keyword
https://read.qxmd.com/read/38534415/a-keratin-12-expression-based-analysis-of-stem-precursor-cells-and-differentiation-in-the-limbal-corneal-epithelium-using-single-cell-rna-seq-data
#1
JOURNAL ARTICLE
J Mario Wolosin
The corneal epithelium (CE) is spread between two domains, the outer vascularized limbus and the avascular cornea proper. Epithelial cells undergo constant migration from the limbus to the vision-critical central cornea. Coordinated with this migration, the cells undergo differentiation changes where a pool of unique stem/precursor cells at the limbus yields the mature cells that reach the corneal center. Differentiation is heralded by the expression of the corneal-specific Krt12. Processing data acquired by scRNA-Seq showed that the increase in Krt12 expression occurs in four distinct steps within the limbus, plus a single continuous increase in the cornea...
February 26, 2024: Biology
https://read.qxmd.com/read/38534414/the-role-of-insulin-like-growth-factor-igf-system-in-the-corneal-epithelium-homeostasis-from-limbal-epithelial-stem-cells-to-therapeutic-applications
#2
REVIEW
Małgorzata Woronkowicz, Harry Roberts, Piotr Skopiński
The corneal epithelium, comprising three layers of cells, represents the outermost portion of the eye and functions as a vital protective barrier while concurrently serving as a critical refractive structure. Maintaining its homeostasis involves a complex regenerative process facilitated by the functions of the lacrimal gland, tear film, and corneal nerves. Crucially, limbal epithelial stem cells located in the limbus (transitional zone between the cornea and the conjunctiva) are instrumental for the corneal epithelium integrity by replenishing and renewing cells...
February 25, 2024: Biology
https://read.qxmd.com/read/38525980/ocular-chemical-injuries-and-limbal-stem-cell-deficiency-lscd-an-update-on-management
#3
JOURNAL ARTICLE
Yandong Bian, Ula Jurkunas
No abstract text is available yet for this article.
April 1, 2024: International Ophthalmology Clinics
https://read.qxmd.com/read/38514716/single-nuclei-transcriptomics-of-the-in-situ-human-limbal-stem-cell-niche
#4
JOURNAL ARTICLE
Kathryn C Davidson, Minkyung Sung, Karl D Brown, Julian Contet, Serena Belluschi, Regan Hamel, Aida Moreno-Moral, Rodrigo L Dos Santos, Julian Gough, Jose M Polo, Mark Daniell, Geraint J Parfitt
The corneal epithelium acts as a barrier to pathogens entering the eye; corneal epithelial cells are continuously renewed by uni-potent, quiescent limbal stem cells (LSCs) located at the limbus, where the cornea transitions to conjunctiva. There has yet to be a consensus on LSC markers and their transcriptome profile is not fully understood, which may be due to using cadaveric tissue without an intact stem cell niche for transcriptomics. In this study, we addressed this problem by using single nuclei RNA sequencing (snRNAseq) on healthy human limbal tissue that was immediately snap-frozen after excision from patients undergoing cataract surgery...
March 21, 2024: Scientific Reports
https://read.qxmd.com/read/38502347/-modified-mini-slet-for-treatment-of-complex-cases-in-pterygium-surgery
#5
JOURNAL ARTICLE
Luise Hahner, Juliane Jakob-Girbig, Somar Hasan, Daniel Meller
BACKGROUND: The major problem associated with the benign but destructive growing pterygium is the high recurrence rate. A new surgical technique to lower recurrence rates is minor ipsilateral simple limbal epithelial transplantation (mini-SLET), where the regeneration potential of limbal stem cells is used in combination with amniotic membrane transplantation (AMT) for surgical reconstruction. The aim of this study is to assess the surgical outcome of the mini-SLET technique with tenonectomy, mitomycin C, and AMT as used in the authors' hospital...
March 19, 2024: Ophthalmologie
https://read.qxmd.com/read/38482302/limbal-stem-cell-deficiency-demography-aetiology-and-clinical-presentation-in-eastern-india
#6
JOURNAL ARTICLE
Vivek K Singh, Neha Kumari, Rakhi Kusumesh, Bibhuti P Sinha
AIMS: To determine demographic details, clinical profile, and underlying causes of limbal stem cell deficiency. SETTINGS AND DESIGN: Descriptive cross-sectional study. METHODS AND MATERIAL: Patients visiting tertiary referral hospitals between January 2019 and December 2022 who satisfy the criteria for labeling limbal stem cell deficiency (LSCD) were enrolled. Parameters such as age, gender, region, duration, aetiology, and clinical features were noted...
