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Hemopexin

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https://www.readbyqxmd.com/read/28620062/urine-biomarkers-to-predict-response-to-lupus-nephritis-therapy-in-children-and-young-adults
#1
Hermine I Brunner, Michael R Bennett, Gaurav Gulati, Khalid Abulaban, Marisa S Klein-Gitelman, Stacy P Ardoin, Lori B Tucker, Kelly A Rouster-Stevens, David Witte, Jun Ying, Prasad Devarajan
OBJECTIVE: To delineate urine biomarkers that forecast response to therapy of lupus nephritis (LN). METHODS: Starting from the time of kidney biopsy, patients with childhood-onset systemic lupus erythematosus who were diagnosed with LN were studied serially. Levels of 15 biomarkers were measured in random spot urine samples, including adiponectin, α-1-acid glycoprotein (AGP), ceruloplasmin, hemopexin, hepcidin, kidney injury molecule 1, monocyte chemotactic protein-1, lipocalin-like prostaglandin D synthase (LPGDS), transforming growth factor-β (TGF-β), transferrin, and vitamin D binding protein (VDBP)...
June 15, 2017: Journal of Rheumatology
https://www.readbyqxmd.com/read/28596380/identification-of-oxidative-modifications-of-hemopexin-and-their-predicted-physiological-relevance
#2
Peter Hahl, Rachel Hunt, Edward S Bjes, Andrew Skaff, J Andrew Keightley, Ann Smith
Hemopexin protects against heme toxicity in hemolytic diseases and conditions, sepsis and sickle cell disease. This protection is sustained by heme-hemopexin complexes present in biological fluids that resist oxidative damage during heme-driven inflammation. However, apo-hemopexin is vulnerable to inactivation by reactive nitrogen and oxygen species that covalently modify amino acids. The consequent nitration of amino acids is considered a selective and specific effect reflecting biological events. Using liquid chromatography-tandem mass spectrometry, we discovered low endogenous levels of tyrosine nitration in the peptide YYCFQGNQFLR of human hemopexin, which was similarly nitrated in rabbit and rat hemopexins...
June 8, 2017: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/28561948/identification-of-fucosylated-fetuin-a-as-a-potential-biomarker-for-cholangiocarcinoma
#3
Lucy Betesh, Mary Ann Comunale, Mengjun Wang, Hongyan Liang, Julie Hafner, Aykan Karabudak, Nasra H Giama, Catherine D Moser, Eiji Miyoshi, Lewis R Roberts, Timothy M Block, Anand Mehta
CLINICAL RELEVANCE: Cholangiocarcinoma is a cancer of bile duct cells for which no effective biomarker exists. Tumor masses are often not identifiable by imaging modalities as a result of the location of the tumor in the hilum of the liver or within the bile ducts. Because of the problems with obtaining a definitive tissue diagnosis, treatment and management decisions for patients with biliary strictures that may be malignant are difficult. The work presented here highlights the potential role of fucosylated fetuin-A as a biomarker of cholangiocarcinoma and may help in the early detection of this deadly cancer...
May 31, 2017: Proteomics. Clinical Applications
https://www.readbyqxmd.com/read/28560284/data-demonstrating-the-anti-oxidant-role-of-hemopexin-in-the-heart
#4
Giada Ingoglia, Can Martin Sag, Nikolai Rex, Lucia De Franceschi, Francesca Vinchi, James Cimino, Sara Petrillo, Stefan Wagner, Klaus Kreitmeier, Lorenzo Silengo, Fiorella Altruda, Lars S Maier, Emilio Hirsch, Alessandra Ghigo, Emanuela Tolosano
The data presented in this article are related to the research article entitled Hemopexin counteracts systolic dysfunction induced by heme-driven oxidative stress (G. Ingoglia, C. M. Sag, N. Rex, L. De Franceschi, F. Vinchi, J. Cimino, S. Petrillo, S. Wagner, K. Kreitmeier, L. Silengo, F. Altruda, L. S. Maier, E. Hirsch, A. Ghigo and E. Tolosano, 2017) [1]. Data show that heme induces reactive oxygen species (ROS) production in primary cardiomyocytes. H9c2 myoblastic cells treated with heme bound to human Hemopexin (Hx) are protected from heme accumulation and oxidative stress...
