine NaDC1's expression in normal and neoplastic human kidneys...
March 1, 2017: American Journal of Physiology. Renal Physiology
https://www.readbyqxmd.com/read/27894751/polymerase-%C3%AE%C2%B5-pole-ultra-mutation-in-uterine-tumors-correlates-with-t-lymphocyte-infiltration-and-increased-resistance-to-platinum-based-chemotherapy-in-vitro
#13
Stefania Bellone, Eliana Bignotti, Silvia Lonardi, Francesca Ferrari, Floriana Centritto, Alice Masserdotti, Francesca Pettinella, Jonathan Black, Gulden Menderes, Gary Altwerger, Pei Hui, Salvatore Lopez, Christopher de Haydu, Elena Bonazzoli, Federica Predolini, Luca Zammataro, Emiliano Cocco, Federico Ferrari, Antonella Ravaggi, Chiara Romani, Fabio Facchetti, Enrico Sartori, Franco E Odicino, Dan-Arin Silasi, Babak Litkouhi, Elena Ratner, Masoud Azodi, Peter E Schwartz, Alessandro D Santin
OBJECTIVE: Up to 12% of all endometrial-carcinomas (EC) harbor DNA-polymerase-ε-(POLE) mutations. It is currently unknown whether the favorable prognosis of POLE-mutated EC is derived from their low metastatic capability, extraordinary number of somatic mutations thus imparting immunogenicity, or a high sensitivity to chemotherapy. METHODS: Polymerase-chain-reaction-amplification and Sanger-sequencing were used to test for POLE exonuclease-domain-mutations (exons 9-14) 131 EC...
January 2017: Gynecologic Oncology
https://www.readbyqxmd.com/read/27759689/complete-spontaneous-regression-of-merkel-cell-carcinoma-after-biopsy-a-case-report-and-review-of-the-literature
#14
REVIEW
Parnian Ahmadi Moghaddam, Kristine M Cornejo, Lloyd Hutchinson, Keith Tomaszewicz, Karen Dresser, April Deng, Patrick OʼDonnell
Merkel cell carcinoma (MCC) is a rare primary cutaneous neuroendocrine tumor that typically occurs on the head and neck of the elderly and follows an aggressive clinical course. Merkel cell polyomavirus (MCPyV) has been identified in up to 80% of cases and has been shown to participate in MCC tumorigenesis. Complete spontaneous regression of MCC has been rarely reported in the literature. We describe a case of a 79-year-old man that presented with a rapidly growing, 3-cm mass on the left jaw. An incisional biopsy revealed MCC...
November 2016: American Journal of Dermatopathology
https://www.readbyqxmd.com/read/27729431/prognostic-relevance-of-epithelial-mesenchymal-transition-and-proliferation-in-surgically-treated-primary-parotid-gland-cancer
#15
Alina Busch, Larissa Bauer, Eva Wardelmann, Claudia Rudack, Inga Grünewald, Markus Stenner
AIMS: Cancer of the major salivary glands comprises a morphologically diverse group of rare tumours of largely unknown cause. Epithelial-mesenchymal transition (EMT) has been shown to play a significant prognostic role in various human cancers. The aim was to assess the expression of EMT markers in different histological subtypes of parotid gland cancer (PGC) and analyse their prognostic value. METHODS: We examined 94 PGC samples (13 histological subtypes) for the expression of MIB-1, epithelial cadherin (E-cadherin), β-catenin, vimentin and cytokeratin 8/18 (CK8/18) by means of immunohistochemistry...
May 2017: Journal of Clinical Pathology
https://www.readbyqxmd.com/read/27713405/molecular-analysis-of-aggressive-renal-cell-carcinoma-with-unclassified-histology-reveals-distinct-subsets
#16
Ying-Bei Chen, Jianing Xu, Anders Jacobsen Skanderup, Yiyu Dong, A Rose Brannon, Lu Wang, Helen H Won, Patricia I Wang, Gouri J Nanjangud, Achim A Jungbluth, Wei Li, Virginia Ojeda, A Ari Hakimi, Martin H Voss, Nikolaus Schultz, Robert J Motzer, Paul Russo, Emily H Cheng, Filippo G Giancotti, William Lee, Michael F Berger, Satish K Tickoo, Victor E Reuter, James J Hsieh
Renal cell carcinomas with unclassified histology (uRCC) constitute a significant portion of aggressive non-clear cell renal cell carcinomas that have no standard therapy. The oncogenic drivers in these tumours are unknown. Here we perform a molecular analysis of 62 high-grade primary uRCC, incorporating targeted cancer gene sequencing, RNA sequencing, single-nucleotide polymorphism array, fluorescence in situ hybridization, immunohistochemistry and cell-based assays. We identify recurrent somatic mutations in 29 genes, including NF2 (18%), SETD2 (18%), BAP1 (13%), KMT2C (10%) and MTOR (8%)...
