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https://www.readbyqxmd.com/read/29341902/curcumin-potentiates-the-function-of-human-%C3%AE-7-nicotinic-acetylcholine-receptors-expressed-in-sh-ep1-cells
#1
Eslam El Nebrisi, Lina T Al Kury, Keun-Hang Susan Yang, Petrilla Jayaprakash, Frank C Howarth, Nadine Kabbani, Murat Oz
Effects of curcumin, a biologically active ingredient of turmeric, were tested on the Ca2+ transients induced by the activation of α7 subunit of the human nicotinic acetylcholine (α7 nACh) receptor expressed in SH-EP1 cells. Curcumin caused a significant potentiation of choline (1 mM)-induced Ca2+ transients with an EC50 value of 133 nM. The potentiating effect of curcumin was not observed in Ca2+ transients induced by high K+ (60 mM) containing solutions or activation of α4β2 nACh receptors and the extent of curcumin potentiation was not altered in the presence of Ca2+ channel antagonists nifedipine (1 μM), verapamil (1 μM), ω-conotoxin (1 μM), and bepridil (10 μM)...
January 13, 2018: Neurochemistry International
https://www.readbyqxmd.com/read/29341613/what-gives-an-insulin-hexamer-its-unique-shape-and-stability-role-of-ten-confined-water-molecules
#2
Saumyak Mukherjee, Sayantan Mondal, Ashish Anilrao Deshmukh, Balasubramanian Gopal, Biman Bagchi
Self-assembly of proteins often gives rise to interesting quasi-stable structures that serve important biological purposes. Insulin hexamer is such an assembly. While monomer is the biologically active form of insulin, hexamer serves as the storehouse of the hormone. The hexamer also prevents the formation of higher order aggregates. While several studies explored the role of bivalent metal ions like Zn2+, Ca2+ etc. in the stabilization of the hexameric form, the role of water molecules has been ignored. We combine molecular dynamics simulations, quantum calculations and X-ray analyses to discover that a team of approximately ten water molecules confined inside a barrel-shaped nano-cavity at the center of insulin hexamer is one of the major causes that account for the unusual stability of the biomolecular assembly...
January 17, 2018: Journal of Physical Chemistry. B
https://www.readbyqxmd.com/read/29341416/resveratrol-influences-pancreatic-islets-by-opposing-effects-on-electrical-activity-and-insulin-release
#3
Simone Brouwer, Theresa Hoffmeister, Anne Gresch, Lisa Schönhoff, Martina Düfer
SCOPE: Resveratrol is suggested to improve glycemic control by activation of sirtuin 1 (SIRT1) and has already been tested clinically. Our investigation characterizes the targets of resveratrol in pancreatic beta cells and their contribution to short- and long-term effects on insulin secretion. METHODS AND RESULTS: Islets or beta cells were isolated from C57Bl/6N mice. Electrophysiology was performed with microelectrode arrays and patch-clamp technique, insulin secretion and content were determined by radioimmunoassay, cAMP was measured by enzyme-linked immunosorbent assay and cytosolic Ca2+ concentration by fluorescence methods...
January 17, 2018: Molecular Nutrition & Food Research
https://www.readbyqxmd.com/read/29341317/apoptosis-induction-in-k562-human-myelogenous-leukaemia-cells-is-connected-to-the-modulation-of-wnt-%C3%AE-catenin-signalling-by-bhx-a-novel-pyrazoline-derivative
#4
Hanmei Bao, Qing Zhang, Yibo Du, Cai Zhang, Hui Xu, Zhongling Zhu, Zhao Yan
OBJECTIVES: The goal of this study was to explore the effects of BHX on human chronic myeloid leukaemia (CML) cells and to elucidate the underlying molecular mechanism. MATERIALS AND METHODS: CML cell line K562 cells were treated with BHX. The effects of BHX on cell proliferation, apoptosis and cell cycle were detected. Subsequently, the caspase, ATP activity, Ca2+ , ROS and mitochondrial membrane potential (MMP) levels treated with various concentrations of BHX were analysed...
