Yifeng He, Jun Shi, Hui Zhao, Yuefei Wang, Chi Zhang, Sai Han, Qizhi He, Xiaolan Li, Shangji Li, Wenjing Wang, Muhua Yi, Xiaoling Hu, Zhihua Xing, Hao Han, Yinshuang Gao, Qing Zhou, Linlin Lu, Jianfen Guo, Hui Cao, Caiping Lu, Yanqiang Hou, Dan Chen, Fengyun Yang, Ping Lei, Wen Di, Ji Qian, Yi Xia, Youzhong Zhang, Yang Deng, Jianlong Zhu, Congjian Xu
BACKGROUND: P16INK4A is a surrogate signature compensating for the specificity and/or sensitivity deficiencies of the human papillomavirus (HPV) DNA and Papanicolaou smear (Pap) co-test for detecting high-grade cervical squamous intraepithelial lesions or worse (HSIL+). However, traditional p16INK4A immunostaining is labour intensive and skill demanding, and subjective biases cannot be avoided. Herein, we created a high-throughput, quantitative diagnostic device, p16INK4A flow cytometry (FCM) and assessed its performances in cervical cancer screening and prevention...
March 2023: Clinical and Translational Medicine