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https://www.readbyqxmd.com/read/28338079/dissecting-mirna-gene-repression-on-single-cell-level-with-an-advanced-fluorescent-reporter-system
#1
Nicolas Lemus-Diaz, Kai O Böker, Ignacio Rodriguez-Polo, Michael Mitter, Jasmin Preis, Maximilian Arlt, Jens Gruber
Despite major advances on miRNA profiling and target predictions, functional readouts for endogenous miRNAs are limited and frequently lead to contradicting conclusions. Numerous approaches including functional high-throughput and miRISC complex evaluations suggest that the functional miRNAome differs from the predictions based on quantitative sRNA profiling. To resolve the apparent contradiction of expression versus function, we generated and applied a fluorescence reporter gene assay enabling single cell analysis...
March 24, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28316235/evaluation-of-nanobody-conjugates-and-protein-fusions-as-bioanalytical-reagents
#2
Virginia J Bruce, Brian R McNaughton
Enzyme-linked immunosorbent assay (ELISA), flow cytometry, and Western blot are common bioanalytical techniques. Successful execution traditionally requires the use of one or more commercially available antibody-small-molecule dye, or antibody-reporter protein conjugates that recognize relatively short peptide tags (<15 amino acids). However, the size of antibodies, and their molecular complexity (by virtue of post-translational disulfide formation and glycosylation) typically requires either expression in mammalian cells or purification from immunized mammals...
March 20, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/28292715/footwear-characteristics-are-related-to-running-mechanics-in-runners-with-patellofemoral-pain
#3
Jean-Francois Esculier, Blaise Dubois, Laurent J Bouyer, Bradford J McFadyen, Jean-Sébastien Roy
Running footwear is known to influence step rate, foot inclination at foot strike, average vertical loading rate (VLR) and peak patellofemoral joint (PFJ) force. However, the association between the level of minimalism of running shoes and running mechanics, especially with regards to these relevant variables for runners with patellofemoral pain (PFP), has yet to be investigated. The objective of this study was to explore the relationship between the level of minimalism of running shoes and habitual running kinematics and kinetics in runners with PFP...
March 6, 2017: Gait & Posture
https://www.readbyqxmd.com/read/28265506/immediate-effects-of-eva-midsole-resilience-and-upper-shoe-structure-on-running-biomechanics-a-machine-learning-approach
#4
Andrea N Onodera, Wilson P Gavião Neto, Maria Isabel Roveri, Wagner R Oliveira, Isabel Cn Sacco
BACKGROUND: Resilience of midsole material and the upper structure of the shoe are conceptual characteristics that can interfere in running biomechanics patterns. Artificial intelligence techniques can capture features from the entire waveform, adding new perspective for biomechanical analysis. This study tested the influence of shoe midsole resilience and upper structure on running kinematics and kinetics of non-professional runners by using feature selection, information gain, and artificial neural network analysis...
2017: PeerJ
https://www.readbyqxmd.com/read/28247569/transcatheter-aortic-valve-implantation-in-the-united-states-predictors-of-early-hospital-discharge
#5
Sagar Mallikethi-Reddy, Emmanuel Akintoye, Tesfaye Telila, Rajeev Sudhakar, Kavyashri Jagadeesh, Alexandros Briasoulis, Melvyn Rubenfire, Luis Afonso, Cindy L Grines
BACKGROUND: There is a concerted push for adopting a minimalist strategy with emphasis on early hospital discharge for patients undergoing Transcatheter aortic valve implantation (TAVI). However, studies on discharge patterns and predictors of early discharge (≤3 days post-TAVI) are sparse, in the United States. METHODS: We analyzed using Healthcare Utilization Project, Nationwide Inpatient Sample database, 2011-2012. A total of 7321 TAVI procedures were identified...
