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Colletotrichum

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https://www.readbyqxmd.com/read/27921398/effects-of-rhizobacteria-paenibacillus-polymyxa-apec136-and-bacillus-subtilis-apec170-on-biocontrol-of-postharvest-pathogens-of-apple-fruits
#1
Young Soo Kim, Kotnala Balaraju, Yongho Jeon
In this study, plant growth-promoting rhizobacteria (PGPR) were evaluated as potential biocontrol agents against postharvest pathogens of apple fruits. In vitro bioassays revealed that, out of 30 isolates screened, isolates APEC136 and APEC170 had the most significant inhibitory effects against the mycelial growth of several fungal pathogens. Analysis of 16S ribosomal RNA (rRNA) sequences identified the two effective isolates as Paenibacillus polymyxa and Bacillus subtilis, respectively. The two strains showed greater growth in brain-heart infusion broth than in other growth media...
2016: Journal of Zhejiang University. Science. B
https://www.readbyqxmd.com/read/27917337/identification-characterization-and-phylogenetic-analysis-of-antifungal-trichoderma-from-tomato-rhizosphere
#2
Shalini Rai, Prem Lal Kashyap, Sudheer Kumar, Alok Kumar Srivastava, Pramod W Ramteke
The use of Trichoderma isolates with efficient antagonistic activity represents a potentially effective and alternative disease management strategy to replace health hazardous chemical control. In this context, twenty isolates were obtained from tomato rhizosphere and evaluated by their antagonistic activity against four fungal pathogens (Fusarium oxysporum f. sp. lycopersici, Alternaria alternata, Colletotrichum gloeosporoides and Rhizoctonia solani). The production of extracellular cell wall degrading enzymes of tested isolates was also measured...
2016: SpringerPlus
https://www.readbyqxmd.com/read/27892688/secondary-metabolites-from-colletotrichum-capsici-an-endophytic-fungus-derived-from-siegesbeckia-pubescens-makino
#3
Fuqian Wang, Haoran Ma, Zhengxi Hu, Jie Jiang, Hucheng Zhu, Lu Cheng, Quanwei Yang, Hong Zhang, Geng Zhang, Yonghui Zhang
A rare new tremulane sesquiterpenoid analogue, 11,12-epoxy-5,6-seco-1,6(13)-tremuladien-5,12-olide (1), together with five known altenuene derivatives (2-6) was isolated from the cultures of Colletotrichum capsici, which was isolated as an endophytic fungus from fresh leaves of Siegesbeckia pubescens Makino (Compositae). Their structures were elucidated by means of spectroscopic methods and comparison with literature data. All compounds isolated were reported for the first time from the fungus C. capsici.
November 28, 2016: Natural Product Research
https://www.readbyqxmd.com/read/27867266/a-new-antifungal-isocoumarin-from-the-endophytic-fungus-trichoderma-sp-09-of-myoporum-bontioides-a-gray
#4
Wensheng Li, Jiaxin Xu, Fenqi Li, Li Xu, Chunyuan Li
BACKGROUND: Myoporum bontioides A. Gray is a commonly used medicinal plant in China. Recently, the chemical and bioactive investigations to the endophytic fungi of this plant have led to several new compounds with antimicrobial and cytotoxic activities. To find out more active molecules, the metabolites of an endophytic fungus, Trichoderma sp. 09 from the root of Myoporum bontioides were investigated. MATERIALS AND METHODS: The metabolites were isolated by column chromatography on silica gel, and their structures were elucidated on the basis of spectroscopic analysis[one-dimensional (1D), two-dimensional (2D)-nuclear magnetic resonance (NMR), Mass spectrometry (MS)], and by comparison with the published data...
October 2016: Pharmacognosy Magazine
https://www.readbyqxmd.com/read/27863465/bacillus-species-bt42-isolated-from-coffea-arabica-l-rhizosphere-antagonizes-colletotrichum-gloeosporioides-and-fusarium-oxysporum-and-also-exhibits-multiple-plant-growth-promoting-activity
#5
Tekalign Kejela, Vasudev R Thakkar, Parth Thakor
BACKGROUND: Colletotrichum and Fusarium species are among pathogenic fungi widely affecting Coffea arabica L., resulting in major yield loss. In the present study, we aimed to isolate bacteria from root rhizosphere of the same plant that is capable of antagonizing Colletotrichum gloeosporioides and Fusarium oxysporum as well as promotes plant growth. RESULTS: A total of 42 Bacillus species were isolated, one of the isolates named BT42 showed maximum radial mycelial growth inhibition against Colletotrichum gloeosporioides (78%) and Fusarium oxysporum (86%)...
