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Klebsiella pneumoniae pneumonia

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https://www.readbyqxmd.com/read/28437685/rapid-label-free-identification-of-klebsiella-pneumoniae-antibiotic-resistant-strains-by-the-drop-coating-deposition-surface-enhanced-raman-scattering-method
#1
Youjin Cheong, Young Jin Kim, Heeyoon Kang, Samjin Choi, Hee Joo Lee
Although many methodologies have been developed to identify unknown bacteria, bacterial identification in clinical microbiology remains a complex and time-consuming procedure. To address this problem, we developed a label-free method for rapidly identifying clinically relevant multilocus sequencing typing-verified quinolone-resistant Klebsiella pneumoniae strains. We also applied the method to identify three strains from colony samples, ATCC70063 (control), ST11 and ST15; these are the prevalent quinolone-resistant K...
April 20, 2017: Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy
https://www.readbyqxmd.com/read/28437231/capsular-polysaccharide-types-and-virulence-related-traits-of-epidemic-kpc-producing-klebsiella-pneumoniae-isolates-in-a-chinese-university-hospital
#2
Yang Liu, Pan-Pan Liu, Lian-Hui Wang, Dan-Dan Wei, La-Gen Wan, Wei Zhang
Klebsiella pneumoniae is an important human pathogen associated with a variety of diseases and the prevalence of blaKPC carrying K. pneumoniae (KPC-Kp) is rapidly increasing. Capsule is an important virulence factor in K. pneumoniae. In this study, we determined to first systematically characterize capsular polysaccharide (CPS) and virulence traits in KPC-Kp strains. A total of 56 KPC-Kp isolates were recovered from clinical samples in a Chinese hospital, which were assigned to clonal lineages by multilocus sequence typing (MLST)...
February 24, 2017: Microbial Drug Resistance: MDR: Mechanisms, Epidemiology, and Disease
https://www.readbyqxmd.com/read/28437228/first-case-of-ndm-1-producing-klebsiella-pneumoniae-in-annaba-university-hospital-algeria
#3
Amel Abderrahim, Nassima Djahmi, Charlotte Pujol, Sabina Nedjai, Mohamed Cherif Bentakouk, Djamila Kirane-Gacemi, Mazouz Dekhil, Albert Sotto, Jean-Philippe Lavigne, Alix Pantel
AIM: The aim of this study was to characterize two carbapenem-resistant Klebsiella pneumoniae isolates recovered from urine samples in a patient hospitalized at Annaba University hospital (Algeria) in 2014. RESULTS: Two K. pneumoniae isolates were studied because they proved resistant to almost all antibiotics tested with a high level resistance to ertapenem (minimum inhibitory concentration = 32 mg/L). The results of modified Hodge test and combined disk test (ROSCO Diagnostica, Taastrup, Denmark) were positive...
February 22, 2017: Microbial Drug Resistance: MDR: Mechanisms, Epidemiology, and Disease
https://www.readbyqxmd.com/read/28435070/siderophores-in-clinical-isolates-of-klebsiella-pneumoniae-promote-ciprofloxacin-resistance-by-inhibiting-the-oxidative-stress
#4
Wenli Zhang, Ying Zhang, Xinxin Wang, Fengshu Ding, Yingmei Fu, Jizi Zhao, Wuqi Song, Ogutu James Opiyo, Fengmin Zhang, Xiaobei Chen
To explore the relevance of and understand the potential mechanisms behind the production of siderophores by clinical isolates of K. pneumoniae and ciprofloxacin (CIP) resistance, we divided the K. pneumoniae isolates into two groups based on bacterial siderophores productin: high siderophore-yielding group (39 strains) and low siderophore-yielding group (38 strains). The rate of CIP resistance in K. pneumoniae (27/39 = 69.23%) from the high siderophore-yielding group was significantly higher than that (16/38 = 42...
