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https://www.readbyqxmd.com/read/27896424/dynamic-expression-analysis-of-early-response-genes-induced-by-potato-virus-y-in-pvy-resistant-nicotiana-tabacum
#1
Shuai Chen, Fengxia Li, Dan Liu, Caihong Jiang, Lijie Cui, Lili Shen, Guanshan Liu, Aiguo Yang
Dynamic transcriptional changes of the host early responses genes were detected in PVY-resistant tobacco varieties infected with Potato virus Y; PVY resistance is a complex process that needs series of stress responses. Potato virus Y (PVY) causes a severe viral disease in cultivated crops, especially in Solanum plants. To understand the molecular basis of plant responses to the PVY stress, suppression subtractive hybridization (SSH) and microarray approaches were combined to identify the potentially important or novel genes that were involved in early stages (12 h, 1, 2, 3, 5, 8 days) of tobacco response to PVY infection...
November 28, 2016: Plant Cell Reports
https://www.readbyqxmd.com/read/27886491/interplay-of-site-and-bond-electron-phonon-coupling-in-one-dimension
#2
Martin Hohenadler
The interplay of bond and charge correlations is studied in a one-dimensional model with both Holstein and Su-Schrieffer-Heeger (SSH) couplings to quantum phonons. The problem is solved exactly by quantum Monte Carlo simulations. If one of the couplings dominates, the ground state is a Peierls insulator with long-range bond or charge order. At weak coupling, the results suggest a spin-gapped and repulsive metallic phase arising from the competing order parameters and lattice fluctuations. Such a phase is absent from the pure SSH model even for quantum phonons...
November 11, 2016: Physical Review Letters
https://www.readbyqxmd.com/read/27872628/proteomics-and-ssh-analyses-of-ala-promoted-fruit-coloration-and-evidence-for-the-involvement-of-a-mads-box-gene-mdmads1
#3
Xinxin Feng, Yuyan An, Jie Zheng, Miao Sun, Liangju Wang
Skin color is a key quality attribute of fruits and how to improve fruit coloration has long been a major concern. 5-Aminolevulinic acid (ALA), a natural plant growth regulator, can significantly increase anthocyanin accumulation in fruit skin and therefore effectively improve coloration of many fruits, including apple. However, the molecular mechanism how ALA stimulates anthocyanin accumulation in fruit skin remains unknown. Here, we investigated the impact of ALA on apple skin at the protein and mRNA levels...
2016: Frontiers in Plant Science
https://www.readbyqxmd.com/read/27862175/epistemology-culture-justice-and-power-non-bioscientific-knowledge-for-medical-training
#4
Ayelet Kuper, Paula Veinot, Jennifer Leavitt, Sarah Levitt, Amanda Li, Jeannette Goguen, Martin Schreiber, Lisa Richardson, Cynthia R Whitehead
CONTEXT: While medical curricula were traditionally almost entirely comprised of bioscientific knowledge, widely accepted competency frameworks now make clear that physicians must be competent in far more than biomedical knowledge and technical skills. For example, of the influential CanMEDS roles, six are conceptually based in the social sciences and humanities (SSH). Educators frequently express uncertainty about what to teach in this area. This study concretely identifies the knowledge beyond bioscience needed to support the training of physicians competent in the six non-Medical Expert CanMEDS roles...
November 15, 2016: Medical Education
https://www.readbyqxmd.com/read/27849566/effects-of-sacubitril-valsartan-lcz696-on-natriuresis-diuresis-blood-pressures-and-nt-probnp-in-salt-sensitive-hypertension
#5
Tzung-Dau Wang, Ru-San Tan, Hae-Young Lee, Sang-Hyun Ihm, Moo-Yong Rhee, Brian Tomlinson, Parasar Pal, Fan Yang, Elizabeth Hirschhorn, Margaret F Prescott, Markus Hinder, Thomas H Langenickel
: Salt-sensitive hypertension (SSH) is characterized by impaired sodium excretion and subnormal vasodilatory response to salt loading. Sacubitril/valsartan (LCZ696) was hypothesized to increase natriuresis and diuresis and result in superior blood pressure control compared with valsartan in Asian patients with SSH. In this randomized, double-blind, crossover study, 72 patients with SSH received sacubitril/valsartan 400 mg and valsartan 320 mg once daily for 4 weeks each. SSH was diagnosed if the mean arterial pressure increased by ≥10% when patients switched from low (50 mmol/d) to high (320 mmol/d) sodium diet...
