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Meiyu Wang, Zongshan Zhou, Jianyuan Wu, Zhirui Ji, Junxiang Zhang
Fruit rot caused mainly by Colletotrichum gloeosporioides is a major cause of pre- and/or post-harvest diseases, which seriously constrains production, marketing, and export of fruits. To infect the host, this fungus evolves a specialized infection structure called the appressorium. Extensive past studies have characterized many appressorium-related genes in C. gloeosporioides, separately. However, a comprehensive understanding of the genes contributing to appressorium formation is far from complete. Here, global changes in gene expression were analyzed between appressoria and hyphae using RNA-Seq...
May 21, 2018: Gene
Shuning Chen, Yunyun Wang, Guido Schnabel, Congyue Annie Peng, Satyanarayana Lagishetty, Kerry Smith, Chao-Xi Luo, Huizhu Yuan
Anthracnose disease, caused by Colletotrichum truncatum, affects marketable yield during preharvest production and postharvest storage of fruits and vegetables worldwide. Demethylation inhibitor fungicides (DMIs) are among the very few chemical classes of single-site mode of action fungicides that are effective in controlling anthracnose disease. However, some species are inherently resistant to DMIs and more information is needed to understand this phenomenon. Isolates of C. truncatum were collected from the USA and China from peach, soybean, citrus, and begonia and sensitivity to six DMIs (difenoconazole, propiconazole, metconazole, tebuconazole, flutriafol and fenbuconazole) was determined...
May 24, 2018: Phytopathology
Ji Hoon Song, Changyeol Lee, Dahae Lee, Soonok Kim, Sunghee Bang, Myoung-Sook Shin, Jun Lee, Ki Sung Kang, Sang Hee Shim
Colletotrichum sp. JS-0367 was isolated from Morus alba (mulberry), identified, and cultured on a large scale for chemical investigation. One new anthraquinone (1) and three known anthraquinones (2-4) were isolated and identified using spectroscopic methods including 1D/2D-NMR and HRESIMS. Although the neuroprotective effects of some anthraquinones have been reported, the biological activities of the four anthraquinones isolated in this study have not been reported. Therefore, the neuroprotective effects of these compounds were determined against murine hippocampal HT22 cell death induced by glutamate...
May 23, 2018: Journal of Natural Products
Guillaume P Robin, Jochen Kleemann, Ulla Neumann, Lisa Cabre, Jean-Félix Dallery, Nicolas Lapalu, Richard J O'Connell
The genome of the hemibiotrophic anthracnose fungus, Colletotrichum higginsianum , encodes a large inventory of putative secreted effector proteins that are sequentially expressed at different stages of plant infection, namely appressorium-mediated penetration, biotrophy and necrotrophy. However, the destinations to which these proteins are addressed inside plant cells are unknown. In the present study, we selected 61 putative effector genes that are highly induced in appressoria and/or biotrophic hyphae. We then used Agrobacterium -mediated transformation to transiently express them as N -terminal fusions with fluorescent proteins in cells of Nicotiana benthamiana for imaging by confocal microscopy...
2018: Frontiers in Plant Science
R I Ventura-Aguilar, S Bautista-Baños, G Flores-García, L Zavaleta-Avejar
The antifungal effect of chitosan edible coatings (ChEC) functionalized with cinnamon essential oil and aqueous extract of Roselle calyces on Colletotrichum fragariae growth and physical-chemical, physiological and nutraceutical features of strawberries at 5 and 20 °C were evaluated. ChEC was characterized with respect to its water vapor permeability (WVP) and mechanical properties. Results indicated that C. fragariae grew from the third day in strawberries stored at 20 °C, whilst at 5 °C disease symptoms were observed after 10 days in fruit inoculated and treated with ChEC after 24 h...
October 1, 2018: Food Chemistry
Carolina Coronado-Ruiz, Roberto Avendaño, Efraín Escudero-Leyva, Geraldine Conejo-Barboza, Priscila Chaverri, Max Chavarría
The archive of the Universidad de Costa Rica maintains a nineteenth-century French collection of drawings and lithographs in which the biodeterioration by fungi is rampant. Because of nutritional conditions in which these fungi grew, we suspected that they possessed an ability to degrade cellulose. In this work our goal was to isolate and identify the fungal species responsible for the biodegradation of a nineteenth-century art collection and determine their cellulolytic activity. Fungi were isolated using potato-dextrose-agar (PDA) and water-agar with carboxymethyl cellulose (CMC)...
May 10, 2018: Scientific Reports
Zhihui Wu, Zihui Xie, Manlin Wu, Xiaoqi Li, Weilin Li, Weijia Ding, Zhigang She, Chunyuan Li
Six new cyclopentenone derivatives (+)-nigrosporione A, (+)-1, (‒)-nigrosporione A, (‒)-1, nigrosporione B (2), nigrosporione C (3), (+)-nigrosporione D, (+)-4, and (‒)-nigrosporione D, (‒)-4 were isolated from an endophytic fungus Nigrospora sphaerica ZMT05, collected from the rice grasshopper (Oxya chinensis Thunberg) which is an insect pest in rice and also used as a food for people in some countries. Their planar and spatial structures were determined by spectroscopic analyses and ECD calculations...