October 7, 2016: Nature Communications
https://www.readbyqxmd.com/read/27663540/p16-immunohistochemistry-is-a-useful-diagnostic-adjunct-in-cases-of-metastatic-cervical-carcinoma-of-unknown-origin
#17
Marc R Rohrbach, Christopher J Britt, Michael Schwalbe, Aaron M Wieland, Gregory K Hartig
PURPOSE: Metastatic cervical carcinoma of unknown primary (MCCUP) is increasing in frequency owing in part to rising human papillomavirus (HPV)-driven oropharyngeal carcinoma. Identifying the primary site is valuable, because it is associated with increased survival and decreased morbidity. HPV-positive cervical nodal disease focuses attention on the oropharynx for directed biopsy examinations, including tonsillectomy. When the primary is small, carcinoma might not be apparent by traditional hematoxylin and eosin (H&E) staining alone...
March 2017: Journal of Oral and Maxillofacial Surgery
https://www.readbyqxmd.com/read/27422471/primary-colon-adenosquamous-carcinoma-in-a-patient-with-lynch-syndrome-a-new-histologic-subtype-associated-with-microsatellite-instability
#18
Virginia E Duncan, Shuko Harada, Todd M Stevens
We report a case of a 53-year-old woman who presented with rectal bleeding and a 9.5 cm hemicircumferential ascending colon mass. Histology revealed adenosquamous carcinoma (ASC), a rare subtype comprised of malignant squamous and glandular elements. Immunohistochemistry revealed loss of MLH1/PMS2 expression and retained MSH2/MSH6 expression in squamous and glandular components, indicative of microsatellite instability (MSI). MSI is caused by loss-of-function defects in DNA mismatch repair genes, leading to increased susceptibility to a variety of neoplasms; the role of MSI in colorectal ASC is unknown...
October 2016: International Journal of Surgical Pathology
https://www.readbyqxmd.com/read/27220319/expression-and-clinical-significance-of-wee1-in-colorectal-cancer
#19
Eivind Valen Egeland, Kjersti Flatmark, Jahn M Nesland, Vivi Ann Flørenes, Gunhild M Mælandsmo, Kjetil Boye
Wee1 is a nuclear kinase regulating cell cycle progression, and has emerged as a promising therapeutic target in cancer. Expression of Wee1 has been associated with poor outcome in certain tumor types, but the prognostic impact and clinical significance in colorectal cancer is unknown. The expression of Wee1 was examined by immunohistochemistry in primary colorectal carcinomas from a prospectively collected patient cohort, and associations with clinicopathological parameters and outcome were investigated. Cell culture experiments were performed using the cell lines RKO and SW620, and the relationship with the metastasis-promoting protein S100A4 was investigated...
September 2016: Tumour Biology: the Journal of the International Society for Oncodevelopmental Biology and Medicine
https://www.readbyqxmd.com/read/27196434/association-of-extracapsular-spread-with-survival-according-to-human-papillomavirus-status-in-oropharynx-squamous-cell-carcinoma-and-carcinoma-of-unknown-primary-site
#20
Natallia Kharytaniuk, Peter Molony, Seamus Boyle, Gerard O'Leary, Reiltin Werner, Cynthia Heffron, Linda Feeley, Patrick Sheahan
IMPORTANCE: The presence of extracapsular spread (ECS) of metastatic nodes is considered a poor prognosticator in head and neck cancer, with postoperative chemoradiation therapy often recommended over radiation therapy alone in such cases. However, there is less clarity regarding the effect of ECS on human papillomavirus-associated oropharynx squamous cell carcinoma (OPSCC) or carcinoma of unknown primary site (CUP). OBJECTIVE: To investigate the association of ECS according to human papillomavirus status in OPSCC and CUP with survival...
July 1, 2016: JAMA Otolaryngology—Head & Neck Surgery
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