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https://www.readbyqxmd.com/read/29667261/apple-s-rnase-interacts-with-an-actin-binding-protein-mdmvg-to-reduce-pollen-tube-growth-by-inhibiting-its-actin-severing-activity-at-the-early-stage-of-self-pollination-induction
#1
Qing Yang, Dong Meng, Zhaoyu Gu, Wei Li, Qiuju Chen, Yang Li, Hui Yuan, Jie Yu, Chunsheng Liu, Tianzhong Li
In S-RNase-mediated self-incompatibility (SI), S-RNase secreted from the style destroys the actin cytoskeleton of the self-pollen tubes, eventually halting its growth, but the mechanism remains unclear. In vitro biochemical assays revealed that S-RNase does not bind or sever filamentous actin (F-actin). In apple (Malus×domestica), we identified an actin-binding protein containing myosin, villin and GRAM, MdMVG, that physically interacts with S-RNase and directly binds and severs F-actin. Immunofluorescence assays and total internal reflection fluorescence microscopy (TIRFM) indicated that S-RNase inhibits the F-actin-severing activity of MdMVG in vitro...
April 18, 2018: Plant Journal: for Cell and Molecular Biology
https://www.readbyqxmd.com/read/29667089/sex-skull-length-breed-and-age-predict-how-dogs-look-at-faces-of-humans-and-conspecifics
#2
Zsófia Bognár, Ivaylo B Iotchev, Enikő Kubinyi
The gaze of other dogs and humans is informative for dogs, but it has not been explored which factors predict face-directed attention. We used image presentations of unfamiliar human and dog heads, facing the observer (portrait) or facing away (profile), and measured looking time responses. We expected dog portraits to be aversive, human portraits to attract interest, and tested dogs of different sex, skull length and breed function, which in previous work had predicted human-directed attention. Dog portraits attracted longer looking times than human profiles...
April 17, 2018: Animal Cognition
https://www.readbyqxmd.com/read/29665002/three-dimensional-collagenous-niche-and-azacytidine-selectively-promote-time-dependent-cardiomyogenesis-from-human-bone-marrow-derived-msc-spheroids
#3
Jyotsna Joshi, Gautam Mahajan, Chandrasekhar R Kothapalli
Endogenous adult cardiac regenerative machinery is not capable of replacing the lost cells following myocardial infarction, often leading to permanent alterations in structure-function-mechanical properties. Regenerative therapies based on delivering autologous stem cells within an appropriate 3D milieu could meet such demand, by enabling homing and directed differentiation of the transplanted cells into lost specialized cell populations. Since type I collagen is the predominant cardiac tissue matrix protein, we here optimized the 3D niche which could promote time-dependent evolution of cardiomyogenesis from human bone marrow-derived mesenchymal stem cells (BM-MSC)...
April 17, 2018: Biotechnology and Bioengineering
https://www.readbyqxmd.com/read/29664890/health-literacy-and-quality-of-physician-trauma-patient-communication-opportunity-for-improvement
#4
Jonathan L Dameworth, Jordan A Weinberg, Pamela W Goslar, Dana J Stout, Sharjeel Israr, Jordan V Jacobs, Thomas L Gillespie, Terrell M Thompson, Scott R Petersen
BACKGROUND: Although physician-patient communication and health literacy (HL) have been studied in diverse patient groups, there has been little focus on trauma patients. A quality improvement project was undertaken at our level 1 trauma center to improve patient perception of physician-patient communication, with consideration of the effect of HL. We report the first phase of this project, namely the reference level of satisfaction with physician-patient communication as measured by levels of interpersonal care among patients at an urban level 1 trauma center...
April 16, 2018: Journal of Trauma and Acute Care Surgery
https://www.readbyqxmd.com/read/29664205/selective-and-sensitive-escherichia-coli-detection-based-on-a-t4-bacteriophage-immobilized-multimode-microfiber
#5
Yanpeng Li, Hui Ma, Lin Gan, Andong Gong, Haibin Zhang, Deming Liu, Qizhen Sun
Escherichia coli (E.coli) bacteria have been found to be responsible for various health outbreaks caused by contaminated food and water. Accurate and rapid test of E.coli is thus crucial for protecting the public health. A fast-response, label-free bacteriophage-based detection of E.coli using multimode microfiber probe is proposed and demonstrated in this paper. Due to the abrupt taper and subwavelength diameter, different modes are excited and guided in the microfiber as evanescent field that can interact with surrounding E...
