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https://www.readbyqxmd.com/read/29240284/extracellular-matrix-stromal-vascular-fraction-gel-conditioned-medium-accelerates-wound-healing-in-a-murine-model
#1
Chengliang Deng, Yunfan He, Jingwei Feng, Ziqing Dong, Yao Yao, Hsiaopei Mok, Maohui Lin, Lu Feng
Conditioned medium (CM) is a new treatment modality in regenerative medicine and has shown a successful outcome in wound healing. We recently introduced extracellular matrix/stromal vascular fraction gel (ECM/SVF-gel), an adipose-derived stem cell and adipose native extracellular matrix-enriched product for cytotherapy. This study aimed to evaluate the effect of CM from ECM/SVF-gel (Gel-CM) on wound healing compared with the conventional CM from adipose tissue (Adi-CM) and stem cell (SVF-CM). In vitro wound healing effect of three CMs on keratinocytes and fibroblasts was evaluated in terms of proliferation property, migratory property, and extracellular matrix production...
December 14, 2017: Wound Repair and Regeneration
https://www.readbyqxmd.com/read/29239730/directed-differentiation-of-periocular-mesenchyme-from-human-embryonic-stem-cells
#2
Matthew Lovatt, Gary Hin-Fai Yam, Gary S Peh, Alan Colman, N Ray Dunn, Jodhbir S Mehta
Corneal tissue is the most transplanted of all body tissues. Currently, cadaveric donor tissues are used for transplantation. However, a global shortage of transplant grade material has prompted development of alternative, cell-based therapies for corneal diseases. Pluripotent stem cells are attractive sources of cells for regenerative medicine, because large numbers of therapeutically useful cells can be generated. However, a detailed understanding of how to differentiate clinically relevant cell types from stem cells is fundamentally required...
November 16, 2017: Differentiation; Research in Biological Diversity
https://www.readbyqxmd.com/read/29239062/gvhd-amelioration-by-human-bone-marrow-mesenchymal-stromal-stem-cell-derived-extracellular-vesicles-is-associated-with-peripheral-preservation-of-naive-t-cell-populations
#3
Sumie Fujii, Yasuo Miura, Aya Fujishiro, Takero Shindo, Yutaka Shimazu, Hideyo Hirai, Hidetoshi Tahara, Akifumi Takaori-Kondo, Tatsuo Ichinohe, Taira Maekawa
A substantial proportion of patients with acute graft-versus-host disease (aGVHD) respond to cell therapy with culture-expanded human bone marrow mesenchymal stromal/stem cells (BM-MSCs). However, the mechanisms by which these cells can ameliorate aGVHD-associated complications remain to be clarified. We show here that BM-MSC-derived extracellular vesicles (EVs) recapitulated the therapeutic effects of BM-MSCs against aGVHD. Systemic infusion of human BM-MSC-derived EVs prolonged the survival of mice with aGVHD and reduced the pathologic damage in multiple GVHD-targeted organs...
December 14, 2017: Stem Cells
https://www.readbyqxmd.com/read/29236575/comparing-different-nanofat-procedures-on-scars-role-of-the-stromal-vascular-fraction-and-its-clinical-implications
#4
Pietro Gentile, Giovanna Maria Scioli, Alessandra Bielli, Augusto Orlandi, Valerio Cervelli
AIM: Recently, a new fat grafting technique termed 'nanofat grafting' was proposed which improved tissue repair by the stem cells contained in the stromal vascular fraction (SVF) of nanofat. Here, we reported the clinical outcomes of different nanofat procedures in the treatment of scars in relation with SVF cell yield. METHODS: Three different modified nanofat grafting procedures (supercharged-, evo- and centrifuged-modified nanofat) were compared with the classic nanofat method, and histological analysis was performed to assess skin regeneration...
December 13, 2017: Regenerative Medicine
https://www.readbyqxmd.com/read/29236198/sdf-1-induces-tnf-mediated-apoptosis-in-cardiac-myocytes
#5
Andrew A Jarrah, Martina Schwarskopf, Edward R Wang, Thomas LaRocca, Ashwini Dhume, Shihong Zhang, Lahouria Hadri, Roger J Hajjar, Alison D Schecter, Sima T Tarzami
Chemokines are small secreted proteins with chemoattractant properties that play a key role in inflammation. One such chemokine, Stromal cell-derived factor-1 (SDF-1) also known as CXCL12, and its receptor, CXCR4, are expressed and functional in cardiac myocytes. SDF-1 both stimulates and enhances the cellular signal which attracts potentially beneficial stem cells for tissue repair within the ischemic heart. Paradoxically however, this chemokine is known to act in concert with the inflammatory cytokines of the innate immune response which contributes to cellular injury through the recruitment of inflammatory cells during ischemia...
