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Yuan Zhang, Yifan Ping, Ruyu Zhou, Juan Wang, Guangdong Zhang
This study aims to explore the water quality of dental unit waterlines (DUWLs) and the diversity of microbial communities in DUWLs. Water samples from 33 dental chair units (DCUs) were collected, diluted and then spread on sterilized R2A plate for incubation. Subsequently, the microbial colony-forming units per milliliter (CFU/ml) were recorded by an automatic colony analyzer. Total DNA extracted from the rest of the samples was tested on the Illumina MiSeq PE300 platform. T-test and Kruskal-Wallis rank test were adopted for statistical analysis...
October 5, 2017: Journal of Infection and Public Health
Tingting Li, Man Lu, Jun Song, Ping Wu, Xueqing Cheng, Zhenqi Zhang
OBJECTIVE: The purpose of this retrospective study is to evaluate the diagnostic value of contrast enhanced sonography plus gastric distention sonography, the Double Contrast-enhanced Ultrasound (DCUS) in gastric lesions. METHODS: 107 cases with pathology confirmed gastric lesions were retrospectively reviewed, DCUS and oral contrast agent ultrasound (US) were performed in all cases prior to operation. Perfusion parameters including arrival time (AT), peak intensity (PI), time to peak (TTP), and area under the curve (AUC) of the lesion and surrounding normal tissue were analyzed...
2017: PloS One
Dan Wang, Weizhong Chen, Shanqing Huang, Yafeng He, Xichun Liu, Qingyuan Hu, Tianbiao Wei, Hong Sang, Jianhua Gan, Hao Chen
Pseudomonas aeruginosa (P. aeruginosa) is a major opportunistic human pathogen, causing serious nosocomial infections among immunocompromised patients by multi-determinant virulence and high antibiotic resistance. The CzcR-CzcS signal transduction system in P. aeruginosa is primarily involved in metal detoxification and antibiotic resistance through co-regulating cross-resistance between Zn(II) and carbapenem antibiotics. Although the intracellular regulatory pathway is well-established, the mechanism by which extracellular sensor domain of histidine kinase (HK) CzcS responds to Zn(II) stimulus to trigger downstream signal transduction remains unclear...
July 2017: PLoS Pathogens
E Sedlata Juraskova, H Sedlackova, J Janska, O Holy, I Lalova, I Matouskova
OBJECTIVE: To determine the current presence of Legionella spp. in the output water of dental unit waterlines (DUWLs) and examine its mitigation by disinfection at the Institute of Dentistry and Oral Sciences, Faculty of Medicine and Dentistry, Palacky University Olomouc and University Hospital Olomouc. MATERIAL AND METHODS: The first stage of our survey involved collecting samples of DUWL output water from 50 dental chair units (DCUs), and 2 samples of the incoming potable water...
2017: Bratislavské Lekárske Listy
Thomas J Finn, Sonal Shewaramani, Sinead C Leahy, Peter H Janssen, Christina D Moon
BACKGROUND: Many bacteria are facultative anaerobes, and can proliferate in both anoxic and oxic environments. Under anaerobic conditions, fermentation is the primary means of energy generation in contrast to respiration. Furthermore, the rates and spectra of spontaneous mutations that arise during anaerobic growth differ to those under aerobic growth. A long-term selection experiment was undertaken to investigate the genetic changes that underpin how the facultative anaerobe, Escherichia coli, adapts to anaerobic environments...
2017: PeerJ
Ashish Vashist, Swati Parhar, Ramandeep Singh Gambhir, Ramandeep Kaur Sohi, Puneet Singh Talwar
BACKGROUND: Health system should be organized to meet the needs of entire population of the nation. This means that the state has the direct responsibility for the health of its population and improving the quality of life through research, education, and provision of health services. The present study was conducted to evaluate the government oral health care delivery system in Haryana, India. MATERIALS AND METHODS: The present cross-sectional study was conducted among 135 dental care units (DCUs) of various primary health centers (PHCs), community health centers (CHCs), and general hospitals (GHs) existing in the state by employing a cluster random sampling technique...
July 2016: Journal of Family Medicine and Primary Care
S-F Zhou, J-B Yin, H Yang, J Zhong, P An
OBJECTIVE: To explore the imaging characteristics and clinical application value of gastric filling ultrasound combined with an intravenous contrast agent (double contrast ultrasonography, DCUS) in the diagnosis of advanced gastric carcinoma. PATIENTS AND METHODS: Totally 48 patients with advanced gastric carcinoma were confirmed by endoscopic biopsy and then administrated with DCUS and enhanced magnetic resonance examination before treatment. Retrospective analysis was conducted based on the characteristics of their ultrasonic images, and the results of diagnosis were compared and analyzed between the DCUS technology and the enhanced magnetic resonance examination...