August 2017: Data in Brief
https://www.readbyqxmd.com/read/28550901/protective-effect-of-hemopexin-on-systemic-inflammation-and-acute-lung-injury-in-an-endotoxemia-model
#5
Jae Yun Jung, Young Ho Kwak, Ikwan Chang, Woon Yong Kwon, Gil Joon Suh, Dongyoul Choi
BACKGROUND: Hemopexin (HPX) has been identified as an anti-inflammatory agent, but its role in endotoxemia is unclear. The purpose of this study was to determine whether HPX suppresses systemic and lung inflammation in a mouse model of endotoxemia. MATERIALS AND METHODS: At 30 min of intraperitoneal administration of lipopolysaccharide (LPS; 10 mg/kg), either distilled water (LPS-only treated animals) or HPX (5 mg/kg) was injected into mice via the tail vein, and the survival rates were analyzed after 36 h...
May 15, 2017: Journal of Surgical Research
https://www.readbyqxmd.com/read/28502635/identification-of-vitreous-proteins-in-retinopathy-of-prematurity
#6
Koji Sugioka, Akio Saito, Shunji Kusaka, Kazuki Kuniyoshi, Yoshikazu Shimomura
Retinopathy of prematurity (ROP) is a disorder of blood vessels in the retina developed in premature infants and the leading cause of the blindness in children. Proteomic analysis was performed to identify vitreous proteins specific to patients with ROP. Vitreous humor samples were obtained from three patients with ROP and two patients with congenital cataract, the latter included as a control group. The vitreous samples were separated by 2D-PAGE and the proteins running as definitive spots were identified by MALDI-TOF MS spectrometry...
May 11, 2017: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/28469399/a-novel-biomarker-panel-to-identify-steroid-resistance-in-childhood-idiopathic-nephrotic-syndrome
#7
Michael R Bennett, LaTawnya Pleasant, Christopher Haffner, Qing Ma, Wendy D Haffey, Jun Ying, Michael Wagner, Kenneth D Greis, Prasad Devarajan
Idiopathic nephrotic syndrome (NS) is the most common glomerular disorder of childhood. Response to initial treatment with corticosteroids is an indicator of prognosis, as resistant patients often present more progressive disease. In this cross-sectional pilot study, we set out to discover a panel of noninvasive biomarkers that could distinguish steroid-resistant nephrotic syndrome (SRNS) from steroid-sensitive nephrotic syndrome (SSNS). Information gleaned from such a panel could yield more individualized treatment plans and prevent unnecessary steroid exposure in patients unlikely to respond...
2017: Biomarker Insights
https://www.readbyqxmd.com/read/28444931/study-of-structure-dependent-chromatographic-behavior-of-glycopeptides-using-reversed-phase-nanolc
#8
Petr Kozlik, Radoslav Goldman, Miloslav Sanda
Analysis of glycosylation is challenging due to micro- and macro-heterogeneity of the protein attachment. A combination of liquid chromatography with tandem mass spectrometry is one of the most powerful tools for glycopeptide analysis. In this work, we show the effect of various monosaccharide units on the retention time of glycopeptides. Retention behavior of several glycoforms of six peptides obtained from tryptic digest of haptoglobin, hemopexin and sex hormone binding globulin was studied on a reversed phase chromatographic column...
April 26, 2017: Electrophoresis
https://www.readbyqxmd.com/read/28442379/peripheral-membrane-associations-of-matrix-metalloproteinases
#9
REVIEW
Steven R Van Doren, Tara C Marcink, Rama K Koppisetti, Alexander Jurkevich, Yan G Fulcher
Water soluble matrix metalloproteinases (MMPs) have been regarded as diffusing freely in the extracellular matrix. Yet multiple MMPs are also observed at cell surfaces. Their membrane-proximal activities include sheddase activities, collagenolysis, bacterial killing, and intracellular trafficking reaching as far as the nucleus. The catalytic domains of MMP-7 and MMP-12 bind bilayers peripherally, each in two different orientations, by presenting positive charges and a few hydrophobic groups to the surface. Related peripheral membrane associations are predicted for other soluble MMPs...
April 23, 2017: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/28436274/depletion-of-hdl3-high-density-lipoprotein-and-altered-functionality-of-hdl2-in-blood-from-sickle-cell-patients
#10
Eric Soupene, Sandra K Larkin, Frans A Kuypers
In sickle cell disease (SCD), alterations of cholesterol metabolism is in part related to abnormal levels and activity of plasma proteins such as lecithin cholesterol acyltransferase (LCAT), and apolipoprotein A-I (ApoA-I). In addition, the size distribution of ApoA-I high density lipoproteins (HDL) differs from normal blood. The ratio of the amount of HDL2 particle relative to the smaller higher density pre-β HDL (HDL3) particle was shifted toward HDL2. This lipoprotein imbalance is exacerbated during acute vaso-occlusive episodes (VOE) as the relative levels of HDL3 decrease...
