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Yue Song, Xing Xin, Xingyue Zhai, Zhijun Xia, Keng Shen
BACKGROUND: Induction of cell apoptosis and regulation of cell cycle are very attractive for treatments of tumors including ovarian carcinoma. Flavopiridol is a potent small molecular cyclin-dependent kinase(cdk) inhibitor, but its antitumor efficacy is not satisfied yet. Caspase-3 play a major role in the transduction of apoptotic signals and the execution of apoptosis in mammalian cells. We have successfully constructed the recombinant adenovirues AdHTVP2G5-rev-casp3 containing autocatalytic caspase-3 (rev-caspase-3) driven by amplified hTERT promoter system (TSTA-hTERTp)...
2014: Journal of Ovarian Research
Feng Zhao, Qinghua Zhou, Yang Qin, Zhiling Sun, Zefang Sun
BACKGROUND: To construct the recombinant adenovirus of mutant k-ras by using the method of homogenous recombination in bacteria. METHODS: Mutant k-ras gene was liberated from the vector of pcDNA3-k-ras 12(Val) via KpnI+XhoI digestion, and subcloned into shuttle vector of pAdTrack-CMV, forming transfer vector of pAdTrack CMV/k-ras 12(Val). Then it was linearized with PmeI and cotransformed into BJ5183 cells with adenovirual geonomis plasmid of pAdEasy-1. The DNA of identified recombinant plasmid was digested with PacI and transfected to 293 cells to package adenovirus...
February 20, 2002: Zhongguo Fei Ai za Zhi, Chinese Journal of Lung Cancer
Si-Xiu Li, Guo-Cheng Zhang, Dong-Liang Xu, Xiao-Jing Nie, Xiao-Qing Li, Zhi-Hua Wang, Xue-Hong Zhang
AIM: To prepare, identify and apply anti-human adenoviru(HAdv)neutralization monoclonal antibody(mAb). METHODS: BALB/c mice were immunized with live human adenovirus type3(HAdv-3) strain intranarially. Sp2/0 cells were fused with the spleen cells harvested from BALB/c mice. The chromosomal amounts of the hybridoma cells were analyzed by colchicine. A commercially available mouse mAb isotyping kit was used to identify the isotype of this mAb. Clones secreting specific monoclonal antibody were screened by indirect enzyme linked immunosorbent assay (ELISA), Western blot and indirect immunofluorescent assay...
December 2009: Xi Bao Yu Fen Zi Mian Yi Xue za Zhi, Chinese Journal of Cellular and Molecular Immunology
Jin Soo Park, Kensei Nagata
Some evidence indicates that intervertebral disc degeneration is associated with decreasing of matrix synthesis from disc cell. BMP-2 increased mRNA of aggrecan and Type II collagen. It also enhanced disc matrix (proteoglycan) production. Adenovirually mediated LMP-1 overexpression in intervertebral disc cells increased disc cell proteoglycan production through BMP-2 and BMP-7 mediated process.
July 2004: Clinical Calcium
Guodong Gao, Tao Huang, Siyuan Chen
OBJECTIVE: To investigate in vitro and in vivo bystander effect, including distance bystander effect of adenovirus-mediated transfer of the herpes simplex virus thymidine kinase gene (HSV-tk). METHODS: In vitro, mixed tk + BEL-7402 cells and tk-BEL-7402 cells in diverse proportions and ganciclovir (GCV) was given, then tested the survival ratio of cells by MTT. In vivo, 5 x 10(6) and 5 x 10(7) tk + BEL-7402 cells were injected into the tumors in nude mice following GCV...
April 2002: Zhonghua Wai Ke za Zhi [Chinese Journal of Surgery]
J Y Dong, D Wang, F W Van Ginkel, D W Pascual, R A Frizzell
Adenovirus-based vectors are promising candidates for genetic therapy of cystic fibrosis (CF). Because adenoviruses naturally infect airway cells, they grow to very high titers, and the transgenes carried by the adenoviruses are expressed at high levels. In addition, adenoviruses are relatively safe because the disease caused by the wild-type virus is self-limiting. One disadvantage of adenovirual vectors is that the transgene expression would be transient because adenoviruses do not integrate their DNA into the genome of the host cells...
February 10, 1996: Human Gene Therapy
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