January 2024: Journal of Family Medicine and Primary Care
https://read.qxmd.com/read/38478754/outcomes-of-simple-limbal-epithelial-transplantation-without-amniotic-membrane-grafting-in-unilateral-limbal-stem-cell-deficiency-a-case-series-of-6-patients
#7
JOURNAL ARTICLE
Neha Jain, Vikas Mittal, Dharma Sanandiya
PURPOSE: This study describes the technique of simple limbal epithelial transplantation (SLET) without amniotic membrane grafting (AMG) in limbal stem cell deficiency (LSCD). METHODS: Retrospective, interventional case series of 6 patients who underwent SLET without AMG were included. The procedure followed the standard technique, involving limbal biopsy from the healthy eye, resection of symblephera, and pannus dissection in the affected eye. Following host bed preparation, limbal explants were placed on the bare cornea and secured with fibrin glue...
March 13, 2024: Cornea
https://read.qxmd.com/read/38466286/substrate-stiffness-modulates-stemness-and-differentiation-of-rabbit-corneal-endothelium-through-the-paxillin-yap-pathway
#8
JOURNAL ARTICLE
Shuting Liu, Hua Chen, Huatao Xie, Xin Liu, Mingchang Zhang
PURPOSE: To explore the role of substrate stiffness and the mechanism beneath corneal endothelial cells' (CECs') stemness maintenance and differentiation. METHODS: CECs were divided into central zone (8 mm trephined boundary) and peripheral zone (8 mm trephined edge with attached limbal). Two zones were analyzed by hematoxylin-eosin staining and scanning electron microscopy for anatomic structure. The elastic modulus of Descemet's membrane (DM) was analyzed by atomic force microscopy...
March 5, 2024: Investigative Ophthalmology & Visual Science
https://read.qxmd.com/read/38444179/examination-of-subbasal-nerve-plexus-and-central-corneal-stromal-microstructure-in-subjects-with-congenital-aniridia-using-in-vivo-confocal-laser-scanning-microscopy
#9
JOURNAL ARTICLE
Anita Csorba, Kitti Kormányos, Mária Csidey, Annamária Náray, Klaudia Kovács, Orsolya Németh, Krisztina Knézy, Mária Bausz, Andrea Szigeti, Dorottya Szabó, Marta Corton, Kálmán Tory, Zoltán Zsolt Nagy, Achim Langenbucher, Erika Maka, Nóra Szentmáry
PURPOSE: During life up to 70% of aniridia subjects develop aniridia-associated keratopathy (AAK). AAK is characterized by limbal stem cell insufficiency, impaired corneal epithelial cell differentiation and abnormal cell adhesion, which leads to centripetal spreading vascularization, conjunctivalization, and thickening of the cornea. Our aim was to examine the subbasal nerve plexus and central corneal stromal microstructure in subjects with congenital aniridia, using in vivo confocal laser scanning microscopy CLSM...
March 5, 2024: Current Eye Research
https://read.qxmd.com/read/38412997/-not-available
#10
JOURNAL ARTICLE
Jens Heichel, Andrea Huth, Arne Viestenz, Hans-Gert Struck
Chemical burns of the ocular surface (CBOS) are emergencies of highest urgency. Therefore, an adequate emergency care is mandatory. Following a precise analysis of the initial damage, a staged therapeutic approach is used to prevent persistent impairment of the ocular surface. In the acute stage, the prevention of complications is targeted (symblepharon, conjunctival scarring, lacrimal stenosis, corneal ulceration, intraocular inflammation, elevated intraocular pressure, etc.). In later stages, if complications have developed, a secondary restoration of the ocular surface is focussed...
February 27, 2024: Klinische Monatsblätter Für Augenheilkunde
https://read.qxmd.com/read/38395213/isolation-and-characterization-of-rabbit-limbal-niche-cells
#11
JOURNAL ARTICLE
Guanyu Su, Xiaojie Guo, Lingjuan Xu, Bihui Jin, Yongyao Tan, Xiao Zhou, Wei Wang, Xinyu Li, Shusheng Wang, Guigang Li
Limbal niche cells (LNCs) are one of the most important supporting cells for corneal epithelial stem cells (CES), however, research on LNCs has been mostly limited to humans and rats previously. To expand the research work into the rabbit animal model, one of the most often used animals in stem cell study, this study was carried out for the in vitro isolation and identification of rabbit LNCs. Rabbit LNCs were isolated by collagenase A digestion method and single cells were obtained, the cells were then seeded on 5% Matrigel-coated plastic surface and cultured in modified embryonic stem cell medium (MESCM)...