May 10, 2018: Journal of Agricultural and Food Chemistry
N M R Ashwin, Leonard Barnabas, Amalraj Ramesh Sundar, Palaniyandi Malathi, Rasappa Viswanathan, Antonio Masi, Ganesh Kumar Agrawal, Randeep Rakwal
Colletotrichum falcatum, a hemibiotrophic fungal pathogen, causes one of the major devastating diseases of sugarcane-red rot. C. falcatum secretes a plethora of molecular signatures that might play a crucial role during its interaction with sugarcane. Here, we report the purification and characterization of a novel secreted protein of C. falcatum that elicits defense responses in sugarcane and triggers hypersensitive response (HR) in tobacco. The novel protein purified from the culture filtrate of C. falcatum was identified by MALDI TOF/TOF MS and designated as C...
May 4, 2018: Applied Microbiology and Biotechnology
Lifeng Zhai, Meixin Zhang, Ni Hong, Feng Xiao, Min Fu, Jun Xiang, Guoping Wang
A novel hepta-segmented double-stranded RNA (dsRNA) virus was isolated and characterized from the strain FJ-4 of the phytopathogenic fungus Colletotrichum fructicola , and was named Colletotrichum fructicola chrysovirus 1 (CfCV1). The full-length cDNAs of dsRNA1-7 were 3620, 2801, 2687, 2437, 1750, 1536, and 1211 bp, respectively. The 5'- and 3'-untranslated regions of the seven dsRNAs share highly similar internal sequence and contain conserved sequence stretches, indicating that they have a common virus origin...
2018: Frontiers in Microbiology
Marina Arredondo-Santoyo, Ma Soledad Vázquez-Garcidueñas, Gerardo Vázquez-Marrufo
The isolation and characterization of fungal strains from poorly described taxa allows undercover attributes of their basic biology useful for biotechnology. Here, a wild fungal strain (CMU-196) from recently described Paraconiothyrium genus was analyzed. CMU-196 was identified as Paraconiothyrium brasiliense by phylogenetic analysis of the rDNA internal transcribed spacer region (ITS). CMU-196 metabolized 57 out of 95 substrates of the Biolog FF microplates. Efficient assimilation of dextrins and glycogen indicates that CMU-196 is a good producer of amylolytic enzymes...
April 30, 2018: Biotechnology Progress
Xiaolian Wang, Xin Xu, Yingmei Liang, Yonglin Wang, Chengming Tian
The Rho GTPase Cdc42 is conserved in fungi and plays a key role in regulating polarity establishment, morphogenesis and differentiation. In this study, we identified an ortholog of Cdc42, CgCdc42, and functionally characterized it to determine the role of Cdc42 in the development and pathogenicity of Colletotrichum gloeosporioides, a causal agent of poplar anthracnose. Targeted deletion of CgCdc42 resulted in reduced vegetative growth and dramatic morphological defects, including the formation of elongated conidia and abnormally shaped appressoria...
April 26, 2018: Current Genetics
Xiaofei Liang, Bo Wang, Qiuyue Dong, Lingnan Li, Jeffrey A Rollins, Rong Zhang, Guangyu Sun
The fungal genus Colletotrichum contains hemibiotrophic phytopathogens being highly variable in host and tissue specificities. We sequenced a C. fructicola genome (1104-7) derived from an isolate of apple in China and compared it with the reference genome (Nara_gc5) derived from an isolate of strawberry in Japan. Mauve alignment and BlastN search identified 0.62 Mb lineage-specific (LS) genomic regions in 1104-7 with a length criterion of 10 kb. Genes located within LS regions evolved more dynamically, and a strongly elevated proportion of genes were closely related to non-Colletotrichum sequences...
2018: PloS One
S K Kimaru, E Monda, R C Cheruiyot, J Mbaka, A Alakonya
Anthracnose disease of avocado contributes to a huge loss of avocado fruits due to postharvest rot in Kenya. The causal agent of this disease has not been clear but presumed to be Colletotrichum gloeosporioides as reported in other regions where avocado is grown. The fungus mainly infects fruits causing symptoms such as small blackish spots, "pepper spots," and black spots with raised margin which coalesce as infection progresses. Due to economic losses associated with the disease and emerging information of other species of fungi as causal agents of the disease, this study was aimed at identifying causal agent(s) of the disease...
