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https://www.readbyqxmd.com/read/29786742/a-novel-alendronate-functionalized-nanoprobe-for-simple-colorimetric-detection-of-cancer-associated-hypercalcemia
#1
Abhishek Sahu, Youngmin Hwang, Cristian Vilos, Jong-Min Lim, Sunghyun Kim, Won Il Choi, Giyoong Tae
The calcium (Ca2+) ion concentration in the blood serum is tightly regulated, and any abnormalities in the level of serum calcium ions are associated with many potentially dangerous diseases. Thus, monitoring of the Ca2+ ion concentration in the blood serum is of fundamental importance. Gold nanoparticle (GNP)-based colorimetric biosensors have enormous potential in clinical diagnostic applications due to their simplicity, versatility, and unique optical properties. In this study, we have developed an alendronate functionalized gold nanoparticle (GNP-ALD) system for the measurement of Ca2+ ion concentration in biological samples...
May 22, 2018: Nanoscale
https://www.readbyqxmd.com/read/29785630/calcium-channels-rho-kinase-protein-kinase-c-and-phospholipase-c-pathways-mediate-mercury-chloride-induced-myometrial-contractions-in-rats
#2
Swati Koli, Atul Prakash, Soumen Choudhury, Rajesh Mandil, Satish K Garg
Adverse effects of mercury on female reproduction are reported; however, its effect on myogenic activity of uterus and mechanism thereof is obscure. Present study was undertaken to unravel the mechanistic pathways of mercuric chloride (HgCl2 )-induced myometrial contraction in rats. Isometric tension in myometrial strips of rats following in vitro exposure to HgCl2 was recorded using data acquisition system-based physiograph. HgCl2 produced concentration-dependent (10 nM-100 μM) uterotonic effect which was significantly (p < 0...
May 21, 2018: Biological Trace Element Research
https://www.readbyqxmd.com/read/29785139/blockage-of-store-operated-ca-2-entry-antagonizes-epstein-barr-virus-promoted-angiogenesis-by-inhibiting-ca-2-signaling-regulated-vegf-production-in-nasopharyngeal-carcinoma
#3
Jiaxiang Ye, Junqi Huang, Qian He, Weilin Zhao, Xiaoying Zhou, Zhe Zhang, Yongqiang Li, Jiazhang Wei, Jinyan Zhang
Background: Epstein-Barr virus (EBV) actively contributes to the pathological process of nasopharyngeal carcinoma (NPC) by enabling NPC cells to acquire various capacities required for their malignant biological actions. Our earlier works demonstrated that EBV-encoded latent membrane protein 1 (LMP1) enhanced vascular endothelial growth factor (VEGF)-mediated angiogenesis by boosting store-operated Ca2+ entry (SOCE) upon extracellular epidermal growth factor (EGF) stimulation. However, the antagonistic effects of SOCE blockage on EBV-promoted angiogenesis must be appropriately evaluated in vivo, and the global effect of EBV infection on the EGF-elicited cytosolic Ca2+ signaling, which regulates VEGF-mediated angiogenesis remains to be further clarified...
2018: Cancer Management and Research
https://www.readbyqxmd.com/read/29785049/involvement-of-inhibitor-kappa-b-kinase-2-ikk2-in-the-regulation-of-vascular-tone
#4
Youngin Kwon, Soo-Kyoung Choi, Seonhee Byeon, Young-Ho Lee
Inhibitor kappa B kinase 2 (IKK2) plays an essential role in the activation of nuclear factor kappa B (NF-kB). Recently, it has been suggested that IKK2 acts as a myosin light chain kinase (MLCK) and contributes to vasoconstriction in mouse aorta. However, the underlying mechanisms are still unknown. Therefore, we investigated whether IKK2 acts as a MLCK or regulates the activity of myosin light chain phosphatase (MLCP). Pressure myograph was used to measure vascular tone in rat mesenteric arteries. Immunofluorescence staining was performed to identify phosphorylation levels of MLC (ser19), MYPT1 (thr853 and thr696) and CPI-17 (thr38)...
May 21, 2018: Laboratory Investigation; a Journal of Technical Methods and Pathology
https://www.readbyqxmd.com/read/29784361/calcium-selective-electrodes-based-on-photo-cured-polyurethane-acrylate-membranes-covalently-attached-to-methacrylate-functionalized-poly-3-4-ethylenedioxythiophene-as-solid-contact
#5
Cristina Ocaña, Natalia Abramova, Andrey Bratov, Tom Lindfors, Johan Bobacka
We report here the fabrication of solid-contact calcium-selective electrodes (Ca2+ -SCISEs) made of a polyurethane acrylate ion-selective membrane (ISM) that was covalently attached to the underlying ion-to-electron transducer (solid-contact). Methacrylate-functionalized poly(3,4-ethylenedioxythiophene) (Meth-PEDOT) and Meth-PEDOT films containing either multiwalled carbon nanotubes (MWCNT) or carboxylated MWCNT (cMWCNT) were used as solid contacts. The solid contacts were deposited by drop-casting on screen-printed electrodes and characterized by cyclic voltammetry (CV), electrochemical impedance spectroscopy (EIS) and potentiometry...
