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Ureaplasma

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https://www.readbyqxmd.com/read/29236848/histopatology-of-the-reproductive-tract-of-nellore-pubertal-heifers-with-genital-ureaplasmosis
#1
Regiani Pôrto, Benedito Oliveira Filho, Henrique Ferraz, Luciano Caixeta, Marco Antonio Viu, Maria Lúcia Gambarini
In order to study and characterize the lesions in the reproductive tract of Nellore heifers naturally infected with Ureaplasma diversum and presenting granular vulvovaginitis syndrome (GVS), fragments of uterine tube, uterus, cervix, vagina and vulva of 20 animals were evaluated. The macroscopic lesions of the vulvovaginal mucosa were classified in scores of "1" mild, until "4", severe inflammation and pustular or necrotic lesions. The histopathological evaluation was performed using scores of "1" to "4", according to the inflammatory alterations...
December 7, 2017: Anais da Academia Brasileira de Ciências
https://www.readbyqxmd.com/read/29234642/ureaplasma-species-differentially-modulate-pro-and-anti-inflammatory-cytokine-responses-in-newborn-and-adult-human-monocytes-pushing-the-state-toward-pro-inflammation
#2
Kirsten Glaser, Christine Silwedel, Markus Fehrholz, Ana M Waaga-Gasser, Birgit Henrich, Heike Claus, Christian P Speer
Background:Ureaplasma species have been associated with chorioamnionitis and preterm birth and have been implicated in the pathogenesis of neonatal short and long-term morbidity. However, being mostly commensal bacteria, controversy remains on the pro-inflammatory capacity of Ureaplasma. Discussions are ongoing on the incidence and impact of prenatal, perinatal, and postnatal infection. The present study addressed the impact of Ureaplasma isolates on monocyte-driven inflammation. Methods: Cord blood monocytes of term neonates and adult monocytes, either native or LPS-primed, were cultured with Ureaplasma urealyticum (U...
2017: Frontiers in Cellular and Infection Microbiology
https://www.readbyqxmd.com/read/29232399/maternal-white-blood-cell-count-cannot-identify-the-presence-of-microbial-invasion-of-the-amniotic-cavity-or-intra-amniotic-inflammation-in-women-with-preterm-prelabor-rupture-of-membranes
#3
Ivana Musilova, Lenka Pliskova, Romana Gerychova, Petr Janku, Ondrej Simetka, Petr Matlak, Bo Jacobsson, Marian Kacerovsky
OBJECTIVE: The main aim of this study was to determine the relationship between the maternal white blood cell (WBC) count at the time of hospital admission in pregnancies complicated by preterm prelabor rupture of membranes (PPROM) and the presence of microbial invasion of the amniotic cavity (MIAC) and/or intra-amniotic inflammation (IAI). The second aim was to test WBC diagnostic indices with respect to the presence of MIAC and/or IAI. METHODS: Four hundred and seventy-nine women with singleton pregnancies complicated by PPROM, between February 2012 and June 2017, were included in this study...
2017: PloS One
https://www.readbyqxmd.com/read/29225146/microbial-diversity-within-the-airway-microbiome-in-chronic-pediatric-lung-diseases
#4
Andrea Hahn, Stephanie Warnken, Marcos Pérez-Losada, Robert J Freishtat, Keith A Crandall
The study of the airway microbiome in children is an area of emerging research, especially in relation to the role microbial diversity may play in acute and chronic inflammation. Three such pediatric airway diseases include cystic fibrosis, asthma, and chronic lung disease of prematurity. In cystic fibrosis, the presence of Pseudomonas spp. is associated with decreased microbial diversity. Decreasing microbial diversity is also associated with poor lung function. In asthma, early viral infections appear to drive changes in bacterial diversity which may be associated with asthma risk...
December 7, 2017: Infection, Genetics and Evolution
https://www.readbyqxmd.com/read/29191491/a-nested-pcr-strategy-for-molecular-diagnosis-of-mollicutes-in-uncultured-biological-samples-from-cows-with-vulvovaginitis
#5
Daniele Cristina Voltarelli, Brígida Kussumoto de Alcântara, Michele Lunardi, Alice Fernandes Alfieri, Raquel de Arruda Leme, Amauri Alcindo Alfieri
Bacteria classified in Mycoplasma (M. bovis and M. bovigenitalium) and Ureaplasma (U. diversum) genera are associated with granular vulvovaginitis that affect heifers and cows at reproductive age. The traditional means for detection and speciation of mollicutes from clinical samples have been culture and serology. However, challenges experienced with these laboratory methods have hampered assessment of their impact in pathogenesis and epidemiology in cattle worldwide. The aim of this study was to develop a PCR strategy to detect and primarily discriminate between the main species of mollicutes associated with reproductive disorders of cattle in uncultured clinical samples...
