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https://www.readbyqxmd.com/read/27910960/unc-5-homolog-b-unc5b-is-one-of-the-key-downstream-targets-of-n-%C3%AE-acetyltransferase-10-naa10
#1
Huiyu Xu, Yong Han, Bing Liu, Rong Li
N-α-acetyltransferase 10 (Naa10) displays alpha (N-terminal) acetyltransferase activity. It functions as a major modulator of cell growth and differentiation. Until now, a few downstream targets were found, but no studies have concerned about which gene is the early event of Naa10 downstream target. As we know, the earlier events may play more significant role in Naa10 pathway. Through construction of Naa10 stably knocked down H1299 cell line, we discovered cell morphological changes induced by Naa10. Moreover, potential function of Naa10 in cell morphogenesis was also indicated using cDNA microarray analysis of the Naa10 stably knock-down cell line...
December 2, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27901020/structural-decoding-of-netrin-4-reveals-a-regulatory-function-towards-mature-basement-membranes
#2
Raphael Reuten, Trushar R Patel, Matthew McDougall, Nicolas Rama, Denise Nikodemus, Benjamin Gibert, Jean-Guy Delcros, Carina Prein, Markus Meier, Stéphanie Metzger, Zhigang Zhou, Jennifer Kaltenberg, Karen K McKee, Tobias Bald, Thomas Tüting, Paola Zigrino, Valentin Djonov, Wilhelm Bloch, Hauke Clausen-Schaumann, Ernst Poschl, Peter D Yurchenco, Martin Ehrbar, Patrick Mehlen, Jörg Stetefeld, Manuel Koch
Netrins, a family of laminin-related molecules, have been proposed to act as guidance cues either during nervous system development or the establishment of the vascular system. This was clearly demonstrated for netrin-1 via its interaction with the receptors DCC and UNC5s. However, mainly based on shared homologies with netrin-1, netrin-4 was also proposed to play a role in neuronal outgrowth and developmental/pathological angiogenesis via interactions with netrin-1 receptors. Here, we present the high-resolution structure of netrin-4, which shows unique features in comparison with netrin-1, and show that it does not bind directly to any of the known netrin-1 receptors...
November 30, 2016: Nature Communications
https://www.readbyqxmd.com/read/27890825/the-expression-of-chemorepulsive-guidance-receptors-and-the-regenerative-abilities-of-spinal-projecting-neurons-after-spinal-cord-injury
#3
Jie Chen, Cindy Laramore, Michael I Shifman
Spinal cord injury (SCI) in mammals leads to permanent loss of function because axons do not regenerate in the central nervous system (CNS). To date, treatments based on neutralizing inhibitory environmental cues, such as the myelin-associated growth inhibitors and chondroitin sulfate proteoglycans, or on adding neurotrophic factors, have had limited success in enhancing regeneration. Published studies suggested that multiple axon guidance cues (repulsive guidance molecule (RGM) family, semaphorins, ephrins, and netrins) persist in adult animals, and that their expression is upregulated after CNS injury...
November 24, 2016: Neuroscience
https://www.readbyqxmd.com/read/27881869/netrin-1-induced-stem-cell-bioactivity-contributes-to-the-regeneration-of-injured-tissues-via-the-lipid-raft-dependent-integrin-%C3%AE-6%C3%AE-4-signaling-pathway
#4
Soo Sang Lee, Sei-Jung Lee, Sang Hun Lee, Jung Min Ryu, Hyeon Su Lim, Jun Sung Kim, Eun Ju Song, Young Hyun Jung, Hyun Jik Lee, Chung Hun Kim, Ho Jae Han
Netrin-1 (Ntn-1) is a multifunctional neuronal signaling molecule; however, its physiological significance, which improves the tissue-regeneration capacity of stem cells, has not been characterized. In the present study, we investigate the mechanism by which Ntn-1 promotes the proliferation of hUCB-MSCs with regard to the regeneration of injured tissues. We found that Ntn-1 induces the proliferation of hUCB-MSCs mainly via Inα6β4 coupled with c-Src. Ntn-1 induced the recruitment of NADPH oxidases and Rac1 into membrane lipid rafts to facilitate ROS production...