January 1, 2017: Experimental Biology and Medicine
https://www.readbyqxmd.com/read/28420988/heme-as-a-target-for-therapeutic-interventions
#11
REVIEW
Stephan Immenschuh, Vijith Vijayan, Sabina Janciauskiene, Faikah Gueler
Heme is a complex of iron and the tetrapyrrole protoporphyrin IX with essential functions in aerobic organisms. Heme is the prosthetic group of hemoproteins such as hemoglobin and myoglobin, which are crucial for reversible oxygen binding and transport. By contrast, high levels of free heme, which may occur in various pathophysiological conditions, are toxic via pro-oxidant, pro-inflammatory and cytotoxic effects. The toxicity of heme plays a major role for the pathogenesis of prototypical hemolytic disorders including sickle cell disease and malaria...
2017: Frontiers in Pharmacology
https://www.readbyqxmd.com/read/28413025/biochemical-and-biological-attributes-of-matrix-metalloproteinases
#12
Ning Cui, Min Hu, Raouf A Khalil
Matrix metalloproteinases (MMPs) are a family of zinc-dependent endopeptidases that are involved in the degradation of various proteins in the extracellular matrix (ECM). Typically, MMPs have a propeptide sequence, a catalytic metalloproteinase domain with catalytic zinc, a hinge region or linker peptide, and a hemopexin domain. MMPs are commonly classified on the basis of their substrates and the organization of their structural domains into collagenases, gelatinases, stromelysins, matrilysins, membrane-type (MT)-MMPs, and other MMPs...
2017: Progress in Molecular Biology and Translational Science
https://www.readbyqxmd.com/read/28400318/hemopexin-counteracts-systolic-dysfunction-induced-by-heme-driven-oxidative-stress
#13
Giada Ingoglia, Can Martin Sag, Nikolai Rex, Lucia De Franceschi, Francesca Vinchi, James Cimino, Sara Petrillo, Stefan Wagner, Klaus Kreitmeier, Lorenzo Silengo, Fiorella Altruda, Lars S Maier, Emilio Hirsch, Alessandra Ghigo, Emanuela Tolosano
Heart failure is a leading cause of morbidity and mortality in patients affected by different disorders associated to intravascular hemolysis. The leading factor is the presence of pathologic amount of pro-oxidant free heme in the bloodstream, due to the exhaustion of the natural heme scavenger Hemopexin (Hx). Here, we evaluated whether free heme directly affects cardiac function, and tested the therapeutic potential of replenishing serum Hx for increasing serum heme buffering capacity. The effect of heme on cardiac function was assessed in vitro, on primary cardiomyocytes and H9c2 myoblast cell line, and in vivo, in Hx(-/-) mice and in genetic and acquired mouse models of intravascular hemolysis...
April 8, 2017: Free Radical Biology & Medicine
https://www.readbyqxmd.com/read/28389660/characteristics-of-the-heme-catabolic-pathway-in-mild-unconjugated-hyperbilirubinemia-and-their-associations-with-inflammation-and-disease-prevention
#14
Christine Mölzer, Marlies Wallner, Carina Kern, Anela Tosevska, René Zadnikar, Daniel Doberer, Rodrig Marculescu, Karl-Heinz Wagner
Heme catabolism exerts physiological functions that impact health through depressing inflammation. Upon reactive pathway progression, as in Gilbert's Syndrome (GS; UGT1A1*28 polymorphism), aggravated health effects have been determined. Based on lower inflammation and improved metabolic health reported for GS, inter-group differences in heme catabolism were explored. Therefore, a case-control study including 120 fasted, healthy, age- and gender matched subjects with/without GS, was conducted. Genetic expressions of HMOX-1 and BLVRA were measured...
April 7, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28314763/endothelial-dysfunction-inhibits-the-ability-of-haptoglobin-to-prevent-hemoglobin-induced-hypertension
#15
Jan A Graw, Binglan Yu, Emanuele Rezoagli, H Shaw Warren, Emmanuel S Buys, Donald B Bloch, Warren M Zapol
BACKGROUND: Intravascular hemolysis produces injury in a variety of human diseases including hemoglobinopathies, malaria, and sepsis. The adverse effects of increased plasma hemoglobin are partly mediated by depletion of nitric oxide (NO) and result in vasoconstriction. Circulating plasma proteins haptoglobin and hemopexin scavenge extracellular hemoglobin and cell-free heme, respectively. METHODS: The ability of human haptoglobin or hemopexin to inhibit the adverse effects of NO-scavenging by circulating murine hemoglobin was tested in C57Bl/6 mice...
