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Hemopexin

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https://www.readbyqxmd.com/read/28173805/serum-proteomic-profiles-in-ckcs-with-mitral-valve-disease
#1
Chiara Locatelli, Cristian Piras, Giulia Riscazzi, Isabella Alloggio, Ilaria Spalla, Alessio Soggiu, Viviana Greco, Luigi Bonizzi, Paola Roncada, Paola G Brambilla
BACKGROUND: Myxomatous mitral valve disease (MVD) is the most common acquired heart disease in dogs, and the Cavalier King Charles Spaniel (CKCS) is the most studied breed because of the high prevalence, early onset and hereditary component evidenced in the breed. MVD has different severity levels, and there are many practical limitations in identifying its asymptomatic stages. Proteomic techniques are valuable for studying the proteins and peptides involved in cardiovascular diseases, including the period prior to the clinical onset of the disease...
February 7, 2017: BMC Veterinary Research
https://www.readbyqxmd.com/read/28152051/hemoglobin-induced-cell-trauma-indirectly-influences-endothelial-tlr9-activity-resulting-in-pulmonary-vascular-smooth-muscle-cell-activation
#2
Zoe Loomis, Paul Eigenberger, Katherine Redinius, Christina Lisk, Vijaya Karoor, Eva Nozik-Grayck, Scott K Ferguson, Kathryn Hassell, Rachelle Nuss, Kurt Stenmark, Paul Buehler, David C Irwin
It is now well established that both inherited and acquired forms of hemolytic disease can promote pulmonary vascular disease consequent of free hemoglobin (Hb) induced NO scavenging, elevations in reactive oxygen species and lipid peroxidation. It has recently been reported that oxidative stress can activate NFkB through a toll-like receptor 9 (TLR9) mediated pathway; further, TLR9 can be activated by either nuclear or mitochondrial DNA liberated by stress induced cellular trauma. We hypothesis that Hb induced lipid peroxidation and subsequent endothelial cell trauma is linked to TLR9 activation, resulting in IL-6 mediated pulmonary smooth muscle cell proliferation...
2017: PloS One
https://www.readbyqxmd.com/read/28143953/hemolytic-anemia-repressed-hepcidin-level-without-hepatocyte-iron-overload-lesson-from-g%C3%A3-nther-disease-model
#3
Sarah Millot, Constance Delaby, Boualem Moulouel, Thibaud Lefebvre, Nathalie Pilard, Nicolas Ducrot, Cécile Ged, Philippe Lettéron, Lucia de Franceschi, Jean Charles Deybach, Carole Beaumont, Laurent Gouya, Hubert De Verneuil, Saïd Lyoumi, Hervé Puy, Zoubida Karim
Hemolysis occurring in hematologic diseases is often associated with an iron loading anemia. This iron overload is the result of a massive outflow of hemoglobin into the bloodstream, but the mechanism of hemoglobin handling has not been fully elucidated. Here, in a congenital erythropoietic porphyria mouse model, we evaluate the impact of hemolysis and regenerative anemia on hepcidin synthesis and iron metabolism. Hemolysis was confirmed by a complete drop in haptoglobin, hemopexin and increased plasma lactate dehydrogenase, an increased red blood cell distribution width and osmotic fragility, a reduced half-life of red blood cells, and increased expression of heme oxygenase 1...
February 2017: Haematologica
https://www.readbyqxmd.com/read/28142234/plasma-protein-binding-structure-activity-relationships-related-to-the-n-terminus-of-daptomycin
#4
Elena K Schneider, Johnny X Huang, Vincenzo Carbone, Meiling Han, Yan Zhu, Sue Nang, Keith K Khoo, Johnson Mak, Matthew A Cooper, Jian Li, Tony Velkov
Daptomycin is a lipopeptide antibiotic that is highly bound to plasma proteins. To date, the plasma components and structure-activity relationships responsible for the plasma protein binding profile of daptomycin remain uncharacterized. In the present study we have employed a surface plasmon resonance assay together with molecular docking techniques to investigate the plasma protein binding structure-activity relationships related to the N-terminal fatty acyl of daptomycin. Three compounds were investigated: (1) native daptomycin, which displays an N-terminal n-decanoyl fatty acid side chain, and two analogues with modifications to the N-terminal fatty acyl chain; (2) des-acyl daptomycin; and (3) acetyl-daptomycin...
