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https://www.readbyqxmd.com/read/29767241/transcriptome-sequencing-analysis-reveals-the-effect-of-combinative-treatment-with-low%C3%A2-intensity-pulsed-ultrasound-and-magnesium-ions-on-hfob1-19-human-osteoblast-cells
#1
Haiyue Zu, Xueting Yi, Dewei Zhao
Biodegradable magnesium (Mg) materials are considered ideal as osteosynthesis implants. However, clinical application has proven complex. This is primarily associated with the issue of reducing the extent of implant degradation to a range acceptable for the human body, while simultaneously enhancing osteogenesis or osteoinduction. In the present study, a combination of Mg ions and low‑intensity pulsed ultrasound (LIPUS) treatment was applied in hFOB 1.19 human osteoblast cells as a potential strategy to resolve this issue...
May 11, 2018: Molecular Medicine Reports
https://www.readbyqxmd.com/read/29689491/a-single-integrated-osteochondral-in-situ-composite-scaffold-with-a-multi-layered-functional-structure
#2
Taijun Chen, Jiafan Bai, Jiajun Tian, Pinhe Huang, Hua Zheng, Jianxin Wang
This work focuses on the optimization design of a functional biomimetic scaffold for the repair of osteochondral defects and includes the study of single integrated osteochondral tissue engineering scaffolds with a multi-layered functional structure. Rabbit model experiments were used to evaluate the repair of osteochondral defects. The results revealed that good integration was achieved both at the interfaces between the scaffold material and the host tissue and between the newly formed subchondral bone and cartilage...
April 16, 2018: Colloids and Surfaces. B, Biointerfaces
https://www.readbyqxmd.com/read/29688457/microbial-communities-in-bakken-region-produced-water
#3
Daniel Lipus, Dhritikshama Roy, Eakalak Khan, Daniel Ross, Amit Vikram, Djuna Gulliver, Richard Hammack, Kyle Bibby
The Bakken Shale has become one of the United States' most important oil and gas producing regions. This study examined the microbiology and geochemical characteristics of Bakken region produced water from 17 well sites sampled from the three-phase separator and produced water holding tank over a six-month time frame. Produced water samples had high total dissolved solids (TDS) (220,000 mg/L - 350,000 mg/L) and low dissolved organic carbon (DOC) concentrations (41 mg/L - 132 mg/L). Microbial abundances varied between 101-104 16S rRNA gene copies/mL, approximately four orders of magnitude below those observed for produced waters from other hydraulic fracturing regions...
April 23, 2018: FEMS Microbiology Letters
https://www.readbyqxmd.com/read/29664414/enhanced-total-neurite-outgrowth-and-secondary-branching-in-dorsal-root-ganglion-neurons-elicited-by-low-intensity-pulsed-ultrasound
#4
Daniel Michael Ventre, Marissa Puzan, Emily Ashbolt, Abigail Koppes
OBJECTIVE: Despite the prevalence of peripheral nerve injuries (PNI), challenges remain in restoring full functionality to those afflicted. For recovery to occur, axons must extend across the injury site to connect with distal targets, where injury gap size is a critical factor in the probability of restoration of function. Current clinical therapies often achieve limited neural regeneration, motivating the development of new therapeutic interventions such as biophysical stimulation. APPROACH: To investigate the potential for low intensity, pulsed ultrasonic simulation (LIPUS) to impact peripheral nerve regeneration, primary neonatal rat dorsal root ganglion neurons were examined in vitro in response to ultrasound (US)...
