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https://www.readbyqxmd.com/read/28632669/effect-of-low-intensity-pulsed-ultrasound-lipus-on-endochondral-ossification-via-the-wnt-signaling-pathway
#1
Ken Kumagai, Yasuteru Yamaguchi, Kazuma Miyatake, Hironori Tanabe, Sosuke Imai, Tomoyuki Saito
OBJECTIVE: We have previously demonstrated that LIPUS stimulates endochondral ossification with decreased expression of sclerostin. The Wnt signaling pathway was examined in vitro to further address the effect of LIPUS on endochondral ossification. MATERIALS AND METHODS: ATDC5 cells were plated at an initial density of 6 × 10 cells/well in a 6-multiwell plate and cultured in the presence of 5% FBS plus ITS. The bottom of the culture plate was treated every day with LIPUS for 20 minutes...
July 2017: Journal of Orthopaedic Trauma
https://www.readbyqxmd.com/read/28632668/low-intensity-pulsed-ultrasound-lipus-promotes-bmp9-induced-osteogenesis-and-suppresses-inflammatory-responses-in-human-periodontal-ligament-derived-stem-cells
#2
Joji Kusuyama, Toshiaki Nakamura, Tomokazu Ohnishi, Nahoko Eiraku, Kazuyuki Noguchi, Tetsuya Matsuguchi
OBJECTIVE: We previously reported that low intensity pulsed ultrasound (LIPUS) promotes marrow stromal cell (MSC) osteogenesis and suppresses the LPS-induced inflammatory response in osteoblasts. Here, we examined the effects of LIPUS on human periodontal ligament-derived stem cells (hPDLSCs) in chronic inflammatory bone disease, such as periodontitis. MATERIALS AND METHODS: hPDLSCs were collected from 3 healthy third molars. hPDLSCs were induced to differentiate by either recombinant BMP2 or BMP9 with or without daily LIPUS treatment (20 min/d)...
July 2017: Journal of Orthopaedic Trauma
https://www.readbyqxmd.com/read/28632667/study-on-ultrasound-irradiation-induction-of-electrical-potentials-in-bone
#3
Mami Matsukawa, Sayaka Matsukawa
OBJECTIVE: It is well known that applied stress induces electrical potentials in bone. We have experimentally studied whether induced potentials could be found in bone using an ultrasound irradiation system similar to low-intensity pulsed ultrasound (LIPUS). MATERIALS AND METHODS: Circular plate specimens (diameter 10 mm, thickness 1 mm) were obtained from bovine femora in order to determine if bone behaved as a piezoelectric material. Using these specimens, ultrasound receivers were fabricated...
July 2017: Journal of Orthopaedic Trauma
https://www.readbyqxmd.com/read/28632666/change-in-calcaneal-bone-strength-in-long-term-care-residents-treated-with-low-intensity-pulsed-ultrasound
#4
Hiroyasu Okuizumi, Kiyoshi Koyano, Kazuya Kurosawa, Naoto Kamide, Ayako Soyano
OBJECTIVE: We examined the effect of low-intensity pulsed ultrasound (LIPUS) on bone strength in osteoporotic elderly residents. MATERIALS AND METHODS: This was a randomized controlled trial. Elderly residents with osteoporosis in a long-term care facility were treated with LIPUS (oscillation frequency 100 KHz) that involved treatment of the heel bone for 2 minutes a day. The selection of the treated heel was decided randomly with a personal computer. The heel bone mineral density before and after treatment was measured by calcaneal quantitative ultrasound (GE-1000 Express)...
July 2017: Journal of Orthopaedic Trauma
https://www.readbyqxmd.com/read/28632665/effect-of-low-intensity-pulsed-ultrasound-on-bone-healing-at-osteotomy-sites-after-open-wedge-high-tibial-osteotomy
#5
Kenichi Goshima, Takeshi Sawaguchi, Kenji Shigemoto, Sintaro Iwai, Akira Nakanishi, Ken Ueoka
OBJECTIVE: The purpose of this study was to evaluate the effects of low-intensity pulsed ultrasound (LIPUS) on bone healing at osteotomy sites after open wedge high tibial osteotomy (OWHTO). MATERIALS AND METHODS: Twenty-six patients underwent OWHTO without bone grafting. Thirty cases treated with LIPUS (group L) after surgery were compared with 13 cases without LIPUS treatment (group C). We divided the osteotomy gap into the lateral hinge and 4 zones on anteroposterior radiography, and the progression of gap filling was evaluated at 1, 3, and 6 months post-operatively in both groups...
