Read by QxMD icon Read


Judith Kikhney, Dinah von Schöning, Ina Steding, Julia Schulze, Annett Petrich, Andreas Hiergeist, Udo Reischl, Annette Moter, Anke Thomas
OBJECTIVES: The aim of this study was to detect microorganisms in foetal membranes and placental tissue in preterm chorioamnionitis by combining FISH with broad range PCR. The combination of the two molecular techniques enables identification and localization of the microorganisms within the tissue, confirming their clinical relevance. METHODS: In a prospective cohort study, we compared 31 patients with preterm premature rupture of membranes or preterm labour and preterm delivery by caesarean section with a control group of 26 participants with elective caesarean section at term...
October 15, 2016: Clinical Microbiology and Infection
Matthew S Payne, Demelza J Ireland, Rory Watts, Elizabeth A Nathan, Lucy L Furfaro, Matthew W Kemp, Jeffrey A Keelan, John P Newnham
BACKGROUND: Detection of Ureaplasma, Mycoplasma and Candida spp. in the vagina during pregnancy has previously been associated with preterm birth (PTB). However, the prevalence of these microorganisms and the associated obstetric risks (likely to be population-specific) have not been determined in Australian women; furthermore, in the case of Ureaplasma spp., very few studies have attempted characterisation at the species level and none have examined genotype/serovar status to further refine risk assessment...
October 18, 2016: BMC Pregnancy and Childbirth
Qing-Zhen Xie, Wang-Ming Xu, Qian-Rong Qi, Zeng-Li Luo, Lan Dong
Genital tract infections with ureaplasma urealyticum (UU) and chlamydia trachomatis (CT) are the most frequent sexually-transmitted disease worldwide. UU and CT infections are considered to be the leading cause for infertility and adverse pregnancy outcomes. However, little is known about the specific effect of cervical UU and CT infections on the etiology of female infertility, as well as the pregnancy outcomes of the patients undergoing in vitro fertilization/intracytoplasmic sperm injection-embryo transfer (IVF/ICSI-ET)...
October 2016: Journal of Huazhong University of Science and Technology. Medical Sciences
Ping Zhou, Rui Chen, Cai-Yan Zou, Min Zhang, Zhao-Hong Chen, Li-Ya Ma
OBJECTIVE: To study the postnatal changes in lymphocyte subsets in early preterm infants and the effect of perinatal factors on lymphocyte subsets. METHODS: A total of 61 early preterm infants were enrolled. Flow cytometry was used to measure the absolute counts of lymphocytes and lymphocyte subsets at 1, 7, 14, and 28 days after birth, as well as at 6 months after birth for 17 of these early preterm infants. The effects of perinatal factors, such as antepartum use of hormone, intrauterine infection, gestational age at birth, and Ureaplasma urealyticum (UU) colonization, on lymphocyte subsets were analyzed...
October 2016: Zhongguo Dang Dai Er Ke za Zhi, Chinese Journal of Contemporary Pediatrics
Pingnan Shan, Zhiyong Lu, Lihong Ye, Yaqin Fang, Suhong Tan, Guohong Xuan, Jincheng Ru, Liming Mao
BACKGROUND Prostatitis is a common and refractory urological disease with complicated etiology. Ureaplasma urealyticum (UU) has a close relationship with human urinary tract infection that can induce nonbacterial prostatitis. Tripterygium wilfordii polyglycoside (TWP) is a non-steroidal immune inhibitor that causes significant immune suppression and anti-inflammatory effects. Its role in prostatitis caused by UU has not yet been established. The aim of this study was to investigate the effect of TWP on UU-infected prostatitis in a rat model...
October 15, 2016: Medical Science Monitor: International Medical Journal of Experimental and Clinical Research
Nicholay A Shcherbina, Liudmyla A Vygovskaya
INTRODUCTION: the relevance of intrauterine infections is determined by significant peri- and postnatal loss as well as health impairment, which often results in disability and reduced quality of life. Ultrasonography is employed in order to provide a reliable assessment of the functional state of the fetoplacental system secondary to intrauterine fetal infection in the course of pregnancy. Ultrasound imaging is essential in diagnosis of various preclinical complications of pregnancy and detection of abnormalities in the developing fetus...
2016: Wiadomości Lekarskie: Organ Polskiego Towarzystwa Lekarskiego
Ken B Waites, Donna M Crabb, Yang Liu, Lynn B Duffy
In vitro activities of omadacycline, a new aminomethylcycline, were determined for Mycoplasma and Ureaplasma spp. in comparison to azithromycin, clindamycin, moxifloxacin, tetracycline, and doxycycline. All omadacycline MICs were < 2 μg/ml. MIC90s were 0.063 μg/ml for Mycoplasma hominis, 0.25 μg/ml for Mycoplasma pneumoniae, and 2 μg/ml for Ureaplasma spp. Omadacycline had the lowest MIC90 among all drugs tested against M. hominis Omadacycline activity was not affected by macrolide, tetracycline, or fluoroquinolone resistance...
