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https://www.readbyqxmd.com/read/29017938/wssv-responsive-gene-expression-under-the-influence-of-pmvrp15-suppression
#1
Phawida Tummamunkong, Phattarunda Jaree, Anchalee Tassanakajon, Kunlaya Somboonwiwat
The viral responsive protein 15 from black tiger shrimp Penaeus monodon (PmVRP15), is highly up-regulated and produced in the hemocytes of shrimp with white spot syndrome virus (WSSV) infection. To investigate the differential expression of genes from P. monodon hemocytes that are involved in WSSV infection under the influence of PmVRP15 expression, suppression subtractive hybridization (SSH) of PmVRP15-silenced shrimp infected with WSSV was performed. The 189 cDNAs clones of the forward library were generated by subtracting the cDNAs from WSSV-infected and PmVRP15 knockdown shrimp with cDNAs from WSSV-infected and GFP knockdown shrimp...
October 7, 2017: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/28971245/sacrospinous-ligament-fixation-for-hysteropexy-does-concomitant-anterior-and-posterior-fixation-improve-surgical-outcome
#2
Tsia-Shu Lo, Ma Clarissa Uy-Patrimonio, Wu-Chiao Hsieh, Ju-Chun Yang, Shih Yin Huang, Sandy Chua
INTRODUCTION AND HYPOTHESIS: Uterine preservation in uterine prolapse is an option for young patients. We hypothesized that sacrospinous hysteropexy (SSH) with anchorage to both the anterior and posterior cervix (SSH-ap) would have a better outcome than SSH with anchorage to the posterior cervix only (SSH-p). METHODS: This was a retrospective study including 75 patients who underwent SSH at Chang Gung Memorial Hospital between March 2008 and August 2013. Five were excluded due to incomplete data...
October 2, 2017: International Urogynecology Journal
https://www.readbyqxmd.com/read/28955364/identification-and-functional-annotation-of-genes-differentially-expressed-in-the-reproductive-tissues-of-the-olive-tree-olea-europaea-l-through-the-generation-of-subtractive-libraries
#3
Adoración Zafra, Rosario Carmona, José A Traverso, John T Hancock, Maria H S Goldman, M Gonzalo Claros, Simon J Hiscock, Juan D Alche
The olive tree is a crop of high socio-economical importance in the Mediterranean area. Sexual reproduction in this plant is an essential process, which determines the yield. Successful fertilization is mainly favored and sometimes needed of the presence of pollen grains from a different cultivar as the olive seizes a self-incompatibility system allegedly determined of the sporophytic type. The purpose of the present study was to identify key gene products involved in the function of olive pollen and pistil, in order to help elucidate the events and signaling processes, which happen during the courtship, pollen grain germination, and fertilization in olive...
2017: Frontiers in Plant Science
https://www.readbyqxmd.com/read/28933514/hypothermia-for-traumatic-brain-injury
#4
REVIEW
Sharon R Lewis, David Jw Evans, Andrew R Butler, Oliver J Schofield-Robinson, Phil Alderson
BACKGROUND: Hypothermia has been used in the treatment of brain injury for many years. Encouraging results from small trials and laboratory studies led to renewed interest in the area and some larger trials. OBJECTIVES: To determine the effect of mild hypothermia for traumatic brain injury (TBI) on mortality, long-term functional outcomes and complications. SEARCH METHODS: We ran and incorporated studies from database searches to 21 March 2016...
September 21, 2017: Cochrane Database of Systematic Reviews
https://www.readbyqxmd.com/read/28920065/the-influence-of-sex-steroid-hormones-on-the-response-to-trauma-and-burn-injury
#5
REVIEW
K Al-Tarrah, N Moiemen, J M Lord
Trauma and related sequelae result in disturbance of homeostatic mechanisms frequently leading to cellular dysfunction and ultimately organ and system failure. Regardless of the type and severity of injury, gender dimorphism in outcomes following trauma have been reported, with females having lower mortality than males, suggesting that sex steroid hormones (SSH) play an important role in the response of body systems to trauma. In addition, several clinical and experimental studies have demonstrated the effects of SSH on the clinical course and outcomes following injury...