November 18, 2016: BMC Microbiology
https://www.readbyqxmd.com/read/27859958/phylogeny-and-variability-of-colletotrichum-truncatum-associated-soybean-anthracnose-in-brazil
#6
Flávia Rogério, Maisa Ciampi-Guillardi, Marina Coan Goldoni Barbieri, Carlos Augusto Dórea Bragança, Claudine Dinali Santos Seixas, Álvaro Manuel Rodrigues Almeida, Nelson Sidnei Massola
AIMS: Fungal diseases are among the main factors limiting high yields of soybean crop. Colletotrichum isolates from soybean plants with anthracnose symptoms were studied from different regions and time periods in Brazil using molecular, morphological and pathogenic analyses. METHODS AND RESULTS: Bayesian phylogenetic inference of GAPDH, HIS3 and ITS-5.8S rDNA sequences, the morphologies of colony and conidia and inoculation tests on seeds and seedlings were performed...
November 8, 2016: Journal of Applied Microbiology
https://www.readbyqxmd.com/read/27856319/elucidation-of-biocontrol-mechanisms-of-trichoderma-harzianum-against-different-plant-fungal-pathogens-universal-yet-host-specific-response
#7
Vivek Sharma, Richa Salwan, Prem N Sharma, S S Kanwar
In the present study, different transcripts of Trichoderma harzianum ThHP-3 were evaluated for their response against four fungal pathogens Fusarium oxysporum, Colletotrichum capsici, Colletotrichum truncatum and Gloesercospora sorghi using RT-qPCR. The time course study of T. harzianum transcripts related to signal transduction, lytic enzymes, secondary metabolites and various transporters revealed variation in expression against four fungal pathogens. In a broader term, the transcripts were upregulated at various time intervals but the optimum expression of cyp3, abc, nrp, tga1, pmk, ech42 and glh20 varied with respect to host fungi...
November 14, 2016: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/27829044/transcriptome-profiling-of-the-phaseolus-vulgaris-colletotrichum-lindemuthianum-pathosystem
#8
Bilal A Padder, Kelvin Kamfwa, Halima E Awale, James D Kelly
Bean (Phaseolus vulgaris) anthracnose caused by the hemi-biotrophic pathogen Colletotrichum lindemuthianum is a major factor limiting production worldwide. Although sources of resistance have been identified and characterized, the early molecular events in the host-pathogen interface have not been investigated. In the current study, we conducted a comprehensive transcriptome analysis using Illumina sequencing of two near isogenic lines (NILs) differing for the presence of the Co-1 gene on chromosome Pv01 during a time course following infection with race 73 of C...
2016: PloS One
https://www.readbyqxmd.com/read/27819539/direct-estimation-of-local-ph-change-at-infection-sites-of-fungi-in-potato-tubers
#9
R Tardi-Ovadia, R Linker, L Tsror Lahkim
Fungi can modify the pH in or around the infected site via alkalization or acidification, and pH monitoring may provide valuable information on host-fungus interactions. The objective of the present study was to examine the ability of two fungi, Colletotrichum coccodes and Helminthosporium solani, to modify the pH of potato tubers during artificial inoculation in situ. Both fungi cause blemishes on potato tubers, which downgrades tuber quality and yield. Direct visualization and estimation of pH changes near the inoculation area were achieved using pH indicators and image analysis...
November 7, 2016: Phytopathology
https://www.readbyqxmd.com/read/27813588/purification-biochemical-characterization-and-antimicrobial-activity-of-a-new-lipid-transfer-protein-from-coffea-canephora-seeds
#10
G C V Bard, U Zottich, T A M Souza, S F F Ribeiro, G B Dias, S Pireda, M Da Cunha, R Rodrigues, L S Pereira, O L T Machado, A O Carvalho, V M Gomes
Coffee, an agronomical crop of great economic importance, is also among the most commonly traded commodities in worldwide markets. Antimicrobial peptides, which play a role in plant defense, have been identified and isolated particularly from seeds. We isolated and immunolocalized Cc-LTP2, a new lipid transfer protein (LTP) from Coffea canephora seeds. We report its antimicrobial activity against various phytopathogenic fungi of economic importance, and against the bacterium Xanthomonas euvesicatoria. Peptides from C...
