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https://www.readbyqxmd.com/read/28473212/hepatocellular-adenoma-in-a-european-flatfish-limanda-limanda-genetic-alterations-in-laser-capture-micro-dissected-tissue-and-global-transcriptomic-approach
#1
Adélaïde Lerebours, Emma Chapman, Brett P Lyons, John P Bignell, Grant D Stentiford, Jeanette M Rotchell
Liver tumours in flatfish have been diagnosed using histopathology for decades to monitor the impacts of marine pollution. Here we describe the application of specific gene (retinoblastoma, Rb) profiling in laser capture micro-dissected samples, and a suppression subtractive hybridization (SSH) approach to isolate differentially expressed genes in hepatocellular adenoma (HCA) samples from dab, Limanda limanda. The Rb profiles from apparently normal and HCA micro-dissected samples of fish from the North Sea showed no significant difference, and genotypic heterogeneity within defined histological phenotypes was observed...
May 1, 2017: Marine Pollution Bulletin
https://www.readbyqxmd.com/read/28455764/heterologous-expression-of-three-camellia-sinensis-small-heat-shock-protein-genes-confers-temperature-stress-tolerance-in-yeast-and-arabidopsis-thaliana
#2
Mingle Wang, Zhongwei Zou, Qinghui Li, Huahong Xin, Xujun Zhu, Xuan Chen, Xinghui Li
CsHSP17.7, CsHSP18.1, and CsHSP21.8 expressions are induced by heat and cold stresses, and CsHSP overexpression confers tolerance to heat and cold stresses in transgenic Pichia pastoris and Arabidopsis thaliana. Small heat shock proteins (sHSPs) are crucial for protecting plants against biotic and abiotic stresses, especially heat stress. However, knowledge concerning the functions of Camellia sinensis sHSP in heat and cold stresses remains poorly understood. In this study, three C. sinensis sHSP genes (i.e...
April 28, 2017: Plant Cell Reports
https://www.readbyqxmd.com/read/28451253/reversible-ratiometric-detection-of-highly-reactive-hydropersulfides-using-a-fret-based-dual-emission-fluorescent-probe
#3
Ryosuke Kawagoe, Ippei Takashima, Shohei Uchinomiya, Akio Ojida
Hydropersulfide (R-SSH) is an important class of reactive sulfur species (RSS) involved in a variety of physiological processes in mammals. A fluorescent probe capable of real-time detection of hydropersulfide levels in living cells would be a versatile tool to elucidate its roles in cell signalling and redox homeostasis. In this paper, we report a ratiometric fluorescent probe for hydropersulfide sensing, based on a fluorescence resonance energy transfer (FRET) mechanism. This sensing mechanism involves a nucleophilic reaction of a hydropersulfide with the pyronine-unit of the probe, which modulates the intramolecular FRET efficiency to induce a dual-emission change...
February 1, 2017: Chemical Science
https://www.readbyqxmd.com/read/28448540/bruchid-egg-induced-transcript-dynamics-in-developing-seeds-of-black-gram-vigna-mungo
#4
Indrani K Baruah, Debashis Panda, Jagadale M V, Deba Jit Das, Sumita Acharjee, Priyabrata Sen, Bidyut Kumar Sarmah
Black gram (Vigna mungo) seeds are a rich source of digestible proteins, however, during storage these seeds are severely damaged by bruchids (Callosobruchus spp.), reducing seed quality and yield losses. Most of the cultivated genotypes of black gram are susceptible to bruchids, however, few tolerant genotypes have also been identified but the mechanism of tolerance is poorly understood. We employed Suppression Subtractive Hybridization (SSH) to identify specifically, but rarely expressed bruchid egg induced genes in black gram...
2017: PloS One
https://www.readbyqxmd.com/read/28446754/phonon-mediated-repulsion-sharp-transitions-and-quasi-self-trapping-in-the-extended-peierls-hubbard-model
#5
J Sous, M Chakraborty, C P J Adolphs, R V Krems, M Berciu
We study two identical fermions, or two hard-core bosons, in an infinite chain and coupled to phonons by interactions that modulate their hopping as described by the Peierls/Su-Schrieffer-Heeger (SSH) model. We show that exchange of phonons generates effective nearest-neighbor repulsion between particles and also gives rise to interactions that move the pair as a whole. The two-polaron phase diagram exhibits two sharp transitions, leading to light dimers at strong coupling and the flattening of the dimer dispersion at some critical values of the parameters...