April 20, 2017: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/28435019/epidemiology-and-trends-in-the-antibiotic-susceptibilities-of-gram-negative-bacilli-isolated-from-patients-with-intra-abdominal-infections-in-the-asia-pacific-region-2010-2013
#5
Ya-Ting Chang, Geoffrey Coombs, Thomas Ling, V Balaji, Camilla Rodrigues, Hiroshige Mikamo, Min-Ja Kim, Datin Ganeswrie Rajasekaram, Myrna Mendoza, Thean Yen Tan, Pattarachai Kiratisin, Yuxing Ni, Weinman Barry, Yingchun Xu, Yen-Hsu Chen, Po-Ren Hsueh
This study was conducted to investigate the epidemiology and antimicrobial susceptibility patterns of Gram-negative bacilli (GNB) isolated from intra-abdominal infections (IAIs) in the Asia-Pacific region (APR) from 2010-2013. A total of 17 350 isolates were collected from 54 centres in 13 countries in the APR. The three most commonly isolated GNB were Escherichia coli (46.1%), Klebsiella pneumoniae (19.3%) and Pseudomonas aeruginosa (9.8%). Overall, the rates of extended-spectrum β-lactamase (ESBL)-producing E...
April 20, 2017: International Journal of Antimicrobial Agents
https://www.readbyqxmd.com/read/28434558/resistance-profiles-and-risk-factors-of-resistant-microorganisms-in-bacteraemia-of-abdominal-origin
#6
J Martín Jaramago, R Armero Ibáñez, J J Camarena Miñana, M Morales Suárez-Varela
OBJECTIVE: The presence of resistant microorganisms is a major cause of failure in initial empirical antimicrobial therapy. The objectives of this study are to determine the resistance profile of microorganisms that cause bacteraemia of abdominal origin and to identify whether the previous use of antibiotics and the place of acquisition of bacteraemia are risk factors associated with the presence of resistant organisms. MATERIAL AND METHODS: A clinical, observational, epidemiological, retrospective cohort study was conducted with all the adult patients admitted to a university hospital from 2011-2013...
April 20, 2017: Revista Española de Anestesiología y Reanimación
https://www.readbyqxmd.com/read/28432369/sensitivity-to-antibiotics-of-bacteria-exposed-to-gamma-radiation-emitted-from-hot-soils-of-the-high-background-radiation-areas-of-ramsar-northern-iran
#7
Seyed Mohammad Javad Mortazavi, Samira Zarei, Mohammad Taheri, Saeed Tajbakhsh, Seyed Alireza Mortazavi, Sahar Ranjbar, Fatemeh Momeni, Samaneh Masoomi, Leila Ansari, Mohammad Mehdi Movahedi, Shahram Taeb, Sina Zarei, Masood Haghani
BACKGROUND: Over the past several years our laboratories have investigated different aspects of the challenging issue of the alterations in bacterial susceptibility to antibiotics induced by physical stresses. OBJECTIVE: To explore the bacterial susceptibility to antibiotics in samples of Salmonella enterica subsp. enterica serovar Typhimurium (S. typhimurium), Staphylococcus aureus, and Klebsiella pneumoniae after exposure to gamma radiation emitted from the soil samples taken from the high background radiation areas of Ramsar, northern Iran...
April 2017: International Journal of Occupational and Environmental Medicine
https://www.readbyqxmd.com/read/28431848/epidemiology-of-device-associated-infections-in-an-intensive-care-unit-of-a-teaching-hospital-in-nepal-a-prospective-surveillance-study-from-a-developing-country
#8
Narayan Prasad Parajuli, Subhash Prasad Acharya, Santosh Dahal, Jaya Prasad Singh, Shyam Kumar Mishra, Hari Prasad Kattel, Basista Prasad Rijal, Bharat Mani Pokhrel
BACKGROUND: Device-associated health care-acquired infections (DA-HAIs) in intensive care unit patients are a major cause of morbidity, mortality, and increased health care costs. METHODS: A prospective, structured clinicomicrobiological surveillance was carried out for 3 common DA-HAIs: ventilator-associated pneumonia (VAP), central line-associated bloodstream infection (CLABSI), and catheter-associated urinary tract infection (CAUTI) present in the patients of an intensive care unit of a teaching hospital in Nepal...