April 17, 2018: Journal of Biophotonics
https://www.readbyqxmd.com/read/29662092/diffusion-limited-growth-of-microbial-colonies
#6
Hayden Tronnolone, Alexander Tam, Zoltán Szenczi, J E F Green, Sanjeeva Balasuriya, Ee Lin Tek, Jennifer M Gardner, Joanna F Sundstrom, Vladimir Jiranek, Stephen G Oliver, Benjamin J Binder
The emergence of diffusion-limited growth (DLG) within a microbial colony on a solid substrate is studied using a combination of mathematical modelling and experiments. Using an agent-based model of the interaction between microbial cells and a diffusing nutrient, it is shown that growth directed towards a nutrient source may be used as an indicator that DLG is influencing the colony morphology. A continuous reaction-diffusion model for microbial growth is employed to identify the parameter regime in which DLG is expected to arise...
April 16, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29661458/ombitasvir-paritaprevir-ritonavir-therapy-in-a-kidney-transplant-recipient-with-chronic-hepatitis-c-virus-genotype-1-infection-a-case-report-on-the-importance-of-considering-drug-drug-interactions-and-monitoring-cyclosporine-levels
#7
S Takeuchi, M Takamura, T Yoshida, K Takahashi, K Hayashi, S Hashimoto, S Yamagiwa, M Tasaki, Y Nakagawa, K Saito, Y Tanabe, Y Tomita, S Terai
A 74-year-old Japanese man with a history of chronic hepatitis C and kidney transplant (KT) was administered pegylated-interferon plus ribavirin therapy. However, this therapy was ineffective. The patient was then hospitalized to receive ombitasvir (OBV) plus paritaprevir (PTV) plus ritonavir (r) antiviral combination therapy. He tested negative for the virus after 4 weeks, and completed 12 weeks of treatment. The patient ultimately achieved a sustained virological response after the 12 weeks of treatment. Cyclosporine (CyA) trough levels, during the OBV-PTV-r therapy, reached a peak within 5 days of initiating therapy, and increases in serum creatinine and total bilirubin were also observed...
April 2018: Transplantation Proceedings
https://www.readbyqxmd.com/read/29659663/real-world-evidence-for-regulatory-decisions-concomitant-administration-of-zoster-vaccine-live-and-pneumococcal-polysaccharide-vaccine
#8
Katia Bruxvoort, Lina S Sy, Yi Luo, Hung Fu Tseng
The US Food and Drug Administration is charged with expanding the use of real world evidence (RWE) for regulatory decisions. As a test case for RWE to support regulatory decisions, we present the scenario of concomitant vaccination with zoster vaccine live (ZVL) and 23-valent pneumococcal polysaccharide vaccine (PPSV23). The prescribing information states that these vaccines should not be given concurrently, based on a small trial using varicella zoster virus antibody levels as a correlate of ZVL efficacy, even though ZVL protects against herpes zoster via cell-mediated immunity...
April 11, 2018: American Journal of Epidemiology
https://www.readbyqxmd.com/read/29659062/coacervation-mediated-combinatorial-synthesis-of-biomatrices-for-stem-cell-culture-and-directed-differentiation
#9
Robert Wieduwild, Richard Wetzel, Dejan Husman, Sophie Bauer, Iman El-Sayed, Sarah Duin, Priyanka Murawala, Alvin Kuriakose Thomas, Manja Wobus, Martin Bornhäuser, Yixin Zhang
Combinatorial screening represents a promising strategy to discover biomaterials for tailored cell culture applications. Although libraries incorporating different biochemical cues have been investigated, few simultaneously recapitulate relevant biochemical, physical, and dynamic features of the extracellular matrix (ECM). Here, a noncovalent system based on liquid-liquid phase separation (coacervation) and gelation mediated by glycosaminoglycan (GAG)-peptide interactions is reported. Multiple biomaterial libraries are generated using combinations of sulfated glycosaminoglycans and poly(ethylene glycol)-conjugated peptides...
April 16, 2018: Advanced Materials
https://www.readbyqxmd.com/read/29657351/habitat-heterogeneity-induces-rapid-changes-in-the-feeding-behaviour-of-generalist-arthropod-predators
#10
Karin Staudacher, Oskar Rennstam Rubbmark, Klaus Birkhofer, Gerard Malsher, Daniela Sint, Mattias Jonsson, Michael Traugott
The "habitat heterogeneity hypothesis" predicts positive effects of structural complexity on species coexistence. Increasing habitat heterogeneity can change the diversity (number of species, abundances) and the functional roles of communities. The latter, however, is not well understood as species and individuals may respond very differently and dynamically to a changing environment.Here, we experimentally test how habitat heterogeneity affects generalist arthropod predators, including epigaeic spiders, carabid and staphylinid beetles, under natural conditions by assessing their diversity and directly measuring their trophic interactions (which provide a proxy for their functional roles)...
