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https://www.readbyqxmd.com/read/29136849/simultaneous-determination-of-three-estrogens-in-human-saliva-without-derivatization-or-liquid-liquid-extraction-for-routine-testing-via-miniaturized-solid-phase-extraction-with-lc-ms-ms-detection
#1
Xiaoguang Sunny Li, Shu Li, Gottfried Kellermann
Accurate quantitation of estrogens (i.e, estrone (E1), estradiol (E2) and estriol (E3)) is valuable for clinical assessment of human health and disease. Alterations in estrogen levels have been implicated in numerous pathological conditions. However, inadequacies in sensitivity and specificity, cumbersome sample preparation and invasive specimen collection hamper the usability of available methods for clinical applications. Herein, a simple, rapid, highly sensitive and specific LC-MS/MS method was developed and validated for the simultaneous determination of three estrogens in human saliva providing a non-invasive alternative to conventional blood samples...
February 1, 2018: Talanta
https://www.readbyqxmd.com/read/29129624/women-s-preferences-for-men-s-beards-show-no-relation-to-their-ovarian-cycle-phase-and-sex-hormone-levels
#2
Barnaby J W Dixson, Anthony J Lee, Khandis R Blake, Grazyna Jasienska, Urszula M Marcinkowska
According to the ovulatory shift hypothesis, women's mate preferences for male morphology indicative of competitive ability, social dominance, and/or underlying health are strongest at the peri-ovulatory phase of the menstrual cycle. However, recent meta-analyses are divided on the robustness of such effects and the validity of the often-used indirect estimates of fertility and ovulation has been called into question in methodological studies. In the current study, we test whether women's preferences for men's beardedness, a cue of male sexual maturity, androgenic development and social dominance, are stronger at the peri-ovulatory phase of the menstrual cycle compared to during the early follicular or the luteal phase...
November 9, 2017: Hormones and Behavior
https://www.readbyqxmd.com/read/29105068/activation-of-plasmacytoid-dendritic-cells-by-apoptotic-particles-mechanism-for-the-loss-of-immunologic-tolerance-in-sj%C3%A3-gren-s-syndrome
#3
Mari Ainola, Pauliina Porola, Yuya Takakubo, Beata Przybyla, Vesa-Petteri Kouri, Tuomas Aleksi Tolvanen, Arno Hänninen, Dan Christian Nordström
Sjögren's syndrome (SS) is a common autoimmune disease targeting salivary and lacrimal glands. It is strongly female-dominant characterized by low estrogen levels combined with a local intracrine dihydrotestosterone defect. We hypothesized that these hormonal deficits lead to increased apoptosis of the epithelial cells and plasmacytoid dendritic cell (pDC) mediated pro-inflammatory host responses. Expression of Toll-like receptors (TLRs) 7 and 9 and cytokine profile was studied in pDCs treated with apoptotic particles collected in consecutive centrifugation steps of media from apoptotic cells...
November 4, 2017: Clinical and Experimental Immunology
https://www.readbyqxmd.com/read/29094961/developmental-differences-in-reward-sensitivity-and-sensation-seeking-in-adolescence-testing-sex-specific-associations-with-gonadal-hormones-and-pubertal-development
#4
K Paige Harden, Frank D Mann, Andrew D Grotzinger, Megan W Patterson, Laurence Steinberg, Jennifer L Tackett, Elliot M Tucker-Drob
Sensation seeking has been found to increase, on average, from childhood to adolescence. Developmental scientists have hypothesized that this change could be driven by the rise of gonadal hormones at puberty, which affect reward-related processing in the brain. In a large, age-heterogeneous, population-based sample of adolescents and young adults (N = 810; ages 13-20 years), we tested for sex-specific associations between age, self-reported pubertal development, gonadal hormones (estradiol and testosterone) as measured in saliva, reward sensitivity as measured by a multivariate battery of in-laboratory tasks (including the Iowa gambling task, balloon analogue risk task, and stoplight task), and self-reported sensation seeking...
November 2, 2017: Journal of Personality and Social Psychology
https://www.readbyqxmd.com/read/28905874/ovarian-hormones-and-transdermal-nicotine-administration-independently-and-synergistically-suppress-tobacco-withdrawal-symptoms-and-smoking-reinstatement-in-the-human-laboratory
#5
Raina D Pang, Madalyn M Liautaud, Matthew G Kirkpatrick, Jimi Huh, John Monterosso, Adam M Leventhal
Modeling intra-individual fluctuations in estradiol and progesterone may provide unique insight into the effects of ovarian hormones on the etiology and treatment of nicotine dependence. This randomized placebo-controlled laboratory study tested the independent and interactive effects of intra-individual ovarian hormone variation and nicotine on suppression of tobacco withdrawal symptoms and smoking behavior. Female smokers randomized to 21 mg nicotine (TNP; n=37) or placebo (PBO; n=43) transdermal patch following overnight abstinence completed three sessions occurring during hormonally distinct menstrual cycle phases...