January 16, 2018: Cell Proliferation
https://www.readbyqxmd.com/read/29340119/mef2-signaling-and-human-diseases
#5
REVIEW
Xiao Chen, Bing Gao, Murugavel Ponnusamy, Zhijuan Lin, Jia Liu
The members of myocyte Enhancer Factor 2 (MEF2) protein family was previously believed to function in the development of heart and muscle. Recent reports indicate that they are also closely associated with development and progression of many human diseases. Although their role in cancer biology is well established, the molecular mechanisms underlying their action is yet largely unknown. MEF2 family is closely associated with various signaling pathways, including Ca2+ signaling, MAP kinase signaling, Wnt signaling, PI3K/Akt signaling, etc...
December 19, 2017: Oncotarget
https://www.readbyqxmd.com/read/29340082/reciprocal-sensitivity-of-diffuse-large-b-cell-lymphoma-cells-to-bcl-2-inhibitors-bird-2-versus-venetoclax
#6
Tamara Vervloessem, Haidar Akl, Thomas Tousseyn, Humbert De Smedt, Jan B Parys, Geert Bultynck
Bcl-2 is often upregulated in cancers to neutralize the BH3-only protein Bim at the mitochondria. BH3 mimetics (e.g. ABT-199 (venetoclax)) kill cancer cells by targeting Bcl-2's hydrophobic cleft and disrupting Bcl-2/Bim complexes. Some cancers with elevated Bcl-2 display poor responses towards BH3 mimetics, suggesting an additional function for anti-apoptotic Bcl-2 in these cancers. Indeed, Bcl-2 via its BH4 domain prevents cytotoxic Ca2+ release from the endoplasmic reticulum (ER) by directly inhibiting the inositol 1,4,5-trisphosphate receptor (IP3R)...
December 19, 2017: Oncotarget
https://www.readbyqxmd.com/read/29339804/ephb6-and-testosterone-in-concert-regulate-epinephrine-release-by-adrenal-gland-chromaffin-cells
#7
Yujia Wang, Wei Shi, Alexandre Blanchette, Junzheng Peng, Shijie Qi, Hongyu Luo, Jonathan Ledoux, Jiangping Wu
Erythropoietin-producing human hepatocellular receptor (EPH) B6 (EPHB6) is a member of the receptor tyrosine kinase family. We previously demonstrated that EPHB6 knockout reduces catecholamine secretion in male but not female mice, and castration reverses this phenotype. We showed here that male EPHB6 knockout adrenal gland chromaffin cells presented reduced acetylcholine-triggered Ca2+ influx. Such reduction depended on the non-genomic effect of testosterone. Increased large conductance calcium-activated potassium channel current densities were recorded in adrenal gland chromaffin cells from male EPHB6 knockout mice but not from castrated knockout or female knockout mice...
January 16, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29339801/cardiac-glycoside-aglycones-inhibit-hiv-1-gene-expression-by-a-mechanism-requiring-mek1-2-erk1-2-signaling
#8
Raymond W Wong, Clifford A Lingwood, Mario A Ostrowski, Tyler Cabral, Alan Cochrane
The capacity of HIV-1 to develop resistance to current drugs calls for innovative strategies to control this infection. We aimed at developing novel inhibitors of HIV-1 replication by targeting viral RNA processing-a stage dependent on conserved host processes. We previously reported that digoxin is a potent inhibitor of this stage. Herein, we identify 12 other cardiac glycoside/aglycones or cardiotonic steroids (CSs) that impede HIV growth in HIV-infected T cells from clinical patients at IC50s (1.1-1.3 nM) that are 2-26 times below concentrations used in patients with heart conditions...
