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stem cell stromal

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https://www.readbyqxmd.com/read/29451855/in-vivo-labeling-by-cd73-marks-multipotent-stromal-cells-and-highlights-endothelial-heterogeneity-in-the-bone-marrow-niche
#1
Martin Breitbach, Kenichi Kimura, Tiago C Luis, Christopher J Fuegemann, Petter S Woll, Michael Hesse, Raffaella Facchini, Sarah Rieck, Katarzyna Jobin, Julia Reinhardt, Osamu Ohneda, Daniela Wenzel, Caroline Geisen, Christian Kurts, Wolfgang Kastenmüller, Michael Hölzel, Sten E W Jacobsen, Bernd K Fleischmann
Despite much work studying ex vivo multipotent stromal cells (MSCs), the identity and characteristics of MSCs in vivo are not well defined. Here, we generated a CD73-EGFP reporter mouse to address these questions and found EGFP + MSCs in various organs. In vivo, EGFP + mesenchymal cells were observed in fetal and adult bones at proliferative ossification sites, while in solid organs EGFP + cells exhibited a perivascular distribution pattern. EGFP + cells from the bone compartment could be clonally expanded ex vivo from single cells and displayed trilineage differentiation potential...
February 1, 2018: Cell Stem Cell
https://www.readbyqxmd.com/read/29451220/critical-role-of-sdf-1-cxcr4-signaling-pathway-in-stem-cell-homing-in-the-deafened-rat-cochlea-after-acoustic-trauma
#2
Ali Asghar Peyvandi, Navid Ahmady Roozbahany, Hassan Peyvandi, Hojjat-Allah Abbaszadeh, Niloofar Majdinasab, Mohammad Faridan, Somayeh Niknazar
Previous animal studies have shown that stromal cell-derived factor-1 (SDF-1)/CXC chemokine receptor-4 (CXCR4) signaling pathway plays an important role in the targeted migration of bone marrow-derived mesenchymal stem cells (BMSCs) to the injured area. In the present study, we aimed to investigate the potential role of chemotactic SDF-1/CXCR4 signaling pathway in the homing of transplanted BMSCs to the injured cochlea after noise-induced hearing loss (NIHL) in a rat model. White noise exposure (110 dB) paradigm was used for hearing loss induction in male rats for 6 hours in 5 days...
January 2018: Neural Regeneration Research
https://www.readbyqxmd.com/read/29449679/tert-promoter-mutation-in-adult-granulosa-cell-tumor-of-the-ovary
#3
Jessica A Pilsworth, Dawn R Cochrane, Zhouchunyang Xia, Geraldine Aubert, Anniina E M Färkkilä, Hugo M Horlings, Satoshi Yanagida, Winnie Yang, Jamie L P Lim, Yi Kan Wang, Ali Bashashati, Jacqueline Keul, Adele Wong, Kevin Norris, Sara Y Brucker, Florin-Andrei Taran, Bernhard Krämer, Annette Staebler, Esther Oliva, Sohrab P Shah, Stefan Kommoss, Friedrich Kommoss, C Blake Gilks, Duncan M Baird, David G Huntsman
The telomerase reverse transcriptase (TERT) gene is highly expressed in stem cells and silenced upon differentiation. Cancer cells can attain immortality by activating TERT to maintain telomere length and telomerase activity, which is a crucial step of tumorigenesis. Two somatic mutations in the TERT promoter (C228T; C250T) have been identified as gain-of-function mutations that promote transcriptional activation of TERT in multiple cancers, such as melanoma and glioblastoma. A recent study investigating TERT promoter mutations in ovarian carcinomas found C228T and C250T mutations in 15...
