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Alaknanda J Adur, N Nandini, K Shilpashree Mayachar, R Ramya, N Srinatha
Silver nanoparticles were prepared through eco-friendly, cost effective, bio-mediated technique using anaerobically digested Parthenium hysterophorous digested slurry (PDS) for the first time. The synthesized nanoparticles were characterized through different techniques such as UV-Vis spectrophotometer for optical properties; X-ray diffractometer (XRD), high resolution transmission electron spectroscopy (HR-TEM) and Fourier Transform Infra Red (FTIR) Spectroscopy for structural property investigations. It was observed that the prepared silver nanoparticles were crystallized in face centered cubic crystal structure with an average particle size of 19 nm as confirmed from XRD...
April 16, 2018: Journal of Photochemistry and Photobiology. B, Biology
Rajapandiyan Krishnamoorthy, Jegan Athinarayanan, Vaiyapuri Subbarayan Periyasamy, Abdulraheem R Adisa, Mohammed A Al-Shuniaber, Mustafa A Gassem, Ali A Alshatwi
The appearance of drug-resistant (DR) bacteria in the community is a crucial development, and is associated with increased morbidity, mortality, healthcare costs, and antibiotic use. Natural oil nanoemulsions (NEs) have potential for antimicrobial applications. In the present study, we determined the antimicrobial activity of an NE against DR bacterial pathogens in vitro. The NE comprised Cleome viscosa essential oil, Tween 80 nonionic surfactant, and water. We found that an NE with a droplet size of 7 nm and an oil:surfactant (v/v) ratio of 1:3 was effective against methicillin-resistant Staphylococcus aureus (MRSA), DR Streptococcus pyogenes, and DR extended-spectrum beta-lactamase (ESBL)-producing Escherichia coli, Klebsiella pneumoniae, and Pseudomonas aeruginosa...
April 20, 2018: Microbial Pathogenesis
Shanshan Li, Xiaorui Lou, Yueyang Xu, Xiaozhen Teng, Shiyou Che, Ruihua Liu, Mark Bartlam
The C5 pathway in bacteria is responsible for the synthesis of 5-aminolevulinic acid, which forms the core skeleton of cofactors required for metabolism. One of the key actors in this pathway is a pyridoxamine-5'-phosphate (PMP)/pyridoxal-5'-phosphate (PLP) dependent enzyme called glutamate-1-semialdehyde aminomutase (GSAM). In this study, we crystallized the expression product of the uncharacterized pa4088 gene from the opportunistic pathogen Pseudomonas aeruginosa PAO1. The resulting high-resolution structure confirms it to be a member of the GSAM family...
April 20, 2018: Biochemical and Biophysical Research Communications
Dusan Milivojevic, Neven Šumonja, Strahinja Medic, Aleksandar Pavic, Ivana Moric, Branka Vasiljevic, Lidija Senerovic, Jasmina Nikodinovic-Runic
Pseudomonas aeruginosa, has been amongst top 10 'superbugs' worldwide and is causing infections with poor outcomes in both humans and animals. From 202 P. aeruginosa isolates (n = 121 animal and n = 81 human) 40 were selected on the basis of biofilm-forming ability and were comparatively characterized in terms of virulence determinants to the type strain P. aeruginosa PAO1. Biofilm formation, pyocyanin and hemolysins production and bacterial motility patterns were compared to the ability to kill human cell line A549 in vitro...
April 19, 2018: Pathogens and Disease
Isabelle J Schalk
In the alarming context of rising bacterial antibiotic resistance, there is an urgent need to discover new antibiotics or increase and/or enlarge the activity of those currently in use. The need for new antibiotics is even more urgent in the case of Gram-negative bacteria, such as Acinetobacter, Pseudomonas, and Enterobacteria, which have become resistant to many antibiotics and have an outer membrane with very low permeability to drugs. Vectorization of antibiotics using siderophores may be a solution to bypass such a bacterial wall: the drugs use the iron transporters of the outer membrane as gates to enter bacteria in a Trojan-horse strategy...
April 23, 2018: Journal of Medicinal Chemistry
Jo Ee Sam, Chee Loon Lim, Priya Sharda, Nasser Abdul Wahab
Introduction: Ventriculostomy-related infection (VRI) from external ventricular drain (EVD) insertion is a common complication and carries a high mortality rate. Choice of empiric antibiotics depends on the institutions common causative organisms and their susceptibility. We determined risk factors for mortality in patients with VRI, the common organisms causing VRI, and the rate of EVD-related VRI at our institution. Methods: Medical records and operative data of patients with cerebrospinal fluid positive cultures with an EVD inserted from 2012 to 2015 were traced...