September 14, 2017: Neuropsychopharmacology: Official Publication of the American College of Neuropsychopharmacology
https://www.readbyqxmd.com/read/28843511/the-relationship-between-reproductive-and-biochemical-ageing-at-the-time-of-the-menopausal-transition
#6
Annamaria Zsakai, Rita Sipos, Krisztina Takacs-Vellai, Attila Szabo, Eva B Bodzsar
The biochemical ageing status of women in the menopausal transition was studied using quantitative analysis of age- and autophagy-related gene activities (CDC42 and MAP1LC3 genes were selected as target genes). Free estradiol and progesterone levels in saliva were estimated. General linear models were used to determine the relationship between lifestyle, health status, socioeconomic factors and CDC42 and MAP1LC3 gene expression levels. Gene expression analysis revealed (1) an increasing expression of CDC42 gene after 45years in women, (2) expression level of CDC42 gene associated with menopausal status, (3) while endocrine status was found to associate with the expression of both of the studied age-related genes, (4) the "never used hormonal contraceptives" and "obese nutritional status" were the strongest factors for increased level of age-related gene expressions, and (5) changes in gene expression levels by ageing should be studied by considering not only chronological, but also biological ages...
August 24, 2017: Experimental Gerontology
https://www.readbyqxmd.com/read/28751855/interactive-effects-of-dopamine-baseline-levels-and-cycle-phase-on-executive-functions-the-role-of-progesterone
#7
Esmeralda Hidalgo-Lopez, Belinda Pletzer
Estradiol and progesterone levels vary along the menstrual cycle and have multiple neuroactive effects, including on the dopaminergic system. Dopamine relates to executive functions in an "inverted U-shaped" manner and its levels are increased by estradiol. Accordingly, dopamine dependent changes in executive functions along the menstrual cycle have been previously studied in the pre-ovulatory phase, when estradiol levels peak. Specifically it has been demonstrated that working memory is enhanced during the pre-ovulatory phase in women with low dopamine baseline levels, but impaired in women with high dopamine baseline levels...
2017: Frontiers in Neuroscience
https://www.readbyqxmd.com/read/28750061/endocrine-and-aggressive-responses-to-competition-are-moderated-by-contest-outcome-gender-individual-versus-team-competition-and-implicit-motives
#8
Jon K Oxford, Johanna M Tiedtke, Anna Ossmann, Dominik Özbe, Oliver C Schultheiss
This study examined hormonal responses to competition in relation to gender, social context, and implicit motives. Participants (N = 326) were randomly assigned to win or lose in a 10-round, virtual face-to-face competition, in same-sex individual- and team-competition contexts. Saliva samples, taken before and twice after the competition, were assayed for testosterone (T), estradiol (E), progesterone (P), and cortisol (C). Implicit needs for power (nPower) and affiliation (nAffiliation) were assessed with a picture-story exercise before the competition...
2017: PloS One
https://www.readbyqxmd.com/read/28712952/a-robust-high-throughput-fungal-biosensor-assay-for-the-detection-of-estrogen-activity
#9
Christoph Zutz, Karen Wagener, Desislava Yankova, Stefanie Eder, Erich Möstl, Marc Drillich, Kathrin Rychli, Martin Wagner, Joseph Strauss
Estrogenic active compounds are present in a variety of sources and may alter biological functions in vertebrates. Therefore, it is crucial to develop innovative analytical systems that allow us to screen a broad spectrum of matrices and deliver fast and reliable results. We present the adaptation and validation of a fungal biosensor for the detection of estrogen activity in cow derived samples and tested the clinical applicability for pregnancy diagnosis in 140 mares and 120 cows. As biosensor we used a previously engineered genetically modified strain of the filamentous fungus Aspergillus nidulans, which contains the human estrogen receptor alpha and a reporter construct, in which β-galactosidase gene expression is controlled by an estrogen-responsive-element...
October 2017: Steroids
https://www.readbyqxmd.com/read/28692609/human-semen-quality-sperm-dna-damage-and-the-level-of-reproductive-hormones-in-relation-to-urinary-concentrations-of-parabens
#10
Joanna Jurewicz, Michał Radwan, Bartosz Wielgomas, Emila Dziewirska, Anetta Karwacka, Anna Klimowska, Paweł Kałużny, Paweł Radwan, Michał Bochenek, Wojciech Hanke
OBJECTIVE: The aim of this study was to evaluate the association between environmental exposure to parabens and semen quality parameters [main semen parameters, computer-aided semen analysis (CASA parameters], sperm chromatin structure, and the level of reproductive hormones in men [follicle-stimulating hormone (FSH), testosterone, estradiol]. METHODS: Urine samples collected from 315 men who attended the infertility clinic for diagnostic purposes with normal semen concentration of 15 to 300 mln/mL were analyzed for five parabens concentrations using a validated gas chromatography ion-tap mass spectrometry method...