2017: Burns and trauma
https://www.readbyqxmd.com/read/28900911/the-stim-orai-pathway-regulation-of-stim-and-orai-by-thiol-modifications
#6
Barbara A Niemeyer
Cysteines are among the least abundant amino acids found in proteins. Due to their unique nucleophilic thiol group, they are able to undergo a broad range of chemical modifications besides their known role in disulfide formation, such as S-sulfenylation (-SOH), S-sulfinylation (-SO(2)H), S-sufonylation (-SO(3)H), S-glutathionylation (-SSG), and S-sulfhydration (-SSH), among others. These posttranslational modifications can be irreversible and act as transitional modifiers or as reversible on-off switches for the function of proteins...
2017: Advances in Experimental Medicine and Biology
https://www.readbyqxmd.com/read/28900562/a-biomechanical-comparison-among-three-kinds-of-rebound-type-jumps-in-female-collegiate-athletes
#7
Miki Nariai, Naruto Yoshida, Atsushi Imai, Kazumichi Ae, Ryo Ogaki, Hirokazu Suhara, Hitoshi Shiraki
BACKGROUND: Single-legged drop jumps (SDJ), single-legged repetitive jumps (SRJ), and single-legged side hops (SSH) are often used as plyometric training and functional performance tests. Differences in the kinetics and kinematic characteristics of lower extremity joints during these jumps are unclear. HYPOTHESIS/PURPOSE: The purpose of this study was to investigate the joint motion and mechanical work of the takeoff leg from foot contact to foot-off during SDJ, SRJ, and SSH in the sagittal and frontal planes in female athletes...
August 2017: International Journal of Sports Physical Therapy
https://www.readbyqxmd.com/read/28894912/optimisation-of-the-mr-protocol-in-pregnant-women-with-suspected-acute-appendicitis
#8
Ilah Shin, Yong Eun Chung, Chansik An, Hye Sun Lee, Honsoul Kim, Joon Seok Lim, Myeong-Jin Kim
PURPOSE: To investigate the optimal magnetic resonance (MR) imaging protocol in pregnant women suspected of having acute appendicitis. MATERIALS AND METHODS: One hundred and forty-six pregnant women with suspected appendicitis were included. MR images were reviewed by two radiologists in three separate sessions. In session 1, only axial single-shot turbo spin echo (SSH-TSE) T2-weighted images (WI) were included with other routine sequences. In sessions 2 and 3, coronal and sagittal T2WI were sequentially added...
September 11, 2017: European Radiology
https://www.readbyqxmd.com/read/28882801/effect-of-immune-gene-silencing-in-wssv-infected-tiger-shrimp-penaeus-monodon
#9
M S Shekhar, A Gomathi, N K Dubey, K Vinaya Kumar, K K Vijayan
White spot syndrome virus, continues to cause huge economic loss to aquaculture industry. In the absence of effective therapeutics to control WSSV, it is important to understand the host pathogen interaction at the molecular level. Suppression subtractive hybridization (SSH) cDNA library was constructed which led to identification of several differentially expressed genes in response to WSSV infection in Penaeus monodon. The genes expressed in SSH cDNA library of shrimp gill and gut tissues belonged to a wide range of biological functions...
September 5, 2017: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/28874874/3-mercaptopyruvate-sulfurtransferase-produces-potential-redox-regulators-cysteine-and-glutathione-persulfide-cys-ssh-and-gssh-together-with-signaling-molecules-h2s2-h2s3-and-h2s
#10
Yuka Kimura, Shin Koike, Norihiro Shibuya, David Lefer, Yuki Ogasawara, Hideo Kimura
Cysteine-persulfide (Cys-SSH) is a cysteine whose sulfhydryl group is covalently bound to sulfur (sulfane sulfur). Cys-SSH and its glutathione (GSH) counterpart (GSSH) have been recognized as redox regulators, some of which were previously ascribed to cysteine and GSH. However, the production of Cys-SSH and GSSH is not well understood. Here, we show that 3-mercaptopyruvate sulfurtransferase (3MST) produces Cys-SSH and GSSH together with the potential signaling molecules hydrogen per- and tri-sulfide (H2S2 and H2S3)...