March 17, 2017: American Journal of Physiology. Heart and Circulatory Physiology
https://www.readbyqxmd.com/read/28299732/structural-studies-of-matrix-metalloproteinase-by-x-ray-diffraction
#16
Elena Decaneto, Wolfgang Lubitz, Hideaki Ogata
Matrix Metalloproteinases (MMPs) are a family of proteolytic enzymes whose endopeptidase activity is dependent on the presence of specific metal ions. MT1-MMP (or MMP-14), which has been implicated in tumor progression and cellular invasion, contains a membrane-spanning region located C-terminal to a hemopexin-like domain and an N-terminal catalytic domain. We recombinantly expressed the catalytic domain of human MT1-MMP in E. coli and purified it from inclusion bodies using a refolding protocol that yielded significant quantities of active protein...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28286877/il-22-controls-iron-dependent-nutritional-immunity-against-systemic-bacterial-infections
#17
Kei Sakamoto, Yun-Gi Kim, Hideki Hara, Nobuhiko Kamada, Gustavo Caballero-Flores, Emanuela Tolosano, Miguel P Soares, José L Puente, Naohiro Inohara, Gabriel Núñez
Host immunity limits iron availability to pathogenic bacteria, but whether immunity limits pathogenic bacteria from accessing host heme, the major source of iron in the body, remains unclear. Using Citrobacter rodentium, a mouse enteric pathogen and Escherichia coli, a major cause of sepsis in humans as models, we find that interleukin-22, a cytokine best known for its ability to promote epithelial barrier function, also suppresses the systemic growth of bacteria by limiting iron availability to the pathogen...
February 2017: Science Immunology
https://www.readbyqxmd.com/read/28242648/identification-of-novel-chondroprotective-mediators-in-resolving-inflammatory-exudates
#18
Magdalena K Kaneva, Karin V Greco, Sarah E Headland, Trinidad Montero-Melendez, Prashant Mori, Kevin Greenslade, Costantino Pitzalis, Adrian Moore, Mauro Perretti
We hypothesized that exudates collected at the beginning of the resolution phase of inflammation might be enriched for tissue protective molecules; thus an integrated cellular and molecular approach was applied to identify novel chondroprotective bioactions. Exudates were collected 6 h (inflammatory) and 24 h (resolving) following carrageenan-induced pleurisy in rats. The resolving exudate was subjected to gel filtration chromatography followed by proteomics, identifying 61 proteins. Fractions were added to C28/I2 chondrocytes, grown in micromasses, ions with or without IL-1β or osteoarthritic synovial fluids for 48 h...
April 1, 2017: Journal of Immunology: Official Journal of the American Association of Immunologists
https://www.readbyqxmd.com/read/28238792/an-all-recombinant-protein-based-culture-system-specifically-identifies-hematopoietic-stem-cell-maintenance-factors
#19
Aki Ieyasu, Reiko Ishida, Takaharu Kimura, Maiko Morita, Adam C Wilkinson, Kazuhiro Sudo, Toshinobu Nishimura, Jun Ohehara, Yoko Tajima, Chen-Yi Lai, Makoto Otsu, Yukio Nakamura, Hideo Ema, Hiromitsu Nakauchi, Satoshi Yamazaki
Hematopoietic stem cells (HSCs) are considered one of the most promising therapeutic targets for the treatment of various blood disorders. However, due to difficulties in establishing stable maintenance and expansion of HSCs in vitro, their insufficient supply is a major constraint to transplantation studies. To solve these problems we have developed a fully defined, all-recombinant protein-based culture system. Through this system, we have identified hemopexin (HPX) and interleukin-1α as responsible for HSC maintenance in vitro...
March 14, 2017: Stem Cell Reports
https://www.readbyqxmd.com/read/28173805/serum-proteomic-profiles-in-ckcs-with-mitral-valve-disease
#20
Chiara Locatelli, Cristian Piras, Giulia Riscazzi, Isabella Alloggio, Ilaria Spalla, Alessio Soggiu, Viviana Greco, Luigi Bonizzi, Paola Roncada, Paola G Brambilla
BACKGROUND: Myxomatous mitral valve disease (MVD) is the most common acquired heart disease in dogs, and the Cavalier King Charles Spaniel (CKCS) is the most studied breed because of the high prevalence, early onset and hereditary component evidenced in the breed. MVD has different severity levels, and there are many practical limitations in identifying its asymptomatic stages. Proteomic techniques are valuable for studying the proteins and peptides involved in cardiovascular diseases, including the period prior to the clinical onset of the disease...
February 7, 2017: BMC Veterinary Research
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