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https://www.readbyqxmd.com/read/29036955/-comparision-of-risk-factors-and-pathogens-in-patients-with-early-and-late-onset-ventilator-associated-pneumonia-in-intensive-care-unit
#1
Y J Liang, Z L Li, L Wang, B Y Liu, R Y Ding, X C Ma
Objective: To compare risk factors and bacterial etiology in patients with early-onset versus late-onset ventilator-associated pneumonia (VAP) in intensive care unit (ICU). Methods: This prospective cohort study enrolled mechanically ventilated patients hospitalized for more than 48 hours in the first affiliated hospital, China Medical University from Jan 2012 to Jun 2016. Subjects were classified by ventilator status: early-onset VAP (< 5 d ventilation, E-VAP) or late-onset VAP (≥ 5 d ventilation, L-VAP)...
October 1, 2017: Zhonghua Nei Ke za Zhi [Chinese Journal of Internal Medicine]
https://www.readbyqxmd.com/read/29036914/molecular-characterization-and-functional-analysis-of-a-ferritin-heavy-chain-subunit-from-the-eri-silkworm-samia-cynthia-ricini
#2
Hai-Zhong Yu, Shang-Zhi Zhang, Yan Ma, Dong-Qiong Fei, Bing Li, Li-Ang Yang, Jie Wang, Zhen Li, Azharuddin Muhammad, Jia-Ping Xu
Ferritins are conserved iron-binding proteins that are primarily involved in iron storage, detoxification and the immune response. Despite the importance of ferritin in organisms, little is known about their roles in the eri-silkworm (Samia cynthia ricini). We previously identified a ferritin heavy chain subunit named ScFerHCH in the S. c. ricini transcriptome database. The full-length S. c. ricini ferritin heavy chain subunit (ScFerHCH) was 1863 bp and encoded a protein of 231 amino acids with a deduced molecular weight of 25...
October 14, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/29035674/the-startup-performance-and-microbial-distribution-of-an-anaerobic-baffled-reactor-abr-treating-medium-strength-synthetic-industrial-wastewater
#3
Hao Jiang, Hong Nie, Jiangtao Ding, Walter Stinner, Kaixuan Sun, Hongjun Zhou
In this study, an anaerobic baffled reactor (ABR) with seven chambers was applied to treat medium-strength synthetic industrial wastewater (MSIW). The performance of startup and shock test on treating MSIW was investigated. During the acclimation process, the chemical oxygen demand (COD) of MSIW gradually increased from 0 to 2,000 mg L(-1), and the COD removal finally reached 90%. At shock test, the feeding COD concentration increased by one-fifth and the reactor adapted very well with a COD removal of 82%...
October 16, 2017: Journal of Environmental Science and Health. Part A, Toxic/hazardous Substances & Environmental Engineering
https://www.readbyqxmd.com/read/29035090/early-respiratory-bacterial-detection-and-anti-staphylococcal-antibiotic-prophylaxis-in-young-children-with-cystic-fibrosis
#4
Matthew N Hurley, Andrew Fogarty, Tricia M McKeever, Christopher H Goss, Margaret Rosenfeld, Alan R Smyth
RATIONALE: Consensus is lacking regarding anti-staphylococcal antibiotic prophylaxis use for young children with cystic fibrosis. Prophylaxis is recommended in the UK, but recommended against in the US. OBJECTIVES: To test the hypothesis that anti-staphylococcal antibiotic prophylaxis is associated with a decreased risk of Staphylococcus aureus acquisition, but no increased risk of Pseudomonas aeruginosa acquisition. METHODS: We undertook a longitudinal observational study of children with cystic fibrosis who were recruited from birth (or their first registry entry in the period) and followed until the age of 4 years (1500 days) using UK CF Trust and US CF Foundation Registries, 2000-2009...
October 16, 2017: Annals of the American Thoracic Society
https://www.readbyqxmd.com/read/29035041/purification-and-characterization-of-a-novel-%C3%AE-cypermethrin-degrading-aminopeptidase-from-pseudomonas-aeruginosa-gf31
#5
Ai-Xing Tang, Hu Liu, You-Yan Liu, Qing-Yun Li, Yi-Ming Qing
In this study, a novel β-cypermethrin-degrading enzyme was isolated and purified by 32.8 fold from the extracellular cell-free filtrate of Pseudomonas aeruginosa GF31with the protein recovery of 26.6%. The molecular mass of the enzyme was determined to be 53 kDa. The optimum temperature for the activity was surprisingly 60 °C and moreover, the purified enzyme showed a good pH- stability, maintaining over 85% of its initial activity in the pH 5.0-9.0 range. Most of the common metal ions exhibited little influence on the activity except for Hg2+, Ag+ and Cu2+...