November 24, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27872885/data-comparing-the-kinetics-of-procollagen-type-i-processing-by-bone-morphogenetic-protein-1-bmp-1-with-and-without-procollagen-c-proteinase-enhancer-1-pcpe-1
#5
Laura Moschcovich, Efrat Kessler
This article provides kinetic constants for C-terminal processing of procollagen type I by bone morphogenetic protein 1 (BMP-1; the major procollagen C-proteinase), a reaction stimulated by the connective tissue glycoprotein procollagen C-proteinase enhancer 1 (PCPE-1). Reported are Km , Vmax , Kcat and Kcat /Km (catalytic coefficient) values for BMP-1 alone, BMP-1 with intact PCPE-1, BMP-1 with the CUB (Complement C1r/C1s, Uegf, BMP-1) domains fragment of PCPE-1 as well as its NTR (netrin-like) domain.
December 2016: Data in Brief
https://www.readbyqxmd.com/read/27863093/potential-effect-of-mechano-growth-factor-e-domain-peptide-on-axonal-guidance-growth-in-primary-cultured-cortical-neurons-of-rats
#6
Meili Liu, Xufeng Niu, Gang Zhou, Zhengtai Jia, Ping Li, Yubo Fan
Establishing appropriate synaptic connections and plasticity is a critical need in neuronal regeneration and development. Mechano growth factor (MGF) and its C-terminal E-domain peptide with 24 amino acids, MGF-Ct24E, are potential neuroprotective agents. Our preliminary study indicates that Netrin-1 can guide axonal growth and its expression is sensitive to MGF, but how MGF regulates the expression of Netrin-1 and its receptor DCC is still unclear. Here, we investigate the effect of MGF-Ct24E on the expression of Netrin-1 and DCC in primary cultured cortical neurons in vitro and the adult rat brain in vivo...
November 15, 2016: Journal of Tissue Engineering and Regenerative Medicine
https://www.readbyqxmd.com/read/27856613/dorsal-horn-interneuron-derived-netrin-4-contributes-to-spinal-sensitization-in-chronic-pain-via-unc5b
#7
Yasufumi Hayano, Keiko Takasu, Yoshihisa Koyama, Moe Yamada, Koichi Ogawa, Kazuhisa Minami, Toshiyuki Asaki, Kazuhiro Kitada, Satoshi Kuwabara, Toshihide Yamashita
Because of the incomplete understanding of the molecular mechanisms that underlie chronic pain, the currently available treatments for this type of pain remain inefficient. In this study, we show that Netrin-4, a member of the axon guidance molecule family, was expressed in dorsal horn inner lamina II excitatory interneurons in the rat spinal cord. A similar expression pattern for Netrin-4 was also observed in human spinal cord. Behavioral analysis revealed that tactile and heat hyperalgesia after peripheral nerve injury or inflammation were abolished in Netrin-4-mutant rats...
November 17, 2016: Journal of Experimental Medicine
https://www.readbyqxmd.com/read/27846823/overexpression-of-unc5b-in-bladder-cancer-cells-inhibits-proliferation-and-reduces-the-volume-of-transplantation-tumors-in-nude-mice
#8
Chuize Kong, Bo Zhan, Chiyuan Piao, Zhe Zhang, Yuyan Zhu, Qingchang Li
BACKGROUND: The netrin-1 receptor UNC5B plays vital roles in angiogenesis, inflammation, embryonic development and carcinogenesis. However, the functional significance of UNC5B overexpression in bladder cancer remains unclear. In this study, we investigated the role of UNC5B in bladder cancer in vitro and in vivo. METHODS: Stable transfection of the human bladder cancer cell line 5637 with UNC5B (5637-U) was confirmed by real-time RT-PCR, western blot and immunofluorescence assays...
