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https://www.readbyqxmd.com/read/28427634/enhanced-porosity-and-permeability-of-three-dimensional-alginate-scaffolds-via-acoustic-microstreaming-induced-by-low-intensity-pulsed-ultrasound
#1
Gepu Guo, Yong Ma, Yang Guo, Chunbing Zhang, Xiasheng Guo, Juan Tu, Alfred C H Yu, Junru Wu, Dong Zhang
The shear stress resulting from the microstreaming induced by low-intensity pulsed ultrasound (LIPUS) has been often used to improve the permeability of cell membrane or porous engineering scaffolds. In the present study, three-dimensional (3-D) scaffold culture systems were constituted to simulate the in vivo microenvironment, providing benefits for cell growth. In order to investigate the mechanism underlying the enhanced porosity and permeability of the 3-D alginate scaffolds by using LIPUS with varied acoustic intensities, two quantitative imaging techniques (i...
July 2017: Ultrasonics Sonochemistry
https://www.readbyqxmd.com/read/28391752/low-intensity-pulsed-ultrasound-for-nonoperative-treatment-of-scaphoid-nonunions-a-meta-analysis
#2
Edward W Seger, Julio J Jauregui, Steven A Horton, Gerardo Davalos, Erika Kuehn, Michael A Stracher
BACKGROUND: Scaphoid fractures progress to nonunion rates of up to 15% when non-displaced, and are even more frequent when the fracture is displaced. Standard treatment in these cases is surgery; however, individuals unable to undergo this operation, or for those who wish to try more conservative measures, there may be benefit from nonoperative options. Of these, low-intensity pulsed ultrasound (LIPUS) has been shown to improve fracture nonunion healing. The purpose of this study was to perform a comprehensive meta-analysis of relevant literature to determine success of the use of LIPUS for treatment of scaphoid nonunion...
April 1, 2017: Hand: Official Journal of the American Association for Hand Surgery
https://www.readbyqxmd.com/read/28391380/low-intensity-pulsed-ultrasound-is-effective-for-progressive-stage-lumbar-spondylolysis-with-mri-high-signal-change
#3
Hideyuki Arima, Yoshiji Suzuki, Daisuke Togawa, Yuki Mihara, Hideyuki Murata, Yukihiro Matsuyama
PURPOSE: This study aimed to investigate the treatment effects of low-intensity pulsed ultrasound (LIPUS) on progressive-stage spondylolysis. Spondylolysis is a stress fracture of the pars interarticularis. Based on the results of computed tomography, spondylolysis was classified into three categories: early, progressive, and terminal. Bone healing was prolonged or not obtained in progressive-stage spondylolysis. The progression of spondylolysis to nonunion has been associated with an increased incidence of spondylolisthesis...
April 8, 2017: European Spine Journal
https://www.readbyqxmd.com/read/28385939/low-intensity-pulsed-ultrasound-does-not-influence-bone-healing-by-distraction-osteogenesis-a-multicentre-double-blind-randomised-control-trial
#4
A H R W Simpson, G Keenan, S Nayagam, R M Atkins, D Marsh, N D Clement
AIMS: The aim of this double-blind prospective randomised controlled trial was to assess whether low intensity pulsed ultrasound (LIPUS) accelerated or enhanced the rate of bone healing in adult patients undergoing distraction osteogenesis. PATIENTS AND METHODS: A total of 62 adult patients undergoing limb lengthening or bone transport by distraction osteogenesis were randomised to treatment with either an active (n = 32) or a placebo (n = 30) ultrasound device...
April 2017: Bone & Joint Journal
https://www.readbyqxmd.com/read/28378753/low-intensity-pulsed-ultrasound-lipus-prevents-periprosthetic-inflammatory-loosening-through-fbxl2-traf6-ubiquitination-pathway
#5
Xiang Zhao, Gangsheng Zhao, Zhongli Shi, Chenhe Zhou, Yunlin Chen, Bin Hu, Shigui Yan
Previous studies have shown that Low intensity pulsed ultrasound(LIPUS) prevents polyethylene-debris-induced periprosthetic loosening in vivo, but the details of the mechanism by which it does so remain unclear. In this article, we used polyethylene debris induced RAW 264.7 cells as the in vitro model, and tested the effect of LIPUS on this model. Changes in the level of inflammatory cytokines, cell proliferation, and apoptosis were assessed. Gene overexpression and siRNA technique were applied, and the levels of expression of FBXL2, TRAF6, ERK, and related inflammatory cytokines were also measured...