April 17, 2018: Journal of Neural Engineering
https://www.readbyqxmd.com/read/29658096/effect-of-low-level-laser-and-low-intensity-pulsed-ultrasound-therapy-on-bone-remodeling-during-orthodontic-tooth-movement-in-rats
#5
Mohammed Mahmood Jawad Alazzawi, Adam Husein, Mohammad Khursheed Alam, Rozita Hassan, Rumaizi Shaari, Ahmad Azlina, M S Salzihan
BACKGROUND: Quality bone regeneration, which leads to the improvement of bone remodeling, is essential for orthodontic treatment. In order to improve bone regeneration and increase the amount of tooth movement, different techniques have been implemented. The object of this study is to compare the effects of low-level laser therapy (LLLT), low-intensity pulsed ultrasound (LIPUS), and their combination on bone remodeling during orthodontic tooth movement. METHODS: Eighty (80) male, 6-week-old Sprague Dawley rats were grouped in to four groups, the first group was irradiated with (940 nm) diode laser, second group with LIPUS, and third group with combination of both LLLT and LIPUS...
April 16, 2018: Progress in Orthodontics
https://www.readbyqxmd.com/read/29620151/low%C3%A2-intensity-pulsed-ultrasound-promotes-periodontal-ligament-stem-cell-migration-through-twist1%C3%A2-mediated-sdf%C3%A2-1-expression
#6
Yunji Wang, Jie Li, Ye Qiu, Bo Hu, Jin Chen, Tiwei Fu, Pengfei Zhou, Jinlin Song
Low‑intensity pulsed ultrasound (LIPUS) is a non‑invasive therapeutic treatment for accelerating fracture healing. A previous study from our group demonstrated that LIPUS has the potential to promote periodontal tissue regeneration. However, the underlying molecular mechanism by which LIPUS promotes periodontal tissue regeneration remains unknown. In the present study, periodontal ligament stem cells (PDLSCs) were isolated from premolars. Flow cytometry and differentiation assays were used to characterize the isolated PDLSCs...
March 27, 2018: International Journal of Molecular Medicine
https://www.readbyqxmd.com/read/29615782/preventive-effect-of-low-intensity-pulsed-ultrasound-against-experimental-cerebral-ischemia-reperfusion-injury-via-apoptosis-reduction-and-brain-derived-neurotrophic-factor-induction
#7
Chang-Mu Chen, Cheng-Tien Wu, Ting-Hua Yang, Shing-Hwa Liu, Feng-Yi Yang
Stroke is known as the top 10 causes of death worldwide. Development of effectively neuroprotective or preventive strategies for ischemia stroke is imperative. For the purpose of stroke prevention, we tested the neuroprotective effects of low-intensity pulsed ultrasound (LIPUS) on ischemic stroke. Adult C57BL/6 mice were used to daily treatment with LIPUS for 5 days on left hemisphere before middle cerebral artery occlusion (MCAO)-induced cerebral ischemia/reperfusion injury. Western blotting and immunohistochemistry were performed to assess the protein expressions of signaling molecules...
April 3, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29573888/effects-of-low-intensity-pulsed-ultrasound-for-preventing-joint-stiffness-in-immobilized-knee-model-in-rats
#8
Nobuyuki Itaya, Yutake Yabe, Yoshihiro Hagiwara, Kenji Kanazawa, Masashi Koide, Takuya Sekiguchi, Shinichirou Yoshida, Yasuhito Sogi, Toshihisa Yano, Masahiro Tsuchiya, Yoshihumi Saijo, Eiji Itoi
The purpose of this study was to examine the effect of low-intensity pulsed ultrasound (LIPUS) in preventing joint stiffness. Unilateral knee joints were immobilized in two groups of rats (n = 6/period/group). Under general anesthesia, the immobilized knee joints were exposed to LIPUS for 20 min/d, 5 d/wk, using an existing LIPUS device (LIPUS group, 1.5-MHz frequency, 1.0-kHz repetition cycle, 200-µs burst width and 30-mW/cm2 power output) until endpoints (2, 4 or 6 wk). In the control group, general anesthesia alone was administered in the same manner as in the other group...