March 2018: Functional Ecology
https://www.readbyqxmd.com/read/29655737/interactions-of-green-coffee-bean-phenolics-with-wheat-bread-matrix-in-a-model-of-simulated-in-vitro-digestion
#11
Michał Świeca, Urszula Gawlik-Dziki, Łukasz Sęczyk, Dariusz Dziki, Małgorzata Sikora
Interactions of phenolics from green coffee bean flour (GCS) with the matrix of wheat bread have been studied employing direct (electrophoretic and chromatographic techniques) and indirect tests (nutrient digestibility). According to the chromatograms of digests, the antiradical activity of enriched bread was exhibited by free phenolics. An increase the area of chromatograms and some additional peaks observed for enriched bread may confirm some interactions of proteins with phenolics. The electrophoretic profile of these extracts showed that the band corresponding to a protein with molecular mass of 38 kDA had much higher intensity in enriched bread...
August 30, 2018: Food Chemistry
https://www.readbyqxmd.com/read/29655729/in-vitro-effects-of-rebaudioside-a-stevioside-and-steviol-on-porcine-cytochrome-p450-expression-and-activity
#12
Rebekka Thøgersen, Bjørn Petrat-Melin, Galia Zamaratskaia, Kai Grevsen, Jette Feveile Young, Martin Krøyer Rasmussen
The physiological effects of the Stevia-derived compounds, rebaudioside A, stevioside and steviol have been the focus of several studies due to their use as sweeteners in food. Despite that, little is known about their potential food-drug interactions. In the present study, IPEC-J2 cells and primary hepatocytes were used to investigate the effect of rebaudioside A, stevioside and steviol on cytochrome p450 (CYP) mRNA expression. Moreover, hepatic microsomes were used to investigate direct interactions between the compounds and specific CYP activity...
August 30, 2018: Food Chemistry
https://www.readbyqxmd.com/read/29655575/crmp2-neurofibromin-interface-drives-nf1-related-pain
#13
Aubin Moutal, Li Sun, Xiaofang Yang, Wennan Li, Song Cai, Shizhen Luo, Rajesh Khanna
An understudied symptom of the genetic disorder Neurofibromatosis type 1 (NF1) is chronic idiopathic pain. We used targeted editing of Nf1 in rats to provide direct evidence of a causal relationship between neurofibromin, the protein product of the Nf1 gene, and pain responses. Our study data identified a protein-interaction network with collapsin response meditator protein 2 (CRMP2) as a node and neurofibromin, syntaxin 1A, and the N-type voltage-gated calcium (CaV2.2) channel as interaction edges. Neurofibromin uncouples CRMP2 from syntaxin 1A...
April 12, 2018: Neuroscience
https://www.readbyqxmd.com/read/29653049/the-influence-of-cationic-phosphatidylcholine-derivative-on-monolayer-and-bilayer-artificial-bacterial-membranes
#14
Marzena Mach, Karolina Węder, Katarzyna Hąc-Wydro, Michał Flasiński, Joanna Lewandowska-Lancucka, Kinga Wójcik, Pawel Wydro
An increasing number of bacterial infections and the rise in antibiotic resistance of a number of bacteria speciesforces to search for new antibacterial compounds. The latter facts motivate the investigations presented herein and aimed at studying the influence of a cationic lipid:1-palmitoyl-2-oleoyl-sn-glycero-3-ethylphosphocholine (EPOPC) on model (mono,- and bilayer) membranes. The monolayer experiments involved the analysis of the interactions of EPOPC with bacterial membrane lipids in one component and mixed systems as well as Brewster Angle Microcopy studies...
April 13, 2018: Langmuir: the ACS Journal of Surfaces and Colloids
https://www.readbyqxmd.com/read/29652992/-phenotypic-and-mutational-analysis-of-a-pedigree-affected-with-hereditary-coagulation-factor-%C3%A2-deficiency
#15
Mengcha Tian, Hong Xia, Zhishan Zhang, Yanhui Jin, Kankan Su, Mingshan Wang
OBJECTIVE: To explore the molecular pathogenesis for a pedigree affected with coagulation factor Ⅴ (FⅤ) deficiency. METHODS: Prothrombin time (PT), activated partial thromboplastin time (APTT), fibrinogen (FIB), coagulation factor Ⅱ activity (FⅡ: C), FⅤ activity (FⅤ: C), coagulation factor Ⅶ activity (FⅦ: C), and coagulation factor Ⅹ activity (FⅩ: C) were determined with a STAGO automatic coagulometer. FⅤ antigen (FⅤ: Ag) was detected with enzyme linked immunosorbent assay (ELISA)...