February 15, 2018: Modern Pathology: An Official Journal of the United States and Canadian Academy of Pathology, Inc
https://www.readbyqxmd.com/read/29448000/bone-marrow-derived-fibrocytes-promote-stem-cell-like-properties-of-lung-cancer-cells
#4
Atsuro Saijo, Hisatsugu Goto, Mayuri Nakano, Atsushi Mitsuhashi, Yoshinori Aono, Masaki Hanibuchi, Hirohisa Ogawa, Hisanori Uehara, Kazuya Kondo, Yasuhiko Nishioka
Cancer stem cells (CSCs) represent a minor population that have clonal tumor initiation and self-renewal capacity and are responsible for tumor initiation, metastasis, and therapeutic resistance. CSCs reside in niches, which are composed of diverse types of stromal cells and extracellular matrix components. These stromal cells regulate CSC-like properties by providing secreted factors or by physical contact. Fibrocytes are differentiated from bone marrow-derived CD14 + monocytes and have features of both macrophages and fibroblasts...
February 12, 2018: Cancer Letters
https://www.readbyqxmd.com/read/29446455/uncultivated-stromal-vascular-fraction-is-equivalent-to-adipose-derived-stem-and-stromal-cells-on-porous-polyurethrane-scaffolds-forming-adipose-tissue-in-vivo
#5
Michael Griessl, Anna-Maria Buchberger, Sybille Regn, Kilian Kreutzer, Katharina Storck
OBJECTIVES/HYPOTHESIS: To find an alternative approach to contemporary techniques in tissue augmentation and reconstruction, tissue engineering strategies aim to involve adipose-derived stem and stromal cells (ASCs) harboring a strong differentiation potential into various tissue types such as bone, cartilage, and fat. STUDY DESIGN: Animal research. METHODS: The stromal vascular fraction (SVF) was used directly as a cell source to provide a potential alternative to contemporary ASC-based adipose tissue engineering...
February 15, 2018: Laryngoscope
https://www.readbyqxmd.com/read/29441735/the-role-of-mmu-mir-155-5p-nf-%C3%AE%C2%BAb-signaling-in-the-education-of-bone-marrow-derived-mesenchymal-stem-cells-by-gastric-cancer-cells
#6
Mei Wang, Fang Yang, Rong Qiu, Mengchu Zhu, Huiling Zhang, Wenrong Xu, Bo Shen, Wei Zhu
Bone marrow-derived mesenchymal stem cells (BM-MSCs) are important precursors of tumor stromal cells. Previously, we have demonstrated that miR-155-5p inhibition directly induced transition of BM-MSCs into gastric cancer-associated MSCs. Whether miR-155-5p is involved in the education of BM-MSCs by gastric cancer cells has not been established. Murine BM-MSCs (mMSCs) were isolated and grown in conditioned medium derived from gastric cancer cell line MFC (MFC-CM). The tumor-promoting phenotype and function of mMSCs were detected by immunofluorescence staining, quantitative reverse transcription-polymerase chain reaction (qRT-PCR), cell colony formation assay, transwell migration, and invasion assays...
February 14, 2018: Cancer Medicine
https://www.readbyqxmd.com/read/29440901/guided-osteoporotic-bone-regeneration-with-composite-scaffolds-of-mineralized-ecm-heparin-membrane-loaded-with-bmp2-related-peptide
#7
Tingfang Sun, Man Liu, Sheng Yao, Yanhui Ji, Lei Shi, Kai Tang, Zekang Xiong, Fan Yang, Kaifang Chen, Xiaodong Guo
Introduction: At present, the treatment of osteoporotic defects poses a great challenge to clinicians, owing to the lower regeneration capacity of the osteoporotic bone as compared with the normal bone. The guided bone regeneration (GBR) technology provides a promising strategy to cure osteoporotic defects using bioactive membranes. The decellularized matrix from the small intestinal submucosa (SIS) has gained popularity for its natural microenvironment, which induces cell response. Materials and methods: In this study, we developed heparinized mineralized SIS loaded with bone morphogenetic protein 2 (BMP2)-related peptide P28 (mSIS/P28) as a novel GBR membrane for guided osteoporotic bone regeneration...