July 2017: Journal of Orthopaedic Trauma
https://www.readbyqxmd.com/read/28632664/outcome-of-low-intensity-pulsed-ultrasound-lipus-for-opening-wedge-high-tibial-osteotomy
#6
Ryusuke Saito, Tsuyoshi Nagase, Tomohiko Tateishi, Sadanori Shimizu, Takuya Kato, Teruhiko Nakagawa, Masamitsu Tsutiya
OBJECTIVE: Opening Wedge High Tibial Osteotomy (OWHTO) for knee osteoarthritis and for osteonecrosis has reported good results. Use of low intensity pulsed ultrasound (LIPUS) after OWHTO had become an option for the treatment of OWHTO since April 2016. The purpose of this study was to examine whether LIPUS has an accelerating effect on synostosis after OWHTO. MATERIALS AND METHODS: The control subjects were 24 patients with a total of 26 knees (9 male with knees and 15 female with 17 knees treated) treated with OWHTO only...
July 2017: Journal of Orthopaedic Trauma
https://www.readbyqxmd.com/read/28632663/an-examination-of-the-factors-related-to-a-reduction-in-the-use-of-low-intensity-pulsed-ultrasound-lipus
#7
Yoshiyasu Uchiyama, Takaaki Tateiwa, Daisuke Nakajima, Naoki Takatori, Masahiko Watanabe
OBJECTIVE: We examined the factors related to a reduction in the use of low-intensity pulsed ultrasound (LIPUS) for patients at both our hospital and other affiliated institutions. MATERIALS AND METHODS: Of 350 patients who used LIPUS at our and other affiliated institutions from May 2010 to April 2015, 279 (168 males, 111 females) were evaluated, after excluding mortalities and those with unexplained bone adhesion. Those patients with LIPUS compliance [calculated as: (number of days LIPUS was used/number of days LIPUS was available) × 100] below 80% were defined as the non-compliant group (170 patients), and those patients with a compliance rate of 80% or above were defined as the compliant group (109 patients)...
July 2017: Journal of Orthopaedic Trauma
https://www.readbyqxmd.com/read/28632662/treatment-of-incomplete-jones-fractures-with-low-intensity-pulsed-ultrasound-lipus
#8
Tateishi Tomohiko, Tsuyoshi Nagase, Teruhiko Nakagawa, Tsuchiya Masamitsu
OBJECTIVE: Stress fractures of the proximal epiphysis of the fifth metatarsal bone (termed Jones fracture) frequently occur in both senior high-school-age and older contestant-level soccer players, and its incidence in Japanese soccer players is higher than that in European players. Surgery is most commonly indicated for a complete fracture, and about 3 months are required before the patient is able to return to the sport. We have performed a "Jones fracture screening" to reduce the incidence of these fractures...
July 2017: Journal of Orthopaedic Trauma
https://www.readbyqxmd.com/read/28632661/efficacy-and-limitations-of-conservative-treatment-for-painful-patella-partita-positioning-of-an-ultrasound-accelerated-fracture-healing-apparatus
#9
Natsuki Sugimura, Daisuke Hirose, Koji Aso, Masashi Izumi, Yusuke Okanoue, Kazuo Kitaoka, Masahiko Ikeuchi
OBJECTIVE: Generally, painful patella partita is conservatively treated, but the usefulness of a low-intensity pulsed ultrasound (LIPUS) healing apparatus is unclear. The objective of this study was to investigate the usefulness of LIPUS for conservative treatment of painful patella partita. SUBJECTS AND METHODS: The subjects included 17 patients diagnosed with painful patella partita. The subjects were divided into 2 groups: those without pain at 6 months (responsive group) and those with pain that persisted for 6 months or longer (non-responsive group)...
July 2017: Journal of Orthopaedic Trauma
https://www.readbyqxmd.com/read/28632660/clinical-experience-with-the-use-of-low-intensity-pulsed-ultrasound-lipus-in-the-treatment-of-atypical-femoral-fractures
#10
Sang Yang Lee, Takahiro Niikura, Takashi Iwakura, Tomoaki Fukui, Ryosuke Kuroda
OBJECTIVE: The aim of this study was to report the clinical results of atypical femoral fractures (AFFs) treated with low-intensity pulsed ultrasound (LIPUS). MATERIALS AND METHODS: The data on AFFs that were surgically treated in our hospital from 2010 to 2016 was retrospectively analyzed. AFF was diagnosed based on the criteria defined by the second report of an ASBMR task force. RESULTS: Seven fractures in 6 cases were included in this study...