April 18, 2017: American Journal of Infection Control
https://www.readbyqxmd.com/read/28426403/pcr-denaturing-gradient-gel-electrophoresis-as-a-useful-method-to-identify-of-intestinal-bacteria-flora-in-haemaphysalis-flava-ticks
#9
Tian-Yin Cheng, Guo-Hua Liu
Ticks are blood-sucking ectoparasites of great medical and veterinary significance that can transmit bacteria, protozoa, fungi and viruses, causing a variety of human and animal diseases worldwide. In the present study, the intestinal bacterial flora associated with Haemaphysalis flava ticks in different developmental stages were analyzed using polymerase chain reaction (PCR) and denaturing gradient gel electrophoresis (DGGE). Eleven distinct DGGE bands were found using PCR-DGGE method. Sequences analyses indicated that they belonged to Bacillus cereus, Candidatus rickettsia, Erwinia sp...
June 1, 2017: Acta Parasitologica
https://www.readbyqxmd.com/read/28426165/hydrogen-peroxide-dependent-antibacterial-action-of-melilotus-albus-honey
#10
Patrycja Sowa, Dorota Grabek-Lejko, Monika Wesołowska, Sylwia Swacha, Małgorzata Dżugan
Honey originating from different floral sources exhibits the broad-spectrum of antibacterial activity as a result of the presence of hydrogen peroxide as well as non peroxide bioactive compounds. The mechanisms of antibacterial activity of Polish melilot honey were investigated for the first time. Polish melilot honey samples (Melilotus albus biennal -3 and annual-5, Melilotus officinalis -1) were collected directly from beekeepers and analyzed for pollen profile, basic physicochemical parameters, antioxidant capacity, radical scavenging activity, total phenolics contents as well as antibacterial properties against pathogenic bacteria Staphylococcus aureus, Escherichia coli, Klebsiella pneumoniae, Salmonella spp...
April 20, 2017: Letters in Applied Microbiology
https://www.readbyqxmd.com/read/28425875/occurrence-of-qnrb1-and-qnrb12-genes-mutation-in-gyra-and-ramr-and-expression-of-efflux-pumps-in-isolates-of-klebsiella-pneumoniae-carriers-of-blakpc-2
#11
Alexsandra Maria Lima Scavuzzi, Maria Amélia Vieira Maciel, Heloísa Ramos Lacerda de Melo, Luiz Carlos Alves, Fábio André Brayner, Ana Catarina Souza Lopes
PURPOSE: The occurrence of quinolone-resistance genes (qnrA, qnrB and qnrS), the presence of mutations in gyrA, gyrB and parC, as well as the expression of efflux pumps (acrB and acrF) and mutations in the gene ramR. METHODOLOGY: Were investigated in 30 blaKPC-2-positive isolates of Klebsiella pneumoniae taken from infection and colonization in hospital patients from Recife-PE, Brazil. The detection of the qnr, acrB and acrF genes and analysis of the mutations in ramR and the quinolone-resistance-determining regions of gyrA, gyrB and parC were performed by PCR followed by DNA sequencing...
April 21, 2017: Journal of Medical Microbiology
https://www.readbyqxmd.com/read/28424761/sequencing-of-blaimp-carrying-incn2-plasmids-and-comparative-genomics-of-incn2-plasmids-harboring-class-1-integrons
#12
Xiaoyuan Jiang, Zhe Yin, Xiuyun Yin, Haihong Fang, Qiang Sun, Yigang Tong, Yuanhong Xu, Defu Zhang, Jiao Feng, Weijun Chen, Yajun Song, Jinglin Wang, Shuiping Chen, Dongsheng Zhou
This work presents the complete nucleotide sequences of p0801-IMP from Klebsiella pneumoniae, p7121-IMP from K. oxytoca, and p17285-IMP from Citrobacter freundii, which are recovered from three different cases of nosocomial infection. These three plasmids represent the first fully sequenced blaIMP-carrying IncN2 plasmids. Further comparative genomics analysis of all the five integron-carrying IncN2 plasmids p0801-IMP, p7121-IMP, p17285-IMP, pJIE137, and p34983-59.134kb indicates that they possess conserved IncN2 backbones with limited genetic variations with respect to gene content and organization...