December 13, 2017: Apoptosis: An International Journal on Programmed Cell Death
https://www.readbyqxmd.com/read/29235199/adaptation-of-the-bone-marrow-stroma-in-hematopoietic-malignancies-current-concepts-and-models
#6
REVIEW
Ben Doron, Mithila Handu, Peter Kurre
The bone marrow stroma maintains hematopoiesis and coordinately regulates regenerative responses through dynamic interactions with hematopoietic stem and progenitor cells. Recent studies indicate that stromal components in the bone marrow of leukemia patients undergo a process of successive adaptation that in turn exerts dramatic effects on the hematopoietic stem cell compartment and promotes leukemic drug resistance. Functional changes in discrete marrow stromal populations can therefore be considered an aspect of leukemia biogenesis in that they create an aberrant, self-reinforcing microenvironment...
December 13, 2017: Stem Cells
https://www.readbyqxmd.com/read/29235086/endothelial-expression-of-c-kit-and-cd68-in-dental-follicles-of-human-impacted-third-molars
#7
Mugurel Constantin Rusu, Aliona Rașcu, Mihai Dragomir Stoenescu
Periodontal tissue remnants of odontogenesis constitute the dental follicle (DF) which is actually considered a stem niche in adults. However, potentialities of local endothelia within this niche seem overlooked. We thus aimed at testing the endothelial cells expression of c-kit, the progenitor cells marker, and CD68, commonly regarded as a monocyte/macrophage marker, in human DFs. We performed an immunohistochemical study using these two markers which were applied on samples collected from ten adult patients...
December 13, 2017: Folia Morphologica (Warsz)
https://www.readbyqxmd.com/read/29234689/data-supporting-that-adipose-derived-mesenchymal-stem-stromal-cells-express-angiotensin-ii-receptors-in-situ-and-in-vitro
#8
Liudmila V Ageeva, Veronika Y Sysoeva, Pyotr A Tyurin-Kuzmin, George V Sharonov, Daniyar T Dyikanov, Dmitry V Stambolsky, Natalia I Kalinina
This article contains results of analyses of angiotensin II receptors expression in human adipose tissue and stem/stromal cells isolated from adipose tissue. We also provide here data regarding the effect of angiotensin II on intracellular calcium mobilization in adipose tissue derived stem/stromal cells (ADSCs). Discussion of the data can be found in (Sysoeva et al., 2017) [1].
February 2018: Data in Brief
https://www.readbyqxmd.com/read/29233979/extracellular-vesicles-from-human-induced-pluripotent-stem-cell-derived-mesenchymal-stromal-cells-hipsc-mscs-protect-against-renal-ischemia-reperfusion-injury-via-delivering-specificity-protein-sp1-and-transcriptional-activating-of-sphingosine-kinase-1-and
#9
Xiaodong Yuan, Dawei Li, Xiaosong Chen, Conghui Han, Longmei Xu, Tao Huang, Zhen Dong, Ming Zhang
Renal ischemia-reperfusion is a main cause of acute kidney injury (AKI), which is associated with high mortality. Here we show that extracellular vesicles (EVs) secreted from hiPSC-MSCs play a critical role in protection against renal I/R injury. hiPSC-MSCs-EVs can fuse with renal cells and deliver SP1 into target cells, subsequently active SK1 expression and increase S1P formation. Chromatin immunoprecipitation (ChIP) analyses and luciferase assay were used to confirm SP1 binds directly to the SK1 promoter region and promote promoter activity...
December 11, 2017: Cell Death & Disease
https://www.readbyqxmd.com/read/29231967/therapeutic-potential-of-porcine-bronchoalveolar-fluid-derived-mesenchymal-stromal-cells-in-a-pig-model-of-lps-induced-ali
#10
Mahesh Khatri, Levi Arthur Richardson
In this study, we isolated mesenchymal stromal (stem) cells (MSCs) from broncho-alveolar lavage fluid (BAL) of 2-6 week-old commercial pigs. BAL-MSCs displayed fibroblastic morphology and possessed self-renewal properties. Similar to bone-marrow MSCs, BAL-MSCs expressed mesenchymal markers and both cell types lacked the expression of hematopoetic markers. BAL-MSCs, when cultured in differentiation induction media, differentiated into adipocytes, osteocytes and chondrocytes. Next, we examined if BAL-MSCs have the ability to treat lipopolysaccharide (LPS)-induced acute lung injury (ALI) in a pig model...