August 15, 2018: Talanta
https://www.readbyqxmd.com/read/29784347/simultaneous-sampling-of-dissolved-orthophosphate-and-ammonium-in-freshwaters-using-diffusive-gradients-in-thin-films-with-a-mixed-binding-phase
#6
Zhongmin Feng, Ni Wang, Manli He, Lan Yang, Yun Wang, Ting Sun
A new diffusive gradients in thin films (DGT) technique with a micro-sized biochar-zeolite-zirconium oxide mixed binding phase is described for simultaneous sampling of dissolved orthophosphate and ammonium in freshwaters. The performance of mixed binding layer (MBL) DGT technique was characterized by evaluation of the following parameters: (1) the adsorption efficiency of phosphate and ammonium by the mixed binding phase, (2) the elution efficiency, (3) the time-series accumulation, and (4) the influences of pH, coexisting cations and DOC on DGT uptake...
August 15, 2018: Talanta
https://www.readbyqxmd.com/read/29784322/peanut-flour-aggregation-with-polyphenolic-extracts-derived-from-peanut-skin-inhibits-ige-binding-capacity-and-attenuates-rbl-2h3-cells-degranulation-via-mapk-signaling-pathway
#7
Rishipal R Bansode, Nathalie J Plundrich, Priscilla D Randolph, Mary Ann Lila, Leonard L Williams
This study investigates the anti-allergic properties of peanut skin polyphenols (PSP)-enriched peanut (PN) protein aggregates. PSP was blended with PN flour at concentrations of 5, 10, 15, 30, and 40% (w/w). Rat basophil leukemia cells (RBL-2H3) were sensitized with either anti-DNP-IgE or PN-allergic plasma followed by co-exposure to unmodified PN flour (control) or PSP-PN protein aggregates and Ca2+ ionophore, ionomycin. Immunoblotting and staining were performed to measure the IgE binding capacity of PSP-PN aggregates...
October 15, 2018: Food Chemistry
https://www.readbyqxmd.com/read/29783772/acid-sphingomyelinase-promotes-cellular-internalization-of-clostridium-perfringens-iota-toxin
#8
Masahiro Nagahama, Masaya Takehara, Kazuaki Miyamoto, Kazumi Ishidoh, Keiko Kobayashi
Clostridium perfringens iota-toxin is a binary actin-ADP-ribosylating toxin composed of the enzymatic component Ia and receptor binding component Ib. Ib binds to a cell surface receptor, forms Ib oligomer in lipid rafts, and associates with Ia. The Ia-Ib complex then internalizes by endocytosis. Here, we showed that acid sphingomyelinase (ASMase) facilitates the cellular uptake of iota-toxin. Inhibitions of ASMase and lysosomal exocytosis by respective blockers depressed cell rounding induced by iota-toxin...
May 20, 2018: Toxins
https://www.readbyqxmd.com/read/29783744/interplay-between-er-ca-2-binding-proteins-stim1-and-stim2-is-required-for-store-operated-ca-2-entry
#9
Heather A Nelson, Colin A Leech, Richard F Kopp, Michael W Roe
Store-operated calcium entry (SOCE), a fundamentally important homeostatic and Ca2+ signaling pathway in many types of cells, is activated by the direct interaction of stromal interaction molecule 1 (STIM1), an endoplasmic reticulum (ER) Ca2+ -binding protein, with Ca2+ -selective Orai1 channels localized in the plasma membrane. While much is known about the regulation of SOCE by STIM1, the role of stromal interaction molecule 2 (STIM2) in SOCE remains incompletely understood. Here, using clustered regularly interspaced short palindromic repeats -CRISPR associated protein 9 (CRISPR-Cas9) genomic editing and molecular imaging, we investigated the function of STIM2 in NIH 3T3 fibroblast and αT3 cell SOCE...
