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5-ALA

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https://www.readbyqxmd.com/read/28582716/combination-of-active-targeting-enzyme-triggered-release-and-fluorescent-dye-into-gold-nanoclusters-for-endomicroscopy-guided-photothermal-photodynamic-therapy-to-pancreatic-ductal-adenocarcinoma
#1
Hui Li, Ping Wang, Yunxiang Deng, Meiying Zeng, Yan Tang, Wei-Hong Zhu, Yingsheng Cheng
Pancreatic ductal adenocarcinoma (PDAC) is one of the most devastating malignancies in patients, and there is an urgent need for an effective treatment method. Herein, we report a novel gold nanocluster-based platform for confocal laser endomicroscopy-guided photothermal therapy (PTT)/photodynamic therapy (PDT) for PDAC, which consists of four components: the PTT-carrier gold nanocluster, an active targeting ligand U11 peptide, a Cathepsin E (CTSE)-sensitive PDT therapy prodrug, and a CTSE-sensitive imaging agent (cyanine dye Cy5...
September 2017: Biomaterials
https://www.readbyqxmd.com/read/28578125/efficient-fluorescence-detection-of-protoporphyrin-ix-in-metastatic-lymph-nodes-of-murine-colorectal-cancer-stained-with-indigo-carmine
#2
Hisataka Matsuo, Yoshinori Harada, Takeo Minamikawa, Yoshiyuki Kato, Yasutoshi Murayama, Eigo Otsuji, Tetsuro Takamatsu, Hideo Tanaka
Protoporphyrin IX (PpIX), a biochemical converted from 5-aminolevulinc acid (5-ALA) in living cells, is useful for intraoperative fluorescent detection of cancer metastasis in lymph nodes (LNs). However, unknown is whether the fluorescence of PpIX can be detected in the LNs when they coexist with indigo carmine, a blue dye commonly used for identification of sentinel LNs during surgery. To address this issue, we sought to evaluate the diagnostic usefulness of PpIX fluorescence in the presence of indigo carmine in a mouse LN metastasis model of rectal cancer after administration of 5-ALA...
May 31, 2017: Photodiagnosis and Photodynamic Therapy
https://www.readbyqxmd.com/read/28566408/5-ala-ameliorates-hepatic-steatosis-through-ampk-signaling-pathway
#3
Haoyong Yu, Mingliang Zhang, Yunqin Ma, Junxi Lu, Pan Pan, Haibing Chen, Weiping Jia
5-Aminolevulinic acid (5-ALA), the first compound in the porphyrin synthesis pathway, has been reported to ameliorate the diabetic state in Otsuka Long-Evans Tokushima Fatty rats by reducing fat pad weight in the retroperitoneal region. Dietary supplementation with 5-ALA has additionally demonstrated the capacity to lower blood glucose and HbA1c levels among subjects with diabetes. The etiology of Nonalcoholic Fatty Liver Disease (NAFLD) is complex and its typical characteristics include obesity and insulin resistance...
May 31, 2017: Journal of Molecular Endocrinology
https://www.readbyqxmd.com/read/28551838/5-ala-fluorescence-in-intraparenchymal-endodermal-cysts
#4
José Pedro Lavrador, Christian Brogna, Francesco Vergani, Fay Greenway, Miren Aizpurua, Ranj Bhangoo
In recent years, new indications have been suggested for 5-ALA, particularly for cystic lesions. We report the use of 5-ALA fluorescence in an intraparenchymal supratentorial endodermal cyst of a 52-year-old female presenting with headache, progressive right side hemiparesis and anomic aphasia. She underwent an image-guided 5-ALA-assisted left minicraniotomy for fenestration of the cystic lesion into the ventricular system. The capsule of the cyst was noted to fluoresce with 5-ALA. She recovered from the previous deficits and the cyst decreased in size...
