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stem cell stromal

Juliane Ertl, Melanie Pichlsberger, Alexandru-Cristian Tuca, Paul Wurzer, Jakob Fuchs, Stefan H Geyer, Barbara Maurer-Gesek, Wolfgang J Weninger, Dagmar Pfeiffer, Vladimir Bubalo, Daryousch Parvizi, Lars-Peter Kamolz, Ingrid Lang
OBJECTIVE: Mesenchymal stem/stromal cells derived from human term placentas (PMSCs) are novel therapeutic agents and more topical than ever. Here we evaluated the effects of three types of PMSCs on wound healing in an in vivo mouse model: Amnion-derived MSCs (AMSCs), blood vessel-derived MSCs (BV-MSCs) from the chorionic plate and Wharton's jelly-derived MSCs (WJ-MSCs) from the umbilical cord. METHODS: We topically applied PMSCs onto skin wounds in mice using the dermal substitute Matriderm® as carrier and evaluated wound healing parameters...
May 2018: Placenta
Martina Rossi, Barbara Roda, Silvia Zia, Ilaria Vigliotta, Chiara Zannini, Francesco Alviano, Laura Bonsi, Andrea Zattoni, Pierluigi Reschiglian, Alessandro Gennai
Background: New microfat preparations provide material suitable for use as a regenerative filler for different facial areas. To support the development of new robust techniques for regenerative purposes, the cellular content of the sample should be considered. Objectives: To evaluate the stromal vascular fraction (SVF) cell components of micro-superficial enhanced fluid fat injection (SEFFI) samples via a technique to harvest re-injectable tissue with minimum manipulation...
June 14, 2018: Aesthetic Surgery Journal
Jiping Sun, Jing Lv, Wenjing Zhang, Lili Li, Jia Lv, Yingzhou Geng, Aiping Yin
This in vitro study was performed to identify the role of miR-124a in bone marrow stromal stem cells (BMSCs) therapy for H2 O2 -induced rat podocyte injury, and determine whether combination treatment with miR-124a could improve the protective effect of BMSCs. Cell viability of podocytes was detected by CCK-8 assay. Detection of ROS level, apoptotic rate, and autophagy rate was carried out using flow cytometry assays. Oxidative stress parameters were analyzed using the ELISA assays. MiR-124a and mRNA levels were determined using real-time PCR...
June 15, 2018: Journal of Cellular Biochemistry
Nicola Alessio, Caterina Pipino, Domitilla Mandatori, Pamela Di Tomo, Angela Ferone, Marco Marchiso, Mariarosa A B Melone, Gianfranco Peluso, Assunta Pandolfi, Umberto Galderisi
Mesenchymal stromal cells (MSCs) are considered to be an excellent source in regenerative medicine. They contain several cell subtypes, including multipotent stem cells. MSCs are of particular interest as they are currently being tested using cell and gene therapies for a number of human diseases. They represent a rare population in tissues; for this reason, they require, before being transplanted, an in vitro amplification. This process may induce replicative senescence, thus affecting differentiation and proliferative capacities...
June 15, 2018: Journal of Cellular Physiology
Yang Lv, Bo Liu, Yuan Liu, Haoyu Wang, Haiping Wang
Transforming growth factor β1 (TGF-β1) and salvianolic acid B (Sal-B) are key signaling factors for stem cell differentiation into cardiomyocytes (CMs). The present study compared the biological effect of TGF-β1 and Sal-B, alone or in combination, on bone marrow mesenchymal stromal cells (BMSCs) that differentiate into myocardial-like cells in a simulated myocardial microenvironment in vitro . BMSCs were isolated from bones of limbs of 10 male Sprague Dawley rats and cultured. The 2nd-generation BMSCs were co-incubated with TGF-β1 and Sal-B, alone or in combination, for 72 h...