March 21, 2018: Ultrasound in Medicine & Biology
https://www.readbyqxmd.com/read/29542397/-it-is-not-natural-anymore-nutrition-urbanization-and-indigenous-identity-on-bolivia-s-andean-plateau
#9
Adam C Lipus, Juan S Leon, Susana C Calle, Karen L Andes
The objective of this article was to characterize how urbanization and indigenous identity shape nutrition attitudes and practices in El Alto, a rapidly urbanizing and predominantly indigenous (Aymara) community on Bolivia's Andean plateau. We took a qualitative ethnographic approach, interviewing health care providers ( n = 11) and conducting focus groups with mothers of young children ( n = 4 focus groups with 25 mothers total [age = 18-43 years, 60% Aymara]). Participants generally described their urban environment as being problematic for nutrition, a place where unhealthy "junk foods" and "chemicals" have supplanted healthy, "natural," "indigenous" foods from the countryside...
March 1, 2018: Qualitative Health Research
https://www.readbyqxmd.com/read/29471078/development-of-controlled-drug-delivery-systems-for-bone-fracture-targeted-therapeutic-delivery-a-review
#10
REVIEW
Yuchen Wang, Maureen R Newman, Danielle S W Benoit
Impaired fracture healing is a major clinical problem that can lead to patient disability, prolonged hospitalization, and significant financial burden. Although the majority of fractures heal using standard clinical practices, approximately 10% suffer from delayed unions or non-unions. A wide range of factors contribute to the risk for nonunions including internal factors, such as patient age, gender, and comorbidities, and external factors, such as the location and extent of injury. Current clinical approaches to treat nonunions include bone grafts and low-intensity pulsed ultrasound (LIPUS), which realizes clinical success only to select patients due to limitations including donor morbidities (grafts) and necessity of fracture reduction (LIPUS), respectively...
June 2018: European Journal of Pharmaceutics and Biopharmaceutics
https://www.readbyqxmd.com/read/29466082/determination-of-optimum-operation-parameters-for-low-intensity-pulsed-ultrasound-and-low-level-laser-based-treatment-to-induce-proliferation-of-osteoblast-and-fibroblast-cells
#11
Mehmet Emre Coskun, Kubra Acikalin Coskun, Yusuf Tutar
OBJECTIVE: The aim of this study was to determine the optimum operating parameters (pulse duration, energy levels, and application time) to promote induction of osteoblast and fibroblast cell proliferation and to maintain cell viability treated with low-intensity pulsed ultrasound (LIPUS) and low-level laser therapy (LLLT). BACKGROUND DATA: The positive effects of LIPUS and LLLT on cellular activity have been reported in recent years. Comparisons between experimental parameters of previous studies are difficult because scientific studies reported frequencies and the duty cycles of LIPUS and wavelengths and doses of LLLT in a wide range of parameters...
May 2018: Photomedicine and Laser Surgery
https://www.readbyqxmd.com/read/29462805/low-intensity-pulsed-ultrasound-prevents-the-oxidative-stress-induced-endothelial-mesenchymal-transition-in-human-aortic-endothelial-cells
#12
Jiamin Li, Qingwei Zhang, Cong Ren, Xianxian Wu, Ying Zhang, Xue Bai, Yuan Lin, Mingqi Li, Jiangbo Fu, Philipp Kopylov, Siqi Wang, Tingting Yu, Ning Wang, Chaoqian Xu, Yong Zhang, Baofeng Yang
BACKGROUND/AIMS: Endothelial-mesenchymal transition (EndMT) has been shown to take part in the generation and progression of diverse diseases, involving a series of changes leading to a loss of their endothelial characteristics and an acquirement of properties typical of mesenchymal cells. Low-intensity pulsed ultrasound (LIPUS) is a new therapeutic option that has been successfully used in fracture healing. However, whether LIPUS can inhibit oxidative stress-induced endothelial cell damages through inhibiting EndMT remained unknown...