May 27, 2017: Acta Neurochirurgica
https://www.readbyqxmd.com/read/28536938/enhancement-of-5-aminolevulinic-acid-production-by-metabolic-engineering-of-the-glycine-biosynthesis-pathway-in-corynebacterium-glutamicum
#5
Yalan Zou, Tao Chen, Lili Feng, Shuanghong Zhang, Dongxu Xing, Zhiwen Wang
OBJECTIVE: To construct a strain of Corynebacterium glutamicum capable of efficiently producing 5-aminolevulinic acid (5-ALA) via the C4 pathway by modification of serine and glycine pathway using glucose as sole carbon source. RESULTS: The recombinant C. glutamicum strain AP2 harboring a codon-optimized hemA gene from Rhodobacter sphaeroides was used as host strain for 5-ALA production. A plasmid harboring the serine operon, which contained serB, serC and the site-specific mutant serA (Δ197) , was constructed and introduced into C...
May 23, 2017: Biotechnology Letters
https://www.readbyqxmd.com/read/28528422/5-ala-fluorescence-guided-endoscopic-surgery-for-mixed-germ-cell-tumors
#6
Junichi Takeda, Masahiro Nonaka, Yi Li, Yumiko Komori, Takamasa Kamei, Ryoichi Iwata, Tetsuo Hashiba, Kunikazu Yoshimura, Akio Asai
5-Aminolevulinic acid (5-ALA) fluorescence-guided surgery is widely used for detection and planning of resection of malignant gliomas and other brain tumors. However, no reports have described 5-ALA fluorescence-guided surgery or direct visualization of germ cell tumors. Here, we report two cases of germ cell tumors in which a positive 5-ALA fluorescent signal was visualized with a neuroendoscope. Both cases had a tumor in the pineal region that was associated with hydrocephalus. The patients underwent surgery after administration of 5-ALA...
May 20, 2017: Journal of Neuro-oncology
https://www.readbyqxmd.com/read/28522184/is-fluorescence-guided-surgery-with-5-ala-in-eloquent-areas-for-malignant-gliomas-a-reasonable-and-useful-technique
#7
T Picart, X Armoiry, J Berthiller, C Dumot, I Pelissou-Guyotat, F Signorelli, J Guyotat
INTRODUCTION: High-grade gliomas surgery in eloquent areas must achieve two pivotal aims: oncological efficacy and preservation of unimpaired neurological functions or improvement of impaired neurological functions. Here, we evaluated the safety and the usefulness of 5-ALA fluorescence-guided surgery in eloquent areas. MATERIAL AND METHODS: Single center, retrospective and consecutive series of adult patients operated on for a supratentorial glioblastoma between November 2012 and November 2015...
May 15, 2017: Neuro-Chirurgie
https://www.readbyqxmd.com/read/28500562/epithelial-growth-factor-receptor-expression-influences-5-ala-induced-glioblastoma-fluorescence
#8
Andrea O Fontana, Deborah Piffaretti, Francesco Marchi, Floriana Burgio, Ana Bela Faia-Torres, Paolo Paganetti, Sandra Pinton, Uwe Pieles, Michael Reinert
The extent of 5-aminolevulinic acid (5-ALA) guided tumor resection has a determining impact in high-grade glioma and glioblastoma surgery. Yet the intensity of the 5-ALA induced fluorescence may vary within the tumor. We aimed to correlate 5-ALA induced fluorescence with the expression of epithelial growth factor receptor (EGFR) and its constitutively active version EGFRvIII in different glioblastoma (GBM) cell lines. To elucidate the role of EGFR in the metabolism of 5-ALA in GBM cell lines with variable EGFR expression status, we analyzed the activation of EGFR by its primary ligand EGF, and its downstream effect on Heme oxygenase-1 (HO-1), a key enzyme regulating the metabolism of Protoporphyrin IX (PpIX), the fluorescent metabolite of 5-ALA...
