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Dengkui DU, Shirong Chen, Gang Yi, Pan Wang, Ying Tang, Liwen Zheng, Junnan Chen
Objective To investigate the effect of low-intensity pulsed ultrasound (LIPUS) on the extracellular matrix synthesis of human osteoarthritis (OA) chondrocytes and explore the underlying mechanism. Methods Human osteoarthritis chondrocytes were collected from abandoned articular cartilage. Then the cells were cultured and identified by toluidine blue staining and immunocytochemical staining of type 2 collagen. The passage 2 cells were randomly divided into 3 groups: control OA group, 30 mW/cm(2) LIPUS-treated OA group, 30 mW/cm(2) LIPUS combined with 5 μmol/L LY294002-treated OA group...
November 2016: Xi Bao Yu Fen Zi Mian Yi Xue za Zhi, Chinese Journal of Cellular and Molecular Immunology
Takashi Nishida, Satoshi Kubota, Eriko Aoyama, Nobuyasu Yamanaka, Karen M Lyons, Masaharu Takigawa
OBJECTIVE: CCN family protein 2/connective tissue growth factor (CCN2/CTGF) promotes cartilage regeneration in experimental osteoarthritis (OA) models. However, CCN2 production is very low in articular cartilage. The aim of this study was to investigate whether or not CCN2 was promoted by cultured chondrocytes treated with low-intensity pulsed ultrasound (LIPUS) and to clarify its mechanism. METHODS: Human chondrocytic cell line HCS-2/8, rat primary epiphyseal and articular cartilage cells, and Ccn2-deficient chondrocytes that impaired chondrocyte differentiation, were treated with LIPUS for 20 min at 3...
October 8, 2016: Osteoarthritis and Cartilage
Julio J Jauregui, Anthony V Ventimiglia, Preston W Grieco, David B Frumberg, John E Herzenberg
BACKGROUND: Limb lengthening with external fixation is performed to treat patients with leg length discrepancy or short stature. Although the procedure has a high rate of success, one potential drawback from limb lengthening is the amount of time spent in the fixation device while regenerate bone consolidates. Although some studies have assessed different treatment modalities, there has not been a study that has systematically evaluated whether low intensity pulsed ultrasound (LIPUS) or pulsed electromagnetic fields (PEMF) have significant effects on regenerate bone growth...
September 29, 2016: BMC Musculoskeletal Disorders
Kyozo Sasaki, Mitsuru Motoyoshi, Eri Horinuki, Yoshinori Arai, Noriyoshi Shimizu
This study examined the effects of a bite-jumping appliance combined with low-intensity pulsed ultrasound (LIPUS) stimulation on the mandibular condyle of growing rats using micro CT (mCT) and histological examinations. Twelve Wistar rats were divided into three groups of four individuals each: Group 1 was an untreated control group, Group 2 received bite-jumping appliances, and Group 3 received bite-jumping appliances and LIPUS stimulation (15 min/day, 2 weeks) to the temporomandibular region. We measured the length and three-dimensional bone volume of each rat's mandibular condyle using mCT...
2016: Journal of Oral Science
Peter Nolte, Robert Anderson, Elton Strauss, Zhe Wang, Liuyi Hu, Zekun Xu, R Grant Steen
INTRODUCTION: Whether to treat metatarsal fractures conservatively or surgically is controversial. We test a hypothesis that metatarsal fractures treated conservatively with non-invasive low-intensity pulsed ultrasound (LIPUS) obtain heal rates comparable to current surgical techniques. PATIENTS AND METHODS: This is a retrospective observational cohort study, using patient outcomes from a prospectively-collected LIPUS registry required by the U.S. Food & Drug Administration...
September 13, 2016: Injury
Yuan Yue, Xingmei Yang, Liang Zhang, Xun Xiao, Neel R Nabar, Yunfeng Lin, Liang Hao, Dongjiao Zhang, Jingyi Huo, Jingle Li, Xiaoxiao Cai, Min Wang
OBJECTIVES: Peripheral nerve injuries are a common occurrence, resulting in considerable patient suffering; it also represents a major economic burden on society. To improve treatment options following peripheral nerve injuries, scientists aim to find a way to promote Schwann cell (SC) myelination to help nerves to carry out their functions effectively. In this study, we investigated myelination ability of SCs, regulated by co-culture with adipose-derived stem cells (ASCs) or low-intensity pulsed ultrasound (LIPUS), and synergistic effects of combined treatments...
September 14, 2016: Cell Proliferation
Shoki Yamaguchi, Tomoki Aoyama, Akira Ito, Momoko Nagai, Hirotaka Iijima, Junichi Tajino, Xiangkai Zhang, Kiyan Wataru, Hiroshi Kuroki
We investigated the effect of low-intensity pulsed ultrasound (LIPUS) treatment combined with mesenchymal stromal cell (MSC) injection for cartilage repair and subchondral bone reconstitution for treatment of osteochondral defects. An osteochondral defect was created on both femur grooves of Wistar rats. Four weeks later, bone marrow MSCs were injected into the right knee joint. The rats were divided into two intervention groups: without or with LIPUS irradiation. Cartilage repair was evaluated histologically based on the Wakitani cartilage repair score...