February 10, 2017: ACS Infectious Diseases
https://www.readbyqxmd.com/read/28119529/physiologic-and-genetic-evidence-links-hemopexin-to-triglycerides-in-mice-and-humans
#5
H A Lawson, M Zayed, J P Wayhart, E Fabbrini, L Love-Gregory, S Klein, C F Semenkovich
BACKGROUND/OBJECTIVES: Elevated triglycerides predict insulin resistance and vascular disease in obesity, but how the inert triglyceride molecule is related to development of metabolic disease is unknown. To pursue novel potential mediators of triglyceride-associated metabolic disease, we used a forward genetics approach involving inbred mice and translated our findings to human subjects. SUBJECTS/METHODS: Hemopexin was identified as a differentially expressed gene within a quantitative trait locus associated with serum triglycerides in an F16 advanced intercross between the LG/J and SM/J strains of mice...
January 25, 2017: International Journal of Obesity: Journal of the International Association for the Study of Obesity
https://www.readbyqxmd.com/read/28034890/a-novel-mechanism-regulating-human-platelet-activation-by-mmp-2-mediated-par1-biased-signaling
#6
Manuela Sebastiano, Stefania Momi, Emanuela Falcinelli, Loredana Bury, Marc Hoylaerts, Paolo Gresele
Platelets contain and release several matrix metalloproteinases (MMPs). Among these, active MMP-2 enhances platelet aggregation by favouring the activation of phosphatidyl-inositol-3- kinase (PI3-K) and contributes to arterial thrombosis. The platelet surface target of MMP-2 and the mechanism through which it primes platelets to respond to subsequent stimuli are still unknown. We show that active MMP-2 enhances platelet activation induced by weak stimuli by cleaving PAR1 at a non-canonical extracellular site different from the thrombin-cleavage site, and thus starts biased receptor signaling triggering only some of the signaling pathways normally activated by full PAR1 agonism...
December 29, 2016: Blood
https://www.readbyqxmd.com/read/28030762/placental-proteomics-provides-insights-into-pathophysiology-of-pre-eclampsia-and-predicts-possible-markers-in-plasma
#7
Sheon Mary, Mahesh J Kulkarni, Dipankar Malakar, Sadhana R Joshi, Savita S Mehendale, Ashok P Giri
Pre-eclampsia is a hypertensive disorder characterized by the new onset of hypertension >140/90 mmHg and proteinuria after the 20th week of gestation. The disorder is multifactorial and originates with abnormal placentation. Comparison of the placental proteome of normotensive (n = 25) and pre-eclamptic (n = 25) patients by gel-free proteomic techniques identified a total of 2145 proteins in the placenta of which 180 were differentially expressed (>1.3 fold, p < 0.05). Gene ontology enrichment analysis of biological process suggested that the differentially expressed proteins belonged to various physiological processes such as angiogenesis, apoptosis, oxidative stress, hypoxia, and placental development, which are implicated in the pathophysiology of pre-eclampsia...
January 26, 2017: Journal of Proteome Research
https://www.readbyqxmd.com/read/27864463/increased-brain-hemopexin-levels-improve-outcomes-after-intracerebral-hemorrhage
#8
Jenna L Leclerc, Juan Santiago-Moreno, Alex Dang, Andrew S Lampert, Pedro E Cruz, Awilda M Rosario, Todd E Golde, Sylvain Doré
Following intracerebral hemorrhage (ICH), extracellular heme precipitates secondary brain injury, which results in irreversible brain damage and enduring neurological deficits. Hemopexin (Hpx) is an endogenous protein responsible for scavenging heme, thereby modulating its intrinsic proxidant/proinflammatory properties. Although Hpx is present in the brain, the endogenous levels are insufficient to combat the massive heme overload following ICH. We hypothesized that increasing brain Hpx levels would improve ICH outcomes...
