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https://www.readbyqxmd.com/read/28934222/endogenous-rgs14-is-a-cytoplasmic-nuclear-shuttling-protein-that-localizes-to-juxtanuclear-membranes-and-chromatin-rich-regions-of-the-nucleus
#1
Mary Rose Branch, John R Hepler
Regulator of G protein signaling 14 (RGS14) is a multifunctional scaffolding protein that integrates G protein and H-Ras/MAPkinase signaling pathways to regulate synaptic plasticity important for hippocampal learning and memory. However, to date, little is known about the subcellular distribution and roles of endogenous RGS14 in a neuronal cell line. Most of what is known about RGS14 cellular behavior is based on studies of tagged, recombinant RGS14 ectopically overexpressed in unnatural host cells. Here, we report for the first time a comprehensive assessment of the subcellular distribution and dynamic localization of endogenous RGS14 in rat B35 neuroblastoma cells...
2017: PloS One
https://www.readbyqxmd.com/read/28934042/taking-a-stand-living-the-art-of-humanbecoming
#2
Karen Carroll
Living the art is a disciplinary grounded knowledge contained in the humanbecoming paradigm honoring persons with true presence. The evidence and healthcare milieu may change; however, true presence brings new insights with illuminating meaning, shifting rhythms, and inspiring transcendence as nurses bear witness to the changing beliefs or patterns of persons, families, and communities. The author in this paper reviews a journey that focuses on living the art.
October 2017: Nursing Science Quarterly
https://www.readbyqxmd.com/read/28934034/transfiguring-light-and-symphonic-blooming-a-wabi-sabi-story-of-humanbecoming-and-human-caring
#3
Lois S Kelley
Transfiguring Light and Symphonic Blooming is word-art to describe the flowering of the humanbecoming and human caring schools of thought over time. A Wabi Sabi story journey was created to turn the tide in a world where the building of a unique body of knowledge for nursing has fallen into disfavor with nursing itself. Using nature-aligned paintings and a Japanese story-art aesthetic with poetry, the beauty of falling away to unitary essentials and holding the value of "both-and" in unitary consciousness was illuminated...
October 2017: Nursing Science Quarterly
https://www.readbyqxmd.com/read/28933928/nursing-and-the-public-health-legacies-of-the-russian-revolution
#4
Susan Grant
The centenary of the October Revolution in 1917 provides a timely opportunity to assess the legacies of that event. I examine the role of the revolution in public health with a focus on nursing, assessing the Imperial Russian health care system, the development of Soviet nursing, and current plans for nursing and public health care in Putin's Russia. Analyzing nursing shows that there was a great deal of continuity in terms of medical personnel and ideas on how public health care service in Russia should operate...
September 21, 2017: American Journal of Public Health
https://www.readbyqxmd.com/read/28933747/facilitators-of-physical-activity-voices-of-adolescents-in-a-disadvantaged-community
#5
Linus Jonsson, Christina Berg, Christel Larsson, Peter Korp, Eva-Carin Lindgren
Despite increasing socioeconomic inequalities in the health and well-being of adolescents, the voices of adolescents in disadvantaged communities regarding facilitators of physical activity (PA) have received relatively little attention. In response, the purpose of this study was to illuminate what adolescents in a multicultural community of low socioeconomic status (SES) in Sweden convey concerning facilitators of PA. Adolescents (n = 53, aged 12-13 years) were recruited from a school in a multicultural community of low SES in Sweden...
July 26, 2017: International Journal of Environmental Research and Public Health
https://www.readbyqxmd.com/read/28933727/venomics-of-remipede-crustaceans-reveals-novel-peptide-diversity-and-illuminates-the-venom-s-biological-role
#6
Björn M von Reumont, Eivind A B Undheim, Robin-Tobias Jauss, Ronald A Jenner
We report the first integrated proteomic and transcriptomic investigation of a crustacean venom. Remipede crustaceans are the venomous sister group of hexapods, and the venom glands of the remipede Xibalbanus tulumensis express a considerably more complex cocktail of proteins and peptides than previously thought. We identified 32 venom protein families, including 13 novel peptide families that we name xibalbins, four of which lack similarities to any known structural class. Our proteomic data confirm the presence in the venom of 19 of the 32 families...