July 2017: Journal of Orthopaedic Trauma
https://www.readbyqxmd.com/read/28621452/osteogenic-commitment-and-differentiation-of-human-mesenchymal-stem-cells-by-low-intensity-pulsed-ultrasound-stimulation
#11
Viviana Costa, Valeria Carina, Simona Fontana, Angela De Luca, Francesca Monteleone, Stefania Pagani, Maria Sartori, Stefania Setti, Cesare Faldini, Riccardo Alessandro, Milena Fini, Gianluca Giavaresi
Low-intensity pulsed ultrasound (LIPUS) as an adjuvant therapy in in vitro and in vivo bone engineering has proven to be extremely useful. The present study aimed at investigating the effect of 30 mW/cm(2) LIPUS stimulation on commercially available human mesenchymal stem cells (hMSCs) cultured in basal or osteogenic medium at different experimental time points (7d, 14d, 21d). The hypothesis was that LIPUS would improve the osteogenic differentiation of hMSC and guarantying the maintenance of osteogenic committed fraction, as demonstrated by cell vitality and proteomic analysis...
June 16, 2017: Journal of Cellular Physiology
https://www.readbyqxmd.com/read/28616987/impact-of-low-intensity-pulsed-ultrasound-on-transcript-and-metabolite-abundance-in-saccharomyces-cerevisiae
#12
Michael C Schultz, Jian Zhang, Xian Luo, Oleksandra Savchenko, Liang Li, Michael Deyholos, Jie Chen
The interactions of ultrasound with biological materials are exploited for diagnostic, interventional and therapeutic multiple applications in medicine and can improve biological productivity in industrial biotechnological applications, such as biofuel generation, vaccine and antibodies production. Accordingly, there is great interest in better understanding the biological effects of ultrasound. In this paper, we studied the impact of low-intensity pulsed ultrasound (LIPUS) on RNA expression and metabolism of S...
June 15, 2017: Journal of Proteome Research
https://www.readbyqxmd.com/read/28610471/mechanically-loading-cell-hydrogel-constructs-with-low-intensity-pulsed-ultrasound-for-bone-repair
#13
James A Veronick, Fayekah Assanah, Nicole J Piscopo, Yasemin Kutes, Varun Vyas, Bryan D Huey, Yusuf Khan
Low-intensity pulsed ultrasound (LIPUS) has been shown to be effective for orthopaedic fracture repair and non-union defects but the specific mechanism behind its efficacy is still unknown. Previously we have shown a measurable acoustic radiation force at LIPUS intensities traditionally used for clinical treatment and have applied this force to osteoblastic cells encapsulated in type I collagen hydrogels. Our goal here is to provide insight and inform the appropriate design of a cell therapy approach to bone repair in which osteoblasts are embedded in collagen hydrogels, implanted into a bony defect, and then transdermally stimulated using LIPUS-derived acoustic radiation force to enhance bone formation at the earliest time points after bone defect repair...
June 13, 2017: Tissue Engineering. Part A
https://www.readbyqxmd.com/read/28606838/the-role-of-low-intensity-pulse-ultrasound-lipus-on-cartilage-healing-in-knee-osteoarthritis-a-review
#14
REVIEW
Joshua Rothenberg, Prathap Jayaram, Usker Naqvi, Joslyn Gober, Gerard A Malanga
Ultrasound (US) is a therapeutic modality that has been used in the treatment of musculoskeletal conditions for decades. In recent years there has been technological advancements using Low-Intensity Pulsed Ultrasound (LIPUS) as a clinical modality. The purpose of this review was to critically examine the medical literature to determine the effects of low-intensity pulse ultrasound (LIPUS) on the chondrogenic properties of knee osteoarthritis. A literature search of three major databases (Pubmed, Scopus, EMBASE) was performed...
June 9, 2017: PM & R: the Journal of Injury, Function, and Rehabilitation
https://www.readbyqxmd.com/read/28583325/neuroprotective-effect-of-low-intensity-pulsed-ultrasound-against-mpp-induced-neurotoxicity-in-pc12-cells-involvement-of-k2p-channels-and-stretch-activated-ion-channels
#15
Lu Zhao, Yi Feng, Aiwei Shi, Lei Zhang, Shifang Guo, Mingxi Wan
Parkinson's disease is the second most common neurodegenerative disease. It is characterized by the loss of dopaminergic neurons in the substantia nigra pars compacta. 1-Methyl-4-phenylpyridinium (MPP(+)) is a dopaminergic neuronal toxin that is widely used in constructing Parkinson's disease models in vitro. Low-intensity pulsed ultrasound (LIPUS) is a non-invasive therapeutic approach that has neuromodulation and neuroprotective effects in the central neural system; however, whether LIPUS can provide protection for dopaminergic neurons against MPP(+)-induced neurocytotoxicity remains unknown...