November 14, 2016: Hypertension
https://www.readbyqxmd.com/read/27813556/overexpression-of-a-glycine-rich-protein-gene-in-lablab-purpureus-improves-abiotic-stress-tolerance
#6
L M Yao, Y N Jiang, X X Lu, B Wang, P Zhou, T L Wu
Glycine-rich protein (GRP) is involved in the response to abiotic and biotic stresses in plants. A novel GRP gene in Lablab purpureus has been identified. The cDNA of LpGRP was obtained from an SSH library constructed with root tissues of L. purpureus MEIDOU 2012 by waterholding for 10 days. The function of LpGRP was also evaluated in Arabidopsis. The cDNA of LpGRP has 555 bp and encodes a 184-amino acid protein. LpGRP was induced by drought and improved tolerance to abiotic stress. In LpGRP overexpressing Arabidopsis, the tolerance of transgenic seedlings to drought and salt was improved, and transgenic seeds showed insensitivity to both ABA and NaCl...
October 24, 2016: Genetics and Molecular Research: GMR
https://www.readbyqxmd.com/read/27786294/molecular-cloning-and-expression-of-an-encoding-galactinol-synthase-gene-angols1-in-seedling-of-ammopiptanthus-nanus
#7
YuDong Liu, Li Zhang, LiJing Chen, Hui Ma, YanYe Ruan, Tao Xu, ChuanQiang Xu, Yi He, MingFang Qi
Based on the galactinol synthase (AnGolS1) fragment sequence from a cold-induced Suppression Subtractive Hybridization (SSH) library derived from Ammopiptanthus nanus (A. nanus) seedlings, AnGolS1 mRNA (including the 5' UTR and 3' UTR) (GenBank accession number: GU942748) was isolated and characterized by rapid amplification of cDNA ends polymerase chain reaction (RACE-PCR). A substrate reaction test revealed that AnGolS1 possessed galactinol synthase activity in vitro and could potentially be an early-responsive gene...
October 27, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27751917/in%C3%A2-vivo-activity-and-the-transcriptional-regulatory-mechanism-of-the-antimicrobial-peptide-sphyastatin-in-scylla-paramamosain
#8
Zhong-Guo Shan, Ke-Xin Zhu, Fang-Yi Chen, Jie Liu, Bei Chen, Kun Qiao, Hui Peng, Ke-Jian Wang
A new gene homologous to the reported antimicrobial peptide (AMP) hyastatin from Hyas araneus was screened in the SSH library constructed from the hemocytes of Scylla paramamosain, and named SpHyastatin. In vivo study showed that SpHyastatin was predominantly expressed in hemocytes of S. paramamosain. With the challenge of either Vibrio parahaemolyticus or lipopolysaccharide (LPS), SpHyastatin showed a positive response, meaning that it was probably involved in the immune reaction against bacterial infection in vivo...
December 2016: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/27744104/suppressive-subtractive-hybridization-reveals-different-gene-expression-between-high-and-low-virulence-strains-of-cladosporium-cladosporioides
#9
Yu Gu, Yanfang Liu, Sanjie Cao, Xiaobo Huang, Zhicai Zuo, Shumin Yu, Junliang Deng, Chunbang Ding, Ming Yuan, Liuhong Shen, Rui Wu, Yiping Wen, Zhihua Ren, Qin Zhao, Guangneng Peng, Zhijun Zhong, Chengdong Wang, Xiaoping Ma
Cladosporium cladosporioides is a ubiquitous fungus, causing infections in plants, humans, and animals. Suppression subtractive hybridization (SSH) and quantitative real-time PCR (qRT-PCR) were used in this study to identify differences in gene expression between two C. cladosporioides strains, the highly virulent Z20 strain and the lowly virulent Zt strain. A total of 61 unigenes from the forward library and 42 from the reverse library were identified. Gene ontology (GO) analysis showed that these genes were involved in various biological processes, cellular components and molecular functions...
October 13, 2016: Microbial Pathogenesis
https://www.readbyqxmd.com/read/27743114/expression-profile-and-in-silico-characterization-of-novel-rtf2h-gene-under-oxidative-stress-in-indian-catfish-clarias-magur-hamilton-1822
#10
Prabhaker Yadav, Ratnesh K Tripathi, Rajeev K Singh, Vindhya Mohindra
During the investigation of genes involved in the hypoxia tolerance, novel transcript, Replication Termination Factor 2 homologue (RTF2h), was found to be differentially expressed in brain of Clarias magur (previous name, C. batrachus) whose function was still undefined. Thus, present study was aimed to examine the transcriptional response of novel RTF2h gene, for its possible involvement in hypoxia tolerance in C. magur. Novel transcripts expressed under hypoxic stress were identified from ESTs obtained from SSH libraries of C...
