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cardiomyocytes and flow cytometry

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https://www.readbyqxmd.com/read/29434067/inhibition-of-coxsackievirus-infection-in-cardiomyocytes-by-small-dsrna-targeting-its-cognate-coxsackievirus-adenovirus-receptor
#1
Mirnalini Sharma, Baijayantimala Mishra, Uma Nahar Saikia, Ajay Bahl, R K Ratho
Background & objectives: Coxsackievirus B (CVB), a member of human Enterovirus group, is the most common cause of viral myocarditis. Coxsackievirus adenovirus receptor (CAR) is identified as a key determinant for the entry of CVB in the target cells. Thus, blockade of receptor by RNA interference (RNAi) may inhibit the entry and pathogenesis of CVB in cardiac cells. The present study was aimed to determine the effect of CAR small dsRNA (siRNA) on coxsackieviral load and CAR expression in coxsackievirus-infected cardiomyocytes...
October 2017: Indian Journal of Medical Research
https://www.readbyqxmd.com/read/29420637/isolation-and-characterization-of-mesenchymal-stem-cells-from-human-fetus-heart
#2
Venkata Naga Srikanth Garikipati, Saurabh Pratap Singh, Yamuna Mohanram, Ashwani Kumar Gupta, Deepa Kapoor, Soniya Nityanand
BACKGROUND: Mesenchymal stem cells (MSCs) are promising cells for cardiovascular regenerative medicine. However, their potential may be limited, because of their restricted cardiovascular differentiation potential and decline in their number and functional characteristics with increasing donor age. We have previously shown that rat fetus heart harbors primitive MSCs and administration of these cells improved left ventricular (LV) function after ischemia/reperfusion injury in rats. To evaluate their potential as a new cell type for clinical cardiovascular cell therapy, we have undertaken this study on the isolation and characterization of human fetal cardiac MSCs (hfC-MSCs)...
2018: PloS One
https://www.readbyqxmd.com/read/29404859/serum-exosomes-attenuate-h2o2-induced-apoptosis-in-rat-h9c2-cardiomyocytes-via-erk1-2
#3
Pengfei Li, Zhuyuan Liu, Yuan Xie, Huanyu Gu, Qiying Dai, Jianhua Yao, Lei Zhou
Exosomes are small-sized vesicles that can be released from cells into the serum. Exosomes play important roles in regulating many biological processes including cell proliferation, apoptosis, cell cycle, and metabolism. However, the roles and mechanisms of plasma exosomes in the apoptosis of rat H9C2 cardiomyocytes are largely unknown. In this study, we isolated plasma exosomes as confirmed by the marker protein CD63. Using flow cytometry and western blot analysis, we found that exosomes attenuated hydrogen peroxide (H2O2)-induced apoptosis and improved survival of rat H9C2 cardiomyocytes...
February 5, 2018: Journal of Cardiovascular Translational Research
https://www.readbyqxmd.com/read/29377979/mir-147b-inhibits-cell-viability-and-promotes-apoptosis-of-rat-h9c2-cardiomyocytes-via-down-regulating-klf13-expression
#4
Mingxia Gu, Jing Wang, Yi Wang, Yanjuan Xu, Yingqiang Zhang, Weiqing Wu, Shuping Liao
Recently, microRNAs (miRNAs) have been shown to involve in the process of heart failure. This study aims to investigate the functional role of miR-147b in rat H9c2 cardiomyocytes and explore the underlying molecular mechanisms. Cell viability of H9c2 cells was detected by MTT assay. Cell apoptosis was detected by flow cytometry. Expression of miR-147b and KLF13 mRNA was detected by quantitative real-time PCR. The relationship between miR-147b and KLF13 was verified by dual-luciferase reporter assay. Protein levels were detected by western blot analysis...