October 16, 2017: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/29034850/pseudomonas-aeruginosa-variants-obtained-from-veterinary-clinical-samples-reveal-a-role-for-cyclic-di-gmp-in-biofilm-formation-and-colony-morphology
#6
Maria T Brock, Galya C Fedderly, Grace I Borlee, Michael M Russell, Liliana K Filipowska, Doreene R Hyatt, Ryan A Ferris, Bradley R Borlee
Overuse of antibiotics is contributing to an emerging antimicrobial resistance crisis. To better understand how bacteria adapt tolerance and resist antibiotic treatment, Pseudomonas aeruginosa isolates obtained from infection sites sampled from companion animals were collected and evaluated for phenotypic differences. Selected pairs of clonal isolates were obtained from individual infection samples and were assessed for antibiotic susceptibility, cyclic di-GMP levels, biofilm production, motility and genetic-relatedness...
October 16, 2017: Microbiology
https://www.readbyqxmd.com/read/29034110/toll-like-receptor-4-dependent-inflammatory-responses-following-intestinal-colonization-of-secondary-abiotic-il10-deficient-mice-with-multidrug-resistant-pseudomonas-aeruginosa
#7
Anne Grunau, Ulrike Escher, Stefan Bereswill, Markus M Heimesaat
The rising incidences of infections with multidrug-resistant (MDR) Gram-negative bacteria including Pseudomonas aeruginosa (PA) have gained increasing attention in medicine, but also in the general public and global health politics. The mechanisms underlying opportunistic pathogen-host interactions are unclear, however. To address this, we challenged secondary abiotic IL10(-/-) mice deficient for Toll-like receptor-4 (TLR4(-/-) × IL10(-/-)), the main receptor of the Gram-negative cell wall constituent lipopolysaccharide, with a clinical MDR PA isolate...
September 2017: European Journal of Microbiology & Immunology
https://www.readbyqxmd.com/read/29034109/multidrug-resistant-pseudomonas-aeruginosa-induce-systemic-pro-inflammatory-immune-responses-in-colonized-mice
#8
Eliane von Klitzing, Stefan Bereswill, Markus M Heimesaat
The World Health Organization has rated multidrug-resistant (MDR) Pseudomonas aeruginosa as a critical threat to human health. In the present study, we performed a survey of intestinal colonization, and local and systemic immune responses following peroral association of secondary abiotic mice with either a clinical MDR P. aeruginosa or a commensal murine Escherichia coli isolate. Depletion of the intestinal microbiota following antibiotic treatment facilitated stable intestinal colonization of both P. aeruginosa and E...
September 2017: European Journal of Microbiology & Immunology
https://www.readbyqxmd.com/read/29034053/a-3d-printed-microbial-cell-culture-platform-with-in-situ-pegda-hydrogel-barriers-for-differential-substrate-delivery
#9
Andrea L Kadilak, Jessica C Rehaag, Cameron A Harrington, Leslie M Shor
Additive manufacturing, or 3D-printing techniques have recently begun to enable simpler, faster, and cheaper production of millifluidic devices at resolutions approaching 100-200 μm. At this resolution, cell culture devices can be constructed that more accurately replicate natural environments compared with conventional culturing techniques. A number of microfluidics researchers have begun incorporating additive manufacturing into their work, using 3D-printed devices in a wide array of chemical, fluidic, and even some biological applications...