September 3, 2016: Ultrasound in Medicine & Biology
Xuan Zhou, Nathan J Castro, Wei Zhu, Haitao Cui, Mitra Aliabouzar, Kausik Sarkar, Lijie Grace Zhang
3D printing and ultrasound techniques are showing great promise in the evolution of human musculoskeletal tissue repair and regeneration medicine. The uniqueness of the present study was to combine low intensity pulsed ultrasound (LIPUS) and advanced 3D printing techniques to synergistically improve growth and osteogenic differentiation of human mesenchymal stem cells (MSC). Specifically, polyethylene glycol diacrylate bioinks containing cell adhesive Arginine-Glycine-Aspartic acid-Serene (RGDS) peptide and/or nanocrystalline hydroxyapatite (nHA) were used to fabricate 3D scaffolds with different geometric patterns via novel table-top stereolithography 3D printer...
2016: Scientific Reports
Lu Zhao, Yi Feng, Hong Hu, Aiwei Shi, Lei Zhang, Mingxi Wan
Enhancing the action of nerve growth factor (NGF) is a potential therapeutic approach to neural regeneration. To facilitate neural regeneration, we investigated whether combining low-intensity pulsed ultrasound (LIPUS) and NGF could promote neurite outgrowth, an essential process in neural regeneration. In the present study, PC12 cells were subjected to a combination of LIPUS (1 MHz, 30 or 50 mW/cm(2), 20% duty cycle and 100-Hz pulse repetition frequency, 10 min every other day) and NGF (50 ng/mL) treatment, and then neurite outgrowth was compared...
September 1, 2016: Ultrasound in Medicine & Biology
Daniel Lipus, Amit Vikram, Daniel E Ross, Kyle Bibby
We report here the 1,882,100-bp draft genome sequence of Methanohalophilus mahii strain DAL1, recovered from Marcellus Shale hydraulic fracturing-produced water using metagenomic contig binning. Genome annotation revealed several key methanogenesis genes and provides valuable information on archaeal activity associated with hydraulic fracturing-produced water environments.
2016: Genome Announcements
Lisa K Marriott, Adam C Lipus, Laurie Choate, Jamie Smith, Leigh Coppola, William E Cameron, Jackilen Shannon
Engaging community members in research can help cultivate effective partnerships while providing experiential training and continuing education opportunities. Several studies have involved communities in this way, though many have been small in the scale of community involvement or have included little detail of the institutional review board process by which community members became approved researchers in the study. This article presents findings on an evaluation of the training procedures and experiences of 703 first-time community-based volunteer researchers who were recruited in their communities and trained on-site to enroll research participants, collect data, and provide individualized consultation of results at travelling health education and research fairs...
December 2015: Pedagogy in Health Promotion
Sjoerd Rutten, Michel P J van den Bekerom, Inger N Sierevelt, Petrus A Nolte
BACKGROUND: Low-intensity pulsed ultrasound (LIPUS) is frequently used to enhance or to accelerate fracture-healing, but its clinical role and effectiveness as a treatment modality remain uncertain. We performed a systematic review and meta-analysis of randomized controlled trials to determine the efficiency of LIPUS on bone-healing and/or fracture union, as well as on functional recovery. METHODS: The databases of PubMed/MEDLINE, Cochrane Central Register of Controlled Trials, CINAHL, Web of Science, and Embase were searched for trials concerning LIPUS stimulation and bone-healing or fracture repair, in any language, published from the inception of the database to January 2, 2015...
March 29, 2016: JBJS Rev
Amit Vikram, Daniel Lipus, Kyle Bibby
Controlling microbial activity is a primary concern during the management of the large volumes of wastewater (produced water) generated during high-volume hydraulic fracturing. In this study we analyzed the transcriptional activity (metatranscriptomes) of three produced water samples from the Marcellus Shale. The goal of this study was to describe active metabolic pathways of industrial concern for produced water management and reuse, and to improve understanding of produced water microbial activity. Metatranscriptome analysis revealed active biofilm formation, sulfide production, and stress management mechanisms of the produced water microbial communities...
October 2016: Microbial Ecology
Masatoshi Higuchi, Akinori Moroi, Kunio Yoshizawa, Akihiko Kosaka, Hiroumi Ikawa, Ran Iguchi, Yuriko Saida, Asami Hotta, Takamitsu Tsutsui, Koichiro Ueki
PURPOSE: The purpose of this study was to compare bone regenerative capability following use of polytetrafluoroethylene (ePTFE) membrane against that when various densities of pore titanium meshes are used with and without low intensity pulsed ultrasound (LIPUS). MATERIALS AND METHODS: Adult male white rabbits were divided into 8 groups. In 4 groups, after incising along the nasal bone, four 3 × 8 mm bone defects were made in both sides and covered by an ePTFE membrane (group E: n = 15), a high density pore titanium mesh (group H: n = 15), a low density pore titanium mesh (group L: n = 15), and no mesh (control) (group C: n = 15)...