November 24, 2017: Animal Reproduction Science
https://www.readbyqxmd.com/read/29182058/prevalence-and-antimicrobial-susceptibility-of-ureaplasma-species-and-mycoplasma-hominis-in-greek-female-outpatients-2012-2016
#6
Sofia Maraki, Viktoria Eirini Mavromanolaki, Eleni Nioti, Dimitra Stafylaki, George Minadakis
Mycoplasma hominis and Ureaplasma species are opportunistic pathogens associated with urogenital infections, complications during pregnancy and postpartum infections. Appropriate empirical antimicrobial treatment is necessary to achieve an optimal therapeutic outcome. This study evaluated the prevalence and the antimicrobial susceptibility of Mycoplasma hominis and Ureaplasma spp. isolated from 1,008 endocervical samples of outpatients in Crete, Greece, during a five-year period (2012-2016), using the commercially available Mycoview kit (Zeakon diagnostics, France)...
November 28, 2017: Journal of Chemotherapy
https://www.readbyqxmd.com/read/29157044/prenatal-complications-are-associated-with-the-postnatal-airway-host-response-and-microbiota-in-intubated-preterm-infants
#7
Brandie D Wagner, Marci K Sontag, J Kirk Harris, Joshua I Miller, Lindsey Morrow, Charles E Robertson, Mark J Stephens, Brenda B Poindexter, Steven H Abman, Peter M Mourani
PURPOSE: To prospectively examine the relationship between prenatal events, postnatal airway host response and microbiota, and clinical outcomes. MATERIALS AND METHODS: Tracheal aspirates collected at 7 days of age from 71 mechanically ventilated infants (median gestational age (GA), 25 weeks [range 23-28]) were simultaneously processed for a 12-plex protein assay and bacterial identification by 16S rRNA sequencing. Phenotypes were determined by unsupervised clustering of the protein analytes...
November 20, 2017: Journal of Maternal-fetal & Neonatal Medicine
https://www.readbyqxmd.com/read/29155238/evaluation-of-two-real-time-pcr-assays-for-the-detection-of-bacterial-dna-in-amniotic-fluid
#8
Patricia Girón de Velasco-Sada, Iker Falces-Romero, Inmaculada Quiles-Melero, Adela García-Perea, Jesús Mingorance
PURPOSE: The aim of this study was to evaluate two non-commercial Real-Time PCR assays for the detection of microorganisms in amniotic fluid followed by identification by pyrosequencing. METHODS: We collected 126 amniotic fluids from 2010 to 2015 for the evaluation of two Real-Time PCR assays for detection of bacterial DNA in amniotic fluid (16S Universal PCR and Ureaplasma spp. specific PCR). The method was developed in the Department of Microbiology of the University Hospital La Paz...
November 16, 2017: Journal of Microbiological Methods
https://www.readbyqxmd.com/read/29154392/rapid-and-simple-detection-of-ureaplasma-species-from-vaginal-swab-samples-using-a-loop-mediated-isothermal-amplification-method
#9
Kazumasa Fuwa, Mitsuko Seki, Yoshiyasu Hirata, Itaru Yanagihara, Yukiko Nakura, Chika Takano, Kazumichi Kuroda, Satoshi Hayakawa
PROBLEM: Ureaplasma species occasionally cause chorioamnionitis and premature labor. We developed a novel assay employing a loop-mediated isothermal amplification (LAMP) method to detect Ureaplasma parvum and Ureaplasma urealyticum. METHOD OF STUDY: Loop-mediated isothermal amplification primers were designed to amplify Ureaplasma-specific ureaseB genes. Four U. parvum strains, 5 U. urealyticum strains and 14 reference bacterial species were evaluated. Forty-six vaginal swab samples were analyzed by LAMP, culture, and PCR...
November 20, 2017: American Journal of Reproductive Immunology: AJRI
https://www.readbyqxmd.com/read/29154180/a-twelve-year-retrospective-analysis-of-prevalence-and-antimicrobial-susceptibility-patterns-of-ureaplasma-spp-and-mycoplasma-hominis-in-the-province-of-lower-silesia-in-poland
#10
Urszula Kasprzykowska, Beata Sobieszczańska, Anna Duda-Madej, Anna Secewicz, Joanna Nowicka, Grażyna Gościniak
OBJECTIVE: Genital mycoplasmas are opportunistic pathogens that have been associated with urogenital infections in humans. Only a few groups of antimicrobials are available for treatment of urogenital tract infections caused by genital mycoplasmas. However, emerging resistance of mycoplasmas to antimicrobial agents has been reported worldwide. The aim of the study was a retrospective analysis of the prevalence and antimicrobial susceptibility patterns of M. hominis and Ureaplasma spp...