May 12, 2017: Journal of Neuro-oncology
https://www.readbyqxmd.com/read/28498936/a-phase-1-dose-escalation-study-of-oral-5-aminolevulinic-acid-in-adult-patients-undergoing-resection-of-a-newly-diagnosed-or-recurrent-high-grade-glioma
#9
Jeffrey W Cozzens, Barbara C Lokaitis, Brian E Moore, Devin V Amin, José A Espinosa, Margaret MacGregor, Alex P Michael, Breck A Jones
BACKGROUND: The utility of oral 5-aminolevulinic acid (5-ALA)/protoporphyrin fluorescence for the resection of high-grade gliomas is well documented. This drug has received regulatory approval in Europe but awaits approval in the United States. OBJECTIVE: To identify the appropriate dose and toxicity or harms of 5-ALA used for enhanced intraoperative visualization of malignant brain tumors, reported from a single medical center in the United States. METHODS: Prior to craniotomy for resection of a presumed high-grade glioma, individuals were given oral 5-ALA as part of a rapid dose-escalation scheme...
May 10, 2017: Neurosurgery
https://www.readbyqxmd.com/read/28495088/fluorescence-guided-resection-with-5-aminolevulinic-acid-of-a-pilomyxoid-astrocytoma-of-the-third-ventricle
#10
Luis Miguel Bernal García, Jose Manuel Cabezudo Artero, Rafael García Moreno, Maria Bella Marcelo Zamorano, Carlos Mayoral Guisado
Fluorescence-guided resection with 5-aminolevulinic acid has been shown to be useful in the resection of certain brain tumors other than high grade gliomas, facilitating the intraoperative differentiation of neoplastic tissue. The technique enables the surgeon to ensure that no tumor fragments remain, thereby achieving higher rates of complete resection. Tihan first described pilomyxoid astrocytomas in 1999. They are currently classified as grade II astrocytoma according to the WHO classification system and, because of their tendency to recur and their dissemination through the cerebrospinal fluid pathways, they are considered to be more aggressive than pilocytic astrocytoma...
May 8, 2017: Neurocirugía
https://www.readbyqxmd.com/read/28472723/activity-of-phosphatase-sensitive-5-aminolevulinic-acid-prodrugs-in-cancer-cell-lines
#11
Viktorija Herceg, Norbert Lange, Eric Allémann, Andrej Babič
5-aminolevulinc acid (5-ALA)-based photodynamic therapy (PDT) and photodiagnosis (PD) present many advantages over treatments with conventional photosensitizers (PS). It offers great tumor specificity, reduced photosensitivity reactions caused by PS accumulation in non-targeted tissues and also inherent PS metabolism into endogenous non-fluorescent heme. However, chemical instability, low bioavailability and poor pharmacokinetic profile limit systemic efficacy of 5-ALA. Here, we present a comprehensive in vitro evaluation of novel phosphatase-sensitive prodrugs of 5-ALA...
June 2017: Journal of Photochemistry and Photobiology. B, Biology
https://www.readbyqxmd.com/read/28454245/improving-contrast-enhancement-in-magnetic-resonance-imaging-using-5-aminolevulinic-acid-induced-protoporphyrin-ix-for-high-grade-gliomas
#12
Junkoh Yamamoto, Shingo Kakeda, Tetsuya Yoneda, Shun-Ichiro Ogura, Shohei Shimajiri, Tohru Tanaka, Yukunori Korogi, Shigeru Nishizawa
Magnetic resonance imaging (MRI) with a gadolinium-based contrast agent is the gold standard for high-grade gliomas (HGGs). The compound 5-aminolevulinic acid (5-ALA) undergoes a high rate of cellular uptake, particularly in cancer cells. In addition, fluorescence-guided resection with 5-ALA is widely used for imaging HGGs. 5-ALA is water soluble, while protoporphyrin IX (PpIX) is water insoluble. It was speculated whether converting from 5-ALA to PpIX may relatively increase intracellular water content, and consequently, might enhance the T2 signal intensity in HGG...