November 15, 2016: BMC Cancer
https://www.readbyqxmd.com/read/27815019/expression-of-netrin-1-by-hypoxia-contributes-to-the-invasion-and-migration-of-prostate-carcinoma-cells-by-regulating-yap-activity
#9
Haiwen Chen, Qi Chen, Qidong Luo
Hypoxia is a hallmark of solid tumor growth microenvironment and appropriates the major contributor for the failure and poor prognosis of clinical tumor treatment, including prostate cancer (PCa). Ectopic expression of netrin-1 is reportedly associated with the progression of several carcinomas. Here, we aimed to investigate the role of netrin-1 in hypoxic metastasis potential of prostate carcinoma. Here, hypoxia induced the up-regulation of netrin-1 mRNA and protein expression in prostate cancer cell lines PC3 and DU145...
November 1, 2016: Experimental Cell Research
https://www.readbyqxmd.com/read/27793362/netrin-1-a-regulator-of-cancer-cell-motility
#10
Irene Ylivinkka, Jorma Keski-Oja, Marko Hyytiäinen
Netrins form a family of secreted and membrane-associated proteins, netrin-1 being the prototype and most investigated member of the family. The major physiological functions of netrin-1 lie in the regulation of axonal development as well as morphogenesis of different branched organs, by promoting the polarity of migratory/invasive front of the cell. On the other hand, netrin-1 acts as a factor preventing cell apoptosis. These events are mediated via a range of different receptors, including UNC5 and DCC-families...
October 19, 2016: European Journal of Cell Biology
https://www.readbyqxmd.com/read/27787862/photolithography-based-substrate-microfabrication-for-patterning-semaphorin-3a-to-study-neuronal-development
#11
Maya Shelly, Seong-Ii Lee, Giulia Suarato, Yizhi Meng, Sophie Pautot
Protein micropatterning techniques, including microfluidic devices and protein micro-contact printing, enable the generation of highly controllable substrates for spatial manipulation of intracellular and extracellular signaling determinants to examine the development of cultured dissociated neurons in vitro. In particular, culture substrates coated with proteins of interest in defined stripes, including cell adhesion molecules and secreted proteins, have been successfully used to study neuronal polarization, a process in which the neuron establishes axon and dendrite identities, a critical architecture for the input/output functions of the neuron...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/27773352/dcc-confers-susceptibility-to-depression-like-behaviors-in-humans-and-mice-and-is-regulated-by-mir-218
#12
Angélica Torres-Berrío, Juan Pablo Lopez, Rosemary C Bagot, Dominique Nouel, Gregory Dal Bo, Santiago Cuesta, Lei Zhu, Colleen Manitt, Conrad Eng, Helen M Cooper, Kai-Florian Storch, Gustavo Turecki, Eric J Nestler, Cecilia Flores
BACKGROUD: Variations in the expression of the Netrin-1 guidance cue receptor DCC (deleted in colorectal cancer) appear to confer resilience or susceptibility to psychopathologies involving prefrontal cortex (PFC) dysfunction. METHODS: With the use of postmortem brain tissue, mouse models of defeat stress, and in vitro analysis, we assessed microRNA (miRNA) regulation of DCC and whether changes in DCC levels in the PFC lead to vulnerability to depression-like behaviors...
August 18, 2016: Biological Psychiatry
https://www.readbyqxmd.com/read/27770832/contralateral-migration-of-oculomotor-neurons-is-regulated-by-slit-robo-signaling
#13
Brielle Bjorke, Farnaz Shoja-Taheri, Minkyung Kim, G Eric Robinson, Tatiana Fontelonga, Kyung-Tai Kim, Mi-Ryoung Song, Grant S Mastick
BACKGROUND: Oculomotor neurons develop initially like typical motor neurons, projecting axons out of the ventral midbrain to their ipsilateral targets, the extraocular muscles. However, in all vertebrates, after the oculomotor nerve (nIII) has reached the extraocular muscle primordia, the cell bodies that innervate the superior rectus migrate to join the contralateral nucleus. This motor neuron migration represents a unique strategy to form a contralateral motor projection. Whether migration is guided by diffusible cues remains unknown...