April 26, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28445253/micro-rna-screening-and-prediction-model-construction-for-diagnosis-of-salt-sensitive-essential-hypertension
#6
Han Qi, Zheng Liu, Bin Liu, Han Cao, Weiping Sun, Yuxiang Yan, Ling Zhang
Commonly used tests for diagnosis of salt-sensitive hypertension (SSH) are complex and time-consuming, so new methods are required. Many studies have demonstrated roles for miRNAs in hypertension; however, the diagnostic value of miRNAs has yet to be determined for human SSH. In this study, we examined miRNA expression profiles by initial high-throughput miRNA sequencing of samples from patients with salt-sensitive and salt-resistant hypertension (SSH and SRH, respectively; n = 6, both groups), followed by validation by quantitative real-time polymerase chain reaction (qRT-PCR) in a larger cohort (n = 91)...
April 2017: Medicine (Baltimore)
https://www.readbyqxmd.com/read/28444590/transcripts-of-pectin-degrading-enzymes-and-isolation-of-complete-cdna-sequence-of-a-pectate-lyase-gene-induced-by-coffee-white-stem-borer-xylotrechus-quadripes-in-the-bark-tissue-of-coffea-canephora-robusta-coffee
#7
Kosaraju Bharathi, P Santosh, H L Sreenath
Of the two commercially cultivated coffee (Coffea) species, C. arabica (arabica) is highly susceptible and C. canephora (robusta) is highly resistant to the insect pest Xylotrechus quadripes (Coleoptera: Cerambycidae), commonly known as coffee white stem borer (CWSB). We constructed a forward-subtracted cDNA library by Suppression Subtractive Hybridization (SSH) from robusta bark tissue for profiling genes induced by CWSB infestation. Among the 265 unigenes of the SSH EST library, 7 unigenes (5 contigs and 2 singletons) matching different pectin-degrading enzymes were discovered...
May 2017: 3 Biotech
https://www.readbyqxmd.com/read/28443673/fluorescent-probes-and-selective-inhibitors-for-biological-studies-of-hydrogen-sulfide-and-polysulfide-mediated-signaling
#8
Yoko Takano, Honami Echizen, Kenjiro Hanaoka
SIGNIFICANCE: Hydrogen sulfide (H2S) plays roles in many physiological processes, including relaxation of vascular smooth muscles, mediation of neurotransmission, inhibition of insulin signaling, and regulation of inflammation. Also, hydropersulfide (R-S-SH) and polysulfide (-S-Sn-S-) have recently been identified as reactive sulfur species (RSS) that regulate the bioactivities of multiple proteins via S-sulfhydration of cysteine residues (protein Cys-SSH) and show cytoprotection. Chemical tools such as fluorescent probes and selective inhibitors are needed to establish in detail the physiological roles of H2S and polysulfide...
May 18, 2017: Antioxidants & Redox Signaling
https://www.readbyqxmd.com/read/28428796/expression-profiling-coupled-with-in-silico-mapping-identifies-candidate-genes-for-reducing-aflatoxin-accumulation-in-maize
#9
Ramesh Dhakal, Chenglin Chai, Ratna Karan, Gary L Windham, William P Williams, Prasanta K Subudhi
Aflatoxin, produced by Aspergillus flavus, is hazardous to health of humans and livestock. The lack of information about large effect QTL for resistance to aflatoxin accumulation is a major obstacle to employ marker-assisted selection for maize improvement. The understanding of resistance mechanisms of the host plant and the associated genes is necessary for improving resistance to A. flavus infection. A suppression subtraction hybridization (SSH) cDNA library was made using the developing kernels of Mp715 (resistant inbred) and B73 (susceptible inbred) and 480 randomly selected cDNA clones were sequenced to identify differentially expressed genes (DEGs) in response to A...
2017: Frontiers in Plant Science
https://www.readbyqxmd.com/read/28420190/overexpression-of-s-adenosyl-l-methionine-synthetase-2-from-sugar-beet-m14-increased-arabidopsis-tolerance-to-salt-and-oxidative-stress
#10
Chunquan Ma, Yuguang Wang, Dan Gu, Jingdong Nan, Sixue Chen, Haiying Li
The sugar beet monosomic addition line M14 is a unique germplasm that contains genetic materials from Beta vulgaris L. and Beta corolliflora Zoss, and shows tolerance to salt stress. Our study focuses on exploring the molecular mechanism of the salt tolerance of the sugar beet M14. In order to identify differentially expressed genes in M14 under salt stress, a subtractive cDNA library was generated by suppression subtractive hybridization (SSH). A total of 36 unique sequences were identified in the library and their putative functions were analyzed...
