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Maiara Brusco de Freitas, Emilia Addison Machado Moreira, Camila Tomio, Yara Maria Franco Moreno, Felipe Perozzo Daltoe, Eliana Barbosa, Norberto Ludwig Neto, Vittoria Buccigrossi, Alfredo Guarino
The aim of the present study was to evaluate the effect of cystic fibrosis and antibiotic therapy on intestinal microbiota composition and intestinal inflammation in children and adolescents. A cross-sectional controlled study was conducted with 36 children and adolescents: 19 in the cystic fibrosis group (CFG) and 17 in the control group (CG) matched for age and sex. The CFG was subdivided based on the use of antibiotic therapy (CFAB group) and non-use of antibiotic therapy (CFnAB group). The following data were evaluated: colonization, antibiotic therapy, mutation, breastfeeding, use of infant formula, type of delivery, introduction of solid foods, body mass index, fecal calprotectin and intestinal microbiota composition (fluorescence in situ hybridization)...
2018: PloS One
Gayan Senavirathne, Miguel A Lopez, Ryan Messer, Richard Fishel, Kristine E Yoder
Single-molecule (SM) microscopy is a powerful tool capable of visualizing individual molecules and events in real time. SM imaging may rely on proteins or nucleic acids labelled with a fluorophore. Unfortunately photobleaching of fluorophores leads to irreversible loss of signal, impacting the collection of data from SM experiments. Trace amounts of dissolved oxygen (O2 ) are the main cause of photobleaching. Oxygen scavenging systems (OSS) have been developed that decrease dissolved O2 . Commercial OSS enzyme preparations are frequently contaminated with nucleases that damage nucleic acid substrates...
June 19, 2018: Analytical Biochemistry
Hae Ja Shin, Woon Ki Lim
In efforts to speed up the assessment of microorganisms, researchers have sought to use bacteriophages as a biosensing tool, due to their host-specificity, wide abundance, and safety. However, the lytic cycle of the phage has limited its efficacy as a biosensor. Here, we cloned a fragment of tail protein J from phage lambda and characterized its binding with the host, E. coli K-12, and other microorganism. The N-terminus of J was fused with a His-tag (6HN-J), overexpressed, purified, and characterized using anti-His monoclonal antibodies...
June 22, 2018: Preparative Biochemistry & Biotechnology
Dandan Pan, Mengmeng Sun, Yawen Wang, Pei Lv, Xiangwei Wu, Qing X Li, Haiqun Cao, Rimao Hua
Nicotine is a major toxic alkaloid in wastes generated from tobacco production and cigarette manufacturing. In the present work, a nicotine-degrading bacterial strain was isolated from tobacco powdery waste. The isolate was identified as Pseudomonas sp. S-1 based on morphology, physiology, and 16S rRNA gene sequence. Suitable conditions of isolate S-1 for nicotine degradation were pH 7.0 and 30 C. Catabolic intermediates of nicotine were isolated with preparative-HPLC and characterized with LC-HRMS and NMR...
June 22, 2018: Journal of Agricultural and Food Chemistry
Xuan Yang, Ronald Domalaon, Yinfeng Lyu, George G Zhanel, Frank Schweizer
In this study, we examined the in vitro effect of tobramycin-efflux pump inhibitor (TOB-EPI) conjugates in combinations with fluoroquinolones, rifampicin and fosfomycin on the growth of multi-drug resistant (MDR) and extremely-drug resistant (XDR) Pseudomonas aeruginosa . The TOB-EPI conjugates include tobramycin covalently linked to 1-(1-naphthylmethyl)-piperazine (NMP) ( 1 ), paroxetine (PAR) ( 2 ) and a dibasic peptide analogue of MC-04,124 (DBP) ( 3 ). Potent synergism was found for combinations of TOB-NMP ( 1 ), TOB-PAR ( 2 ) or TOB-DBP ( 3 ) with either fluoroquinolones (moxifloxacin, ciprofloxacin), rifampicin or fosfomycin against a panel of multidrug-resistant/extensively drug-resistant (MDR/XDR) P...
June 22, 2018: Journal of Clinical Medicine
Dimitrios Papadopoulos, Iosif Bader, Efthalia Gkioxari, Vasiliki Petta, Theodoros Tsaras, Nikoletta Galanopoulou, Maria-Anna Archontouli, Filia Diamantea, Emmanouil Kastanakis, Napoleon Karagianidis, Vasiliki Filaditaki
Aspiration pneumonia has a high incidence in hospitalized patients with community-acquired pneumonia and results in high mortality rates. We aimed to evaluate microbiology and assess prognostic factors of aspiration pneumonia in the setting of a tertiary hospital pulmonology department. Community-acquired (CAAP) and healthcare-associated aspiration pneumonia (HCAAP) cases hospitalized over a period of a year were prospectively followed. Demographic, clinical, biological and radiological data were recorded at admission, while sputum, tracheal aspirates or bronchial washing samples were collected within 48 hours of admission...