July 26, 2017: Toxins
https://www.readbyqxmd.com/read/28933316/photo-activatable-akt-probe-a-new-tool-to-study-the-akt-dependent-physiopathology-of-cancer-cells
#7
Sanae Haga, Takeaki Ozawa, Naoki Morita, Mami Asano, Shigeki Jin, Michitaka Ozakia
Akt is commonly overexpressed and activated in cancer cells, and plays a pivotal role in cell survival, protection andchemo-resistance. Therefore, Akt is one of the target molecules in understanding characters of cancer cells and developing anti-cancer drugs. Here, we examined whether a newly developed photo-activatable Akt (PA-Akt) probe, based on a light-inducible protein interaction module of plant cryptochrome2 (CRY2) and cryptochrome-interacting basic-helix-loop-helix (CIB1), can regulate Akt-associated cell functions...
August 30, 2017: Oncology Research
https://www.readbyqxmd.com/read/28933078/-advance-in-biosynthesis-of-terpenoid-indole-alkaloids-and-its-regulation-in-catharanthus-roseus
#8
Xue-Jun Kuang, Cai-Xia Wang, Li-Qiu Zou, Xiao-Xuan Zhu, Chao Sun
Catharanthus roseus can produce a variety of terpenoid indole alkaloids (TIA), most of which exhibit strong pharmacological activities. Hence, biosynthesis and regulation of TIA have received recent attention. 3α (S)-strictosidine is an important node in TIA biosynthesis, which is a condensation product of secologanin and tryptamine. The former is produced in iridoid pathway, and the latter is produced in indole pathway. Vindoline and catharanthine, which are produced respectively by 3α (S)-strictosidine via multi-step enzymatic reaction, can form α-3, 4-anhydrovinblastine by the condensation reaction...
November 2016: Zhongguo Zhong Yao za Zhi, Zhongguo Zhongyao Zazhi, China Journal of Chinese Materia Medica
https://www.readbyqxmd.com/read/28933033/sleep-disturbance-after-tbi
#9
REVIEW
Surendra Barshikar, Kathleen R Bell
PURPOSE OF REVIEW: Present relevant literature to update knowledge on sleep science, identify common sleep disturbances seen in TBI, discuss evidence for available treatment options, and illuminate future areas for research. RECENT FINDINGS: Sleep disturbances, including insomnia, circadian rhythm disturbances, and sleep apnea, are prevalent for all severities of traumatic brain injury (TBI), can be chronic, and affect both rehabilitation and recovery from the TBI...
September 20, 2017: Current Neurology and Neuroscience Reports
https://www.readbyqxmd.com/read/28932848/exploration-of-significant-influences-of-the-operating-conditions-on-the-local-o2-transport-in-proton-exchange-membrane-fuel-cells-pemfcs
#10
Shuiyun Shen, Xiaojing Cheng, Chao Wang, Xiaohui Yan, Changchun Ke, Jiewei Yin, Junliang Zhang
A drastic reduction of the Pt loading in the cathode catalyst layers (CCLs) of proton exchange membrane fuel cells (PEMFCs) is much desired. However, a decrease in Pt loading inevitably leads to an unexpected increase of local O2 transport resistance (rLocal) and severely weakens the fuel cell performance, particularly at high current densities. Thus, it is both urgent and meaningful to explore the impacts of the operating conditions on rLocal in CCLs and therefore to clarify the intrinsic mechanism. Herein, we systematically explore the influences of the operating conditions, in terms of the dry O2 mole fraction, the relative humidity, the operating pressure and the temperature on rLocal using limiting current measurements combined with mathematical calculations...
