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https://www.readbyqxmd.com/read/28165444/correlation-between-cervical-infection-and-preterm-labor
#1
Larisa Mešić Ðogić, Nenad Lučić, Dragana Mićić, Feđa Omeragić, Enes Hodžić, Seid Fazlagić, Refka Kovač, Nevenka Pavlović
Aim To investigate a correlation between cervical canal infection and imminent preterm labor and to identify most frequent pathogens. Methods A prospective study was conducted in obstetrics/gynecology departments of Health Center and the University Clinical Center Tuzla, and General Hospital Tešanj (Bosnia and Herzegovina, B&H) between October 2013 and May 2014. An examined group included 50 healthy pregnant women with singleton pregnancy of the gestation age between the 28th and 37th week, with cervical changes that are related to imminent preterm labor...
February 1, 2017: Medicinski Glasnik
https://www.readbyqxmd.com/read/28152632/cervical-fluid-calreticulin-and-cathepsin-g-in-pregnancies-complicated-by-preterm-prelabor-rupture-of-membranes
#2
Ctirad Andrys, Ivana Musilova, Marcela Drahosova, Ondrej Soucek, Lenka Pliskova, Bo Jacobsson, Nanbert Zhong, Marian Kacerovsky
OBJECTIVE: The study aimed to determine the cervical calreticulin and cathepsin-G concentrations in pregnancies complicated by preterm prelabor rupture of membranes (PPROM) with respect to the presence of microbial invasion of the amniotic cavity (MIAC) and intra-amniotic inflammation (IAI). METHODS: Eighty women with singleton pregnancies complicated by PPROM were included in this study. Cervical and amniotic fluids were obtained at the time of admission, and concentrations of calreticulin and cathepsin-G in cervical fluid were determined using ELISA...
February 2, 2017: Journal of Maternal-fetal & Neonatal Medicine
https://www.readbyqxmd.com/read/28141596/comparison-of-mycoplasma-ies-mycofast-revolution-and-mycoplasma-ist2-to-detect-genital-mycoplasmas-in-clinical-samples
#3
Tiziana D'Inzeo, Giulia De Angelis, Barbara Fiori, Giulia Menchinelli, Flora Marzia Liotti, Grazia Angela Morandotti, Flavio De Maio, Domenico Nagel, Marco Antonaci, Maurizio Sanguinetti, Teresa Spanu
INTRODUCTION: Culture is regarded as the gold standard for the detection of genital mycoplasma in clinical samples. Commercially available diagnostic kits, based on liquid broth cultures, provide interesting alternatives to conventional culture. We assessed the laboratory performances of Mycoplasma IES (IES), the Mycofast Revolution (REV) and Mycoplasma IST 2 (IST2) compared to A7 agar plates for the detection of Ureaplasma urealyticum and Mycoplasma hominis in clinical samples. METHODOLOGY: From April to July 2013, endocervical or vaginal samples were collected from sexually active women with abnormal vaginal discharge...
January 30, 2017: Journal of Infection in Developing Countries
https://www.readbyqxmd.com/read/28129336/airway-microbial-community-turnover-differs-by-bpd-severity-in-ventilated-preterm-infants
#4
Brandie D Wagner, Marci K Sontag, J Kirk Harris, Joshua I Miller, Lindsey Morrow, Charles E Robertson, Mark Stephens, Brenda B Poindexter, Steven H Abman, Peter M Mourani
Preterm birth exposes the developing lung to an environment with direct exposure to bacteria, often facilitated by endotracheal intubation. Despite evidence linking bacterial infections to the pathogenesis of bronchopulmonary dysplasia (BPD), systematic studies of airway microbiota are limited. The objective was to identify specific patterns of the early respiratory tract microbiome from tracheal aspirates of mechanically ventilated preterm infants that are associated with the development and severity of BPD...
2017: PloS One
https://www.readbyqxmd.com/read/28112332/-molecular-detection-of-sexually-transmitted-agents-in-a-symptomatic-group-of-men-and-its-relationship-with-sexual-behavior
#5
Daniela León, Javier Retamal, Ramón Silva, Carmen Ili, Stephanie Mieville, Pablo Guzmán, Gastón Briceño, Priscilla Brebi
BACKGROUND: Sexually transmitted infections (STIs) affect sexual and reproductive health of millions of men. Pathogens such as human papillomavirus (HPV), herpes simplex virus type 1 and 2 (HSV-1 y HSV-2), Chlamydia trachomatis,Mycoplasmagenitalium,Mycoplasma hominis and Ureaplasma urealyticum are associated with STIs. AIM: To detect pathogens associated with STIs in symptomatic men and its relationship with sexual behavior. METHODOLOGY: DNA was obtained from exfoliated cells of penis from 20 symptomatic men...