October 24, 2016: Genetics and Molecular Research: GMR
https://www.readbyqxmd.com/read/27804104/bacillus-subtilis-based-formulation-for-the-control-of-postbloom-fruit-drop-of-citrus
#11
Mariana Nadjara Klein, Aline Caroline da Silva, Katia Cristina Kupper
Postbloom fruit drop (PFD) caused by Colletotrichum acutatum affects flowers and causes early fruit drop in all commercial varieties of citrus. Biological control with the isolate ACB-69 of Bacillus subtilis has been considered as a potential method for controlling this disease. This study aimed to develop and optimize a B. subtilis based-formulation with a potential for large-scale applications and evaluate its effect on C. acutatum in vitro and in vivo. Bacillus subtilis based-formulations were developed using different carrier materials, and their ability to control PFD was evaluated...
December 2016: World Journal of Microbiology & Biotechnology
https://www.readbyqxmd.com/read/27795709/biological-characteristics-and-antimicrobial-activity-of-endophytic-streptomyces-sp-tqr12-4-isolated-from-elite-citrus-nobilis-cultivar-ham-yen-of-vietnam
#12
Phan Thi Hong-Thao, Nguyen Vu Mai-Linh, Nguyen Thi Hong-Lien, Nguyen Van Hieu
Ham Yen orange (Citrus nobilis Lour) is the highly valuable commercial fruit of Vietnam. With the blooming of fruit production and farming area, this specialty crop is facing threats from several serious diseases; therefore the search for new effective biocontrollers is required to prevent the existing excessive use of fertilizers and plant protection chemicals. Endophytic actinomycetes are of great scientific interest due to their high potential of application in agriculture and pharmaceutical research. In this work, endophytic actinomycetes were isolated from a native orange species of Northeast mountainous province Tuyen Quang...
2016: International Journal of Microbiology
https://www.readbyqxmd.com/read/27792456/antimicrobial-activity-and-chemical-composition-of-three-essential-oils-extracted-from-mediterranean-aromatic-plants
#13
Hazem S Elshafie, Shimaa Sakr, Stefania M Mang, Sandra Belviso, Vincenzo De Feo, Ippolito Camele
There is a growing interest in essential oils (EOs) as possible alternatives for traditional chemical pesticides. This study was carried out to characterize the chemical composition of the three EOs extracted from Verbena officinalis, Majorana hortensis, and Salvia officinalis using gas chromatography (GC) and GC-mass spectrometry (GC-MS) and to evaluate in vitro their efficacy against some phyto or human pathogens. The antifungal activity was investigated against Colletotrichum acutatum and Botrytis cinerea in comparison with Azoxystrobin as a large spectrum fungicide...
October 28, 2016: Journal of Medicinal Food
https://www.readbyqxmd.com/read/27782129/diverse-colletotrichum-species-cause-anthracnose-of-tea-plants-camellia-sinensis-l-o-kuntze-in-china
#14
Yu-Chun Wang, Xin-Yuan Hao, Lu Wang, Bin Xiao, Xin-Chao Wang, Ya-Jun Yang
Anthracnose caused by Colletotrichum is one of the most severe diseases that can afflict Camellia sinensis. However, research on the diversity and geographical distribution of Colletotrichum in China remain limited. In this study, 106 Colletotrichum isolates were collected from diseased leaves of Ca. sinensis cultivated in the 15 main tea production provinces in China. Multi-locus phylogenetic analysis coupled with morphological identification showed that the collected isolates belonged to 11 species, including 6 known species (C...
October 26, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27746765/chilli-anthracnose-the-epidemiology-and-management
#15
Amrita Saxena, Richa Raghuwanshi, Vijai Kumar Gupta, Harikesh B Singh
Indian cuisine is renowned and celebrated throughout the world for its spicy treat to the tongue. The flavor and aroma of the food generated due to the use of spices creates an indelible experience. Among the commonly utilized spices to stimulate the taste buds in Indian food, whole or powdered chilli constitutes an inevitable position. Besides being a vital ingredient of of Indian food, chilli occupy an important position as an economic commodity, a major share in Indian economy. Chilli also has uncountable benefits to human health...
2016: Frontiers in Microbiology
https://www.readbyqxmd.com/read/27745991/synthesis-of-novel-tetrazole-derivatives-and-evaluation-of-their-antifungal-activity
#16
Edyta Łukowska-Chojnacka, Jolanta Mierzejewska, Małgorzata Milner-Krawczyk, Małgorzata Bondaryk, Monika Staniszewska
With the appearance of the antifungal resistance, novel antifungal agents need to be identified. In this context new 2,5-disubstituted tetrazole derivatives containing benzothiazole, benzoxazole or phenylsulfonyl moiety were synthesized by N-alkylation of aryltetrazole with 2-[(3-chloropropyl)sulfanyl]-1,3-benzothiazole or 2-[(3-chloropropyl)sulfanyl]-1,3-benzoxazole and Michael-type addition of aryltetrazole to phenyl vinyl sulfone. The chemical structures of the synthesized compounds were confirmed by means of (1)H NMR, (13)C NMR, IR and HRMS spectral data...
