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https://www.readbyqxmd.com/read/28972598/evaluation-of-occupational-physical-load-during-6-month-international-crisis-management-operation
#1
Kai Pihlainen, Matti Santtila, Tommi Vasankari, Keijo Häkkinen, Heikki Kyröläinen
OBJECTIVES: Generally, operational military duties are associated with a variety of stressors, such as prolonged physical activity (PA). However, limited information is available on the occupational workload or changes in PA during international military operations. Thus, the aim of the study was to investigate the changes in body composition, stress biomarkers, PA, and heart rate (HR) responses of 79 male soldiers during a 6-month international crisis management operation. MATERIAL AND METHODS: Measurements were conducted 3 times in South-Lebanon during the operation...
October 2, 2017: International Journal of Occupational Medicine and Environmental Health
https://www.readbyqxmd.com/read/28956262/daily-salivary-cortisol-and-cortisone-rhythm-in-patients-with-adrenal-incidentaloma
#2
Filippo Ceccato, Mattia Barbot, Nora Albiger, Giorgia Antonelli, Marialuisa Zilio, Marco Todeschini, Daniela Regazzo, Mario Plebani, Carmelo Lacognata, Maurizio Iacobone, Franco Mantero, Marco Boscaro, Carla Scaroni
BACKGROUND AND AIM: Impaired cortisol rhythm is a characteristic feature of Cushing's Syndrome, nevertheless late night salivary cortisol (LNSC) is not suitable to detect subclinical hypercortisolism in patients with adrenal incidentaloma (AI). We studied daily salivary cortisol (F) and cortisone (E) rhythm in patients with AI. MATERIALS AND METHODS: Six saliva samples were collected from awakening to night in 106 patients with AI and 40 controls. F and E were measured with LC-MS/MS and daily F exposure was calculated with the area under the curve (AUC)...
September 27, 2017: Endocrine
https://www.readbyqxmd.com/read/28862713/meta-analysis-of-micrornas-as-potential-biomarkers-for-detecting-esophageal-carcinoma-in-asian-populations
#3
Mei Li, Fei Wu, Yu Ji, Lan Yang, Feng Li
BACKGROUND: An Increasing number of studies in the literature have shown that microRNAs (miRNAs) can be used as early diagnostic markers for esophageal carcinoma (EC), but their conclusions remain controversial. Hence, we performed this meta-analysis to evaluate the diagnostic accuracy of using miRNAs in EC and to provide an experimental basis for early diagnosis of the disease. METHODS: This meta-analysis included 39 Asian studies from 18 articles, which covered 3,708 EC patients and 2,689 healthy controls...
August 28, 2017: International Journal of Biological Markers
https://www.readbyqxmd.com/read/28782189/epidermal-growth-factor-receptor-detection-in-serum-and-saliva-as-a-diagnostic-and-prognostic-tool-in-oral-cancer
#4
Laura Zanotti, Alberto Paderno, Cesare Piazza, Eleonora Pagan, Eliana Bignotti, Chiara Romani, Elisabetta Bandiera, Stefano Calza, Francesca Del Bon, Piero Nicolai, Antonella Ravaggi
OBJECTIVES/HYPOTHESIS: Epidermal growth factor receptor (EGFR) is a type I transmembrane glycoprotein that is overexpressed in a wide variety of malignancies, including oral squamous cell carcinoma (OSCC). Our objective was to assess the EGFR diagnostic and prognostic value in OSCC by investigating its expression in serum and saliva of patients in comparison with healthy subjects. STUDY DESIGN: Prospective case-control study. METHODS: Serum and saliva samples were collected from a cohort of 63 treatment-naïve OSCC patients before surgery and a matched group of 60 healthy subjects...
November 2017: Laryngoscope
https://www.readbyqxmd.com/read/28691471/salivary-glucose-as-a-metabolic-control-marker-in-patients-with-type-2-diabetes
#5
E Carramolino-Cuéllar, D Lauritano, F Carinci, J Silvestre-Rangil, C Bañuls-Morant, F J Silvestre, A Hernández-Mijares
A study was made of the correlation between the serum and salivary glucose levels in healthy subjects and in patients with type 2 diabetes, in order to establish the validity of salivary glucose determination in monitoring glycemia. Ninety-seven subjects were included in the study: 47 diabetic patients and 46 controls, aged between 40- and 80-years-of-age. Venous blood and saliva samples were collected in both groups under fasting conditions and after administering a test meal (15% proteins, 55% carbohydrates and 30% lipids)...