April 5, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28348110/low-intensity-pulsed-ultrasound-for-bone-healing-systematic-review-of-randomized-controlled-trials
#6
REVIEW
Stefan Schandelmaier, Alka Kaushal, Lyubov Lytvyn, Diane Heels-Ansdell, Reed A C Siemieniuk, Thomas Agoritsas, Gordon H Guyatt, Per O Vandvik, Rachel Couban, Brent Mollon, Jason W Busse
Objective To determine the efficacy of low intensity pulsed ultrasound (LIPUS) for healing of fracture or osteotomy.Design Systematic review and meta-analysis.Data sources Medline, Embase, CINAHL, Cochrane Central Register of Controlled Trials, and trial registries up to November 2016.Study selection Randomized controlled trials of LIPUS compared with sham device or no device in patients with any kind of fracture or osteotomy.Review methods Two independent reviewers identified studies, extracted data, and assessed risk of bias...
February 22, 2017: BMJ: British Medical Journal
https://www.readbyqxmd.com/read/28331074/osteopontin-inhibits-osteoblast-responsiveness-through-the-downregulation-of-focal-adhesion-kinase-mediated-by-the-induction-of-low-molecular-weight-protein-tyrosine-phosphatase
#7
Joji Kusuyama, Kenjiro Bandow, Tomokazu Ohnishi, Mitsuhiro Hisadome, Kaori Shima, Ichiro Semba, Tetsuya Matsuguchi
Osteopontin (OPN) is an osteogenic marker protein. Osteoblast functions are affected by inflammatory cytokines and pathological conditions. OPN is highly expressed in bone legions such as rheumatoid arthritis. However, local regulatory effects of OPN on osteoblasts remain ambiguous. Here, we examined how OPN influences osteoblast responses to mechanical stress and growth factors. Expression of NO synthase 1 (Nos1) and Nos2 was increased by low intensity pulsed ultrasound (LIPUS) in MC3T3-E1 cells and primary osteoblasts...
March 22, 2017: Molecular Biology of the Cell
https://www.readbyqxmd.com/read/28302780/draft-genome-sequence-of-pseudomonas-sp-bdal1-reconstructed-from-a-bakken-shale-hydraulic-fracturing-produced-water-storage-tank-metagenome
#8
Daniel Lipus, Daniel Ross, Kyle Bibby, Djuna Gulliver
We report the 5,425,832 bp draft genome of Pseudomonas sp. strain BDAL1, recovered from a Bakken shale hydraulic fracturing-produced water tank metagenome. Genome annotation revealed several key biofilm formation genes and osmotic stress response mechanisms necessary for survival in hydraulic fracturing-produced water.
March 16, 2017: Genome Announcements
https://www.readbyqxmd.com/read/28276640/prospective-review-of-mesenchymal-stem-cells-differentiation-into-osteoblasts
#9
REVIEW
Priyanka Garg, Matthew M Mazur, Amy C Buck, Meghan E Wandtke, Jiayong Liu, Nabil A Ebraheim
Stem cell research has been a popular topic in the past few decades. This review aims to discuss factors that help regulate, induce, and enhance mesenchymal stem cell (MSC) differentiation into osteoblasts for bone regeneration. The factors analyzed include bone morphogenic protein (BMP), transforming growth factor β (TGF-β), stromal cell-derived factor 1 (SDF-1), insulin-like growth factor type 1 (IGF-1), histone demethylase JMJD3, cyclin dependent kinase 1 (CDK1), fucoidan, Runx2 transcription factor, and TAZ transcriptional coactivator...
February 2017: Orthopaedic Surgery
https://www.readbyqxmd.com/read/28239034/effects-of-low-intensity-pulsed-ultrasound-on-osteoclasts-analysis-with-goldfish-scales-as-a-model-of-bone
#10
Taizo Hanmoto, Yoshiaki Tabuchi, Mika Ikegame, Takashi Kondo, Kei-Ichiro Kitamura, Masato Endo, Isao Kobayashi, Hiroyuki Mishima, Toshio Sekiguchi, Makoto Urata, Azusa Seki, Sachiko Yano, Atsuhiko Hattori, Nobuo Suzuki
The effects of low-intensity pulsed ultrasound (LIPUS) on osteoclastogenesis were examined using fish scales that had both osteoclasts and osteoblasts. The binding of the receptor activator of NF-κB ligand (RANKL) in osteoblasts to the receptor activator of NF-κB (RANK) in osteoclasts induced osteoclastogenesis. Therefore, we focused on RANK/RANKL signaling. After 6 h of incubation following LIPUS treatment, mRNA expression of RANKL increased significantly. Resulting from the increased RANKL mRNA level, the expression of transcription-regulating factors significantly increased after 6 h of incubation, and then the mRNA expression of functional genes was significantly up-regulated after 12 h of incubation...