November 18, 2016: Journal of Cerebral Blood Flow and Metabolism
https://www.readbyqxmd.com/read/27768920/nano-lc-ms-ms-for-the-identification-of-proteins-trapped-in-sorbent-cartridges-used-for-coupled-plasma-filtration-adsorption-treatments-of-healthy-pigs
#9
Donatella Nardiello, Clelia Prattichizzo, Maria Teresa Rocchetti, Loreto Gesualdo, Diego Centonze
A dedicated proteomic approach based on nano-Liquid Chromatography coupled with tandem mass spectrometry in ion trap is proposed for the analysis of proteins trapped in sorbent resin cartridges used to remove inflammatory mediators from blood by coupled plasma filtration adsorption (CPFA). The final purpose of the proposed proteomic approach was to obtain a reference map of plasma proteins trapped in CPFA sorbents used for the extracorporeal blood purification of healthy pigs, with the potential impact to design new bio-filters able to control the inflammatory imbalance under pathological conditions, such as severe sepsis...
January 5, 2017: Journal of Pharmaceutical and Biomedical Analysis
https://www.readbyqxmd.com/read/27754420/effects-of-mutations-on-structure-function-relationships-of-matrix-metalloproteinase-1
#10
Warispreet Singh, Gregg B Fields, Christo Z Christov, Tatyana G Karabencheva-Christova
Matrix metalloproteinase-1 (MMP-1) is one of the most widely studied enzymes involved in collagen degradation. Mutations of specific residues in the MMP-1 hemopexin-like (HPX) domain have been shown to modulate activity of the MMP-1 catalytic (CAT) domain. In order to reveal the structural and conformational effects of such mutations, a molecular dynamics (MD) study was performed of in silico mutated residues in the X-ray crystallographic structure of MMP-1 complexed with a collagen-model triple-helical peptide (THP)...
October 14, 2016: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/27716784/the-mechanism-of-proinflammatory-hdl-generation-in-sickle-cell-disease-is-linked-to-cell-free-hemoglobin-via-haptoglobin
#11
Xiang Ji, Yimin Feng, Hui Tian, Wei Meng, Weiling Wang, Na Liu, Jun Zhang, Lingshu Wang, Jian Wang, Haiqing Gao
In sickle cell disease (SCD), the inflammatory properties of high-density lipoprotein (HDL) can be changed by cell-free hemoglobin (Hb), which is released into the blood during hemolysis. Hb in the plasma of SCD patients or mice can bind with HDL specifically inducing an inflammatory reaction. In our study, we found increased amounts of inflammatory factor proteins in the chronic oxidative state of SCD with higher levels of Hb, haptoglobin (Hp) and hemopexin (Hx) in the apolipoprotein A-I (ApoA-1) particles of HDL and the role of HDL is changed from being anti-inflammatory to proinflammatory...
2016: PloS One
https://www.readbyqxmd.com/read/27688741/increased-sialylation-of-site-specific-o-glycoforms-of-hemopexin-in-liver-disease
#12
Miloslav Sanda, Julius Benicky, Jing Wu, Yiwen Wang, Kepher Makambi, Jaeil Ahn, Coleman I Smith, Peng Zhao, Lihua Zhang, Radoslav Goldman
BACKGROUND: Non-invasive monitoring of liver disease remains an important health issue. Liver secreted glycoproteins reflect pathophysiological states of the organ and represent a rational target for serologic monitoring. In this study, we describe sialylated O-glycoforms of liver-secreted hemopexin (HPX) and quantify them as a ratio of disialylated to monosialylated form (S-HPX). METHODS: We measured S-HPX in serum of participants of the HALT-C trial using a LC-MS/MS-MRM assay...