September 21, 2017: Physical Chemistry Chemical Physics: PCCP
https://www.readbyqxmd.com/read/28932569/endobronchial-ultrasound-guidance-for-interstitial-photodynamic-therapy-of-locally-advanced-lung-cancer-a-new-interventional-concept
#11
Kassem Harris, Emily Oakley, David Bellnier, Gal Shafirstein
Recent advances in interventional pulmonology led to a significant expansion of the diagnostic and therapeutic role of endobronchial ultrasound. In this paper, we describe a new concept for using endobronchial ultrasound to guide interstitial photodynamic therapy (PDT). For this purpose, we conducted in vitro and in vivo experiments using a phantom and animal models, respectively. A new 0.5 mm optical fiber, with cylindrical diffuser end, was used to deliver the therapeutic light through the 21-gauge endobronchial ultrasound needle...
August 2017: Journal of Thoracic Disease
https://www.readbyqxmd.com/read/28931889/fast-fourier-single-pixel-imaging-via-binary-illumination
#12
Zibang Zhang, Xueying Wang, Guoan Zheng, Jingang Zhong
Fourier single-pixel imaging (FSI) employs Fourier basis patterns for encoding spatial information and is capable of reconstructing high-quality two-dimensional and three-dimensional images. Fourier-domain sparsity in natural scenes allows FSI to recover sharp images from undersampled data. The original FSI demonstration, however, requires grayscale Fourier basis patterns for illumination. This requirement imposes a limitation on the imaging speed as digital micro-mirror devices (DMDs) generate grayscale patterns at a low refreshing rate...
September 20, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28931853/flexible-polarimetric-probe-for-3%C3%A2-%C3%A3-%C3%A2-3-mueller-matrix-measurements-of-biological-tissue
#13
Sarah Forward, Adam Gribble, Sanaz Alali, Andras A Lindenmaier, I Alex Vitkin
Polarimetry is a noninvasive method that uses polarised light to assess biophysical characteristics of tissues. A series of incident polarisation states illuminates a biological sample, and analysis of sample-altered polarisation states enables polarimetric tissue assessment. The resultant information can, for example, help quantitatively differentiate healthy from pathologic tissue. However, most bio-polarimetric assessments are performed using free-space optics with bulky optical components. Extension to flexible fibre-based systems is clinically desirable, but is challenging due to polarisation-altering properties of optical fibres...
September 20, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28931767/musical-instrument-associated-health-issues-and-their-management
#14
Kae Okoshi, Taro Minami, Masahiro Kikuchi, Yasuko Tomizawa
Playing musical instruments can bring joy to people, but can also cause a wide variety of health issues that range from mild disorders to potentially fatal conditions. Although sports medicine is an established medical subspecialty, relatively few studies have investigated the health issues associated with musical instruments. Here we present an overview of these health issues. These include infections due to microorganisms, allergic reactions, as well as mechanical injuries from sustained high pressures within the oral, mediastinal, thoracic, and abdominal cavities...
2017: Tohoku Journal of Experimental Medicine
https://www.readbyqxmd.com/read/28931638/complex-systems-biology
#15
REVIEW
Avi Ma'ayan
Complex systems theory is concerned with identifying and characterizing common design elements that are observed across diverse natural, technological and social complex systems. Systems biology, a more holistic approach to study molecules and cells in biology, has advanced rapidly in the past two decades. However, not much appreciation has been granted to the realization that the human cell is an exemplary complex system. Here, I outline general design principles identified in many complex systems, and then describe the human cell as a prototypical complex system...
September 2017: Journal of the Royal Society, Interface
https://www.readbyqxmd.com/read/28931526/cellubrevin-vesicle-associated-membrane-protein-3-vamp-3-mediated-endocytosis-and-trafficking-regulate-platelet-functions
#16
Meenakshi Banerjee, Smita Joshi, Jinchao Zhang, Carole L Moncman, Shilpi Yadav, Beth A Bouchard, Brian Storrie, Sidney W Whiteheart
Endocytosis is key to fibrinogen (Fg) uptake, trafficking of integrins (αIIbβ3, αvβ3) and purinergic receptors (P2Y1, P2Y12), and thus for normal platelet function. However, the molecular machinery required and possible trafficking routes are still ill-defined. To further identify elements of the platelet endocytic machinery, we examined the role of a vesicle-residing, Soluble N-ethylmaleimide Factor Attachment Protein Receptor (v-SNARE) called Cellubrevin/Vesicle-Associated Membrane Protein-3 (VAMP-3) in platelet function...