September 26, 2016: Antimicrobial Agents and Chemotherapy
Charitharth V Lal, Xin Xu, Patricia Jackson, Thomas P Atkinson, Ona M Faye-Petersen, Jegen Kandasamy, Ken Waites, Joseph R Biggio, Amit Gaggar, Namasivayam Ambalavanan
BACKGROUND: Premature rupture of membranes and preterm delivery are associated with Ureaplasma infection. We hypothesized that Ureaplasma induced extracellular collagen fragmentation results in production of the tripeptide PGP (proline-glycine-proline), a neutrophil chemoattractant. PGP release from collagen requires matrix metalloproteases (MMP-8/MMP-9) along with a serine protease, prolyl endopeptidase (PE). METHODS: Ureaplasma culture negative amniotic fluid (indicated preterm birth, n=8; spontaneous preterm birth, n=8) and Ureaplasma positive amniotic fluid (spontaneous preterm birth, n=8) were analyzed by electro-spray ionization-liquid chromatography tandem mass spectrometry for PGP, and for MMP-9 by zymography...
September 15, 2016: Pediatric Research
Filipa Godoy-Vitorino, Arnold Rodriguez-Hilario, Ana Luísa Alves, Filipa Gonçalves, Beatriz Cabrera-Colon, Cristina Sousa Mesquita, Pedro Soares-Castro, Marisa Ferreira, Ana Marçalo, José Vingada, Catarina Eira, Pedro Miguel Santos
Infectious diseases with epizootic consequences have not been fully studied in marine mammals. Presently, the unprecedented depth of sequencing, made available by high-throughput approaches, allows detailed comparisons of the microbiome in health and disease. This is the first report of the striped dolphin microbiome in different body sites. Samples from one striped female edematous dolphin were acquired from a variety of body niches, including the blowhole, oral cavity, oral mucosa, tongue, stomach, intestines and genital mucosa...
September 9, 2016: Research in Microbiology
Lucas M Marques, Izadora S Rezende, Maysa S Barbosa, Ana M S Guimarães, Hellen B Martins, Guilherme B Campos, Naíla C do Nascimento, Andrea P Dos Santos, Aline T Amorim, Verena M Santos, Sávio T Farias, Fernanda  C Barrence, Lauro M de Souza, Melissa Buzinhani, Victor E Arana-Chavez, Maria E Zenteno, Gustavo P Amarante-Mendes, Joanne B Messick, Jorge Timenetsky
Whole genome sequencing and analyses of Ureaplasma diversum ATCC 49782 was undertaken as a step towards understanding U. diversum biology and pathogenicity. The complete genome showed 973,501 bp in a single circular chromosome, with 28.2% of G+C content. A total of 782 coding DNA sequences (CDSs), and 6 rRNA and 32 tRNA genes were predicted and annotated. The metabolic pathways are identical to other human ureaplasmas, including the production of ATP via hydrolysis of the urea. Genes related to pathogenicity, such as urease, phospholipase, hemolysin, and a Mycoplasma Ig binding protein (MIB)-Mycoplasma Ig protease (MIP) system were identified...
2016: PloS One
Paranthaman Senthamaraikannan, Pietro Presicce, Cesar M Rueda, Gunlawadee Maneenil, Augusto F Schmidt, Lisa A Miller, Ken B Waites, Alan H Jobe, Suhas G Kallapur, Claire A Chougnet
BACKGROUND:  Although Ureaplasma species are the most common organisms associated with prematurity, their effects on the maternal and fetal immune system remain poorly characterized. METHODS:  Rhesus macaque dams at ∼80% gestation were injected intra-amniotically with 10(7) CFU Ureaplasma parvum (UP) or saline (control). Fetuses were delivered surgically 3 or 7 days later. We performed comprehensive assessments of inflammation and immune effects in multiple fetal and maternal tissues...
September 6, 2016: Journal of Infectious Diseases
Cecilia Stalberg, Nathalia Noda, Jossimara Polettini, Bo Jacobsson, Ramkumar Menon
OBJECTIVE: Elafin is a low molecular weight protein with antileukoproteinase, anti-inflammatory, antibacterial and immunomodulating properties. The profile of Elafin in fetal membranes is not well characterized. This study determined the changes in Elafin expression and concentration in human fetal membrane from patients with preterm prelabor rupture of membranes (PPROM) and in vitro in response to intra-amniotic polymicrobial pathogens. METHOD: Elafin messenger RNA (mRNA) expressions were studied in fetal membranes from PPROM, normal term as well as in normal term not in labor membranes in an organ explant system treated (24 h) with lipopolysaccharide (LPS), using quantitative reverse transcription-polymerase chain reaction (RT-PCR)...
August 31, 2016: Journal of Perinatal Medicine
Y Lu, C Z Rong, J Y Zhao, X J Lao, L Xie, S Li, X Qin
The shipment and storage conditions of clinical samples pose a major challenge to the detection accuracy of Chlamydia trachomatis (CT), Neisseria gonorrhoeae (NG), and Ureaplasma urealyticum (UU) when using quantitative real-time polymerase chain reaction (qRT-PCR). The aim of the present study was to explore the influence of storage time at 4°C on the DNA of these pathogens and its effect on their detection by qRT-PCR. CT, NG, and UU positive genital swabs from 70 patients were collected, and DNA of all samples were extracted and divided into eight aliquots...
