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https://www.readbyqxmd.com/read/29471305/humanized-exposures-of-cefiderocol-a-siderophore-cephalosporin-display-sustained-in-vivo-activity-against-siderophore-resistant-pseudomonas-aeruginosa
#1
Islam M Ghazi, Marguerite L Monogue, Masakatsu Tsuji, David P Nicolau
We evaluated the in vivo efficacy of humanized exposures of cefiderocol, a novel siderophore cephalosporin, against a test panel of P. aeruginosa (PSA) previously shown to develop resistance to 2 preclinical candidate siderophores (MB-1 and SMC-3176). In the thigh infection model, the PSA bacterial density in untreated controls grew from 5.54 ± 0.23 to 8.68 ± 0.57 log10 CFU over 24 h. The humanized cefiderocol exposure resulted in >1 log10 CFU reduction in all 8 isolates, while MB-1 and SMC-3176 exhibited variable activity similar to that previously reported...
February 22, 2018: Pharmacology
https://www.readbyqxmd.com/read/29471265/role-of-rpos-in-stress-resistance-quorum-sensing-and-spoilage-potential-of-pseudomonas-fluorescens
#2
Xiaoxiang Liu, Lei Ji, Xu Wang, Jianrong Li, Junli Zhu, Aihua Sun
Microorganism activities are considered the main cause of most food spoilage, leading to great economic losses. Pseudomonas fluorescens is a Gram-negative bacterium that is widely found in food and has high spoilage activity. RpoS is considered an important global regulator involved in stress survival and virulence in many pathogens. Thus, it is very possible that RpoS plays an important role in spoilage regulation in P. fluorescens. In this study an in-frame deletion mutation of rpoS was constructed to explore its function in P...
February 14, 2018: International Journal of Food Microbiology
https://www.readbyqxmd.com/read/29471219/the-effects-of-extrinsic-factors-on-the-structural-and-mechanical-properties-of-pseudomonas-fluorescens-biofilms-a-combined-study-of-nutrient-concentrations-and-shear-conditions
#3
Ashley Allen, Olivier Habimana, Eoin Casey
The growth of biofilms on surfaces is a complicated process influenced by several environmental factors such as nutrient availability and fluid shear. In this study, combinations of growth conditions were selected for the study of Pseudomonas fluorescens biofilms including as cultivation time (24- or 48 h), nutrient levels (1:1 or 1:10 King B medium), and shear conditions (75 RPM shaking, 0.4 mL min-1 or 0.7 mL min-1 ). The use of Confocal Laser Scanning Microscopy (CLSM) determined biofilm structure, while liquid-phase Atomic Force Microscopy (AFM) techniques resolved the mechanical properties of biofilms...
February 15, 2018: Colloids and Surfaces. B, Biointerfaces
https://www.readbyqxmd.com/read/29471126/adsorption-mechanism-of-extracellular-polymeric-substances-from-two-bacteria-on-ultisol-and-alfisol
#4
Li-Ying Ren, Zhi-Neng Hong, Wei Qian, Jiu-Yu Li, Ren-Kou Xu
The primary objective of this study was to identify the capacity and mechanism of extracellular polymeric substance (EPS) adsorption on soil colloids of Alfisol and Ultisol at different pH and ionic strengths. Two kinds of EPS were extracted from Bacillus subtilis and Pseudomonas fluorescens by centrifugation, and their adsorption on Ultisol and Alfisol was investigated using a batch adsorption experiment and attenuated total reflectance-Fourier transform infrared spectroscopy (ATR-FTIR). The average diameter of EPS from B...
February 19, 2018: Environmental Pollution
https://www.readbyqxmd.com/read/29471025/releasing-iron-from-ferritin-protein-nanocage-by-reductive-method-the-role-of-electron-transfer-mediator
#5
Prashanth Kumar Koochana, Abhinav Mohanty, Suman Das, Biswamaitree Subhadarshanee, Suresh Satpati, Anshuman Dixit, Surendra Chandra Sabat, Rabindra K Behera
BACKGROUND: Ferritin detoxifies excess of free Fe(II) and concentrates it in the form of ferrihydrite (Fe2 O3 ·xH2 O) mineral. When in need, ferritin iron is released for cellular metabolic activities. However, the low solubility of Fe(III) at neutral pH, its encapsulation by stable protein nanocage and presence of dissolved O2 limits in vitro ferritin iron release. METHODS: Physiological reducing agent, NADH (E1/2  = -330 mV) was inefficient in releasing ferritin iron (E1/2  = +183 mV), when used alone...