January 25, 2018: Acta Biochimica et Biophysica Sinica
https://www.readbyqxmd.com/read/29339198/cd90-cardiac-fibroblasts-reduce-fibrosis-of-acute-myocardial-injury-in-rats
#5
Yuqiao Chang, Cixia Li, Yangyang Jia, Peng Chen, Yonglong Guo, Airong Li, Zhikun Guo
OBJECTIVE: To explore the differentiation tendency of CD90+ cardiac fibroblast (CFs) into cardiomyogenic cells in vitro and repair functions in acute myocardial infarction rats. METHODS: CD90+ subpopulation was sorted from rat CFs by flow cytometry. 10 μmoL/L of 5-Azacytosine (5-aza) was used to induce differentiation of CFs into cardiomyogenic cells. An acute myocardial infarction model was prepared by ligation of the rat left anterior descending coronary artery...
January 12, 2018: International Journal of Biochemistry & Cell Biology
https://www.readbyqxmd.com/read/29331652/salinomycin-induces-primary-chicken-cardiomyocytes-death-via-mitochondria-mediated-apoptosis
#6
Xiuge Gao, Yani Zheng, Xiangchun Ruan, Hui Ji, Lin Peng, Dawei Guo, Shanxiang Jiang
Salinomycin, as a polyether ionophore antibiotic, is extensively used as a feed additive against coccidiosis in poultry and as a growth promoter of ruminants worldwide. Owing to its narrow therapeutic index, numerous intoxication have been reported in target/non-target animals by overdosage, misuse or drug interactions as well as human who consumed salinomycin accidently. Salinomycin-induced cardiotoxicity in chicken and non-target animals is considered as a major contributor to animal death. In the current study, we aim to elucidate the underlying mechanism of its myocardial toxicity using primary chicken myocardial cell as an in vitro model...
January 10, 2018: Chemico-biological Interactions
https://www.readbyqxmd.com/read/29324310/25-hydroxyl-protopanaxatriol-protects-against-h2o2-induced-h9c2-cardiomyocytes-injury-via-pi3k-akt-pathway-and-apoptotic-protein-down-regulation
#7
Zhihao Wang, Guangyue Su, Zhiguo Zhang, Han Dong, Yuehui Wang, Huiying Zhao, Yuqing Zhao, Qi Sun
Oxidative stress injury and apoptosis are the main mechanisms of myocardial ischemia-reperfusion injury (MI/RI). Compounds with anti-oxidant properties can treat MI/RI. Therefore, identification of natural antioxidants such as herbs or botanical drugs is an ideal strategy to develop safe and effective anti-MI/RI drugs. Cardioprotective effects of Ginseng are well documented and are attributable to its anti-oxidant, anti-inflammatory, anti-tumorigenic, anti-arrhythmic, anti-ischemic properties. Ginseng monomer 20®-dammarane -3 beta,6 alpha,12 beta,20,25-pentol(25-hydroxyl-Protopanaxatriol,25-OH-PPT), a novel compound, which belongs to panaxatriol category, is extracted from the leaves and stem of ginseng...
January 8, 2018: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://www.readbyqxmd.com/read/29296972/bone-marrow-transplantation-prevents-right-ventricle-disease-in-the-caveolin-1-deficient-mouse-model-of-pulmonary-hypertension
#8
Kewal Asosingh, Nicholas Wanner, Kelly Weiss, Kimberly Queisser, Liya Gebreab, Biruk Kassa, Eric Stuehr, Brian Graham, Serpil Erzurum
Accumulating evidence shows a causative role for the bone marrow (BM) in the genesis and progression of pulmonary hypertension (PH). Engraftment of BM hematopoietic stem cells from PH patients to mice reproduces the cardiopulmonary pathology of PH. However, it is unknown whether healthy BM can prevent the development of right heart disease. Caveolin-1-deficient (CAV-1 KO) mice develop cardiopulmonary disease with manifestations resembling PH, including elevated right ventricular (RV) systolic pressure (RVSP), RV hypertrophy, and pulmonary endothelial proliferative disease...
