keyword
MENU ▼
Read by QxMD icon Read
search

5-ALA

keyword
https://www.readbyqxmd.com/read/28346389/artesunate-enhances-the-cytotoxicity-of-5-aminolevulinic-acid-based-sonodynamic-therapy-against-mouse-mammary-tumor-cells-in-vitro
#1
Tomohiro Osaki, Yoshihiro Uto, Masahiro Ishizuka, Tohru Tanaka, Nobuyasu Yamanaka, Tsukasa Kurahashi, Kazuo Azuma, Yusuke Murahata, Takeshi Tsuka, Norihiko Itoh, Tomohiro Imagawa, Yoshiharu Okamoto
Sonodynamic therapy (SDT) kills tumor cells through the synergistic effects of ultrasound (US) and a sonosensitizer agent. 5-Aminolevulinic acid (5-ALA) has been used as a sonodynamic sensitizer for cancer treatment. However, studies have shown that 5-ALA-based SDT has limited efficacy against malignant tumors. In this study, we examined whether artesunate (ART) could enhance the cytotoxicity of 5-ALA-based SDT against mouse mammary tumor (EMT-6) cells in vitro. In the ART, ART + US, ART + 5-ALA, and ART + 5-ALA + US groups, the cell survival rate correlated with ART concentration, and decreased with increasing concentrations of ART...
March 27, 2017: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/28338432/dual-labeling-with-5-aminolevulinic-acid-and-fluorescein-for-fluorescence-guided-resection-of-high-grade-gliomas-technical-note
#2
Eric Suero Molina, Johannes Wölfer, Christian Ewelt, André Ehrhardt, Benjamin Brokinkel, Walter Stummer
OBJECTIVE Fluorescence guidance with 5-aminolevulinic acid (5-ALA) helps improve resections of malignant gliomas. However, one limitation is the low intensity of blue light for background illumination. Fluorescein has recently been reintroduced into neurosurgery, and novel microscope systems are available for visualizing this fluorochrome, which highlights all perfused tissues but has limited selectivity for tumor detection. Here, the authors investigate a combination of both fluorochromes: 5-ALA for distinguishing tumor and fluorescein for providing tissue fluorescence of adjacent brain tissue...
March 24, 2017: Journal of Neurosurgery
https://www.readbyqxmd.com/read/28338177/5-aminolevulinic-acid-photodynamic-therapy-stimulates-local-immunity-in-patients-with-condylomata-acuminata-via-activation-of-t-lymphocytes
#3
J Du, Q Cheng, Z Zhang, J-F Wu, F Li, S-Y Chen, Y-L Wang, X-N Lu, J-H Xu
OBJECTIVE: 5-Aminolevulinic acid photodynamic therapy (5-ALA-PDT) for condylomata acuminate (CA) is effective and safe, but how this treatment affects immune reaction is poorly understood. We aimed to explore the effects of PDT on local immunity in CA. PATIENTS AND METHODS: Immunohistochemical analysis before and after PDT was performed to analyze local changes in the distribution of T lymphocytes, CD123+ plasmacytoid dendritic cells (pDCs) and CD1a+ myeloid DCs...
March 2017: European Review for Medical and Pharmacological Sciences
https://www.readbyqxmd.com/read/28321653/5-aminolevulinic-acid-modulates-antioxidant-defense-systems-and-mitigates-drought-induced-damage-in-kentucky-bluegrass-seedlings
#4
Kuiju Niu, Xiang Ma, Guoling Liang, Huiling Ma, Zhifeng Jia, Wenhui Liu, Qianqian Yu
Drought stress occurs frequently and severely as a result of global climate change, and it exerts serious effects on plants. 5-Aminolevulinic acid (5-ALA) plays a crucial role in conferring abiotic stress tolerance in plants. To enhance the drought tolerance of turfgrass and investigate the effects of 5-ALA on antioxidant metabolism and gene expression under drought stress conditions, exogenous 5-ALA was applied by foliar spraying before Kentucky bluegrass (Poa pratensis L.) seedlings were exposed to drought [induced by 10% polyethylene glycol (PEG)] stress for 20 days...
March 20, 2017: Protoplasma
https://www.readbyqxmd.com/read/28292933/complete-elucidation-of-the-late-steps-of-bafilomycin-biosynthesis-in-streptomyces-lohii
#5
Zhong Li, Lei Du, Wei Zhang, Xingwang Zhang, Yuanyuan Jiang, Kun Liu, Ping Men, Huifang Xu, Jeffrey L Fortman, David H Sherman, Bing Yu, Song Gao, Shengying Li
Bafilomycins are an important subgroup of polyketides with diverse biological activities and possible applications as specific inhibitors of vacuolar H+-ATPase. However, the general toxicity and structural complexity of bafilomycins present formidable challenges to drug design via chemical modification, prompting interests in improving bafilomycin activities via biosynthetic approaches. Two bafilomycin biosynthetic gene clusters have been identified, but their post- polyketide synthase (PKS) tailoring steps for structural diversification and bioactivity improvement remain largely unknown...