October 2016: Revista Chilena de Infectología: órgano Oficial de la Sociedad Chilena de Infectología
https://www.readbyqxmd.com/read/28109354/ureaplasma-transmitted-from-donor-lungs-is-pathogenic-after-lung-transplantation
#6
Ramiro Fernandez, Amy Ratliff, Donna Crabb, Ken B Waites, Ankit Bharat
Hyperammonemia is a highly fatal syndrome in lung recipients that is usually refractory to medical therapy. We recently reported that infection by a Mollicute, Ureaplasma, is causative for hyperammonemia and can be successfully treated with antimicrobial agents. However, it remains unknown whether the pathogenic strain of Ureaplasma is donor or recipient derived. Here we provide evidence that donor-derived Ureaplasma infection can be pathogenic. As such, we uncover a previously unknown lethal donor-derived opportunistic infection in lung recipients...
February 2017: Annals of Thoracic Surgery
https://www.readbyqxmd.com/read/28099135/mycoplasma-ureaplasma-infection-in-pregnancy-to-screen-or-not-to-screen
#7
Gilbert G G Donders, Kateryna Ruban, Gert Bellen, Ljubomir Petricevic
Mycoplasmata have been linked to pregnancy complications and neonatal risk. While formerly a limited number of species could be discovered by cultures, molecular biology nowadays discovers both lower quantities and more diverse species, making us realize that mycoplasmata are ubiquitous in the vaginal milieu and do not always pose a danger for pregnant women. As the meaning of mycoplasmata in pregnancy is not clear to many clinicians, we summarized the current knowledge about the meaning of different kinds of mycoplasmata in pregnancy and discuss the potential benefits and disadvantages of treatment...
January 18, 2017: Journal of Perinatal Medicine
https://www.readbyqxmd.com/read/28094842/impact-of-microbial-invasion-of-amniotic-cavity-and-the-type-of-microorganisms-on-short-term-neonatal-outcome-in-women-with-preterm-labor-and-intact-membranes
#8
Teresa Cobo, Irene Vives, Adriano Rodríguez-Trujillo, Clara Murillo, Martina A Ángeles, Jordi Bosch, Andrea Vergara, Eduard Gratacós, Montse Palacio
INTRODUCTION: The objective was to evaluate the impact of microbial invasion of the amniotic cavity (MIAC) and the type of microorganisms on pregnancy and short-term neonatal outcomes in women with preterm labor (PTL). MATERIAL AND METHODS: Prospective observational cohort study including women with PTL from 22.0-36.0 weeks. MIAC was defined based on amniotic fluid aerobic/anaerobic/mycoplasmas cultures and intra-amniotic inflammation (IAI) based on amniotic fluid Interleukin-6 levels...
January 17, 2017: Acta Obstetricia et Gynecologica Scandinavica
https://www.readbyqxmd.com/read/28088841/intracellular-fate-of-ureaplasma-parvum-entrapped-by-host-cellular-autophagy
#9
Fumiko Nishiumi, Michinaga Ogawa, Yukiko Nakura, Yusuke Hamada, Masahiro Nakayama, Jiro Mitobe, Atsushi Hiraide, Norio Sakai, Makoto Takeuchi, Tamotsu Yoshimori, Itaru Yanagihara
Genital mycoplasmas, including Ureaplasma spp., are among the smallest human pathogenic bacteria and are associated with preterm birth. Electron microscopic observation of U. parvum showed that these prokaryotes have a regular, spherical shape with a mean diameter of 146 nm. U. parvum was internalized into HeLa cells by clathrin-mediated endocytosis and survived for at least 14 days around the perinuclear region. Intracellular U. parvum reached endosomes in HeLa cells labeled with EEA1, Rab7, and LAMP-1 within 1 to 3 hr...
January 15, 2017: MicrobiologyOpen
https://www.readbyqxmd.com/read/28070089/development-of-a-one-run-real-time-pcr-detection-system-for-pathogens-associated-with-bovine-respiratory-disease-complex
#10
Mai Kishimoto, Shinobu Tsuchiaka, Sayed Samim Rahpaya, Ayako Hasebe, Keiko Otsu, Satoshi Sugimura, Suguru Kobayashi, Natsumi Komatsu, Makoto Nagai, Tsutomu Omatsu, Yuki Naoi, Kaori Sano, Sachiko Okazaki-Terashima, Mami Oba, Yukie Katayama, Reiichiro Sato, Tetsuo Asai, Tetsuya Mizutani
Bovine respiratory disease complex (BRDC) is frequently found in cattle worldwide. The etiology of BRDC is complicated by infections with multiple pathogens, making identification of the causal pathogen difficult. Here, we developed a detection system by applying TaqMan real-time PCR (Dembo respiratory-PCR) to screen a broad range of microbes associated with BRDC in a single run. We selected 16 bovine respiratory pathogens (bovine viral diarrhea virus, bovine coronavirus, bovine parainfluenza virus 3, bovine respiratory syncytial virus, influenza D virus, bovine rhinitis A virus, bovine rhinitis B virus, bovine herpesvirus 1, bovine adenovirus 3, bovine adenovirus 7, Mannheimia haemolytica, Pasteurella multocida, Histophilus somni, Trueperella pyogenes, Mycoplasma bovis and Ureaplasma diversum) as detection targets and designed novel specific primer-probe sets for nine of them...