April 10, 2018: Zhonghua Yi Xue Yi Chuan Xue za Zhi, Zhonghua Yixue Yichuanxue Zazhi, Chinese Journal of Medical Genetics
https://www.readbyqxmd.com/read/29652679/comparison-of-static-and-dynamic-balance-at-different-levels-of-sport-competition-in-professional-and-junior-elite-soccer-players
#16
Łukasz Jadczak, Monika Grygorowicz, Witold Dzudziński, Robert Śliwowski
Jadczak, Ł, Grygorowicz, M, Dzudziński, W, and Śliwowski, R. Comparison of static and dynamic balance at different levels of sport competition in professional and junior elite soccer players. J Strength Cond Res XX(X): 000-000, 2018-The purpose of this study was to compare body balance control and balance recovery strategies of professional football players, representing various sports levels in static (eyes open, eyes closed) and dynamic conditions, both on the dominant and nondominant leg. Three groups of professional and junior elite soccer players were investigated: a PRO group (n = 52), a U-21 group (n = 55), and a U-19 group (n = 47)...
April 12, 2018: Journal of Strength and Conditioning Research
https://www.readbyqxmd.com/read/29649338/guilt-leads-to-enhanced-facing-the-viewer-bias
#17
Mowei Shen, Chengfeng Zhu, Huayu Liao, Haihang Zhang, Jifan Zhou, Zaifeng Gao
As an important moral emotion, guilt plays a critical role in social interaction. It has been found that people tended to exhibit prosocial behavior under circumstances of guilt. However, all extant studies have predominantly focused on the influence of guilt on macro-level behavior. So far, no study has investigated whether guilt affects people's micro-level perception. The current study closes this gap by examining whether guilt affects one's inclination to perceive approaching motion. We achieved this aim by probing a facing-the-viewer bias (FTV bias)...
2018: PloS One
https://www.readbyqxmd.com/read/29645318/investigating-the-dynamics-and-polyanion-binding-sites-of-fibroblast-growth-factor-1-using-hydrogen-deuterium-exchange-mass-spectrometry
#18
Siva K Angalakurthi, Connie A Tenorio, Michael Blaber, C Russell Middaugh
In this study, we examined the local dynamics of acidic fibroblast growth factor (FGF-1) as well as the binding sites of various polyanions including poly-sulfates (heparin and low MW heparin) and poly-phosphates (phytic acid and ATP) using hydrogen-deuterium exchange mass spectrometry (HX-MS). For local dynamics, results are analyzed at the peptide level as well as in terms of buried amides employing crystallographic B-factors and compared with a residue level heat map generated from HX-MS results. Results show that strand 4 and 5 and the turn between them to be the most flexible regions as was previously seen by NMR...
April 12, 2018: Protein Science: a Publication of the Protein Society
https://www.readbyqxmd.com/read/29643235/developing-and-evaluating-inhibitors-against-the-rnase-h-active-site-of-hiv-1-rt
#19
Paul L Boyer, Steven J Smith, Xue Zhi Zhao, Kalyan Das, Kevin Gruber, Eddy Arnold, Terrence R Burke, Stephen H Hughes
We tested three compounds for their ability to inhibit the RNase H (RH) and polymerase activities of HIV-1 reverse transcriptase (RT). A high-resolution crystal structure (2.2 Å) of one of the compounds showed that it chelates the two magnesium ions at the RH active site; this prevents the RH active site from interacting with, and cleaving, the RNA strand of an RNA/DNA heteroduplex. The compounds were tested using a variety of substrates: All three compounds inhibited the polymerase-independent RH activity of HIV-1 RT...
April 11, 2018: Journal of Virology
https://www.readbyqxmd.com/read/29642500/antibacterial-free-fatty-acids-and-monoglycerides-biological-activities-experimental-testing-and-therapeutic-applications
#20
REVIEW
Bo Kyeong Yoon, Joshua A Jackman, Elba R Valle-González, Nam-Joon Cho
Antimicrobial lipids such as fatty acids and monoglycerides are promising antibacterial agents that destabilize bacterial cell membranes, causing a wide range of direct and indirect inhibitory effects. The goal of this review is to introduce the latest experimental approaches for characterizing how antimicrobial lipids destabilize phospholipid membranes within the broader scope of introducing current knowledge about the biological activities of antimicrobial lipids, testing strategies, and applications for treating bacterial infections...
April 8, 2018: International Journal of Molecular Sciences
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