March 28, 2017: Blood Advances
https://www.readbyqxmd.com/read/29286090/therapeutic-inhibition-of-galectin%C3%A2-3-improves-cardiomyocyte-apoptosis-and-survival-during-heart-failure
#9
Xia Li, Xuan Tang, Jinping Lu, Sheng Yuan
Galectin-3 is an important mediator of cardiac fibrosis and heart failure. Cell viability of cardiomyocytes was measured using a CCK‑8 assay; flow cytometry was employed for the detection of the cell cycle and cardiomyocytes apoptosis. Reverse transcription‑quantitative polymerase chain reaction and western blotting was performed to examine the expression of associated genes and proteins. The present study demonstrated that overexpression of galectin‑3 significantly decreased the viability of cardiomyocytes in a time‑dependent manner, with simultaneous arrest of the cell cycle and induction of apoptosis...
December 20, 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/29243790/mir-93-inhibition-ameliorates-ogd-r-induced-cardiomyocyte-apoptosis-by-targeting-nrf2
#10
L-J Yan, X-W Fan, H-T Yang, J-T Wu, S-L Wang, C-G Qiu
OBJECTIVE: To identify the expression changes of microRNA 93 (miR-93) in oxygen-glucose deprivation/reoxygenation (OGD/R) injury in cardiomyocytes and its mechanism of mediating OGD/R and inducing apoptosis. MATERIALS AND METHODS: Primary cardiomyocytes were extracted and OGD/R model in cardiomyocytes was established in vitro. Quantitative reverse transcription polymerase chain reaction (qRT-PCR) was used to detect the expressions of miR-93, and Western blot assay was applied to measure the protein levels of nuclear factor erythroid 2-related factor 2 (Nrf2) and caspase-3...
December 2017: European Review for Medical and Pharmacological Sciences
https://www.readbyqxmd.com/read/29238517/exogenous-h2s-restores-ischemic-post-conditioning-induced-cardioprotection-through-inhibiting-endoplasmic-reticulum-stress-in-the-aged-cardiomyocytes
#11
Weiming Sun, Jinxia Yang, Yuanzhou Zhang, Yuxin Xi, Xin Wen, Di Yuan, Yuehong Wang, Can Wei, Rui Wang, Lingyun Wu, Hongzhu Li, Changqing Xu
Background: A gasotransmitter hydrogen sulfide (H2S) plays an important physiological and pathological role in cardiovascular system. Ischemic post-conditioning (PC) provides cardioprotection in the young hearts but not in the aged hearts. Exogenous H2S restores PC-induced cardioprotection by inhibition of mitochondrial permeability transition pore opening and oxidative stress and increase of autophagy in the aged hearts. However, whether H2S contributes to the recovery of PC-induced cardioprotection via down-regulation of endoplasmic reticulum stress (ERS) in the aged hearts is unclear...
2017: Cell & Bioscience
https://www.readbyqxmd.com/read/29221138/gas-signaling-molecule-hydrogen-sulfide-attenuates-doxorubicin-induced-dilated-cardiomyopathy
#12
Zongliang Yu, Wei Zhang, Mengyao Zhang, Mengchao Jin, Weiting Xu, Xiang Zhou
Increasing evidence has revealed that hydrogen sulfide (H2S) has beneficial effects in the treatment of various cardiovascular diseases. However, whether H2S can attenuate the development of dilated cardiomyopathy (DCM) remains unclear. In this study, we generated a rat model of DCM induced by doxorubicin and investigated the protective effects of H2S against DCM. Cardiac structure and function were analyzed by two-dimensional echocardiography. Oxidative stress was evaluated by measuring malondialdehyde, superoxide dismutase, glutathione peroxidase and reactive oxygen species...