December 12, 2017: Journal of Cellular Physiology
https://www.readbyqxmd.com/read/29230885/intercellular-transfer-of-microvesicles-from-young-mesenchymal-stromal-cells-rejuvenates-aged-murine-hematopoietic-stem-cells
#11
Rohan Kulkarni, Manmohan Bajaj, Suprita Ghode, Sapana Jalnapurkar, Lalita Limaye, Vaijayanti Kale
Donor age is one of the major concerns in Bone Marrow Transplantation, as the aged hematopoietic stem cells (HSCs) fail to engraft efficiently. Here, using murine system we show that a brief interaction of aged HSCs with young mesenchymal stromal cells (MSCs) rejuvenates them and restores their functionality via inter-cellular transfer of microvesicles (MVs) containing autophagy-related mRNAs. Importantly, we show that MSCs gain activated AKT signaling as a function of aging. Activated AKT reduces the levels of autophagy-related mRNAs in their MVs, and partitions miR-17 and miR-34a into their exosomes, which upon transfer into HSCs down-regulate their autophagy-inducing mRNAs...
December 12, 2017: Stem Cells
https://www.readbyqxmd.com/read/29230389/cyclic-tensile-strain-can-play-a-role-in-directing-both-intramembranous-and-endochondral-ossification-of-mesenchymal-stem-cells
#12
Simon F Carroll, Conor T Buckley, Daniel J Kelly
Successfully regenerating damaged or diseased bone and other joint tissues will require a detailed understanding of how joint specific environmental cues regulate the fate of progenitor cells that are recruited or delivered to the site of injury. The goal of this study was to explore the role of cyclic tensile strain (CTS) in regulating the initiation of mesenchymal stem cell/multipotent stromal cell (MSC) differentiation, and specifically their progression along the endochondral pathway. To this end, we first explored the influence of CTS on the differentiation of MSCs in the absence of any specific growth factor, and secondly, we examined the influence of the long-term application of this mechanical stimulus on markers of endochondral ossification in MSCs maintained in chondrogenic culture conditions...
2017: Frontiers in Bioengineering and Biotechnology
https://www.readbyqxmd.com/read/29230039/gli1-identifies-osteogenic-progenitors-for-bone-formation-and-fracture-repair
#13
Yu Shi, Guangxu He, Wen-Chih Lee, Jennifer A McKenzie, Matthew J Silva, Fanxin Long
Bone formation in mammals requires continuous production of osteoblasts throughout life. A common molecular marker for all osteogenic mesenchymal progenitors has not been identified. Here, by lineage-tracing experiments in fetal or postnatal mice, we discover that Gli1+ cells progressively produce osteoblasts in all skeletal sites. Most notably, in postnatal growing mice, the Gli1+ cells residing immediately beneath the growth plate, termed here "metaphyseal mesenchymal progenitors" (MMPs), are essential for cancellous bone formation...
December 11, 2017: Nature Communications
https://www.readbyqxmd.com/read/29228630/transplantation-of-ips-cell-derived-neural-progenitors-overexpressing-sdf-1%C3%AE-increases-regeneration-and-functional-recovery-after-ischemic-stroke
#14
Monica Chau, Todd C Deveau, Mingke Song, Zheng Z Wei, Xiaohuan Gu, Shan Ping Yu, Ling Wei
Ischemic stroke is a leading cause of human death and disability while clinical treatments are limited. The adult brain possesses endogenous regenerative activities that may benefit tissue repair after stroke. Trophic factors such as stromal cell-derived factor 1 alpha (SDF-1α) are upregulated in the ischemic brain, which promote endogenous regeneration. The regenerative response, however, is normally insufficient. Transplantation of exogenous cells has been explored as regenerative therapies. One promising cell type for transplantation is induced pluripotent stem (iPS) cells which are cells genetically reprogrammed from adult somatic cells...
November 14, 2017: Oncotarget
https://www.readbyqxmd.com/read/29227527/m2-muscarinic-receptor-activation-inhibits-cell-proliferation-and-migration-of-rat-adipose-mesenchymal-stem-cells
#15
Roberta Piovesana, Simona Melfi, Mario Fiore, Valerio Yi, Ada Maria Tata
Mesenchymal stem cells (MSCs), also known as stromal mesenchymal stem cells, are multipotent cells, which can be found in many tissues and organs as bone marrow, adipose tissue and other tissues. In particular MSCs derived from Adipose tissue (ADSCs) are the most frequently used in regenerative medicine because they are easy to source, rapidly expandable in culture and excellent differentiation potential into adipocytes, chondrocytes and other cell types. Acetylcholine (ACh), is one of the most important neurotransmitter in central (CNS) and peripheral nervous system (PNS), playing important roles also in non-neural tissue, but its functions in MSCs are still not investigated...