July 2016: European Review for Medical and Pharmacological Sciences
Gottfried Unden, Alexander Strecker, Alexandra Kleefeld, Ok Bin Kim
C4-dicarboxylates and the C4-dicarboxylic amino acid l-aspartate support aerobic and anaerobic growth of Escherichia coli and related bacteria. In aerobic growth, succinate, fumarate, D- and L-malate, L-aspartate, and L-tartrate are metabolized by the citric acid cycle and associated reactions. Because of the interruption of the citric acid cycle under anaerobic conditions, anaerobic metabolism of C4-dicarboxylates depends on fumarate reduction to succinate (fumarate respiration). In some related bacteria (e...
June 2016: EcoSal Plus
Sebastian Wörner, Alexander Strecker, Christian Monzel, Matthias Zeltner, Julian Witan, Andrea Ebert-Jung, Gottfried Unden
The sensor kinase DcuS of Escherichia coli co-operates under aerobic conditions with the C4 -dicarboxylate transporter DctA to form the DctA/DcuS sensor complex. Under anaerobic conditions C4 -dicarboxylate transport in fumarate respiration is catalyzed by C4 -dicarboxylate/fumarate antiporter DcuB. (i) DcuB interacted with DcuS as demonstrated by a bacterial two-hybrid system (BACTH) and by co-chromatography of the solubilized membrane-proteins (mHPINE assay). (ii) In the DcuB/DcuS complex only DcuS served as the sensor since mutations in the substrate site of DcuS changed substrate specificity of sensing, and substrates maleate or 3-nitropropionate induced DcuS response without affecting the fumarate site of DcuB...
December 2016: Environmental Microbiology
G Unden, S Wörner, C Monzel
Many membrane-bound sensor kinases require accessory proteins for function. The review describes functional control of membrane-bound sensors by transporters. The C4-dicarboxylate sensor kinase DcuS requires the aerobic or anaerobic C4-dicarboxylate transporters DctA or DcuB, respectively, for function and forms DctA/DcuS or DcuB/DcuS sensor complexes. Free DcuS is in the permanent (ligand independent) ON state. The DctA/DcuS and DcuB/DcuS complexes, on the other hand, control expression in response to C4-dicarboxylates...
2016: Advances in Microbial Physiology
Xue-Yue Ji, Chun-Nan Fei, Ying Zhang, Wei Zhang, Jun Liu, Jie Dong
INTRODUCTION: Dental unit waterline (DUWL) output water is delivered through instruments of a dental chair unit (DCU) to irrigate and cool teeth. However, these waterlines can be heavily contaminated with bacteria. AIM: The purpose of the present study was to assess retraction and investigate the contamination level and prevalence of bacteria in DUWL output water. METHODS: Fifty-eight DCUs were randomly selected from 30 hospitals in 10 districts of Tianjin, one of the four special municipalities of China...
August 2016: International Dental Journal
Larry Reitzer
Glutamate, aspartate, asparagine, L-alanine, and D-alanine are derived from intermediates of central metabolism, mostly the citric acid cycle, in one or two steps. While the pathways are short, the importance and complexity of the functions of these amino acids befit their proximity to central metabolism. Inorganic nitrogen (ammonia) is assimilated into glutamate, which is the major intracellular nitrogen donor. Glutamate is a precursor for arginine, glutamine, proline, and the polyamines. Glutamate degradation is also important for survival in acidic environments, and changes in glutamate concentration accompany changes in osmolarity...
December 2004: EcoSal Plus
Ramón De las Peñas, Santiago Ponce, Fernando Henao, Carlos Camps Herrero, Enric Carcereny, Yolanda Escobar Álvarez, César A Rodríguez, Juan Antonio Virizuela, Rafael López López
Hyponatremia (Na ˂ 135 mmol/l) is the most frequent electrolyte disorder in clinical practice, and the syndrome of inappropriate antidiuretic hormone secretion (SIADH) is the commonest cause of hyponatremia in cancer patients. Correcting hyponatremia in these patients can reduce morbidity and mortality, increase the response to anti-cancer agents, and help reduce hospital length of stay and costs. Tolvaptan is an oral medication used to treat SIADH-related hyponatremia patients that needs to be initiated at hospital so patients can have their serum sodium monitored...