June 2018: Experimental and Therapeutic Medicine
Xingbing Wang, Xu Ye, Jingjuan Ji, Jian Wang, Bo Xu, Qian Zhang, Jing Ming, Xin Liu
Toll‑like receptors (TLRs) are expressed in human bone marrow‑derived mesenchymal stromal cells (BM‑MSCs). The activation of TLRs is important in the proliferation, differ-entiation, migration and hematopoiesis‑supporting functions of BM‑MSCs. MicroRNAs (miRNAs) are involved in various biological functions by mediating mRNA degradation or inhibiting the translation of target genes. Our previous study confirmed that TLRs regulate the migration ability of BM‑MSCs. It was also identified that multiple miRNAs were regulated by TLRs...
June 5, 2018: International Journal of Molecular Medicine
Rongyao Xu, Xiang Shen, Yameng Si, Yu Fu, Weiwen Zhu, Tao Xiao, Zongyun Fu, Ping Zhang, Jie Cheng, Hongbing Jiang
The alteration of age-related molecules in the bone marrow microenvironment is one of the driving forces in osteoporosis. These molecules inhibit bone formation and promote bone resorption by regulating osteoblastic and osteoclastic activity, contributing to age-related bone loss. Here, we observed that the level of microRNA-31a-5p (miR-31a-5p) was significantly increased in bone marrow stromal cells (BMSCs) from aged rats, and these BMSCs demonstrated increased adipogenesis and aging phenotypes as well as decreased osteogenesis and stemness...
June 12, 2018: Aging Cell
Yu Zhang, Hongxia Zhang, Siyang Lin, Xudong Chen, Yu Yao, XiaoOu Mao, Bei Shao, Qichuan Zhuge, Kunlin Jin
Stromal-derived factor-1 (SDF-1, also known as CXCL12) and its receptors CXCR4 and CXCR7 play important roles in brain repair after ischemic stroke, as SDF-1/ CXCR4/CXCR7 chemokine signaling is critical for recruiting stem cells to sites of ischemic injury. Upregulation of SDF-1/CXCR4/CXCR7 chemokine signaling in the ischemic regions has been well-documented in the animal models of ischemic stroke, but not in human ischemic brain. Here, we found that protein expression of SDF-1 and CXCR7, but not CXCR4, were significantly increased in the cortical peri-infarct regions (penumbra) after ischemic stroke in human, compared with adjacent normal tissues and control subjects...
April 2018: Aging and Disease
Yufang Shi, Yu Wang, Qing Li, Keli Liu, Jianquan Hou, Changshun Shao, Ying Wang
Mesenchymal stem cells (MSCs; also referred to as mesenchymal stromal cells) have attracted much attention for their ability to regulate inflammatory processes. Their therapeutic potential is currently being investigated in various degenerative and inflammatory disorders such as Crohn's disease, graft-versus-host disease, diabetic nephropathy and organ fibrosis. The mechanisms by which MSCs exert their therapeutic effects are multifaceted, but in general, these cells are thought to enable damaged tissues to form a balanced inflammatory and regenerative microenvironment in the presence of vigorous inflammation...
June 12, 2018: Nature Reviews. Nephrology
Bingzong Li, Hongxia Xu, Huiying Han, Sha Song, Xiaojuan Zhang, Lu Ouyang, Chen'ao Qian, Yating Hong, Yingchun Qiu, Wenqi Zhou, Moli Huang, Wenzhuo Zhuang
Multiple myeloma (MM) is characterized by the decreased osteogenic potential of mesenchymal stem cells (MSCs). Communication between cancer cells and cancer stromal cells is a driving factor in tumor progression. Understanding the myeloma-stroma interactions is critical to the development of effective strategies that can reverse bone diseases. Here we identified that bioactive lncRNA RUNX2-AS1 in myeloma cells could be packed into exosomes and transmitted to MSCs, thus repressing the osteogenesis of MSCs. RUNX2-AS1, which arises from the antisense strand of RUNX2, was enriched in MSCs derived from MM patients (MM-MSCs)...