2018: International Journal of Microbiology
Junsong Pan, Junyi Tan, Yuhui Wang, Xiangyang Zheng, Ken Owens, Dawei Li, Yuhong Li, Yiqun Weng
Map-based cloning identified a candidate gene for resistance to the anthracnose fungal pathogen Colletotrichum orbiculare in cucumber, which reveals a novel function for the highly conserved STAYGREEN family genes for host disease resistance in plants. Colletotrichum orbiculare is a hemibiotrophic fungal pathogen that causes anthracnose disease in cucumber and other cucurbit crops. No host resistance genes against the anthracnose pathogens have been cloned in crop plants. Here, we reported fine mapping and cloning of a resistance gene to the race 1 anthracnose pathogen in cucumber inbred lines Gy14 and WI 2757...
April 21, 2018: TAG. Theoretical and Applied Genetics. Theoretische und Angewandte Genetik
Riccardo Baroncelli, Serenella Sukno, Sabrina Sarrocco, Giovanni Cafà, Gaetan Le Floch, Michael R Thon
Colletotrichum orchidophilum is a plant pathogenic fungus infecting a wide range of plant species belonging to the family Orchidaceae. Besides its economic impact, C. orchidophilum has been used in recent years in evolutionary studies as it represents the closest related species to the C. acutatum species complex. Here we present the first draft whole-genome sequence of C. orchidophilum IMI 309357, providing a resource for future research on anthracnose of Orchidaceae and other hosts.
April 12, 2018: Molecular Plant-microbe Interactions: MPMI
Su Jeung Kim, Eun Ju Ko, Jeum Kyu Hong, Yong Chull Jeun
Chlorella , one single-cell green algae organism that lives autotrophically by photosynthesis, can directly suppress some plant diseases. The objective of this study was to determine whether pre-spraying with Chlorella fusca suspension could induce systemic acquired resistance (SAR) in cucumber plants against anthracnose caused by Colletotrichum orbiculare . In order to illustrate SAR induced by algae, infection structures in host cells were observed under a transmission electron microscope (TEM). Cytological changes as defense responses of host mesophyll cells such as accumulation of vesicles, formation of sheath around penetration hyphae, and thickness of cell wells adjoining with intracellular hyphae were demonstrated in cucumber leaves...
April 2018: Plant Pathology Journal
Hafiz Husnain Nawaz, M J Nelly Rajaofera, Qiguang He, Usmani Anam, Chunhua Lin, Weiguo Miao
Anthracnose disease in the cotton plant caused by fungal pathogen Colletotrichum gossypii. It is supposed to be most critical diseases in the cotton crop as it causes infection and leads to complete damaging of the cotton crop by infecting the leaves, stems, and bolls in the field. The disease control is challenging due to the absence of an effective fungicide without damaging the farmer health and environment. So the series of experiments were designed to assess the antagonistic activity of biosurfactant released by strain Bacillus licheniformis OE-04 against the anthracnose causing agent in cotton and this strain was screened out from forty eight strain of rhizobacteria...
April 2018: Pesticide Biochemistry and Physiology
Lizeth Guardado-Valdivia, Erik Tovar-Pérez, Alejandra Chacón-López, Ulises López-García, Porfirio Gutiérrez-Martínez, Alexandra Stoll, Selene Aguilera
Anthracnose is a fungal disease caused by Colletotrichum species that is detrimental to numerous fruit, including soursop and avocado. The use of fungicides to maintain the high quality of fruit creates a potential health risk. One alternative to this problem is the biological control, which has been applied successfully during postharvest. The Bacillus species are one of the most studied biological agents against postharvest pathogens because accomplish their biocontrol performance by producing a variety of metabolites...
May 2018: Microbiological Research
Yuchun Wang, Xinyuan Hao, Qinhua Lu, Lu Wang, Wenjun Qian, Nana Li, Changqing Ding, Xinchao Wang, Yajun Yang
Anthracnose causes severe losses of tea production in China. Although genes and biological processes involved in anthracnose resistance have been reported in other plants, the molecular response to anthracnose in tea plant is unknown. We used the susceptible tea cultivar Longjing 43 and the resistant cultivar Zhongcha 108 as materials and compared transcriptome changes in the leaves of both cultivars following Colletotrichum fructicola inoculation. In all, 9015 and 8624 genes were differentially expressed between the resistant and susceptible cultivars and their controls (0 h), respectively...
2018: Horticulture Research
Lu Wang, Lina Yao, Xinyuan Hao, Nana Li, Wenjun Qian, Chuan Yue, Changqing Ding, Jianming Zeng, Yajun Yang, Xinchao Wang
Thirteen SWEET transporters were identified in Camellia sinensis and the cold-suppression gene CsSWEET16 contributed to sugar compartmentation across the vacuole and function in modifying cold tolerance in Arabidopsis. The sugars will eventually be exported transporters (SWEET) family of sugar transporters in plants is a recently identified protein family of sugar uniporters that contain seven transmembrane helices harbouring two MtN3 motifs. SWEETs play important roles in various biological processes, including plant responses to environmental stimuli...
April 3, 2018: Plant Molecular Biology
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