September 2017: Biomicrofluidics
https://www.readbyqxmd.com/read/29033910/resistance-of-animal-strains-of-pseudomonas-aeruginosa-to-carbapenems
#10
Marisa Haenni, Maxime Bour, Pierre Châtre, Jean-Yves Madec, Patrick Plésiat, Katy Jeannot
Carbapenems are major antibiotics reserved to human medicine. This study aimed to investigate the mechanisms of carbapenem resistance of a selection of Pseudomonas aeruginosa veterinary strains from the French network Resapath. Thirty (5.7%) imipenem and/or meropenem non-susceptible P. aeruginosa of canine (n = 24), feline (n = 5), or bovine (n = 1) origin were identified in a large collection of 527 veterinary strains gathered by the Resapath. These resistant isolates belonged to 25 MultiLocus Sequence Types (MLST), of which 17 (68%) are shared with clinical (human) strains, such as high risk clones ST233 and ST395...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/29033902/analyzing-the-complex-regulatory-landscape-of-hfq-an-integrative-multi-omics-approach
#11
Lucia Grenga, Govind Chandra, Gerhard Saalbach, Carla V Galmozzi, Günter Kramer, Jacob G Malone
The ability of bacteria to respond to environmental change is based on the ability to coordinate, redirect and fine-tune their genetic repertoire as and when required. While we can learn a great deal from reductive analysis of individual pathways and global approaches to gene regulation, a deeper understanding of these complex signaling networks requires the simultaneous consideration of several regulatory layers at the genome scale. To highlight the power of this approach we analyzed the Hfq transcriptional/translational regulatory network in the model bacterium Pseudomonas fluorescens...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/29033561/specific-iga-against-pseudomonas-aeruginosa-in-severe-copd
#12
Laura Millares, Sara Martí, Carmen Ardanuy, Josefina Liñares, Salud Santos, Jordi Dorca, Marian García-Nuñez, Sara Quero, Eduard Monsó
BACKGROUND: The bronchial mucosa is protected by a specialized immune system focused on the prevention of colonization and infection by potentially pathogenic microorganisms (PPMs). Immunoglobulin A (IgA) is the principal antibody involved in this mechanism. A defective immune barrier may facilitate the recurrent presence of PPMs in COPD. PURPOSE: The aim of this study was to determine IgA-mediated bronchial specific immune responses against Pseudomonas aeruginosa in stable patients with severe disease...
2017: International Journal of Chronic Obstructive Pulmonary Disease
https://www.readbyqxmd.com/read/29033518/a-rare-experience-of-infective-residual-sheath-in-the-wall-of-the-innominate-vein-after-extraction-of-implantable-defibrillators
#13
Yuan-Hao Liu, Wei-Shiang Lin, Yi-Chang Lin, Chih-Yuan Lin, Chien-Sung Tsai, Yi-Ting Tsai
BACKGROUND: Cardiac device-related infective endocarditis is an uncommon but potentially fatal complication. Therefore, cardiac devices should be removed as soon as a device-related infection is suspected. CASE REPORT: A 56-year-old male with a history of arrhythmogenic right ventricular dysplasia with implantable cardioverter-defibrillators (ICDs) 7 years earlier and re-implantation of ICDs due to dysfunction 18 months ago presented with erosion of the ICD pocket with Pseudomonas bacteremia...
July 2017: Acta Cardiologica Sinica
https://www.readbyqxmd.com/read/29033313/developing-a-model-for-cystic-fibrosis-sociomicrobiology-based-on-antibiotic-and-environmental-stress
#14
Susana Patrícia Lopes, Nuno Filipe Azevedo, Maria Olívia Pereira
Cystic fibrosis (CF) infections are invariably biofilm-mediated and polymicrobial, being safe to assume that a myriad of factors affects the sociomicrobiology within the CF infection site and modulate the CF community dynamics, by shaping their social activities, overall functions, virulence, ultimately affecting disease outcome. This work aimed to assess changes in the dynamics (particularly on the microbial composition) of dual-/three-species biofilms involving CF-classical (Pseudomonas aeruginosa) and unusual species (Inquilinus limosus and Dolosigranulum pigrum), according to variable oxygen conditions and antibiotic exposure...
September 28, 2017: International Journal of Medical Microbiology: IJMM
https://www.readbyqxmd.com/read/29033275/mechanisms-of-humoral-immune-response-against-pseudomonas-aeruginosa-biofilm-infection-in-cystic-fibrosis
#15
REVIEW
Renan Marrichi Mauch, Peter Østrup Jensen, Claus Moser, Carlos Emilio Levy, Niels Høiby
P. aeruginosa chronic lung infection is the major cause of morbidity and mortality in patients with cystic fibrosis (CF), and is characterized by a biofilm mode of growth, increased levels of specific IgG antibodies and immune complex formation. However, despite being designed to combat this infection, such elevated humoral response is not associated with clinical improvement, pointing to a lack of anti-pseudomonas effectiveness. The mode of action of specific antibodies, as well as their structural features, and even the background involving B-cell production, stimulation and differentiation into antibody-producing cells in the CF airways are poorly understood...