November 14, 2017: European Journal of Obstetrics, Gynecology, and Reproductive Biology
https://www.readbyqxmd.com/read/29153035/an-unusual-presentation-of-pseudocowpox-associated-with-an-outbreak-of-pustular-ulcerative-vulvovaginitis-in-a-swedish-dairy-herd
#11
Gunilla Blomqvist, Karin Ullman, Thomas Segall, Elenor Hauzenberger, Lena Renström, Karin Persson-Waller, Mikael Leijon, Jean-Francois Valarcher
Species Pseudocowpox virus (PCPV; family Poxviridae) is known to cause pustular cutaneous disease in cattle. We describe an outbreak of pseudocowpox with an unusual clinical picture in a free-stall dairy herd of ~80 cows. Approximately 90% of the cows had vesicles, erosions, papules, and scabs on the vulva and vaginal mucosa. Histologic analysis of biopsy tissues indicated a primary, although not specified, viral infection. Transmission electron microscopy revealed parapoxvirus particles in both tissue and vesicular materials...
November 1, 2017: Journal of Veterinary Diagnostic Investigation
https://www.readbyqxmd.com/read/29145645/waddlia-chondrophila-a-chlamydia-related-bacterium-has-a-negative-impact-on-human-spermatozoa
#12
D Baud, N Vulliemoz, A Ammerdorffer, J Gyger, G Greub, V Castella, M Stojanov
STUDY QUESTION: What is the impact of Waddlia chondrophila, an emerging Chlamydia-related bacterium associated with miscarriage, on human spermatozoa? SUMMARY ANSWER: W. chondrophila had a negative impact on human spermatozoa (decrease in viability and mitochondrial membrane potential) and was not entirely removed from infected samples by density gradient centrifugation. WHAT IS KNOWN ALREADY: Bacterial infection or colonization might have a deleterious effect on male fertility...
November 14, 2017: Human Reproduction
https://www.readbyqxmd.com/read/29124540/oligonucleotide-microchip-for-the-identification-of-infectious-agents-of-reproductive-system-with-simultaneous-analysis-of-determinants-of-resistance-to-antimicrobial-substances
#13
A T Leinsoo, B L Shaskol'skii, E I Dement'eva, D A Gryadunov, A A Kubanov, A V Chestkov, O A Obraztsova, M V Shpilevaya, D G Deryabin
We developed a multiplexed DNA microarray-based assay allowing identification of 12 causative agents of reproductive tract infections with the simultaneous detection of 47 genetic determinants of resistance to antimicrobial substances. The microarray was tested on 93 isolates of Neisseria gonorrhoeae, 32 isolates of Treponema pallidum and 29 samples of Ureaplasma spp./Mycoplasma spp. The N. gonorrhoeae isolates had multiple mutations in the penA, ponA, rpsJ, gyrA, parC, and mtrR genes; their prognostic value significantly increased when combinations of mutations were detected...
November 10, 2017: Bulletin of Experimental Biology and Medicine
https://www.readbyqxmd.com/read/29052561/a-bayesian-stepwise-discriminant-model-for-predicting-risk-factors-of-preterm-premature-rupture-of-membranes-a-case-control-study
#14
Li-Xia Zhang, Yang Sun, Hai Zhao, Na Zhu, Xing-De Sun, Xing Jin, Ai-Min Zou, Yang Mi, Ji-Ru Xu
BACKGROUND: Preterm premature rupture of membrane (PPROM) can lead to serious consequences such as intrauterine infection, prolapse of the umbilical cord, and neonatal respiratory distress syndrome. Genital infection is a very important risk which closely related with PPROM. The preliminary study only made qualitative research on genital infection, but there was no deep and clear judgment about the effects of pathogenic bacteria. This study was to analyze the association of infections with PPROM in pregnant women in Shaanxi, China, and to establish Bayesian stepwise discriminant analysis to predict the incidence of PPROM...
October 20, 2017: Chinese Medical Journal
https://www.readbyqxmd.com/read/28984242/biochanin-a-partially-restores-the-activity-of-ofloxacin-and-ciprofloxacin-against-topoisomerase-iv-mutation-associated-fluoroquinolone-resistant-ureaplasma-species
#15
Hong Jin, Chao Qi, Yanping Zou, Yingying Kong, Zhi Ruan, Honghui Ding, Xinyou Xie, Jun Zhang
PURPOSE: This study aims to investigate the synergistic antimicrobial activity of four phytoalexins in combination with fluoroquinolones against Ureaplasma spp., a genus of cell wall-free bacteria that are intrinsically resistant to many available antibiotics, making treatment inherently difficult. METHODOLOGY: A total of 22 958 urogenital tract specimens were assessed for Ureaplasma spp. identification and antimicrobial susceptibility. From these, 31 epidemiologically unrelated strains were randomly selected for antimicrobial susceptibility testing to determine the minimum inhibitory concentration (MIC) of four fluoroquinolones and the corresponding quinolone resistance-determining regions (QRDRs)...