October 14, 2016: Molecular Biology Reports
https://www.readbyqxmd.com/read/27742587/hypoxic-stress-responsive-genes-in-air-breathing-catfish-clarias-magur-hamilton-1822-and-their-possible-physiological-adaptive-function
#11
Vindhya Mohindra, Ratnesh Kumar Tripathi, Akanksha Singh, Ruchi Patangia, Rajeev Kumar Singh, Kuldeep Kumar Lal, Joy Krushna Jena
The Indian catfish, Clarias magur (previous name C. batrachus) is an air breathing fish, inhabitant of aquatic bodies characterized by low dissolved oxygen levels. It is exposed to hypoxic conditions in its natural habitat. Thus, it can be useful model to study the mechanism of hypoxia stress tolerance. In C. magur, molecular processes facilitating its adaptation to hypoxia stress remain largely unexplored, in part due to unavailability of genomic resources. The suppression subtractive hybridization technique (SSH) was employed to compare the differential expression of transcripts under experimental hypoxic conditions, to that of normoxic conditions...
December 2016: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/27696738/transcriptome-analysis-of-sugar-beet-root-maggot-tetanops-myopaeformis-genes-modulated-by-the-beta-vulgaris-host
#12
Haiyan Li, Ann C Smigocki
Sugar beet root maggot (SBRM, Tetanops myopaeformis von Röder) is a major but poorly understood insect pest of sugar beet (Beta vulgaris L.). The molecular mechanisms underlying plant defense responses are well documented, however, little information is available about complementary mechanisms for insect adaptive responses to overcome host resistance. To date, no studies have been published on SBRM gene expression profiling. Suppressive subtractive hybridization (SSH) generated more than 300 SBRM ESTs differentially expressed in the interaction of the pest with a moderately resistant (F1016) and a susceptible (F1010) sugar beet line...
October 3, 2016: Insect Science
https://www.readbyqxmd.com/read/27586658/subtractive-transcriptome-analysis-of-leaf-and-rhizome-reveals-differentially-expressed-transcripts-in-panax-sokpayensis
#13
Bhusan Gurung, Pardeep K Bhardwaj, Narayan C Talukdar
In the present study, suppression subtractive hybridization (SSH) strategy was used to identify rare and differentially expressed transcripts in leaf and rhizome tissues of Panax sokpayensis. Out of 1102 randomly picked clones, 513 and 374 high quality expressed sequenced tags (ESTs) were generated from leaf and rhizome subtractive libraries, respectively. Out of them, 64.92 % ESTs from leaf and 69.26 % ESTs from rhizome SSH libraries were assembled into different functional categories, while others were of unknown function...
September 1, 2016: Functional & Integrative Genomics
https://www.readbyqxmd.com/read/27582756/ssh-analysis-of-endosperm-transcripts-and-characterization-of-heat-stress-regulated-expressed-sequence-tags-in-bread-wheat
#14
Suneha Goswami, Ranjeet R Kumar, Kavita Dubey, Jyoti P Singh, Sachidanand Tiwari, Ashok Kumar, Shuchi Smita, Dwijesh C Mishra, Sanjeev Kumar, Monendra Grover, Jasdeep C Padaria, Yugal K Kala, Gyanendra P Singh, Himanshu Pathak, Viswanathan Chinnusamy, Anil Rai, Shelly Praveen, Raj D Rai
Heat stress is one of the major problems in agriculturally important cereal crops, especially wheat. Here, we have constructed a subtracted cDNA library from the endosperm of HS-treated (42°C for 2 h) wheat cv. HD2985 by suppression subtractive hybridization (SSH). We identified ~550 recombinant clones ranging from 200 to 500 bp with an average size of 300 bp. Sanger's sequencing was performed with 205 positive clones to generate the differentially expressed sequence tags (ESTs). Most of the ESTs were observed to be localized on the long arm of chromosome 2A and associated with heat stress tolerance and metabolic pathways...
