Yasuto Takeuchi, Masayuki Inubushi, Yong-Nan Jin, Chika Murai, Atsushi B Tsuji, Hironobu Hata, Yoshimasa Kitagawa, Tsuneo Saga
OBJECTIVE: HIF-1/HRE pathway is a promising target for the imaging and the treatment of intractable malignancy (HIF-1; hypoxia-inducible factor 1, HRE; hypoxia-responsive element). The purposes of our study are: (1) to assess the gene activation levels resulting from various numbers of HREs under various hypoxic conditions, (2) to evaluate the bidirectional activity of multiple HREs, and (3) to confirm whether multiple HREs can induce gene expression in vivo. METHODS: Human colon carcinoma HCT116 cells were transiently transfected by the constructs containing a firefly luciferase reporter gene and various numbers (2, 4, 6, 8, 10, and 12) of HREs (nHRE+, nHRE-)...
December 2014: Annals of Nuclear Medicine