February 19, 2018: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/29470920/long-term-microevolution-of-pseudomonas-aeruginosa-differs-between-mildly-and-severely-affected-cystic-fibrosis-lungs
#6
Jens Klockgether, Nina Cramer, Sebastian Fischer, Lutz Wiehlmann, Burkhard Tümmler
RATIONALE: The chronic airway infections with Pseudomonas aeruginosa determine morbidity in most individuals with cystic fibrosis (CF). P. aeruginosa may persist for decades in CF lungs which provides the rare opportunity to study the long-term within-host evolution of a bacterial airway pathogen. OBJECTIVES: To resolve the genetic adaptation of P. aeruginosa in CF lungs from the onset of colonization until the patient's death or permanent replacement by another P...
February 22, 2018: American Journal of Respiratory Cell and Molecular Biology
https://www.readbyqxmd.com/read/29470663/miniaturized-dispersive-liquid-liquid-microextraction-and-maldi-ms-using-ionic-liquid-matrices-for-the-detection-of-bacterial-communication-molecules-and-virulence-factors
#7
Jan Leipert, Ingrid Bobis, Sabine Schubert, Helmut Fickenscher, Matthias Leippe, Andreas Tholey
The identification and quantification of molecules involved in bacterial communication are major prerequisites for the understanding of interspecies interactions at the molecular level. We developed a procedure allowing the determination of 2-heptyl-4(1H)-quinolone (HHQ) and 2-heptyl-3-hydroxy-4(1H)-quinolone (PQS) and the virulence factor pyocyanin (PYO) formed by the Gram-negative bacterium Pseudomonas aeruginosa. The method is based on dispersive liquid-liquid microextraction from small supernatant volumes (below 10 μL) followed by quantitative matrix-assisted laser desorption/ionization (MALDI) mass spectrometry (MS)...
February 22, 2018: Analytical and Bioanalytical Chemistry
https://www.readbyqxmd.com/read/29470531/epidemiology-and-risk-factors-of-extensively-drug-resistant-pseudomonas-aeruginosa-infections
#8
Nattawan Palavutitotai, Anupop Jitmuang, Sasima Tongsai, Pattarachai Kiratisin, Nasikarn Angkasekwinai
BACKGROUND: The incidence of nosocomial infections from extensively drug-resistant Pseudomonas aeruginosa (XDR-PA) has been increasing worldwide. We investigated the prevalence and factors associated with XDR-PA infections, including the factors that predict mortality. METHODS: We retrospectively studied a cohort of adult, hospitalized patients with P. aeruginosa (PA) infections between April and December 2014. RESULTS: Of the 255 patients with PA infections, 56 (22%) were due to XDR-PA, 32 (12...
2018: PloS One
https://www.readbyqxmd.com/read/29470429/belowground-interactions-impact-the-soil-bacterial-community-soil-fertility-and-crop-yield-in-maize-peanut-intercropping-systems
#9
Qisong Li, Jun Chen, Linkun Wu, Xiaomian Luo, Na Li, Yasir Arafat, Sheng Lin, Wenxiong Lin
Intercropping has been widely used to control disease and improve yield in agriculture. In this study, maize and peanut were used for non-separation intercropping (NS), semi-separation intercropping (SS) using a nylon net, and complete separation intercropping (CS) using a plastic sheet. In field experiments, two-year land equivalent ratios (LERs) showed yield advantages due to belowground interactions when using NS and SS patterns as compared to monoculture. In contrast, intercropping without belowground interactions (CS) showed a yield disadvantage...
February 22, 2018: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/29470394/a-novel-early-warning-system-based-on-a-sediment-microbial-fuel-cell-for-in-situ-and-real-time-hexavalent-chromium-detection-in-industrial-wastewater
#10
Shuai Zhao, Pu Liu, Yongyan Niu, Zhengjun Chen, Aman Khan, Pengyun Zhang, Xiangkai Li
Hexavalent chromium (Cr(VI)) is a well-known toxic heavy metal in industrial wastewater, but in situ and real time monitoring cannot be achieved by current methods used during industrial wastewater treatment processes. In this study, a Sediment Microbial Fuel Cell (SMFC) was used as a biosensor for in situ real-time monitoring of Cr(VI), which was the organic substrate is oxidized in the anode and Cr(VI) is reduced at the cathode simultaneously. The pH 6.4 and temperature 25 °C were optimal conditions for the operation...