October 22, 2016: Neural Development
https://www.readbyqxmd.com/read/27759917/implication-of-lrrc4c-and-dpp6-in-neurodevelopmental-disorders
#14
Gilles Maussion, Cristiana Cruceanu, Jill A Rosenfeld, Scott C Bell, Fabrice Jollant, Jin Szatkiewicz, Ryan L Collins, Carrie Hanscom, Ilaria Kolobova, Nicolas Menjot de Champfleur, Ian Blumenthal, Colby Chiang, Vanessa Ota, Christina Hultman, Colm O'Dushlaine, Steve McCarroll, Martin Alda, Sebastien Jacquemont, Zehra Ordulu, Christian R Marshall, Melissa T Carter, Lisa G Shaffer, Pamela Sklar, Santhosh Girirajan, Cynthia C Morton, James F Gusella, Gustavo Turecki, Dimitri J Stavropoulos, Patrick F Sullivan, Stephen W Scherer, Michael E Talkowski, Carl Ernst
We performed whole-genome sequencing on an individual from a family with variable psychiatric phenotypes that had a sensory processing disorder, apraxia, and autism. The proband harbored a maternally inherited balanced translocation (46,XY,t(11;14)(p12;p12)mat) that disrupted LRRC4C, a member of the highly specialized netrin G family of axon guidance molecules. The proband also inherited a paternally derived chromosomal inversion that disrupted DPP6, a potassium channel interacting protein. Copy Number (CN) analysis in 14,077 cases with neurodevelopmental disorders and 8,960 control subjects revealed that 60% of cases with exonic deletions in LRRC4C had a second clinically recognizable syndrome associated with variable clinical phenotypes, including 16p11...
October 19, 2016: American Journal of Medical Genetics. Part A
https://www.readbyqxmd.com/read/27746720/hyperexpressed-netrin-1-promoted-neural-stem-cells-migration-in-mice-after-focal-cerebral-ischemia
#15
Haiyan Lu, Xiaoyan Song, Feng Wang, Guodong Wang, Yuncheng Wu, Qiaoshu Wang, Yongting Wang, Guo-Yuan Yang, Zhijun Zhang
Endogenous Netrin-1 (NT-1) protein was significantly increased after cerebral ischemia, which may participate in the repair after transient cerebral ischemic injury. In this work, we explored whether NT-1 can be steadily overexpressed by adeno-associated virus (AAV) and the exogenous NT-1 can promote neural stem cells migration from the subventricular zone (SVZ) region after cerebral ischemia. Adult CD-1 mice were injected stereotacticly with AAV carrying NT-1 gene (AAV-NT-1). Mice underwent 60 min of middle cerebral artery (MCA) occlusion 1 week after injection...
2016: Frontiers in Cellular Neuroscience
https://www.readbyqxmd.com/read/27743477/frazzled-promotes-growth-cone-attachment-at-the-source-of-a-netrin-gradient-in-the-drosophila-visual-system
#16
Orkun Akin, S Lawrence Zipursky
Axon guidance is proposed to act through a combination of long- and short-range attractive and repulsive cues. The ligand-receptor pair, Netrin (Net) and Frazzled (Fra) (DCC, Deleted in Colorectal Cancer, in vertebrates), is recognized as the prototypical effector of chemoattraction, with roles in both long- and short-range guidance. In the Drosophila visual system, R8 photoreceptor growth cones were shown to require Net-Fra to reach their target, the peak of a Net gradient. Using live imaging, we show, however, that R8 growth cones reach and recognize their target without Net, Fra, or Trim9, a conserved binding partner of Fra, but do not remain attached to it...