November 10, 2017: Oncotarget
https://www.readbyqxmd.com/read/29216265/oct4-expression-mediates-partial-cardiomyocyte-reprogramming-of-mesenchymal-stromal-cells
#13
Gustavo Yannarelli, Natalia Pacienza, Sonia Montanari, Diego Santa-Cruz, Sowmya Viswanathan, Armand Keating
Mesenchymal stem/stromal cells (MSCs) are in numerous cell therapy clinical trials, including for injured myocardium. Acquisition of cardiomyocyte characteristics by MSCs may improve cardiac regeneration but the mechanisms regulating this process are unclear. Here, we investigated whether the pluripotency transcription factor OCT4 is involved in the activation of cardiac lineage genetic programs in MSCs. We employed our established co-culture model of MSCs with rat embryonic cardiomyocytes showing co-expression of cardiac markers on MSCs independent of cell fusion...
2017: PloS One
https://www.readbyqxmd.com/read/29208259/the-role-of-a-novel-long-noncoding-rna-tuc40-in-cardiomyocyte-induction-and-maturation-in-p19-cells
#14
Huijuan Li, Li Jiang, Zhangbin Yu, Shuping Han, Xuehua Liu, Mengmeng Li, Chun Zhu, Lixing Qiao, Li Huang
BACKGROUND: In previous studies, TUC40-, a new long noncoding RNA, was found to be overexpressed in human ventricular septal defect (VSD) embryonic heart samples. In this article, we carried out experiments on the P19 cell line to elucidate the effects of TUC40- overexpression on cardiomyocyte development relevant to VSD pathogenesis. METHODS: We established the overexpression cell model by plasmid transfection, and explored the expression profile of Pbx1, the sense gene of TUC40-, and the marker genes of cardiomyocyte linage commitment (Nkx2...
December 2017: American Journal of the Medical Sciences
https://www.readbyqxmd.com/read/29179205/overexpression-of-timp3-protects-against-cardiac-ischemia-reperfusion-injury-by-inhibiting-myocardial-apoptosis-through-ros-mapks-pathway
#15
Hui Liu, Xibo Jing, Aiqiao Dong, Baobao Bai, Haiyan Wang
BACKGROUND/AIMS: Myocardial ischemia/reperfusion (I/R) injury remains a great challenge in clinical therapy. Tissue inhibitor of metalloproteinases 3 (TIMP3) plays a crucial role in heart physiological and pathophysiological processes. However, the effects of TIMP3 on I/R injury remain unknown. METHODS: C57BL/6 mice were infected with TIMP3 adenovirus by local delivery in myocardium followed by I/R operation or doxorubicin treatment. Neonatal rat cardiomyocytes were pretreated with TIMP3 adenovirus prior to anoxia/reoxygenation (A/R) treatment in vitro...
November 27, 2017: Cellular Physiology and Biochemistry
https://www.readbyqxmd.com/read/29179175/novel-functional-role-of-heat-shock-protein-90-in-mitochondrial-connexin-43-mediated-hypoxic-postconditioning
#16
Rong-Hui Tu, Qing-Jie Li, Zheng Huang, Yan He, Jian-Jun Meng, Hui-Lei Zheng, Zhi-Yu Zeng, Guo-Qiang Zhong
BACKGROUND/AIMS: Previous studies have shown that heat shock protein 90 (HSP90)-mediated mitochondrial import of connexin 43 (Cx43) is critical in preconditioning cardioprotection. The present study was designed to test whether postconditioning has the same effect as preconditioning in promoting Cx43 translocation to mitochondria and whether mitochondrial HSP90 modulates this effect. METHODS: Cellular models of hypoxic postconditioning (HPC) from rat heart-derived H9c2 cells and neonatal rat cardiomyocytes were employed...