2017: Frontiers in Cellular and Infection Microbiology
https://www.readbyqxmd.com/read/28424676/seasonal-fluctuations-of-microbial-aerosol-in-live-poultry-markets-and-the-detection-of-endotoxin
#13
Bo Wu, Kai Meng, Liangmeng Wei, Yumei Cai, Tongjie Chai
Microbial aerosol whose species and concentrations are closely related to human health is ubiquitous. The effect of microbes on human and animal health and production performance is, in many cases, caused by the spreading of air. Samples in this experiment were from a live poultry market (LPM) in Tai'an, China, collected three times a day (8 am, 14 pm, and 20 pm) over three consecutive days each month for 11 months (Original sampling plan was a year, the government due to the environmental protection, the was LPM closed)...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28424241/microbiota-induced-antibodies-are-essential-for-host-inflammatory-responsiveness-to-sterile-and-infectious-stimuli
#14
Daniel Cisalpino, Caio T Fagundes, Camila B Brito, Fernando R Ascenção, Celso M Queiroz-Junior, Angélica T Vieira, Lirlândia P Sousa, Flávio A Amaral, Leda Q Vieira, Jacques R Nicoli, Mauro M Teixeira, Danielle G Souza
The indigenous intestinal microbiota is frequently considered an additional major organ of the human body and exerts profound immunomodulating activities. Germ-free (GF) mice display a significantly different inflammatory responsiveness pattern compared with conventional (CV) mice, and this was dubbed a "hyporesponsive phenotype." Taking into account that the deposition of immune complexes is a major event in acute inflammation and that GF mice have a distinct Ig repertoire and B cell activity, we aimed to evaluate whether this altered Ig repertoire interferes with the inflammatory responsiveness of GF mice...
April 19, 2017: Journal of Immunology: Official Journal of the American Association of Immunologists
https://www.readbyqxmd.com/read/28422471/-epidemiological-surveillance-for-multidrug-resistant-microorganisms-in-a-general-icu
#15
A Fernández-Verdugo, J Fernández, D Escudero, L Cofiño, L Forcelledo, M Telenti, E García-Prieto, R Rodríguez-García, L Álvarez-García, A Pérez-García, C Rodríguez-Lucas, F Vazquez
OBJECTIVE: Multidrug resistant (MDR) microorganisms represent a threat for patients admitted in Intensive Care Units (ICUs). The objective of the present study is to analyse the results of epidemiological surveillance cultures for these microorganisms in one of these units. METHODS: General ICU. Retrospective analysis, descriptive statistics. Analysis of epidemiological surveillance cultures for MDR microorganisms in 2015. Studied microorganisms: Methicillin-resistant Staphylococcus aureus (MRSA), ESBL-and/or carbapenemase-producing Klebsiella pneumoniae (CESBL-KP) and MDR Acinetobacter baumannii (MDRAB)...
April 5, 2017: Revista Española de Quimioterapia: Publicación Oficial de la Sociedad Española de Quimioterapia
https://www.readbyqxmd.com/read/28422067/synthesis-characterization-antimicrobial-and-antiproliferative-activity-evaluation-of-cu-ii-co-ii-zn-ii-ni-ii-and-pt-ii-complexes-with-isoniazid-derived-compound
#16
Elena Pahonțu, Diana-Carolina Ilieș, Sergiu Shova, Camelia Oprean, Virgil Păunescu, Octavian Tudorel Olaru, Flavian Ștefan Rădulescu, Aurelian Gulea, Tudor Roșu, Doina Drăgănescu
Hydrazone complexes of Cu(II), Co(II), Zn(II), Ni(II) and Pt(II) with N-isonicotinoyl-N'-(3-metoxy-2 hydroxybenzaldehyde)-hydrazone (HL) were synthesized and characterized by different physico-chemical techniques including elemental and thermal analysis, magnetic susceptibility measurements, molar electric conductivity, as well as IR (infrared), ¹H-NMR and (13)C-NMR (hydrogen and carbon nuclear magnetic resonance, UV-Vis (ultraviolet-visible), FAB (fast atom bombardment), EPR (electron paramagnetic resonance), and mass spectroscopies...