April 2017: Journal of Biological Regulators and Homeostatic Agents
https://www.readbyqxmd.com/read/28670189/acth-cortisol-and-il-6-levels-in-athletes-following-magnesium-supplementation
#6
Gordana Dmitrašinović, Vesna Pešić, Dušanka Stanić, Bosiljka Plećaš-Solarović, Marijana Dajak, Svetlana Ignjatović
BACKGROUND: Physical exercise activates the hypothalamo-pituitary-adrenal (HPA) axis and induces the body's inflammatory response. Due to contemporary dietary habits and increased energy expenditure, athletes are susceptible to depletion of magnesium ions. The aim of our study was to investigate, through assessment of plasma ACTH, serum IL-6, and salivary/serum cortisol levels, if chronic magnesium supplementation might reduce damaging stress effects in amateur rugby players. METHODS: Rugby players (N=23) were randomly assigned to intervention and control group...
October 2016: Journal of Medical Biochemistry
https://www.readbyqxmd.com/read/28419494/increased-levels-of-soluble-cd163-in-periodontitis-patients
#7
Laurent Detzen, Steven C Y Chen, Bin Cheng, Panos N Papapanou, Evanthia Lalla
AIM: Soluble CD163 (sCD163) has been implicated as a new biomarker in inflammatory conditions. The aim of this cross-sectional study was to assess CD163 levels systemically and locally in patients with chronic periodontitis. METHODS: sCD163 levels were measured by ELISA in serum samples from 70 periodontitis and 70 periodontally healthy subjects, and in saliva samples in a subset of the population. Two gingival biopsies were harvested per subject from 20 periodontitis patients: one from a periodontally affected site, the other from a healthy site, and the relative expression of CD163 mRNA was assessed by real-time PCR...
April 17, 2017: Journal of Clinical Periodontology
https://www.readbyqxmd.com/read/28185876/associations-between-food-consumption-patterns-and-saliva-composition-specificities-of-eating-difficulties-children
#8
Martine Morzel, Caroline Truntzer, Eric Neyraud, Hélène Brignot, Patrick Ducoroy, Géraldine Lucchi, Cécile Canlet, Ségolène Gaillard, Florian Nicod, Sophie Nicklaus, Noël Peretti, Gilles Feron
Identifying objective markers of diet would be beneficial to research fields such as nutritional epidemiology. As a preliminary study on the validity of using saliva for this purpose, and in order to explore the relationship between saliva and diet, we focused on clearly contrasted groups of children: children with eating difficulties (ED) receiving at least 50% of their energy intake through artificial nutrition vs healthy controls (C). Saliva of ED and C children was analyzed by various methods (targeted biochemical analyses, 2-D electrophoresis coupled to MS, (1)H NMR) and their diet was characterized using food frequency questionnaires, considering 148 food items grouped into 13 categories...
February 6, 2017: Physiology & Behavior
https://www.readbyqxmd.com/read/28104429/using-lysine-adducts-of-human-serum-albumin-to-investigate-the-disposition-of-exogenous-formaldehyde-in-human-blood
#9
MULTICENTER STUDY
Luca G Regazzoni, Hasmik Grigoryan, Zhiying Ji, Xi Chen, Sarah I Daniels, Deyin Huang, Sylvia Sanchez, Naijun Tang, Fenna C M Sillé, Anthony T Iavarone, Evan R Williams, Luoping Zhang, Stephen M Rappaport
Formaldehyde is a human carcinogen that readily binds to nucleophiles, including proteins and DNA. To investigate whether exogenous formaldehyde produces adducts in extracellular fluids, we characterized modifications to human serum albumin (HSA) following incubation of whole blood, plasma, and saliva with formaldehyde at concentrations of 1, 10 and 100μM. The only HSA locus that showed the presence of formaldehyde modifications was Lys199. A N(6)-Lys adduct with added mass of 12Da, representing a putative intramolecular crosslink, was detected in biological fluids that had been incubated with formaldehyde but not in control fluids...
February 15, 2017: Toxicology Letters
https://www.readbyqxmd.com/read/27983800/dual-metal-centered-zirconium-organic-framework-a-metal-affinity-probe-for-highly-specific-interaction-with-phosphopeptides
#10
Jiaxi Peng, Hongyan Zhang, Xin Li, Shengju Liu, Xingyun Zhao, Jing Wu, Xiaohui Kang, Hongqiang Qin, Zaifa Pan, Ren'an Wu
The highly specific affinity between probes and phosphopeptides is the fundamental interaction for selective identification of phosphoproteomes that uncover the mechanisms of signal transduction, cell cycle, enzymatic regulation, and gene expression in biological systems. In this study, a metal-affinity probe possessing both interactions of metal oxide affinity chromatography (MOAC) and immobilized metal ion affinity chromatography (IMAC) was facilely prepared by immobilizing zirconium(IV) on a zirconium-organic framework of UiO-66-NH2, which holds dual-metal centers of not only the inherent Zr-O cluster but also the immobilized Zr(IV) center...
