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Hemopexin

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https://www.readbyqxmd.com/read/29081299/isolation-identification-and-characterization-of-a-new-type-of-lectin-with-%C3%AE-amylase-inhibitory-activity-in-chickpea-cicer-arietinum-l
#1
Zhankui Wang, Ming Chen, Yajing Zhu, Peipei Qian, Yali Zhou, Jiaping Wei, Yingzi Shen, Abudoukeyumu Mijiti, Aixing Gu, Zeng Wang, Hua Zhang, Hao Ma
Plant lectins are a group of highly diverse proteins that possess at least one non-catalytic domain that binds reversibly to a specific mono- or oligosaccharide. So far, only a few members in the lectin-arcelin-aAI1 supergene family in legume lectins have been reported to have inhibitory activity of α-amylases. A proteinaceous a-amylase inhibitor was isolated and purified using Ammonium sulfate precipitation (ASP), Ion exchange chromatography (IEC) and Reversed phase liquid chromatography (RPLC) from the mature seeds of chickpea...
July 11, 2017: Protein and Peptide Letters
https://www.readbyqxmd.com/read/29070546/impact-of-chronic-and-acute-inflammation-on-extra-and-intracellular-iron-homeostasis
#2
REVIEW
A Catharine Ross
Inflammation has a major impact on iron homeostasis. This review focuses on acute and chronic inflammation as it affects iron trafficking and, as a result, the availability of this essential micronutrient to the host. In situations of microbial infection, not only the host is affected but also the offending microorganisms, which, in general, not only require iron for their own growth but have evolved mechanisms to obtain it from the infected host. Key players in mammalian iron trafficking include several types of cells important to iron acquisition, homeostasis, and hematopoiesis (enterocytes, hepatocytes, macrophages, hematopoietic cells, and in the case of pregnancy, placental syncytiotrophoblast cells) and several forms of chaperone proteins, including, for nonheme iron, the transport protein transferrin and the intracellular iron-storage protein ferritin, and for heme iron, the chaperone proteins haptoglobin and hemopexin...
October 25, 2017: American Journal of Clinical Nutrition
https://www.readbyqxmd.com/read/29031784/isobaric-tags-for-relative-and-absolute-quantitation-itraq-based-proteomics-for-the-investigation-of-the-effect-of-huganqingzhi-on-non-alcoholic-fatty-liver-disease-in-rats
#3
Xiaorui Yao, Fan Xia, Waijiao Tang, Chunxin Xiao, Miaoting Yang, Benjie Zhou
ETHNOPHARMACOLOGICAL RELEVANCE: Hugan Qingzhi tablet (HQT), a traditional Chinese medicine formula has been adopted for preventing and treating nonalcoholic fatty liver disease (NAFLD). AIM: In order to explore the anti-NAFLD mechanisms of HQT, iTRAQ-based proteomic was employed to investigate the expression profiles of proteins in NAFLD rats induced by high-fat diet after HQT treatment. MATERIALS AND METHODS: The NAFLD rat model was administrated with high-fat diet (HFD) for 12weeks...
October 13, 2017: Journal of Ethnopharmacology
https://www.readbyqxmd.com/read/29029629/effects-of-age-and-gender-on-reference-levels-of-biomarkers-comprising-the-pediatric-renal-activity-index-for-lupus-nephritis-p-rail
#4
Michael R Bennett, Qing Ma, Jun Ying, Prasad Devarajan, Hermine Brunner
BACKGROUND: Systemic Lupus Erythematosus (SLE) is a multisystem autoimmune disease that disproportionately effects women and children of minorities. Renal involvement (lupus nephritis, or LN) occurs in up to 80% of children with SLE and is a major determinant of poor prognosis. We have developed a non-invasive pediatric Renal Activity Index for Lupus (p-RAIL) that consists of laboratory measures that reflect histologic LN activity. These markers are neutrophil gelatinase associated lipocalin (NGAL), kidney injury molecule-1 (KIM-1), monocyte chemotactic protein (MCP-1), adiponectin (APN), ceruloplasmin (CP) and hemopexin (HPX)...
October 13, 2017: Pediatric Rheumatology Online Journal
https://www.readbyqxmd.com/read/29016670/a-novel-function-of-n-linked-glycoproteins-alpha-2-hs-glycoprotein-and-hemopexin-implications-for-small-molecule-compound-mediated-neuroprotection
#5
Takuya Kanno, Kaori Yasutake, Kazunori Tanaka, Shinji Hadano, Joh-E Ikeda
Therapeutic agents to the central nervous system (CNS) need to be efficiently delivered to the target site of action at appropriate therapeutic levels. However, a limited number of effective drugs for the treatment of neurological diseases has been developed thus far. Further, the pharmacological mechanisms by which such therapeutic agents can protect neurons from cell death have not been fully understood. We have previously reported the novel small-molecule compound, 2-[mesityl(methyl)amino]-N-[4-(pyridin-2-yl)-1H-imidazol-2-yl] acetamide trihydrochloride (WN1316), as a unique neuroprotectant against oxidative injury and a highly promising remedy for the treatment of amyotrophic lateral sclerosis (ALS)...