2018: International Journal of Nanomedicine
https://www.readbyqxmd.com/read/29438465/does-stromal-vascular-fraction-ensure-a-higher-survival-in-autologous-fat-grafting-for-breast-augmentation-a-volumetric-study-using-3-dimensional-laser-scanning
#8
Cheng-Hung Chiu
Background: Cell-assisted lipotransfer (CAL) has been considered a promising technique for promoting adipogenesis and angiogenesis in fat grafts. Objectives: The author sought to objectively analyze the change of breast volume in patients who underwent stromal vascular fraction (SVF)-enriched fat grafting for breast augmentation and compared the clinical results with those who underwent conventional fat grafting without SVF by using 3D laser scanning. Methods: From April 2015 to March 2016, 105 patients who underwent traditional fat grafting without SVF enrichment for breast augmentation were assigned to group A and served as the control...
February 9, 2018: Aesthetic Surgery Journal
https://www.readbyqxmd.com/read/29438279/recent-updates-on-treatment-of-ocular-microbial-infections-by-stem-cell-therapy-a-review
#9
REVIEW
Seoh Wei Teh, Pooi Ling Mok, Munirah Abd Rashid, Mae-Lynn Catherine Bastion, Normala Ibrahim, Akon Higuchi, Kadarkarai Murugan, Rajan Mariappan, Suresh Kumar Subbiah
Ocular microbial infection has emerged as a major public health crisis during the past two decades. A variety of causative agents can cause ocular microbial infections; which are characterized by persistent and destructive inflammation of the ocular tissue; progressive visual disturbance; and may result in loss of visual function in patients if early and effective treatments are not received. The conventional therapeutic approaches to treat vision impairment and blindness resulting from microbial infections involve antimicrobial therapy to eliminate the offending pathogens or in severe cases; by surgical methods and retinal prosthesis replacing of the infected area...
February 13, 2018: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/29435977/characterization-of-mesenchymal-stem-stromal-cells-with-lymphoid-tissue-organizer-cell-potential-in-tonsils-from-children
#10
Alejandro Prados, Raquel Muñoz-Fernández, Pablo Fernandez-Rubio, Enrique G Olivares
Lymphoid tissue organizer (LTo) cells, identified in mouse and human embryos, are thought to be precursors of stromal cells in secondary lymphoid organs. Whether LTo cells are present in human adults, however remains unknown. We obtained 15 stromal cell lines from tonsils from children who underwent tonsillectomy, and studied the antigen phenotype of these tonsil stromal cell (TSC) lines by flow cytometry and RT-PCR. Cell lines met the minimal criteria proposed by the International Society for Cellular Therapy to define human mesenchymal stem/stromal cells (MSCs): plastic-adherent capacity; expression of CD73, CD90 and CD105, lack of CD45, CD19 and HLA-DR; and capacity to differentiate into adipocytes, osteoblasts and chondrocytes...
February 12, 2018: European Journal of Immunology
https://www.readbyqxmd.com/read/29433563/therapeutic-effect-of-human-umbilical-cord-derived-mesenchymal-stem-cells-on-injured-rat-endometrium-during-its-chronic-phase
#11
Lu Zhang, Ying Li, Chun-Yi Guan, Shi Tian, Xiao-Dan Lv, Jian-Hui Li, Xu Ma, Hong-Fei Xia
BACKGROUND: Repair deficiency after endometrial injury is an important reason for intra-uterine adhesions, amenorrhea, and infertility in females. Bone marrow-derived mesenchymal stem cell (BMSC) transplantation is effective in repairing the damaged endometrium. However, the possibility of using umbilical cord-derived MSCs (UC-MSCs) to treat endometrial injury is rarely reported. METHODS: Ethanol (95%) was injected into rat uterus to establish a model of endometrial injury...
February 13, 2018: Stem Cell Research & Therapy
https://www.readbyqxmd.com/read/29433375/the-cxcr4-antagonist-amd3100-promotes-mesenchymal-stem-cell-mobilization-in-rats-preconditioned-with-the-hypoxia-mimicking-agent-cobalt-chloride
#12
Lizhen Liu, Qin Yu, Shan Fu, Binsheng Wang, Kaimin Hu, Limengmeng Wang, Yongxian Hu, Yulin Xu, Xiaohong Yu, He Huang
Mobilization of mesenchymal stem cells (MSCs) is an attractive strategy for cell therapy. Our previous study demonstrated that MSCs can be mobilized in circulating blood by short-term hypoxia and hypoxia-inducible factor-1α (HIF-1α) is essential for MSC mobilization. In the present study, the effect of the hypoxia mimicking agent CoCl2 was examined on MSC mobilization. The results indicated that the frequency of circulating MSCs increased slightly by administration of CoCl2. However, the mobilization efficiency was low...