2016: Clinical Proteomics
https://www.readbyqxmd.com/read/27682196/the-plasma-protein-binding-proteome-of-ertapenem-a-novel-compound-centric-proteomic-approach-for-elucidating-drug-plasma-protein-binding-interactions
#13
Mark A Baker, Elena K Schneider, Johnny X Huang, Matthew A Cooper, Jian Li, Tony Velkov
Ertapenem is an important first-line carbapenem antibiotic used for the treatment of aerobic Gram-negative bacterial infections. It is the only marketed carbapenem that is highly bound to plasma proteins and displays a concentration-dependent and saturable plasma protein binding profile. To date, the plasma components responsible for sequestering ertapenems antibacterial activity remain uncharacterized. In the present study, we have employed an orthogonal, multiplatform approach, including novel compound-centric displacement proteomics and surface plasmon resonance to characterize the plasma protein binding proteome of ertapenem...
December 16, 2016: ACS Chemical Biology
https://www.readbyqxmd.com/read/27664937/comparative-proteome-analysis-of-egg-yolk-plasma-proteins-during-storage
#14
Dan Gao, Ning Qiu, Yaping Liu, Meihu Ma
BACKGROUND: Physical changes such as chicken egg white thinning and flattening of egg yolk occur during storage, implying the decline of the egg qualities. To reveal the deteriorative process related to the chicken egg internal qualities, comparative proteomic method was used in this study to analyze the alterations of egg yolk plasma proteins at different storage times (0, 20, and 40 days) under ambient temperature of 22 ± 2 °C. RESULT: Using two-dimensional electrophoresis (2-DE) followed by MALDI-TOF MS/MS, 33 protein spots representing 12 proteins were identified with significant (p ˂ 0...
September 24, 2016: Journal of the Science of Food and Agriculture
https://www.readbyqxmd.com/read/27646713/changes-in-the-expression-of-n-and-o-glycopeptides-in-patients-with-colorectal-cancer-and-hepatocellular-carcinoma-quantified-by-full-ms-scan-ft-icr-and-multiple-reaction-monitoring
#15
Petra Darebna, Petr Novak, Radek Kucera, Ondrej Topolcan, Miloslav Sanda, Radoslav Goldman, Petr Pompach
: Alternations in the glycosylation of proteins have been described in connection with several cancers, including hepatocellular carcinoma (HCC) and colorectal cancer. Analytical tools, which use combination of liquid chromatography and mass spectrometry, allow precise and sensitive description of these changes. In this study, we use MRM and FT-ICR operating in full-MS scan, to determine ratios of intensities of specific glycopeptides in HCC, colorectal cancer, and liver metastasis of colorectal cancer...
September 16, 2016: Journal of Proteomics
https://www.readbyqxmd.com/read/27646125/changes-in-serum-proteins-after-endotoxin-administration-in-healthy-and-choline-treated-calves
#16
RANDOMIZED CONTROLLED TRIAL
Z Yilmaz, O Eralp Inan, M Kocaturk, A T Baykal, O Hacariz, I Hatipoglu, A Tvarijonaviciute, M Cansev, J Ceron, I H Ulus
BACKGROUND: This study aimed to investigate the possible serum protein changes after endotoxin administration in healthy and choline-treated calves using proteomics. These results are expected to contribute to the understanding of the pathophysiological mechanisms of endotoxemia and the beneficial effect of choline administration in this clinical situation. METHODS: Healthy-calves (n = 20) were divided into 4 groups: Control, Choline treated (C), Lipopolysaccharide administered (LPS), and LPS + C...