2017: PloS One
https://www.readbyqxmd.com/read/28980221/cardiopulmonary-bypass-in-the-newborn-effects-of-circulatory-cell-free-hemoglobin-and-hyperoxia-evaluated-in-a-novel-rat-pup-model
#6
Åsa Jungner, Suvi Vallius, Matteo Bruschettini, Olga Romantsik, Magnus Gram, David Ley
BACKGROUND: Infants with congenital heart defects (CHD) are at risk for white matter brain injury. This novel rat pup model characterizes the systemic effects of intravasal cell-free hemoglobin and hyperoxia, hypothesizing that immature endogenous scavenging systems relate to increased vulnerability to conditions present during cardiopulmonary bypass (CPB). METHODS: Plasma pharmacokinetics of cell-free human hemoglobin (Hb) was determined after intraperitoneal (i...
October 4, 2017: Intensive Care Medicine Experimental
https://www.readbyqxmd.com/read/28974951/nutritional-immunity-triggers-the-modulation-of-iron-metabolism-genes-in-the-sub-antarctic-notothenioid-eleginops-maclovinus-in-response-to-piscirickettsia-salmonis
#7
Danixa Martínez, Ricardo Oyarzún, Juan Pablo Pontigo, Alex Romero, Alejandro J Yáñez, Luis Vargas-Chacoff
Iron deprivation is a nutritional immunity mechanism through which fish can limit the amount of iron available to invading bacteria. The aim of this study was to evaluate the modulation of iron metabolism genes in the liver and brain of sub-Antarctic notothenioid Eleginops maclovinus challenged with Piscirickettsia salmonis. The specimens were inoculated with two P. salmonis strains: LF-89 (ATCC(®) VR-1361™) and Austral-005 (antibiotic resistant). Hepatic and brain samples were collected at intervals over a period of 35 days...
2017: Frontiers in Immunology
https://www.readbyqxmd.com/read/28956007/insulin-attenuates-tnf%C3%AE-induced-hemopexin-mrna-an-anti-inflammatory-action-of-insulin-in-rat-h4iie-hepatoma-cells
#8
J Lee Franklin, William L Bennett, Joseph L Messina
Proinflammatory cytokines, including TNF-α and IL-6, can contribute to insulin resistance. Conversely, insulin has some actions that can be considered anti-inflammatory. Hemopexin is a Class 2 acute phase reactant and control of its transcription is predominantly regulated by IL-6, with TNF-α and IL-1β also inducing hemopexin gene expression. Thus, we asked whether insulin could inhibit the ability of TNF-α to stimulate hemopexin mRNA expression. In cultured rat hepatoma (H4IIE) cells, TNF-α significantly increased hemopexin mRNA accumulation...
March 2017: Biochemistry and Biophysics Reports
https://www.readbyqxmd.com/read/28945333/targeting-the-hemopexin-like-domain-of-latent-matrix-metalloproteinase-9-prommp-9-with-a-small-molecule-inhibitor-prevents-the-formation-of-focal-adhesion-junctions
#9
Vincent M Alford, Anushree Kamath, Xiaodong Ren, Kunal Kumar, Qianwen Gan, Monaf Awwa, Michael Tong, Markus A Seeliger, Jian Cao, Iwao Ojima, Nicole S Sampson
A lack of target specificity has greatly hindered the success of inhibitor development against matrix metalloproteinases (MMPs) for the treatment of various cancers. The MMP catalytic domains are highly conserved, whereas the hemopexin-like domains of MMPs are unique to each family member. The hemopexin-like domain of MMP-9 enhances cancer cell migration through self-interaction and heterointeractions with cell surface proteins including CD44 and α4β1 integrin. These interactions activate EGFR-MAP kinase dependent signaling that leads to cell migration...
November 17, 2017: ACS Chemical Biology
https://www.readbyqxmd.com/read/28927270/proteomic-analysis-of-peripheral-blood-mononuclear-cells-after-a-high-fat-high-carbohydrate-meal-with-orange-juice
#10
Daniela F S Chaves, Paulo C Carvalho, Elisa Brasili, Marcelo M Rogero, Neuza A Hassimotto, Jolene K Diedrich, James J Moresco, John R Yates, Franco M Lajolo
Oxidative stress and inflammation play a role in the physiopathology of insulin resistance, diabetes and cardiovascular disease. A single high-fat, high-carbohydrate (HFHC) meal induces an increase in inflammatory and oxidative stress markers in peripheral blood mononuclear cells (PBMC). Previous studies have shown that orange juice is able to prevent this response by inhibiting toll like receptors (TLR) expression and endotoxemia. Our goal was to study the proteome response in PBMC after the consumption of a HFHC meal consumed with water, orange juice or an isocaloric beverage (water with glucose)...