February 13, 2018: Stem Cells and Development
https://www.readbyqxmd.com/read/29431030/angiogenic-activity-of-human-adipose-derived-mesenchymal-stem-cells-under-simulated-microgravity
#13
Andrey Ratushnyy, Maria Ezdakova, Danila Yakubets, Ludmila Buravkova
Multipotent mesenchymal stem/stromal cells (MSCs) are strongly involved in tissue homeostasis mainly through paracrine regulation. Here we examined the influence of simulated microgravity on the angiogenic potential of adipose-derived MSCs (ASCs). The conditioned medium (CM) from RPM-exposed ASCs stimulated the formation of vessel network in ovo, endothelial cell (EC) capillary-like network ex ovo and non-directed EC migration in vitro. These effects were driven by alteration of both angiogenesis-related gene and protein expression...
February 12, 2018: Stem Cells and Development
https://www.readbyqxmd.com/read/29429944/engineering-stem-and-stromal-cell-therapies-for-musculoskeletal-tissue-repair
#14
REVIEW
Claudia Loebel, Jason A Burdick
Stem cells and tissue-derived stromal cells stimulate the repair of degenerated and injured tissues, motivating a growing number of cell-based interventions in the musculoskeletal field. Recent investigations have indicated that these cells are critical for their trophic and immunomodulatory role in controlling endogenous cells. This Review presents recent clinical advances where stem cells and stromal cells have been used to stimulate musculoskeletal tissue repair, including delivery strategies to improve cell viability and retention...
February 6, 2018: Cell Stem Cell
https://www.readbyqxmd.com/read/29429943/cd157-marks-tissue-resident-endothelial-stem-cells-with-homeostatic-and-regenerative-properties
#15
Taku Wakabayashi, Hisamichi Naito, Jun-Ichi Suehiro, Yang Lin, Hideya Kawaji, Tomohiro Iba, Tsukasa Kouno, Sachi Ishikawa-Kato, Masaaki Furuno, Kazuhiro Takara, Fumitaka Muramatsu, Jia Weizhen, Hiroyasu Kidoya, Katsuhiko Ishihara, Yoshihide Hayashizaki, Kohji Nishida, Mervin C Yoder, Nobuyuki Takakura
The generation of new blood vessels via angiogenesis is critical for meeting tissue oxygen demands. A role for adult stem cells in this process remains unclear. Here, we identified CD157 (bst1, bone marrow stromal antigen 1) as a marker of tissue-resident vascular endothelial stem cells (VESCs) in large arteries and veins of numerous mouse organs. Single CD157+ VESCs form colonies in vitro and generate donor-derived portal vein, sinusoids, and central vein endothelial cells upon transplantation in the liver...
February 1, 2018: Cell Stem Cell
https://www.readbyqxmd.com/read/29429941/quantitative-analysis-of-composite-umbilical-cord-tissue-health-using-a-standardized-explant-approach-and-an-assay-of-metabolic-activity
#16
Matthew L Skiles, Katherine S Brown, William Tatz, Kristen Swingle, Heather L Brown
BACKGROUND: Umbilical cord (UC) tissue can be collected in a noninvasive procedure and is enriched in progenitor cells with potential therapeutic value. Mesenchymal stromal cells (MSCs) can be reliably harvested from fresh or cryopreserved UC tissue by explant outgrowth with no apparent impact on functionality. A number of stem cell banks offer cryopreservation of UC tissue, alongside cord blood, for future cell-based applications. In this setting, measuring and monitoring UC quality is critical...