January 16, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29339732/glyburide-and-retinoic-acid-synergize-to-promote-wound-healing-by-anti-inflammation-and-rip140-degradation
#9
Yi-Wei Lin, Pu-Ste Liu, Kasey Ah Pook, Li-Na Wei
Chronic inflammation underlies the development of metabolic diseases and individuals with metabolic disease often also suffer from delayed wound healing due to prolonged inflammation. Resolving inflammation provides a therapeutic strategy in treating metabolic diseases. We previously showed that during an anti-inflammatory response when macrophages were alternatively (M2) polarized, retinoic acid (RA) dramatically activated arginase 1 gene (Arg1), a gene crucial for wound healing. Here we report that a widely used sulfonylurea drug for type 2 diabetes mellitus (T2DM), glyburide, enhances the anti-inflammatory response and synergizes with RA to promote wound healing...
January 16, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29339696/chronic-ethanol-increases-calcium-calmodulin-dependent-protein-kinaseii%C3%AE-gene-expression-and-decreases-monoamine-oxidase-amount-in-rat-heart-muscles-rescue-effect-of-zingiber-officinale-ginger-extract
#10
Elaheh Heshmati, Alireza Shirpoor, Fatemeh Kheradmand, Mohammad Alizadeh, Farzaneh Hosseini Gharalari
OBJECTIVE: Association between chronic alcohol intake and cardiac abnormality is well known; however, the precise underlying molecular mediators involved in ethanol-induced heart abnormalities remain elusive. This study investigated the effect of chronic ethanol exposure on calcium/calmodulin-dependent protein kinase IIδ (CaMKIIδ) gene expression and monoamine oxidase (MAO) levels and histological changes in rat heart. It was also planned to find out whether Zingiber officinale (ginger) extract mitigated the abnormalities induced by ethanol in rat heart...
January 2018: Anatolian Journal of Cardiology
https://www.readbyqxmd.com/read/29339608/pilot-study-of-oilfield-wastewater-treatment-by-micro-flocculation-filtration-process
#11
Shaoxiong Si, Zhong Yan, Zhaobo Gong, Pengfei Liu, Yumin Zhang, Yu Xiang
In order to meet the latest Environmental Protection Law of China on wastewater discharge standards, this paper studied a pilot-scale micro-flocculation filtration pretreatment process for the treatment of oilfield wastewater. The experiment showed that the removal rate of oil and suspended solids (SS) respectively increased from 91.52% to 95.38% and from 66.42% to 97.19%. After the treatment by the micro-flocculation filtration device, the relevant characteristics of the discharge wastewater satisfied the latest standards continuously...
January 2018: Water Science and Technology: a Journal of the International Association on Water Pollution Research
https://www.readbyqxmd.com/read/29339535/functional-changes-of-ampa-responses-in-human-induced-pluripotent-stem-cell-derived-neural-progenitors-in-fragile-x-syndrome
#12
Venkat Swaroop Achuta, Tommi Möykkynen, Ulla-Kaisa Peteri, Giorgio Turconi, Claudio Rivera, Kari Keinänen, Maija L Castrén
Altered neuronal network formation and function involving dysregulated excitatory and inhibitory circuits are associated with fragile X syndrome (FXS). We examined functional maturation of the excitatory transmission system in FXS by investigating the response of FXS patient-derived neural progenitor cells to the glutamate analog (AMPA). Neural progenitors derived from induced pluripotent stem cell (iPSC) lines generated from boys with FXS had augmented intracellular Ca2+ responses to AMPA and kainate that were mediated by Ca2+-permeable AMPA receptors (CP-AMPARs) lacking the GluA2 subunit...