April 19, 2017: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/28420276/the-influence-of-maternal-vaginal-flora-on-the-intestinal-colonization-in-newborns-and-3-month-old-infants
#17
Iwona Gabriel, Anita Olejek, Krystyna Stencel-Gabriel, Miroslaw Wielgoś
The role of maternal vaginal bacteria on the colonization of neonatal gut is still matter of discussion. Our aim was estimate the role of maternal vaginal flora on the development of intestinal flora in neonates and 3- months-old infants. 79 maternal-neonatal pairs were included in the study. Vaginal swabs were taken before the rupture of membranes after admission to the delivery ward. First neonatal stool (meconium) and stool at 3-months-old infants were collected and cultured. All samples were subject to a microbiological analysis for Streptococcus, Staphylococcus, Bifidobacterium, Clostridium (including Cl...
April 18, 2017: Journal of Maternal-fetal & Neonatal Medicine
https://www.readbyqxmd.com/read/28419869/allodemic-distribution-of-plasmids-co-harbouring-blactx-m-15-aac-6-ib-cr-qnrb-in-klebsiella-pneumoniae-is-the-main-source-of-extended-spectrum-%C3%AE-lactamases-in-uruguay-s-paediatric-hospital
#18
V Garcia-Fulgueiras, L Araujo, I Bado, N F Cordeiro, M I Mota, G Laguna, G Algorta, R Vignoli
OBJECTIVES: This study aimed to describe the characteristics of clinical isolates of extended-spectrum β-lactamase (ESBL)-producing enterobacteria (EPE) in Uruguay's paediatric hospital. METHODS: ESBLs, qnr alleles and aac(6')-Ib-cr were sought and characterised in EPE isolated between March 2010 and March 2012. Transfer of resistance determinants was assessed by conjugation. Incompatibility (Inc) groups, plasmid toxin-antitoxin systems (TAS) and plasmid size were determined in transconjugants...
April 15, 2017: Journal of Global Antimicrobial Resistance
https://www.readbyqxmd.com/read/28418318/carbapenem-resistant-enterobacter-cloacae-in-patients-from-the-us-veterans-health-administration-2006-2015
#19
Brigid M Wilson, Nadim G El Chakhtoura, Sachin Patel, Elie Saade, Curtis J Donskey, Robert A Bonomo, Federico Perez
We analyzed carbapenem-resistant Enterobacteriaceae (CRE) trends among patients from the US Veterans Health Administration (VHA). After the emergence of CRE in the eastern United States, resistance rates remained stable in Klebsiella pneumoniae but increased in Enterobacter cloacae complex, suggesting a "second epidemic". VHA offers a vantage point for monitoring nationwide CRE trends.
May 2017: Emerging Infectious Diseases
https://www.readbyqxmd.com/read/28418313/mcr-1-colistin-resistance-in-esbl-producing-klebsiella-pneumoniae-france
#20
Yvan Caspar, Mylène Maillet, Patricia Pavese, Gilles Francony, Jean-Paul Brion, Marie-Reine Mallaret, Richard Bonnet, Frédéric Robin, Racha Beyrouthy, Max Maurin
We report intestinal carriage of an extended-spectrum β-lactamase-producing Klebsiella pneumoniae strain with high-level resistance to colistin (MIC 24 mg/L) in a patient in France who had been hospitalized for fungal meningitis. The strain had the mcr-1 plasmid gene and an inactivated mgrB gene, which are associated with colistin resistance.
May 2017: Emerging Infectious Diseases
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