March 2017: Oncology Letters
https://www.readbyqxmd.com/read/28432590/pathological-analysis-of-the-surgical-margins-of-resected-glioblastomas-excised-using-photodynamic-visualization-with-both-5-aminolevulinic-acid-and-fluorescein-sodium
#13
Hirohito Yano, Noriyuki Nakayama, Naoyuki Ohe, Kazuhiro Miwa, Jun Shinoda, Toru Iwama
During glioma resection, 5-aminolevulinic acid (5-ALA) and fluorescein sodium (Fl-Na) are used for photodynamic tumor visualization. The objective of this study was to evaluate the pathological findings of the boundary zone between the tumor and adjacent normal brain in glioblastoma patients undergoing simultaneous double staining with 5-ALA and Fl-Na during surgery. Eight patients received 5-ALA (20 mg/kg orally) before the induction of general anesthesia, and Fl-Na (20 mg/kg) was administered intravenously before the dural incision was performed...
April 21, 2017: Journal of Neuro-oncology
https://www.readbyqxmd.com/read/28397137/fluorescence-guided-resection-of-extracranial-soft-tissue-tumour-infiltration-in-atypical-meningioma
#14
Florian Scheichel, Karl Ungersboeck, Melitta Kitzwoegerer, Franz Marhold
The exact role of 5-aminolevulinic acid (5-ALA) fluorescence-guided meningioma resection is as yet unclear. Although most of the meningiomas show positive fluorescence, the influence on outcome, fluorescence heterogeneity within the tumour, the correlation between fluorescence and the proliferation rate, as well as different quantitative measurements are a matter of debate. Positive fluorescence in bone infiltrative meningiomas is well described, whereas little data exist about 5-ALA fluorescence within soft tissue infiltration...
June 2017: Acta Neurochirurgica
https://www.readbyqxmd.com/read/28380684/effectiveness-of-photodynamic-therapy-in-elimination-of-hpv-16-and-hpv-18-associated-with-cin-i-in-mexican-women
#15
Elizabeth Maldonado Alvarado, M Olivia Osorio Peralta, Alejandra Moreno Vázquez, L Alejandra Martínez Guzmán, M Eugenia Melo Petrone, Z Iveth Enriquez Mar, D Estela Jovel Galdamez, Bárbara Carrión Solana, Guadalupe Balderas Martínez, Eduarda Parra, R Inés Castellanos Oliveros, R Linda Bello Leiva, Araceli Espinosa Montesinos, Citlalli Barrera Mendoza, S Eugenia Medina García, Eva Ramón Gallegos
The present study aimed to determine the effectiveness of photodynamic therapy (PDT), using δ-aminolevulinic acid (5-ALA), in the elimination of premalignant cervical lesions in Mexican patients with human papillomavirus (HPV) infection and/or cervical intraepithelial neoplasia (CIN). Thirty women diagnosed with CIN I and/or positive for HPV participated in the study. Topical 6% 5-ALA in gel form was applied to the uterine cervix; after 4 h, the lesion area was irradiated with a light dose of 200 J/cm(2) at 635 nm...
April 5, 2017: Photochemistry and Photobiology
https://www.readbyqxmd.com/read/28379547/randomized-prospective-double-blinded-study-comparing-3-different-doses-of-5-aminolevulinic-acid-for-fluorescence-guided-resections-of-malignant-gliomas
#16
Walter Stummer, Herbert Stepp, Otmar D Wiestler, Uwe Pichlmeier
BACKGROUND: Five-aminolevulinic acid (5-ALA) is used for fluorescence-guided resections of malignant glioma at a dose of 20 mg/kg; yet, it is unknown whether lower doses may also provide efficacy. OBJECTIVE: To perform a double-blinded randomized study comparing 3 different doses of 5-ALA. METHODS: Twenty-one patients with suspected malignant glioma were randomly assigned to 0.2, 2, or 20 mg/kg 5-ALA. Investigators were unaware of dose. Intraoperatively, regions of interest were first defined in tumor core, margin, and adjacent white matter under white light...