March 14, 2017: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/28291213/-intraoperative-fluorescence-diagnosis-using-5-aminolevulinic-acid-in-surgical-treatment-of-children-with-recurrent-neuroepithelial-tumors
#6
A V Kim, V A Khachatryan
AIM: To analyze the efficacy and safety of 5-aminolevulinic acid for intraoperative fluorescence diagnosis (FD) during repeated surgery for recurrent neuroepithelial tumors (NETs) in children. MATERIAL AND METHODS: We performed a retrospective analysis of the results of recurrent NET resection in 13 children aged from 3 to 17 years in the period between December 2013 and May 2015. The source of 5-ALA was a drug "Alasens" that was administered at a dose of 20 mg/kg of body weight 4 h before the beginning of tumor resection...
2017: Zhurnal Voprosy Neĭrokhirurgii Imeni N. N. Burdenko
https://www.readbyqxmd.com/read/28265818/the-patients-view-impact-of-the-extent-of-resection-intraoperative-imaging-and-awake-surgery-on-health-related-quality-of-life-in-high-grade-glioma-patients-results-of-a-multicenter-cross-sectional-study
#7
Katrin Nickel, Mirjam Renovanz, Jochem König, Linda Stöckelmaier, Anne-Katrin Hickmann, Minou Nadji-Ohl, Jens Engelke, Elke Weimann, Dirk Freudenstein, Oliver Ganslandt, Lars Bullinger, Christian Rainer Wirtz, Jan Coburger
The objective of the present study is to assess the influence of extent of resection (EoR), use of intraoperative imaging, and awake surgery on health-related quality of life (HRQoL) in high-grade glioma (HGG) patients in a prospective multicenter study. We analyzed 170 surgeries of patients suffering from a HGG. During the first year after resection, HRQoL was evaluated using the European Organization of Research and Treatment of Cancer Core Questionnaire C30 and Brain Neoplasm 20 questionnaires. We assessed the influence of EoR; awake surgery; and use of 5-aminolevulinic acid (5-ALA), intraoperative MRI (iMRI), and their combination on sum scores for function and symptoms as well as several neurological single items...
March 6, 2017: Neurosurgical Review
https://www.readbyqxmd.com/read/28254552/assessment-of-in-vivo-experiments-the-newly-synthesized-porphyrin-with-proper-light-source-enhanced-effectiveness-of-pdt-comparing-to-5-ala
#8
Kamil Jurczyszyn, Marta Woźniak, Krzysztof Symonowicz, Natasza Sprutta, Lechosław Latos-Grażyński, Piotr Ziółkowski, Yurij Ivonyak, Piotr Tuchowski, Witold Trzeciakowski
BACKGROUND: The search for new photosensitizers for application in photodynamic therapy has quite a long history. In the past, a large number of potent photosensitizers were used in both basic and clinical studies; however, only a few turned out to be effective and safe. METHODS: In the present study, two compounds were used: 5-aminolevulinic acid in two formulations (free and liposomal), and the newly synthesized porphyrin, 5,10,15,20-tetra-p-tolyl-22,24-dithiadibenzocarbaporphyrin, termed DTDB...
February 27, 2017: Photodiagnosis and Photodynamic Therapy
https://www.readbyqxmd.com/read/28223144/combination-of-hand-held-probe-and-microscopy-for-fluorescence-guided-surgery-in-the-brain-tumor-marginal-zone
#9
Johan Richter, Neda Haj-Hosseini, Martin Hallbeck, Karin Wårdell
BACKGROUND: Visualization of the tumor is crucial for differentiating malignant tissue from healthy brain during surgery, especially in the tumor marginal zone. The aim of the study was to introduce a fluorescence spectroscopy-based hand-held probe (HHF-probe) for tumor identification in combination with the fluorescence guided resection surgical microscope (FGR-micsorcope), and evaluate them in terms of diagnostic performance and practical aspects of fluorescence detection. METHODS: Eighteen operations were performed on 16 patients with suspected high-grade glioma...
February 18, 2017: Photodiagnosis and Photodynamic Therapy
https://www.readbyqxmd.com/read/28169355/enhancement-of-5-aminolevulinic-acid-based-fluorescence-detection-of-side-population-defined-glioma-stem-cells-by-iron-chelation
#10
Wenqian Wang, Kouichi Tabu, Yuichiro Hagiya, Yuta Sugiyama, Yasuhiro Kokubu, Yoshitaka Murota, Shun-Ichiro Ogura, Tetsuya Taga
Cancer stem cells (CSCs) are dominantly responsible for tumor progression and chemo/radio-resistance, resulting in tumor recurrence. 5-aminolevulinic acid (ALA) is metabolized to fluorescent protoporphyrin IX (PpIX) specifically in tumor cells, and therefore clinically used as a reagent for photodynamic diagnosis (PDD) and therapy (PDT) of cancers including gliomas. However, it remains to be clarified whether this method could be effective for CSC detection. Here, using flow cytometry-based analysis, we show that side population (SP)-defined C6 glioma CSCs (GSCs) displayed much less 5-ALA-derived PpIX fluorescence than non-GSCs...