November 15, 2016: Bioorganic & Medicinal Chemistry
https://www.readbyqxmd.com/read/27728853/antifungal-activity-of-1-methylcyclopropene-1-mcp-against-anthracnose-colletotrichum-gloeosporioides-in-postharvest-mango-fruit-and-its-possible-mechanisms-of-action
#17
Xiangbin Xu, Huanhuan Lei, Xiuyan Ma, Tongfei Lai, Hongmiao Song, Xuequn Shi, Jiangkuo Li
Anthracnose caused by Colletotrichum gloeosporioides is one of the most important postharvest diseases in mango fruit, often causing huge economic losses. In this study, the effect of 1-methylcyclopropene (1-MCP) against anthracnose in postharvest mango fruit and the mechanisms involved were investigated. 1-MCP induced reactive oxygen species (ROS) generation, damaged the mitochondria and destroyed the integrity of plasma membrane of spores of C. gloeosporioides, significantly suppressing spore germination and mycelial growth of C...
October 6, 2016: International Journal of Food Microbiology
https://www.readbyqxmd.com/read/27721696/postharvest-disease-control-of-colletotrichum-gloeosporioides-and-penicillium-expansum-on-stored-apples-by-gamma-irradiation-combined-with-fumigation
#18
Wonsu Cheon, Young Soo Kim, Kotnala Balaraju, Bong-Su Kim, Byeong-Ho Lee, Yongho Jeon
To study the control of postharvest decay caused by Colletotrichum gloeosporioides and Penicillium expansum, gamma irradiation alone or in combination with fumigation was evaluated to extend the shelf life of apples in South Korea. An irradiation dose of 2.0 kGy resulted in the maximum inhibition of C. gloeosporioides and P. expansum spore germination. The gamma irradiation dose required to reduce the spore germination by 90% was 0.22 and 0.35 kGy for C. gloeosporioides and P. expansum, respectively. Microscopic observations revealed that when the fungal spores were treated with gamma irradiation (4...
October 2016: Plant Pathology Journal
https://www.readbyqxmd.com/read/27706702/mixed-inheritance-in-the-genetic-control-of-ramulosis-colletotrichum-gossypii-var-cephalosporioides-resistance-in-cotton
#19
M A C Oliveira, J B Duarte, C L Morello, N D Suassuna, A B Oliveira
Ramulosis is one of the most aggressive diseases in cotton, and understanding the genetic control of its resistance is imperative for selecting superior cotton genotypes in breeding programs. This study analyzed the inheritance pattern of this resistance using chi-square goodness-of-fit tests to determine the phenotypic proportions of the F2 generation, and a mixed inheritance approach to jointly model major gene and polygenes effects. F1, F2, Rc1, and Rc2 generations were obtained by crossing resistant (BRS Facual, CNPA 2984, or CNPA 2043) and susceptible (Delta Opal, CNPA 999, or CNPA 2161) genotypes, and were assessed under field conditions with artificial inoculation of the pathogen (Colletotrichum gossypii var...
September 2, 2016: Genetics and Molecular Research: GMR
https://www.readbyqxmd.com/read/27693762/the-characterization-of-1-4-bromophenyl-5-phenyl-1h-1-2-3-triazole-on-acute-toxicity-antimicrobial-activities-photophysical-property-and-binding-to-two-globular-proteins
#20
He Liu, Qiang Lin, Yixian Xie, Huoming Shu, Bo Li, Ge Gao, Kai Xiao, Xiaojun Yao, Runcong Dong, Yanlin Liu, Mengxiong He, Luyong Wu, Zhenfan Sun, Wenying He
1-(4-Bromophenyl)-5-phenyl-1H-1,2,3-triazole (BPT) was a newly synthesized compound. The acute toxicities of BPT to mice by intragastric administration have been determined and the result indicates that the intragastric administration of BPT did not produce any significant toxic effect on Kunming strain mice. It is also evaluated for the antimicrobial activity of BPT against three kinds of plant mycoplasma, Fusarium Wilt (race 4), Colletotrichum gloeosporioides Penz. and Xanthomonas oryzae by different method in vitro...
September 24, 2016: Journal of Photochemistry and Photobiology. B, Biology
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