September 5, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28856827/transcriptomic-and-physiological-analyses-of-the-dinoflagellate-karenia-mikimotoi-reveal-non-alkaline-phosphatase-based-molecular-machinery-of-atp-utilization
#11
Hao Luo, Xin Lin, Ling Li, Lingxiao Lin, Chao Zhang, Senjie Lin
The ability to utilize dissolved organic phosphorus (DOP) is important for phytoplankton to survive the scarcity of dissolved inorganic phosphorus (DIP), and alkaline phosphatase (AP) has been the major research focus as a facilitating mechanism. Here we employed a unique molecular ecological approach and conducted a broader search for underpinning molecular mechanisms of adenosine triphosphate (ATP) utilization. Cultures of the dinoflagellate Karenia mikimotoi were set up in L1 medium (+P), DIP-depleted L1 medium (-P), and ATP-replacing-DIP medium (ATP)...
August 30, 2017: Environmental Microbiology
https://www.readbyqxmd.com/read/28822827/host-genes-regulate-transcription-of-sperm-introduced-hepatitis-b-virus-genes-in-embryo
#12
Ying Zhong, Dong-Ling Liu, Mohamed Morsi M Ahmed, Peng-Hao Li, Xiao-Ling Zhou, Qing-Dong Xie, Xiao-Qing Xu, Ting-Ting Han, Zhi-Wei Hou, Chen-Yao Zhong, Ji-Hua Huang, Fei Zeng, Tian-Hua Huang
Hepatitis B virus (HBV) can invade the male germline, and sperm-introduced HBV genes could be transcribed in embryo. This study was to explore whether viral gene transcription is regulated by host genes. Embryos were produced by in vitro fertilization of hamster oocytes with human sperm containing the HBV genome. Total RNA extracted from test and control embryos were subjected to SMART-PCR, SSH, microarray hybridization, sequencing and BLAST analysis. Twenty-nine sequences showing significant identity to five human gene families were identified, with CSH2, EIF4G2, PCBD2, PSG4 and TTN selected to represent target genes...
August 16, 2017: Reproductive Toxicology
https://www.readbyqxmd.com/read/28811037/effects-of-metal-contamination-on-the-gene-expression-profile-of-two-benthic-species-cerastoderma-edule-and-ruditapes-philippinarum
#13
Raquel Rodrigues, Joana Lourenço, Patrícia Pereira, Susana Carvalho, Sonia Mendo
This study aimed to identify new biomarkers for metal exposure in two bivalve species. Suppressive Subtractive Hybridization (SSH) was employed to evaluate the transcriptomic response of Cerastoderma edule and Ruditapes philippinarum to metal pollution. Protein synthesis and catalytic activity were the most affected metabolic processes in C. edule and R. philippinarum, respectively. Also, different genes responded to the effect of contamination in each species. The different response observed in both species reinforces the importance of including more than one bioindicator species in risk assessment studies...
August 12, 2017: Marine Pollution Bulletin
https://www.readbyqxmd.com/read/28755722/molecular-mechanism-of-glucose-6-phosphate-utilization-in-the-dinoflagellate-karenia-mikimotoi
#14
Chao Zhang, Hao Luo, Liangmin Huang, Senjie Lin
Phosphorus (P) is an essential nutrient for marine phytoplankton as for other living organisms, and the preferred form, dissolved inorganic phosphate (DIP), is often quickly depleted in the sunlit layer of the ocean. Phytoplankton have developed mechanisms to utilize organic forms of P (DOP). Hydrolysis of DOP to release DIP by alkaline phosphatase is believed to be the most common mechanism of DOP utilization. Little effort has been made, however, to understand other potential molecular mechanisms of utilizing different types of DOP...
July 2017: Harmful Algae
https://www.readbyqxmd.com/read/28725014/su-schrieffer-heeger-chain-with-one-pair-of-formula-see-text-symmetric-defects
#15
L Jin, P Wang, Z Song
The topologically nontrivial edge states induce [Formula: see text] transition in Su-Schrieffer-Heeger (SSH) chain with one pair of gain and loss at boundaries. In this study, we investigated a pair of [Formula: see text]-symmetric defects located inside the SSH chain, in particular, the defects locations are at the chain centre. The [Formula: see text] symmetry breaking of the bound states leads to the [Formula: see text] transition, the [Formula: see text]-symmetric phases and the localized states were studied...