April 2018: Asian Journal of Neurosurgery
Carla López-Causapé, Gabriel Cabot, Ester Del Barrio-Tofiño, Antonio Oliver
One of the most striking features of Pseudomonas aeruginosa is its outstanding capacity for developing antimicrobial resistance to nearly all available antipseudomonal agents through the selection of chromosomal mutations, leading to the failure of the treatment of severe hospital-acquired or chronic infections. Recent whole-genome sequencing (WGS) data obtained from in vitro assays on the evolution of antibiotic resistance, in vivo monitoring of antimicrobial resistance development, analysis of sequential cystic fibrosis isolates, and characterization of widespread epidemic high-risk clones have provided new insights into the evolutionary dynamics and mechanisms of P...
2018: Frontiers in Microbiology
Evangelia S Papadopoulou, Chiara Perruchon, Sotirios Vasileiadis, Constantina Rousidou, Georgia Tanou, Martina Samiotaki, Athanassios Molassiotis, Dimitrios G Karpouzas
Diphenylamine (DPA) is a common soil and water contaminant. A Pseudomonas putida strain, recently isolated from a wastewater disposal site, was efficient in degrading DPA. Thorough knowledge of the metabolic capacity, genetic stability and physiology of bacteria during biodegradation of pollutants is essential for their future industrial exploitation. We employed genomic, proteomic, transcription analyses and plasmid curing to (i) identify the genetic network of P. putida driving the microbial transformation of DPA and explore its evolution and origin and (ii) investigate the physiological response of bacterial cells during degradation of DPA...
2018: Frontiers in Microbiology
Jobina Rajkumari, Subhomoi Borkotoky, Ayaluru Murali, Kitlangki Suchiang, Saswat Kumar Mohanty, Siddhardha Busi
OBJECTIVE: Anti-quorum sensing and anti-biofilm efficacy of Cinnamic acid against Pseudomonas aeruginosa was comparatively assessed with respect to potent quorum sensing inhibitor, Baicalein. RESULTS: At sub-lethal concentration, Cinnamic acid effectively inhibited both the production of the QS-dependent virulence factors and biofilm formation in P. aeruginosa without affecting the viability of the bacterium. The phytocompound interfered with the initial attachment of planktonic cells to the substratum thereby causing reduction in biofilm development...
April 21, 2018: Biotechnology Letters
Hui Qing, Oscar Omondi Donde, Cuicui Tian, Chunbo Wang, Xingqiang Wu, Shanshan Feng, Yao Liu, Bangding Xiao
AD-1, an aerobic denitrifier, was isolated from activated sludge and identified as Pseudomonas stutzeri. AD-1 completely removed NO3 - or NO2 - and removed 99.5% of NH4 + during individual culturing in a broth medium with an initial nitrogen concentration of approximately 50 mg L-1 . Results showed that larger amounts of nitrogen were removed through assimilation by the bacteria. And when NH4 + was used as the sole nitrogen source in the culture medium, neither NO2 - nor NO3 - was detected, thus indicating that AD-1 may not be a heterotrophic nitrifier...
April 18, 2018: Journal of Bioscience and Bioengineering
Alessandro F Martins, Suelen P Facchi, Paulo C F da Câmara, Samira E A Camargo, Carlos H R Camargo, Ketul C Popat, Matt J Kipper
Poly(ε-caprolactone) (PCL) is a hydrophobic and cytocompatible aliphatic polyester that has been used to produce PCL-based nanofibrous for both wound healing and tissue repair. However, the high hydrophobicity and low water adsorptive have been challenges for developing PCL-based materials for use in tissue engineering field. Here, we report a new polymer (a hydrophilic amino-functionalized tannin (TN)) that is associated with PCL for developing PCL-TN blends at different PCL:TN weight ratios (100:0, 95:5, 85:15 and 78:22)...
April 16, 2018: Journal of Colloid and Interface Science
Alejandra Díaz-García, Ana R Borrero-Santiago, Inmaculada Riba
Two marine bacterial populations (Roseobacter sp. and Pseudomonas litoralis) were exposed to different concentrations of zinc (300, 625, 1250, 2000, 2500 and 5000 mg L-1 ) and cadmium (75, 250, 340, 500 and 1000 mg L-1 ) using two culture media (full nutrient Marine Broth 2216 "MB" and 1:10 (vol/vol) dilution with seawater of Marine Broth 2216 "MBSW "), in order to assess population responses depending on the culture medium and also potential adverse effects associated with these two metals...
April 14, 2018: Chemosphere
Oded Breuer, Daan Caudri, Lauren Akesson, Sarath Ranganathan, Stephen M Stick, André Schultz
In children with cystic fibrosis (CF) the associations between oropharyngeal swabs (OPS) for detection of Pseudomonas and lung disease has not been evaluated.OPS and bronchoalveolar lavage (BAL) samples were obtained annually in children with CF from 2005 to 2017. OPS test characteristics were calculated using BAL as gold standard. Results were related to lung inflammation (BAL neutrophil elastase, interleukin-8), structural lung disease (chest CT PRAGMA-CF scores), respiratory exacerbations, and future detection of Pseudomonas on BAL...