2016: Frontiers in Plant Science
https://www.readbyqxmd.com/read/27578263/external-drains-versus-no-drains-after-burr-hole-evacuation-for-the-treatment-of-chronic-subdural-haematoma-in-adults
#15
REVIEW
Deqing Peng, Yongjian Zhu
BACKGROUND: Chronic subdural haematoma (CSDH) is one of the most common types of intracranial haematoma, and often occurs in older people. Burr-hole craniostomy, which is an evacuation through one or two burr holes drilled over the site of the haematoma, has been widely accepted as the most effective way to manage CSDH. Recurrences are a major problem and need reoperation, sometimes repeatedly. OBJECTIVES: To assess the effects and safety of the use of external drains versus no drains after burr-hole evacuation for the treatment of CSDH in adults...
2016: Cochrane Database of Systematic Reviews
https://www.readbyqxmd.com/read/27565983/a-r2r3-myb-transcription-factor-from-lablab-purpureus-induced-by-drought-increases-tolerance-to-abiotic-stress-in-arabidopsis
#16
Luming Yao, Yina Jiang, Xinxin Lu, Biao Wang, Pei Zhou, Tianlong Wu
Few regulators for drought tolerance have been identified in Lablab purpureus which is a multipurpose leguminous crop. The transcription factor MYB is involved in regulatory networks in response to abiotic and biotic stresses in plants. A novel R2R3-MYB factor in L. purpureus has been identified. An suppression subtraction hybridization (SSH) library was constructed using root tissues of L. purpureus MEIDOU 2012 from well-watered and water-stress treatments that were subjected to drought stress for 10 days...
October 2016: Molecular Biology Reports
https://www.readbyqxmd.com/read/27544199/function-of-hevea-brasiliensis-nac1-in-dehydration-induced-laticifer-differentiation-and-latex-biosynthesis
#17
Yuxin Cao, Jinling Zhai, Qichao Wang, Hongmei Yuan, Xi Huang
HbNAC1 is a transcription factor in rubber plants whose expression is induced by dehydration, leading to latex biosynthesis. Laticifer is a special tissue in Hevea brasiliensis where natural rubber is biosynthesized and accumulated. In young stems of epicormic shoots, the differentiation of secondary laticifers can be induced by wounding, which can be prevented when the wounding site is wrapped. Using this system, differentially expressed genes were screened by suppression subtractive hybridization (SSH) and macroarray analyses...
August 20, 2016: Planta
https://www.readbyqxmd.com/read/27521482/molecular-changes-in-skin-pigmented-lesions-of-the-coral-trout-plectropomus-leopardus
#18
Adélaïde Lerebours, Emma C Chapman, Michael J Sweet, Michelle R Heupel, Jeanette M Rotchell
A high prevalence of skin pigmented lesions of 15% was recently reported in coral trout Plectropomus leopardus, a commercially important marine fish, inhabiting the Great Barrier Reef. Herein, fish were sampled at two offshore sites, characterised by high and low lesion prevalence. A transcriptomic approach using the suppressive subtractive hybridisation (SSH) method was used to analyse the differentially expressed genes between lesion and normal skin samples. Transcriptional changes of 14 genes were observed in lesion samples relative to normal skin samples...
September 2016: Marine Environmental Research
https://www.readbyqxmd.com/read/27508835/-op-7c-03-klotho-gene-polymorphism-interacts-with-adducin-endogenous-ouabain-na-k-atpase-system-in-salt-sensitive-hypertension
#19
S Tentori, C Lanzani, L Citterio, E Brioni, M Simonini, L Zagato, E Messaggio, P Manunta
OBJECTIVE: The genetic basis of salt sensitive (SSH) hypertension consists in a very complex network of dynamic interaction among genetic-environmental factors that may change with aging. The existence of a regulatory genetic network (RGN) as triggering mechanism of SSH has been already postulated for Adducin-Endogenous Ouabain-Na+-K+ ATPase (ADD-EO-NKA) system. The observation that Klotho (KL) is a newly discovered aging suppressor gene can directly affect the Na+, K+-ATPase activity opening new possible interaction with the RGN...
September 2016: Journal of Hypertension
https://www.readbyqxmd.com/read/27464848/gene-expression-profiling-in-fish-toxicology-a-review
#20
Girish Kumar, Nancy D Denslow
In this review, we present an overview of transcriptomic responses to chemical exposures in a variety of fish species. We have discussed the use of several molecular approaches such as northern blotting, differential display reverse transcription-polymerase chain reaction (DDRT-PCR), suppression subtractive hybridization (SSH), real time quantitative PCR (RT-qPCR), microarrays, and next-generation sequencing (NGS) for measuring gene expression. These techniques have been mainly used to measure the toxic effects of single compounds or simple mixtures in laboratory conditions...
July 28, 2016: Reviews of Environmental Contamination and Toxicology
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