February 7, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28160831/-glioblastoma-innovations-in-surgery
#11
Thiebaud Picart, Jacques Guyotat
One of the innovative principles in glioblastoma surgery consists in making the tumour fluorescent in order for it to be more easily visualised during the procedure. 5-aminolevulinic acid (5-ALA) undergoes an enzyme transformation, turning into another molecule, protoporphyrine IX (PPIX) whose property is fluorescence. It emits red light when it is stimulated by blue light.
February 2017: Revue de L'infirmière
https://www.readbyqxmd.com/read/28048774/su-f-j-215-non-thermal-pulsed-high-intensity-focused-ultrasound-therapy-combined-with-5-aminolevulinic-acid-an-in-vivo-pilot-study
#12
B Wang, W He, D Cvetkovic, L Chen, C Ma
PURPOSE: It has recently been shown that non-thermal pulsed high intensity focused ultrasound (pHIFU) has a cell-killing effect. The purpose of the study is to investigate the sonosensitizing effect of 5-Aminolevulinic Acid (5-ALA) in non-thermal pHIFU cancer therapy. METHODS: FaDu human head and neck squamous cell carcinoma cells were injected subcutaneously in the flanks of nude mice. After one to two weeks, the tumors reached the volume of 112 ± 8 mm3 and were assigned randomly into a non-thermal pHIFU group (n=9) and a non-thermal sonodynamic therapy (pHIFU after 5-ALA administration) group (n=7)...
June 2016: Medical Physics
https://www.readbyqxmd.com/read/28048178/su-f-t-674-in-vitro-study-of-5-aminolevulinic-acid-mediated-photo-dynamic-therapy-in-human-cancer-cell-lines
#13
D Cvetkovic, B Wang, R Gupta, C Ma
PURPOSE: Photodynamic therapy (PTD) is a promising cancer treatment modality. 5-sminolevulinic acid (ALA) is a clinically approved photosensitizer. Here we studied the effect of 5-ALA administration with irradiation on several cell lines in vitro. METHODS: Human head and neck (FaDu), lung (A549) and prostate (LNCaP) cancer cells (104/well) were seeded overnight in 96-well plates (Figure 1). 5-ALA at a range from 0.1 to 30.0mg/ml was added to confluent cells 3h before irradiation in 100ul of culture medium...
June 2016: Medical Physics
https://www.readbyqxmd.com/read/28038959/minispectrometer-with-handheld-probe-for-5-ala-based-fluorescence-guided-surgery-of-brain-tumors-preliminary-study-for-clinical-applications
#14
J F Cornelius, J M Placke, J Knipps, I Fischer, M Kamp, H J Steiger
INTRODUCTION: Recently a mini-spectrometer with a handheld probe quantifying 5-aminolevulinic acid (5-ALA) based fluorescence intensity of brain tumors was developped by Kim and co-workers to improve fluorescence-guided neurosurgery. OBJECTIVE: To evaluate if this new tool is capable to discriminate nuances of fluorescence intensity of strongly fluorescing tumors (glioblastomas (GBM) and meningiomas (MM)). To study different modes of measurement (touch/no-touch)...
December 27, 2016: Photodiagnosis and Photodynamic Therapy
https://www.readbyqxmd.com/read/28035368/5-aminolevulinic-acid-enhances-mitochondrial-stress-upon-ionizing-irradiation-exposure-and-increases-delayed-production-of-reactive-oxygen-species-and-cell-death-in-glioma-cells
#15
Kunihiro Ueta, Junkoh Yamamoto, Tohru Tanaka, Yoshiteru Nakano, Takehiro Kitagawa, Shigeru Nishizawa
5-Aminolevulinic acid (5-ALA) can accumulate protoporphyrin IX (PpIX) in tumour cell mitochondria and is well known for its utility in fluorescence-guided resection of malignant gliomas as a live molecular marker. Previously, we and other authors demonstrated that 5-ALA has a radiosensitizing effect for tumours. In the present study, we aimed to investigate the mechanism underlying the radiosensitizing effect of 5-ALA by focusing on glioma cell mitochondria. Using an enhancer (ciprofloxacin) of 5-ALA-induced PpIX accumulation, we evaluated the influence of ionizing irradiation (IR) and delayed reactive oxygen species (ROS) production 12 h after IR by colony-forming assay and flow cytometry (FCM) with different amounts of PpIX accumulation...