May 19, 2018: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/29783655/gdf11-modulates-ca-2-dependent-smad2-3-signaling-to-prevent-cardiomyocyte-hypertrophy
#10
Javier Duran, Mayarling Francisca Troncoso, Daniel Lagos, Sebastian Ramos, Gabriel Marin, Manuel Estrada
Growth differentiation factor 11 (GDF11), a member of the transforming growth factor-β family, has been shown to act as a negative regulator in cardiac hypertrophy. Ca2+ signaling modulates cardiomyocyte growth; however, the role of Ca2+ -dependent mechanisms in mediating the effects of GDF11 remains elusive. Here, we found that GDF11 induced intracellular Ca2+ increases in neonatal rat cardiomyocytes and that this response was blocked by chelating the intracellular Ca2+ with BAPTA-AM or by pretreatment with inhibitors of the inositol 1,4,5-trisphosphate (IP₃) pathway...
May 18, 2018: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/29783646/pharmacological-strategies-for-manipulating-plant-ca-2-signalling
#11
REVIEW
Kjell De Vriese, Alex Costa, Tom Beeckman, Steffen Vanneste
Calcium is one of the most pleiotropic second messengers in all living organisms. However, signalling specificity is encoded via spatio-temporally regulated signatures that act with surgical precision to elicit highly specific cellular responses. How this is brought about remains a big challenge in the plant field, in part due to a lack of specific tools to manipulate/interrogate the plant Ca2+ toolkit. In many cases, researchers resort to tools that were optimized in animal cells. However, the obviously large evolutionary distance between plants and animals implies that there is a good chance observed effects may not be specific to the intended plant target...
May 18, 2018: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/29783109/anti-inflammatory-potential-of-alginic-acid-from-sargassum-horneri-against-urban-aerosol-induced-inflammatory-responses-in-keratinocytes-and-macrophages
#12
I P Shanura Fernando, Thilina U Jayawardena, K K Asanka Sanjeewa, Lei Wang, You-Jin Jeon, Won Woo Lee
The airborne particulate pollutants originating in the deserts of Mongolia and China which becomes contaminated with industrial effluents and traffic emissions while moving with the wind currents towards East Asia has recently become a serious environmental and health issue in the region. They cause asthma, collateral lung tissue damage, oxidative stress, allergic reactions, and inflammation. The current study was undertaken to evaluate the protective effects of alginate extracted from the invasive alga Sargassum horneri (SHA) against fine dust collected from Beijing, China (Chinese fine dust; CFD)...
May 18, 2018: Ecotoxicology and Environmental Safety
https://www.readbyqxmd.com/read/29783071/mef2a-regulates-calpain-3-expression-in-l6-myoblasts
#13
Ronghua Wu, Jun Wang, Jian Yao, Zhangji Dong, Yan Liu, Mei Liu
Calpain 3 (Capn3), a skeletal muscle-specific member of the calpain family, executes some non-proteolytic functions besides its role as a Ca2+ -regulated proteolytic enzyme. Previously, we found that changes in Capn3 expression were linearly correlated with the degree of muscular atrophy following reversible sciatic nerve injury and that knockdown of Capn3 gene expression promoted myoblast differentiation. While the regulation of capn3 gene expression is interesting, transcriptional regulation of Capn3 is still unclear...
May 18, 2018: Gene
https://www.readbyqxmd.com/read/29782955/loss-of-calretinin-and-parvalbumin-positive-interneurones-in-the-hippocampal-ca1-of-aged-alzheimer-s-disease-mice
#14
Fatima Zallo, Emanuela Gardenal, Alexei Verkhratsky, José Julio Rodríguez
Neuronal degeneration associated with Alzheimer's disease (AD), is linked to impaired calcium homeostasis and to changes in calcium-binding proteins (CBPs). The AD-related modification of neuronal CBPs remains controversial. Here we analyzed the presence and expression of calretinin (CR) and parvalbumin (PV) in the hippocampal CA1 neurones of 18 months old 3xTg-AD mice compared to non-Tg animals. We found a layer specific decrease in number of interneurones expressing CR and PV (by 33.7% and 52%, respectively)...
May 18, 2018: Neuroscience Letters
https://www.readbyqxmd.com/read/29782876/chloride-cotransporter-kcc2-is-essential-for-gabaergic-inhibition-in-the-scn
#15
A H O Olde Engberink, J H Meijer, S Michel
One of the principal neurotransmitters of the central nervous system is GABA. In the adult brain, GABA is predominantly inhibitory, but there is growing evidence indicating that GABA can shift to excitatory action depending on environmental conditions. In the mammalian central circadian clock of the suprachiasmatic nucleus (SCN) GABAergic activity shifts from inhibition to excitation when animals are exposed to long day photoperiod. The polarity of the GABAergic response (inhibitory versus excitatory) depends on the GABA equilibrium potential determined by the intracellular Cl- concentration ([Cl- ]i )...