October 15, 2016: ELife
https://www.readbyqxmd.com/read/27740455/the-glide-gcm-fate-determinant-controls-initiation-of-collective-cell-migration-by-regulating-frazzled
#17
Tripti Gupta, Arun Kumar, Pierre B Cattenoz, K VijayRaghavan, Angela Giangrande
Collective migration is a complex process that contributes to build precise tissue and organ architecture. Several molecules implicated in cell interactions also control collective migration, but their precise role and the finely tuned expression that orchestrates this complex developmental process are poorly understood. Here, we show that the timely and threshold expression of the Netrin receptor Frazzled triggers the initiation of glia migration in the developing Drosophila wing. Frazzled expression is induced by the transcription factor Glide/Gcm in a dose-dependent manner...
October 14, 2016: ELife
https://www.readbyqxmd.com/read/27733818/expression-patterns-of-extracellular-matrix-proteins-during-posterior-commissure-development
#18
Karen Stanic, Natalia Saldivia, Benjamín Förstera, Marcela Torrejón, Hernán Montecinos, Teresa Caprile
Extracellular matrix (ECM) molecules are pivotal for central nervous system (CNS) development, facilitating cell migration, axonal growth, myelination, dendritic spine formation, and synaptic plasticity, among other processes. During axon guidance, the ECM not only acts as a permissive or non-permissive substrate for navigating axons, but also modulates the effects of classical guidance cues, such as netrin or Eph/ephrin family members. Despite being highly important, little is known about the expression of ECM molecules during CNS development...
2016: Frontiers in Neuroanatomy
https://www.readbyqxmd.com/read/27727089/netrin-1-is-associated-with-macrophage-infiltration-and-polarization-in-human-epicardial-adipose-tissue-in-coronary-artery-disease
#19
Kadri Murat Gurses, Fusun Ozmen, Duygu Kocyigit, Nilgun Yersal, Elif Bilgic, Erkan Kaya, Cagla Zubeyde Kopru, Tolga Soyal, Suat Doganci, Lale Tokgozoglu, Petek Korkusuz
BACKGROUND: Inflammatory activity originating from the epicardial adipose tissue (EAT) may have a role in coronary artery disease (CAD) pathogenesis. The relationship between macrophage infiltration, polarization in the EAT, and netrin-1 gene expression was investigated. METHODS: Macrophage infiltration and polarization were examined by immunohistochemical methods and expression levels of netrin-1, Unc5b, and cytokines related with M1-macrophage subtype (IL-12 and IL-18) were determined by quantitative polymerase chain reaction in subcutaneous and epicardial adipose tissue obtained from patients undergoing coronary artery bypass grafting and non-coronary cardiac surgery...
October 7, 2016: Journal of Cardiology
https://www.readbyqxmd.com/read/27681594/bone-protective-functions-of-netrin-1-protein
#20
Kenta Maruyama, Takahiko Kawasaki, Masahide Hamaguchi, Motomu Hashimoto, Moritoshi Furu, Hiromu Ito, Takao Fujii, Naoki Takemura, Thangaraj Karuppuchamy, Takeshi Kondo, Takumi Kawasaki, Masahiro Fukasaka, Takuma Misawa, Tatsuya Saitoh, Yutaka Suzuki, Mikaël M Martino, Yutaro Kumagai, Shizuo Akira
Netrin 1 was initially identified as an axon guidance factor, and recent studies indicate that it inhibits chemokine-directed monocyte migration. Despite its importance as a neuroimmune guidance cue, the role of netrin 1 in osteoclasts is largely unknown. Here we detected high netrin 1 levels in the synovial fluid of rheumatoid arthritis patients. Netrin 1 is potently expressed in osteoblasts and synovial fibroblasts, and IL-17 robustly enhances netrin 1 expression in these cells. The binding of netrin 1 to its receptor UNC5b on osteoclasts resulted in activation of SHP1, which inhibited VAV3 phosphorylation and RAC1 activation...
November 11, 2016: Journal of Biological Chemistry
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