February 21, 2024: Experimental Eye Research
https://read.qxmd.com/read/38378042/corneal-neurotization-essentials-for-the-facial-paralysis-surgeon
#12
JOURNAL ARTICLE
Jordan Crabtree, Chilando Mulenga, Khoa Tran, Arif Hussain, Charline S Boente, Asim Ali, Konstantin Feinberg, Gregory Borschel
Deficits in corneal innervation lead to neurotrophic keratopathy (NK). NK is frequently associated with facial palsy, and corneal damage can be accelerated by facial palsy deficits. Corneal nerves are important regulators of limbal stem cells, which play a critical role in epithelial maintenance and healing. Non-surgical treatments of NK have undergone recent innovation, and growth factors implicated in corneal epithelial renewal are a promising therapeutic avenue. However, surgical intervention with corneal neurotization (CN) remains the only definitive treatment of NK...
February 20, 2024: Facial Plastic Surgery: FPS
https://read.qxmd.com/read/38376237/letter-regarding-midterm-outcomes-of-autologous-glueless-simple-limbal-epithelial-transplantation-for-unilateral-limbal-stem-cell-deficiency
#13
JOURNAL ARTICLE
Siddharth Sharma, Abhishek Gupta, Sweksha Priya, Prabrisha Banerjee, Sandhya Kumari, Prabhakar Singh
No abstract text is available yet for this article.
February 16, 2024: Cornea
https://read.qxmd.com/read/38360335/correlation-between-anterior-chamber-angle-status-and-limbal-stem-cell-deficiency-in-primary-angle-closure-glaucoma
#14
JOURNAL ARTICLE
Jiewen Mao, Yujin Wang, Yuelan Gao, Shanshan Wan, Weiyan Jiang, Yumiao Pan, Yulin Yan, Yuyu Cong, Xiaoshuo Shi, Linying Huang, Yanning Yang
PURPOSE: To investigate the correlation between the opening and closing states of anterior chamber angle (ACA) and the density of limbal epithelial basal cells (LEBCs) in subjects with primary angle-closure glaucoma (PACG). DESIGN: Cross-sectional observational study. METHODS: A total of 54 eyes of 29 patients diagnosed with PACG were included in the study. 54 eyes from normal subjects were included as control. Automatic evaluation system for ultrasound biomicroscopy images of anterior chamber angle was used to assist ophthalmologists in identifying the opening or closing state of ACA, and the in vivo confocal microscopy (IVCM) was used to evaluate the density of LEBCs in different directions...
February 13, 2024: American Journal of Ophthalmology
https://read.qxmd.com/read/38355568/the-application-of-a-4d-printed-chitosan-based-stem-cell-carrier-for-the-repair-of-corneal-alkali-burns
#15
JOURNAL ARTICLE
Zibo Wang, Changqing Jiang, Yuqiao Fan, Xiaodan Hao, Yanhan Dong, Xinjia He, Jinning Gao, Yongchun Zhang, Meng Li, Mengyuan Wang, Yiming Liu, Wenhua Xu
BACKGROUND: Corneal alkali burns can lead to ulceration, perforation, and even corneal blindness due to epithelial defects and extensive cell necrosis, resulting in poor healing outcomes. Previous studies have found that chitosan-based in situ hydrogel loaded with limbal epithelium stem cells (LESCs) has a certain reparative effect on corneal alkali burns. However, the inconsistent pore sizes of the carriers and low cell loading rates have resulted in suboptimal repair outcomes. In this study, 4D bioprinting technology was used to prepare a chitosan-based thermosensitive gel carrier (4D-CTH) with uniform pore size and adjustable shape to improve the transfer capacity of LESCs...