June 2, 2017: Ultrasound in Medicine & Biology
https://www.readbyqxmd.com/read/28576970/low-intensity-pulsed-ultrasound-lipus-promotes-cell-motility-through-vinculin-controlled-rac1-gtpase-activity
#16
Paul Atherton, Franziska Lausecker, Andrew Harrison, Christoph Ballestrem
Low Intensity Pulsed Ultrasound (LIPUS) is a therapy used clinically to promote healing. Using live cell imaging we show that LIPUS stimulation, acting through integrin-mediated cell-matrix adhesions, rapidly induces Rac activation associated with dramatic actin cytoskeleton rearrangements. Our study demonstrates that the mechanosensitive focal adhesion (FA) protein vinculin and both Focal Adhesion Kinase (FAK) and Rab5 have key roles in regulating these effects. Inhibiting the link of vinculin to the actin-cytoskeleton abolished LIPUS sensing...
June 2, 2017: Journal of Cell Science
https://www.readbyqxmd.com/read/28532896/healing-of-fracture-nonunions-treated-with-low-intensity-pulsed-ultrasound-lipus-a-systematic-review-and-meta-analysis
#17
Ross Leighton, J Tracy Watson, Peter Giannoudis, Costas Papakostidis, Andrew Harrison, R Grant Steen
INTRODUCTION: Bone fractures fail to heal and form nonunions in roughly 5% of cases, with little expectation of spontaneous healing thereafter. We present a systematic review and meta-analysis of published papers that describe nonunions treated with low-intensity pulsed ultrasound (LIPUS). METHODS: Articles in PubMed, Ovid MEDLINE, CINAHL, AMED, EMBASE, Cochrane Library, and Scopus databases were searched, using an approach recommended by the Methodological Index for Non-Randomized Studies (MINORS), with a Level of Evidence rating by two reviewers independently...
May 15, 2017: Injury
https://www.readbyqxmd.com/read/28527849/role-of-piezo-channels-in-ultrasound-stimulated-dental-stem-cells
#18
Qianhua Gao, Paul R Cooper, A Damien Walmsley, Ben A Scheven
INTRODUCTION: Piezo1 and Piezo2 are mechanosensitive membrane ion channels. We hypothesized that Piezo proteins may play a role in transducing ultrasound-associated mechanical signals and activate downstream mitogen-activated protein kinase (MAPK) signaling processes in dental cells. In this study, the expression and role of Piezo channels were investigated in dental pulp stem cells (DPSCs) and periodontal ligament stem cells (PDLSCs) after treatment with low-intensity pulsed ultrasound (LIPUS)...
May 17, 2017: Journal of Endodontics
https://www.readbyqxmd.com/read/28483020/effect-of-low-intensity-pulsed-ultrasound-on-the-mandibular-remodeling-following-inferior-alveolar-nerve-transection
#19
Bo Yang, Qingqing Wu, Liang Zhang, Yanjun Guo, Ping Gong
Objective To investigate the effects of low-intensity pulsed ultrasound (LIPUS) on the mandibular remodeling following inferior alveolar nerve transection (IANX) and to optimize the parameters of LIPUS in the treatment of nerve injury. Methods IANX was performed in male Sprague-Dawley rats. Four weeks after IANX,the effect of daily LIPUS (from day 1) on the transected inferior alveolar nerve was examined in terms of sensitivity to mechanical stimulation. Moreover,histopathologic changes of mandibles were analyzed by micro-CT,HE staining,Masson trichrome staining,and immunohistochemical staining...
April 20, 2017: Zhongguo Yi Xue Ke Xue Yuan Xue Bao. Acta Academiae Medicinae Sinicae
https://www.readbyqxmd.com/read/28463085/low-intensity-pulsed-ultrasound-reduces-periodontal-atrophy-in-occlusal-hypofunctional-teeth
#20
Yuki Kasahara, Risa Usumi-Fujita, Jun Hosomichi, Sawa Kaneko, Yuji Ishida, Naoki Shibutani, Yasuhiro Shimizu, Asuka Okito, Shuji Oishi, Yoichiro Kuma, Hiroyuki Yamaguchi, Takashi Ono
OBJECTIVE: To clarify whether low-intensity pulsed ultrasound (LIPUS) exposure has recovery effects on the hypofunctional periodontal ligament (PDL) and interradicular alveolar bone (IRAB). MATERIALS AND METHODS: Twelve-week-old male Sprague-Dawley rats were divided into three groups (n = 5 each): a normal occlusion (C) group, an occlusal hypofunction (H) group, and an occlusal hypofunction group subjected to LIPUS (HL) treatment. Hypofunctional occlusion of the maxillary first molar (M1) of the H and HL groups was induced by the bite-raising technique...
May 2, 2017: Angle Orthodontist
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