September 20, 2017: Blood
https://www.readbyqxmd.com/read/28931284/one-step-synthesis-of-fluorescent-boron-nitride-quantum-dots-via-a-hydrothermal-strategy-using-melamine-as-nitrogen-source-for-the-detection-of-ferric-ions
#17
Bingbing Huo, Bingping Liu, Tao Chen, Liang Cui, Gengfang Xu, Mengli Liu, Jingquan Liu
A facile and effective approach for the preparation of functionalized born nitride quantum dots (BNQDs) with blue fluorescence was explored by the hydrothermal treatment of the mixture of boric acid and melamine at 200 oC for 15 h. The as-prepared BNQDs were characterized by transmission electron microscopy (TEM), high-resolution TEM (HRTEM), atomic force microscopy (AFM), X-ray photoelectron spectroscopy (XPS), UV-vis spectroscopy and fluorescence spectroscopy. The single layered BNQDs with the average size of 3 nm showed a blue light emission under the illumination of the UV light...
September 20, 2017: Langmuir: the ACS Journal of Surfaces and Colloids
https://www.readbyqxmd.com/read/28931000/trapping-of-micro-particles-in-nanoplasmonic-optical-lattice
#18
Dinesh Bhalothia, Ya-Tang Yang
The plasmonic optical tweezer has been developed to overcome the diffraction limits of the conventional far field optical tweezer. Plasmonic optical lattice consists of an array of nanostructures, which exhibit a variety of trapping and transport behaviors. We report the experimental procedures to trap micro-particles in a simple square nanoplasmonic optical lattice. We also describe the optical setup and the nanofabrication of a nanoplasmonic array. The optical potential is created by illuminating an array of gold nanodiscs with a Gaussian beam of 980 nm wavelength, and exciting plasmon resonance...
September 5, 2017: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/28930487/emerging-roles-of-inflammasomes-in-acute-pneumonia
#19
Sangeetha Ravi Kumar, Sagar Paudel, Laxman Ghimire, Scott Bergeron, Shanshan Cai, Rachel L Zemans, Gregory P Downey, Samithamby Jeyaseelan
Pneumonia is responsible for a substantial burden on healthcare worldwide and the single largest cause for death of infants globally. Pneumonia is caused by a variety of pathogenic microorganisms including bacteria, viruses and fungi. The lung has developed complex innate immune mechanisms to combat these infections while minimizing tissue damage. One essential mechanism that drives the initial innate immune response is sensing a broad spectrum of stimuli in the cytosol by inflammasomes. Inflammasomes are composed of NLR and non-NLR proteins, and activate caspase-1 that results in proinflammatory cytokine activation...
September 20, 2017: American Journal of Respiratory and Critical Care Medicine
https://www.readbyqxmd.com/read/28930347/a-paper-triboelectric-nanogenerator-for-self-powered-electronic-systems
#20
Yanchao Mao, Nan Zhang, Yingjie Tang, Meng Wang, Mingju Chao, Erjun Liang
Paper, as one of the most important inventions of mankind, is still widely used in our daily life. In this study, the paper is explored as a platform for power sources based on the universally known triboelectric friction. A novel paper triboelectric nanogenerator (P-TENG) was successfully developed, which could harvest mechanical energy from ambient sources and generate considerable electrical energy. The maximum power density of the P-TENG reached 53 W m(-2). In addition, the P-TENG possesses a natural advantage of being able to be integrated within a book, and thus can effectively convert mechanical energy from the action of turning book pages into electricity...
September 20, 2017: Nanoscale
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