October 6, 2017: Journal of Medical Microbiology
https://www.readbyqxmd.com/read/28980570/-mixed-infections-and-inflammatory-ophthalmic-diseases-clinical-and-laboratory-observations
#16
G M Chernakova, D Yu Maychuk, E A Klescheva, Yu B Slonimskiy, T B Semenova
In recent years, all medical specialists, including ophthalmologists, have been facing the problem of mixed infections. Recurrent inflammation in the anterior and posterior eye segments is often a result of infection by more than one variety of pathogens. MATERIAL AND METHODS: Over the period 2013-2016, 34 patients (14 men and 20 women) with different inflammatory processes in the eye who appeared DNA-positive for mycoplasmas (Mycoplasma hominis, Ureaplasma urealyticum) and/or chlamydiae (Chlamydia trachomatis) (PCR testing of tear fluid and/or urine) were followed up...
2017: Vestnik Oftalmologii
https://www.readbyqxmd.com/read/28964561/-identification-of-species-of-mycoplasma-and-ureaplasma-diversum-from-argentinian-dairy-herds
#17
Camila Sosa, Liliana Tirante, Javier Chaves, Martín Pol, Arnaldo Ambrogi, José Angel Giraudo, Pablo Tamiozzo
Several species of Mycoplasma and Ureaplasma diversum can cause diseases in dairy cattle, which can be associated or not with clinical manifestations. In our country, the presence of Mycoplasma bovis, Mycoplasma californicum and Mycoplasma canadense has been detected, being the only mycoplasma species identified so far. The objective of this study was to identify other species of the Mycoplasmataceae family. Thirty-five Mycoplasma spp.-like isolates obtained from different samples from cattle, with or without clinical symptoms, from eight herds located in the provinces of Santa Fe, Cordoba, Buenos Aires and San Luis were utilized in the present study...
September 27, 2017: Revista Argentina de Microbiología
https://www.readbyqxmd.com/read/28960028/prevalence-of-and-risk-factors-for-sexually-transmitted-infections-among-korean-adolescents-under-probation
#18
Jin Ju Park, Yu Bin Seo, Sookyung Jeong, Jacob Lee
There is limited research on sexually transmitted infections (STIs) among adolescents in Korea. The objective of this study was to explore the prevalence of and risk factors for STIs among Korean adolescents under probation. A cross-sectional analysis was conducted in one juvenile-delinquent center and five probation offices in Korea to determine the prevalence of STIs caused by the following pathogens: Chlamydia trachomatis, Neisseria gonorrhoeae, Trichomonas vaginalis, herpes simplex virus (HSV), human immunodeficiency virus (HIV), Treponema pallidum, Mycoplasma hominis, Mycoplasma genitalium, Ureaplasma urealyticum, and Ureaplasma parvum...
November 2017: Journal of Korean Medical Science
https://www.readbyqxmd.com/read/28957335/fetal-skin-as-a-pro-inflammatory-organ-evidence-from-a-primate-model-of-chorioamnionitis
#19
Suppawat Boonkasidecha, Paranthaman Senthamarai Kannan, Suhas G Kallapur, Alan H Jobe, Matthew W Kemp
BACKGROUND: Intrauterine infection is a primary cause of preterm birth and fetal injury. The pro-inflammatory role of the fetal skin in the setting of intrauterine infection remains poorly characterized. Whether or not inflammation of the fetal skin occurs in primates remains unstudied. Accordingly, we hypothesized that: i) the fetal primate skin would mount a pro-inflammatory response to preterm birth associated pro-inflammatory agents (lipopolysaccharides from Escherichia coli, live Ureaplasma parvum, interleukin-1β) and; ii) that inhibiting interleukin-1 signaling would decrease the skin inflammatory response...
2017: PloS One
https://www.readbyqxmd.com/read/28951103/the-value-of-molecular-techniques-to-diagnose-ureaplasma-urealyticum-and-nocardia-farcinica-pleuropneumonia-in-a-patient-with-diffuse-large-b-cell-lymphoma
#20
Etienne Canouï, Kim Blanc, Julien Loubinoux, Sandrine Valade, Cécile Hamard, Aurélie Lefebvre, Sandy Amorim, Cécile Bébéar, Veronica Rodriguez-Nava, David Lebeaux, Odile Launay, Marco Alifano, Antoine Rabbat, Solen Kernéis
An unusual case of pleural empyema related to Nocardia farcinica and Ureaplasma urealyticum, occurring after autologous haematopoietic stem cell transplantation in a 30-year-old patient with lymphoma, is reported. This case illustrates the role of repeated and comprehensive microbiological investigations and the contribution of molecular techniques in reaching the aetiological diagnosis.
September 22, 2017: International Journal of Infectious Diseases: IJID
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