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https://www.readbyqxmd.com/read/27903913/functional-rnas-combined-assembly-and-packaging-in-vlps
#1
Po-Yu Fang, Lizzette M Gómez Ramos, Stefany Y Holguin, Chiaolong Hsiao, Jessica C Bowman, Hung-Wei Yang, Loren Dean Williams
We describe here a one pot RNA production, packaging and delivery system based on bacteriophage Qβ. We demonstrate a method for production of a novel RNAi scaffold, packaged within Qβ virus-like particles (VLPs). The RNAi scaffold is a general utility chimera that contains a functional RNA duplex with paired silencing and carrier sequences stabilized by a miR-30 stem-loop. The Qβ hairpin on the 5' end confers affinity for the Qβ coat protein (CP). Silencing sequences can include mature miRNAs and siRNAs, and can target essentially any desired mRNA...
November 29, 2016: Nucleic Acids Research
https://www.readbyqxmd.com/read/27903358/-comparison-of-genotypes-of-hepatitis-c-virus-between-chinese-and-burmese-drug-users-living-in-dehong-prefecture-yunnan-province
#2
Y K Wang, K D Feng, J B Wang, Z M Zhang, R H Tang, R H Ye, J N Zhang, Y C Yang, M F Qiu, S Duan
Objective: To analyze the distribution of hepatitis C virus (HCV) genotypes among Chinese and Burmese drug users in Dehong Prefecture, Yunnan Province. Methods: Plasma specimens and relevant epidemiological data were collected from 7 545 drug users attending rehabilitation centers and methadone clinics in Dehong Prefecture from January to September in 2015, of which 752 were positive for HCV antibodies. HCV RNA was then extracted from 139 random specimens (64 Burmese and 75 Chinese) and the CE1 and NS5B regions were amplified by nested PCR...
November 6, 2016: Zhonghua Yu Fang Yi Xue za Zhi [Chinese Journal of Preventive Medicine]
https://www.readbyqxmd.com/read/27902874/spodoptera-litura-autophagy-related-protein-1-interacts-with-autophagy-related-protein-5-and-enhances-its-degradation
#3
N Zhang, Y Yang, H Lu, Y Xiang, X Huang, R Hu, Z Chen, W Yuan, R Peng, J Peng, H Ai, K Liu
It is known that the autophagy-related protein 1 (ATG1) plays critical roles in the regulation of autophagy in mammals and yeast, whereas the function of ATG1 in lepidopteran insects is not well elucidated. Here Spodoptera litura ATG1 (SlATG1) and its interactions with other ATG proteins were characterized. Alternative splicing of SlAtg1 produced at least four transcript variants. Over-expression and RNA interference knockdown of SlAtg1 demonstrated that SlATG1 enhanced autophagy. SlATG1A-Green fluorescent protein (GFP) tagged localized in the cytoplasm and formed some punctuate dots, which were colocalized with red fluorescent protein mCherry tagged Spodoptera exigua ATG5 (SeATG5)...
November 30, 2016: Insect Molecular Biology
https://www.readbyqxmd.com/read/27901642/a-generic-rna-pulsed-dc-based-approach-for-developing-therapeutic-intervention-against-nasopharyngeal-carcinoma
#4
Rajeev K Tyagi, Rajesh Parmar, Naisargee Patel
The recurrent nasopharyngeal carcinoma of head-and-neck cancers pathology showed unique symptoms and clinical characteristics. The complexity of pathology poses challenges for developing therapeutic interventional approaches against nasopharyngeal carcinoma (NPC). The conventional treatment regimens offer limited local control and survival, which, leads to adverse delayed complications. Our study present a generic monocyte derived dendritic cell (MoDC) vaccine strategy for NPC in which RNA is used as a source of tumor-associated antigens (TAAgs)...
November 30, 2016: Human Vaccines & Immunotherapeutics
https://www.readbyqxmd.com/read/27901018/the-signaling-axis-of-microrna-31-interleukin-25-regulates-th1-th17-mediated-inflammation-response-in-colitis
#5
T Shi, Y Xie, Y Fu, Q Zhou, Z Ma, J Ma, Z Huang, J Zhang, J Chen
Interleukin-25 (IL-25) is an important regulatory cytokine that has a key role on mucosal immune tolerance during inflammation response. However, the molecular mechanism that regulates the colonic IL-25 expression in Crohn's disease (CD) remains unclear. In this study, IL-25 level was proved to decrease in 2,4,6-trinitrobenzene sulfonic acid (TNBS)-induced colitis mice and IL-10 knockout (KO) spontaneous colitis mice. An inverse correlation between IL-25 and miR-31 was discovered in the colons from model mice and CD patients...