2017: Biomedical Research
https://www.readbyqxmd.com/read/28228381/low-intensity-pulsed-ultrasound-lipus-for-bone-healing-a-clinical-practice-guideline
#11
Rudolf W Poolman, Thomas Agoritsas, Reed A C Siemieniuk, Ian A Harris, Inger B Schipper, Brent Mollon, Maureen Smith, Alexandra Albin, Sally Nador, Will Sasges, Stefan Schandelmaier, Lyubov Lytvyn, Ton Kuijpers, Loes W A H van Beers, Michael H J Verhofstad, Per Olav Vandvik
No abstract text is available yet for this article.
February 21, 2017: BMJ: British Medical Journal
https://www.readbyqxmd.com/read/28197400/effect-of-gingival-fibroblasts-and-ultrasound-on-dogs-root-resorption-during-orthodontic-treatment
#12
Jacqueline Crossman, Ali H Hassan, Ali Saleem, Nayef Felemban, Saleh Aldaghreer, Elham Fawzi, Mamdouh Farid, Khaled Abdel-Ghaffar, Ausama Gargoum, Tarek El-Bialy
OBJECTIVES: To investigate the effect of using osteogenic induced gingival fibroblasts (OIGFs) and low intensity pulsed ultrasound (LIPUS) on root resorption lacunae volume and cementum thickness in beagle dogs that received orthodontic tooth movement. MATERIALS AND METHODS: Seven beagle dogs were used, from which gingival cells (GCs) were obtained and were induced osteogenically to produce OIGFs. Each third and fourth premolar was randomly assigned to one of the five groups, namely, LIPUS, OIGFs, bone morphogenetic protein-2 (BMP-2), OIGFs + LIPUS, and control...
January 2017: Journal of Orthodontic Science
https://www.readbyqxmd.com/read/28188834/amelioration-of-cerebellar-dysfunction-in-rats-following-postnatal-ethanol-exposure-using-low-intensity-pulsed-ultrasound
#13
Hiva Mohammadi Bolbanabad, Enayat Anvari, Mohammad Jafar Rezai, Ardashir Moayeri, Mohammad Reza Kaffashian
BACKGROUND: The neonatal development stage of the cerebellum in rats is equivalent to a human foetus in the third trimester of pregnancy. In this stage, cell proliferation, migration, differentiation, and synaptogenesis occur. Clinical and experimental findings have shown that ethanol exposure during brain development causes a variety of disruptions to the brain, including neurogenesis depression, delayed neuronal migration, changes in neurotransmitter synthesis, and neuronal depletion...
February 7, 2017: Journal of Chemical Neuroanatomy
https://www.readbyqxmd.com/read/28159795/predominance-and-metabolic-potential-of-halanaerobium-spp-in-produced-water-from-hydraulically-fractured-marcellus-shale-wells
#14
Daniel Lipus, Amit Vikram, Daniel Ross, Daniel Bain, Djuna Gulliver, Richard Hammack, Kyle Bibby
Microbial activity in the produced water from hydraulically fractured oil and gas wells may potentially interfere with hydrocarbon production and cause damage to the well and surface infrastructure via corrosion, sulfide release, and fouling. In this study, we surveyed the microbial abundance and community structure of produced water sampled from 42 Marcellus Shale wells in southwestern Pennsylvania (well age ranged from 150 to 1,846 days) to better understand the microbial diversity of produced water. We sequenced the V4 region of the 16S rRNA gene to assess taxonomy and utilized quantitative PCR (qPCR) to evaluate the microbial abundance across all 42 produced water samples...
April 15, 2017: Applied and Environmental Microbiology
https://www.readbyqxmd.com/read/28134628/when-is-a-fracture-not-fresh-aligning-reimbursement-with-patient-outcome-after-treatment-with-low-intensity-pulsed-ultrasound
#15
Robert Zura, Zekun Jack Xu, Gregory J Della Rocca, Samir Mehta, R Grant Steen
OBJECTIVE: The clinical value of low-intensity pulsed ultrasound (LIPUS) for fresh fracture is known. Yet, in the absence of a definition of what "fresh" is, payers have adopted study inclusion criteria drawn from randomized clinical trials as de facto definitions of which patients should be treated, with "fresh" defined as <1 week old. Patients with fracture may thus be ineligible for LIPUS treatment after week 1, which potentially denies access to patients who could benefit from LIPUS...