March 1, 2017: Journal of Interventional Cardiology
https://www.readbyqxmd.com/read/28242585/at-least-7-days-delayed-stenting-using-minimalist-immediate-mechanical-intervention-mimi-in-st-segment-elevation-myocardial-infarction-the-super-mimi-study
#6
Petru Mester, Helene Bouvaist, Nicolas Delarche, Frédéric Bouisset, Mohamed Abdellaoui, Pierre-Yves Petiteau, Olivier Dubreuil, Ziad Boueri, Mohamed Chettibi, Géraud Souteyrand, Hend Madiot, Loic Belle
AIMS: To ascertain whether a minimalist immediate mechanical intervention (MIMI) aiming to restore an optimal Thrombolysis In Myocardial Infarction (TIMI) flow in the culprit artery, followed ≥7 days later by a second percutaneous coronary intervention with intentional stenting is safe in patients with ST- segment elevation myocardial infarction and large thrombotic burden. METHODS AND RESULTS: SUPER-MIMI was a prospective, observational trial conducted between January 2014 and April 2015 in 14 French centres...
February 28, 2017: EuroIntervention
https://www.readbyqxmd.com/read/28223128/a-screening-assay-for-the-identification-of-host-cell-requirements-and-antiviral-targets-for-hepatitis-d-virus-infection
#7
Bettina Buchmann, Katinka Döhner, Thomas Schirdewahn, Beate Sodeik, Michael P Manns, Heiner Wedemeyer, Sandra Ciesek, Thomas von Hahn
Hepatitis delta virus (HDV) is a minimalistic satellite virus of hepatitis B virus (HBV). HBV/HDV co-infection, i.e. "hepatitis D", is the most severe form of viral hepatitis. No effective therapy for HDV infection is available partly due to the fact that HDV is a highly host-dependent virus devoid of any potentially drugable enzyme encoded in its small genome. In this study we present a semi-automated method to evaluate HDV infection and replication under the influence of different drugs. We utilized a Huh-7/hNTCP cell culture based system in a 96-well plate format, an automated microscope and image acquisition as well as analysis with the CellProfiler software to quantify the impact of these drugs on HDV infection...
February 20, 2017: Antiviral Research
https://www.readbyqxmd.com/read/28208479/self-tuning-bistable-parametric-feedback-oscillator-near-optimal-amplitude-maximization-without-model-information
#8
David J Braun, Andrius Sutas, Sethu Vijayakumar
Theory predicts that parametrically excited oscillators, tuned to operate under resonant condition, are capable of large-amplitude oscillation useful in diverse applications, such as signal amplification, communication, and analog computation. However, due to amplitude saturation caused by nonlinearity, lack of robustness to model uncertainty, and limited sensitivity to parameter modulation, these oscillators require fine-tuning and strong modulation to generate robust large-amplitude oscillation. Here we present a principle of self-tuning parametric feedback excitation that alleviates the above-mentioned limitations...
January 2017: Physical Review. E
https://www.readbyqxmd.com/read/28196587/pheochromocytomas-and-paragangliomas-genetically-diverse-and-minimalist-all-at-once
#9
Patricia L M Dahia
Pheochromocytomas and paragangliomas are infrequent, genetically heterogeneous neuroendocrine tumors. In this issue of Cancer Cell, Fishbein et al. report novel driver genes, pathogenic mechanisms, and markers of malignancy. Notably, the new findings support the long-held view that these tumors distinctively arise from a single driver event, either inherited or acquired.
February 13, 2017: Cancer Cell
https://www.readbyqxmd.com/read/28164007/minimalist-immediate-mechanical-intervention-in-acute-st-segment-elevation-myocardial-infarction-is-it-time-to-redefine-targets
#10
EDITORIAL
Philip Francis Dingli, Javier Escaned
No abstract text is available yet for this article.