November 2017: Journal of Occupational and Environmental Medicine
https://www.readbyqxmd.com/read/28686893/a-relationship-in-adrenal-androgen-levels-between-mothers-and-their-children-from-a-dioxin-exposed-region-in-vietnam
#11
Le Thai Anh, Teruhiko Kido, Seijiro Honma, Ho Dung Manh, Ikue Koike, Yuko Oyama, Hoang Duc Phuc, Rie Okamoto, Hideaki Nakagawa, Shoji F Nakayama, Dang Duc Nhu, Nguyen Hung Minh, Ngo Van Toan, Le Ke Son
Over the past decades, southern Vietnam has been burdened by dioxins from contaminated herbicides sprayed during the Vietnam War. In a previous study, we found that dioxin exposure decreased levels of salivary dehydroepiandrosterone (DHEA), an adrenal androgen, in 3-year-old children. In present study, to assess the relationship between adrenal hormones disruption in lactating mothers and in children, we compared mother-child pairs from dioxin- and nondioxin-contaminated regions. In 2010 and 2011, mother-child pairs from a dioxin hotspot region (n=37) and a non-contaminated region (n=47) were recruited and donated breast milk and serum samples for dioxin and steroid hormones determination...
December 31, 2017: Science of the Total Environment
https://www.readbyqxmd.com/read/28659579/sex-hormones-gonadotropins-and-sex-hormone-binding-globulin-in-infants-fed-breast-milk-cow-milk-formula-or-soy-formula
#12
Xin Fang, Lei Wang, Chunhua Wu, Huijing Shi, Zhijun Zhou, Scott Montgomery, Yang Cao
Measurement of endogenous hormones in early life is important to investigate the effects of hormonally active environmental compounds. To assess the possible hormonal effects of different feeding regimens in different sample matrices of infants, 166 infants were enrolled from two U.S hospitals between 2006 and 2009. The children were classified into exclusive soy formula, cow milk formula or breast milk regimens. Urine, saliva and blood samples were collected over the first 12 months of life. Estradiol, estrone, testosterone, luteinizing hormone (LH), follicle-stimulating hormone (FSH) and sex hormone-binding globulin (SHBG) levels were measured in the three matrices...
June 28, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28570489/possible-hormone-predictors-of-physical-performance-in-adolescent-team-sport-athletes
#13
Alanna C Martin, Ian T Heazlewood, Cecilia M Kitic, Isabelle Lys, Liam Johnson
The research aim of this study was to determine possible hormone predictors of physical performance in adolescent team sport athletes. Saliva samples were collected immediately prior to performance testing sessions from 114 state squad athletes (77 male, 37 female) participating in either Australian football, basketball, hockey, or netball. Participants completed tests of aerobic and anaerobic capacity, agility, power and speed. Samples were collected over 22 months at quarterly, six-monthly and/or yearly intervals depending on the testing schedule of the athlete...
May 30, 2017: Journal of Strength and Conditioning Research
https://www.readbyqxmd.com/read/28511046/effects-of-stress-on-women-s-preference-for-male-facial-masculinity-and-their-endocrine-correlates
#14
Beate Ditzen, Simona Palm-Fischbacher, Lara Gossweiler, Livia Stucky, Ulrike Ehlert
Women's preferences for masculinity in men's faces seem to vary across the menstrual cycle and are assumed to be strongest around ovulation. A number of hormones have been proposed to underlie these subtle cyclic shifts. Furthermore, mating preferences are context-dependent, and stress has been found to alter mate choice, both in animals and humans. Currently, the effects of stress on women's preference for masculinity remain unknown. To examine the hormonal basis and the impact of stress on facial masculinity preference, we tested for within-subject changes in 52 healthy young women who underwent the Trier Social Stress Test (TSST) and the placebo-TSST in randomized order in the late follicular and mid-luteal phases of their menstrual cycle...
May 3, 2017: Psychoneuroendocrinology
https://www.readbyqxmd.com/read/28420859/ultrastructural-and-physico-chemical-characterization-of-saliva-during-menstrual-cycle-in-perspective-of-ovulation-in-human
#15
Ganesan Saibaba, Mahalingam Srinivasan, Archunan Priya Aarthy, Velliyangiri Silambarasan, Govindaraju Archunan
Human saliva is a potential diagnostic fluid and any alteration in body might be reflected in saliva so that saliva is considered as "mirror of the body". Variations in salivary hormone level, ultra structure, pH, flow rate, buffering capacity and electrolytes level are found during menstrual cycle in regard to ovulation. Thirty healthy volunteers were used for the assessment of physico-chemical changes in saliva. Reproductive cycle was categorized as pre-ovulation phase (5 to 12 days), ovulation phase (13 or 14 days) and post-ovulation phase (15 to 25 days) according to salivary arborization test and hormonal analysis...