February 14, 2024: Stem Cell Research & Therapy
https://read.qxmd.com/read/38349582/long-term-ocular-surface-stability-in-conjunctivolimbal-autograft-and-ocular-surface-safety-in-the-donor-eyes
#16
JOURNAL ARTICLE
Burcu Nurozler Tabakci, Ayşe Burcu, Züleyha Yalnız Akkaya, Evin Şıngar, Selma Ozbek-Uzman, Firdevs Örnek
PURPOSE: The current study is aimed to present the long-term results of the patients who underwent conjunctivolimbal autograft (CLAU) as the primary operation in unilateral limbal stem cell deficiency and the ocular surface safety of the donor eyes. The patients were followed up for five years or longer. METHODS: The records of all patients who underwent CLAU as the primary operation were retrospectively analyzed. Additional ocular surface operations, ocular surface stability, best-corrected visual acuity (BCVA), and ocular surface status of the donor eyes were investigated...
February 13, 2024: International Ophthalmology
https://read.qxmd.com/read/38346049/allogeneic-limbo-deep-anterior-lamellar-keratoplasty-limbo-dalk-a-novel-surgical-technique-in-corneal-stromal-disease-and-limbal-stem-cell-deficiency
#17
JOURNAL ARTICLE
Verena Schöneberger, Volkan Tahmaz, Mario Matthaei, Sigrid Roters, Simona L Schlereth, Friederike Schaub, Claus Cursiefen, Björn O Bachmann
PURPOSE: To describe a novel corneal surgical technique combining Deep Anterior Lamellar Keratoplasty (DALK) with grafting of allogeneic limbus (Limbo-DALK) for the treatment of eyes with corneal stromal pathology and limbal stem cell deficiency (LSCD). METHODS: Clinical records of six Limbo-DALKs performed in five patients diagnosed with LSCD and corneal stromal pathology requiring keratoplasty were retrospectively reviewed. All patients were diagnosed with LSCD due to various pathologies including thermal and chemical burns, congenital aniridia or chronic inflammatory ocular surface disease...
2024: PloS One
https://read.qxmd.com/read/38338948/two-photon-and-multiphoton-microscopy-in-anterior-segment-diseases-of-the-eye
#18
REVIEW
Merrelynn Hong, Shu Zhen Chong, Yun Yao Goh, Louis Tong
Two-photon excitation microscopy (TPM) and multiphoton fluorescence microscopy (MPM) are advanced forms of intravital high-resolution functional microscopy techniques that allow for the imaging of dynamic molecular processes and resolve features of the biological tissues of interest. Due to the cornea's optical properties and the uniquely accessible position of the globe, it is possible to image cells and tissues longitudinally to investigate ocular surface physiology and disease. MPM can also be used for the in vitro investigation of biological processes and drug kinetics in ocular tissues...
January 30, 2024: International Journal of Molecular Sciences
https://read.qxmd.com/read/38311159/consequences-of-exposure-to-particulate-matter-on-the-ocular-surface-mechanistic-insights-from-cellular-mechanisms-to-epidemiological-findings
#19
REVIEW
Phit Upaphong, Chanisa Thonusin, Ornpailin Wanichthanaolan, Nipon Chattipakorn, Siriporn C Chattipakorn
Exposure to air pollutants, especially in the case of particulate matter (PM), poses significant health risks throughout the body. The ocular surface is directly exposed to atmospheric PM making it challenging to avoid. This constant exposure makes the ocular surface a valuable model for investigating the impact of air pollutants on the eyes. This comprehensive review assembles evidence from across the spectrum, from in vitro and in vivo investigations to clinical studies, offering a thorough understanding of how PM10 and PM2...
February 2, 2024: Environmental Pollution
https://read.qxmd.com/read/38305421/severity-classification-of-limbal-stem-cell-failure-due-to-steven-johnson-syndrome-in-the-light-of-the-classification-consensus-of-limbal-stem-cell-deficiency
#20
JOURNAL ARTICLE
Zeynep Akgun, Melis Palamar, Sait Egrilmez, Ayse Yagci, Ozlem Barut Selver
OBJECTIVES: To examine and to understand the limbal stem-cell deficiency (LSCD) because of Steven-Johnson syndrome (SJS) in line with the new classification system for the first time in the literature. METHODS: Medical records of patients with LSCD because of SJS were reviewed retrospectively. In addition to demographic data and ophthalmologic or systemic findings, anterior segment photographs of the patients were reviewed retrospectively. Limbal stem-cell deficiency severity was graded according to the classification published by the Limbal Stem Cell Working Group...
February 1, 2024: Eye & Contact Lens
keyword
keyword
82251
1
2
Fetch more papers »
Fetching more papers... Fetching...
Remove bar
Read by QxMD icon Read
×

Save your favorite articles in one place with a free QxMD account.

×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"

We want to hear from doctors like you!

Take a second to answer a survey question.