January 16, 2018: Science Signaling
https://www.readbyqxmd.com/read/29339457/curcumin-acts-as-a-positive-allosteric-modulator-of-%C3%AE-7-nicotinic-acetylcholine-receptors-and-reverses-nociception-in-mouse-models-of-inflammatory-pain
#13
Eslam Gaber El Nebrisi, Deniz Bagdas, Wisam Toma, Halima Al Samri, Anna Brodzik, Yasmin Alkhlaif, Keun-Hang Susan Yang, Frank C Howarth, Imad M Damaj, Murat Oz
Effects of curcumin, a major ingredient of turmeric, were tested on the function of α7 subunit of the human nicotinic acetylcholine (α7 nACh) receptor expressed in Xenopus oocytes and on nociception in mouse models of tonic and visceral pain. Curcumin caused a significant potentiation of ACh (100 μM)-induced currents with an EC50 value of 0.2 µM. The effect of curcumin was not dependent on the activation of G-proteins and protein kinases and did not involve Ca2+-dependent Cl- channels expressed endogenously in oocytes...
January 16, 2018: Journal of Pharmacology and Experimental Therapeutics
https://www.readbyqxmd.com/read/29339227/a-novel-biphenolic-ligand-for-selective-mg2-and-zn2-ions-sensing-followed-by-colorimetric-spectroscopic-and-cell-imaging-methods
#14
Palanisamy Uma Maheswari, Duraisamy Renuga, H L Jeeva Kumari, Kandasamy Ruckmani
The (E)-2-((2-hydrohy-5-methylphenylimino) methyl) phenol ligand was synthesized. The receptor was characterized by IR, 1H and 13C NMR and CHN analysis. The ligand exhibits colorimetric and fluorometric sensing of Zn2+ and Mg2+ ions in semi-aqueous medium (DMSO-H2O). The receptor was tested with series of transition metal ions (Cr2+, Fe2+, Ni2+, Co2+, Cu2+, Zn2+) and heavy metal ions (Sn2+, Pd2+, Ce2+, Hg2+, Cd2+) and the essential human body elements like Ca2+, Mg2+, Na+ and K+ ions. The naked eye colorimetric sensing was absorbed only for Zn2+ and Mg2+...
January 12, 2018: European Journal of Pharmaceutical Sciences
https://www.readbyqxmd.com/read/29339150/altered-function-of-neuronal-l-type-calcium-channels-in-ageing-and-neuroinflammation-implications-in-age-related-synaptic-dysfunction-and-cognitive-decline
#15
REVIEW
Sheeja Navakkode, Chao Liu, Tuck Wah Soong
The rapid developments in science have led to an increase in human life expectancy and thus, ageing and age-related disorders/diseases have become one of the greatest concerns in the 21st century. Cognitive abilities tend to decline as we get older. This age-related cognitive decline is mainly attributed to aberrant changes in synaptic plasticity and neuronal connections. Recent studies show that alterations in Ca2+ homeostasis underlie the increased vulnerability of neurons to age-related processes like cognitive decline and synaptic dysfunctions...
January 12, 2018: Ageing Research Reviews
https://www.readbyqxmd.com/read/29339138/hyperglycaemia-disrupts-conducted-vasodilation-in-the-resistance-vasculature-of-db-db-mice
#16
Hamish A L Lemmey, Xi Ye, Hong Ding, Chris R Triggle, Christopher J Garland, Kim A Dora
Vascular dysfunction in small resistance arteries is observed during chronic elevations in blood glucose. Hyperglycaemia-associated effects on endothelium-dependent vasodilation have been well characterized, but effects on conducted vasodilation in the resistance vasculature are not known. Small mesenteric arteries were isolated from healthy and diabetic db/db mice, which were used as a model of chronic hyperglycaemia. Endothelium-dependent vasodilation via the Gq/11-coupled proteinase activated receptor 2 (PAR2) was stimulated with the selective agonist SLIGRL...
