Mengyu Liu, Tong Wang, Lu Wang, Hanjie Xiao, Jinhui Li, Chunhui Duan, Lijie Gao, Yueqin Liu, Hui Yan, Yingjie Zhang, Shoukun Ji
INTRODUCTION: This study aimed to investigate the digestive function, urea utilization ability, and bacterial composition changes in rumen microbiota under high urea (5% urea in diet) over 23 days of continuous batch culture in vitro . METHODS: The gas production, dry matter digestibility, and bacterial counts were determined for the continuously batch-cultured rumen fluid (CRF). The changes in fermentation parameters, NH3 -N utilization efficiency, and microbial taxa were analyzed in CRF and were compared with that of fresh rumen fluid (RF), frozen rumen fluid (FRF, frozen rumen fluid at -80°C for 1 month), and the mixed rumen fluid (MRF, 3/4 RF mixed with 1/4 CRF) with in vitro rumen fermentation...
2024: Frontiers in Microbiology