December 11, 2017: Journal of Cellular Physiology
https://www.readbyqxmd.com/read/29227245/clearance-of-senescent-decidual-cells-by-uterine-natural-killer-cells-in-cycling-human-endometrium
#16
Paul J Brighton, Yojiro Maruyama, Katherine Fishwick, Pavle Vrljicak, Shreeya Tewary, Risa Fujihara, Joanne Muter, Emma S Lucas, Taihei Yamada, Laura Woods, Raffaella Lucciola, Yie Hou Lee, Satoru Takeda, Sascha Ott, Myriam Hemberger, Siobhan Quenby, Jan Joris Brosens
In cycling human endometrium, menstruation is followed by rapid estrogen-dependent growth. Upon ovulation, progesterone and rising cellular cAMP levels activate the transcription factor Forkhead box O1 (FOXO1) in endometrial stromal cells (EnSCs), leading to cell cycle exit and differentiation into decidual cells that control embryo implantation. Here we show that FOXO1 also causes acute senescence of a subpopulation of decidualizing EnSCs in an IL-8 dependent manner. Selective depletion or enrichment of this subpopulation revealed that decidual senescence drives the transient inflammatory response associated with endometrial receptivity...
December 11, 2017: ELife
https://www.readbyqxmd.com/read/29225601/anti-donor-immune-responses-elicited-by-allogeneic-mesenchymal-stem-cells-and-their-extracellular-vesicles-are-we-still-learning
#17
REVIEW
Paul Lohan, Oliver Treacy, Matthew D Griffin, Thomas Ritter, Aideen E Ryan
Mesenchymal stromal cells (MSC) have been used to treat a broad range of disease indications such as acute and chronic inflammatory disorders, autoimmune diseases, and transplant rejection due to their potent immunosuppressive/anti-inflammatory properties. The breadth of their usage is due in no small part to the vast quantity of published studies showing their ability to modulate multiple immune cell types of both the innate and adaptive immune response. While patient-derived (autologous) MSC may be the safer choice in terms of avoiding unwanted immune responses, factors including donor comorbidities may preclude these cells from use...
2017: Frontiers in Immunology
https://www.readbyqxmd.com/read/29225483/active-stromal-cell-derived-factor-1%C3%AE-and-endothelial-progenitor-cells-are-equally-increased-by-alogliptin-in-good-and-poor-diabetes-control
#18
Roberto Negro, Eupremio Luigi Greco, Giacomo Greco
Background: It is postulated that the ability of dipeptidyl peptidase-4 inhibitors (DPP-4-i) to increase circulating endothelial progenitor cells (EPCs) may be at least partly mediated by active stromal cell-derived factor 1α (SDF-1α) (a pivotal mediator of stem cell mobilization from the bone marrow). As other DPP-4-i were demonstrated to increase EPC concentrations, in this study, we sought to investigate the ability of the DPP-4-i alogliptin in modifying EPCs and SDF-1α, in patients with good and poor diabetes control...
2017: Clinical Medicine Insights. Endocrinology and Diabetes
https://www.readbyqxmd.com/read/29225457/corneal-regeneration-by-induced-human-buccal-mucosa-cultivated-on-an-amniotic-membrane-following-alkaline-injury
#19
Rohaina Che Man, Then Kong Yong, Ng Min Hwei, Wan Haslina Wan Abdul Halim, Aida Zairani Mohd Zahidin, Roszalina Ramli, Aminuddin Bin Saim, Ruszymah Binti Hj Idrus
Various clinical disorders and injuries, such as chemical, thermal, or mechanical injuries, may lead to corneal loss that results in blindness. PURPOSE: The aims of this study were to differentiate human buccal mucosa (BMuc) into corneal epithelial-like cells, to fabricate engineered corneal tissue using buccal mucosal epithelial cells, and to reconstruct a damaged corneal epithelium in a nude rat model. Methods: BMuc were subjected to 10 d of induction factors to investigate the potential of cells to differentiate into corneal lineages...
2017: Molecular Vision
https://www.readbyqxmd.com/read/29222844/effect-of-age-and-body-mass-index-on-the-yield-of-stromal-vascular-fraction
#20
Nada Alaaeddine, Oula El Atat, Nagib Saliba, Ziad Feghali, Marwan Nasr, Bilal Tarabey, George Hilal, Hussein Hashim
OBJECTIVE: The aim of this study was to determine the influence of age, body mass index, and site of liposuction on the cell yield of SVF. METHODS: A prospective study was performed on 58 patients. The average age was 39 years old, with BMI ≤ 25 or BMI ≥ 25. Fat tissue was harvested from the abdominal region, flanks, or thighs and SVF was isolated. RESULTS: The yield of viable SVF was evaluated by trypan blue, and the markers of stem cells were evaluated by flow cytometry...
December 8, 2017: Journal of Cosmetic Dermatology
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