February 2017: Cardiovascular Diagnosis and Therapy
https://www.readbyqxmd.com/read/28155642/statistical-ensemble-of-gene-regulatory-networks-of-macrophage-differentiation
#11
Filippo Castiglione, Paolo Tieri, Alessandro Palma, Abdul Salam Jarrah
BACKGROUND: Macrophages cover a major role in the immune system, being the most plastic cell yielding several key immune functions. METHODS: Here we derived a minimalistic gene regulatory network model for the differentiation of macrophages into the two phenotypes M1 (pro-) and M2 (anti-inflammatory). RESULTS: To test the model, we simulated a large number of such networks as in a statistical ensemble. In other words, to enable the inter-cellular crosstalk required to obtain an immune activation in which the macrophage plays its role, the simulated networks are not taken in isolation but combined with other cellular agents, thus setting up a discrete minimalistic model of the immune system at the microscopic/intracellular (i...
December 22, 2016: BMC Bioinformatics
https://www.readbyqxmd.com/read/28149567/minimalist-video-assisted-thoracic-surgery-biopsy-of-mediastinal-tumors
#12
Federico Tacconi, Paola Rogliani, Benedetto Cristino, Francesco Gilardi, Leonardo Palombi, Eugenio Pompeo
BACKGROUND: Mediastinal tumors often require surgical biopsy to achieve a precise and rapid diagnosis. However, subjects with mediastinal tumors may be unfit for general anesthesia, particularly when compression of major vessels or airways does occur. We tested the applicability in this setting of a minimalist (M) uniportal, video-assisted thoracic surgery (VATS) strategy carried out under locoregional anesthesia in awake patients (MVATS). METHODS: We analyzed in a comparative fashion including propensity score matching, data from a prospectively collected database of patients who were offered surgical biopsy for mediastinal tumors through either MVATS or standard VATS...
December 2016: Journal of Thoracic Disease
https://www.readbyqxmd.com/read/28129518/body-mass-and-weekly-training-distance-influence-the-pain-and-injuries-experienced-by-runners-using-minimalist-shoes
#13
Joel T Fuller, Dominic Thewlis, Jonathan D Buckley, Nicholas A T Brown, Joseph Hamill, Margarita D Tsiros
BACKGROUND: Minimalist shoes have been popularized as a safe alternative to conventional running shoes. However, a paucity of research is available investigating the longer-term safety of minimalist shoes. PURPOSE: To compare running-related pain and injury between minimalist and conventional shoes in trained runners and to investigate interactions between shoe type, body mass, and weekly training distance. STUDY DESIGN: Randomized clinical trial; Level of evidence, 2...
January 1, 2017: American Journal of Sports Medicine
https://www.readbyqxmd.com/read/28128389/synthetic-minimalistic-tryptophan-zippers-as-a-chiroptical-switch
#14
V Haridas, Sandhya Sadanandan, Sameer Dhawan, Rituraj Mishra, Ishani Jain, Gaurav Goel, Yuan Hu, Sandeep Patel
In this paper, we presented a new design strategy for a peptide-based chiral supramolecular assembly. A series of aryl linked peptides 1a-1f were designed and synthesized. The bis-urea peptides 1a-1c self-assembled into a helical supramolecular arrangement resembling Trp zipper (Trpzip) structures present in proteins. Interestingly, a dihydrogenphosphate anion, upon binding to the assembly, could invert the chirality of the supramolecular assembly which could be reverted to the original by the addition of water...
January 27, 2017: Organic & Biomolecular Chemistry
https://www.readbyqxmd.com/read/28114020/indoor-scene-reconstruction-using-near-light-photometric-stereo
#15
Jingtang Liao, Bert Buchholz, Jean-Marc Thiery, Pablo Bauszat, Elmar Eisemann
We propose a novel framework for photometric stereo (PS) under low-light conditions using uncalibrated nearlight illumination. It operates on free-form video sequences captured with a minimalistic and affordable setup. We address issues such as albedo variations, shadowing, perspective projections and camera noise. Our method uses specular spheres detected with a perspective-correcting Hough transform to robustly triangulate light positions in the presence of outliers via a least-squares approach. Furthermore, we propose an iterative reweighting scheme in combination with an `p-norm minimizer to robustly solve the calibrated near-light PS problem...