February 22, 2018: Sensors
https://www.readbyqxmd.com/read/29470048/designing-porphyrinic-covalent-organic-frameworks-for-the-photodynamic-inactivation-of-bacteria
#11
Jan Hynek, Jaroslav Zelenka, Jiri Rathousky, Pavel Kubát, Tomáš Ruml, Jan Demel, Kamil Lang
Microbial colonization of biomedical devices is a recognized complication contributing to healthcare-associated infections. One of the possible approaches to prevent surfaces from the biofilm formation is antimicrobial photodynamic inactivation, based on the cytotoxic effect of singlet oxygen, O2 (1 Δg ), a short-lived, highly oxidative species, produced by energy transfer between excited photosensitizers and molecular oxygen. We synthesized porphyrin-based covalent organic frameworks (COFs) by the Schiff-base chemistry...
February 22, 2018: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/29469562/surface-topography-hinders-bacterial-surface-motility
#12
Yow-Ren Chang, Eric R Weeks, William A Ducker
We demonstrate that the surface motility of the bacterium, Pseudomonas aeruginosa, is hindered by a crystalline hemispherical topography with wavelength in the range 2-8 µm. The motility was determined by analysis of time-lapse microscopy images of cells in flowing growth medium maintained at 37°C. The net displacement of bacteria over 5 minutes is much lower on surfaces containing 2-8 µm hemispheres than on flat topography, but displacement on the 1 µm hemispheres is not lower. That is, there is a threshold between 1 µm and 2 µm for response to the topography...
February 22, 2018: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/29468490/negative-pressure-wound-therapy-reduces-the-motility-of-pseudomonas-aeruginosa-and-enhances-wound-healing-in-a-rabbit-ear-biofilm-infection-model
#13
Wang Guoqi, Li Zhirui, Wang Song, Li Tongtong, Zhang Lihai, Zhang Licheng, Tang Peifu
Pseudomonas aeruginosa motility, virulence factors and biofilms are known to be detrimental to wound healing. The efficacy of negative pressure wound therapy (NPWT) against P. aeruginosa has been little studied, either in vitro or in vivo. The present study evaluated the effect of negative pressure (NP) on P. aeruginosa motility in vitro, and the effect of NPWT on virulence factors and biofilms in vivo. P. aeruginosa motility was quantified under different levels of NP (atmospheric pressure, - 75, - 125, - 200 mmHg) using an in vitro model...
February 21, 2018: Antonie Van Leeuwenhoek
https://www.readbyqxmd.com/read/29468129/conversion-and-assimilation-of-furfural-and-5-hydroxymethyl-furfural-by-pseudomonas-putida-kt2440
#14
Michael T Guarnieri, Mary Ann Franden, Christopher W Johnson, Gregg T Beckham
The sugar dehydration products, furfural and 5-(hydroxymethyl)furfural (HMF), are commonly formed during high-temperature processing of lignocellulose, most often in thermochemical pretreatment, liquefaction, or pyrolysis. Typically, these two aldehydes are considered major inhibitors in microbial conversion processes. Many microbes can convert these compounds to their less toxic, dead-end alcohol counterparts, furfuryl alcohol and 5-(hydroxymethyl)furfuryl alcohol. Recently, the genes responsible for aerobic catabolism of furfural and HMF were discovered in Cupriavidus basilensis HMF14 to enable complete conversion of these compounds to the TCA cycle intermediate, 2-oxo-glutarate...
June 2017: Metabolic Engineering Communications
https://www.readbyqxmd.com/read/29467721/a-microfluidics-and-agent-based-modeling-framework-for-investigating-spatial-organization-in-bacterial-colonies-the-case-of-pseudomonas-aeruginosa-and-h1-type-vi-secretion-interactions
#15
Jared L Wilmoth, Peter W Doak, Andrea Timm, Michelle Halsted, John D Anderson, Marta Ginovart, Clara Prats, Xavier Portell, Scott T Retterer, Miguel Fuentes-Cabrera
The factors leading to changes in the organization of microbial assemblages at fine spatial scales are not well characterized or understood. However, they are expected to guide the succession of community development and function toward specific outcomes that could impact human health and the environment. In this study, we put forward a combined experimental and agent-based modeling framework and use it to interpret unique spatial organization patterns of H1-Type VI secretion system (T6SS) mutants of P . aeruginosa under spatial confinement...