December 28, 2016: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/27933485/fluid-based-biomarkers-for-amyotrophic-lateral-sclerosis
#11
REVIEW
Lucas T Vu, Robert Bowser
Amyotrophic lateral sclerosis (ALS) is a highly heterogeneous disease with no effective treatment. Drug development has been hampered by the lack of biomarkers that aid in early diagnosis, demonstrate target engagement, monitor disease progression, and can serve as surrogate endpoints to assess the efficacy of treatments. Fluid-based biomarkers may potentially address these issues. An ideal biomarker should exhibit high specificity and sensitivity for distinguishing ALS from control (appropriate disease mimics and other neurologic diseases) populations and monitor disease progression within individual patients...
January 2017: Neurotherapeutics: the Journal of the American Society for Experimental NeuroTherapeutics
https://www.readbyqxmd.com/read/27898397/multicenter-performance-evaluation-of-a-second-generation-cortisol-assay
#12
Michael Vogeser, Jürgen Kratzsch, Yoon Ju Bae, Mathias Bruegel, Uta Ceglarek, Tom Fiers, Alexander Gaudl, Hedwig Kurka, Christoph Milczynski, Cristina Prat Knoll, Anna C Suhr, Daniel Teupser, Ingrid Zahn, Richard E Ostlund
BACKGROUND: Untreated disorders of the adrenocortical system, such as Cushing's or Addison's disease, can be fatal, and accurate quantification of a patient's cortisol levels is vital for diagnosis. The objective of this study was to assess the analytical performance of a new fully-automated Elecsys® Cortisol II assay (second generation) to measure cortisol levels in serum and saliva. METHODS: Four European investigational sites assessed the intermediate precision and reproducibility of the Cortisol II assay (Roche Diagnostics) under routine conditions...
May 1, 2017: Clinical Chemistry and Laboratory Medicine: CCLM
https://www.readbyqxmd.com/read/27749218/serum-calprotectin-is-a-biomarker-of-carotid-atherosclerosis-in-patients-with-primary-sj%C3%A3-gren-s-syndrome
#13
Gabriela M Balarini, Eliana Zandonade, Leandro Tanure, Gilda A Ferreira, Wildner M Sardenberg, Érica V Serrano, Cleia C Dias, Túlio P Navarro, Hilde H Nordal, Piotr M Mydel, Johan G Brun, Karl A Brokstad, Eva Gerdts, Roland Jonsson, Valéria Valim
OBJECTIVES: We aimed to identify the association of carotid atherosclerosis with the traditional risk factors, disease features, cytokine profile, and calprotectin in patients with primary Sjögren's syndrome (pSS). METHODS: 63 primary pSS patients and 63 age- and sex-matched healthy controls underwent carotid ultrasound, clinical and laboratory examination. The presence of carotid plaques was taken as carotid atherosclerosis. The covariates of carotid atherosclerosis were identified in univariate and multivariate regressions...
November 2016: Clinical and Experimental Rheumatology
https://www.readbyqxmd.com/read/27547238/salivary-and-serum-il-10-tnf-%C3%AE-tgf-%C3%AE-vegf-levels-in-oropharyngeal-squamous-cell-carcinoma-and-correlation-with-hpv-and-ebv-infections
#14
Małgorzata Polz-Dacewicz, Małgorzata Strycharz-Dudziak, Jakub Dworzański, Agnieszka Stec, Joanna Kocot
BACKGROUND: Each year approximately 6,000 new cases of head and neck cancer are registered in Poland. Human papillomavirus (HPV) and Epstein-Barr virus (EBV) have been associated with tumour formation. Cytokines have been shown to play an important role both in inflammation and carcinogenesis and they can be detected in saliva and serum with ELISA assays. Salivary biomarkers may be used as markers of early cancer detection. The aim of this study was the analysis of the serum and salivary levels of IL-10, TNF-α, TGF-β and VEGF in patients with oropharyngeal cancer and in healthy individuals...
2016: Infectious Agents and Cancer
https://www.readbyqxmd.com/read/27484122/catestatin-vasostatin-cortisol-temperature-heart-rate-respiratory-rate-scores-of-the-short-form-of-the-glasgow-composite-measure-pain-scale-and-visual-analog-scale-for-stress-and-pain-behavior-in-dogs-before-and-after-ovariohysterectomy
#15
Thanikul Srithunyarat, Odd V Höglund, Ragnvi Hagman, Ulf Olsson, Mats Stridsberg, Anne-Sofie Lagerstedt, Ann Pettersson
BACKGROUND: The stress reaction induced by surgery and associated pain may be detrimental for patient recovery and should be minimized. The neuropeptide chromogranin A (CGA) has shown promise as a sensitive biomarker for stress in humans. Little is known about CGA and its derived peptides, catestatin (CST) and vasostatin (VS), in dogs undergoing surgery. The objectives of this study were to investigate and compare concentrations of CGA epitopes CST and VS, cortisol, body temperature, heart rate, respiratory rate, scores of the short form of the Glasgow composite measure pain scale (CMPS-SF) and visual analog scales (VAS) for stress and pain behavior in dogs before and after ovariohysterectomy...