June 1, 2018: Le Infezioni in Medicina
Shiyin Liu, Fei He, Nuoqiao Lin, Yumei Chen, Zhibin Liang, Lisheng Liao, Mingfa Lv, Yufan Chen, Shaohua Chen, Jianuan Zhou, Lian-Hui Zhang
Sexual mating of compatible sporida is essential for Sporisorium scitamineum to form dikaryotic mycelia and then cause infection on sugarcane. Our previous work identified a Pseudomonas sp. ST4 from a soil sample, which showed a promising biocontrol potential by inhibiting the mating of S. scitamineum sporida and hyphal growth. In this study, we set to isolate the active compounds from Pseudomonas sp. ST4 through solid fermentation. High-performance liquid chromatography (HPLC) separation coupling with bioassay showed that Pseudomonas sp...
June 21, 2018: Microbial Biotechnology
Claire Bernardin-Souibgui, Sylvie Barraud, Emilie Bourgeois, Jean-Baptiste Aubin, Celine Becouze-Lareure, Laure Wiest, Laurence Marjolet, Celine Colinon, Ghislain Lipeme Kouyi, Benoit Cournoyer, Didier Blaha
The nature and fate of urban contaminants washed by stormwater events and accumulating in a detention basin (DB) were investigated. Relations between bacterial and chemical contaminants of trapped urban sediments, and field parameters were analyzed. Fecal indicators and some pathogens known to be environmentally transmitted (Nocardia, Pseudomonas aeruginosa, and Aeromonas caviae) were tracked, and their persistence investigated. Six sampling campaigns were carried out over 3 years, using five sites including a settling chamber (SC)...
June 21, 2018: Environmental Science and Pollution Research International
Panisa Treepong, Christophe Guyeux, Alexandre Meunier, Charlotte Couchoud, Didier Hocquet, Benoit Valot
Motivation: The advent of next-generation sequencing has boosted the analysis of bacterial genome evolution. Insertion sequence (IS) elements play a key role in prokaryotic genome organization and evolution, but their repetitions in genomes complicate their detection from short-read data. Results: PanISa is a software pipeline that identifies IS insertions ab initio in bacterial genomes from short-read data. It is a highly sensitive and precise tool based on the detection of read-mapping patterns at the insertion site...
June 19, 2018: Bioinformatics
Fan Zhao, Lina Niu, Jinqing Nong, Chunmei Wang, Jing Wang, Yan Liu, Naishu Gao, Xiaoxue Zhu, Lei Wu, Shoukui Hu
Pseudomonas aeruginosa causes nosocomial infections of burn patients and other immunocompromised individuals, but the conventional diagnosis of P. aeruginosa infection depends on time-consuming culture-based methods. Hence, a simple, fast, sensitive technique for detection of P. aeruginosa using multiple cross displacement amplification (MCDA) and gold nanoparticle-based lateral flow biosensors (LFB) was developed. By using this technique, the reaction could be completed at an optimized constant temperature (67°C) within only 40 min...
June 19, 2018: FEMS Microbiology Letters
Ashkan Afshari, Lyly Nguyen, Steven A Kahn, Amelia C Montgomery, Takashi Shinha, Charles Stratton, Blair Summitt
Mafenide acetate is an effective but costly antimicrobial solution used for burn wounds. The package insert instructs the user to discard unused solution within 48 hours of opening. The purpose of this study is to evaluate the antimicrobial activity of mafenide acetate beyond 48 hours after reconstitution, to possibly reduce cost by eliminating product waste. Staphylococcus aureus and Pseudomonas aeruginosa isolates were used to seed Mueller-Hinton agar plates. Filter paper disks were then saturated with 5% mafenide acetate at 0, 2, 7, 14, 30, and 60 days after reconstitution...
February 17, 2018: Journal of Burn Care & Research: Official Publication of the American Burn Association
Taylor E Woo, Rachel Lim, Michael G Surette, Barbara Waddell, Joel C Bowron, Ranjani Somayaji, Jessica Duong, Christopher H Mody, Harvey R Rabin, Douglas G Storey, Michael D Parkins
The natural history and epidemiology of Pseudomonas aeruginosa infections in non-cystic fibrosis (non-CF) bronchiectasis is not well understood. As such it was our intention to determine the evolution of airway infection and the transmission potential of P. aeruginosa in patients with non-CF bronchiectasis. A longitudinal cohort study was conducted from 1986-2011 using a biobank of prospectively collected isolates from patients with non-CF bronchiectasis. Patients included were ≥18 years old and had ≥2 positive P...
April 2018: ERJ Open Research
Rashmi Kurli, Diptaraj Chaudhari, Aabeejjeet N Pansare, Mitesh Khairnar, Yogesh S Shouche, Praveen Rahi
A substantial majority of global population owns cellular phones independently to demographic factors like age, economic status, and educational attainment. In this study, we investigated the diversity of microorganisms associated with cellular phones of 27 individuals using cultivation-based methods. Cellular phones were sampled using cotton swabs and a total of 554 isolates representing different morphotypes were obtained on four growth media. Matrix-assisted laser desorption ionization time of flight (MALDI-TOF) mass spectrometry could generate protein profiles for 527 isolates and species-level identification was obtained for 415 isolates...