February 8, 2018: Cytotherapy
https://www.readbyqxmd.com/read/29428734/co-transplantation-of-exosomes-derived-from-hypoxia-preconditioned-adipose-mesenchymal-stem-cells-promotes-neovascularization-and-graft-survival-in-fat-grafting
#17
Yu-di Han, Yun Bai, Xin-Long Yan, Jing Ren, Quan Zeng, Xiao-Dong Li, Xue-Tao Pei, Yan Han
BACKGROUND: Adipose-derived stromal cells (ADSCs)-derived exosomes (ADSC-Exos) account for the proangiogenic potential of stem cell. This study aimed to investigate the effect of ADSC-derived exosomes (ADSC-Exos) on the survival in fat grafting. METHODS: A nude mouse model of subcutaneous fat grafting was adopted. Hypoxic preconditioned ADSC-Exos and ADSC-Exos were injected around the grafted tissue. The fat graft sample was weighed and examined by hematoxylin and eosin (H&E) staining and immunohistochemistry...
February 8, 2018: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/29428676/controlled-release-of-collagen-binding-sdf-1%C3%AE-from-the-collagen-scaffold-promoted-tendon-regeneration-in-a-rat-achilles-tendon-defect-model
#18
Jie Sun, Chenchen Mou, Qin Shi, Bing Chen, Xianglin Hou, Wen Zhang, Xiaoran Li, Yan Zhuang, Jiajia Shi, Yanyan Chen, Jianwu Dai
It had been demonstrated that stromal cell-derived factor-1α (SDF-1α) could promote in situ tendon regeneration by recruiting endogenous cells. However, native SDF-1α diffuses too fast in vivo, reducing its local concentration and efficacy. In this study, we prepared a recombinant SDF-1α containing a collagen-binding domain (CBD-SDF-1α) and developed a functional collagen scaffold by tethering CBD-SDF-1α on the collagen scaffold for in situ tendon regeneration. CBD-SDF-1α could induce the migration of mesenchymal stem cells, dermal fibroblasts and Achilles tendon fibroblasts in vitro, and achieve controlled release from the collagen scaffold...
February 4, 2018: Biomaterials
https://www.readbyqxmd.com/read/29427116/stem-cell-based-therapies-for-polyglutamine-diseases
#19
Liliana S Mendonça, Isabel Onofre, Catarina Oliveira Miranda, Rita Perfeito, Clévio Nóbrega, Luís Pereira de Almeida
Polyglutamine (polyQ) diseases are a family of neurodegenerative disorders with very heterogeneous clinical presentations, although with common features such as progressive neuronal death. Thus, at the time of diagnosis patients might present an extensive and irreversible neuronal death demanding cell replacement or support provided by cell-based therapies. For this purpose stem cells, which include diverse populations ranging from embryonic stem cells (ESCs), to fetal stem cells, mesenchymal stromal cells (MSCs) or induced pluripotent stem cells (iPSCs) have remarkable potential to promote extensive brain regeneration and recovery in neurodegenerative disorders...
2018: Advances in Experimental Medicine and Biology
https://www.readbyqxmd.com/read/29425184/bioreducible-polymer-micelles-based-on-acid-degradable-poly-ethylene-glycol-poly-amino-ketal-enhance-the-stromal-cell-derived-factor-1%C3%AE-gene-transfection-efficacy-and-therapeutic-angiogenesis-of-human-adipose-derived-stem-cells
#20
Tae-Jin Lee, Min Suk Shim, Taekyung Yu, Kyunghee Choi, Dong-Ik Kim, Soo-Hong Lee, Suk Ho Bhang
Adipose-derived stem cells (ADSCs) have the potential to treat ischemic diseases. In general, ADSCs facilitate angiogenesis by secreting various pro-angiogenic growth factors. However, transplanted ADSCs have a low therapeutic efficacy in ischemic tissues due to their poor engraftment and low viability. Stromal cell-derived factor-1α (SDF-1α) improves the survival rate of stem cells transplanted into ischemic regions. In this study, we developed acid-degradable poly(ethylene glycol)-poly(amino ketal) (PEG-PAK)-based micelles for efficient intracellular delivery of SDF-1α plasmid DNA...
February 9, 2018: International Journal of Molecular Sciences
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