August 25, 2016: Brazilian Journal of Medical and Biological Research, Revista Brasileira de Pesquisas Médicas e Biológicas
Harald Moi, Nils Reinton, Ivana Randjelovic, Elina J Reponen, Line Syvertsen, Amir Moghaddam
A non-syndromic approach to treatment of people with non-gonococcal urethritis (NGU) requires identification of pathogens and understanding of the role of those pathogens in causing disease. The most commonly detected and isolated micro-organisms in the male urethral tract are bacteria belonging to the family of Mycoplasmataceae, in particular Ureaplasma urealyticum and Ureaplasma parvum To better understand the role of these Ureaplasma species in NGU, we have performed a prospective analysis of male patients voluntarily attending a drop in STI clinic in Oslo...
August 24, 2016: International Journal of STD & AIDS
Ciara Cox, Alison P Watt, James P McKenna, Peter V Coyle
The numbers of rectal sexually transmitted infections are on the rise especially among men who have sex with men. Males from men who have sex with men population are encouraged to send a rectal swab to the laboratory for sexually transmitted infection screening at their visit to the Genitourinary Medicine Clinic. In healthy asymptomatic males, the range of pathogens tested is limited therefore other pathogens may be left untreated allowing infections to persist among sexual partners. Molecular techniques have revolutionarised sexually transmitted infection testing enabling the detection of previously difficult-to-culture pathogens in extra-genital sites and have increased the evidence base for their clinical significance...
August 19, 2016: International Journal of STD & AIDS
Xiaohui Wang, Melissa J Karau, Kerryl E Greenwood-Quaintance, Darci R Block, Jayawant N Mandrekar, Scott A Cunningham, Robin Patel
Hyperammonemia syndrome is an often fatal complication of lung transplantation which has been recently associated with Ureaplasma infection. It has not been definitely established that Ureaplasma species can cause hyperammonemia. We established a novel immunocompromised murine model of Ureaplasma urealyticum infection and used it to confirm that U. urealyticum can cause hyperammonemia. Male C3H mice were pharmacologically immunosuppressed with mycophenolate mofetil, tacrolimus and oral prednisone for seven days, and then challenged intratracheally (IT) and/or intraperitoneally (IP) with 107 CFU U...
2016: PloS One
Yingying Kong, Yingli Qiao, Jingjuan Song, Zhi Ruan, Chunrong Fei, Jun Huang, Tiejun Song, Hong Jin, Honghui Ding, Xinyou Xie, Jun Zhang
The aim of this study was to estimate the prevalence and antimicrobial resistance rate of Mycoplasma hominis among male and female populations. A total of 67921 individuals were examined. All samples were isolated from patients at an outpatient clinic from January 2005 to December 2014. Species identification and antibiotic susceptibility testing were performed using Mycoplasma IST2. In this study, 523 (0.8%) and 4625 (6.8%) cultures, respectively, were positive for single M. hominis identification and simultaneous identification of both M...
September 2016: Journal of Global Antimicrobial Resistance
X-Y Zeng, N Xin, X-N Tong, J-Y Wang, Z-W Liu
This study analyzed the prevalence and antibiotic susceptibility of urogenital Ureaplasma urealyticum and Mycoplasma hominis isolated in Xi'an, China. A total of 2161 individuals from 2011 to 2015 were included, and antibiotic susceptibility tests were performed by using the Mycoplasma IST kit. Of the individuals studied, 1018 (47.11 %) were identified to be positive for urogenital mycoplasmas. The single U. urealyticum, single M. hominis, and dual U. urealyticum and M. hominis infections accounted for 772 (75...
August 16, 2016: European Journal of Clinical Microbiology & Infectious Diseases
Gholamreza Irajian, Mehri Sharifi, Shiva Mirkalantari, Reza Mirnejad, Mohammad Reza Jalali Nadoushan, Nafiseh Ghorbanpour
BACKGROUND: In most cases, prostatitis can be caused by a bacterial agent such as Ureaplasma urealyticum. Considering to the cumbersome of the culture method for the detection of Ureaplasma species in clinical samples such as prostate; PCR method that is faster and more appropriate than the cultivation methods, can be utilized for the detection of U. urealyticum and U. parvum. PCR-RFLP method can differentiate both biovars and assist in studies of the clinical diagnosis, epidemiology and pathology of this species in human...
2016: Iranian Journal of Pathology
Xiaofei Zhu, Min Li, Huiling Cao, Xuewen Yang, Chunbing Zhang
The aim of the present study was to investigate the association between Mycoplasma infection and infertility in male outpatients among a Chinese population. Epidemiological data, including prevalence, age distribution and antibiotic resistance profile of patients with an Ureaplasma urealyticum or Mycoplasma hominis infection were collected between 2009 and 2012. Among the 7,374 individuals analyzed, 3,225 patients (43.7%) were determined to be positive for infection with U. urealyticum, M. hominis or for both Mycoplasmas...
August 2016: Experimental and Therapeutic Medicine
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"