2018: Frontiers in Microbiology
https://www.readbyqxmd.com/read/29467483/clove-bud-oil-modulates-pathogenicity-phenotypes-of-the-opportunistic-human-pathogen-pseudomonas-aeruginosa
#16
Jayalekshmi Haripriyan, Athira Omanakuttan, Nitasha D Menon, Muralidharan Vanuopadath, Sudarslal Sadasivan Nair, Ross Corriden, Bipin G Nair, Victor Nizet, Geetha B Kumar
Earlier studies from our laboratory have demonstrated that clove bud oil (CBO) attenuates expression of certain virulence factors of Pseudomonas aeruginosa PAO1. Here, we probe more deeply into the effect of CBO on four pseudomonal proteases - elastase A, elastase B, protease IV and alkaline protease - each known to play key roles in disease pathogenesis. CBO inhibited the activity of these proteases present in the bacterial culture supernatant. Zymography studies indicated that these proteases can activate host matrix metalloproteases (MMPs) to establish infection, through conversion of pro-MMP-2 to active MMP-2...
February 21, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29466772/effects-of-fe-s-as-coupled-redox-processes-on-arsenic-mobilization-in-shallow-aquifers-of-datong-basin-northern-china
#17
Junwen Zhang, Teng Ma, Yani Yan, Xianjun Xie, Olusegun K Abass, Congqiang Liu, Zhiqi Zhao, Zhizhen Wang
High arsenic groundwater generally coexists with elevated Fe2+ concentrations (mg L-1 levels) under reducing conditions, but an explanation for the extremely high arsenic (up to ∼2690) concentrations at very low Fe2+ (i.e., μg L-1 levels) in groundwater of Datong Basin remains elusive. Field groundwater investigation and laboratory microcosm experiments were implemented in this study. The field groundwater was characterized by weakly alkaline (pH 7.69 to 8.34) and reducing conditions (Eh -221.7 to -31...
February 18, 2018: Environmental Pollution
https://www.readbyqxmd.com/read/29466659/soil-bacteria-override-speciation-effects-on-zinc-phytotoxicity-in-zinc-contaminated-soils
#18
Nyekachi Adele, Bryne Ngwenya, Kate Victoria Heal, J Frederick Willem Mosselmans
The effects of zinc (Zn) speciation on plant growth in Zn-contaminated soil in the presence of bacteria are unknown but are critical to our understanding of metal biodynamics in the rhizosphere where bacteria are abundant. A 6-week pot experiment investigated the effects of two plant growth promoting bacteria (PGPB), Rhizobium leguminosarum and Pseudomonas brassicacearum, on Zn accumulation and speciation in Brassica juncea grown in soil amended with 600 mg kg-1 elemental Zn as three Zn species - soluble ZnSO4 and nanoparticles of ZnO and ZnS...
February 21, 2018: Environmental Science & Technology
https://www.readbyqxmd.com/read/29466092/initial-inoculation-concentration-does-not-affect-final-bacterial-colonization-of-in-vitro-vascular-conduits
#19
Thomas A Heafner, Clayton Lewis, Graham Baluh, Michael Clemens, Brandon Propper, Zachary M Arthurs
BACKGROUND: Despite improved peri-operative care, prosthetic graft infections continue to cause substantial morbidity and mortality. Contemporary graft infection models have tested a conduit's infectability using varying concentrations without standardization. Using a static assay in vitro model, we sought to evaluate the impact of inoculation concentration on vascular conduit attachment. METHODS: The 2-hour and 24-hour attachment of Staphylococcus aureus TCH1516 and Pseudomonas aeruginosa PA01-UW were determined on polytetrafluoroethylene (PTFE), Dacron® , nitinol, cobalt chromium, and Viabahn® (W...
February 21, 2018: Surgical Infections
https://www.readbyqxmd.com/read/29465342/lack-of-glyoxylate-shunt-dysregulates-iron-homeostasis-in-pseudomonas-aeruginosa
#20
Sunhee Ha, Bora Shin, Woojun Park
The aceA and glcB genes, encoding isocitrate lyase (ICL) and malate synthase, respectively, are not in an operon in many bacteria, including Pseudomonas aeruginosa, unlike in Escherichia coli. Here, we show that expression of aceA in P. aeruginosa is specifically upregulated under H2O2-induced oxidative stress and under iron-limiting conditions. In contrast, the addition of exogenous redox active compounds or antibiotics increases the expression of glcB. The transcriptional start sites of aceA under iron-limiting conditions and in the presence of iron were found to be identical by 5' RACE...
February 21, 2018: Microbiology
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