April 18, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/28404756/infusion-compatible-antibiotic-formulations-for-rapid-administration-to-improve-outcomes-in-cancer-outpatients-with-severe-sepsis-and-septic-shock-the-sepsis-stat-pack
#11
Jason D Goldman, Amelia Gallaher, Rupali Jain, Zach Stednick, Manoj Menon, Michael J Boeckh, Paul S Pottinger, Stephen M Schwartz, Corey Casper
Background: Patients with cancer are at high risk for severe sepsis and septic shock (SS/SSh), and a delay in receiving effective antibiotics is strongly associated with mortality. Delays are due to logistics of clinic flow and drug delivery. In an era of increasing antimicrobial resistance, combination therapy may be superior to monotherapy for patients with SS/SSh. Patients and Methods: At the Seattle Cancer Care Alliance, we implemented the Sepsis STAT Pack (SSP) program to simplify timely and effective provision of empiric antibiotics and other resuscitative care to outpatients with cancer with suspected SS/SSh before hospitalization...
April 2017: Journal of the National Comprehensive Cancer Network: JNCCN
https://www.readbyqxmd.com/read/28394844/implant-material-type-of-fixation-at-the-shaft-and-position-of-plate-modify-biomechanics-of-distal-femur-plate-osteosynthesis
#12
Utku Kandemir, Peter Augat, Stefanie Konowalczyk Dipl-Ing, Felix Wipf Dipl-Ing, Geert von Oldenburg Dipl-Ing, Ulf Schmidt
OBJECTIVES: The purpose of this study is to investigate whether 1. the type of fixation at the shaft (hybrid vs. locking), 2. the position of the plate (offset vs. contact) and 3. the implant material has a significant effect on a) construct stiffness and b) fatigue life in a distal femur extraarticular comminuted fracture model using the same design of distal femur periarticular locking plate. METHODS: An extraarticular severely comminuted distal femoral fracture pattern (AO/OTA 33-A3) was simulated using artificial bone substitutes...
April 7, 2017: Journal of Orthopaedic Trauma
https://www.readbyqxmd.com/read/28365205/articular-ultrasound-in-asymptomatic-volunteers-identification-of-the-worst-measures-of-synovial-hypertrophy-synovial-blood-flow-and-joint-damage-among-small-medium-%C3%A2-and-large-sized-joints
#13
Flávia Soares Machado, Jamil Natour, Rogerio Diniz Takahashi, Rita N V Furtado
Articular ultrasound of 6500 joint recesses was performed for the purpose of identifying which joint had the highest measurements among small-sized (SSJ), medium-sized (MSJ) and large-sized (LSJ) joints. Quantitative measurements of synovial hypertrophy (QSR) and semiquantitative measurements of synovial hypertrophy (SSH), power Doppler (SPD) and bone erosion (SBE) (score: 0-3) were made. Higher measurements (p < 0.01) of QSR were obtained in the second metatarsophalangeal joint (MTP), talonavicular joint, and hip...
June 2017: Ultrasound in Medicine & Biology
https://www.readbyqxmd.com/read/28360125/cellular-levels-of-grb2-and-cytoskeleton-stability-are-correlated-in-a-neurodegenerative-scenario
#14
Piyali Majumder, Kasturi Roy, Brijesh Kumar Singh, Nihar Ranjan Jana, Debashis Mukhopadhyay
Alzheimer's disease (AD) manifests as neuronal loss. On the premise of Grb2 overexpression in AD mouse brain and brain tissues of AD patients, our study primarily focuses on the stability of cytoskeletal proteins in the context of degenerative AD-like conditions. Two predominant molecular features of AD, extracellular accumulation of β-amyloid oligomers and intracellular elevation of amyloid precursor protein intracellular domain levels, have been used to closely inspect the series of signalling events. In their presence, multiple signalling pathways involving ROCK and PAK1 proteins lead to disassembly of the cytoskeleton, and Grb2 partially counterbalances the cytoskeletal loss...
May 1, 2017: Disease Models & Mechanisms
https://www.readbyqxmd.com/read/28343634/identification-of-heavy-metal-pollutant-tolerance-associated-genes-in-avicennia-marina-forsk-by-suppression-subtractive-hybridization
#15
Jicheng Zhang, Jinfeng Yu, Hualong Hong, Jingchun Liu, Haoliang Lu, Chongling Yan
The halophytic Avicennia marina (Forsk.) is one of the pioneer mangroves along the south coast of China. It is an appropriate material for understanding molecular mechanisms of heavy metal tolerance in mangrove plants. A forward and a reverse cDNA library was constructed by PCR-based suppressive subtractive hybridization (SSH) to isolate these tolerance-associated genes from A. marina leaves. A total of 99 ESTs obtained from the forward and reverse libraries showed significant differential expressions. Twenty-nine genes selected by SSH were studied by real-time PCR in order to analyze their expression level...