2018: Cellular Physiology and Biochemistry
https://www.readbyqxmd.com/read/29436706/low-intensity-pulsed-ultrasound-increases-blood-vessel-size-during-fracture-healing-in-patients-with-a-delayed-union-of-the-osteotomized-fibula
#13
Clara M Korstjens, Sjoerd Rutten, Peter A Nolte, Marion A van Duin, Jenneke Klein-Nulend
Disturbed vascularity leads to impaired fracture healing. Since low-intensity pulsed ultrasound (LIPUS) increases new bone formation in delayed-unions, we investigated whether LIPUS increases blood supply in delayed-unions of the osteotomized fibula, and if LIPUS-increased bone formation is correlated to increased blood supply. Blood vessel parameters were analysed using histology, immunohistochemistry, and histomorphometric analysis as well as their correlation with bone formation and resorption parameters...
February 13, 2018: Histology and Histopathology
https://www.readbyqxmd.com/read/29422994/effect-of-low-intensity-pulsed-ultrasound-on-the-biological-behaviors-of-bone-marrow-mesenchymal-stem-cells-on-titanium-with-different-surface-topographies
#14
Yanxin An, Yan Song, Zhaoling Wang, Jing Wang, Gaoyi Wu, Guoxiong Zhu, Lei Chen
The use of low-intensity pulsed ultrasound (LIPUS) is a promising approach to promote osteogenesis. However, few studies have reported the influence of this technique on the osseointegration of endosseous implants, especially regarding different implant topographies. We focused on how the initial interaction between cells and the titanium surface is enhanced by LIPUS and the potential regulatory mechanisms. The bone marrow mesenchymal stem cells (BMSCs) of rats were cultured on two types of titanium surfaces (polished surface, Flat and large grain blast acid etched, SLA) under LIPUS stimulation or control conditions...
2018: American Journal of Translational Research
https://www.readbyqxmd.com/read/29414599/low-intensity-pulsed-ultrasound-promotes-spinal-fusion-and-enhances-migration-and-proliferation-of-mg63s-through-sonic-hedgehog-signaling-pathway
#15
Xiao-Yi Zhou, Xi-Ming Xu, Sui-Yi Wu, Zi-Cheng Zhang, Fei Wang, Yi-Lin Yang, Ming Li, Xian-Zhao Wei
Low-intensity pulsed ultrasound (LIPUS) has been found to accelerate the healing process of spinal fusion via a process closely related to osteoblast differentiation and migration. Sonic hedgehog (Shh) signaling plays an important role in development and homeostasis, including a critical function in bone formation. However, its role in spinal fusion during LIPUS treatment is still unknown. This study showed that LIPUS treatment after spinal fusion surgery increased bone formation. The increased bone mass under LIPUS treatment appeared to result from the increased migration and proliferation of osteoblasts, resulting from upregulation of the Shh signaling pathway...
May 2018: Bone
https://www.readbyqxmd.com/read/29383687/transcranial-ultrasound-stimulation-improves-long-term-functional-outcomes-and-protects-against-brain-damage-in-traumatic-brain-injury
#16
Szu-Fu Chen, Wei-Shen Su, Chun-Hu Wu, Tsuo-Hung Lan, Feng-Yi Yang
The purpose of this study was to assess the long-term treatment efficacy of low-intensity pulsed ultrasound (LIPUS) on functional outcomes, brain edema, and the possible involvement of reactions in mice following traumatic brain injury (TBI). Mice subjected to controlled cortical impact injury received LIPUS treatment daily for a period of 4 weeks. The effects of LIPUS on edema were detected by MR imaging in the mouse brain at 148 days following TBI. Long-term functional outcomes of LIPUS stimulation were evaluated by behavioral analyses...