October 12, 2017: Journal of Cystic Fibrosis: Official Journal of the European Cystic Fibrosis Society
https://www.readbyqxmd.com/read/29033215/treating-resistant-pseudomonas-aeruginosa-lung-disease-in-young-children-with-cystic-fibrosis
#16
REVIEW
Sandy Z P Lim, Dominic A Fitzgerald
Pseudomonas aeruginosa is a common bacterial pathogen in the evolution of bronchiectasis in cystic fibrosis. The appearance of resistant strains of pseudomonas is increasing with the earlier and more liberal use of a range of anti-pseudomonal antibiotics for the treatment of bacterial chest infections. The rationale for treatment and potential benefits of aggressive treatment of resistant strains of Pseudomonas aeruginosa from early in life are discussed.
September 1, 2017: Paediatric Respiratory Reviews
https://www.readbyqxmd.com/read/29033110/investigating-of-four-main-carbapenem-resistance-mechanisms-in-high-level-carbapenem-resistant-pseudomonas-aeruginosa-isolated-from-burn-patients
#17
Soodabeh Rostami, Ahmad Farajzadeh Sheikh, Saeed Shoja, Abbas Farahani, Mohammad Amin Tabatabaiefar, Abbas Jolodar, Raheleh Sheikhi
BACKGROUND: Pseudomonas aeruginosa is an opportunistic pathogen involved in many infections. Carbapenem-resistant P.aeruginosa has emerged as an important cause of infection in different hospitals worldwide. We aimed to determine frequencies of the four main resistance mechanisms [metallo-beta lactamase (MBL) production (blaIMP, blaVIM, blaSPM and blaNDM), overproduction of the MexAB-OprM and MexXY efflux pumps, overproduction of chromosome-encoded AmpC β-lactamase, and reduced OprD expression] in high-level carbapenem-resistant P...
October 9, 2017: Journal of the Chinese Medical Association: JCMA
https://www.readbyqxmd.com/read/29033074/potentially-active-spoilage-bacteria-community-during-the-storage-of-vacuum-packaged-beefsteaks-treated-with-aqueous-ozone-and-electrolyzed-water
#18
Cristian Botta, Ilario Ferrocino, Maria Chiara Cavallero, Simonetta Riva, Manuela Giordano, Luca Cocolin
The microbial contamination that occurs during the slaughtering process and during handling of the meat results in a shortening of the shelf-life of meat. In this study, which has had the aim of extending the shelf life of beefsteaks, pilot-scale treatments were carried out with aqueous ozone (AO) and electrolyzed water (EW) before vacuum packaging (VP). The development of the potentially active microbiota and the associated volatilome were followed over 15days of storage under refrigerated conditions (4°C), in order to define the potential long-term effects of the treatments and storage condition on microbiota...
October 7, 2017: International Journal of Food Microbiology
https://www.readbyqxmd.com/read/29032906/live-dead-discrimination-analysis-qpcr-assessment-for-opportunistic-pathogens-and-population-analysis-at-ozone-wastewater-treatment-plants
#19
Thomas Jäger, Johannes Alexander, Silke Kirchen, Andreas Dötsch, Arne Wieland, Christian Hiller, Thomas Schwartz
In respect to direct and indirect water reuse, the microbiological quality of treated wastewater is highly important. Conventional wastewater treatment plants are normally not equipped with advanced technologies for the elimination of bacteria. Molecular biology analyses were combined with live-dead discrimination analysis of wastewater population using Propidium monoazide (PMA) to study population shifts during ozonation (1 g ozone/g DOC) at a municipal wastewater treatment plant. Escherichia coli, enterococci, and Pseudomonas aeruginosa were quantified by polymerase chain reaction (qPCR) and the whole wastewater population was analyzed by metagenomic sequencing...
October 9, 2017: Environmental Pollution
https://www.readbyqxmd.com/read/29032644/characterization-of-bacterial-community-dynamics-during-the-decomposition-of-pig-carcasses-in-simulated-soil-burial-and-composting-systems
#20
Bo-Min Ki, Yu Mi Kim, Jun Min Jeon, Hee Wook Ryu, Kyung-Suk Cho
Soil burial is the most widely used disposal method for infected pig carcasses, but composting has gained attention as an alternative disposal method because pig carcasses can be decomposed rapidly and safely by composting. To understand the pig carcass decomposition process in soil burial and by composting, pilot-scale test systems that simulated soil burial and composting were designed and constructed in field. The envelope material samples were collected using special sampling devices without disturbance, and bacterial community dynamics were analyzed by high-throughput pyrosequencing for 340 days...
October 14, 2017: Journal of Microbiology and Biotechnology
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