January 12, 2018: Vascular Pharmacology
https://www.readbyqxmd.com/read/29338710/a-genetically-encoded-ca2-indicator-based-on-circularly-permutated-sea-anemone-red-fluorescent-protein-eqfp578
#17
Yi Shen, Hod Dana, Ahmed S Abdelfattah, Ronak Patel, Jamien Shea, Rosana S Molina, Bijal Rawal, Vladimir Rancic, Yu-Fen Chang, Lanshi Wu, Yingche Chen, Yong Qian, Matthew D Wiens, Nathan Hambleton, Klaus Ballanyi, Thomas E Hughes, Mikhail Drobizhev, Douglas S Kim, Minoru Koyama, Eric R Schreiter, Robert E Campbell
BACKGROUND: Genetically encoded calcium ion (Ca2+) indicators (GECIs) are indispensable tools for measuring Ca2+ dynamics and neuronal activities in vitro and in vivo. Red fluorescent protein (RFP)-based GECIs have inherent advantages relative to green fluorescent protein-based GECIs due to the longer wavelength light used for excitation. Longer wavelength light is associated with decreased phototoxicity and deeper penetration through tissue. Red GECI can also enable multicolor visualization with blue- or cyan-excitable fluorophores...
January 16, 2018: BMC Biology
https://www.readbyqxmd.com/read/29338006/a-model-for-cooperative-gating-of-l-type-ca2-channels-and-its-effects-on-cardiac-alternans-dynamics
#18
Daisuke Sato, Rose E Dixon, Luis F Santana, Manuel F Navedo
In ventricular myocytes, membrane depolarization during the action potential (AP) causes synchronous activation of multiple L-type CaV1.2 channels (LTCCs), which trigger the release of calcium (Ca2+) from the sarcoplasmic reticulum (SR). This results in an increase in intracellular Ca2+ (Cai) that initiates contraction. During pulsus alternans, cardiac contraction is unstable, going from weak to strong in successive beats despite a constant heart rate. These cardiac alternans can be caused by the instability of membrane potential (Vm) due to steep AP duration (APD) restitution (Vm-driven alternans), instability of Cai cycling (Ca2+-driven alternans), or both, and may be modulated by functional coupling between clustered CaV1...
January 16, 2018: PLoS Computational Biology
https://www.readbyqxmd.com/read/29337693/in-vitro-reconstitution-and-biochemical-characterization-of-human-phospholipid-scramblase-3-phospholipid-specificity-and-metal-ion-binding-studies
#19
Santosh Kumar Palanirajan, Ulaganathan Sivagnanam, Sowmiya Murugan, Sathyanarayana N Gummadi
Human phospholipid scramblase 3 (hPLSCR3) is a single pass transmembrane protein that plays a vital role in fat metabolism, mitochondrial function, structure, maintenance and apoptosis. The mechanism of action of scramblases remains still unknown and the role of scramblases in phospholipid translocation is heavily debated. hPLSCR3 is the only member of scramblase family localized to mitochondria and is involved in cardiolipin translocation at the mitochondrial membrane. Direct biochemical evidence of phospholipid translocation by hPLSCR3 is yet to be reported...
June 27, 2017: Biological Chemistry
https://www.readbyqxmd.com/read/29337674/a-study-on-blocking-store-operated-ca2-entry-in-pulmonary-arterial-smooth-muscle-cells-with-xyloketals-from-marine-fungi
#20
Jie-Bin Zhou, Ying-Ying Sun, Ying-Lin Zheng, Chu-Qin Yu, Hua-Qing Lin, Ji-Yan Pang
In this study, the effect of four xyloketals 1-4 on store-operated calcium entry (SOCE) was investigated in primary distal pulmonary arterial smooth muscle cells (PASMCs) isolated from mice. The results showed that xyloketal A (1), an unusual ketal with C-3 symmetry, exhibited strong SOCE blocking activity. Secretion of interleukin-8 (IL-8) was also inhibited by xyloketal A. The parallel artificial membrane permeability assay (PAMPA) of 1-4 suggested that these xyloketals penetrated easily through the cell membrane...
December 20, 2017: Acta Pharmaceutica
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