April 1, 2017: Neurosurgery
https://www.readbyqxmd.com/read/28346389/artesunate-enhances-the-cytotoxicity-of-5-aminolevulinic-acid-based-sonodynamic-therapy-against-mouse-mammary-tumor-cells-in-vitro
#17
Tomohiro Osaki, Yoshihiro Uto, Masahiro Ishizuka, Tohru Tanaka, Nobuyasu Yamanaka, Tsukasa Kurahashi, Kazuo Azuma, Yusuke Murahata, Takeshi Tsuka, Norihiko Itoh, Tomohiro Imagawa, Yoshiharu Okamoto
Sonodynamic therapy (SDT) kills tumor cells through the synergistic effects of ultrasound (US) and a sonosensitizer agent. 5-Aminolevulinic acid (5-ALA) has been used as a sonodynamic sensitizer for cancer treatment. However, studies have shown that 5-ALA-based SDT has limited efficacy against malignant tumors. In this study, we examined whether artesunate (ART) could enhance the cytotoxicity of 5-ALA-based SDT against mouse mammary tumor (EMT-6) cells in vitro. In the ART, ART + US, ART + 5-ALA, and ART + 5-ALA + US groups, the cell survival rate correlated with ART concentration, and decreased with increasing concentrations of ART...
March 27, 2017: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/28338432/dual-labeling-with-5-aminolevulinic-acid-and-fluorescein-for-fluorescence-guided-resection-of-high-grade-gliomas-technical-note
#18
Eric Suero Molina, Johannes Wölfer, Christian Ewelt, André Ehrhardt, Benjamin Brokinkel, Walter Stummer
OBJECTIVE Fluorescence guidance with 5-aminolevulinic acid (5-ALA) helps improve resections of malignant gliomas. However, one limitation is the low intensity of blue light for background illumination. Fluorescein has recently been reintroduced into neurosurgery, and novel microscope systems are available for visualizing this fluorochrome, which highlights all perfused tissues but has limited selectivity for tumor detection. Here, the authors investigate a combination of both fluorochromes: 5-ALA for distinguishing tumor and fluorescein for providing tissue fluorescence of adjacent brain tissue...
March 24, 2017: Journal of Neurosurgery
https://www.readbyqxmd.com/read/28338177/5-aminolevulinic-acid-photodynamic-therapy-stimulates-local-immunity-in-patients-with-condylomata-acuminata-via-activation-of-t-lymphocytes
#19
J Du, Q Cheng, Z Zhang, J-F Wu, F Li, S-Y Chen, Y-L Wang, X-N Lu, J-H Xu
OBJECTIVE: 5-Aminolevulinic acid photodynamic therapy (5-ALA-PDT) for condylomata acuminate (CA) is effective and safe, but how this treatment affects immune reaction is poorly understood. We aimed to explore the effects of PDT on local immunity in CA. PATIENTS AND METHODS: Immunohistochemical analysis before and after PDT was performed to analyze local changes in the distribution of T lymphocytes, CD123+ plasmacytoid dendritic cells (pDCs) and CD1a+ myeloid DCs...
March 2017: European Review for Medical and Pharmacological Sciences
https://www.readbyqxmd.com/read/28321653/5-aminolevulinic-acid-modulates-antioxidant-defense-systems-and-mitigates-drought-induced-damage-in-kentucky-bluegrass-seedlings
#20
Kuiju Niu, Xiang Ma, Guoling Liang, Huiling Ma, Zhifeng Jia, Wenhui Liu, Qianqian Yu
Drought stress occurs frequently and severely as a result of global climate change, and it exerts serious effects on plants. 5-Aminolevulinic acid (5-ALA) plays a crucial role in conferring abiotic stress tolerance in plants. To enhance the drought tolerance of turfgrass and investigate the effects of 5-ALA on antioxidant metabolism and gene expression under drought stress conditions, exogenous 5-ALA was applied by foliar spraying before Kentucky bluegrass (Poa pratensis L.) seedlings were exposed to drought [induced by 10% polyethylene glycol (PEG)] stress for 20 days...
March 20, 2017: Protoplasma
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