January 2016: Supportive Care in Cancer: Official Journal of the Multinational Association of Supportive Care in Cancer
Sabrina Graf, Constanze Broll, Juliane Wissig, Alexander Strecker, Maria Parowatkin, Gottfried Unden
The thermophilic Geobacillus thermodenitrificans and Geobacillus kaustophilus are able to use citrate or C4-dicarboxylates like fumarate or succinate as the substrates for growth. The genomes of the sequenced Geobacillus strains (nine strains) each encoded a two-component system of the CitA family. The sensor kinase of G. thermodenitrificans (termed CitAGt) was able to replace CitA of Escherichia coli (CitAEc) in a heterologous complementation assay restoring expression of the CitAEc-dependent citC-lacZ reporter gene and anaerobic growth on citrate...
January 2016: Microbiology
Fatima Abdouchakour, Chloé Dupont, Delphine Grau, Fabien Aujoulat, Patricia Mournetas, Hélène Marchandin, Sylvie Parer, Philippe Gibert, Jean Valcarcel, Estelle Jumas-Bilak
Dental care unit waterlines (DCUWs) consist of complex networks of thin tubes that facilitate the formation of microbial biofilms. Due to the predilection toward a wet environment, strong adhesion, biofilm formation, and resistance to biocides, Pseudomonas aeruginosa, a major human opportunistic pathogen, is adapted to DCUW colonization. Other nonfermentative Gram-negative bacilli, such as members of the genus Achromobacter, are emerging pathogens found in water networks. We reported the 6.5-year dynamics of bacterial contamination of waterlines in a dental health care center with 61 dental care units (DCUs) connected to the same water supply system...
November 2015: Applied and Environmental Microbiology
Christian Monzel, Gottfried Unden
The C4-dicarboxylate sensor kinase DcuS is membrane integral because of the transmembrane (TM) helices TM1 and TM2. Fumarate-induced movement of the helices was probed in vivo by Cys accessibility scanning at the membrane-water interfaces after activation of DcuS by fumarate at the periplasmic binding site. TM1 was inserted with amino acid residues 21-41 in the membrane in both the fumarate-activated (ON) and inactive (OFF) states. In contrast, TM2 was inserted with residues 181-201 in the OFF state and residues 185-205 in the ON state...
September 1, 2015: Proceedings of the National Academy of Sciences of the United States of America
M A Boyle, M J O'Donnell, R J Russell, N Galvin, J Swan, D C Coleman
OBJECTIVES: Decontaminating dental chair unit (DCU) suction systems in a convenient, safe and effective manner is problematic. This study aimed to identify and quantify the extent of the problems using 25 DCUs, methodically eliminate these problems and develop an efficient approach for reliable, effective, automated disinfection. METHODS: DCU suction system residual contamination by environmental and human-derived bacteria was evaluated by microbiological culture following standard aspiration disinfection with a quaternary ammonium disinfectant or alternatively, a novel flooding approach to disinfection...
October 2015: Journal of Dentistry
W Ben Mortenson, Anne Marie Bishop
Specialized dementia care units (DCUs) house individuals whose behaviors cannot be managed in other settings. To ensure environmental fit, admission/discharge criteria are recommended for DCUs; however, there is no consensus about what criteria should be used. This study aimed to describe, in a random sample of DCUs, the current admission criteria, current/recommended discharge criteria, and services to support discharge. Usable surveys were returned by 23 of 30 facilities. Residents were most frequently admitted because they had a diagnosis of dementia and exhibited cognitive/behavioral problems...
March 2016: Journal of Applied Gerontology: the Official Journal of the Southern Gerontological Society
José A Reyes-Darias, Yiling Yang, Victor Sourjik, Tino Krell
The PctA and PctB chemoreceptors of Pseudomonas aeruginosa mediate chemotaxis toward amino acids. A general feature of signal transduction processes is that a signal input is converted into an output. We have generated chimeras combining the Tar signaling domain with either the PctA or PctB ligand binding domain (LBD). Escherichia coli harboring either PctA-Tar or PctB-Tar mediated chemotaxis toward amino acids. The responses of both chimeras were determined using fluorescence resonance energy transfer, and the derived EC50 values are a measure of output...
May 2015: Molecular Microbiology
Patrick D Scheu, Philipp A Steinmetz, Felix Dempwolff, Peter L Graumann, Gottfried Unden
The C4-dicarboxylate responsive sensor kinase DcuS of the DcuS/DcuR two-component system of E. coli is membrane-bound and reveals a polar localization. DcuS uses the C4-dicarboxylate transporter DctA as a co-regulator forming DctA/DcuS sensor units. Here it is shown by fluorescence microscopy with fusion proteins that DcuS has a dynamic and preferential polar localization, even at very low expression levels. Single assemblies of DcuS had high mobility in fast time lapse acquisitions, and fast recovery in FRAP experiments, excluding polar accumulation due to aggregation...
2014: PloS One
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