2018: Frontiers in Microbiology
Mohammed Ashrafi, Lilyann Novak-Frazer, Matthew Bates, Mohamed Baguneid, Teresa Alonso-Rasgado, Guoqing Xia, Riina Rautemaa-Richardson, Ardeshir Bayat
Biofilms are major contributors to delayed wound healing and there is a need for clinically relevant experimental models to assess theranostics. Microorganisms release volatile organic compounds (VOCs) and the ability to identify these in infected cutaneous wounds could lead to efficient non-invasive diagnosis. The aims here were to develop and assess bacterial biofilm formation and identify their VOC profiles in an in vitro model and validate in human ex vivo incisional and excisional cutaneous wound models...
June 21, 2018: Scientific Reports
Ashish Pathak, Rajneesh Jaswal, Paul Stothard, Scott Brooks, Ashvini Chauhan
The draft genome sequence of Pseudomonas sp. strain B1, isolated from a contaminated soil, is reported. The genome comprises 6,706,934 bases, 6,059 coding sequences, and 70 RNAs and has a G+C content of 60.3%. A suite of biodegradative genes, many located on genomic islands, were identified from strain B1, further enhancing our understanding of the versatile pseudomonads.
June 21, 2018: Genome Announcements
Jordan Vacheron, Peter Kupferschmied, Grégory Resch, Christoph Keel
We report here the complete annotated genome sequence of ΦGP100, a lytic bacteriophage of the Podoviridae family. ΦGP100 was isolated from rhizosphere soil in Switzerland and infects specifically strains of Pseudomonas protegens that are known for their plant-beneficial activities. Phage ΦGP100 has a 50,547-bp genome with 76 predicted open reading frames.
June 21, 2018: Genome Announcements
Jeremy S Morris, Ryan A Groves, Jillian M Hagel, Peter J Facchini
Phenylalkylamines, such as the plant compounds ephedrine and pseudoephedrine and the animal neurotransmitters dopamine and adrenaline, compose a large class of natural and synthetic molecules with important physiological functions and pharmaceutically valuable bioactivities. The final steps of ephedrine and pseudoephedrine biosynthesis in members of the plant genus Ephedra involve N -methylation of norephedrine and norpseudoephedrine, respectively. Here, using a plant transcriptome screen, we report the isolation and characterization of an N -methyltransferase (NMT) from Ephedra sinica able to catalyze the formation of (pseudo)ephedrine and other naturally occurring phenylalkylamines, including N -methylcathinone and N -methyl(pseudo)ephedrine...
June 21, 2018: Journal of Biological Chemistry
Uttariya Roy, Shubhalakshmi Sengupta, Priya Banerjee, Papita Das, Avijit Bhowal, Siddhartha Datta
This study focuses on the investigation of removal of textile dye (Reactive Yellow) by a combined approach of sorption integrated with biodegradation using low cost adsorbent fly ash immobilized with Pseudomonas sp. To ensure immobilization of bacterial species on treated fly ash, fly ash with immobilized bacterial cells was characterized using Fourier transform infrared (FTIR) spectroscopy, scanning electron microscopy (SEM), and fluorescence microscopy. Comparative batch studies were carried out using Pseudomonas sp, fly ash and immobilized Pseudomonas sp on flyash and were observed that immobilized Pseudomonas sp on flyash acted as better decolourizing agent...
June 18, 2018: Journal of Environmental Management
Evelina L Zdorovenko, Alexander S Shashkov, Alexandra A Kadykova, Elena P Kiseleva, Victoria V Savich, Galina I Novik, Yuriy A Knirel
Strain of Pseudomonas psychrotolerans was cultured on the nutrient agar and in a liquid nutrient broth. Bacterial cells were phage-typed with bacteriophages specific to Pseudomonas. O-antigen was isolated from cells using phenol-water method and mild acid degradation. The following structures of the polysaccharides extracted were established by sugar analysis and 1D, 2D NMR spectroscopy: PSI→3)-α-D-Manp-(1→2)-α-D-Manp-(1→; PSII→3)-α-D-Rhap-(1→2)-β-D-Rhap-(1→3)-α-D-Rhap-(1→; α-D-Glcp-(1˩; 2...
June 18, 2018: Carbohydrate Research
Francine Tavares da Silva, Kamila Furtado da Cunha, Laura Martins Fonseca, Mariana Dias Antunes, Shanise Lisie Mello El Halal, Ângela Maria Fiorentin, Elessandra da Rosa Zavareze, Alvaro Renato Guerra Dias
The ultrafine fibers were produced using a polymeric blend of soy protein isolate (SPI), polyethylene oxide (PEO), and zein at a ratio of 1:1:1 (v/v/v) by electrospinning. The ginger essential oil (GEO) was encapsulated in the ultrafine fibers and the morphology, Fourier-Transform Infrared Spectroscopy (FTIR) analysis, thermal properties and relative crystallinity were evaluated. The antimicrobial activity of ginger essential oil was evaluated against five bacteria (Listeria monocytogenes, Staphylococcus aureus, Escherichia coli 0157:H7, Salmonella typhimurium, and Pseudomonas aeruginosa)...
June 18, 2018: International Journal of Biological Macromolecules
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