May 30, 2017: Drug Discoveries & Therapeutics
https://www.readbyqxmd.com/read/28407517/social-status-strategy-in-early-adolescent-girls-testosterone-and-value-based-decision-making
#16
Stephanie L Cardoos, Ahna Ballonoff Suleiman, Megan Johnson, Wouter van den Bos, Stephen P Hinshaw, Ronald E Dahl
There has been strong interest, spanning several disciplines, in understanding adolescence as a developmental period of increased risk-taking behavior. Our goals focus on one line of investigation within this larger developmental risk framework. Specifically, we examined levels of pubertal hormones in girls in relation to their willingness to take greater financial risks to gain social status. To this end, we tested the hypothesis that higher levels of testosterone during the ages of pubertal maturation are associated with a greater willingness to sacrifice money for social admiration...
March 31, 2017: Psychoneuroendocrinology
https://www.readbyqxmd.com/read/28278220/placental-hormone-profiles-as-predictors-of-preterm-birth-in-twin-pregnancy-a-prospective-cohort-study
#17
Hui Lim, Sioned Powell, Helen C Mcnamara, A Forbes Howie, Ann Doust, Maria E Bowman, Roger Smith, Jane E Norman, Sarah J Stock
OBJECTIVE: The objective of the study was to analyse placental hormone profiles in twin pregnancies to determine if they could be used to predict preterm birth. STUDY DESIGN: Progesterone, estradiol, estriol and corticotropin-releasing hormone were measured using competitive immunoassay and radioimmunoassay in serum and saliva samples of 98 women with twin pregnancies,at 3 or more gestational timepoints. Hormone profiles throughout gestation were compared between very preterm (<34 weeks; n = 8), preterm (<37 weeks; n = 40) and term (37+ weeks; n = 50) deliveries...
2017: PloS One
https://www.readbyqxmd.com/read/28211029/influence-of-segmental-body-composition-and-adiposity-hormones-on-resting-metabolic-rate-and-substrate-utilization-in-overweight-and-obese-adults
#18
K R Hirsch, A E Smith-Ryan, M N M Blue, M G Mock, E T Trexler
PURPOSE: Low resting metabolic rate (RMR) and high carbohydrate reliance at rest are associated with weight gain, but are highly variable in obese individuals. This study determined the relationship of total and segmental body composition and adiposity hormones with RMR and respiratory exchange ratio (RER) in overweight and obese adults. METHODS: In 49 men (n = 23) and premenopausal women (n = 26) [mean ± SD; age = 35.0 ± 8.9 years; body mass index (BMI) = 33...
June 2017: Journal of Endocrinological Investigation
https://www.readbyqxmd.com/read/28199919/relationships-between-oral-muc1-expression-and-salivary-hormones-in-burning-mouth-syndrome
#19
Jeong-Hyun Kang, Yoon-Young Kim, Ji-Youn Chang, Hong-Seop Kho
OBJECTIVES: To investigate possible relationships among oral mucosal epithelial MUC1 expression, salivary female gonadal hormones and stress markers, and clinical characteristics in patients with burning mouth syndrome (BMS). DESIGN: Thirty post-menopausal female patients with BMS (60.0±5.0 years) were included. Clinical and psychological evaluations were performed and the expression level of oral mucosal epithelial MUC1 was analyzed. The levels of cortisol, dehydroepiandrosterone (DHEA), 17β-estradiol, progesterone, chromogranin A, and blood contamination were determined from unstimulated whole saliva (UWS) and stimulated whole saliva (SWS) samples...
February 4, 2017: Archives of Oral Biology
https://www.readbyqxmd.com/read/28064085/cortisol-awakening-response-is-blunted-and-pain-perception-is-increased-during-menses-in-cyclic-women
#20
Tuba Ozgocer, Cihat Ucar, Sedat Yildiz
BACKGROUND AND AIMS: The incidence of menstrual symptoms is reported to be as high as 90% in cyclic women. These symptoms, including anxiety and pain, might be associated with cortisol, as its receptors are widely distributed in the brain areas associated with behavior. Therefore, the current study aimed to assess the cortisol awakening response (CAR) throughout the menstrual cycle and correlate it with pain perception and trait anxiety. MATERIALS AND METHODS: CAR was assessed by measuring salivary cortisol at 0, 15, 30, and 60min following awakening in the same women (n=59, age 22...
December 21, 2016: Psychoneuroendocrinology
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