November 30, 2016: Mucosal Immunology
https://www.readbyqxmd.com/read/27895107/long-noncoding-rna-h19-accelerates-tenogenic-differentiation-and-promotes-tendon-healing-through-targeting-mir-29b-3p-and-activating-tgf-%C3%AE-1-signaling
#6
Ying-Fei Lu, Yang Liu, Wei-Ming Fu, Jia Xu, Bin Wang, Yu-Xin Sun, Tian-Yi Wu, Liang-Liang Xu, Kai-Ming Chan, Jin-Fang Zhang, Gang Li
Tendon injures are common orthopedic conditions, but tendon development and the pathogenesis of tendon injures, such as tendinopathy, remain largely unknown and have limited the development of clinical therapy. Studies on tenogenic differentiation at the molecular level may help in developing novel therapeutic strategies. As novel regulators, long noncoding RNAs (lncRNAs) have been found to have widespread biological functions, and emerging evidence demonstrates that lncRNAs may play important regulatory roles in cell differentiation and tissue regeneration...
November 28, 2016: FASEB Journal: Official Publication of the Federation of American Societies for Experimental Biology
https://www.readbyqxmd.com/read/27892920/foxk1-interaction-with-fhl2-promotes-proliferation-invasion-and-metastasis-in-colorectal-cancer
#7
M Wu, J Wang, W Tang, X Zhan, Y Li, Y Peng, X Huang, Y Bai, J Zhao, A Li, C Chen, Y Chen, H Peng, Y Ren, G Li, S Liu, J Wang
The transcriptional factor Forkhead box k1 (FOXK1) is a member of the FOX family. The abnormal expression of FOXK1 may have an important role in tumour development. Our previous studies showed that four-and-a-half LIM protein 2 (FHL2) is a critical inducer of the epithelial-to-mesenchymal transition (EMT) and invasion. However, the molecular mechanism by which FOXK1 synergizes with FHL2 tumour proliferation, EMT and metastasis is not well defined. We evaluated that messenger RNA (mRNA) and protein expression levels by quantitative RT-PCR, western blot, immunofluorescence and immunohistochemistry (IHC) assays...
November 28, 2016: Oncogenesis
https://www.readbyqxmd.com/read/27889920/neuropeptide-y-receptor-1-is-expressed-by-b-and-t-lymphocytes-and-mast-cells-in-infantile-haemangiomas
#8
Elysia M S Tan, Max G Blackwell, Jonathan C Dunne, Reginald Marsh, Swee T Tan, Tinte Itinteang
AIM: We investigated the expression of neuropeptide Y (NPY), NPY receptor 1 (NPYR1) and NPY receptor 2 (NPYR2) in infantile haemangiomas (IHs). METHODS: Immunohistochemical (IHC) staining was performed on proliferating IHs from six patients aged 4-13 (mean 8.7) months and involuted IHs from six patients aged 5-59 (mean 18.7) years, for the expression of NPY, NPYR1 and NPYR2. Protein and messenger ribonucleic acid expression corresponding to these proteins was investigated by Western blotting and NanoString analysis, respectively...
November 27, 2016: Acta Paediatrica
https://www.readbyqxmd.com/read/27889163/differential-detection-of-pathogenic-yersinia-spp-by-fluorescence-in-situ-hybridization
#9
Alexander Rohde, Jens Andre Hammerl, Bernd Appel, Ralf Dieckmann, Sascha Al Dahouk
Yersinia enterocolitica, Y. pseudotuberculosis and Y. pestis are pathogens of major medical importance, which are responsible for a considerable number of infections every year. The detection of these species still relies on cultural methods, which are slow, labour intensive and often hampered by the presence of high amounts of accompanying flora. In this study, fluorescence in situ hybridization (FISH) was used to develop a fast, sensitive and reliable alternative to detect viable bacteria in food. For this purpose, highly specific probes targeting the 16S and 23S ribosomal RNA were employed to differentially detect each of the three species...
April 2017: Food Microbiology
https://www.readbyqxmd.com/read/27886335/microrna-421-induces-apoptosis-of-c-33a-cervical-cancer-cells-via-down-regulation-of-bcl-xl
#10
X J Lai, X Y Cheng, L D Hu
Cervical cancer is a life-threatening condition. MicroRNAs (miRNAs) can promote or inhibit cell death and proliferation. The present study investigated the effect of miRNA 421 on the growth and apoptosis of cervical cancer cells. miRNA 421 and control miRNA were synthesized and transfected into c-33a cervical cancer cells. A thiazolyl blue tetrazolium bromide assay, caspase-3 activity, and flow cytometry were used to study the effects of miRNA 421 on c-33a cell growth, and apoptosis. Small interfering RNA targeting Bcl-xL was synthesized and transfected into c-33a cells along with miRNA 421...