January 8, 2017: Journal of Veterinary Medical Science
https://www.readbyqxmd.com/read/28027001/a-case-of-rectal-ureaplasma-infection-and-implications-for-testing-in-young-men-who-have-sex-with-men-the-p18-cohort-study
#11
Collette E Abbott, Richard E Greene, Farzana Kapadia, Perry N Halkitis
Ureaplasma is a significant cause of nongonococcal urethritis. This is a case of rectal Ureaplasma found on culture in a young man who has sex with men not previously reported in the literature. Nucleic acid amplification tests are now standard of care for sexually transmitted infection testing, but they do not test for Ureaplasma and, therefore, may be missing important infections. Ureaplasma could have important implications in urethritis and rectal HIV transmission among men who have sex with men engaging in condomless anal intercourse...
December 27, 2016: LGBT Health
https://www.readbyqxmd.com/read/28012250/the-effect-of-ureaplasma-urealyticum-on-the-level-of-p34h-expression-the-activity-of-hyaluronidase-and-dna-fragmentation-in-human-spermatozoa
#12
Xiao-Ping Ma, Xiao-Qin Gao
BACKGROUND: To study the effect of Ureaplasma urealyticum on the level of P34H expression, the activity of hyaluronidase, and the DNA fragmentation in human spermatozoa. METHOD: Western blot was used to detect the level of P34H expression on spermatozoa.The localization of this protein on human spermatozoa was determined by indirect immunofluorescent and observed by laser scanning confocal microscope. The activity of hyaluronidase was examined by improved fixed-substrate film method...
January 2017: American Journal of Reproductive Immunology: AJRI
https://www.readbyqxmd.com/read/27992658/antimicrobial-activity-of-manuka-honey-against-antibiotic-resistant-strains-of-the-cell-wall-free-bacteria-ureaplasma-parvum-and-ureaplasma-urealyticum
#13
K L Hillitt, R E Jenkins, O B Spiller, M L Beeton
: The susceptibility of the cell wall-free bacterial pathogens Ureaplasma spp. to Manuka honey was examined. The minimum inhibitory concentration (MIC) of Manuka honey for four Ureaplasma urealyticum and four Ureaplasma parvum isolates was determined. Sensitivity to honey was also compared to clinical isolates with resistance to tetracycline, macrolide and fluoroquinolone antibiotics. Finally step-wise resistance training was utilized in an attempt to induce increased tolerance to honey...
December 19, 2016: Letters in Applied Microbiology
https://www.readbyqxmd.com/read/27981387/-non-viral-sexually-transmitted-infections-epidemiology-clinical-manifestations-diagnostics-and-therapy-part%C3%A2-2-chlamydia-and-mycoplasma
#14
REVIEW
P Nenoff, A Manos, I Ehrhard, C Krüger, U Paasch, P Helmbold, W Handrick
Chlamydia trachomatis is the most common pathogen of sexually transmitted bacterial infections worldwide. Every year in Germany approximately 300,000 new infections are to be expected. Chlamydia infections occur nearly exclusively in the postpubertal period. The peak age group is 15-25 years. The infection usually runs an asymptomatic course and the diagnosis is made by nucleic acid amplification techniques (NAAT) often after chlamydial screening or if complications occur. For treatment of chlamydial infections oral doxycycline 100 mg twice daily over 7 days is initially used or alternatively oral azithromycin 1...
December 15, 2016: Der Hautarzt; Zeitschrift Für Dermatologie, Venerologie, und Verwandte Gebiete
https://www.readbyqxmd.com/read/27974410/the-human-ureaplasma-species-as-causative-agents-of-chorioamnionitis
#15
REVIEW
Emma L Sweeney, Samantha J Dando, Suhas G Kallapur, Christine L Knox
The human Ureaplasma species are the most frequently isolated microorganisms from the amniotic fluid and placentae of women who deliver preterm and are also associated with spontaneous abortions or miscarriages, neonatal respiratory diseases, and chorioamnionitis. Despite the fact that these microorganisms have been habitually found within placentae of pregnancies with chorioamnionitis, the role of Ureaplasma species as a causative agent has not been satisfactorily explained. There is also controversy surrounding their role in disease, particularly as not all women infected with Ureaplasma spp...