September 20, 2016: BMC Veterinary Research
https://www.readbyqxmd.com/read/27642551/differential-heme-release-from-various-hemoglobin-redox-states-and-the-upregulation-of-cellular-heme-oxygenase-1
#17
Tigist Kassa, Sirsendu Jana, Fantao Meng, Abdu I Alayash
Despite advances in our understanding of the oxidative pathways mediated by free hemoglobin (Hb), the precise contribution of its highly reactive redox forms to tissue and organ toxicities remains ambiguous. Heme, a key degradation byproduct of Hb oxidation, has recently been recognized as a damage-associated molecular pattern (DAMP) molecule, able to trigger inflammatory responses. Equally damaging is the interaction of the highly redox active forms of Hb with other biological molecules. We determined the kinetics of heme loss from individual Hb redox states-ferrous (Fe(2+)), ferric (Fe(3+)), and ferryl (Fe(4+))-using two different heme receptor proteins: hemopexin (Hxp), a naturally occurring heme scavenger in plasma, and a double mutant (H64Y/V86F), apomyoglobin (ApoMb), which avidly binds heme released from Hb...
September 2016: FEBS Open Bio
https://www.readbyqxmd.com/read/27602154/proteomic-profile-of-saliva-and-plasma-from-women-with-impalpable-breast-lesions
#18
Lucas Delmonico, Maryah Bravo, Rafaele Tavares Silvestre, Maria Helena Faria Ornellas, Carolina Maria De Azevedo, Gilda Alves
The present study evaluated the proteomic profile of saliva and plasma from women with impalpable breast lesions using nano-liquid chromatography-quadrupole-time-of-flight (nLC-Q-TOF) technology. Plasma and saliva from patients with fibroadenoma (n=10), infiltrating ductal carcinoma (n=10) and healthy control groups (n=8) were assessed by combinations of inter/intra-group analyses, revealing significant quantitative and qualitative differences. The major differentially-expressed proteins in the saliva of patients compared with the controls were α2-macroglobulin and ceruloplasmin, but the proteins that met the minimum fold-change and P-value cut-offs were leukocyte elastase inhibitor and α-enolase, and deleted in malignant brain tumors 1...
September 2016: Oncology Letters
https://www.readbyqxmd.com/read/27566655/meningiomas-and-proteomics-focus-on-new-potential-biomarkers-and-molecular-pathways
#19
REVIEW
Rosaria Viola Abbritti, Francesca Polito, Maria Cucinotta, Claudio Lo Giudice, Maria Caffo, Chiara Tomasello, Antonino Germanò, Mohammed Aguennouz
Meningiomas are one of the most common tumors affecting the central nervous system, exhibiting a great heterogeneity in grading, treatment and molecular background. This article provides an overview of the current literature regarding the molecular aspect of meningiomas. Analysis of potential biomarkers in serum, cerebrospinal fluid (CSF) and pathological tissues was reported. Applying bioinformatic methods and matching the common proteic profile, arising from different biological samples, we highlighted the role of nine proteins, particularly related to tumorigenesis and grading of meningiomas: serpin peptidase inhibitor alpha 1, ceruloplasmin, hemopexin, albumin, C3, apolipoprotein, haptoglobin, amyloid-P-component serum and alpha-1-beta-glycoprotein...
September 2016: Cancer Genomics & Proteomics
https://www.readbyqxmd.com/read/27515862/alterations-in-systemic-extracellular-heme-and-hemopexin-are-associated-with-adverse-clinical-outcomes-in-ugandan-children-with-severe-malaria
#20
Robyn E Elphinstone, Andrea L Conroy, Michael Hawkes, Laura Hermann, Sophie Namasopo, H Shaw Warren, Chandy C John, W Conrad Liles, Kevin C Kain
BACKGROUND: Malaria remains a major cause of global mortality. Extracellular heme, released during malaria-induced hemolysis, mediates a number of pathogenic processes associated with vascular and organ injury. Hemopexin (hpx) facilitates the degradation of extracellular heme. In this study, we explore the hypothesis that dysregulation of the heme-hpx axis is associated with disease severity, acute kidney injury (AKI), and outcome. METHODS: Plasma levels of hemin and hpx (at admission, day 3, and day 14) were assessed in children with severe malaria in Jinja, Uganda...
October 15, 2016: Journal of Infectious Diseases
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