June 12, 2018: Oncogene
Dewan Zhao, Lingjia Liu, Qiang Chen, Fangfang Wang, Qiuyang Li, Qiang Zeng, Jingcao Huang, Maowen Luo, Wenxian Li, Yuhuan Zheng, Ting Liu
BACKGROUND: The physiological approach suggests that an environment associating mesenchymal stromal cells with low O2 concentration would be most favorable for the maintenance of hematopoietic stem/progenitor cells (HSPCs). To test this hypothesis, we performed a coculture of cord blood CD34+ cells with Wharton's jelly mesenchymal stem cells (WJ-MSCs) under different O2 concentration to simulate the growth of HSPCs in vivo, and assessed the impacts on stemness maintenance and proliferation of cord blood HSPCs in vitro...
June 13, 2018: Stem Cell Research & Therapy
Yen-An Tang, Yu-Feng Chen, Yi Bao, Sylvia Mahara, Siti Maryam J M Yatim, Gokce Oguz, Puay Leng Lee, Min Feng, Yu Cai, Ern Yu Tan, Sau Shung Fong, Zi-Huan Yang, Ping Lan, Xiao-Jian Wu, Qiang Yu
Colorectal cancer patients often relapse after chemotherapy, owing to the survival of stem or progenitor cells referred to as cancer stem cells (CSCs). Although tumor stromal factors are known to contribute to chemoresistance, it remains not fully understood how CSCs in the hypoxic tumor microenvironment escape the chemotherapy. Here, we report that hypoxia-inducible factor (HIF-1α) and cancer-associated fibroblasts (CAFs)-secreted TGF-β2 converge to activate the expression of hedgehog transcription factor GLI2 in CSCs, resulting in increased stemness/dedifferentiation and intrinsic resistance to chemotherapy...
June 11, 2018: Proceedings of the National Academy of Sciences of the United States of America
Patrick C Baer, Jürgen M Overath, Anja Urbschat, Ralf Schubert, Benjamin Koch, Asanke A Bohn, Helmut Geiger
Stem cell-based therapies require cells with a maximum regenerative capacity in order to support regeneration after tissue injury and organ failure. Optimization of this regenerative potential of mesenchymal stromal/stem cells (MSC) or their conditioned medium by in vitro preconditioning regimens are considered to be a promising strategy to improve the release of regenerative factors. In the present study, MSC were isolated from inguinal adipose tissue (mASC) from C57BL/6 mice, cultured, and characterized. Then, mASC were either preconditioned by incubation in a hypoxic environment (0...
June 10, 2018: International Journal of Molecular Sciences
Igor A Khlusov, Yuri Dekhtyar, Yurii P Sharkeev, Vladimir F Pichugin, Marina Y Khlusova, Nataliya Polyaka, Fedor Tyulkin, Viktorija Vendinya, Elena V Legostaeva, Larisa S Litvinova, Valeria V Shupletsova, Olga G Khaziakhmatova, Kristina A Yurova, Konstantin A Prosolov
Mesenchymal stem cells (MSCs) and osteoblasts respond to the surface electrical charge and topography of biomaterials. This work focuses on the connection between the roughness of calcium phosphate (CP) surfaces and their electrical potential (EP) at the micro- and nanoscales and the possible role of these parameters in jointly affecting human MSC osteogenic differentiation and maturation in vitro. A microarc CP coating was deposited on titanium substrates and characterized at the micro- and nanoscale. Human adult adipose-derived MSCs (hAMSCs) or prenatal stromal cells from the human lung (HLPSCs) were cultured on the CP surface to estimate MSC behavior...
June 9, 2018: Materials
Nadia Rbia, Liselotte F Bulstra, Allen T Bishop, Andre J van Wijnen, Alexander Y Shin
BACKGROUND: The addition of adipose-derived Mesenchymal Stromal Cells (MSCs) to decellularized nerve allografts may improve outcomes of nerve reconstruction. Prior techniques used for cell seeding are traumatic to both the MSCs and nerve graft. An adequate, reliable and validated cell seeding technique is an essential step for evaluating the translational utility of MSC-enhanced decellularized nerve grafts. The purpose of this study was to develop a simple seeding strategy with an optimal seeding duration...