November 21, 2016: Genetics and Molecular Research: GMR
https://www.readbyqxmd.com/read/27884156/targeted-myocardial-gene-expression-in-failing-hearts-by-rna-sequencing
#11
Kajari Dhar, Alexandra M Moulton, Eric Rome, Fang Qiu, Jeff Kittrell, Eugenia Raichlin, Ronald Zolty, John Y Um, Michael J Moulton, Hesham Basma, Daniel R Anderson, James D Eudy, Brian D Lowes
BACKGROUND: Myocardial recovery with left ventricular assist device (LVAD) therapy is highly variable and difficult to predict. Next generation ribonucleic acid (RNA) sequencing is an innovative, rapid, and quantitative approach to gene expression profiling in small amounts of tissue. Our primary goal was to identify baseline transcriptional profiles in non-ischemic cardiomyopathies that predict myocardial recovery in response to LVAD therapy. We also sought to verify transcriptional differences between failing and non-failing human hearts...
November 25, 2016: Journal of Translational Medicine
https://www.readbyqxmd.com/read/27881660/the-norovirus-ns3-protein-is-a-dynamic-lipid-and-microtubule-associated-protein-involved-in-viral-rna-replication
#12
Ben Cotton, Jennifer L Hyde, Soroush T Sarvestani, Stanislav V Sosnovtsev, Kim Y Green, Peter A White, Jason M Mackenzie
: Norovirus (NoV) infections are a significant health burden to society yet the lack of reliable tissue culture systems has hampered the development of appropriate antiviral therapies. Here we show that the NoV NS3 protein, derived from murine NoV (MNV), is intimately associated with the MNV replication complex and the viral replication intermediate dsRNA. We have observed that when expressed individually MNV NS3 and NS3 encoded by human Norwalk virus (NV) induced the formation of distinct vesicle-like structures that did not co-localise with any particular protein markers to cellular organelles, but localised to cellular membranes, in particular those high in cholesterol content...
November 23, 2016: Journal of Virology
https://www.readbyqxmd.com/read/27881532/genome-sequence-of-mycobacteriophage-erniej
#13
Courtney J Robinson, Laricca Y London-Thomas, Leon A Dickson, Tiffany A Clinton, Hana Baig, Maude Bute, Mohammed Fahad, Kanhai Farrakhan, Neshaun Grady, Nicholas E Guthrie, Ruoa Hafid, Jayla Harvey, Kellie Hunnicutt, Victoria L Larsen, Taashaylaray McDuffie, Earyn N McGee, Jillian Y Pailin, Bria Peacock, Antolice Thomas, Winston A Anderson
ErnieJ, a cluster C mycobacteriophage that infects Mycobacterium smegmatis mc(2)155, was recovered from soil in Washington, DC. Its genome is 153,243 bp in size and encodes 227 predicted proteins, 30 tRNAs, and one transfer-messenger RNA (tmRNA). Ten percent of the predicted proteins have homologs in phages that infect nonmycobacterial Actinobacteria.
November 23, 2016: Genome Announcements
https://www.readbyqxmd.com/read/27881440/culturing-bone-marrow-cells-with-dexamethasone-and-ascorbic-acid-improves-osteogenic-cell-sheet-structure
#14
M Akahane, T Shimizu, T Kira, T Onishi, Y Uchihara, T Imamura, Y Tanaka
OBJECTIVES: To assess the structure and extracellular matrix molecule expression of osteogenic cell sheets created via culture in medium with both dexamethasone (Dex) and ascorbic acid phosphate (AscP) compared either Dex or AscP alone. METHODS: Osteogenic cell sheets were prepared by culturing rat bone marrow stromal cells in a minimal essential medium (MEM), MEM with AscP, MEM with Dex, and MEM with Dex and AscP (Dex/AscP). The cell number and messenger (m)RNA expression were assessed in vitro, and the appearance of the cell sheets was observed after mechanical retrieval using a scraper...
November 2016: Bone & Joint Research
https://www.readbyqxmd.com/read/27879433/comparison-of-shape-reagents-for-mapping-rna-structures-inside-living-cells
#15
Byron Lee, Ryan Alexander Flynn, Anastasia Kadina, Jimmy K Guo, Eric T Kool, Howard Y Chang
Recent advances in SHAPE technology have converted the classic primer extension method to next generation sequencing platforms, allowing transcriptome-level analysis of RNA secondary structure. In particular, icSHAPE and SHAPE-MaP, using NAI-N3 and 1M7 reagents respectively, are methods that claim to measure in vivo structure with high-throughput sequencing. However, these compounds have not been compared on an unbiased, raw-signal level. Here, we directly compare several in vivo SHAPE acylation reagents using the simple primer extension assay...