December 7, 2016: IEEE Transactions on Image Processing: a Publication of the IEEE Signal Processing Society
https://www.readbyqxmd.com/read/28100647/marpodb-an-open-registry-for-marchantia-polymorpha-genetic-parts
#16
Mihails Delmans, Bernardo Pollak, Jim Haseloff
Marchantia polymorpha is an extant relative of the earliest terrestrial plants and has attracted a substantial interest as a model organism for evolutionary and developmental studies. Given its relatively simple genome, compact gene families, simple morphology, ease of propagation and transformation, M. polymorpha is becoming a promising platform for plant synthetic biology. Modular genetic parts have been essential for development of synthetic biology approaches, so we sought to design an engineering oriented database for M...
January 18, 2017: Plant & Cell Physiology
https://www.readbyqxmd.com/read/28054630/disaggregation-of-amylin-aggregate-by-novel-conformationally-restricted-aminobenzoic-acid-containing-%C3%AE-%C3%AE-and-%C3%AE-%C3%AE-hybrid-peptidomimetics
#17
Ashim Paul, Sourav Kalita, Sujan Kalita, Piruthivi Sukumar, Bhubaneswar Mandal
Diabetes has emerged as a threat to the current world. More than ninety five per cent of all the diabetic population has type 2 diabetes mellitus (T2DM). Aggregates of Amylin hormone, which is co-secreted with insulin from the pancreatic β-cells, inhibit the activities of insulin and glucagon and cause T2DM. Importance of the conformationally restricted peptides for drug design against T2DM has been invigorated by recent FDA approval of Symlin, which is a large conformationally restricted peptide. However, Symlin still has some issues including solubility, oral bioavailability and cost of preparation...
January 5, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28051921/tube-thoracostomy-at-the-time-of-congenital-diaphragmatic-hernia-repair-reassessing-the-risks-and-benefits
#18
Avraham Schlager, Kelly Arps, Ragavan Siddharthan, Matthew S Clifton
PURPOSE: Postoperative pneumothorax and effusion remain a concern following congenital diaphragmatic hernia (CDH) repair. Despite a recent trend away from intraoperative thoracostomy, few studies have actually compared outcomes with and without a chest tube. Rationale commonly cited for the more minimalistic approach include the presumed low likelihood of postoperative complications, potential risk of patch infection, and prolonged intubation. We evaluate these theories, as well as the implications of intraoperative chest tube (IOCT) placement...
March 2017: Journal of Laparoendoscopic & Advanced Surgical Techniques. Part A
https://www.readbyqxmd.com/read/28046434/su-f-t-458-tracking-trends-of-tg-142-parameters-via-analysis-of-data-recorded-by-2d-chamber-array
#19
A Alexandrian, C Kabat, D Defoor, D Saenz, K Rasmussen, N Kirby, A Gutierrez, N Papanikolaou, S Stathakis
PURPOSE: With increasing QA demands of medical physicists in clinical radiation oncology, the need for an effective method of tracking clinical data has become paramount. A tool was produced which scans through data automatically recorded by a 2D chamber array and extracts relevant information recommended by TG-142. Using this extracted information a timely and comprehensive analysis of QA parameters can be easily performed enabling efficient monthly checks on multiple linear accelerators simultaneously...
June 2016: Medical Physics
https://www.readbyqxmd.com/read/28025343/reefgenomics-org-a-repository-for-marine-genomics-data
#20
Yi Jin Liew, Manuel Aranda, Christian R Voolstra
Over the last decade, technological advancements have substantially decreased the cost and time of obtaining large amounts of sequencing data. Paired with the exponentially increased computing power, individual labs are now able to sequence genomes or transcriptomes to investigate biological questions of interest. This has led to a significant increase in available sequence data. Although the bulk of data published in articles are stored in public sequence databases, very often, only raw sequencing data are available; miscellaneous data such as assembled transcriptomes, genome annotations etc...
2016: Database: the Journal of Biological Databases and Curation
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