January 2017: Clinical Microbiology Reviews
https://www.readbyqxmd.com/read/27915439/presence-of-ureaplasma-diversum-in-the-genital-tracts-of-female-dairy-cattle-in-mato-grosso-state-brazil
#16
Jaqueline B Azevedo, Gustavo S Silva, Priscylla S Rocha, Letícia C Pitchenin, Valéria Dutra, Luciano Nakazato, Anderson Castro Soares de Oliveira, Caroline A Pescador
Ureaplasma diversum infection in bovine females may result in various reproductive problems, including granular vulvovaginitis, abortion, weak calves, salpingitis, and spontaneous abortion. The presence of U. diversum in a dairy bovine population from midwestern Brazil has not been established. The aim of this study was to determine whether U. diversum was present in dairy cattle from midwestern Brazil using polymerase chain reaction (PCR). Vulvovaginal mucus was analyzed from 203 cows located in six municipalities in the north region of Mato Grosso State, Brazil...
February 2017: Tropical Animal Health and Production
https://www.readbyqxmd.com/read/27911430/-clinical-presentations-of-herpes-zoster-ophthalmicus-diagnosis-and-therapy
#17
G M Chernakova, E A Kleshcheva, T B Semenova
: Approximately a quarter of the world's population at some point in life is at risk of developing shingles (Herpes Zoster). In 10-20% of cases the first branch of the trigeminal nerve gets involved (Herpes Zoster Ophthalmicus, HZO). Ophthalmic complications of HZO are able to cause a significant reduction in visual function. AIM: To study and summarize clinical features of HZO (including the rate of complications and their nature) and to determine the relationship between clinical and laboratory data from these patients...
2016: Vestnik Oftalmologii
https://www.readbyqxmd.com/read/27884651/analysis-of-mutations-in-dna-gyrase-and-topoisomerase-iv-of-ureaplasma-urealyticum-and-ureaplasma-parvum-serovars-resistant-to-fluoroquinolones
#18
Giorgio Piccinelli, Franco Gargiulo, Valeria Biscaro, Francesca Caccuri, Arnaldo Caruso, Maria Antonia De Francesco
This study aims to determine the prevalence of fluoroquinolone resistance of Ureaplasma biovars and serovars isolated from urogenital clinical samples and determine the underlying molecular mechanism for quinolone resistance for all resistant isolates. Of 105 samples confirmed as positive for U. urealyticum/U. parvum, 85 were resistant to quinolones by the Mycoplasma-IST2 kit. However, only 43 out of 85 quinolone resistant isolates had amino acid substitutions in GyrA, GyrB, ParC and ParE proteins underlining that this assay have mis-identified as fluoroquinolone resistant 42 isolates...
January 2017: Infection, Genetics and Evolution
https://www.readbyqxmd.com/read/27870156/combinations-and-loads-of-bacteria-affect-the-cytokine-production-by-fetal-membranes-an-in-vitro-study
#19
Nathalia Mayumi Noda-Nicolau, Jossimara Polettini, Morgan R Peltier, Márcia Guimarães da Silva, Ramkumar Menon
PROBLEM: The polybacterial invasion and inflammation of the amniotic cavity is a common scenario in PTB, and then, we analyzed the cytokine production by human fetal membranes to better understand the host response to polybacterial infections. METHOD OF STUDY: Fetal membranes were treated by heat-inactivated genital mycoplasmas and Gardnerella vaginalis at 10(3) or 10(6) colony/color-forming units/mL alone or in combination. Cytokines/receptors were measured in the medium by immunoassays...
December 2016: American Journal of Reproductive Immunology: AJRI
https://www.readbyqxmd.com/read/27862576/foetal-ureaplasma-parvum-bacteraemia-as-a-function-of-gestation-dependent-complement-insufficiency-evidence-from-a-sheep-model-of-pregnancy
#20
Matthew W Kemp, Shatha Ahmed, Michael L Beeton, Matthew S Payne, Masatoshi Saito, Yuichiro Miura, Haruo Usuda, Suhas G Kallapur, Boris W Kramer, Sarah J Stock, Alan H Jobe, John P Newnham, Owen B Spiller
PROBLEM: Complement is a central defence against sepsis, and increasing complement insufficiency in neonates of greater prematurity may predispose to increased sepsis. Ureaplasma spp. are the most frequently cultured bacteria from preterm blood samples. METHOD OF STUDY: A sheep model of intrauterine Ureaplasma parvum infection was used to examine in vivo Ureaplasma bacteraemia at early and late gestational ages. Complement function and Ureaplasma killing assays were used to determine the correlation between complement potency and bactericidal activity of sera ex vivo...
January 2017: American Journal of Reproductive Immunology: AJRI
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