September 2016: Journal of Cranio-maxillo-facial Surgery
Kouki Hidaka, Chihiro Miyamoto, Akira Kawata, Makiko Saita, Ryota Kawamata, Yojiro Maehata, Masato Minabe, Satoko W Takahashi, Shun-Suke Takahashi, Yuko Mikuni-Takagaki
OBJECTIVE: We reported at the previous annual meeting that LIPUS treatment of the molar tooth sockets of retired breeder rats accelerated alveolar bone healing, and that associated humoral effects were seen with elevated blood flow. Namely, LIPUS induced VEGF/angiogenesis along with elevated baseline blood flow rate, which was further associated with a sudden depression of blood flow rate in the socket immediately after cessation of LIPUS treatment. Prior injection with EP4 PGE2 receptor antagonist, but not EP3 antagonist, abolished this LIPUS-induced depression, and topical application of PGE2 to the socket epithelium mimicked the LIPUS-induced depression...
August 2016: Journal of Orthopaedic Trauma
Koji Naruse, Masataka Uchino, Noriko Hirakawa, Masahiro Toyama, Genyo Miyajima, Manabu Mukai, Ken Urabe, Kentaro Uchida, Moritoshi Itoman
OBJECTIVE: We have conducted a basic study on the influences on ultrasonic properties when LIPUS is applied through wound dressing. According to the results of ex vivo experiments conducted to date, LIPUS showed ultrasonic properties such as transmittance, coefficient of transmission, and a non-uniformity ratio through film wound dressing better than other wound dressing, and it was considered that LIPUS's effect for fracture healing was not influenced by film wound dressing. Then, we discussed the influence on the effect of LIPUS through film wound dressing...
August 2016: Journal of Orthopaedic Trauma
Chieko Noguchi, Hironobu Koseki
Photocatalysis with anatase-type titanium dioxide (TiO2) under ultraviolet has a well-recognized bactericidal effect. The purpose of the present study was to evaluate the photocatalytic bactericidal effects of TiO2 on Staphylococcus epidermidis (ATCC35984) caused by Low Intensity Pulsed Ultrasound (LIPUS) associated with bio-implant-related infections. The photocatalytic properties of the TiO2 films were confirmed by the degradation of an aqueous solution of methylene blue. The disks were seeded with cultured Staphylococcus epidermidis and irradiated by LIPUS...
August 2016: Journal of Orthopaedic Trauma
Joji Kusuyama, Chang Hwan Seong, Tomokazu Ohnishi, Kenjiro Bandow, Tetsuya Matsuguchi
Mesenchymal Stem Cells (MSCs) are pluripotent cells that can be differentiated as osteoblasts, adipocytes, myocytes or chondrocytes depending on the culture condition. However, MSCs are known to lose their differentiation potency after long-term culture. Development of a new cell culture method to maintain their stemness is required for successful application of MSCs. Here, we revealed that low-intensity pulsed ultrasound (LIPUS) stimulation was useful for maintaining the MSC stemness as LIPUS inhibited the loss of osteogenic differentiation potency of osteo-progenitor cells induced by serial subculture...
August 2016: Journal of Orthopaedic Trauma
Nobuo Suzuki, Taizo Hanmoto, Mika Ikegame, Yukihiro Furusawa, Yoshiaki Tabuchi, Takashi Kondo, Kei-Ichiro Kitamura, Toshio Sekiguchi, Yuko Mikuni-Takagaki, Atsuhiko Hattori
OBJECTIVE: Low-intensity pulsed ultrasound (LIPUS) provides noninvasive therapeutic treatment to accelerate fracture repair and distraction osteogenesis. However, most studies concerning the influence of LIPUS on bone metabolism have been conducted in vivo systems using osteoblastic cells. Therefore, details of the direct effect of LIPUS on osteoclasts are not yet fully understood. Teleost scale is a calcified tissue that contains osteoclasts and osteoblasts. Its bone matrix consists of type I collagen and hydroxyapatite, and is similar to that of mammalian bone...
August 2016: Journal of Orthopaedic Trauma
Takahiro Niikura, Sang Yang Lee, Takashi Iwakura, Masahiro Kurosaka
BACKGROUND: The Masquelet technique is a relatively new option in the management of bone defects. It consists of 2-stage surgery. At first surgery, a cement spacer is placed to fill the bone defect. After a waiting period of 6-8 weeks, a pseudo-synovial membrane is formed around the cement. The induced membrane is rich in vascularity and osteogenic activity. At second surgery, the cement is replaced with an autologous bone graft and the graft is packed in the membrane. To harvest large quantities of bone graft, the reamer irrigator aspirator (RIA) is utilized...
August 2016: Journal of Orthopaedic Trauma
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