January 30, 2018: Molecular Neurobiology
https://www.readbyqxmd.com/read/29273088/low-intensity-pulsed-ultrasound-prevents-muscle-atrophy-induced-by-type-1-diabetes-in-rats
#17
Liang Tang, Nan Li, Wenqi Jian, Yiting Kang, Bo Yin, Shuxin Sun, Jianzhong Guo, Lijun Sun, Dean Ta
BACKGROUND: Type 1 diabetes mellitus (T1DM) induces serious skeletal muscle atrophy. Low-intensity pulsed ultrasound (LIPUS) is a common treatment for skeletal muscle injury and is effective in accelerating the rate of muscle growth. However, to the best of our knowledge, whether LIPUS can improve skeletal muscle atrophy in type 1 diabetic rats has not been investigated. METHODS: The rats were randomly divided into four groups: the normal control group (NC); the sham-treated diabetic control group (DC); the diabetic, insulin-treated group (DI) as a positive control; and the diabetic LIPUS therapy group (DL)...
December 22, 2017: Skeletal Muscle
https://www.readbyqxmd.com/read/29258619/effects-of-low-intensity-pulsed-ultrasound-lipus-pretreated-human-amnion-derived-mesenchymal-stem-cell-had-msc-transplantation-on-primary-ovarian-insufficiency-in-rats
#18
Li Ling, Xiushan Feng, Tianqin Wei, Yan Wang, Yaping Wang, Wenqian Zhang, Lianli He, Ziling Wang, Qianru Zeng, Zhengai Xiong
BACKGROUND: Human amnion-derived mesenchymal stem cells (hAD-MSCs) have the features of mesenchymal stem cells (MSCs). Low-intensity pulsed ultrasound (LIPUS) can promote the expression of various growth factors and anti-inflammatory molecules that are necessary to keep the follicle growing and to reduce granulosa cell (GC) apoptosis in the ovary. This study aims to explore the effects of LIPUS-pretreated hAD-MSC transplantation on chemotherapy-induced primary ovarian insufficiency (POI) in rats...
December 19, 2017: Stem Cell Research & Therapy
https://www.readbyqxmd.com/read/29237506/tgf-%C3%AE-1-induced-chondrogenesis-of-bone-marrow-mesenchymal-stem-cells-is-promoted-by-low-intensity-pulsed-ultrasound-through-the-integrin-mtor-signaling-pathway
#19
Peng Xia, Xiaoju Wang, Yanping Qu, Qiang Lin, Kai Cheng, Mingxia Gao, Shasha Ren, Tingting Zhang, Xueping Li
BACKGROUND: Low-intensity pulsed ultrasound (LIPUS) is a mechanical stimulus that plays a key role in regulating the differentiation of bone marrow mesenchymal stem cells (BMSCs). However, the way in which it affects the chondrogenic differentiation of BMSCs remains unknown. In this study, we aimed to investigate whether LIPUS is able to influence TGF-β1-induced chondrogenesis of BMSCs through the integrin-mechanistic target of the Rapamycin (mTOR) signaling pathway. METHODS: BMSCs were isolated from rat bone marrow and cultured in either standard or TGF-β1-treated culture medium...
December 13, 2017: Stem Cell Research & Therapy
https://www.readbyqxmd.com/read/29199330/-effect-of-low-intensity-pulsed-ultrasound-combined-with-triamcinolone-acetonide-on-oral-mucosal-ulcer-healing-in-syrian-hamster
#20
Tian-Ming Feng, Ming-Cong Yang, Lan-Lan Pan, Chen-Xi Fei, Chao Wang, Xue-Rong Xiang, Jun Sun
PURPOSE: To evaluate the effect of low-intensity pulsed ultrasound(LIPUS) combined with triamcinolone acetonide on oral mucosal ulcer in syrian hamster in several ways, including healing time, contents of superoxide dismutase(SOD)and malondialdehyde (MDA). METHODS: Sixty syrian hamsters were randomly divided into 5 groups, including a baseline group (containing a normal baseline group and a model baseline group, n=6) and 4 experimental groups (LIPUS processing and drug use group, LIPUS group, drug group and a normal control group without any processing, n=12)...
August 2017: Shanghai Kou Qiang Yi Xue, Shanghai Journal of Stomatology
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