February 2017: International Journal of Molecular Medicine
https://www.readbyqxmd.com/read/28012197/interstitial-photodynamic-therapy-and-glioblastoma-light-fractionation-in-a-preclinical-model
#16
Henri-Arthur Leroy, Maximilien Vermandel, Anne-Sophie Vignion-Dewalle, Bertrand Leroux, Claude-Alain Maurage, Alain Duhamel, Serge Mordon, Nicolas Reyns
BACKGROUND: Glioblastoma is a high-grade cerebral tumor with local recurrence and poor outcome. Photodynamic therapy (PDT) is a localized treatment based on the light activation of a photosensitizer (PS) in the presence of oxygen, which results in the formation of cytotoxic species. The delivery of fractionated light may enhance treatment efficacy by reoxygenating tissues. OBJECTIVE: To evaluate the efficiency of two light-fractionation schemes using immunohistological data...
December 24, 2016: Lasers in Surgery and Medicine
https://www.readbyqxmd.com/read/28004205/vascular-alterations-after-photodynamic-therapy-mediated-by-5-aminolevulinic-acid-in-oral-leukoplakia
#17
Flávia Cristina Perillo Rosin, Ana Rita Ribeiro Barcessat, Giuliana Gadoni Giovanni Borges, Luciana Gonçalves Valente Ferreira, Luciana Corrêa
Impairment of vascular functions after photodynamic therapy (PDT) is frequently associated with tumor remission and is considered one of the main antineoplastic PDT effects. Vascular alterations in oral leukoplakia (OL) treated with PDT have not yet been described. The aim of this study was to evaluate the effect of topical 5-ALA-mediated PDT on the vascular network of 4NQO-induced OL in rats. After applying 4NQO topically on the tongue during 16 weeks, there was induction of dysplastic lesions, which were treated with two PDT sessions (with an interval of 72 h between them), using topical application of 5-ALA and posterior irradiation with a laser (90 J/cm(2) fluency)...
February 2017: Lasers in Medical Science
https://www.readbyqxmd.com/read/27984090/rip3-antagonizes-a-tsc2-mediated-pro-survival-pathway-in-glioblastoma-cell-death
#18
Gregory Fettweis, Emmanuel Di Valentin, Laurent L'homme, Cédric Lassence, Franck Dequiedt, Marianne Fillet, Isabelle Coupienne, Jacques Piette
Glioblastomas are the deadliest type of brain cancer and are frequently associated with poor prognosis and a high degree of recurrence despite removal by surgical resection and treatment by chemo- and radio-therapy. Photodynamic therapy (PDT) is a treatment well known to induce mainly necrotic and apoptotic cell death in solid tumors. 5-Aminolevulinic acid (5-ALA)-based PDT was recently shown to sensitize human glioblastoma cells (LN-18) to a RIP3 (Receptor Interacting Protein 3)-dependent cell death which is counter-acted by activation of autophagy...
October 27, 2016: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/27919664/photodynamic-therapy-with-green-light-for-the-treatment-of-vulvar-lichen-sclerosus-preliminary-results
#19
B J Osiecka, K Jurczyszyn, P Nockowski, M Murawski, P Ziółkowski
INTRODUCTION: The standard treatment for lichen sclerosus (LS) is symptomatic and is primarily based on the chronic use of corticosteroids, sometimes resulting in unsatisfactory effects. Therefore, other non-pharmacological methods are being sought, which are less aggravating for the patient. LS can be treated topically by using photodynamic therapy (PDT) based on 5-aminolevulinic acid (5-ALA). Unfortunately, therapy with the red light is often connected with severe local pain during the illumination...
December 2, 2016: Photodiagnosis and Photodynamic Therapy
https://www.readbyqxmd.com/read/27886396/5-ala-mediated-photodynamic-therapy-reduces-the-parasite-load-in-mice-infected-with-leishmania-braziliensis
#20
Danila Malheiros Souza, Polyanna Miranda Alves, Mona Lisa Fabiana Silva, Tony Paiva Paulino, Henrique Ovidio Coraspe, Mônica Miguel Sawan Mendonça, Betânia Maria Ribeiro, Marcos Vinicius da Silva, Virmondes Rodrigues Júnior, Denise Bertulucci Rocha Rodrigues
INTRODUCTION: Photodynamic therapy (PDT) has proven to be an effective alternative for the treatment of cutaneous leishmaniasis. Skin lesions consist of ulcers with well-defined raised edges, and granular floor. Th1 immune response is the protective profile in patients infected with Leishmania. In the present study, the photodynamic therapy with 5-aminolevulinic acid, the parasitic load, and the modulation of the immune response were evaluated in mice infected with Leishmania braziliensis...
November 25, 2016: Parasite Immunology
keyword
keyword
8236
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"