October 20, 2017: Journal of Proteome Research
https://www.readbyqxmd.com/read/28926834/heme-oxygenase-activity-and-heme-binding-in-a-neonatal-mouse-model
#11
Stephanie Kourula, Joyce Ang, Hui Zhao, Flora Kalish, Peter Vandenabeele, Karl G Sylvester, Ronald J Wong, David K Stevenson
BACKGROUND: Severe hemolytic disease of the newborn leads to the release of pro-oxidative free heme (FH). Heme oxygenase (HO) is primarily responsible for detoxifying FH. OBJECTIVE: To investigate the protective effects of HO in a model of heme overload. METHODS: For in vitro studies, NIH3T3 HO-1-luc cells were incubated with 10, 30, or 60 µM FH or methemalbumin (MHA). HO-1 promoter activity was assessed 3, 6, and 24 h after treatment. Cell survival was indexed by viability assays...
September 20, 2017: Neonatology
https://www.readbyqxmd.com/read/28914985/cherubism-mice-also-deficient-in-c-fos-exhibit-inflammatory-bone-destruction-executed-by-macrophages-that-express-mmp14-despite-the-absence-of-trap-osteoclasts
#12
Mizuho Kittaka, Kotoe Mayahara, Tomoyuki Mukai, Tetsuya Yoshimoto, Teruhito Yoshitaka, Jeffrey P Gorski, Yasuyoshi Ueki
Currently, it is believed that osteoclasts positive for tartrate-resistant acid phosphatase (TRAP+) are the exclusive bone-resorbing cells responsible for focal bone destruction in inflammatory arthritis. Recently, a mouse model of cherubism (Sh3bp2(KI/KI) ) with a homozygous gain-of-function mutation in the SH3-domain binding protein 2 (SH3BP2) was shown to develop auto-inflammatory joint destruction. Here, we demonstrate that Sh3bp2(KI/KI) mice also deficient in the FBJ osteosarcoma oncogene (c-Fos) still exhibit noticeable bone erosion at the distal tibia even in the absence of osteoclasts at 12 weeks old...
September 15, 2017: Journal of Bone and Mineral Research: the Official Journal of the American Society for Bone and Mineral Research
https://www.readbyqxmd.com/read/28888681/nano-reversed-phase-versus-nano-hydrophilic-interaction-liquid-chromatography-on-a-chip-in-the-analysis-of-hemopexin-glycopeptides
#13
Petr Kozlik, Miloslav Sanda, Radoslav Goldman
Analysis of the glycosylation of proteins is a challenge that requires orthogonal methods to achieve separation of the diverse glycoforms. A combination of reversed phase chromatography with tandem mass spectrometry (RP-LC-MS/MS) is one of the most powerful tools for glycopeptide analysis. In this work, we developed and compared RP-LC and hydrophilic interaction liquid chromatography (HILIC) in nanoscale on a chip combined with MS/MS in order to separate glycoforms of two peptides obtained from the tryptic digest of hemopexin...
October 13, 2017: Journal of Chromatography. A
https://www.readbyqxmd.com/read/28882792/hypothermal-stress-induced-differential-expression-profiles-of-the-immune-response-gene-warm-temperature-acclimation-associated-65-kda-protein-wap65-in-the-liver-of-fresh-water-and-seawater-milkfish-chanos-chanos
#14
Chia-Hao Chang, Jing-Yun Lin, Wan-Yu Lo, Tsung-Han Lee
The milkfish (Chanos chanos), an important aquaculture species, is intolerant to cold environments. Temperature fluctuations in the environment affect the physiological response, behavior, and survival rate of the fish. The warm-temperature-acclimation associated 65-kDa protein (Wap65) of teleosts was identified after heat shock treatment and has two isoforms. Both the isoforms were involved in the induction of immune responses in fish. They showed high degree of sequence conservation with the mammalian hemopexin and had high affinity for heme, which helped in the neutralization of free-heme and its transport to the liver...