April 20, 2018: European Respiratory Journal: Official Journal of the European Society for Clinical Respiratory Physiology
Gianna Panasia, Bodo Philipp
The opportunistic pathogen Pseudomonas aeruginosa strain PAO1 is able to use a variety of organic pollutants as growth substrates including the anionic detergent sodium dodecyl sulfate (SDS) and long-chain alkanes. While the enzymes initiating SDS and alkane degradation are well known, the following enzymatic steps for degradation of the derived primary long-chain alcohols have not been identified yet. By evaluating genes specifically induced during growth with SDS, a gene cluster encoding a putative alcohol dehydrogenase (PA0364/LaoA), a probable inner membrane protein (PA0365/LaoB) and a presumable aldehyde dehydrogenase (PA0366/LaoC) were identified and designated as Lao (<u>l</u>ong-chain-<u>a</u>lcohol/<u>a</u>ldehyde-<u>o</u>xidation) system...
April 20, 2018: Applied and Environmental Microbiology
Egon A Ozer
BACKGROUND: The non-conserved accessory genome of bacteria can be associated with important adaptive characteristics that can contribute to niche specificity or pathogenicity of strains. High degrees of structural and compositional diversity in genomic islands and other elements of the accessory genome can complicate characterization of accessory genome contents among populations of strains. Methods for easily and effectively defining the distributions of discrete elements of the accessory genome among bacterial strains in a population are needed to explore the relationships between the flexible genome and bacterial adaptive traits...
April 20, 2018: BMC Bioinformatics
Abbass Jafari Kang, Alistair K Brown, Charles S Wong, Zhiyong Huang, Qiuyan Yuan
The treatment performance and bacterial community structure of conventional activated sludge and aerobic granules exposed to antibiotic sulfamethoxazole (SMX) was studied. For three months, two sets of sequencing batch reactors inoculated with conventional and granular biomass were fed with a synthetic municipal wastewater containing 2 μg/L SMX. The presence of SMX had no significant impacts on treatment performance of the reactors as well as stability of the granules. Results confirmed different bacterial community structure of flocs and granules...
April 13, 2018: Bioresource Technology
Yu-Wen Zhao, Hai-Xia Wang, Song-Tao Bie, Qian Shao, Chun-Hua Wang, Dong-Heng Wang, Zheng Li
A new method for detection of Escherichia coli exist in licorice decoction was developed by using DNA-based electrochemical biosensor. The thiolated capture probe was immobilized on a gold electrode at first. Then the aptamer for Escherichia coli was combined with the capture probe by hybridization. Due to the stronger interaction between the aptamer and the E. coli, the aptamer can dissociate from the capture probe in the presence of E. coli in licorice decoction. The biotinylated detection probe was hybridized with the single-strand capture probe...
March 2018: Zhongguo Zhong Yao za Zhi, Zhongguo Zhongyao Zazhi, China Journal of Chinese Materia Medica
Eugene M Obeng, Tatjana Brossette, Clarence M Ongkudon, Cahyo Budiman, Ruth Maas, Joachim Jose
This article comparatively reports the workability of Escherichia coli BL21(DE3) and Pseudomonas putida KT2440 cell factories for the expression of three model autodisplayed cellulases (i.e., endoglucanase, BsCel5A; exoglucanase, CelK; β-glucosidase, BglA). The differentiation of the recombinant cells was restricted to their cell growth and enzyme expression/activity attributes. Comparatively, the recombinant E. coli showed higher cell growth rates but lower enzyme activities than the recombinant P. putida...
April 19, 2018: Applied Microbiology and Biotechnology
Rikuan Zheng, Shimei Wu, Ning Ma, Chaomin Sun
Mercury-mediated toxicity remains one of the greatest barriers against microbial survival, even though bacterial resistance to mercury compounds can occur. However, the genetic and physiological adaptations of bacteria to mercury stress still remains unclear. Here, we show that the marine bacterium Pseudomonas stutzeri 273 is resistant to 50 μM Hg2+ and removes up to 94% Hg2+ from culture. Using gene homologous recombination and complementation, we show that genes encoding Hg2+ -transport proteins MerT, MerP, the mercuric reductase MerA and the regulatory protein MerD are essential for bacterial mercuric resistance when challenged with Hg2+ ...
2018: Frontiers in Microbiology
Giuseppe Firrao, Emanuela Torelli, Cesare Polano, Patrizia Ferrante, Francesca Ferrini, Marta Martini, Simone Marcelletti, Marco Scortichini, Paolo Ermacora
Pseudomonas syringae pv. actinidiae (Psa) biovar 3 caused pandemic bacterial canker of Actinidia chinensis and Actinidia deliciosa since 2008. In Europe, the disease spread rapidly in the kiwifruit cultivation areas from a single introduction. In this study, we investigated the genomic diversity of Psa biovar 3 strains during the primary clonal expansion in Europe using single molecule real-time (SMRT), Illumina and Sanger sequencing technologies. We recorded evidences of frequent mobilization and loss of transposon Tn6212, large chromosome inversions, and ectopic integration of IS sequences (remarkably ISPsy31, ISPsy36, and ISPsy37)...
2018: Frontiers in Microbiology
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