March 23, 2017: Marine Pollution Bulletin
https://www.readbyqxmd.com/read/28342115/ssh-versus-tse-sequence-protocol-in-rapid-mr-examination-of-pediatric-patients-with-programmable-drainage-system
#16
Eva Brichtová, J Šenkyřík
PURPOSE: A low radiation burden is essential during diagnostic procedures in pediatric patients due to their high tissue sensitivity. Using MR examination instead of the routinely used CT reduces the radiation exposure and the risk of adverse stochastic effects. Our retrospective study evaluated the possibility of using ultrafast single-shot (SSh) sequences and turbo spin echo (TSE) sequences in rapid MR brain imaging in pediatric patients with hydrocephalus and a programmable ventriculoperitoneal drainage system...
March 24, 2017: Child's Nervous System: ChNS: Official Journal of the International Society for Pediatric Neurosurgery
https://www.readbyqxmd.com/read/28280499/deep-sequencing-of-suppression-subtractive-hybridisation-drought-and-recovery-libraries-of-the-non-model-crop-trifolium-repens-l
#17
Maciej Bisaga, Matthew Lowe, Matthew Hegarty, Michael Abberton, Adriana Ravagnani
White clover is a short-lived perennial whose persistence is greatly affected by abiotic stresses, particularly drought. The aim of this work was to characterize its molecular response to water deficit and recovery following re-hydration to identify targets for the breeding of tolerant varieties. We created a white clover reference transcriptome of 16,193 contigs by deep sequencing (mean base coverage 387x) four Suppression Subtractive Hybridization (SSH) libraries (a forward and a reverse library for each treatment) constructed from young leaf tissue of white clover at the onset of the response to drought and recovery...
2017: Frontiers in Plant Science
https://www.readbyqxmd.com/read/28274853/identification-of-sennoside-a-as-a-novel-inhibitor-of-the-slingshot-ssh-family-proteins-related-to-cancer-metastasis
#18
Seon Young Lee, Wooil Kim, Young Geun Lee, Hyo Jin Kang, Sang-Hyun Lee, Sun Young Park, Jeong-Ki Min, Sang-Rae Lee, Sang J Chung
Phospho-cofilin (p-cofilin), which has a phosphate group on Ser-3, is involved in actin polymerization. Its dephosphorylated form promotes filopodia formation and cell migration by enhancing actin depolymerization. Protein phosphatase slingshot homologs (SSHs), known as dual-specificity phosphatases, catalyze hydrolytic removal of the Ser-3 phosphate group from phospho-cofilin. Aberrant SSH activity results in cancer metastasis, implicating SSHs as potential therapeutic targets for cancer metastasis. In this study, we screened 658 natural products purified from traditional oriental medicinal plants to identify three potent SSH inhibitors with submicromolar or single-digit micromolar Ki values: gossypol, hypericin, and sennoside A...
May 2017: Pharmacological Research: the Official Journal of the Italian Pharmacological Society
https://www.readbyqxmd.com/read/28225002/topologically-protected-localised-states-in-spin-chains
#19
Marta P Estarellas, Irene D'Amico, Timothy P Spiller
We consider spin chain families inspired by the Su, Schrieffer and Hegger (SSH) model. We demonstrate explicitly the topologically induced spatial localisation of quantum states in our systems. We present detailed investigations of the effects of random noise, showing that these topologically protected states are very robust against this type of perturbation. Systems with such topological robustness are clearly good candidates for quantum information tasks and we discuss some potential applications. Thus, we present interesting spin chain models which show promising applications for quantum devices...
February 22, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28219759/reevaluating-species-number-distribution-and-endemism-of-the-coral-genus-pocillopora-lamarck-1816-using-species-delimitation-methods-and-microsatellites
#20
P Gélin, B Postaire, C Fauvelot, H Magalon
Species delimitation methods based on genetic information, notably using single locus data, have been proposed as means of increasing the rate of biodiversity description, but can also be used to clarify complex taxonomies. In this study, we explore the species diversity within the cnidarian genus Pocillopora, widely distributed in the tropical belt of the Indo-Pacific Ocean. From 943 Pocillopora colonies sampled in the Western Indian Ocean, the Tropical Southwestern Pacific and Southeast Polynesia, representing a huge variety of morphotypes, we delineated Primary Species Hypotheses (PSH) applying the Automatic Barcode Gap Discovery method, the Poisson Tree Processes algorithm and the Generalized mixed Yule-coalescent model on two mitochondrial markers (Open Reading Frame and Dloop) and reconstructing a haploweb using one nuclear marker (Internal Transcribed Spacer 2)...
February 17, 2017: Molecular Phylogenetics and Evolution
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