November 22, 2016: RNA
https://www.readbyqxmd.com/read/27876297/host-transcriptomic-responses-to-pneumonic-plague-reveal-that-yersinia-pestis-inhibits-both-the-initial-adaptive-and-innate-immune-responses-in-mice
#16
Huiying Yang, Tong Wang, Guang Tian, Qingwen Zhang, Xiaohong Wu, Youqian Xin, Yanfeng Yan, Yafang Tan, Shiyang Cao, Wanbing Liu, Yujun Cui, Ruifu Yang, Zongmin Du
Pneumonic plague is the most deadly form of infection caused by Yersinia pestis and can progress extremely fast. However, our understanding on the host transcriptomic response to pneumonic plague is insufficient. Here, we used RNA-sequencing technology to analyze transcriptomic responses in mice infected with fully virulent strain 201 or EV76, a live attenuated vaccine strain lacking the pigmentation locus. Approximately 600 differentially expressed genes (DEGs) were detected in lungs from both 201- and EV76-infected mice at 12h post-infection (hpi)...
November 14, 2016: International Journal of Medical Microbiology: IJMM
https://www.readbyqxmd.com/read/27874956/up-regulation-of-long-non-coding-rna-bcar4-predicts-a-poor-prognosis-in-patients-with-osteosarcoma-and-promotes-cell-invasion-and-metastasis
#17
L Ju, Y-M Zhou, G-S Yang
OBJECTIVE: The long non-coding RNA BCAR4 (BCAR4) has been reported to be associated with cancer development. The aim of our study was to investigate the expression of BCAR4 in osteosarcoma patients and its association with clinicopathologic parameters and the prognosis. PATIENTS AND METHODS: Quantitative RT-PCR (qRT-PCR) assay was used to detect the expression of BCAR4 and its correlations with clinicopathological factors were statistically analyzed. The clinical and prognostic significance of BCAR4 expression was analyzed statistically by Kaplan-Meier estimate and Cox regression model...
November 2016: European Review for Medical and Pharmacological Sciences
https://www.readbyqxmd.com/read/27874951/high-expression-of-long-noncoding-rna-sox2ot-is-associated-with-the-aggressive-progression-and-poor-outcome-of-gastric-cancer
#18
J-H Zou, C-Y Li, J Bao, G-Q Zheng
OBJECTIVE: The aim of our study was to investigate the expression of long non-coding RNA Sox2ot (Sox2ot) in gastric cancer (GC) patients and its association with clinicopathologic parameters and the prognosis. PATIENTS AND METHODS: Quantitative real-time polymerase chain reaction was performed to detect Sox2ot expression in 155 GC tissues and paired adjacent normal tissues. The relationships between Sox2ot expression and the clinicopathological features of GC patients were analyzed...
November 2016: European Review for Medical and Pharmacological Sciences
https://www.readbyqxmd.com/read/27874855/conditional-control-of-suicide-gene-expression-in-tumor-cells-with-theophylline-responsive-ribozyme
#19
Y Zhang, J Wang, H Cheng, Y Sun, M Liu, Z Wu, R Pei
Numerous synthetic RNA-based controls for integrating sensing switches with function devices have been demonstrated in a variety of organisms for gene regulatory. Although potential advantages of RNA-based genetic control strategies have been shown in clinical applications, successful extending these engineered systems into medical applications has seldom reported. Here, a synthetic RNA-based ribozyme system and its application in advancing rationally designed cellular therapy were described. The theophylline-responsive ribozyme-based device provided a powerful platform for suicide gene expression regulation in tumor cells...
November 22, 2016: Gene Therapy
https://www.readbyqxmd.com/read/27873335/lip-b-1-is-a-novel-allergenic-protein-isolated-from-the-booklouse-liposcelis-bostrychophila
#20
O Ishibashi, K Sakuragi, Y Fukutomi, Y Kawakami, Y Kamata, M Sakurai, S Nakayama, H Uchiyama, H Kobayashi, H Kojima, T Inui
BACKGROUND: Booklice, belonging to the order Psocoptera, are small household insect pests that are distributed worldwide. Liposcelis bostrychophila (L. bostrychophila), a common home-inhabiting species of booklouse, infests old books, sheets of paper and stored food. Recent entomological and serological studies demonstrated that L. bostrychophila accounted for the majority of detectable insects in house dust and could be a potent inducer of respiratory allergy. Our recent proteomic analysis identified a potent allergenic protein from L...
November 22, 2016: Allergy
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