September 4, 2017: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/28837209/erythrocyte-heme-proteins-and-stemi-implications-in-prognosis
#15
Judit Cubedo, Rosa Suades, Teresa Padro, Victoria Martin-Yuste, Manel Sabate-Tenas, Juan Cinca, Jordi Sans-Rosello, Alessandro Sionis, Lina Badimon
The role of erythrocytes in thrombus formation has been often neglected, but some studies have highlighted their active role in thrombotic events. Free-haemoglobin (Hb) has shown to induce oxidative-stress damage. Herein we have investigated the coordinated changes in heme-related proteins in patients with acute-coronary-syndromes (ACS), their association to ongoing thrombosis and their impact on patients' prognosis. The serum proteome of STEMI-patients (N=27) within the first 6h after event-onset and 3d after were compared to controls (N=60)...
October 5, 2017: Thrombosis and Haemostasis
https://www.readbyqxmd.com/read/28783684/il-22-scavenging-beyond-the-barrier
#16
REVIEW
Sandip K Datta
IL-22-induced hemopexin promotes nutritional immunity by scavenging iron from Citrobacter rodentium during systemic infection.
February 3, 2017: Science Immunology
https://www.readbyqxmd.com/read/28743114/cervical-alpha-actinin-4-is-upregulated-in-women-with-threatened-preterm-labor-and-microbial-invasion-of-the-amniotic-cavity
#17
Teresa Cobo, Montse Palacio, Maribel Grande, Ana B Sánchez-García, Josep M Estanyol, Marta López, Jordi Bosch, Cristina Martí, Eduard Gratacós
OBJECTIVE: To characterize the proteome profile of women with threatened preterm labor (PTL) below 34;0 weeks with and without microbial invasion of the amniotic cavity (MIAC) using mass spectrometry in the amniotic fluid (AF) and Western blot analysis in the cervical mucus and the vaginal fluid. SUBJECTS AND METHODS: In the discovery phase, a case-control study including 8 women with MIAC and 7 without matched for gestational age at sampling was performed. Proteomic profile characterization was done using the LTQ VELOS Orbitrap mass spectrometer in the AF...
July 26, 2017: Fetal Diagnosis and Therapy
https://www.readbyqxmd.com/read/28729288/urinary-extracellular-vesicles-of-podocyte-origin-and-renal-injury-in-preeclampsia
#18
Sarwat I Gilani, Ulrik Dolberg Anderson, Muthuvel Jayachandran, Tracey L Weissgerber, Ladan Zand, Wendy M White, Natasa Milic, Maria Lourdes Gonzalez Suarez, Rangit Reddy Vallapureddy, Åsa Nääv, Lena Erlandsson, John C Lieske, Joseph P Grande, Karl A Nath, Stefan R Hansson, Vesna D Garovic
Renal histologic expression of the podocyte-specific protein, nephrin, but not podocin, is reduced in preeclamptic compared with normotensive pregnancies. We hypothesized that renal expression of podocyte-specific proteins would be reflected in urinary extracellular vesicles (EVs) of podocyte origin and accompanied by increased urinary soluble nephrin levels (nephrinuria) in preeclampsia. We further postulated that podocyte injury and attendant formation of EVs are related mechanistically to cellfree fetal hemoglobin (HbF) in maternal plasma...
November 2017: Journal of the American Society of Nephrology: JASN
https://www.readbyqxmd.com/read/28683915/collagenolytic-matrix-metalloproteinase-structure-function-relationships-insights-from-molecular-dynamics-studies
#19
Tatyana G Karabencheva-Christova, Christo Z Christov, Gregg B Fields
Several members of the zinc-dependent matrix metalloproteinase (MMP) family catalyze collagen degradation. Experimental data reveal a collaboration between different MMP domains in order to achieve efficient collagenolysis. Molecular dynamics (MD) simulations have been utilized to provide atomistic details of the collagenolytic process. The triple-helical structure of collagen exhibits local regions of flexibility, with modulation of interchain salt bridges and water bridges contributing to accessibility of individual chains by the enzyme...
2017: Advances in Protein Chemistry and Structural Biology
https://www.readbyqxmd.com/read/28681077/direct-analysis-of-site-specific-n-glycopeptides-of-serological-proteins-in-dried-blood-spot-samples
#20
Na Young Choi, Heeyoun Hwang, Eun Sun Ji, Gun Wook Park, Ju Yeon Lee, Hyun Kyoung Lee, Jin Young Kim, Jong Shin Yoo
Dried blood spot (DBS) samples have a number of advantages, especially with respect to ease of collection, transportation, and storage and to reduce biohazard risk. N-glycosylation is a major post-translational modification of proteins in human blood that is related to a variety of biological functions, including metastasis, cell-cell interactions, inflammation, and immunization. Here, we directly analyzed tryptic N-glycopeptides from glycoproteins in DBS samples using liquid chromatography-tandem mass spectrometry (LC-MS/MS) without centrifugation of blood samples, depletion of major proteins, desalting of tryptic peptides, and enrichment of N-glycopeptides...
August 2017: Analytical and Bioanalytical Chemistry
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