July 19, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28724611/tumor-necrosis-factor-alpha-kidney-function-and-hypertension
#16
Eamonn Mehaffey, Dewan Syed Abdul Majid
Hypertension is considered to be a low-grade inflammatory condition characterized by the presence of various pro-inflammatory cytokines. The inflammatory cytokine Tumor Necrosis Factor-alpha (TNF-α) is a constituent of the pro-inflammatory environment that is associated with salt-sensitive hypertension (SSH) and related renal injury. Elevated angiotensin II (AngII) and other factors such as oxidative stress conditions promote TNF-α formation. Many recent studies have provided evidence that TNF-α exerts a direct renal action by regulating hemodynamic and excretory function in the kidney...
July 19, 2017: American Journal of Physiology. Renal Physiology
https://www.readbyqxmd.com/read/28654760/gametocidal-genes-of-aegilops-segregation-distorters-in-wheat-aegilops-wide-hybridization
#17
M Niranjana
Aegilops is a genus belonging to the family Poaceace, which have played an indispensible role in the evolution of bread wheat and continues to do so by transferring genes by wide hybridization. Being the secondary gene pool of wheat, gene transfer from Aegilops poses difficulties and segregation distortion is common. Gametocidal genes are the most well characterized class of segregation distorters reported in interspecific crosses of wheat with Aegilops. These "selfish" genetic elements ensure their preferential transmission to progeny at the cost of gametes lacking them without providing any phenotypic benefits to the plant, thereby causing a proportional reduction in fertility...
June 27, 2017: Genome Génome / Conseil National de Recherches Canada
https://www.readbyqxmd.com/read/28619100/cyclophilin-b-facilitates-the-replication-of-orf-virus
#18
Kui Zhao, Jida Li, Wenqi He, Deguang Song, Ximu Zhang, Di Zhang, Yanlong Zhou, Feng Gao
BACKGROUND: Viruses interact with host cellular factors to construct a more favourable environment for their efficient replication. Expression of cyclophilin B (CypB), a cellular peptidyl-prolyl cis-trans isomerase (PPIase), was found to be significantly up-regulated. Recently, a number of studies have shown that CypB is important in the replication of several viruses, including Japanese encephalitis virus (JEV), hepatitis C virus (HCV) and human papillomavirus type 16 (HPV 16). However, the function of cellular CypB in ORFV replication has not yet been explored...
June 15, 2017: Virology Journal
https://www.readbyqxmd.com/read/28606083/the-spiritual-history-in-outpatient-practice-attitudes-and-practices-of-health-professionals-in-the-adventist-health-system
#19
Harold G Koenig, Kathleen Perno, Ted Hamilton
BACKGROUND: A screening spiritual history (SSH) is how health professionals (HP) identify patients' spiritual values, beliefs and preferences (VBPs) in the outpatient setting. We report on attitudes and practices of HPs in the largest Protestant health system in the U.S., the Adventist Health System (AHS). METHOD: Physicians or mid-level practitioners (N = 1082) in AHS-affiliated practices were approached and 513 (47%) agreed to participate. Participants were asked to identify a "spiritual care coordinator" (nurse/staff) and complete a questionnaire that assessed demographics, practice characteristics, religious involvement, and attitudes/practices concerning the SSH...
June 12, 2017: BMC Medical Education
https://www.readbyqxmd.com/read/28596044/incidence-recurrence-and-risk-factors-of-hernias-following-stoma-reversal
#20
Brad S Oriel, Qi Chen, Kamal M F Itani
BACKGROUND: To determine the incidence and risk factors for stoma site (SSH) and incisional (IH) hernias following stoma reversal as well as their recurrence following repair. METHODS: A cohort of VA Surgical Quality Improvement Program patients undergoing stoma reversal from 2002 to 2014 were evaluated at a single institution. Variables were selected a priori and evaluated by univariate analyses. RESULTS: Of 114 stoma reversals, 63 utilized a midline approach...
August 2017: American Journal of Surgery
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