May 22, 2018: Plastic and Reconstructive Surgery
Lei Sui, Ming Wang, Qianqian Han, Liming Yu, Lan Zhang, Leilei Zheng, Junxiang Lian, Jin Zhang, Paloma Valverde, Qiaobing Xu, Qisheng Tu, Jake Chen
Specific microRNAs (miRs) and the Wnt signaling pathway play critical roles in regulating bone development and homeostasis. Our previous studies revealed the ability of miR-335-5p to promote osteogenic differentiation by downregulating Wnt antagonist Dickkopf-1 (DKK1). The purpose of this study was to use nano-materials to efficiently deliver miR-335-5p into osteogenic cells for tissue engineering applications. We synthesized and screened a library of 12 candidate nano-lipidoids,of which L8 was identified as the preferred biodegradable lipidoid for miRNA molecule delivery into cells...
May 23, 2018: Biomaterials
Longzhen Piao, Zhaoting Yang, Jiajun Jin, Weidong Ni, Wenbo Qi, Yanhua Xuan
B7H4 is overexpressed in human cancers and often correlates with poor clinical outcome. There is a lack of data on the role of B7H4 as a cancer stem cell (CSC) regulator in esophageal squamous cell carcinoma (ESCC) and its expression levels compared to other stemness genes in ESCC. In this study, we have assessed the expression of B7H4 and cancer stemness proteins in 156 paraffin embedded ESCC tissue samples using immunohistochemistry as well as in ESCC cell lines using western blotting and immunofluorescence imaging...
June 6, 2018: Human Pathology
Mirasbek Kuterbekov, Paul Machillot, Pierre Lhuissier, Catherine Picart, Alain M Jonas, Karine Glinel
Porous polymeric microcarriers are a versatile class of biomaterial constructs with extensive use in drug delivery, cell culture and tissue engineering. Currently, most methods for their production require potentially toxic organic solvents with complex setups which limit their suitability for biomedical applications and their large-scale production. Herein, we report an organic, solvent-free method for the fabrication of porous poly(l-lactide) (PLLA) microcarriers. The method is based on the spherulitic crystallization of PLLA in its miscible blends with poly(ethylene glycol) (PEG)...
June 6, 2018: Acta Biomaterialia
Xiubo Fan, Dianyang Guo, Alice M S Cheung, Zhi Yong Poon, Chui Sun Yap, Shane Goh Ee, Dianyan Guo, Huihua Li, Sudipto Bari, Shang Li, Kiat Hon Lim, William Hwang Ying Khee
The immunosuppressive properties of mesenchymal stromal cells (MSCs) have been clinically proven to be effective in treating graft-versus-host disease (GVHD). However, MSC therapy is limited by the need for laborious and expensive manufacturing processes that are fraught with batch-to-batch variability. Substitution of MSC therapy with key MSC-mediated immunomodulatory factors could be an option for GVHD treatment. Using a simulated in vitro model of the immunosuppressive effects of MSC on allogeneic graft reactions, a synergistic two-factor combination (2FC) of CXCL5 and anti-CCL24 was identified from a panel of over 100 immunomodulatory factors as being superior to MSC in the modulation of mixed lymphocyte reactions...
June 5, 2018: Biology of Blood and Marrow Transplantation
Trivia Frazier, Stephen Lee, Annie Bowles, Julie Semon, Bruce Bunnell, Xiying Wu, Jeffrey Gimble
Adipose tissue is now recognized as a functional organ that contains cellular heterogeneity and diversity within anatomical depots. The stromal vascular fraction (SVF) of adipose contains endothelial progenitors, fibroblasts, lymphocytes, monocyte/macrophages, pericytes, pre-adipocytes, and stromal/stem cells, among others. In recent years, there has been a growing appreciation of the influence of age and gender in the field of stem cell biology. Yet few studies have evaluated the influence of biological age or sex on either SVF cell heterogeneity or immunophenotype...
June 8, 2018: Adipocyte
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