November 27, 2017: Cellular Physiology and Biochemistry
https://www.readbyqxmd.com/read/29175072/capsaicin-prevents-mitochondrial-damage-protects-cardiomyocytes-subjected-to-anoxia-reoxygenation-injury-mediated-by-14-3-3%C3%AE-bcl-2
#17
Jiyi Huang, Zhantu Liu, Ping Xu, Zeyu Zhang, Dong Yin, Jichun Liu, Huan He, Ming He
Capsaicin(Cap) is an active component of chili peppers that is extracted from capsicum plants. Recent studies have reported that Cap can ameliorate myocardial ischemia/reperfusion(I/R) injury. Mitochondria play an important role in pathways of apoptosis induced by myocardial I/R injury. However, the underlying mechanisms of Cap that improve mitochondrial function during I/R injury is not yet understood. The aim of this study was to evaluate whether Cap regained normal mitochondrial function in myocardial I/R injury, and to further explore the underlying mechanisms of action involved...
November 21, 2017: European Journal of Pharmacology
https://www.readbyqxmd.com/read/29166707/-establishment-of-myocardial-targeted-nanoparticles-and-preliminary-evaluation-of-its-effects-on-prevention-and-treatment-of-myocardial-injury
#18
Y Y Liu, C Wang, P F Luo, Z F Xia
Objective: To establish 3-{4-[2-hydroxyl-(1-methylethylamino) propoxy] phenyl} propionic acid cetylesters (PAC) modified nanoparticles, and preliminarily explore its cardiomyocyte-targeting function and protection effects on myocardium. Methods: (1) HL-1 myocardial cells were divided into cyanidin-3 (Cy3) marked non-targeted small interference RNA (Cy3-siNC) group and Cy3 marked small interference RNA designed for the nuclear factor kappa B (NF-κB)-p65 gene (Cy3-si435) group according to the random number table, with 3 wells in each group...
November 20, 2017: Zhonghua Shao Shang za Zhi, Zhonghua Shaoshang Zazhi, Chinese Journal of Burns
https://www.readbyqxmd.com/read/29115535/jujuboside-a-attenuates-norepinephrine-induced-apoptosis-of-h9c2-cardiomyocytes-by-modulating-mapk-and-akt-signaling-pathways
#19
Chang-Rong Wan, Dan-Dan Han, Jian-Qin Xu, Peng Yin, Xiao-Long Xu, Chen Mei, Feng-Hua Liu, Zhao-Fei Xia
Cardiomyocyte apoptosis is closely associated with the pathogenesis of heart failure. Jujuboside A (JUA) is a type of saponin isolated from the seeds of Zizyphus jujuba. In traditional Chinese medicine, it is believed that JUA possesses multiple biological effects, including antianxiety, antioxidant and anti‑inflammatory activities. The present study aimed to evaluate the effects of JUA on norepinephrine (NE)‑induced apoptosis of H9c2 cells and to investigate its underlying mechanisms. Rat H9c2 cardiomyocytes were pretreated with JUA and were then exposed to NE as an in vitro model of myocardial apoptosis...
November 2, 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/29115425/the-hmgb1%C3%A2-il%C3%A2-17a-axis-contributes-to-hypoxia-reoxygenation-injury-via-regulation-of-cardiomyocyte-apoptosis-and-autophagy
#20
Xiaorong Hu, Kai Zhang, Zhiqiang Chen, Hong Jiang, Weipan Xu
Both the high‑mobility group box 1 protein (HMGB1) and interleukin (IL)‑17A serve important roles in myocardial ischemia and reperfusion injury. The purpose of the present study was to evaluate whether HMGB1 could induce IL‑17A secretion and lead to cardiomyocyte hypoxia/reoxygenation (H/R) injury. Neonatal rat cardiomyocytes were treated with HMGB1‑neutralizing antibody, IL‑17A‑neutralizing antibody, recombinant HMGB1 (rHMGB1) and recombinant IL‑17A (rIL‑17A), respectively. Cell viabilities, lactate dehydrogenase and creatine kinase levels were measured...
October 20, 2017: Molecular Medicine Reports
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