May 18, 2018: Neuropharmacology
https://www.readbyqxmd.com/read/29782837/phenylalanine-and-tryptophan-stimulate-gastrin-and-somatostatin-secretion-and-h-k-atpase-activity-in-pigs-through-calcium-sensing-receptor
#16
Yihan Xian, Xiuying Zhao, Chao Wang, Cuicui Kang, Liren Ding, Weiyun Zhu, Suqin Hang
In rodents and humans, aromatic amino acids increase gut hormone secretion and H+ -K+ -ATPase activity by modulating calcium-sensing receptor (CaSR). However, the role of CaSR and its related signaling molecules in amino acid-induced gut hormone secretion in swine has not been investigated. Here, we examined whether a CaSR-dependent pathway modulated gastrin and somatostatin (SS) secretion and H+ -K+ -ATPase activity in pigs. Perfusion of pig stomach tissues in the presence of extracellular 80 mM l-phenylalanine (Phe) or 20 mM l-tryptophan (Trp) and a CaSR agonist cinacalcet triggered gastrin and SS secretion and H+ -K+ -ATPase activity (P < 0...
May 18, 2018: General and Comparative Endocrinology
https://www.readbyqxmd.com/read/29782207/the-benzodiazepine-anesthetic-midazolam-prevents-hyperglycemia-induced-microvascular-leakage-in-the-retinas-of-diabetic-mice
#17
Yeon-Ju Lee, Minsoo Kim, Jee-Yeon Lee, Se-Hui Jung, Hye-Yoon Jeon, Seung Ah Lee, Seongsik Kang, Eun-Taek Han, Won Sun Park, Seok-Ho Hong, Young-Myeong Kim, Kwon-Soo Ha
We investigated the beneficial effects of midazolam against vascular endothelial growth factor (VEGF)-induced vascular leakage and its molecular mechanism of action in human retinal endothelial cells (HRECs) and the retinas of diabetic mice. Midazolam inhibited VEGF-induced elevation of intracellular Ca2+ , generation of reactive oxygen species (ROS), and transglutaminase activation in HRECs; these effects were reversed by the GABA, type A (GABAA ) receptor antagonist flumazenil but not by the translocator protein antagonist PK11195...
May 21, 2018: FASEB Journal: Official Publication of the Federation of American Societies for Experimental Biology
https://www.readbyqxmd.com/read/29781984/mitochondrial-ca2-retention-capacity-assay-and-ca2-triggered-mitochondrial-swelling-assay
#18
Wei Li, Chen Zhang, Xiulian Sun
The production of ATP by oxidative phosphorylation is the primary function of mitochondria. Mitochondria in higher eukaryotes also participate in cytosolic Ca2+ buffering, and the ATP production in mitochondrial can be mediated by intramitochondrial free Ca2+ concentration. Ca2+ retention capacity can be regarded as the capability of mitochondria to retain calcium in the mitochondrial matrix. Accumulated intracellular Ca2+ leads to the permeability of the inner mitochondrial membrane, termed the opening of mitochondrial permeability transition pore (mPTP), which leads to the leakage of molecules with a molecular weight less than 1...
May 1, 2018: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/29781506/articular-cartilage-protection-in-ctsk-mice-is-associated-with-cellular-and-molecular-changes-in-subchondral-bone-and-cartilage-matrix
#19
Fabiana N Soki, Ryu Yoshida, David N Paglia, Le T Duong, Marc F Hansen, Hicham Drissi
Osteoarthritis (OA) is a degenerative disease and a major cause of chronic disability in aging individuals. Cathepsin K (CatK), encoded by the Ctsk gene, has been implicated in the pathogenesis of pycnodysostosis and osteoporosis. The use of a selective inhibitor of CatK was recently shown to delay OA progression in rabbits. However, the cellular mechanisms underlying these protective effects remain unexplored. We examined articular cartilage maintenance and joint bone remodeling using Ctsk null mice (Ctsk-/- ) which underwent destabilization of the medial meniscus (DMM)...
May 21, 2018: Journal of Cellular Physiology
https://www.readbyqxmd.com/read/29781206/electromembrane-extraction-and-capillary-electrophoresis-with-capacitively-coupled-contactless-conductivity-detection-multi-extraction-capabilities-to-analyse-trace-metals-from-saline-samples
#20
Macarena Silva, Carolina Mendiguchía, Carlos Moreno, Pavel Kubáň
Simultaneous electromembrane extraction (EME) of six trace metal cations (Cu2+ , Zn2+ , Co2+ , Ni2+ , Pb2+ , Cd2+ ) from saline samples was investigated. Capillary electrophoresis (CE) with capacitively coupled contactless conductivity detection (C4 D) was used to determine the metals in acceptor solutions due to its excellent compatibility with the minute volumes of acceptor solutions. Bis(2-ethylhexyl)phosphate (DEHPA) was selected as a suitable non-selective modifier for EME transport of target metal cations...
May 21, 2018: Electrophoresis
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