May 2017: Journal of Orthopaedic Trauma
https://www.readbyqxmd.com/read/28127791/gene-expression-profiling-analysis-of-the-effects-of-low-intensity-pulsed-ultrasound-on-induced-pluripotent-stem-cells-derived-neural-crest-stem-cells
#16
Bin Xia, Yang Zou, Zhiling Xu, Yonggang Lv
Low-intensity pulsed ultrasound (LIPUS) is a non-invasive technique that has been shown to affect the cell proliferation, migration, differentiation, and promote the regeneration of damaged peripheral nerve. Our previous studies had proved that LIPUS can significantly promote the neural differentiation of induced pluripotent stem cells-derived neural crest stem cells (iPSCs-NCSCs) and enhance the repair of rat transected sciatic nerve. To further explore the underlying mechanisms of LIPUS treatment on iPSCs-NCSCs, this study reported the gene expression profiling analysis of iPSCs-NCSCs before and after LIPUS treatment using RNA-sequencing (RNA-Seq) method...
January 26, 2017: Biotechnology and Applied Biochemistry
https://www.readbyqxmd.com/read/28063227/lipus-suppressed-lps-induced-il-1%C3%AE-through-the-inhibition-of-nf-%C3%AE%C2%BAb-nuclear-translocation-via-at1-plc%C3%AE-pathway-in-mc3t3-e1-cells
#17
Mayu Nagao, Natsuko Tanabe, Soichiro Manaka, Masako Naito, Jumpei Sekino, Tadahiro Takayama, Takayuki Kawato, Go Torigoe, Shunichiro Kato, Naoya Tsukune, Masao Maeno, Naoto Suzuki, Shuichi Sato
Inflammatory cytokines, interleukin (IL)-1, IL-6, and TNF-α, are involved in inflammatory bone diseases such as rheumatoid osteoarthritis and periodontal disease. Particularly, periodontal disease, which destroys alveolar bone, is stimulated by lipopolysaccharide (LPS). Low-intensity pulsed ultrasound (LIPUS) is used for bone healing in orthopedics and dental treatments. However, the mechanism underlying effects of LIPUS on LPS-induced inflammatory cytokine are not well understood. We therefore aimed to investigate the role of LIPUS on LPS-induced IL-1α production...
January 7, 2017: Journal of Cellular Physiology
https://www.readbyqxmd.com/read/27998203/comment-to-a-bmj-editorial
#18
EDITORIAL
Per Aspenberg
No abstract text is available yet for this article.
February 2017: Acta Orthopaedica
https://www.readbyqxmd.com/read/27942237/design-evolution-enhances-patient-compliance-for-low-intensity-pulsed-ultrasound-device-usage
#19
Neill M Pounder, John T Jones, Kevin J Tanis
Poor patient compliance or nonadherence with prescribed treatments can have a significant unfavorable impact on medical costs and clinical outcomes. In the current study, voice-of-the-customer research was conducted to aid in the development of a next-generation low-intensity pulsed ultrasound (LIPUS) bone healing product. An opportunity to improve patient compliance reporting was identified, resulting in the incorporation into the next-generation device of a visual calendar that provides direct feedback to the patient, indicating days for which they successfully completed treatment...
2016: Medical Devices: Evidence and Research
https://www.readbyqxmd.com/read/27883051/lipid-coated-microbubbles-and-low-intensity-pulsed-ultrasound-enhance-chondrogenesis-of-human-mesenchymal-stem-cells-in-3d-printed-scaffolds
#20
Mitra Aliabouzar, Lijie Grace Zhang, Kausik Sarkar
Lipid-coated microbubbles are used to enhance ultrasound imaging and drug delivery. Here we apply these microbubbles along with low intensity pulsed ultrasound (LIPUS) for the first time to enhance proliferation and chondrogenic differentiation of human mesenchymal stem cells (hMSCs) in a 3D printed poly-(ethylene glycol)-diacrylate (PEG-DA) hydrogel scaffold. The hMSC proliferation increased up to 40% after 5 days of culture in the presence of 0.5% (v/v) microbubbles and LIPUS in contrast to 18% with LIPUS alone...
November 24, 2016: Scientific Reports
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