August 2, 2016: BMC Research Notes
https://www.readbyqxmd.com/read/27120177/population-pharmacokinetics-of-prednisolone-in-relation-to-clinical-outcome-in-children-with-nephrotic-syndrome
#16
Nynke Teeninga, Zheng Guan, Jasper Stevens, Joana E Kist-van Holthe, Mariëtte T Ackermans, Albert J van der Heijden, Ron H N van Schaik, Teun van Gelder, Jeroen Nauta
BACKGROUND: The relapse frequency in children with nephrotic syndrome (NS) is highly variable despite standardized prednisolone treatment regimens. Existing evidence on the relationship between prednisolone pharmacokinetics (PK) and clinical response in children with NS is scarce and limited. The aim of this study was to develop a pediatric popPK model for prednisolone based on our previous model based on healthy adults using salivary measurements in children with NS and to correlate clinical outcome with between-subject variability in prednisolone exposure...
August 2016: Therapeutic Drug Monitoring
https://www.readbyqxmd.com/read/26969301/association-of-serum-electrolytes-and-smoking-with-salivary-gland-stone-formation
#17
A J Yiu, A Kalejaiye, R L Amdur, H N Todd Hesham, B C Bandyopadhyay
To further define potential factors that may contribute to stone formation in salivary glands (sialolithiasis), a retrospective chart review was performed of patients diagnosed with sialolithiasis between March 1, 1998 and February 29, 2012. Information on salivary gland stone number, location and size, medical history, medications, and serum electrolyte levels were collected. Associations between electrolyte levels and stone characteristics (such as stone number and size) were examined. Fifty-nine patients were identified; their median age was 58 years (range 25-89 years) and most were male (95%)...
June 2016: International Journal of Oral and Maxillofacial Surgery
https://www.readbyqxmd.com/read/26959798/metabolic-load-in-dairy-cows-kept-in-herbage-based-feeding-systems-and-suitability-of-potential-markers-for-compromised-well-being
#18
R S Zbinden, M Falk, A Münger, F Dohme-Meier, H A van Dorland, R M Bruckmaier, J J Gross
Herbage feeding with only little input of concentrates plays an important role in milk production in grassland dominated countries like Switzerland. The objective was to investigate the effects of a solely herbage-based diet and level of milk production on performance, and variables related to the metabolic, endocrine and inflammatory status to estimate the stress imposed on dairy cows. Twenty-five multiparous Holstein cows were divided into a control (C+, n = 13) and a treatment group (C-, n = 12), according to their previous lactation yield (4679-10 808 kg) from week 3 ante partum until week 8 post-partum (p...
March 9, 2016: Journal of Animal Physiology and Animal Nutrition
https://www.readbyqxmd.com/read/26855163/effect-of-sex-and-time-to-slaughter-transportation-and-lairage-duration-on-the-levels-of-cortisol-creatine-kinase-and-subsequent-relationship-with-pork-quality
#19
N Jama, V Maphosa, L C Hoffman, V Muchenje
The study determined the effect of sex and time to slaughter on cortisol and creatine kinase levels, and pork quality in commercial crossbred pigs. Saliva samples were before collected transportation, on arrival at the abattoir, and after a 20 hour lairage period. Cortisol levels from saliva (SC), serum (SeC) and urine (UC) were determined. Creatine kinase (CK) levels were determined from serum samples. Fifteen boars vs. 15 gilts were immediately slaughtered on arrival (SOA), and the other 15 boars vs. 15 gilts were rested for 20 h before slaughter...
June 2016: Meat Science
https://www.readbyqxmd.com/read/26805529/-the-clinical-effect-of-airway-pressure-release-ventilation-for-acute-lung-injury-acute-respiratory-distress-syndrome
#20
RANDOMIZED CONTROLLED TRIAL
Shaohua Song, Huiyu Tian, Xiufen Yang, Zhenjie Hu
OBJECTIVE: To evaluate the effect of airway pressure release ventilation (APRV) in patients with acute lung injury/acute respiratory distress syndrome (ALI/ARDS), to evaluate the extent of ventilator-induced lung injury (VILI), and to explore its possible mechanism. METHODS: A prospective study was conducted in the Department of Critical Care Medicine of the First Hospital of Hebei Medical University from December 2010 to February 2012. The patients with ALI/ARDS were enrolled...
January 2016: Zhonghua Wei Zhong Bing Ji Jiu Yi Xue
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